Robert Skoff

Summary

Affiliation: Wayne State University
Country: USA

Publications

  1. pmc Increased Plp1 gene expression leads to massive microglial cell activation and inflammation throughout the brain
    Carrie L Tatar
    Department of Anatomy and Cell Biology, Wayne State University, Detroit, MI 48201, U S A
    ASN Neuro 2:e00043. 2010
  2. ncbi request reprint Plasticity of neurons and glia following neonatal hypoxic-ischemic brain injury in rats
    Robert P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, 540 E Canfield, Detroit, MI 48201, USA
    Neurochem Res 32:331-42. 2007
  3. ncbi request reprint Hypoxic-ischemic injury results in acute disruption of myelin gene expression and death of oligodendroglial precursors in neonatal mice
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, 540 East Canfield, Detroit, MI 48201, USA
    Int J Dev Neurosci 19:197-208. 2001
  4. ncbi request reprint The myelin proteolipid protein gene modulates apoptosis in neural and non-neural tissues
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell Death Differ 11:1247-57. 2004
  5. ncbi request reprint Analyses of proteolipid protein mutants show levels of proteolipid protein regulate oligodendrocyte number and cell death in vitro and in vivo
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit MI 48201, USA
    Neurochem Res 29:2095-103. 2004
  6. pmc Sexual dimorphism of oligodendrocytes is mediated by differential regulation of signaling pathways
    Muthulekha Swamydas
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan, USA
    J Neurosci Res 87:3306-19. 2009
  7. ncbi request reprint Differential expression of apoptotic markers in jimpy and in Plp overexpressors: evidence for different apoptotic pathways
    M Cerghet
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Neurocytol 30:841-55. 2001
  8. ncbi request reprint Epigenetic factors up-regulate expression of myelin proteins in the dysmyelinating jimpy mutant mouse
    P E Knapp
    Department of Anatomy, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Neurobiol 29:138-50. 1996
  9. ncbi request reprint Analysis of myelin proteolipid protein and F0 ATPase subunit 9 in normal and jimpy CNS
    J A Benjamins
    Department of Neurology, Wayne State University School of Medicine, Detroit, Michigan 48201
    Neurochem Res 19:1013-22. 1994
  10. ncbi request reprint PPAR delta agonists stimulate oligodendrocyte differentiation in tissue culture
    I Saluja
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Glia 33:191-204. 2001

Research Grants

Collaborators

  • D S Skundric
  • P E Knapp
  • Inderjit Singh
  • X Yang
  • Mirela Cerghet
  • JAMES GRANNEMAN
  • C A Dyer
  • M Said Ghandour
  • John Barks
  • Patrizia Casaccia-Bonnefil
  • Jinzhu Li
  • Carrie L Tatar
  • Muthulekha Swamydas
  • Laura A Harsan
  • Denise A Bessert
  • Denise Bessert
  • Mireya Marin-Husstege
  • Aliya U Zaidi
  • Zhan Zhang
  • Walid Jalabi
  • W J Cho
  • Shayne E M Boucher
  • I Saluja
  • Harley Y Tse
  • Michael K Shaw
  • Xiaoqing Zhao
  • Ajaib S Paintlia
  • Sunita Appikatla
  • Patrick Poulet
  • Nathalie Parizel
  • Blandine Guignard
  • Michela Muggironi
  • Haiyan Xu
  • Chadwick Mullins
  • David Raban
  • Jennifer E Ong
  • Faye S Silverstein
  • Maryann Williamson
  • J S Hatfield
  • D G Drescher
  • D A Bessert
  • M J Drescher
  • Leon R Carlock
  • Maria A Cypher
  • J A Benjamins
  • D M Studzinski
  • T T Best
  • M K Vermeesch
  • E A Carrey
  • L Nedelkoska
  • W P Bartlett

Detail Information

Publications32

  1. pmc Increased Plp1 gene expression leads to massive microglial cell activation and inflammation throughout the brain
    Carrie L Tatar
    Department of Anatomy and Cell Biology, Wayne State University, Detroit, MI 48201, U S A
    ASN Neuro 2:e00043. 2010
    ....
  2. ncbi request reprint Plasticity of neurons and glia following neonatal hypoxic-ischemic brain injury in rats
    Robert P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, 540 E Canfield, Detroit, MI 48201, USA
    Neurochem Res 32:331-42. 2007
    ..While massive destruction of neurons and glia are striking events shortly after brain HI, neural cells re-express their intrinsic properties and attempt an anatomical recovery long after injury...
  3. ncbi request reprint Hypoxic-ischemic injury results in acute disruption of myelin gene expression and death of oligodendroglial precursors in neonatal mice
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, 540 East Canfield, Detroit, MI 48201, USA
    Int J Dev Neurosci 19:197-208. 2001
    ....
  4. ncbi request reprint The myelin proteolipid protein gene modulates apoptosis in neural and non-neural tissues
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell Death Differ 11:1247-57. 2004
    ..These studies show that the level of native PLP modulates the amount of PCD during normal development via a pH-dependent mechanism...
  5. ncbi request reprint Analyses of proteolipid protein mutants show levels of proteolipid protein regulate oligodendrocyte number and cell death in vitro and in vivo
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit MI 48201, USA
    Neurochem Res 29:2095-103. 2004
    ..The regulation of apoptosis by PLP gene expression occurs independently of myelination, indicating that the PLP gene has multiple primary functions...
  6. pmc Sexual dimorphism of oligodendrocytes is mediated by differential regulation of signaling pathways
    Muthulekha Swamydas
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan, USA
    J Neurosci Res 87:3306-19. 2009
    ..Given the greater fluctuation of neurosteroids in women than in men and the higher incidence of multiple sclerosis (MS) in women, these sexually dimorphic differences may contribute to differences in male and female MS lesions...
  7. ncbi request reprint Differential expression of apoptotic markers in jimpy and in Plp overexpressors: evidence for different apoptotic pathways
    M Cerghet
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Neurocytol 30:841-55. 2001
    ..Our data shows for the first time, in vivo, that mutations in Plp gene increase oligodendrocyte death by activating the caspase cascade but the trigger to upregulate this cascade follows different pathways...
  8. ncbi request reprint Epigenetic factors up-regulate expression of myelin proteins in the dysmyelinating jimpy mutant mouse
    P E Knapp
    Department of Anatomy, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Neurobiol 29:138-50. 1996
    ..These results also highlight the importance of environmental factors in controlling OL phenotype...
  9. ncbi request reprint Analysis of myelin proteolipid protein and F0 ATPase subunit 9 in normal and jimpy CNS
    J A Benjamins
    Department of Neurology, Wayne State University School of Medicine, Detroit, Michigan 48201
    Neurochem Res 19:1013-22. 1994
    ..This finding rules out the possibility that contaminating F0 ATPase gives rise to the DCCD binding exhibited by PLP and confirms the possibility that PLP has proton channel activity, as suggested by Lin and Lees (1,2)...
  10. ncbi request reprint PPAR delta agonists stimulate oligodendrocyte differentiation in tissue culture
    I Saluja
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Glia 33:191-204. 2001
    ..We show for the first time that a ligand that serves as an agonist for PPAR delta activates the program of OL differentiation in primary and enriched OL cultures...
  11. ncbi request reprint Death of individual oligodendrocytes in jimpy brain precedes expression of proteolipid protein
    M K Vermeesch
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Mich
    Dev Neurosci 12:303-15. 1990
    ..Alternatively, the PLP message may code for another protein which is important for normal development of neuroglia...
  12. ncbi request reprint Recovery of proteolipid protein in mice heterozygous for the jimpy gene
    J A Benjamins
    Department of Neurology, Wayne State University School of Medicine, Detroit, MI 48201
    J Neurochem 53:279-86. 1989
    ..abstract truncated at 250 words)..
  13. ncbi request reprint A defect in the cell cycle of neuroglia in the myelin deficient jimpy mouse
    P E Knapp
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201
    Brain Res 432:301-6. 1987
    ..Therefore, the unusually high L:M ratio indicates that there is an abnormality in some aspect of the jimpy oligodendroglial cell cycle...
  14. ncbi request reprint Differences in levels of neuroglial cell death in jimpy male mice and carrier females
    P E Knapp
    Department of Anatomy and Cell Biology, Wayne State University, Detroit, Mich
    Dev Neurosci 12:145-52. 1990
    ..Even if this occurs, the data still support the idea that the phenotype of genetically jp cells can be favorably altered by the mosaic environment...
  15. ncbi request reprint Comparison of in vivo and in vitro subcellular localization of estrogen receptors alpha and beta in oligodendrocytes
    Zhan Zhang
    Department of Anatomy and Cell Biology, Wayne State University, School of Medicine, Detroit, Michigan, USA
    J Neurochem 89:674-84. 2004
    ..Incubation of glial cultures without or with increasing concentrations of 17beta-estradiol (E2) shows that E2 significantly accelerates OLG process formation...
  16. pmc Differential levels of resistance to disease induction and development of relapsing experimental autoimmune encephalomyelitis in two H-2b-restricted mouse strains
    Jinzhu Li
    Department of Immunology and Microbiology, Wayne State University, School of Medicine, Detroit, MI 48201, USA
    J Neuroimmunol 234:109-14. 2011
    ..The new SJL.B mouse strain provides a valuable tool for studying EAE resistance and remitting-relapsing disease in H-2(b) mice...
  17. ncbi request reprint Oligodendrocytes in female carriers of the jimpy gene make more myelin than normal oligodendrocytes
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Comp Neurol 355:124-33. 1995
    ..These findings demonstrate that (1) a reduction in number of OLs does not necessarily involve a reduction in the amount of myelin, and (2) OLs have considerable flexibility in the amount of myelin they can make...
  18. ncbi request reprint The pH of jimpy glia is increased: intracellular measurements using fluorescent laser cytometry
    P E Knapp
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201
    Int J Dev Neurosci 11:215-26. 1993
    ..These results and others discussed in this paper support the hypothesis that either proteolipid protein itself, or some other product of the gene, may have an important role in central nervous system development...
  19. ncbi request reprint Jimpy mutation affects astrocytes: lengthening of the cell cycle in vitro
    P E Knapp
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Mich
    Dev Neurosci 15:31-6. 1993
    ..Since there is no evidence that astrocytes make myelin proteolipid protein, the results support previous studies which suggest that this gene may code for other proteins playing an important role in the development of many cell types...
  20. ncbi request reprint Expression of galactocerebroside in developing normal and jimpy oligodendrocytes in situ
    M S Ghandour
    Department of Anatomy and Cell Biology, Wayne State University, School of Medicine, Detroit, MI 48201
    J Neurocytol 17:485-98. 1988
    ..This finding shows that many cells in jimpy mice which were considered to be unclassifiable by the application of morphological criteria have, in fact, differentiated to the stage where they are galactocerebroside-positive...
  21. ncbi request reprint Astroglial plasticity in hemizygous and heterozygous jimpy mice
    T T Best
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201
    Int J Dev Neurosci 6:39-57. 1988
    ..These and other observations suggest that the glial hypertrophy in the young mosaic is temporary and that regression and reorganization of glial processes takes place as myelination proceeds...
  22. ncbi request reprint Programmed cell death in the dysmyelinating mutants
    R P Skoff
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Brain Pathol 5:283-8. 1995
    ..PCD of cells in normal development involving DNA fragmentation has been previously described and our results support that conclusion.(ABSTRACT TRUNCATED AT 250 WORDS)..
  23. ncbi request reprint Hyperpolarization-activated, cyclic AMP-gated, HCN1-like cation channel: the primary, full-length HCN isoform expressed in a saccular hair-cell layer
    W J Cho
    Department of Otolaryngology, Wayne State University School of Medicine, Lande Medical Research Building, 540 East Canfield Avenue, Detroit, MI 48201, USA
    Neuroscience 118:525-34. 2003
    ..The molecular properties of the HCN1-like isoform underlying I(h) expressed in the saccular hair cells of the teleost, trout, may consequently impact spontaneous release of transmitter from hair cells of the saccule...
  24. ncbi request reprint Astrocytic hypertrophy in dysmyelination influences the diffusion anisotropy of white matter
    Laura A Harsan
    UMR 7004 CNRS ULP, Institut de Physique Biologique, Strasbourg, France
    J Neurosci Res 85:935-44. 2007
    ....
  25. ncbi request reprint Proliferation and death of oligodendrocytes and myelin proteins are differentially regulated in male and female rodents
    Mirela Cerghet
    Department of Neurology, Henry Ford Hospital, Detroit, Michigan 48202, USA
    J Neurosci 26:1439-47. 2006
    ..We discuss the potential significance of these differences to multiple sclerosis, a sexually dimorphic disease, whose progression is altered by exogenous hormones...
  26. ncbi request reprint Oligodendrocyte progenitor proliferation and maturation is differentially regulated by male and female sex steroid hormones
    Mireya Marin-Husstege
    Department Neuroscience and Cell Biology, UMDNJ, Piscataway, NJ 08854, USA
    Dev Neurosci 26:245-54. 2004
    ..We conclude that sex steroids differentially regulate oligodendrocyte progenitor number and myelin formation, possibly contributing to gender-specific differences in repair...
  27. ncbi request reprint Anti-IL-16 therapy reduces CD4+ T-cell infiltration and improves paralysis and histopathology of relapsing EAE
    Dusanka S Skundric
    Department of Neurology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Neurosci Res 79:680-93. 2005
    ..We describe a novel therapeutic approach to specifically impede CD4+ T cell chemoattraction in EAE based on IL-16 neutralization. Our findings have high relevance for the development of new therapies for relapsing EAE and potentially MS...
  28. ncbi request reprint New oligodendrocytes are generated after neonatal hypoxic-ischemic brain injury in rodents
    Aliya U Zaidi
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Glia 46:380-90. 2004
    ..Although the neonatal brain undergoes massive cell death and atrophy the first week after injury, it retains the potential to generate new OLGs up to 4 weeks after injury within and surrounding the infarct...
  29. ncbi request reprint Proteolipid protein gene modulates viability and phenotype of neurons
    Shayne E M Boucher
    Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Neurosci 22:1772-83. 2002
    ..This effect of CM may be mediated by a negative pH shift elicited from PLP but not DM20 expression. These results indicate that PLP gene products directly modulate neuron viability...
  30. ncbi request reprint Trafficking of PLP/DM20 and cAMP signaling in immortalized jimpy oligodendrocytes
    M Said Ghandour
    UMR 7004 ULP CNRS, Strasbourg, France
    Glia 40:300-11. 2002
    ..The abnormal insertion of jimpy PLP/DM20 into the plasma membrane may be the basis for the lack of cell signaling and abnormal resting potential in jimpy oligodendrocytes...
  31. ncbi request reprint Detection of oligodendrocytes in tissue sections using PCR synthesis of digoxigenin-labeled probes
    Walid Jalabi
    UMR 7004 CNRS ULP, Faculte de Medecine, Universite Louis Pasteur, 11 rue Humann, 67085 Strasbourg, France
    J Histochem Cytochem 51:913-9. 2003
    ..This versatile and easy method for synthesis and labeling of specific probes to oligodendrocytes can be also applied to detect many other mRNAs in the nervous system and in other tissues...

Research Grants8

  1. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert Skoff; Fiscal Year: 2000
    ..Specific Aim 2: Determine why over expression of normal PLP and normal DM20 proteins induce PCD and if these proteins are modified by ubiquitin. Examine the transport of PLP to the cell membrane by DM20. ..
  2. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert Skoff; Fiscal Year: 2001
    ..Specific Aim 2: Determine why over expression of normal PLP and normal DM20 proteins induce PCD and if these proteins are modified by ubiquitin. Examine the transport of PLP to the cell membrane by DM20. ..
  3. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert Skoff; Fiscal Year: 2002
    ..Specific Aim 2: Determine why over expression of normal PLP and normal DM20 proteins induce PCD and if these proteins are modified by ubiquitin. Examine the transport of PLP to the cell membrane by DM20. ..
  4. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert Skoff; Fiscal Year: 2006
    ....
  5. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert Skoff; Fiscal Year: 2007
    ....
  6. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert Skoff; Fiscal Year: 2009
    ..abstract_text> ..
  7. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert P Skoff; Fiscal Year: 2010
    ..abstract_text> ..