BHANU JENA

Summary

Affiliation: Wayne State University
Country: USA

Publications

  1. doi request reprint Porosome: the secretory portal
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, Detroit, MI 48201, USA
    Exp Biol Med (Maywood) 237:748-57. 2012
  2. doi request reprint Porosome: the secretory NanoMachine in cells
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
    Methods Mol Biol 931:345-65. 2013
  3. ncbi request reprint Fusion pore or porosome: structure and dynamics
    B P Jena
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, Michigan 48201 4177, USA
    J Endocrinol 176:169-74. 2003
  4. pmc Secretion machinery at the cell plasma membrane
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201 4177, USA
    Curr Opin Struct Biol 17:437-43. 2007
  5. ncbi request reprint Discovery of the Porosome: revealing the molecular mechanism of secretion and membrane fusion in cells
    B P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Cell Mol Med 8:1-21. 2004
  6. ncbi request reprint Porosome: the universal molecular machinery for cell secretion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Mol Cells 26:517-29. 2008
  7. ncbi request reprint Molecular machinery and mechanism of cell secretion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201 4177, USA
    Exp Biol Med (Maywood) 230:307-19. 2005
  8. doi request reprint Porosome: the secretory portal in cells
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 48:4009-18. 2009
  9. pmc Structure and composition of the fusion pore
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biophys J 84:1337-43. 2003
  10. ncbi request reprint Cell secretion machinery: studies using the AFM
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201, USA
    Ultramicroscopy 106:663-9. 2006

Research Grants

Collaborators

  • L L Anderson
  • Akm A Sattar
  • Aleksandar Jeremic
  • Won Jin Cho
  • Sang Joon Cho
  • Rania Abu-Hamdah
  • Marie Kelly
  • Marie L Kelly
  • Alina Elena Ilie
  • Jin Ah Cho
  • Marvin H Stromer
  • Jeffrey J Potoff
  • W J Cho
  • Michael L Eisenberg
  • Anthony S Quinn
  • Douglas J Taatjes
  • J K Heinrich Horber
  • Sudhansu Dash
  • S J Cho
  • Jinah Cho
  • G Ren
  • Charles W Manke
  • Zeena Issa
  • Huan Jin
  • Gang Ren
  • J K H Hörber
  • John P Geibel
  • Jaimie D Nathan
  • Ajay V Maker
  • Kumudesh C Sritharan
  • Lori A Slezak
  • Dana K Andersen
  • Kathy T Rognlien
  • Ilia Lazrishvili
  • Aleksander Jeremic
  • Mzia G Zhvania
  • Bikashvili Tamar
  • Mary Sue Mayes
  • Eun Hwan Jeong
  • Mylan Satchi
  • Katherine T Rognlien
  • A Wakade
  • Aleksandra Glavaski
  • Ksenija Jeftinija
  • G D Pappas
  • Srdija Jeftinija

Detail Information

Publications44

  1. doi request reprint Porosome: the secretory portal
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, Detroit, MI 48201, USA
    Exp Biol Med (Maywood) 237:748-57. 2012
    ..A brief background on porosome discovery, and our current understanding of its structure and function is summarized in this Minireview...
  2. doi request reprint Porosome: the secretory NanoMachine in cells
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
    Methods Mol Biol 931:345-65. 2013
    ....
  3. ncbi request reprint Fusion pore or porosome: structure and dynamics
    B P Jena
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, Michigan 48201 4177, USA
    J Endocrinol 176:169-74. 2003
    ..Our studies using atomic force microscopy on live exocrine and neuroendocrine cells demonstrate the presence of such plasma membrane pores, revealing their morphology and dynamics at near nm resolution and in real time...
  4. pmc Secretion machinery at the cell plasma membrane
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201 4177, USA
    Curr Opin Struct Biol 17:437-43. 2007
    ..In recent years, significant progress has been made in our understanding of these three key cellular activities required for cell secretion. The molecular machinery and mechanism involving them is summarized in this article...
  5. ncbi request reprint Discovery of the Porosome: revealing the molecular mechanism of secretion and membrane fusion in cells
    B P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Cell Mol Med 8:1-21. 2004
    ..The molecular mechanism of secretory vesicle fusion at the base of porosomes, and vesicle swelling, have been resolved. With these findings a new understanding of cell secretion has emerged and confirmed by a number of laboratories...
  6. ncbi request reprint Porosome: the universal molecular machinery for cell secretion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Mol Cells 26:517-29. 2008
    ....
  7. ncbi request reprint Molecular machinery and mechanism of cell secretion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201 4177, USA
    Exp Biol Med (Maywood) 230:307-19. 2005
    ..These findings reveal, for the first time, the universal molecular machinery and mechanism of secretion in cells...
  8. doi request reprint Porosome: the secretory portal in cells
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biochemistry 48:4009-18. 2009
    ..In this review, the discovery of the porosome complex and the molecular mechanism of its function and how this information provides a new understanding of cell secretion are discussed...
  9. pmc Structure and composition of the fusion pore
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biophys J 84:1337-43. 2003
    ..These studies reveal the fusion pore or porosome to be a cup-shaped lipoprotein structure, the base of which has t-SNAREs and allows for docking and release of secretory products from membrane-bound vesicles...
  10. ncbi request reprint Cell secretion machinery: studies using the AFM
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201, USA
    Ultramicroscopy 106:663-9. 2006
    ..The molecular mechanism of secretory vesicle swelling, and the fusion of opposing bilayers, i.e., the fusion of secretory vesicle membrane at the base of the porosome membrane, has also been resolved...
  11. doi request reprint Atomic force microscopy: Unraveling the fundamental principles governing secretion and membrane fusion in cells
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, Detroit, MI 48201, USA
    Ultramicroscopy 109:1094-104. 2009
    ....
  12. ncbi request reprint Cell secretion and membrane fusion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201 4177, USA
    Domest Anim Endocrinol 29:145-65. 2005
    ..The molecular mechanism of secretory vesicle fusion at the base of porosomes, and vesicle swelling, has also been resolved. These findings reveal the molecular mechanism of cell secretion...
  13. ncbi request reprint Membrane fusion: role of SNAREs and calcium
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Protein Pept Lett 16:712-7. 2009
    ..The t-/v-SNARE is a tight complex, who's disassembly requires an ATPase called NSF, which functions as a right-handed molecular motor...
  14. doi request reprint Functional organization of the porosome complex and associated structures facilitating cellular secretion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan, USA
    Physiology (Bethesda) 24:367-76. 2009
    ....
  15. doi request reprint Secretory vesicles transiently dock and fuse at the porosome to discharge contents during cell secretion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell Biol Int 34:3-12. 2010
    ..This paradigm shift in our understanding of cell secretion is briefly outlined here...
  16. doi request reprint Role of SNAREs in membrane fusion
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Adv Exp Med Biol 713:13-32. 2011
    ..Calcium is then able to bridge the closely apposed bilayers, leading to the release of water from hydrated Ca(2+) ions as well as the loosely coordinated water at phospholipid head groups, leading to membrane destabilization and fusion...
  17. ncbi request reprint New structure involved in transient membrane fusion and exocytosis
    S J Cho
    Department of Physiology and Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Ann N Y Acad Sci 971:254-6. 2002
    ..These studies demonstrate that "pits" and "depressions" are sites at the apical plasma membrane in live cells, where membrane-bound secretory vesicles dock and transiently fuse to release vesicular contents...
  18. doi request reprint EM 3D contour maps provide protein assembly at the nanoscale within the neuronal porosome complex
    W J Cho
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Microsc 232:106-11. 2008
    ..The isolation of intact porosomes for near-atomic resolution using cryo-electron diffraction measurements, is finally possible...
  19. ncbi request reprint Porosome: the fusion pore revealed by multiple imaging modalities
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
    Methods Mol Biol 319:295-316. 2006
    ....
  20. ncbi request reprint Secretory vesicle swelling by atomic force microscopy
    Sang Joon Cho
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
    Methods Mol Biol 319:317-30. 2006
    ..Our results demonstrate that AQPI associated at the zymogen granule membrane is involved in basal GTP-induced and G(alphai3)-mediated rapid gating of water into zymogen granules of the exocrine pancreas...
  21. ncbi request reprint The atomic force microscope in the study of membrane fusion and exocytosis
    Bhanu P Jena
    Departments of Physiology and Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Methods Cell Biol 68:33-50. 2002
  22. ncbi request reprint Secretory vesicles in live cells are not free-floating but tethered to filamentous structures: a study using photonic force microscopy
    Rania Abu-Hamdah
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E Canfield, Detroit, MI 48201, USA
    Ultramicroscopy 106:670-3. 2006
    ..This makes sense, since precision and regulation are the hallmarks of all cellular process, and therefore would hold true for the transport and localization of subcellular organelles such as secretory vesicles...
  23. ncbi request reprint Cholesterol is critical to the integrity of neuronal porosome/fusion pore
    Aleksandar Jeremic
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI, 48201, USA
    Ultramicroscopy 106:674-7. 2006
    ..Thus, cholesterol participates as an integral component of the neuronal porosome complex, and is required for its stability...
  24. ncbi request reprint Calcium drives fusion of SNARE-apposed bilayers
    Aleksandar Jeremic
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201, USA
    Cell Biol Int 28:19-31. 2004
    ..Hence, this study suggests that in the physiological state in cells, both SNAREs and calcium operate as the minimal fusion machinery...
  25. ncbi request reprint Regulation of the water channel aquaporin-1: isolation and reconstitution of the regulatory complex
    Rania Abu-Hamdah
    Department of Physiology, Wayne State University School of Medicine, 5239 Scott Hall, 540 E Canfield Avenue, Detroit, MI 48201 4177, USA
    Cell Biol Int 28:7-17. 2004
    ..Our results demonstrate a Galphai3-PLA2 mediated pathway and potassium channel involvement in AQP-1 regulation...
  26. ncbi request reprint The number of secretory vesicles remains unchanged following exocytosis
    Sang Joon Cho
    Department of Physiology and Pharmacology, Wayne State University School of Medicine, Detroit, Michigan, 48201, USA
    Cell Biol Int 26:29-33. 2002
    ..Parallel studies using electron microscopy, further confirmed our AFM results. These studies demonstrate that following stimulation of secretion, membrane-bound secretory vesicles transiently dock and fuse to release vesicular contents...
  27. ncbi request reprint Fusion pore in live cells
    Bhanu P Jena
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    News Physiol Sci 17:219-22. 2002
    ..Recent studies using atomic force microscopy demonstrate for the first time the presence of the fusion pore and reveal its morphology and dynamics at near-nanometer resolution and in real time...
  28. pmc SNAREs in opposing bilayers interact in a circular array to form conducting pores
    Sang Joon Cho
    Departments of Physiology and Pharmacology, Wayne State University School of Medicine, 540E Canfield Avenue, Detroit, MI 48201, USA
    Biophys J 83:2522-7. 2002
    ..Thus, t- and v-SNAREs are required to reside in opposing bilayers to allow appropriate t-/v-SNARE interactions leading to membrane fusion...
  29. ncbi request reprint G(alpha)(i3) in pancreatic zymogen granules participates in vesicular fusion
    Akm A Sattar
    Department of Physiology, Wayne State University School of Medicine, East Canfield Avenue, Detroit, Michigan 48201, USA
    J Biochem 131:815-20. 2002
    ..Our studies suggest a possible role for ZG-associated G(alpha)(i3) in ZG-PM fusion...
  30. ncbi request reprint Vesicle swelling regulates content expulsion during secretion
    Marie L Kelly
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E Canfield, Detroit, MI 48201, USA
    Cell Biol Int 28:709-16. 2004
    ..These direct observations of the requirement of secretory vesicle swelling in secretion, provides an understanding of the appearance of partially empty vesicles following the process...
  31. ncbi request reprint Direct interaction between SNAP-23 and L-type Ca2+ channel
    Won Jin Cho
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI, 48201, USA
    J Cell Mol Med 9:380-6. 2005
    ..In this study, using yeast two-hybrid and immunoanalysis, we demonstrate for the first time, direct interaction of SNAP-23 and a L-type Ca2+-channel at the plasma membrane in pancreas...
  32. ncbi request reprint Patch clamped single pancreatic zymogen granules: direct measurements of ion channel activities at the granule membrane
    Marie L Kelly
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Pancreatology 5:443-9. 2005
    ..The aim of this study was to investigate the electrical activity of single ZGs and verify the types of channels found within their membrane...
  33. ncbi request reprint Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy
    Sang Joon Cho
    Department of Physiology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA
    Endocrinology 143:1144-8. 2002
    ..This study documents for the first time the presence of these structures and their involvement in secretions in a neuroendocrine cell...
  34. ncbi request reprint Structure and dynamics of the fusion pore in live cells
    Sang Joon Cho
    Department of Physiology and Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Cell Biol Int 26:35-42. 2002
    ..This study confirms depressions to be the fusion pores in pancreatic acinar cells. High-resolution images of the fusion pore in live pancreatic acinar cells reveal the structure in much greater detail than has previously been observed...
  35. pmc Reconstituted fusion pore
    Aleksandar Jeremic
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Biophys J 85:2035-43. 2003
    ..Fusion pore reconstituted into liposomes and examined by TEM, revealed a cup-shaped basket-like morphology, and were functional, as demonstrated by their ability to fuse with isolated secretory vesicles...
  36. pmc Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles
    Sang Joon Cho
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Proc Natl Acad Sci U S A 99:4720-4. 2002
    ..Our results demonstrate that AQP1 associated at the ZG membrane is involved in basal as well as GTP-induced rapid gating of water in ZGs of the exocrine pancreas...
  37. ncbi request reprint Size of supramolecular SNARE complex: membrane-directed self-assembly
    Won Jin Cho
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Am Chem Soc 127:10156-7. 2005
    ..In the present study, this hypothesis was tested, and results from the study demonstrate that the size of the t-/v-SNARE complex is directly proportional to the vesicle diameter (R2 = 0.9725)...
  38. ncbi request reprint Addendum to "Regulation of the water channel aquaporin-1: isolation and reconstitution of the regulatory complex" [Cell Biol. Int. 2004(1):7-17]
    Rania Abu-Hamdah
    Department of Physiology, Wayne State University, School of Medicine, Detroit, MI 48201 4177, USA
    Cell Biol Int 28:421. 2004
  39. pmc Neuronal fusion pore assembly requires membrane cholesterol
    Won Jin Cho
    Department of Physiology, Wayne State University, School of Medicine, 5245 Scott Hall, 540 E Canfield, Detroit, MI 48201, USA
    Cell Biol Int 31:1301-8. 2007
    ..Here we show that although cholesterol is an integral component of the neuronal porosome and is required for maintaining its physical integrity and function, it has no influence on the conformation of the SNARE ring complex...
  40. ncbi request reprint Structure, isolation, composition and reconstitution of the neuronal fusion pore
    Won Jin Cho
    Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E Canfield, Detroit, MI 48201, USA
    Cell Biol Int 28:699-708. 2004
    ..These findings reveal the molecular mechanism of neurotransmitter release at the presynaptic membrane of nerve terminals...
  41. ncbi request reprint Insulin receptor (IR) and glucose transporter 2 (GLUT2) proteins form a complex on the rat hepatocyte membrane
    Michael L Eisenberg
    Department of Surgery, Yale University School of Medicine, USA
    Cell Physiol Biochem 15:51-8. 2005
    ..Fluorescence-resonance energy transfer studies further confirmed this association. We conclude that IR and GLUT2 form a receptor-transporter complex in hepatocytes, which forms a mechanism of insulin-mediated hepatic glucose regulation...
  42. ncbi request reprint Energy-dependent disassembly of self-assembled SNARE complex: observation at nanometer resolution using atomic force microscopy
    Aleksandar Jeremic
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Am Chem Soc 128:26-7. 2006
    ..This study demonstrates that, irrespective of their arrangement, both forms of the SNARE complex can be disassembled in the presence of NSF-ATP...
  43. ncbi request reprint Involvement of water channels in synaptic vesicle swelling
    Aleksandar Jeremic
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Exp Biol Med (Maywood) 230:674-80. 2005
    ..Results from the study demonstrate that water channels AQP1 and AQP6, and the heterotrimeric Go protein are associated with SVs and participate in their swelling...
  44. doi request reprint Ca2+-dimethylphosphate complex formation: providing insight into Ca2+-mediated local dehydration and membrane fusion in cells
    Jeffrey J Potoff
    Department of Chemical Engineering and Materials Science, College of Engineering, Wayne State University, Detroit, MI 48201, USA
    Cell Biol Int 32:361-6. 2008
    ..92A, which is in close agreement with the 2.8A SNARE-induced apposition established between opposing bilayers, reported earlier from X-ray diffraction measurements...

Research Grants9

  1. EXOCYTOSIS: USING NANOSURGERY AND BIOENGINEERING
    BHANU JENA; Fiscal Year: 2003
    ..These studies will further our understanding of the exocytotic fusion machinery in pancreatic acinar cells, contributing to our understanding of this vital cellular process. ..
  2. UNDERSTANDING MEMBRANE FUSION A BIOENGINEERING APPROACH
    BHANU JENA; Fiscal Year: 2004
    ..The biochemical composition of the "new structure" will be determined by microsequencing electrophoretically resolved proteins immunoisolated from brain tissue, using antibodies to the SNARE proteins. ..