GEORGE BRUSH

Summary

Affiliation: Wayne State University
Country: USA

Publications

  1. pmc Yeast IME2 functions early in meiosis upstream of cell cycle-regulated SBF and MBF targets
    George S Brush
    Department of Oncology, Wayne State University School of Medicine, Detroit, Michigan, United States of America
    PLoS ONE 7:e31575. 2012
  2. pmc Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response
    G S Brush
    Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Nucleic Acids Res 28:3725-32. 2000
  3. pmc The ATM homologue MEC1 is required for phosphorylation of replication protein A in yeast
    G S Brush
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 93:15075-80. 1996
  4. pmc Replication protein A is sequentially phosphorylated during meiosis
    G S Brush
    Program in Molecular Biology and Genetics, Karmanos Cancer Institute, Wayne State University, 110 East Warren Avenue, Detroit, MI 48201, USA
    Nucleic Acids Res 29:4808-17. 2001
  5. pmc Identification and characterization of a single-stranded DNA-binding protein from the archaeon Methanococcus jannaschii
    T J Kelly
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21210, USA
    Proc Natl Acad Sci U S A 95:14634-9. 1998
  6. ncbi request reprint The meiosis-specific protein kinase Ime2 directs phosphorylation of replication protein A
    Dawn M Clifford
    Program in Molecular Biology and Genetics, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 279:6163-70. 2004
  7. pmc Evidence of meiotic crossover control in Saccharomyces cerevisiae through Mec1-mediated phosphorylation of replication protein A
    Amy J Bartrand
    Barbara Ann Karmanos Cancer Institute and Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Genetics 172:27-39. 2006
  8. ncbi request reprint Mechanistic insight into the Cdc28-related protein kinase Ime2 through analysis of replication protein A phosphorylation
    Dawn M Clifford
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan, USA
    Cell Cycle 4:1826-33. 2005
  9. ncbi request reprint Distinct activities of the related protein kinases Cdk1 and Ime2
    Kara E Sawarynski
    Barbara Ann Karmanos Cancer Institute and Department of Pathology, Wayne State University School of Medicine, 3114 Prentis Center, 110 E Warren Ave, Detroit, MI 48201, USA
    Biochim Biophys Acta 1773:450-6. 2007
  10. ncbi request reprint DNA stimulates Mec1-mediated phosphorylation of replication protein A
    Amy J Bartrand
    Program in Molecular Biology and Human Genetics, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 279:26762-7. 2004

Research Grants

  1. Mechanism of Meclp in the Checkpoint Response
    GEORGE BRUSH; Fiscal Year: 2006

Collaborators

Detail Information

Publications10

  1. pmc Yeast IME2 functions early in meiosis upstream of cell cycle-regulated SBF and MBF targets
    George S Brush
    Department of Oncology, Wayne State University School of Medicine, Detroit, Michigan, United States of America
    PLoS ONE 7:e31575. 2012
    ..While Ime2 is required for Sic1 destruction during meiosis, evidence now suggests that Ime2 does not directly catalyze Sic1 phosphorylation to target it for destabilization as Cln1,-2/Cdk1 do during the cell cycle...
  2. pmc Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response
    G S Brush
    Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Nucleic Acids Res 28:3725-32. 2000
    ....
  3. pmc The ATM homologue MEC1 is required for phosphorylation of replication protein A in yeast
    G S Brush
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 93:15075-80. 1996
    ....
  4. pmc Replication protein A is sequentially phosphorylated during meiosis
    G S Brush
    Program in Molecular Biology and Genetics, Karmanos Cancer Institute, Wayne State University, 110 East Warren Avenue, Detroit, MI 48201, USA
    Nucleic Acids Res 29:4808-17. 2001
    ..Our results suggest that Mec1-dependent RPA phosphorylation could be involved in regulating recombination rather than cell cycle or meiotic progression...
  5. pmc Identification and characterization of a single-stranded DNA-binding protein from the archaeon Methanococcus jannaschii
    T J Kelly
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21210, USA
    Proc Natl Acad Sci U S A 95:14634-9. 1998
    ..This ssDNA-binding domain was presumably present in the common ancestor to all three major branches of life...
  6. ncbi request reprint The meiosis-specific protein kinase Ime2 directs phosphorylation of replication protein A
    Dawn M Clifford
    Program in Molecular Biology and Genetics, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 279:6163-70. 2004
    ..Our studies have identified a novel meiosis-specific reaction that targets a key protein required for DNA replication, repair, and recombination. This pathway could be important in differentiating mitotic and meiotic DNA metabolism...
  7. pmc Evidence of meiotic crossover control in Saccharomyces cerevisiae through Mec1-mediated phosphorylation of replication protein A
    Amy J Bartrand
    Barbara Ann Karmanos Cancer Institute and Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Genetics 172:27-39. 2006
    ..These results suggest a new function of Mec1 that operates through RPA to locally control reciprocal recombination...
  8. ncbi request reprint Mechanistic insight into the Cdc28-related protein kinase Ime2 through analysis of replication protein A phosphorylation
    Dawn M Clifford
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan, USA
    Cell Cycle 4:1826-33. 2005
    ..Therefore, the mechanism underlying Ime2 substrate recognition could differ from that of Cdc28...
  9. ncbi request reprint Distinct activities of the related protein kinases Cdk1 and Ime2
    Kara E Sawarynski
    Barbara Ann Karmanos Cancer Institute and Department of Pathology, Wayne State University School of Medicine, 3114 Prentis Center, 110 E Warren Ave, Detroit, MI 48201, USA
    Biochim Biophys Acta 1773:450-6. 2007
    ..Examination of other substrates both in vitro and in vivo also revealed differing specificities. These results indicate that Ime2 does not simply replace G1 cyclin-CDK complexes in promoting Sic1 degradation during meiosis...
  10. ncbi request reprint DNA stimulates Mec1-mediated phosphorylation of replication protein A
    Amy J Bartrand
    Program in Molecular Biology and Human Genetics, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 279:26762-7. 2004
    ..Our results provide insight into the mechanisms that function in vivo to specifically induce RPA phosphorylation upon initiation of DNA replication, repair, or recombination...

Research Grants5

  1. Mechanism of Meclp in the Checkpoint Response
    GEORGE BRUSH; Fiscal Year: 2006
    ..It is expected that these studies will provide significant insight into the molecular mechanisms underlying maintenance of the non-cancerous state under genotoxic conditions. ..