ASHOK BHAGWAT

Summary

Affiliation: Wayne State University
Country: USA

Publications

  1. ncbi request reprint Cooperation and competition in mismatch repair: very short-patch repair and methyl-directed mismatch repair in Escherichia coli
    Ashok S Bhagwat
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Mol Microbiol 44:1421-8. 2002
  2. pmc DNA base flipping by both members of the PspGI restriction-modification system
    Michael A Carpenter
    Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA
    Nucleic Acids Res 36:5417-25. 2008
  3. pmc Sequence-dependent enhancement of hydrolytic deamination of cytosines in DNA by the restriction enzyme PspGI
    Michael Carpenter
    Department of Chemistry, Wayne State University, Detroit, MI 48202 USA
    Nucleic Acids Res 34:3762-70. 2006
  4. pmc Efficient deamination of 5-methylcytosines in DNA by human APOBEC3A, but not by AID or APOBEC3G
    Priyanga Wijesinghe
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Nucleic Acids Res 40:9206-17. 2012
  5. pmc Kinetic studies of Escherichia coli AlkB using a new fluorescence-based assay for DNA demethylation
    Todd W Roy
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Nucleic Acids Res 35:e147. 2007
  6. pmc Phylogenomic identification of five new human homologs of the DNA repair enzyme AlkB
    Michal A Kurowski
    Bioinformatics Laboratory, International Institute of Molecular and Cell Biology, Trojdena 4, 02 109 Warsaw, Poland
    BMC Genomics 4:48. 2003
  7. ncbi request reprint DNA-cytosine deaminases: from antibody maturation to antiviral defense
    Ashok S Bhagwat
    Department of Chemistry, Wayne State University, 443 Chemistry Building, Detroit, MI 48202 3489, USA
    DNA Repair (Amst) 3:85-9. 2004
  8. pmc Human activation-induced cytidine deaminase causes transcription-dependent, strand-biased C to U deaminations
    Anjum Sohail
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Nucleic Acids Res 31:2990-4. 2003
  9. ncbi request reprint Protection of DNA by alpha/beta-type small, acid-soluble proteins from Bacillus subtilis spores against cytosine deamination
    Anjum Sohail
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    Biochemistry 41:11325-30. 2002
  10. pmc Mismatch repair in methylated DNA. Structure and activity of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4
    Peiying Wu
    Department of Biochemistry, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 278:5285-91. 2003

Research Grants

  1. 7th Annual Midwest DNA Repair Symposium
    ASHOK BHAGWAT; Fiscal Year: 2005
  2. TRANSCRIPTION--INDUCED MUTATIONS
    ASHOK BHAGWAT; Fiscal Year: 1999
  3. Transcription-induced Mutations
    ASHOK BHAGWAT; Fiscal Year: 2007
  4. Discovering New Human DNA Repair Genes by Bioinformatics
    ASHOK BHAGWAT; Fiscal Year: 2007
  5. Transcription-induced Mutations
    ASHOK BHAGWAT; Fiscal Year: 2006
  6. Transcription-induced Mutations
    ASHOK BHAGWAT; Fiscal Year: 2005
  7. Improving Phenylbutyrate-based Anticancer Therapy
    ASHOK BHAGWAT; Fiscal Year: 2005
  8. TRANSCRIPTION--INDUCED MUTATIONS
    ASHOK BHAGWAT; Fiscal Year: 2001
  9. TRANSCRIPTION--INDUCED MUTATIONS
    ASHOK BHAGWAT; Fiscal Year: 2000

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Cooperation and competition in mismatch repair: very short-patch repair and methyl-directed mismatch repair in Escherichia coli
    Ashok S Bhagwat
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Mol Microbiol 44:1421-8. 2002
    ..We review here recent information about the biochemistry of both repair processes and describe the balancing act performed by cells to optimize the competing processes during different phases of the bacterial life cycle...
  2. pmc DNA base flipping by both members of the PspGI restriction-modification system
    Michael A Carpenter
    Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA
    Nucleic Acids Res 36:5417-25. 2008
    ..We discuss the implications of these results for DNA binding by these enzymes and their evolutionary origin...
  3. pmc Sequence-dependent enhancement of hydrolytic deamination of cytosines in DNA by the restriction enzyme PspGI
    Michael Carpenter
    Department of Chemistry, Wayne State University, Detroit, MI 48202 USA
    Nucleic Acids Res 34:3762-70. 2006
    ..This prediction was validated by modeling the structure of PspGI-DNA complex based on the structure of the related enzyme Ecl18kI which is known to cause base-flipping...
  4. pmc Efficient deamination of 5-methylcytosines in DNA by human APOBEC3A, but not by AID or APOBEC3G
    Priyanga Wijesinghe
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Nucleic Acids Res 40:9206-17. 2012
    ..These and other results suggest that the human AID deaminates 5mC's only weakly because the 5-methyl group fits poorly in its DNA-binding pocket...
  5. pmc Kinetic studies of Escherichia coli AlkB using a new fluorescence-based assay for DNA demethylation
    Todd W Roy
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Nucleic Acids Res 35:e147. 2007
    ..The results show that AlkB demethylates 1mA and 3mC with comparable efficiencies and has only a modest preference for a single-stranded DNA substrate over its double-stranded DNA counterpart...
  6. pmc Phylogenomic identification of five new human homologs of the DNA repair enzyme AlkB
    Michal A Kurowski
    Bioinformatics Laboratory, International Institute of Molecular and Cell Biology, Trojdena 4, 02 109 Warsaw, Poland
    BMC Genomics 4:48. 2003
    ..This discovery was based on the prediction made by Aravind and Koonin that AlkB is a member of the 2OG-Fe2+ oxygenase superfamily...
  7. ncbi request reprint DNA-cytosine deaminases: from antibody maturation to antiviral defense
    Ashok S Bhagwat
    Department of Chemistry, Wayne State University, 443 Chemistry Building, Detroit, MI 48202 3489, USA
    DNA Repair (Amst) 3:85-9. 2004
    ..This article discusses the many questions raised regarding the role of these enzymes in protecting cells against infections, and about their possible roles in genome evolution and carcinogenesis...
  8. pmc Human activation-induced cytidine deaminase causes transcription-dependent, strand-biased C to U deaminations
    Anjum Sohail
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Nucleic Acids Res 31:2990-4. 2003
    ..It suggests that AID may be recruited to variable genes through transcription without the assistance of other proteins and that the strand bias in SHM may be caused by the preference of AID for the non-transcribed strand...
  9. ncbi request reprint Protection of DNA by alpha/beta-type small, acid-soluble proteins from Bacillus subtilis spores against cytosine deamination
    Anjum Sohail
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    Biochemistry 41:11325-30. 2002
    ....
  10. pmc Mismatch repair in methylated DNA. Structure and activity of the mismatch-specific thymine glycosylase domain of methyl-CpG-binding protein MBD4
    Peiying Wu
    Department of Biochemistry, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 278:5285-91. 2003
    ..Modeling studies suggest the mismatched target nucleotide will be flipped out into the active site where candidate residues for catalysis and substrate specificity are present...
  11. ncbi request reprint Transcription promotes guanine to thymine mutations in the non-transcribed strand of an Escherichia coli gene
    Joanna Klapacz
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    DNA Repair (Amst) 4:806-13. 2005
    ..These results suggest that the magnitudes of individual base substitutions and their relative numbers in other studies of mutational spectra may also be affected by transcription...
  12. ncbi request reprint Evaluation of molecular models for the affinity maturation of antibodies: roles of cytosine deamination by AID and DNA repair
    Mala Samaranayake
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    Chem Rev 106:700-19. 2006
  13. ncbi request reprint Lack of dependance of transcription-induced cytosine deaminations on protein synthesis
    Sanath Kumar Mokkapati
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Mutat Res 508:131-6. 2002
    ..Our results show that TIM is not dependent on efficient translation of mRNA and this helps eliminate certain models concerning the mechanism underlying TIM...
  14. pmc Transcription increases methylmethane sulfonate-induced mutations in alkB strains of Escherichia coli
    Douglas Fix
    Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA
    DNA Repair (Amst) 7:1289-97. 2008
    ..We propose that this is the underlying cause of its increased sensitivity to MMS and suggest that transcriptionally active DNA may be a preferred target for the action of alkylating agents that prefer single-stranded DNA...
  15. ncbi request reprint Is AID a monomer in solution?
    Ashok S Bhagwat
    DNA Repair (Amst) 7:349-50; author reply 351-2. 2008
  16. pmc Transcription-dependent increase in multiple classes of base substitution mutations in Escherichia coli
    Joanna Klapacz
    Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA
    J Bacteriol 184:6866-72. 2002
    ..C-to-T. A transversions dominated the spectrum in cells transcribing the gene. These results suggest that high levels of transcription promote many different spontaneous base substitutions in E. coli...
  17. pmc A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
    ..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes...
  18. ncbi request reprint Mismatch uracil glycosylase from Escherichia coli: a general mismatch or a specific DNA glycosylase?
    Rory J O'Neill
    Imperial College London, Department of Biological Sciences, Sir Alexander Flemming Building, South Kensington, London SW7 2AZ, United Kingdom
    J Biol Chem 278:20526-32. 2003
    ..This comparison of different substrates under single turnover conditions provides a rational basis for comparing substrates of MUG and we relate these conclusions to the known crystal structures of the enzyme and its catalytic mechanism...

Research Grants10

  1. 7th Annual Midwest DNA Repair Symposium
    ASHOK BHAGWAT; Fiscal Year: 2005
    ..This Symposium will provide excellent opportunity to those scientists who are unable to attend conferences on either U.S. coast because of the distance or the cost, to participate in a major conference. ..
  2. TRANSCRIPTION--INDUCED MUTATIONS
    ASHOK BHAGWAT; Fiscal Year: 1999
    ..coli. The results from this work should create a new perspective on the action of mutagens and provide a greater understanding of the process of transcript elongation. ..
  3. Transcription-induced Mutations
    ASHOK BHAGWAT; Fiscal Year: 2007
    ..Further, this system will be used to study the roles played by various DNA repair enzymes, DNA polymerases and accessory proteins in shaping the strand bias and spectrum of mutations responsible for antibody maturation. ..
  4. Discovering New Human DNA Repair Genes by Bioinformatics
    ASHOK BHAGWAT; Fiscal Year: 2007
    ..This integrated prediction-validation approach should be superior to a purely bioinformatics or a purely biochemical approach and may serve as a paradigm for searching biochemical functions in genomes of all organisms. ..
  5. Transcription-induced Mutations
    ASHOK BHAGWAT; Fiscal Year: 2006
    ..Further, this system will be used to study the roles played by various DNA repair enzymes, DNA polymerases and accessory proteins in shaping the strand bias and spectrum of mutations responsible for antibody maturation. ..
  6. Transcription-induced Mutations
    ASHOK BHAGWAT; Fiscal Year: 2005
    ..Further, this system will be used to study the roles played by various DNA repair enzymes, DNA polymerases and accessory proteins in shaping the strand bias and spectrum of mutations responsible for antibody maturation. ..
  7. Improving Phenylbutyrate-based Anticancer Therapy
    ASHOK BHAGWAT; Fiscal Year: 2005
    ..This work will create the tools necessary for the monitoring of tumor cells in live animals when they are treated with PB as part of combination chemotherapy. ..
  8. TRANSCRIPTION--INDUCED MUTATIONS
    ASHOK BHAGWAT; Fiscal Year: 2001
    ..coli. The results from this work should create a new perspective on the action of mutagens and provide a greater understanding of the process of transcript elongation. ..
  9. TRANSCRIPTION--INDUCED MUTATIONS
    ASHOK BHAGWAT; Fiscal Year: 2000
    ..coli. The results from this work should create a new perspective on the action of mutagens and provide a greater understanding of the process of transcript elongation. ..