Lihong V Wang

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc Time-reversed ultrasonically encoded optical focusing in biological tissue
    Puxiang Lai
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, 1 Brookings Drive, St Louis, Missouri 63130
    J Biomed Opt 17:030506. 2012
  2. pmc Photoacoustic tomography through a whole adult human skull with a photon recycler
    Liming Nie
    Washington University, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 17:110506. 2012
  3. pmc Neurovascular photoacoustic tomography
    Song Hu
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St Louis St Louis, MO, USA
    Front Neuroenergetics 2:10. 2010
  4. pmc Label-free photoacoustic microscopy of myocardial sheet architecture
    Chi Zhang
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St Louis, Missouri 63130
    J Biomed Opt 17:060506. 2012
  5. pmc Reflection-mode submicron-resolution in vivo photoacoustic microscopy
    Chi Zhang
    Washington University, Department of Biomedical Engineering, St Louis, Missouri 63130
    J Biomed Opt 17:020501. 2012
  6. pmc Detection, mapping, and quantification of single walled carbon nanotubes in histological specimens with photoacoustic microscopy
    Pramod K Avti
    Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York, United States of America
    PLoS ONE 7:e35064. 2012
  7. pmc Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent proteins
    Arie Krumholz
    1 Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St Louis, St Louis, MO 63130, USA 2
    Sci Rep 4:3939. 2014
  8. pmc In vivo photoacoustic tomography of total blood flow and potential imaging of cancer angiogenesis and hypermetabolism
    Junjie Yao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Technol Cancer Res Treat 11:301-7. 2012
  9. pmc Prospects of photoacoustic tomography
    Lihong V Wang
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130 4899, USA
    Med Phys 35:5758-67. 2008
  10. ncbi request reprint Boundary conditions in photoacoustic tomography and image reconstruction
    Lihong V Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, Texas 77843 3120, USA
    J Biomed Opt 12:014027. 2007

Research Grants

Collaborators

Detail Information

Publications132 found, 100 shown here

  1. pmc Time-reversed ultrasonically encoded optical focusing in biological tissue
    Puxiang Lai
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, 1 Brookings Drive, St Louis, Missouri 63130
    J Biomed Opt 17:030506. 2012
    ....
  2. pmc Photoacoustic tomography through a whole adult human skull with a photon recycler
    Liming Nie
    Washington University, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 17:110506. 2012
    ..Our results indicate that PAT can potentially be applied to in vivo human brain imaging...
  3. pmc Neurovascular photoacoustic tomography
    Song Hu
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St Louis St Louis, MO, USA
    Front Neuroenergetics 2:10. 2010
    ..Finally, potential research directions and associated technical challenges are discussed...
  4. pmc Label-free photoacoustic microscopy of myocardial sheet architecture
    Chi Zhang
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St Louis, Missouri 63130
    J Biomed Opt 17:060506. 2012
    ..Therefore, PAM has the potential for the functional imaging of sheet architecture in ex vivo perfused and viable hearts...
  5. pmc Reflection-mode submicron-resolution in vivo photoacoustic microscopy
    Chi Zhang
    Washington University, Department of Biomedical Engineering, St Louis, Missouri 63130
    J Biomed Opt 17:020501. 2012
    ..Capillaries were resolved better by the submicron-resolution PAM. Therefore, the submicron-resolution PAM is suitable for in vivo high-resolution imaging, or even subcellular imaging, of optical absorption...
  6. pmc Detection, mapping, and quantification of single walled carbon nanotubes in histological specimens with photoacoustic microscopy
    Pramod K Avti
    Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York, United States of America
    PLoS ONE 7:e35064. 2012
    ....
  7. pmc Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent proteins
    Arie Krumholz
    1 Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St Louis, St Louis, MO 63130, USA 2
    Sci Rep 4:3939. 2014
    ..Our results suggest iRFPs are genetically-encoded probes of choice for simultaneous photoacoustic imaging of several tissues or processes in vivo. ..
  8. pmc In vivo photoacoustic tomography of total blood flow and potential imaging of cancer angiogenesis and hypermetabolism
    Junjie Yao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Technol Cancer Res Treat 11:301-7. 2012
    ..In addition, the method was tested in vivo on a mouse ear. We believe that the proposed method has the potential to be used for cancer angiogenesis and hypermetabolism imaging...
  9. pmc Prospects of photoacoustic tomography
    Lihong V Wang
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130 4899, USA
    Med Phys 35:5758-67. 2008
    ....
  10. ncbi request reprint Boundary conditions in photoacoustic tomography and image reconstruction
    Lihong V Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, Texas 77843 3120, USA
    J Biomed Opt 12:014027. 2007
    ..Analogously to the method of images of sources, which is commonly used in forward problems, the ultrasonic detectors are imaged about the boundary to satisfy the boundary condition in the inverse problem...
  11. pmc Simultaneous functional photoacoustic and ultrasonic endoscopy of internal organs in vivo
    Joon Mo Yang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri, USA
    Nat Med 18:1297-1302. 2012
    ..Here we present simultaneous photoacoustic and ultrasonic dual-mode endoscopy and show its ability to image internal organs in vivo, thus illustrating its potential clinical application. ..
  12. ncbi request reprint Exact frequency-domain reconstruction for thermoacoustic tomography--II: Cylindrical geometry
    Yuan Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843 3120, USA
    IEEE Trans Med Imaging 21:829-33. 2002
    ..The effect of discrete spatial sampling on image quality is analyzed. An aliasing-proof reconstruction method is proposed. Numerical and experimental results are included...
  13. pmc Effects of acoustic heterogeneities on transcranial brain imaging with microwave-induced thermoacoustic tomography
    Xing Jin
    Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Med Phys 35:3205-14. 2008
    ..This work is important for obtaining good brain images when the thickness of the skull cannot be ignored...
  14. pmc In vivo molecular photoacoustic tomography of melanomas targeted by bioconjugated gold nanocages
    Chulhong Kim
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    ACS Nano 4:4559-64. 2010
    ..The in vivo PAT quantification of the amount of AuNCs accumulated in melanomas was further validated with inductively coupled plasma mass spectrometry (ICP-MS)...
  15. ncbi request reprint Imaging of high-intensity focused ultrasound-induced lesions in soft biological tissue using thermoacoustic tomography
    Xing Jin
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843 3120, USA
    Med Phys 32:5-11. 2005
    ..Gross pathologic photographs of the tissue samples confirmed the TAT images. This work demonstrates that TAT may potentially be used to image HIFU-induced lesions in biological tissues...
  16. ncbi request reprint Thermoacoustic tomography with correction for acoustic speed variations
    Xing Jin
    Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843 3120, USA
    Phys Med Biol 51:6437-48. 2006
    ..In this paper we develop a correction method based on ultrasonic transmission tomography (UTT) to improve the image quality of TAT. Numerical simulations and phantom experiments verify the effectiveness of this correction method...
  17. ncbi request reprint In vivo burn imaging using Mueller optical coherence tomography
    Milos Todorović
    Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, USA
    Opt Express 16:10279-84. 2008
    ..By using a decomposition algorithm developed by our group, we also mapped the local birefringence of the skin. The experimental results demonstrate the system's potential for in vivo burn-depth determination...
  18. ncbi request reprint Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy
    Jung Taek Oh
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, 77843 3120, USA
    J Biomed Opt 11:34032. 2006
    ..The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning...
  19. ncbi request reprint Image distortion in thermoacoustic tomography caused by microwave diffraction
    Changhui Li
    Biomedical Engineering Department, Washington University in St Louis, St Louis, Missouri 63130, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 77:031923. 2008
    ..Both numerical simulations and phantom experiments demonstrate this phenomenon. A method of partial correction is also provided...
  20. pmc Up-regulation of hypoxia-inducible factor 1 alpha and hemodynamic responses following massive small bowel resection
    Kathryn J Rowland
    Division of Pediatric Surgery, St Louis Children s Hospital, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    J Pediatr Surg 48:1330-9. 2013
    ..We therefore sought to determine the duration of resection-induced intestinal hypoxia and expression of hypoxia-inducible factors (HIFs) following SBR...
  21. ncbi request reprint Half-time image reconstruction in thermoacoustic tomography
    Mark A Anastasio
    Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA
    IEEE Trans Med Imaging 24:199-210. 2005
    ..Reconstructed images and numerical results produced from simulated and experimental TAT measurement data are employed to demonstrate these effects...
  22. pmc Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution
    Bin Rao
    Department of Biomedical Engineering, Optical Imaging Lab, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130 4899, USA
    Opt Lett 36:1137-9. 2011
    ..The corresponding 2D B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23 ± 0.03 μm for Au nanowire imaging, which is 2.0 times below the diffraction limit...
  23. ncbi request reprint Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer
    Roger J Zemp
    Washington University, Optical Imaging Laboratory, Department of Biomedical Engineering, Saint Louis, Missouri 63130, USA
    J Biomed Opt 12:010501. 2007
    ..It is anticipated that the system can be used for studying and diagnosing a number of diseases including cancer, atherosclerosis, dermatological disorders, and peripheral microvascular complications in diabetes...
  24. pmc Energy enhancement in time-reversed ultrasonically encoded optical focusing using a photorefractive polymer
    Yuta Suzuki
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, MO 63130, USA
    J Biomed Opt 17:080507-1. 2012
    ..As a result, we successfully imaged absorbing objects embedded in the middle plane of a tissue-mimicking phantom having an optical thickness of 120 scattering mean free paths...
  25. pmc Quantitative photoacoustic microscopy of optical absorption coefficients from acoustic spectra in the optical diffusive regime
    Zijian Guo
    Washington University, Department of Biomedical Engineering, Optical Imaging Laboratory, 1 Brookings Drive, Saint Louis, Missouri 63130, USA
    J Biomed Opt 17:066011. 2012
    ..Compared with the conventional amplitude method, the acoustic spectral method provides greater quantification accuracy in the optical diffusive regime. The limitations of the acoustic spectral method was also discussed...
  26. pmc In vivo three-dimensional photoacoustic imaging based on a clinical matrix array ultrasound probe
    Yu Wang
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 17:061208. 2012
    ..These results highlight the clinical potential of 3-D PA imaging for identification of sentinel lymph nodes for cancer staging in humans...
  27. ncbi request reprint Thermoacoustic and photoacoustic tomography of thick biological tissues toward breast imaging
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843 3120, USA
    Technol Cancer Res Treat 4:559-66. 2005
    ..Based on the intrinsic optical contrast between blood and chicken breast muscle, an embedded blood object that was 5 cm deep in the tissue was also detected using PAT at a wavelength of 1064 nm...
  28. pmc Realtime photoacoustic microscopy in vivo with a 30-MHz ultrasound array transducer
    Roger J Zemp
    Washington University, Optical Imaging Laboratory, Department of Biomedical Engineering, Saint Louis, Missouri 63130, USA
    Opt Express 16:7915-28. 2008
    ..Realtime imaging performance is demonstrated on phantoms and in vivo with absorbing structures identified to depths of 2.5-3 mm. This work represents the first high-frequency realtime photoacoustic imaging system to our knowledge...
  29. ncbi request reprint Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography
    Xueding Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, Texas 77843 3120, USA
    J Biomed Opt 11:024015. 2006
    ..This technique, with its prominent sensitivity to endogenous contrast, can potentially contribute to the understanding of the interrelationship between neural, hemodynamic, and metabolic activities in the brain...
  30. ncbi request reprint Fiber-based polarization-sensitive Mueller matrix optical coherence tomography with continuous source polarization modulation
    Shuliang Jiao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Appl Opt 44:5463-7. 2005
    ..The system was successfully tested by imaging both standard polarization elements and biological samples...
  31. ncbi request reprint Multiple-bandwidth photoacoustic tomography
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843 3120, USA
    Phys Med Biol 49:1329-38. 2004
    ..The higher frequency detectors provided better image resolution while the lower frequency detectors delineated the major structural traits with a higher signal-noise ratio...
  32. pmc A 2.5-mm diameter probe for photoacoustic and ultrasonic endoscopy
    Joon Mo Yang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130, USA
    Opt Express 20:23944-53. 2012
    ....
  33. pmc Multi-scale molecular photoacoustic tomography of gene expression
    Xin Cai
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri, United States of America
    PLoS ONE 7:e43999. 2012
    ..These results suggest that PAT is a complementary tool to conventional optical fluorescence imaging of reporter genes for linking biological studies from the microscopic to the macroscopic scales...
  34. pmc Ultrasound-array-based real-time photoacoustic microscopy of human pulsatile dynamics in vivo
    Liang Song
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 15:021303. 2010
    ..Our results show that UA-PAM can potentially enable many new possibilities for studying functional and physiological dynamics in both preclinical and clinical imaging settings...
  35. ncbi request reprint Effects of acoustic heterogeneity in breast thermoacoustic tomography
    Yuan Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, USA
    IEEE Trans Ultrason Ferroelectr Freq Control 50:1134-46. 2003
    ..An approach to implement the compensation using only TAT data is proposed. Lastly, the differences in the effects of acoustic heterogeneity and the generation of speckles in breast TAT and breast ultrasound imaging are discussed...
  36. pmc Handheld array-based photoacoustic probe for guiding needle biopsy of sentinel lymph nodes
    Chulhong Kim
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri 63130 4899, USA
    J Biomed Opt 15:046010. 2010
    ..These results demonstrate the clinical potential of this handheld photoacoustic system for facile identification and needle biopsy of sentinel lymph nodes for cancer staging and metastasis detection in humans...
  37. pmc Performance benchmarks of an array-based hand-held photoacoustic probe adapted from a clinical ultrasound system for non-invasive sentinel lymph node imaging
    Chulhong Kim
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, MO 63130 4899, USA
    Philos Trans A Math Phys Eng Sci 369:4644-50. 2011
    ..2 cm depth) were 17 and 85 μM, ex vivo and in vivo, respectively. These results support the use of this PA system for non-invasive mapping and image-guided needle biopsy of sentinel nodes in breast cancer patients...
  38. ncbi request reprint Multiple-source optical diffusion approximation for a multilayer scattering medium
    Joseph L Hollmann
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Appl Opt 46:6004-9. 2007
    ..This multisource method is found to be significantly more accurate than the previous single-source method...
  39. ncbi request reprint Rhesus monkey brain imaging through intact skull with thermoacoustic tomography
    Yuan Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, USA
    IEEE Trans Ultrason Ferroelectr Freq Control 53:542-8. 2006
    ..Brain features that are 3 cm deep in the head are imaged clearly. Our results demonstrate that TAT has potential for use in portable, cost-effective imagers for pediatric brains...
  40. pmc Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array
    Liang Song
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 13:054028. 2008
    ..Fast 3-D photoacoustic microscopy is anticipated to facilitate applications of photoacoustic imaging in biomedical studies that involve dynamics and clinical procedures that demand immediate diagnosis...
  41. pmc High-resolution photoacoustic tomography of resting-state functional connectivity in the mouse brain
    Mohammadreza Nasiriavanaki
    Optical Imaging Laboratory, Department of Biomedical Engineering and Department of Radiology, Washington University in St Louis, St Louis, MO 63130
    Proc Natl Acad Sci U S A 111:21-6. 2014
    ..In addition to connectivity images, vascular images were simultaneously acquired. These studies show that functional connectivity photoacoustic tomography is a promising, noninvasive technique for functional imaging of the mouse brain. ..
  42. pmc Investigation of neovascularization in three-dimensional porous scaffolds in vivo by a combination of multiscale photoacoustic microscopy and optical coherence tomography
    Xin Cai
    Department of Biomedical Engineering, Washington University in St Louis, Missouri, USA
    Tissue Eng Part C Methods 19:196-204. 2013
    ..80 μm) of a scaffold on neovascularization. The data collected from PAM were consistent with those obtained from traditional invasive, labor-intensive histologic analyses...
  43. pmc Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror
    Junjie Yao
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, MO 63130, USA
    J Biomed Opt 17:080505-1. 2012
    ..Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena...
  44. pmc Immediate alterations in intestinal oxygen saturation and blood flow after massive small bowel resection as measured by photoacoustic microscopy
    Kathryn J Rowland
    Division of Pediatric Surgery, St Louis Children s Hospital, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    J Pediatr Surg 47:1143-9. 2012
    ..We sought to determine the effect of SBR on intestinal hemodynamics using photoacoustic microscopy, a noninvasive, label-free, high-resolution in vivo hybrid imaging modality...
  45. ncbi request reprint Photoacoustic tomography of biological tissues with high cross-section resolution: reconstruction and experiment
    Xueding Wang
    Optical Imaging Laboratory, Biomedical Engineering Program, Texas A and M University 3120 TAMU, College Station, Texas 77843 3120, USA
    Med Phys 29:2799-805. 2002
    ....
  46. doi request reprint Sentinel lymph node detection ex vivo using ultrasound-modulated optical tomography
    Chulhong Kim
    Washington University in Saint Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, Campus Box 1097, One Brookings Drive, Saint Louis, Missouri 63130 4899, USA
    J Biomed Opt 13:020507. 2008
    ....
  47. pmc Photoacoustic tomography of small animal brain with a curved array transducer
    Xinmai Yang
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130, USA
    J Biomed Opt 14:054007. 2009
    ..Last, ultrasonic gel coupling, instead of the previously adopted water coupling, is conveniently used in near-real-time 2-D imaging...
  48. pmc On the speckle-free nature of photoacoustic tomography
    Zijian Guo
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University, 1 Brookings Drive, Saint Louis, Missouri 63130, USA
    Med Phys 36:4084-8. 2009
    ..A long-standing conundrum is why photoacoustic tomography (PAT) possesses the unique ability to produce images devoid of speckle artifacts while all other coherent imaging technologies do not...
  49. pmc Single-walled carbon nanotubes as a multimodal-thermoacoustic and photoacoustic-contrast agent
    Manojit Pramanik
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 14:034018. 2009
    ..The large contrast enhancement of single-walled carbon nanotubes was further corroborated by tissue phantom imaging studies...
  50. ncbi request reprint Ultrasound-modulated optical tomography with intense acoustic bursts
    Roger J Zemp
    Otical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, Texas 77843 3120, USA
    Appl Opt 46:1615-23. 2007
    ..Additionally, we maintain high-resolution images representative of optical and not mechanical contrast. Signal-to-noise levels are sufficiently high so as to enable acquisition of 2D images of phantoms with one acoustic burst per pixel...
  51. pmc Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous scaffolds
    Yu Zhang
    Department of Biomedical Engineering, Washington University, One Brookings Drive, St Louis, MO 63130, USA
    Biomaterials 31:8651-8. 2010
    ..The cell proliferation profile obtained from PAM correlated well with what was obtained using the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
  52. pmc Ultrasound-modulated optical tomography at new depth
    Puxiang Lai
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 17:066006. 2012
    ..Within the safety limits for both laser illumination and ultrasound modulation, the system has attained the ability to image through a tissue-mimicking phantom of 9.4 cm in thickness, which has never been reached previously by UOT...
  53. pmc Single-cell label-free photoacoustic flowoxigraphy in vivo
    Lidai Wang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 110:5759-64. 2013
    ..The single-RBC functional imaging capability of FOG enables numerous biomedical studies and clinical applications...
  54. pmc Integrated optical- and acoustic-resolution photoacoustic microscopy based on an optical fiber bundle
    Wenxin Xing
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, One Brookings Drive, St Louis, Missouri 63130, USA
    Opt Lett 38:52-4. 2013
    ..2 and 40 μm as the maximum imaging depth is switched between 1.3 and 3.0 mm. The system enables automatically coregistered higher-resolution OR and deeper AR photoacoustic imaging...
  55. pmc Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo
    Junjie Yao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Neuroimage 64:257-66. 2013
    ..Our results demonstrate that 2-NBDG-enhanced PACT is a promising tool for noninvasive studies of brain metabolism...
  56. pmc Photoacoustic computed tomography correcting for heterogeneity and attenuation
    Chao Huang
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 17:061211. 2012
    ..Experiments with phantom objects are presented to validated our findings...
  57. pmc Spectrally encoded photoacoustic microscopy using a digital mirror device
    Yu Wang
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 17:066020. 2012
    ..Compared with external wavelength scanning of a wide-band laser with the same pulse energy, spectral encoding improves the signal-to-noise ratio...
  58. pmc Aberration correction for transcranial photoacoustic tomography of primates employing adjunct image data
    Chao Huang
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri, USA
    J Biomed Opt 17:066016. 2012
    ..The results establish that our reconstruction methodology can effectively compensate for skull-induced acoustic aberrations and improve image fidelity in transcranial PAT...
  59. pmc Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system
    Haixin Ke
    Washington University, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, MO, USA
    J Biomed Opt 17:056010. 2012
    ..5 mm, respectively, suitable for breast cancer imaging. A PA noise-equivalent sensitivity to methylene blue solution of 260 nM was achieved in chicken tissue at a depth of 3.4 cm...
  60. pmc Multiscale photoacoustic microscopy of single-walled carbon nanotube-incorporated tissue engineering scaffolds
    Xin Cai
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Tissue Eng Part C Methods 18:310-7. 2012
    ..Our results suggest that PAM is a promising tool for noninvasive real-time imaging and monitoring of tissue engineering scaffolds in vitro, and in vivo under physiological conditions...
  61. pmc Three-dimensional photoacoustic tomography based on the focal-line concept
    Jun Xia
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 16:090505. 2011
    ..The effectiveness of the proposed algorithm was first validated by numerical simulations and then demonstrated with a hair phantom experiment and an ex vivo mouse embryo experiment...
  62. ncbi request reprint Ex vivo blood vessel imaging using ultrasound-modulated optical microscopy
    Sri Rajasekhar Kothapalli
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130, USA
    J Biomed Opt 14:014015. 2009
    ..Therefore, UOM could be a promising tool to study the morphology of blood vasculature and blood-associated functional parameters, such as oxygen saturation...
  63. pmc High-speed dynamic 3D photoacoustic imaging of sentinel lymph node in a murine model using an ultrasound array
    Liang Song
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St Louis, St Louis, Missouri 63130, USA
    Med Phys 36:3724-9. 2009
    ..The demonstrated capability suggests that high-speed 3D photoacoustic imaging should facilitate the understanding of the dynamics of various dyes in SLNs and potentially help identify SLNs with high accuracy...
  64. pmc Fast voice-coil scanning optical-resolution photoacoustic microscopy
    Lidai Wang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130 4899, USA
    Opt Lett 36:139-41. 2011
    ....
  65. pmc Gold nanocages covered with thermally-responsive polymers for controlled release by high-intensity focused ultrasound
    Weiyang Li
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    Nanoscale 3:1724-30. 2011
    ..Because it can penetrate more deeply into soft tissues than near-infrared light, HIFU is a potentially more effective external stimulus for rapid, on-demand drug release...
  66. pmc Section-illumination photoacoustic microscopy for dynamic 3D imaging of microcirculation in vivo
    Liang Song
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA
    Opt Lett 35:1482-4. 2010
    ..5 Hz 3D image acquisition rates. Such observation allowed us to differentiate the arterioles from the venules. In the future, the technology may be used to study angiogenesis, diabetes-induced vascular complications, and pharmacokinetics...
  67. pmc Photoacoustic tomography of foreign bodies in soft biological tissue
    Xin Cai
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University, St Louis, Missouri 63130, USA
    J Biomed Opt 16:046017. 2011
    ..Spectroscopic PA imaging was also performed on the objects. These results suggest that PA imaging can potentially be a useful intraoperative imaging tool to identify foreign bodies...
  68. pmc In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure
    Yu Wang
    Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130 4899, USA
    Opt Lett 36:1029-31. 2011
    ..In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength...
  69. pmc Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue
    Zijian Guo
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University, 1 Brookings Drive, Saint Louis, Missouri 63130, USA
    Opt Lett 35:2067-9. 2010
    ..We also quantified both the concentration and oxygen saturation of hemoglobin in a live mouse in absolute units...
  70. ncbi request reprint Exact frequency-domain reconstruction for thermoacoustic tomography--I: Planar geometry
    Yuan Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843 3120, USA
    IEEE Trans Med Imaging 21:823-8. 2002
    ..We also demonstrate that the blurring caused by the finite size of the detector surface is the primary limiting factor on the resolution and that it can be compensated for by deconvolution...
  71. ncbi request reprint Imaging acute thermal burns by photoacoustic microscopy
    Hao F Zhang
    Department of Biomedical Engineering, Optical Imaging Laboratory, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    J Biomed Opt 11:054033. 2006
    ..73+/-0.07 mm. In burns treated at 150 degrees C for 5, 10, 20, and 30 s, respectively, the trend of increasing maximum burn depth with longer thermal exposure is demonstrated...
  72. ncbi request reprint Improved in vivo photoacoustic microscopy based on a virtual-detector concept
    Meng Lin Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Opt Lett 31:474-6. 2006
    ..In vivo experiments show that the technique also provides a clearer representation of the vascular distribution in the rat's scalp...
  73. ncbi request reprint Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Appl Opt 44:770-5. 2005
    ..This research demonstrates that PAT can potentially provide a powerful tool for small-animal biological research...
  74. pmc Design and evaluation of a novel breast cancer detection system combining both thermoacoustic (TA) and photoacoustic (PA) tomography
    Manojit Pramanik
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA
    Med Phys 35:2218-23. 2008
    ..Here we present the design of the breast scanner along with initial tissue phantom study results as a precursor to an actual patient study...
  75. ncbi request reprint Universal back-projection algorithm for photoacoustic computed tomography
    Minghua Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 71:016706. 2005
    ..A method for implementing this algorithm is described. Numerical simulation is used to demonstrate the performance of the algorithm...
  76. ncbi request reprint Determination of local polarization properties of biological samples in the presence of diattenuation by use of Mueller optical coherence tomography
    Milos Todorović
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843 3120, USA
    Opt Lett 29:2402-4. 2004
    ..We experimentally verify the algorithm using measurements of a section of porcine tendon and the septum of a rat heart...
  77. ncbi request reprint Transmission- and side-detection configurations in ultrasound-modulated optical tomography of thick biological tissues
    Jun Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Appl Opt 42:4088-94. 2003
    ..Experimental results implied feasibility of applying the ultrasound-modulation technique to characterize optical properties in inhomogeneous biological tissues...
  78. ncbi request reprint Time-domain reconstruction algorithms and numerical simulations for thermoacoustic tomography in various geometries
    Minghua Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, USA
    IEEE Trans Biomed Eng 50:1086-99. 2003
    ..We refer to these reconstruction formulas as modified back projections. Numerical results are given to illustrate the validity of these algorithms...
  79. ncbi request reprint Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels
    Mathangi Sivaramakrishnan
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University 3120 TAMU, College Station, TX 77843 3120, USA
    Phys Med Biol 52:1349-61. 2007
    ....
  80. ncbi request reprint Polarized light propagation through scattering media: time-resolved Monte Carlo simulations and experiments
    Xueding Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, College Station, Texas 77843 3120, USA
    J Biomed Opt 8:608-17. 2003
    ..A linear relationship between the average order of the scatters and the light propagation time can be used to explain the exponential decay of the degree of polarization of transmitted light...
  81. pmc Tumor glucose metabolism imaged in vivo in small animals with whole-body photoacoustic computed tomography
    Muhammad Rameez Chatni
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 17:076012. 2012
    ..Anatomy was imaged with the endogenous hemoglobin contrast, and glucose metabolism was imaged with a near-infrared dye-labeled 2-deoxyglucose...
  82. ncbi request reprint Deeply penetrating photoacoustic tomography in biological tissues enhanced with an optical contrast agent
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, 3120 TAMU, Texas A and M University, College Station, Texas 77843 3120, USA
    Opt Lett 30:507-9. 2005
    ..The resolution was found to deteriorate slowly with increasing imaging depth. The effects of detection bandwidth on the quality of images acquired simultaneously by four different ultrasonic transducers are described...
  83. ncbi request reprint Three-dimensional laser-induced photoacoustic tomography of mouse brain with the skin and skull intact
    Xueding Wang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843 3120, USA
    Opt Lett 28:1739-41. 2003
    ..The spatial resolution is primarily diffraction limited by the received photoacoustic waves. Imaged structures of the brain at different depths match the corresponding histological pictures well...
  84. pmc Label-free oxygen-metabolic photoacoustic microscopy in vivo
    Junjie Yao
    Washington University in St Louis, Department of Biomedical Engineering, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 16:076003. 2011
    ..Counterintuitively, increased MRO(2) does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism...
  85. pmc A new theranostic system based on gold nanocages and phase-change materials with unique features for photoacoustic imaging and controlled release
    Geon Dae Moon
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    J Am Chem Soc 133:4762-5. 2011
    ..We can control the release profile by varying the power of HIFU, the duration of exposure to HIFU, or both...
  86. ncbi request reprint Contrast mechanisms in polarization-sensitive Mueller-matrix optical coherence tomography and application in burn imaging
    Shuliang Jiao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Appl Opt 42:5191-7. 2003
    ..Thus, Mueller OCT has significant potential for application in the noninvasive assessment of burn depth...
  87. pmc Photoacoustic tomography of water in phantoms and tissue
    Zhun Xu
    Washington University, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130 4899, USA
    J Biomed Opt 15:036019. 2010
    ..The significant difference in photoacoustic signals between water and fat tissue indicates that the laser-based PAT has the potential to detect water content in tissue...
  88. pmc Three-dimensional combined photoacoustic and optical coherence microscopy for in vivo microcirculation studies
    Li Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, 1 Brookings Drive, Campus Box 1097, St Louis, Missouri 63130, USA
    Opt Express 17:16450-5. 2009
    ..Three-dimensional imaging of microvasculature and its local environment has been demonstrated at micrometer-order resolution using endogenous contrast in vivo...
  89. pmc Detection of ultrasound-modulated diffuse photons using spectral-hole burning
    Youzhi Li
    Biomedical Engineering Department, Washington University in St Louis, St Louis, MO 63130, USA
    Opt Express 16:14862-74. 2008
    ..It is also immune to speckle decorrelation, potentially allowing real-time in vivo imaging. Both theory and experiments are presented...
  90. pmc Tangential resolution improvement in thermoacoustic and photoacoustic tomography using a negative acoustic lens
    Manojit Pramanik
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 14:024028. 2009
    ..We then quantified the tangential resolution improvement using phantom images. We also showed that the use of the negative lens preserves the shape of an object after the image is reconstructed...
  91. pmc Noninvasive label-free imaging of microhemodynamics by optical-resolution photoacoustic microscopy
    Song Hu
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    Opt Express 17:7688-93. 2009
    ..OR-PAM enables functional volumetric imaging of the intact microcirculation, thereby providing greatly improved accuracy and versatility for broad biological and clinical applications...
  92. doi request reprint Ultrasound-modulated optical microscopy
    Sri Rajasekhar Kothapalli
    Washington University at St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130, USA
    J Biomed Opt 13:054046. 2008
    ..In addition, we use analytical calculations to show that modulation depth decreases with increasing ultrasound frequency...
  93. ncbi request reprint Analytic explanation of spatial resolution related to bandwidth and detector aperture size in thermoacoustic or photoacoustic reconstruction
    Minghua Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843 3120, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 67:056605. 2003
    ..The bandwidth affects both axial and lateral resolutions; in contrast, the detector aperture blurs the lateral resolution greatly but the axial resolution only slightly...
  94. ncbi request reprint Pulsed-microwave-induced thermoacoustic tomography: filtered backprojection in a circular measurement configuration
    Minghua Xu
    Optical Imaging Laboratory, Texas A and M University, College Station 77843 3120, USA
    Med Phys 29:1661-9. 2002
    ....
  95. ncbi request reprint Photorefractive detection of tissue optical and mechanical properties by ultrasound modulated optical tomography
    Xiao Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843, USA
    Opt Lett 32:656-8. 2007
    ....
  96. ncbi request reprint Time-domain reconstruction for thermoacoustic tomography in a spherical geometry
    Minghua Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843 3120, USA
    IEEE Trans Med Imaging 21:814-22. 2002
    ..Experiments demonstrate that the reconstructed images agree well with the original samples. The spatial resolution of the system reaches 0.5 mm...
  97. ncbi request reprint Reconstructions in limited-view thermoacoustic tomography
    Yuan Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Med Phys 31:724-33. 2004
    ..Computations are conducted for both numerically simulated and experimental data. The reconstructions confirm our theoretical predictions...
  98. pmc Thermoacoustic and photoacoustic sensing of temperature
    Manojit Pramanik
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 14:054024. 2009
    ..The deep-tissue imaging capability of this technique can potentially lead us to in vivo temperature monitoring in thermal or cryogenic applications...
  99. pmc Negative lens concept for photoacoustic tomography
    Changhui Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 78:021901. 2008
    ..Phantom experiments demonstrate that use of this concept greatly increases the detection region for PAT with high sensitivity...
  100. pmc Ultrasound-mediated biophotonic imaging: a review of acousto-optical tomography and photo-acoustic tomography
    Lihong V Wang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843 3120, USA
    Dis Markers 19:123-38. 2003
    ..Potential clinical applications of ultrasound-mediated biophotonic imaging include early cancer detection, functional imaging, and molecular imaging...

Research Grants12

  1. Melanoma detection by optical spectroscopy
    Lihong Wang; Fiscal Year: 2007
    ..1. To improve instrumentation. 2. To establish a statistically significant database. 3. To develop and validate a diagnostic algorithm. 4. To identify the pathophysiologic parameters responsible for the diagnostic optical features. ..
  2. Functional brain imaging by laser-induced PAT
    Lihong Wang; Fiscal Year: 2006
    ..abstract_text> ..
  3. Photons plus Ultrasound: Imaging and Sensing
    Lihong Wang; Fiscal Year: 2006
    ..Previous critiques have been answered particularly as they addressed the participation of women and mechanisms to attract and reward students. ..
  4. Full polarization characterization by OCT
    Lihong Wang; Fiscal Year: 2006
    ..Analyze and characterize the temporal healing process of burns in the animal model. ..
  5. Functional brain imaging by laser-induced PAT
    Lihong Wang; Fiscal Year: 2007
    ..abstract_text> ..
  6. High-resolution ultrasound-encoded optical tomography
    Lihong Wang; Fiscal Year: 2007
    ..The proposed technology--a quantum leap from the state of the art--is complementary to confocal microscopy and optical-coherence tomography and has the potential for broad application in biomedicine. ..
  7. High-frequency multi-modality imaging:photoacoustic
    Lihong Wang; Fiscal Year: 2005
    ..Aim 3. Characterize the imaging capability of the proposed hand-held probe by imaging atherosclerotic plaques ex vivo in a rabbit model (animal model 2). ..