Lihong V Wang

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi In vivo photoacoustic tomography of total blood flow and potential imaging of cancer angiogenesis and hypermetabolism
    Junjie Yao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Technol Cancer Res Treat 11:301-7. 2012
  2. ncbi Boundary conditions in photoacoustic tomography and image reconstruction
    Lihong V Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, Texas 77843 3120, USA
    J Biomed Opt 12:014027. 2007
  3. ncbi Prospects of photoacoustic tomography
    Lihong V Wang
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130 4899, USA
    Med Phys 35:5758-67. 2008
  4. ncbi Exact frequency-domain reconstruction for thermoacoustic tomography--II: Cylindrical geometry
    Yuan Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843-3120, USA
    IEEE Trans Med Imaging 21:829-33. 2002
  5. ncbi Effects of acoustic heterogeneities on transcranial brain imaging with microwave-induced thermoacoustic tomography
    Xing Jin
    Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Med Phys 35:3205-14. 2008
  6. ncbi Imaging of high-intensity focused ultrasound-induced lesions in soft biological tissue using thermoacoustic tomography
    Xing Jin
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843-3120, USA
    Med Phys 32:5-11. 2005
  7. ncbi Thermoacoustic tomography with correction for acoustic speed variations
    Xing Jin
    Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843-3120, USA
    Phys Med Biol 51:6437-48. 2006
  8. ncbi In vivo burn imaging using Mueller optical coherence tomography
    Milos Todorović
    Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, USA
    Opt Express 16:10279-84. 2008
  9. ncbi Image distortion in thermoacoustic tomography caused by microwave diffraction
    Changhui Li
    Biomedical Engineering Department, Washington University in St Louis, St Louis, Missouri 63130, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 77:031923. 2008
  10. ncbi In vivo molecular photoacoustic tomography of melanomas targeted by bioconjugated gold nanocages
    Chulhong Kim
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    ACS Nano 4:4559-64. 2010

Research Grants

  1. Melanoma detection by optical spectroscopy
    Lihong Wang; Fiscal Year: 2007
  2. Photons plus Ultrasound: Imaging and Sensing
    Lihong Wang; Fiscal Year: 2006
  3. Full polarization characterization by OCT
    Lihong Wang; Fiscal Year: 2006
  4. Functional brain imaging by laser-induced PAT
    Lihong Wang; Fiscal Year: 2007
  5. High-resolution ultrasound-encoded optical tomography
    Lihong Wang; Fiscal Year: 2007
  6. High-frequency multi-modality imaging:photoacoustic
    Lihong Wang; Fiscal Year: 2005

Collaborators

Detail Information

Publications113 found, 100 shown here

  1. ncbi In vivo photoacoustic tomography of total blood flow and potential imaging of cancer angiogenesis and hypermetabolism
    Junjie Yao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Technol Cancer Res Treat 11:301-7. 2012
    ..In addition, the method was tested in vivo on a mouse ear. We believe that the proposed method has the potential to be used for cancer angiogenesis and hypermetabolism imaging...
  2. ncbi Boundary conditions in photoacoustic tomography and image reconstruction
    Lihong V Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, Texas 77843 3120, USA
    J Biomed Opt 12:014027. 2007
    ..Analogously to the method of images of sources, which is commonly used in forward problems, the ultrasonic detectors are imaged about the boundary to satisfy the boundary condition in the inverse problem...
  3. ncbi Prospects of photoacoustic tomography
    Lihong V Wang
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130 4899, USA
    Med Phys 35:5758-67. 2008
    ....
  4. ncbi Exact frequency-domain reconstruction for thermoacoustic tomography--II: Cylindrical geometry
    Yuan Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843-3120, USA
    IEEE Trans Med Imaging 21:829-33. 2002
    ..The effect of discrete spatial sampling on image quality is analyzed. An aliasing-proof reconstruction method is proposed. Numerical and experimental results are included...
  5. ncbi Effects of acoustic heterogeneities on transcranial brain imaging with microwave-induced thermoacoustic tomography
    Xing Jin
    Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Med Phys 35:3205-14. 2008
    ..This work is important for obtaining good brain images when the thickness of the skull cannot be ignored...
  6. ncbi Imaging of high-intensity focused ultrasound-induced lesions in soft biological tissue using thermoacoustic tomography
    Xing Jin
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843-3120, USA
    Med Phys 32:5-11. 2005
    ..Gross pathologic photographs of the tissue samples confirmed the TAT images.This work demonstrates that TAT may potentially be used to image HIFU-induced lesions in biological tissues...
  7. ncbi Thermoacoustic tomography with correction for acoustic speed variations
    Xing Jin
    Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843-3120, USA
    Phys Med Biol 51:6437-48. 2006
    ..In this paper we develop a correction method based on ultrasonic transmission tomography (UTT) to improve the image quality of TAT. Numerical simulations and phantom experiments verify the effectiveness of this correction method...
  8. ncbi In vivo burn imaging using Mueller optical coherence tomography
    Milos Todorović
    Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, USA
    Opt Express 16:10279-84. 2008
    ..By using a decomposition algorithm developed by our group, we also mapped the local birefringence of the skin. The experimental results demonstrate the system's potential for in vivo burn-depth determination...
  9. ncbi Image distortion in thermoacoustic tomography caused by microwave diffraction
    Changhui Li
    Biomedical Engineering Department, Washington University in St Louis, St Louis, Missouri 63130, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 77:031923. 2008
    ..Both numerical simulations and phantom experiments demonstrate this phenomenon. A method of partial correction is also provided...
  10. ncbi In vivo molecular photoacoustic tomography of melanomas targeted by bioconjugated gold nanocages
    Chulhong Kim
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    ACS Nano 4:4559-64. 2010
    ..The in vivo PAT quantification of the amount of AuNCs accumulated in melanomas was further validated with inductively coupled plasma mass spectrometry (ICP-MS)...
  11. ncbi Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy
    Jung-Taek Oh
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, 77843-3120, USA
    J Biomed Opt 11:34032. 2006
    ..The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning...
  12. ncbi Quantitative photoacoustic microscopy of optical absorption coefficients from acoustic spectra in the optical diffusive regime
    Zijian Guo
    Washington University, Department of Biomedical Engineering, Optical Imaging Laboratory, 1 Brookings Drive, Saint Louis, Missouri 63130, USA
    J Biomed Opt 17:066011. 2012
    ..Compared with the conventional amplitude method, the acoustic spectral method provides greater quantification accuracy in the optical diffusive regime. The limitations of the acoustic spectral method was also discussed...
  13. ncbi In vivo three-dimensional photoacoustic imaging based on a clinical matrix array ultrasound probe
    Yu Wang
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 17:061208. 2012
    ..These results highlight the clinical potential of 3-D PA imaging for identification of sentinel lymph nodes for cancer staging in humans...
  14. ncbi Multiple-bandwidth photoacoustic tomography
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843-3120, USA
    Phys Med Biol 49:1329-38. 2004
    ..The higher frequency detectors provided better image resolution while the lower frequency detectors delineated the major structural traits with a higher signal-noise ratio...
  15. ncbi Fiber-based polarization-sensitive Mueller matrix optical coherence tomography with continuous source polarization modulation
    Shuliang Jiao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Appl Opt 44:5463-7. 2005
    ..The system was successfully tested by imaging both standard polarization elements and biological samples...
  16. ncbi Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer
    Roger J Zemp
    Washington University, Optical Imaging Laboratory, Department of Biomedical Engineering, Saint Louis, Missouri 63130, USA
    J Biomed Opt 12:010501. 2007
    ..It is anticipated that the system can be used for studying and diagnosing a number of diseases including cancer, atherosclerosis, dermatological disorders, and peripheral microvascular complications in diabetes...
  17. ncbi Thermoacoustic and photoacoustic tomography of thick biological tissues toward breast imaging
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843-3120, USA
    Technol Cancer Res Treat 4:559-66. 2005
    ..Based on the intrinsic optical contrast between blood and chicken breast muscle, an embedded blood object that was 5 cm deep in the tissue was also detected using PAT at a wavelength of 1064 nm...
  18. ncbi Realtime photoacoustic microscopy in vivo with a 30-MHz ultrasound array transducer
    Roger J Zemp
    Washington University, Optical Imaging Laboratory, Department of Biomedical Engineering, Saint Louis, Missouri 63130, USA
    Opt Express 16:7915-28. 2008
    ..Realtime imaging performance is demonstrated on phantoms and in vivo with absorbing structures identified to depths of 2.5-3 mm. This work represents the first high-frequency realtime photoacoustic imaging system to our knowledge...
  19. ncbi Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography
    Xueding Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, 3120 TAMU, College Station, Texas 77843-3120, USA
    J Biomed Opt 11:024015. 2006
    ..This technique, with its prominent sensitivity to endogenous contrast, can potentially contribute to the understanding of the interrelationship between neural, hemodynamic, and metabolic activities in the brain...
  20. ncbi Multi-scale molecular photoacoustic tomography of gene expression
    Xin Cai
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri, United States of America
    PLoS ONE 7:e43999. 2012
    ..These results suggest that PAT is a complementary tool to conventional optical fluorescence imaging of reporter genes for linking biological studies from the microscopic to the macroscopic scales...
  21. ncbi Handheld array-based photoacoustic probe for guiding needle biopsy of sentinel lymph nodes
    Chulhong Kim
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri 63130 4899, USA
    J Biomed Opt 15:046010. 2010
    ..These results demonstrate the clinical potential of this handheld photoacoustic system for facile identification and needle biopsy of sentinel lymph nodes for cancer staging and metastasis detection in humans...
  22. ncbi Rhesus monkey brain imaging through intact skull with thermoacoustic tomography
    Yuan Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843-3120, USA
    IEEE Trans Ultrason Ferroelectr Freq Control 53:542-8. 2006
    ..Brain features that are 3 cm deep in the head are imaged clearly. Our results demonstrate that TAT has potential for use in portable, cost-effective imagers for pediatric brains...
  23. ncbi Half-time image reconstruction in thermoacoustic tomography
    Mark A Anastasio
    Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA
    IEEE Trans Med Imaging 24:199-210. 2005
    ..Reconstructed images and numerical results produced from simulated and experimental TAT measurement data are employed to demonstrate these effects...
  24. ncbi Real-time four-dimensional optical-resolution photoacoustic microscopy with Au nanoparticle-assisted subdiffraction-limit resolution
    Bin Rao
    Department of Biomedical Engineering, Optical Imaging Lab, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130 4899, USA
    Opt Lett 36:1137-9. 2011
    ..The corresponding 2D B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23 ± 0.03 μm for Au nanowire imaging, which is 2.0 times below the diffraction limit...
  25. ncbi Effects of acoustic heterogeneity in breast thermoacoustic tomography
    Yuan Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843-3120, USA
    IEEE Trans Ultrason Ferroelectr Freq Control 50:1134-46. 2003
    ..An approach to implement the compensation using only TAT data is proposed. Lastly, the differences in the effects of acoustic heterogeneity and the generation of speckles in breast TAT and breast ultrasound imaging are discussed...
  26. ncbi Fast 3-D dark-field reflection-mode photoacoustic microscopy in vivo with a 30-MHz ultrasound linear array
    Liang Song
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 13:054028. 2008
    ..Fast 3-D photoacoustic microscopy is anticipated to facilitate applications of photoacoustic imaging in biomedical studies that involve dynamics and clinical procedures that demand immediate diagnosis...
  27. ncbi Performance benchmarks of an array-based hand-held photoacoustic probe adapted from a clinical ultrasound system for non-invasive sentinel lymph node imaging
    Chulhong Kim
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, MO 63130 4899, USA
    Philos Trans A Math Phys Eng Sci 369:4644-50. 2011
    ..2 cm depth) were 17 and 85 μM, ex vivo and in vivo, respectively. These results support the use of this PA system for non-invasive mapping and image-guided needle biopsy of sentinel nodes in breast cancer patients...
  28. ncbi Multiple-source optical diffusion approximation for a multilayer scattering medium
    Joseph L Hollmann
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Appl Opt 46:6004-9. 2007
    ..This multisource method is found to be significantly more accurate than the previous single-source method...
  29. ncbi Ultrasound-array-based real-time photoacoustic microscopy of human pulsatile dynamics in vivo
    Liang Song
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 15:021303. 2010
    ..Our results show that UA-PAM can potentially enable many new possibilities for studying functional and physiological dynamics in both preclinical and clinical imaging settings...
  30. ncbi Immediate alterations in intestinal oxygen saturation and blood flow after massive small bowel resection as measured by photoacoustic microscopy
    Kathryn J Rowland
    Division of Pediatric Surgery, St Louis Children s Hospital, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    J Pediatr Surg 47:1143-9. 2012
    ..We sought to determine the effect of SBR on intestinal hemodynamics using photoacoustic microscopy, a noninvasive, label-free, high-resolution in vivo hybrid imaging modality...
  31. ncbi Ultrasound-modulated optical tomography with intense acoustic bursts
    Roger J Zemp
    Otical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, Texas 77843 3120, USA
    Appl Opt 46:1615-23. 2007
    ..Additionally, we maintain high-resolution images representative of optical and not mechanical contrast. Signal-to-noise levels are sufficiently high so as to enable acquisition of 2D images of phantoms with one acoustic burst per pixel...
  32. ncbi Photoacoustic tomography of biological tissues with high cross-section resolution: reconstruction and experiment
    Xueding Wang
    Optical Imaging Laboratory, Biomedical Engineering Program, Texas A and M University 3120 TAMU, College Station, Texas 77843-3120, USA
    Med Phys 29:2799-805. 2002
    ....
  33. ncbi Single-walled carbon nanotubes as a multimodal-thermoacoustic and photoacoustic-contrast agent
    Manojit Pramanik
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 14:034018. 2009
    ..The large contrast enhancement of single-walled carbon nanotubes was further corroborated by tissue phantom imaging studies...
  34. ncbi Sentinel lymph node detection ex vivo using ultrasound-modulated optical tomography
    Chulhong Kim
    Washington University in Saint Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, Campus Box 1097, One Brookings Drive, Saint Louis, Missouri 63130 4899, USA
    J Biomed Opt 13:020507. 2008
    ....
  35. ncbi Photoacoustic tomography of small animal brain with a curved array transducer
    Xinmai Yang
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130, USA
    J Biomed Opt 14:054007. 2009
    ..Last, ultrasonic gel coupling, instead of the previously adopted water coupling, is conveniently used in near-real-time 2-D imaging...
  36. ncbi On the speckle-free nature of photoacoustic tomography
    Zijian Guo
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University, 1 Brookings Drive, Saint Louis, Missouri 63130, USA
    Med Phys 36:4084-8. 2009
    ..A long-standing conundrum is why photoacoustic tomography (PAT) possesses the unique ability to produce images devoid of speckle artifacts while all other coherent imaging technologies do not...
  37. ncbi Spectrally encoded photoacoustic microscopy using a digital mirror device
    Yu Wang
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 17:066020. 2012
    ..Compared with external wavelength scanning of a wide-band laser with the same pulse energy, spectral encoding improves the signal-to-noise ratio...
  38. ncbi Aberration correction for transcranial photoacoustic tomography of primates employing adjunct image data
    Chao Huang
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri, USA
    J Biomed Opt 17:066016. 2012
    ..The results establish that our reconstruction methodology can effectively compensate for skull-induced acoustic aberrations and improve image fidelity in transcranial PAT...
  39. ncbi Photoacoustic computed tomography correcting for heterogeneity and attenuation
    Chao Huang
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 17:061211. 2012
    ..Experiments with phantom objects are presented to validated our findings...
  40. ncbi Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system
    Haixin Ke
    Washington University, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, MO, USA
    J Biomed Opt 17:056010. 2012
    ..5 mm, respectively, suitable for breast cancer imaging. A PA noise-equivalent sensitivity to methylene blue solution of 260 nM was achieved in chicken tissue at a depth of 3.4 cm...
  41. ncbi Multiscale photoacoustic microscopy of single-walled carbon nanotube-incorporated tissue engineering scaffolds
    Xin Cai
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Tissue Eng Part C Methods 18:310-7. 2012
    ..Our results suggest that PAM is a promising tool for noninvasive real-time imaging and monitoring of tissue engineering scaffolds in vitro, and in vivo under physiological conditions...
  42. ncbi Three-dimensional photoacoustic tomography based on the focal-line concept
    Jun Xia
    Washington University in St Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 16:090505. 2011
    ..The effectiveness of the proposed algorithm was first validated by numerical simulations and then demonstrated with a hair phantom experiment and an ex vivo mouse embryo experiment...
  43. ncbi Design and evaluation of a novel breast cancer detection system combining both thermoacoustic (TA) and photoacoustic (PA) tomography
    Manojit Pramanik
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA
    Med Phys 35:2218-23. 2008
    ..Here we present the design of the breast scanner along with initial tissue phantom study results as a precursor to an actual patient study...
  44. ncbi Imaging of tumor angiogenesis in rat brains in vivo by photoacoustic tomography
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843 3120, USA
    Appl Opt 44:770-5. 2005
    ..This research demonstrates that PAT can potentially provide a powerful tool for small-animal biological research...
  45. ncbi Section-illumination photoacoustic microscopy for dynamic 3D imaging of microcirculation in vivo
    Liang Song
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA
    Opt Lett 35:1482-4. 2010
    ..5 Hz 3D image acquisition rates. Such observation allowed us to differentiate the arterioles from the venules. In the future, the technology may be used to study angiogenesis, diabetes-induced vascular complications, and pharmacokinetics...
  46. ncbi High-speed dynamic 3D photoacoustic imaging of sentinel lymph node in a murine model using an ultrasound array
    Liang Song
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St Louis, St Louis, Missouri 63130, USA
    Med Phys 36:3724-9. 2009
    ..The demonstrated capability suggests that high-speed 3D photoacoustic imaging should facilitate the understanding of the dynamics of various dyes in SLNs and potentially help identify SLNs with high accuracy...
  47. ncbi Calibration-free absolute quantification of optical absorption coefficients using acoustic spectra in 3D photoacoustic microscopy of biological tissue
    Zijian Guo
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University, 1 Brookings Drive, Saint Louis, Missouri 63130, USA
    Opt Lett 35:2067-9. 2010
    ..We also quantified both the concentration and oxygen saturation of hemoglobin in a live mouse in absolute units...
  48. ncbi Limitations of quantitative photoacoustic measurements of blood oxygenation in small vessels
    Mathangi Sivaramakrishnan
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University 3120 TAMU, College Station, TX 77843 3120, USA
    Phys Med Biol 52:1349-61. 2007
    ....
  49. ncbi Exact frequency-domain reconstruction for thermoacoustic tomography--I: Planar geometry
    Yuan Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843-3120, USA
    IEEE Trans Med Imaging 21:823-8. 2002
    ..We also demonstrate that the blurring caused by the finite size of the detector surface is the primary limiting factor on the resolution and that it can be compensated for by deconvolution...
  50. ncbi Transmission- and side-detection configurations in ultrasound-modulated optical tomography of thick biological tissues
    Jun Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Appl Opt 42:4088-94. 2003
    ..Experimental results implied feasibility of applying the ultrasound-modulation technique to characterize optical properties in inhomogeneous biological tissues...
  51. ncbi Time-domain reconstruction algorithms and numerical simulations for thermoacoustic tomography in various geometries
    Minghua Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843-3120, USA
    IEEE Trans Biomed Eng 50:1086-99. 2003
    ..We refer to these reconstruction formulas as modified back projections. Numerical results are given to illustrate the validity of these algorithms...
  52. ncbi Polarized light propagation through scattering media: time-resolved Monte Carlo simulations and experiments
    Xueding Wang
    Texas A and M University, Department of Biomedical Engineering, Optical Imaging Laboratory, College Station, Texas 77843-3120, USA
    J Biomed Opt 8:608-17. 2003
    ..A linear relationship between the average order of the scatters and the light propagation time can be used to explain the exponential decay of the degree of polarization of transmitted light...
  53. ncbi Determination of local polarization properties of biological samples in the presence of diattenuation by use of Mueller optical coherence tomography
    Milos Todorović
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843 3120, USA
    Opt Lett 29:2402-4. 2004
    ..We experimentally verify the algorithm using measurements of a section of porcine tendon and the septum of a rat heart...
  54. ncbi Universal back-projection algorithm for photoacoustic computed tomography
    Minghua Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 71:016706. 2005
    ..A method for implementing this algorithm is described. Numerical simulation is used to demonstrate the performance of the algorithm...
  55. ncbi In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure
    Yu Wang
    Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130 4899, USA
    Opt Lett 36:1029-31. 2011
    ..In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength...
  56. ncbi Photoacoustic tomography of foreign bodies in soft biological tissue
    Xin Cai
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University, St Louis, Missouri 63130, USA
    J Biomed Opt 16:046017. 2011
    ..Spectroscopic PA imaging was also performed on the objects. These results suggest that PA imaging can potentially be a useful intraoperative imaging tool to identify foreign bodies...
  57. ncbi Imaging acute thermal burns by photoacoustic microscopy
    Hao F Zhang
    Department of Biomedical Engineering, Optical Imaging Laboratory, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    J Biomed Opt 11:054033. 2006
    ..73+/-0.07 mm. In burns treated at 150 degrees C for 5, 10, 20, and 30 s, respectively, the trend of increasing maximum burn depth with longer thermal exposure is demonstrated...
  58. ncbi Ex vivo blood vessel imaging using ultrasound-modulated optical microscopy
    Sri Rajasekhar Kothapalli
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130, USA
    J Biomed Opt 14:014015. 2009
    ..Therefore, UOM could be a promising tool to study the morphology of blood vasculature and blood-associated functional parameters, such as oxygen saturation...
  59. ncbi Improved in vivo photoacoustic microscopy based on a virtual-detector concept
    Meng-Lin Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Opt Lett 31:474-6. 2006
    ..In vivo experiments show that the technique also provides a clearer representation of the vascular distribution in the rat's scalp...
  60. ncbi Fast voice-coil scanning optical-resolution photoacoustic microscopy
    Lidai Wang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130 4899, USA
    Opt Lett 36:139-41. 2011
    ....
  61. ncbi Gold nanocages covered with thermally-responsive polymers for controlled release by high-intensity focused ultrasound
    Weiyang Li
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    Nanoscale 3:1724-30. 2011
    ..Because it can penetrate more deeply into soft tissues than near-infrared light, HIFU is a potentially more effective external stimulus for rapid, on-demand drug release...
  62. ncbi Tumor glucose metabolism imaged in vivo in small animals with whole-body photoacoustic computed tomography
    Muhammad Rameez Chatni
    Washington University in St Louis, Department of Biomedical Engineering, St Louis, Missouri 63130, USA
    J Biomed Opt 17:076012. 2012
    ..Anatomy was imaged with the endogenous hemoglobin contrast, and glucose metabolism was imaged with a near-infrared dye-labeled 2-deoxyglucose...
  63. ncbi Label-free oxygen-metabolic photoacoustic microscopy in vivo
    Junjie Yao
    Washington University in St Louis, Department of Biomedical Engineering, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 16:076003. 2011
    ..Counterintuitively, increased MRO(2) does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism...
  64. ncbi Noninvasive label-free imaging of microhemodynamics by optical-resolution photoacoustic microscopy
    Song Hu
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    Opt Express 17:7688-93. 2009
    ..OR-PAM enables functional volumetric imaging of the intact microcirculation, thereby providing greatly improved accuracy and versatility for broad biological and clinical applications...
  65. ncbi Tangential resolution improvement in thermoacoustic and photoacoustic tomography using a negative acoustic lens
    Manojit Pramanik
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 14:024028. 2009
    ..We then quantified the tangential resolution improvement using phantom images. We also showed that the use of the negative lens preserves the shape of an object after the image is reconstructed...
  66. ncbi Three-dimensional combined photoacoustic and optical coherence microscopy for in vivo microcirculation studies
    Li Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, 1 Brookings Drive, Campus Box 1097, St Louis, Missouri 63130, USA
    Opt Express 17:16450-5. 2009
    ..Three-dimensional imaging of microvasculature and its local environment has been demonstrated at micrometer-order resolution using endogenous contrast in vivo...
  67. ncbi Photoacoustic tomography of water in phantoms and tissue
    Zhun Xu
    Washington University, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130 4899, USA
    J Biomed Opt 15:036019. 2010
    ..The significant difference in photoacoustic signals between water and fat tissue indicates that the laser-based PAT has the potential to detect water content in tissue...
  68. ncbi Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous scaffolds
    Yu Zhang
    Department of Biomedical Engineering, Washington University, One Brookings Drive, St Louis, MO 63130, USA
    Biomaterials 31:8651-8. 2010
    ..The cell proliferation profile obtained from PAM correlated well with what was obtained using the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
  69. ncbi A new theranostic system based on gold nanocages and phase-change materials with unique features for photoacoustic imaging and controlled release
    Geon Dae Moon
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    J Am Chem Soc 133:4762-5. 2011
    ..We can control the release profile by varying the power of HIFU, the duration of exposure to HIFU, or both...
  70. ncbi Ultrasound-modulated optical microscopy
    Sri Rajasekhar Kothapalli
    Washington University at St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St Louis, Missouri 63130, USA
    J Biomed Opt 13:054046. 2008
    ..In addition, we use analytical calculations to show that modulation depth decreases with increasing ultrasound frequency...
  71. ncbi Deeply penetrating photoacoustic tomography in biological tissues enhanced with an optical contrast agent
    Geng Ku
    Optical Imaging Laboratory, Department of Biomedical Engineering, 3120 TAMU, Texas A and M University, College Station, Texas 77843-3120, USA
    Opt Lett 30:507-9. 2005
    ..The resolution was found to deteriorate slowly with increasing imaging depth. The effects of detection bandwidth on the quality of images acquired simultaneously by four different ultrasonic transducers are described...
  72. ncbi Three-dimensional laser-induced photoacoustic tomography of mouse brain with the skin and skull intact
    Xueding Wang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843-3120, USA
    Opt Lett 28:1739-41. 2003
    ..The spatial resolution is primarily diffraction limited by the received photoacoustic waves. Imaged structures of the brain at different depths match the corresponding histological pictures well...
  73. ncbi Detection of ultrasound-modulated diffuse photons using spectral-hole burning
    Youzhi Li
    Biomedical Engineering Department, Washington University in St Louis, St Louis, MO 63130, USA
    Opt Express 16:14862-74. 2008
    ..It is also immune to speckle decorrelation, potentially allowing real-time in vivo imaging. Both theory and experiments are presented...
  74. ncbi Contrast mechanisms in polarization-sensitive Mueller-matrix optical coherence tomography and application in burn imaging
    Shuliang Jiao
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Appl Opt 42:5191-7. 2003
    ..Thus, Mueller OCT has significant potential for application in the noninvasive assessment of burn depth...
  75. ncbi Analytic explanation of spatial resolution related to bandwidth and detector aperture size in thermoacoustic or photoacoustic reconstruction
    Minghua Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843-3120, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 67:056605. 2003
    ..The bandwidth affects both axial and lateral resolutions; in contrast, the detector aperture blurs the lateral resolution greatly but the axial resolution only slightly...
  76. ncbi Pulsed-microwave-induced thermoacoustic tomography: filtered backprojection in a circular measurement configuration
    Minghua Xu
    Optical Imaging Laboratory, Texas A and M University, College Station 77843-3120, USA
    Med Phys 29:1661-9. 2002
    ....
  77. ncbi Time-domain reconstruction for thermoacoustic tomography in a spherical geometry
    Minghua Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843-3120, USA
    IEEE Trans Med Imaging 21:814-22. 2002
    ..Experiments demonstrate that the reconstructed images agree well with the original samples. The spatial resolution of the system reaches 0.5 mm...
  78. ncbi Reconstructions in limited-view thermoacoustic tomography
    Yuan Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Med Phys 31:724-33. 2004
    ..Computations are conducted for both numerically simulated and experimental data. The reconstructions confirm our theoretical predictions...
  79. ncbi Photorefractive detection of tissue optical and mechanical properties by ultrasound modulated optical tomography
    Xiao Xu
    Department of Biomedical Engineering, Texas A and M University, College Station 77843, USA
    Opt Lett 32:656-8. 2007
    ....
  80. ncbi Ultrasound-mediated biophotonic imaging: a review of acousto-optical tomography and photo-acoustic tomography
    Lihong V Wang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, TX 77843 3120, USA
    Dis Markers 19:123-38. 2003
    ..Potential clinical applications of ultrasound-mediated biophotonic imaging include early cancer detection, functional imaging, and molecular imaging...
  81. ncbi Jones-matrix imaging of biological tissues with quadruple-channel optical coherence tomography
    Shuliang Jiao
    Texas A and M University, Optical Imaging Laboratory, Biomedical Engineering Program, College Station, Texas 77843-3120, USA
    J Biomed Opt 7:350-8. 2002
    ..Birefringence is sensitive to changes in tissue because it is based on phase contrast...
  82. ncbi Molecular photoacoustic imaging of angiogenesis with integrin-targeted gold nanobeacons
    Dipanjan Pan
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63108, USA
    FASEB J 25:875-82. 2011
    ....
  83. ncbi Gold nanostructures: a class of multifunctional materials for biomedical applications
    Claire M Cobley
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    Chem Soc Rev 40:44-56. 2011
    ....
  84. ncbi Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging
    Hao F Zhang
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Nat Biotechnol 24:848-51. 2006
    ..fPAM is demonstrated with in vivo imaging of angiogenesis, melanoma, hemoglobin oxygen saturation (sO2) of single vessels in animals and total hemoglobin concentration in humans...
  85. ncbi Photoacoustic tomography and sensing in biomedicine
    Changhui Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    Phys Med Biol 54:R59-97. 2009
    ..The emphasized areas include the new imaging modalities, hybrid detection methods, photoacoustic contrast agents and the photoacoustic Doppler effect, as well as translational research topics...
  86. ncbi Noninvasive in vivo spectroscopic nanorod-contrast photoacoustic mapping of sentinel lymph nodes
    Kwang Hyun Song
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130 4899, USA
    Eur J Radiol 70:227-31. 2009
    ..Gold nanorods have biocompatibility, high optical absorption, and easily tuned surface plasmon resonance peak wavelength...
  87. ncbi Negative lens concept for photoacoustic tomography
    Changhui Li
    Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 78:021901. 2008
    ..Phantom experiments demonstrate that use of this concept greatly increases the detection region for PAT with high sensitivity...
  88. ncbi Molecular photoacoustic tomography with colloidal nanobeacons
    Dipanjan Pan
    C TRAIN and Division of Cardiology, Washington University School of Medicine, 4320 Forest Park Avenue, Saint Louis, MO 63108, USA
    Angew Chem Int Ed Engl 48:4170-3. 2009
    ..Fibrin-targeted GNBs provide a more than tenfold signal enhancement in photoacoustic tomography in the near-IR wavelength window, indicating their potential for diagnostic imaging...
  89. ncbi Near infrared photoacoustic detection of sentinel lymph nodes with gold nanobeacons
    Dipanjan Pan
    C TRAIN and Division of Cardiology, Washington University School of Medicine, Saint Louis, MO 63108, USA
    Biomaterials 31:4088-93. 2010
    ....
  90. ncbi Thermoacoustic and photoacoustic sensing of temperature
    Manojit Pramanik
    Washington University in St Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 14:054024. 2009
    ..The deep-tissue imaging capability of this technique can potentially lead us to in vivo temperature monitoring in thermal or cryogenic applications...
  91. ncbi In vivo three-dimensional photoacoustic tomography of a whole mouse head
    Kwang Hyun Song
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843, USA
    Opt Lett 31:2453-5. 2006
    ..Deeply seated arterial and venous vessels in the head measuring up to 1.7 cm in diameter were simultaneously imaged in vivo with 804 nm wavelength laser excitation of photoacoustic waves...
  92. ncbi High-resolution ultrasound-modulated optical tomography in biological tissues
    Sava Sakadzic
    Optical Imaging Laboratory, Department of Biomedical Engineering, 3120 TAMU, Texas A and M University, College Station, Texas 77843 3120, USA
    Opt Lett 29:2770-2. 2004
    ..This technology is complementary to other imaging technologies, such as confocal microscopy and optical-coherence tomography, and has the potential for broad biomedical applications...
  93. ncbi Propagation of polarized light in birefringent turbid media: a Monte Carlo study
    Xueding Wang
    Texas A and M University, Optical Imaging Laboratory, Biomedical Engineering Program, 3120 TAMU, College Station, Texas 77843-3120, USA
    J Biomed Opt 7:279-90. 2002
    ....
  94. ncbi Time reversal and its application to tomography with diffracting sources
    Yuan Xu
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, 3120 TAMU, College Station, Texas 77843-3120, USA
    Phys Rev Lett 92:033902. 2004
    ..The algorithm is verified by a numerical simulation. Extension to the case using Green's function in a heterogeneous medium is discussed...
  95. ncbi Photoacoustic tomography of a rat cerebral cortex in vivo with au nanocages as an optical contrast agent
    Xinmai Yang
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Nano Lett 7:3798-802. 2007
    ..A gradual enhancement of the optical absorption in the cerebral cortex, by up to 81%, was observed over the course of the experiment...
  96. ncbi Intense acoustic bursts as a signal-enhancement mechanism in ultrasound-modulated optical tomography
    Chulhong Kim
    Optical Imaging Laboratory, Department of Biomedical Engineering, 3120 TAMU, Texas A and M University, College Station, TX 77843-3120, USA
    Opt Lett 31:2423-5. 2006
    ..Because of the substantially improved SNR, the use of intense acoustic bursts is a promising signal enhancement strategy for UOT...
  97. ncbi Compressed sensing in photoacoustic tomography in vivo
    Zijian Guo
    Washington University, Department of Biomedical Engineering, Optical Imaging Laboratory, 1 Brookings Drive, St Louis, Missouri 63130, USA
    J Biomed Opt 15:021311. 2010
    ..We adapt compressed sensing (CS) for the reconstruction in PACT. CS-based PACT is implemented as a nonlinear conjugate gradient descent algorithm and tested with both phantom and in vivo experiments...
  98. ncbi Correlation transfer equation for multiply scattered light modulated by an ultrasonic pulse
    Sava Sakadzic
    Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, USA
    J Opt Soc Am A Opt Image Sci Vis 24:2797-806. 2007
    ..We expect the CTE and MC to be applicable for estimation of contrast and resolution in a wide spectrum of conditions in ultrasound-modulated optical tomography of soft biological tissues...
  99. ncbi Degree of polarization in laser speckles from turbid media: implications in tissue optics
    Jun Li
    Texas A and M University, Optical Imaging Laboratory, Biomedical Engineering Program, 3120 TAMU, College Station, Texas 77843-3120, USA
    J Biomed Opt 7:307-12. 2002
    ..A numerical simulation explained the experimental observation. These results are important for the understanding of polarization phenomena in turbid media such as biological tissue...
  100. ncbi Imaging optically scattering objects with ultrasound-modulated optical tomography
    Sri Rajasekhar Kothapalli
    Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St Louis, One Brookings Drive, St Louis, MO 63130 4899, USA
    Opt Lett 32:2351-3. 2007
    ..This study complements previous UOT experiments that demonstrated optical absorption contrast...
  101. ncbi Stochastic explanation of speckle contrast detection in ultrasound-modulated optical tomography
    Roger Zemp
    Optical Imaging Laboratory, Department of Biomedical Engineering, Texas A and M University, College Station, Texas 77843-3120, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 73:061920. 2006
    ..The theory predicts that speckle contrast changes linearly with applied acoustic intensity. This prediction is experimentally validated for both 1 and ultrasound. Signal dependence on ultrasound frequency is discussed...

Research Grants12

  1. Melanoma detection by optical spectroscopy
    Lihong Wang; Fiscal Year: 2007
    ..1. To improve instrumentation. 2. To establish a statistically significant database. 3. To develop and validate a diagnostic algorithm. 4. To identify the pathophysiologic parameters responsible for the diagnostic optical features. ..
  2. Photons plus Ultrasound: Imaging and Sensing
    Lihong Wang; Fiscal Year: 2006
    ..Previous critiques have been answered particularly as they addressed the participation of women and mechanisms to attract and reward students. ..
  3. Full polarization characterization by OCT
    Lihong Wang; Fiscal Year: 2006
    ..Analyze and characterize the temporal healing process of burns in the animal model. ..
  4. Functional brain imaging by laser-induced PAT
    Lihong Wang; Fiscal Year: 2007
    ..abstract_text> ..
  5. High-resolution ultrasound-encoded optical tomography
    Lihong Wang; Fiscal Year: 2007
    ..The proposed technology--a quantum leap from the state of the art--is complementary to confocal microscopy and optical-coherence tomography and has the potential for broad application in biomedicine. ..
  6. High-frequency multi-modality imaging:photoacoustic
    Lihong Wang; Fiscal Year: 2005
    ..Aim 3. Characterize the imaging capability of the proposed hand-held probe by imaging atherosclerotic plaques ex vivo in a rabbit model (animal model 2). ..