Stavros Thomopoulos

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi request reprint The development of structural and mechanical anisotropy in fibroblast populated collagen gels
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University School of Medicine, 1 Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St, Louis, MO 63110, USA
    J Biomech Eng 127:742-50. 2005
  2. ncbi request reprint Effect of several growth factors on canine flexor tendon fibroblast proliferation and collagen synthesis in vitro
    Stavros Thomopoulos
    Department of Orthopaedic Research, Washington University, St Louis, MO, USA
    J Hand Surg Am 30:441-7. 2005
  3. ncbi request reprint Collagen fiber orientation at the tendon to bone insertion and its influence on stress concentrations
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, 1 Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St Louis, MO 63110, USA
    J Biomech 39:1842-51. 2006
  4. pmc Enhanced flexor tendon healing through controlled delivery of PDGF-BB
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri, USA
    J Orthop Res 27:1209-15. 2009
  5. pmc Controlled-release kinetics and biologic activity of platelet-derived growth factor-BB for use in flexor tendon repair
    Shelly E Sakiyama-Elbert
    Department of Orthopaedic Surgery, Washington University in St Louis, St Louis, MO, USA
    J Hand Surg Am 33:1548-57. 2008
  6. ncbi request reprint PDGF-BB released in tendon repair using a novel delivery system promotes cell proliferation and collagen remodeling
    Stavros Thomopoulos
    Department of Orthopaedics, Washington University, St Louis, Missouri 63110, USA
    J Orthop Res 25:1358-68. 2007
  7. pmc Use of a magnesium-based bone adhesive for flexor tendon-to-bone healing
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
    J Hand Surg Am 34:1066-73. 2009
  8. pmc The effects of exogenous basic fibroblast growth factor on intrasynovial flexor tendon healing in a canine model
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St Louis, MO 63110
    J Bone Joint Surg Am 92:2285-93. 2010
  9. pmc The tendon-to-bone transition of the rotator cuff: a preliminary Raman spectroscopic study documenting the gradual mineralization across the insertion in rat tissue samples
    Brigitte Wopenka
    Center for Materials Innovation, Department of Earth and Planetary Sciences, Washington University, St Louis, Missouri 63130, USA
    Appl Spectrosc 62:1285-94. 2008
  10. pmc The effect of muscle loading on flexor tendon-to-bone healing in a canine model
    Stavros Thomopoulos
    Department of Orthopedic Surgery, Washington University, One Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St Louis, Missouri 63110, USA
    J Orthop Res 26:1611-7. 2008

Collaborators

Detail Information

Publications52

  1. ncbi request reprint The development of structural and mechanical anisotropy in fibroblast populated collagen gels
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University School of Medicine, 1 Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St, Louis, MO 63110, USA
    J Biomech Eng 127:742-50. 2005
    ..This model system will provide a basis for investigating structure-function relationships in engineered constructs and for studying mechanisms underlying the development of anisotropy in collagenous tissues...
  2. ncbi request reprint Effect of several growth factors on canine flexor tendon fibroblast proliferation and collagen synthesis in vitro
    Stavros Thomopoulos
    Department of Orthopaedic Research, Washington University, St Louis, MO, USA
    J Hand Surg Am 30:441-7. 2005
    ..Our second hypothesis was that there would be a positive effect from the combination of PDGF-BB and bFGF...
  3. ncbi request reprint Collagen fiber orientation at the tendon to bone insertion and its influence on stress concentrations
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, 1 Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St Louis, MO 63110, USA
    J Biomech 39:1842-51. 2006
    ....
  4. pmc Enhanced flexor tendon healing through controlled delivery of PDGF-BB
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri, USA
    J Orthop Res 27:1209-15. 2009
    ..In conclusion, PDGF-BB treatment consistently improved the functional but not the structural properties of sutured intrasynovial tendons through 42 days following repair...
  5. pmc Controlled-release kinetics and biologic activity of platelet-derived growth factor-BB for use in flexor tendon repair
    Shelly E Sakiyama-Elbert
    Department of Orthopaedic Surgery, Washington University in St Louis, St Louis, MO, USA
    J Hand Surg Am 33:1548-57. 2008
    ....
  6. ncbi request reprint PDGF-BB released in tendon repair using a novel delivery system promotes cell proliferation and collagen remodeling
    Stavros Thomopoulos
    Department of Orthopaedics, Washington University, St Louis, Missouri 63110, USA
    J Orthop Res 25:1358-68. 2007
    ..Sustained delivery of growth factors may be achieved using a novel fibrin-based delivery system. PDGF-BB delivery increased cell proliferation and matrix remodeling and thus may accelerate flexor tendon healing...
  7. pmc Use of a magnesium-based bone adhesive for flexor tendon-to-bone healing
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
    J Hand Surg Am 34:1066-73. 2009
    ..Therefore, we hypothesized that magnesium-based bone adhesive (MBA) will improve the tendon-to-bone biomechanical properties initially and in the early period after repair...
  8. pmc The effects of exogenous basic fibroblast growth factor on intrasynovial flexor tendon healing in a canine model
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St Louis, MO 63110
    J Bone Joint Surg Am 92:2285-93. 2010
    ..The application of growth factors holds promise for improving the tendon-repair response and obviating failure in the initial three weeks...
  9. pmc The tendon-to-bone transition of the rotator cuff: a preliminary Raman spectroscopic study documenting the gradual mineralization across the insertion in rat tissue samples
    Brigitte Wopenka
    Center for Materials Innovation, Department of Earth and Planetary Sciences, Washington University, St Louis, Missouri 63130, USA
    Appl Spectrosc 62:1285-94. 2008
    ..Our finding is also consistent with recent mechanical models and calculations developed to better understand the materials properties of this unusually strong interface...
  10. pmc The effect of muscle loading on flexor tendon-to-bone healing in a canine model
    Stavros Thomopoulos
    Department of Orthopedic Surgery, Washington University, One Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St Louis, Missouri 63110, USA
    J Orthop Res 26:1611-7. 2008
    ..Complete removal of load by proximal transection resulted in tendon-to-bone repairs with less range of motion and lower biomechanical properties compared to repairs in which the muscle-tendon-bone unit was left intact...
  11. ncbi request reprint Early healing of flexor tendon insertion site injuries: Tunnel repair is mechanically and histologically inferior to surface repair in a canine model
    Matthew J Silva
    Department of Orthopaedic Surgery, Washington University School of Medicine, 1 Barnes Jewish Hospital Plaza, Suite 11300 WP, St Louis, Missouri 63110, USA
    J Orthop Res 24:990-1000. 2006
    ..If the results of our animal study extend to healing of the human FDP insertion, they indicate that FDP tendons should be reattached to the distal phalanx by suture to the cortical surface rather than suture in a bone tunnel...
  12. doi request reprint Sustained delivery of transforming growth factor beta three enhances tendon-to-bone healing in a rat model
    Cionne N Manning
    Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St Louis, Missouri 63110, USA
    J Orthop Res 29:1099-105. 2011
    ..We concluded that TGF-β3 delivered at a sustained rate using a HBDS enhanced tendon-to-bone healing in a rat model...
  13. pmc bFGF and PDGF-BB for tendon repair: controlled release and biologic activity by tendon fibroblasts in vitro
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St Louis, MO 63110, USA
    Ann Biomed Eng 38:225-34. 2010
    ..Both growth factors may be effective in enhancing tendon healing in vivo...
  14. doi request reprint The role of transforming growth factor beta isoforms in tendon-to-bone healing
    H Mike Kim
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri 63110, USA
    Connect Tissue Res 52:87-98. 2011
    ....
  15. ncbi request reprint Alendronate prevents bone loss and improves tendon-to-bone repair strength in a canine model
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, 1 Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St Louis, Missouri 63110, USA
    J Orthop Res 25:473-9. 2007
    ..These initial short-term data demonstrate the potential for a clinical treatment that could enhance tendon-to-bone healing...
  16. pmc Musculoskeletal deformities secondary to neurotomy of the superior trunk of the brachial plexus in neonatal mice
    H Mike Kim
    Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St Louis, Missouri 63110, USA
    J Orthop Res 28:1391-8. 2010
    ..The current study presents a useful animal model for future research examining musculoskeletal changes secondary to neonatal nerve injury...
  17. pmc The early inflammatory response after flexor tendon healing: a gene expression and histological analysis
    Cionne N Manning
    Department of Orthopaedic Surgery, Washington University, 425 South Euclid, Campus Box 8233, St Louis, Missouri, 63110
    J Orthop Res 32:645-52. 2014
    ..These findings suggest that future tendon treatment approaches consider modulation of the inflammatory phase of healing...
  18. pmc Tissue-engineering strategies for the tendon/ligament-to-bone insertion
    Lester Smith
    Department of Orthopaedic Surgery, Washington University, St Louis, MO, USA
    Connect Tissue Res 53:95-105. 2012
    ..The focus lies on strategies for producing mechanical structures that can guide and subsequently sustain a graded tissue structure and the associated cell populations...
  19. doi request reprint Complete removal of load is detrimental to rotator cuff healing
    Leesa M Galatz
    Department of Orthopaedic Surgery, Washington University at Barnes Jewish Hospital, St Louis, MO 63110, USA
    J Shoulder Elbow Surg 18:669-75. 2009
    ..This study evaluated the effect of the mechanical environment on the healing rotator cuff by paralyzing the supraspinatus muscle in the operative shoulder of a rat model of rotator cuff injury and repair...
  20. pmc BMP12 induces tenogenic differentiation of adipose-derived stromal cells
    Hua Shen
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri, United States of America
    PLoS ONE 8:e77613. 2013
    ..Collectively, results support the conclusion that BMP12 induces tenogenic differentiation of ASCs via the Smad1/5/8 pathway. ..
  21. pmc Mineral distributions at the developing tendon enthesis
    Andrea G Schwartz
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri, United States of America
    PLoS ONE 7:e48630. 2012
    ..These conserved and time-varying aspects of interface composition may have important implications for the growth and mechanical stability of the tendon-to-bone attachment throughout development...
  22. pmc Effect of bone morphogenetic protein 2 on tendon-to-bone healing in a canine flexor tendon model
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
    J Orthop Res 30:1702-9. 2012
    ..The anticipated anabolic effect of BMP-2 was insufficient in the short time frame of this study to counter the post-repair loss of bone...
  23. pmc Technical and biological modifications for enhanced flexor tendon repair
    H Mike Kim
    Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
    J Hand Surg Am 35:1031-7; quiz 1038. 2010
    ..In this review, we describe recent efforts to enhance the integrity of the immature repair site. We focus on 2 major areas of advancement: surgical technique modifications and manipulation of the biologic and biochemical environment...
  24. ncbi request reprint Collagen fiber alignment does not explain mechanical anisotropy in fibroblast populated collagen gels
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University School of Medicine, 1 Barnes Jewish Hospital Plaza, Suite 11300, Campus Box 8233, St Louis, MO 63110, USA
    J Biomech Eng 129:642-50. 2007
    ....
  25. pmc "Aligned-to-random" nanofiber scaffolds for mimicking the structure of the tendon-to-bone insertion site
    Jingwei Xie
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    Nanoscale 2:923-6. 2010
    ..Tendon fibroblasts cultured on such a scaffold exhibited highly organized and haphazardly oriented morphologies, respectively, on the aligned and random portions...
  26. pmc Functional grading of mineral and collagen in the attachment of tendon to bone
    Guy M Genin
    Department of Mechanical Aerospace and Structural Engineering, Washington University, St Louis, Missouri 63130, USA
    Biophys J 97:976-85. 2009
    ..Our models explain how tendon-to-bone attachment is achieved through a functionally graded material composition, and provide targets for tissue engineered surgical interventions and biomimetic material interfaces...
  27. pmc The effects of chronic unloading and gap formation on tendon-to-bone healing in a rat model of massive rotator cuff tears
    Megan L Killian
    Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St Louis, Missouri, 63110
    J Orthop Res 32:439-47. 2014
    ..Massive tear in a rodent model resulted in gap formation regardless of injury duration which had detrimental effects on repair outcomes...
  28. pmc The effect of core and epitendinous suture modifications on repair of intrasynovial flexor tendons in an in vivo canine model
    Duretti T Fufa
    Washington University School of Medicine, St Louis, MO 63110, USA
    J Hand Surg Am 37:2526-31. 2012
    ..Our null hypothesis was that gap formation and rupture rates would remain consistent across repair techniques...
  29. ncbi request reprint The early effects of sustained platelet-derived growth factor administration on the functional and structural properties of repaired intrasynovial flexor tendons: an in vivo biomechanic study at 3 weeks in canines
    Richard H Gelberman
    Department of Orthopaedic Surgery, Barnes Jewish Hospital at Washington University, St Louis, MO 63110, USA
    J Hand Surg Am 32:373-9. 2007
    ..We hypothesized that PDGF-BB administered in this manner would improve the sutured tendon's functional and structural properties 3 weeks after repair...
  30. doi request reprint Recovery potential after postnatal shoulder paralysis. An animal model of neonatal brachial plexus palsy
    H Mike Kim
    Department of Orthopaedic Surgery, Washington University, One Barnes Jewish Hospital Plaza, 11300 West Pavilion, Campus Box 8233, St Louis, MO 63110, USA
    J Bone Joint Surg Am 91:879-91. 2009
    ..The goal of this study was to characterize the development of bone and joint deformities in paralyzed neonatal shoulders and to assess the improvement of these deformities after muscle function recovery with use of an animal model...
  31. pmc In vitro mineralization by preosteoblasts in poly(DL-lactide-co-glycolide) inverse opal scaffolds reinforced with hydroxyapatite nanoparticles
    Sung Wook Choi
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    Langmuir 26:12126-31. 2010
    ..The Ap-coated PLGA/HAp scaffold with uniform pore structure could be a promising material for bone tissue engineering...
  32. ncbi request reprint Decreased muscle loading delays maturation of the tendon enthesis during postnatal development
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri 63110, USA
    J Orthop Res 25:1154-63. 2007
    ..Physical factors did not play a significant role in enthesis maturation in the first 14 days postnatally, implying that biologic factors may drive early postnatal development...
  33. pmc Enhancing the stiffness of electrospun nanofiber scaffolds with a controlled surface coating and mineralization
    Wenying Liu
    Department of Energy, Environmental and Chemical Engineering, Washington University, St Louis, Missouri, USA
    Langmuir 27:9088-93. 2011
    ..A combination of the nanoscale fibrous structure and bonelike mineral coating could mimic the structure, composition, and function of mineralized tissues...
  34. pmc The effect of tear size and nerve injury on rotator cuff muscle fatty degeneration in a rodent animal model
    H Mike Kim
    Department of Orthopaedic Surgery, Washington University, St Louis, MO, USA
    J Shoulder Elbow Surg 21:847-58. 2012
    ....
  35. pmc The nanometre-scale physiology of bone: steric modelling and scanning transmission electron microscopy of collagen-mineral structure
    BENJAMIN ALEXANDER
    Department of Mechanical Engineering and Materials Science, Washington University, Saint Louis, MO 63130, USA
    J R Soc Interface 9:1774-86. 2012
    ..These results have important implications for the mechanics of partially mineralized and developing tissues...
  36. pmc Intrasynovial flexor tendon repair: a biomechanical study of variations in suture application in human cadavera
    Gregory N Nelson
    Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
    J Orthop Res 30:1652-9. 2012
    ....
  37. pmc The role of mechanobiology in tendon healing
    Megan L Killian
    Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
    J Shoulder Elbow Surg 21:228-37. 2012
    ..This review will summarize existing knowledge of the mechanobiology of tendon development, homeostasis, and healing...
  38. pmc The role of muscle loading on bone (Re)modeling at the developing enthesis
    Alexander M Tatara
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri, United States of America
    PLoS ONE 9:e97375. 2014
    ....
  39. pmc The effect of suture caliber and number of core suture strands on zone II flexor tendon repair: a study in human cadavers
    Daniel A Osei
    Department of Orthopedic Surgery, Washington University School of Medicine, and the Washington University Institute of Clinical and Translational Sciences, St Louis, Mo Electronic address
    J Hand Surg Am 39:262-8. 2014
    ..To compare the tensile properties of a 3-0, 4-strand flexor tendon repair with a 4-0, 4-strand repair and a 4-0, 8-strand repair...
  40. pmc Electrospun nanofibers for regenerative medicine
    Wenying Liu
    Department of Energy, Environmental and Chemical Engineering, Washington University in St Louis, St Louis, MO 63130, USA
    Adv Healthc Mater 1:10-25. 2012
    ..The Progress Report concludes with perspectives on challenges and future directions for design, fabrication, and utilization of scaffolds based on electrospun nanofibers...
  41. ncbi request reprint Development of the supraspinatus tendon-to-bone insertion: localized expression of extracellular matrix and growth factor genes
    Leesa Galatz
    Washington University Orthopaedics, Barnes Jewish Hospital, Campus Box 8233, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
    J Orthop Res 25:1621-8. 2007
    ..Type X collagen was first expressed by the chondrocytes in the mineralized fibrocartilage at 14 days and persisted in the mineralized fibrocartilage at 56 days. A shift from TGF-beta3 to TGF-beta1 expression occurred at 15.5 dpc...
  42. pmc Recent advances in shoulder research
    Megan L Killian
    Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
    Arthritis Res Ther 14:214. 2012
    ..Future treatment modalities involving biologics and tissue engineering hold further promise to improve outcomes for patients suffering from shoulder pathologies...
  43. ncbi request reprint Characteristics of the rat supraspinatus tendon during tendon-to-bone healing after acute injury
    Leesa M Galatz
    Department of Orthopaedic Research, Washington University School of Medicine, Barnes Jewish Hospital, 660 S Euclid Avenue, Campus Box 8233, St Louis, Missouri 63110, USA
    J Orthop Res 24:541-50. 2006
    ..Repair sites remained unorganized histologically and biomechanically inferior in comparison to previously described uninjured insertion sites...
  44. pmc Effects of botulinum toxin-induced paralysis on postnatal development of the supraspinatus muscle
    Rosalina Das
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri, USA
    J Orthop Res 29:281-8. 2011
    ..Understanding the molecular and compositional changes in developing supraspinatus muscles may be useful for identifying and addressing the pathological changes that occur in shoulder injuries such as neonatal brachial plexus palsy...
  45. pmc Fibrocartilage tissue engineering: the role of the stress environment on cell morphology and matrix expression
    Stavros Thomopoulos
    Department of Orthopaedic Surgery, Washington University, St Louis, Missouri 63110, USA
    Tissue Eng Part A 17:1039-53. 2011
    ..These studies support the hypothesis that the 3D stress environment influences cell activity and gene expression in fibrocartilage development...
  46. pmc Nanofiber scaffolds with gradations in mineral content for mimicking the tendon-to-bone insertion site
    Xiaoran Li
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    Nano Lett 9:2763-8. 2009
    ..This new class of nanofiber-based scaffolds can potentially be employed for repairing the tendon-to-bone insertion site via a tissue engineering approach...
  47. pmc The developing shoulder has a limited capacity to recover after a short duration of neonatal paralysis
    Ryan Potter
    Department of Orthopaedic Surgery, Washington University, 660 South Euclid, Campus Box 8233, St Louis, MO 63110, USA
    J Biomech 47:2314-20. 2014
    ....
  48. pmc Modelling the mechanics of partially mineralized collagen fibrils, fibres and tissue
    Yanxin Liu
    Department of Mechanical Engineering and Materials Science, Washington University, St Louis, MO 63130, USA
    J R Soc Interface 11:20130835. 2014
    ..These models and observations have implications for engineered tissue scaffolds at the attachment of tendon to bone, bone development and graded biomimetic attachment of dissimilar hierarchical materials in general. ..
  49. ncbi request reprint Decreased collagen organization and content are associated with reduced strength of demineralized and intact bone in the SAMP6 mouse
    Matthew J Silva
    Department of Orthopaedic Surgery, Barnes Jewish Hospital at Washington University, St Louis, Missouri 63110, USA
    J Bone Miner Res 21:78-88. 2006
    ..The matrix weakness is attributed primarily to poorer organization of collagen fibers and reduced collagen content...
  50. ncbi request reprint The effect of fibrin clot on healing rat supraspinatus tendon defects
    Stavros Thomopoulos
    Orthopaedic Research Laboratories, University of Michigan, Ann Arbor, MI, USA
    J Shoulder Elbow Surg 11:239-47. 2002
    ..There was no effect from addition of the fibrin clot except at 3 weeks, where it led to a decrease in material properties...
  51. ncbi request reprint Variation of biomechanical, structural, and compositional properties along the tendon to bone insertion site
    Stavros Thomopoulos
    McKay Orthopaedic Research Laboratory, University of Pennsylvania, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia 19104 6081, USA
    J Orthop Res 21:413-9. 2003
    ..Thus, the tendon to bony insertion site varies dramatically along its length in terms of its viscoelastic properties, collagen structure, and extracellular matrix composition...
  52. ncbi request reprint Rotator cuff tendinosis in an animal model: role of extrinsic and overuse factors
    Louis J Soslowsky
    McKay Orthopaedic Research Laboratory, University of Pennsylvania, 424 Stemmler Hall, Philadelphia, PA 19104 6081, USA
    Ann Biomed Eng 30:1057-63. 2002
    ..The results of the present study support the role of multiple factors in the etiology of some rotator cuff injuries...