LINDA JOY PIKE

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc Oligomerization of the EGF receptor investigated by live cell fluorescence intensity distribution analysis
    Saveez Saffarian
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02155, USA
    Biophys J 93:1021-31. 2007
  2. pmc Negative co-operativity in the EGF receptor
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    Biochem Soc Trans 40:15-9. 2012
  3. pmc The challenge of lipid rafts
    Linda J Pike
    Washington University School of Medicine Department of Biochemistry and Molecular Biophysics, St Louis, MO 63110, USA
    J Lipid Res 50:S323-8. 2009
  4. ncbi request reprint Cholesterol depletion delocalizes phosphatidylinositol bisphosphate and inhibits hormone-stimulated phosphatidylinositol turnover
    L J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 273:22298-304. 1998
  5. pmc Lipid rafts: heterogeneity on the high seas
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid Avenue, Box 8231, St Louis, MO 63110, USA
    Biochem J 378:281-92. 2004
  6. ncbi request reprint Lipid rafts: bringing order to chaos
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    J Lipid Res 44:655-67. 2003
  7. ncbi request reprint Cholesterol levels modulate EGF receptor-mediated signaling by altering receptor function and trafficking
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:10315-22. 2002
  8. ncbi request reprint Lipid rafts are enriched in arachidonic acid and plasmenylethanolamine and their composition is independent of caveolin-1 expression: a quantitative electrospray ionization/mass spectrometric analysis
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:2075-88. 2002
  9. pmc Cholesterol depletion results in site-specific increases in epidermal growth factor receptor phosphorylation due to membrane level effects. Studies with cholesterol enantiomers
    Emily J Westover
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:51125-33. 2003
  10. pmc Palmitoylation of the EGF receptor impairs signal transduction and abolishes high-affinity ligand binding
    Jennifer L Macdonald-Obermann
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 48:2505-13. 2009

Research Grants

  1. Lipid Rafts and EGF Receptor Function
    LINDA JOY PIKE; Fiscal Year: 2010
  2. EGF Receptor Activation and Interaction with ErbB Family Receptors
    Linda Pike; Fiscal Year: 2009
  3. Lipid Rafts and EGF Receptor Function
    Linda Pike; Fiscal Year: 2009
  4. Lipid Rafts and EGF Receptor Function
    Linda Pike; Fiscal Year: 2007
  5. Lipid Rafts and Cell Function
    Linda Pike; Fiscal Year: 2006
  6. Lipid Rafts and EGF Receptor Function
    Linda Pike; Fiscal Year: 2005
  7. EGF Receptor Activation and Interaction with ErbB Family Receptors
    LINDA JOY PIKE; Fiscal Year: 2010

Collaborators

  • Richard Gross
  • X Han
  • HOWARD BROCKMAN
  • Mark A Watson
  • T Wiedmer
  • Jun Sun
  • Katherine S Yang
  • Jennifer L Macdonald-Obermann
  • Jennifer L Macdonald
  • Robert Y C Yang
  • David Piwnica-Worms
  • Martin Eiblmaier
  • Saveez Saffarian
  • Jennifer Macdonald
  • Emily J Westover
  • Garland R Marshall
  • Ma Xenia G Ilagan
  • Laura A Meyer
  • Paula M Fracasso
  • Carolyn J Anderson
  • Yu Li
  • Elliot L Elson
  • Wanwen Su
  • Zhengzhe Li
  • Rhoderick E Brown
  • Douglas F Covey

Detail Information

Publications22

  1. pmc Oligomerization of the EGF receptor investigated by live cell fluorescence intensity distribution analysis
    Saveez Saffarian
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02155, USA
    Biophys J 93:1021-31. 2007
    ..Acute depletion of cellular cholesterol enhanced EGF receptor clustering whereas cholesterol loading decreased receptor clustering, indicating that receptor aggregation is sensitive to the lipid composition of the membrane...
  2. pmc Negative co-operativity in the EGF receptor
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    Biochem Soc Trans 40:15-9. 2012
    ..Binding of EGF to its receptor is positively linked with dimer assembly, but shows negative co-operativity within the dimer...
  3. pmc The challenge of lipid rafts
    Linda J Pike
    Washington University School of Medicine Department of Biochemistry and Molecular Biophysics, St Louis, MO 63110, USA
    J Lipid Res 50:S323-8. 2009
    ..It also outlines the contribution of single-molecule methods in defining the forces that drive the formation and dynamics of these membrane domains...
  4. ncbi request reprint Cholesterol depletion delocalizes phosphatidylinositol bisphosphate and inhibits hormone-stimulated phosphatidylinositol turnover
    L J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 273:22298-304. 1998
    ..These data are consistent with the hypothesis that localization of signaling proteins and lipids to cholesterol-enriched domains is required for the proper function of hormone-stimulated PtdIns turnover...
  5. pmc Lipid rafts: heterogeneity on the high seas
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid Avenue, Box 8231, St Louis, MO 63110, USA
    Biochem J 378:281-92. 2004
    ..Based on differences in the ways in which proteins interact with rafts, the Induced-Fit Model of Raft Heterogeneity is proposed to explain the establishment and maintenance of heterogeneity within raft populations...
  6. ncbi request reprint Lipid rafts: bringing order to chaos
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    J Lipid Res 44:655-67. 2003
    ..This review provides an overview of the physical characteristics of lipid rafts and summarizes studies that have helped to elucidate the role of lipid rafts in signaling via receptor tyrosine kinases and G protein-coupled receptors...
  7. ncbi request reprint Cholesterol levels modulate EGF receptor-mediated signaling by altering receptor function and trafficking
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:10315-22. 2002
    ..Cholesterol depletion also inhibits EGF internalization and down-regulation of the EGF receptor, and this likely contributes to the enhanced ability of EGF to stimulate downstream signaling pathways such as the activation of MAP kinase...
  8. ncbi request reprint Lipid rafts are enriched in arachidonic acid and plasmenylethanolamine and their composition is independent of caveolin-1 expression: a quantitative electrospray ionization/mass spectrometric analysis
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:2075-88. 2002
    ..These data provide insight into the structure of lipid rafts and identify potential new roles for these domains in signal transduction...
  9. pmc Cholesterol depletion results in site-specific increases in epidermal growth factor receptor phosphorylation due to membrane level effects. Studies with cholesterol enantiomers
    Emily J Westover
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:51125-33. 2003
    ..These data suggest that cholesterol most likely affects EGF receptor function because of its physical effects on membrane properties, not through direct enantioselective interactions with the receptor...
  10. pmc Palmitoylation of the EGF receptor impairs signal transduction and abolishes high-affinity ligand binding
    Jennifer L Macdonald-Obermann
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 48:2505-13. 2009
    ....
  11. pmc Heterogeneity in EGF-binding affinities arises from negative cooperativity in an aggregating system
    Jennifer L Macdonald
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 105:112-7. 2008
    ....
  12. ncbi request reprint Rafts defined: a report on the Keystone Symposium on Lipid Rafts and Cell Function
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:1597-8. 2006
    ..quot; This definition helps to clarify current thinking in a field that has been plagued by the heterogeneous and sometimes ephemeral nature of its subject...
  13. pmc Luciferase fragment complementation imaging of conformational changes in the epidermal growth factor receptor
    Katherine S Yang
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 284:7474-82. 2009
    ..Our data demonstrate the utility of the luciferase system for in vivo imaging changes in EGF receptor dimerization and conformation. They also identify two sequential ligand-induced conformational changes in the EGF receptor...
  14. pmc Epidermal growth factor receptors are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: a shotgun lipidomics study
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26796-804. 2005
    ....
  15. ncbi request reprint Growth factor receptors, lipid rafts and caveolae: an evolving story
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    Biochim Biophys Acta 1746:260-73. 2005
    ....
  16. pmc The intracellular juxtamembrane domain of the epidermal growth factor (EGF) receptor is responsible for the allosteric regulation of EGF binding
    Jennifer L Macdonald-Obermann
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:13570-6. 2009
    ..W., and Carpenter, G. (2007) Proc. Natl. Acad. Sci. U. S. A. 104, 19238-19243). Our experiments expand the role of this domain to include the allosteric control of ligand binding by the extracellular domain...
  17. pmc Asp-960/Glu-961 controls the movement of the C-terminal tail of the epidermal growth factor receptor to regulate asymmetric dimer formation
    Katherine S Yang
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 285:24014-22. 2010
    ..Mutation of these residues destabilizes this hinge, facilitating the formation of the activating asymmetric dimer and leading to enhanced receptor signaling...
  18. pmc The membrane proximal disulfides of the EGF receptor extracellular domain are required for high affinity binding and signal transduction but do not play a role in the localization of the receptor to lipid rafts
    Jennifer Macdonald
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, MO 63110, USA
    Biochim Biophys Acta 1763:870-8. 2006
    ....
  19. ncbi request reprint A simplified method for the preparation of detergent-free lipid rafts
    Jennifer L Macdonald
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Lipid Res 46:1061-7. 2005
    ....
  20. doi request reprint Correlating EGFR expression with receptor-binding properties and internalization of 64Cu-DOTA-cetuximab in 5 cervical cancer cell lines
    Martin Eiblmaier
    Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Nucl Med 49:1472-9. 2008
    ....
  21. pmc Targeting the dimerization of epidermal growth factor receptors with small-molecule inhibitors
    Robert Y C Yang
    Department of Biochemistry and Molecular Biophysics, Washington University in St Louis School of Medicine, St Louis, MO 63110, USA
    Chem Biol Drug Des 76:1-9. 2010
    ..This first-generation lead compound represents the first drug-like, small-molecule inhibitor of EGF receptor activation that is not directed against the intracellular kinase domain...
  22. ncbi request reprint Plasma membrane phospholipid scramblase 1 is enriched in lipid rafts and interacts with the epidermal growth factor receptor
    Jun Sun
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 41:6338-45. 2002
    ....

Research Grants12

  1. Lipid Rafts and EGF Receptor Function
    LINDA JOY PIKE; Fiscal Year: 2010
    ....
  2. EGF Receptor Activation and Interaction with ErbB Family Receptors
    Linda Pike; Fiscal Year: 2009
    ..abstract_text> ..
  3. Lipid Rafts and EGF Receptor Function
    Linda Pike; Fiscal Year: 2009
    ....
  4. Lipid Rafts and EGF Receptor Function
    Linda Pike; Fiscal Year: 2007
    ....
  5. Lipid Rafts and Cell Function
    Linda Pike; Fiscal Year: 2006
    ..and, iv) the role of rafts in endocytosis and protein processing. ..
  6. Lipid Rafts and EGF Receptor Function
    Linda Pike; Fiscal Year: 2005
    ..They will also delineate the effect of raft localization on EGF receptor function. These findings will provide insight into the contribution of lipid rafts to the regulation of EGF receptor-mediated signaling. ..
  7. EGF Receptor Activation and Interaction with ErbB Family Receptors
    LINDA JOY PIKE; Fiscal Year: 2010
    ..abstract_text> ..