Linda J Pike

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi request reprint Rafts defined: a report on the Keystone Symposium on Lipid Rafts and Cell Function
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:1597-8. 2006
  2. ncbi request reprint Growth factor receptors, lipid rafts and caveolae: an evolving story
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    Biochim Biophys Acta 1746:260-73. 2005
  3. pmc Epidermal growth factor receptors are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: a shotgun lipidomics study
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26796-804. 2005
  4. pmc Heterogeneity in EGF-binding affinities arises from negative cooperativity in an aggregating system
    Jennifer L Macdonald
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 105:112-7. 2008
  5. pmc The tethering arm of the EGF receptor is required for negative cooperativity and signal transduction
    Sangeeta Adak
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 286:1545-55. 2011
  6. pmc Palmitoylation of the EGF receptor impairs signal transduction and abolishes high-affinity ligand binding
    Jennifer L Macdonald-Obermann
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 48:2505-13. 2009
  7. pmc The membrane-proximal intracellular domain of the epidermal growth factor receptor underlies negative cooperativity in ligand binding
    Sangeeta Adak
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 286:45146-55. 2011
  8. pmc Quantitation of the effect of ErbB2 on epidermal growth factor receptor binding and dimerization
    Yu Li
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 287:31116-25. 2012
  9. pmc Mechanics of EGF receptor/ErbB2 kinase activation revealed by luciferase fragment complementation imaging
    Jennifer L Macdonald-Obermann
    Department of Biochemistry, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 109:137-42. 2012
  10. pmc Asp-960/Glu-961 controls the movement of the C-terminal tail of the epidermal growth factor receptor to regulate asymmetric dimer formation
    Katherine S Yang
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 285:24014-22. 2010

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Rafts defined: a report on the Keystone Symposium on Lipid Rafts and Cell Function
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:1597-8. 2006
    ..quot; This definition helps to clarify current thinking in a field that has been plagued by the heterogeneous and sometimes ephemeral nature of its subject...
  2. ncbi request reprint Growth factor receptors, lipid rafts and caveolae: an evolving story
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    Biochim Biophys Acta 1746:260-73. 2005
    ....
  3. pmc Epidermal growth factor receptors are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: a shotgun lipidomics study
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26796-804. 2005
    ....
  4. pmc Heterogeneity in EGF-binding affinities arises from negative cooperativity in an aggregating system
    Jennifer L Macdonald
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 105:112-7. 2008
    ....
  5. pmc The tethering arm of the EGF receptor is required for negative cooperativity and signal transduction
    Sangeeta Adak
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 286:1545-55. 2011
    ..Because cooperativity implies subunit-subunit interactions, these results also suggest that the tethering arm contributes to intersubunit interactions within the EGF receptor dimer...
  6. pmc Palmitoylation of the EGF receptor impairs signal transduction and abolishes high-affinity ligand binding
    Jennifer L Macdonald-Obermann
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 48:2505-13. 2009
    ....
  7. pmc The membrane-proximal intracellular domain of the epidermal growth factor receptor underlies negative cooperativity in ligand binding
    Sangeeta Adak
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 286:45146-55. 2011
    ..Thus, some of the energy of binding to the second site is used to reorient the first asymmetric dimer, leading to a lower binding affinity and the observed negative cooperativity...
  8. pmc Quantitation of the effect of ErbB2 on epidermal growth factor receptor binding and dimerization
    Yu Li
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 287:31116-25. 2012
    ....
  9. pmc Mechanics of EGF receptor/ErbB2 kinase activation revealed by luciferase fragment complementation imaging
    Jennifer L Macdonald-Obermann
    Department of Biochemistry, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 109:137-42. 2012
    ..Thus, activation of the kinases in the EGFR/ErbB2 asymmetric dimer occurs in a specific sequence and depends on the kinase activity of the EGF receptor...
  10. pmc Asp-960/Glu-961 controls the movement of the C-terminal tail of the epidermal growth factor receptor to regulate asymmetric dimer formation
    Katherine S Yang
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 285:24014-22. 2010
    ..Mutation of these residues destabilizes this hinge, facilitating the formation of the activating asymmetric dimer and leading to enhanced receptor signaling...
  11. pmc The intracellular juxtamembrane domain of the epidermal growth factor (EGF) receptor is responsible for the allosteric regulation of EGF binding
    Jennifer L Macdonald-Obermann
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:13570-6. 2009
    ..W., and Carpenter, G. (2007) Proc. Natl. Acad. Sci. U. S. A. 104, 19238-19243). Our experiments expand the role of this domain to include the allosteric control of ligand binding by the extracellular domain...
  12. pmc Cholesterol depletion results in site-specific increases in epidermal growth factor receptor phosphorylation due to membrane level effects. Studies with cholesterol enantiomers
    Emily J Westover
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:51125-33. 2003
    ..These data suggest that cholesterol most likely affects EGF receptor function because of its physical effects on membrane properties, not through direct enantioselective interactions with the receptor...
  13. pmc Luciferase fragment complementation imaging of conformational changes in the epidermal growth factor receptor
    Katherine S Yang
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Biol Chem 284:7474-82. 2009
    ..Our data demonstrate the utility of the luciferase system for in vivo imaging changes in EGF receptor dimerization and conformation. They also identify two sequential ligand-induced conformational changes in the EGF receptor...
  14. pmc Negative co-operativity in the EGF receptor
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    Biochem Soc Trans 40:15-9. 2012
    ..Binding of EGF to its receptor is positively linked with dimer assembly, but shows negative co-operativity within the dimer...
  15. pmc The membrane proximal disulfides of the EGF receptor extracellular domain are required for high affinity binding and signal transduction but do not play a role in the localization of the receptor to lipid rafts
    Jennifer Macdonald
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, MO 63110, USA
    Biochim Biophys Acta 1763:870-8. 2006
    ....
  16. ncbi request reprint Lipid rafts are enriched in arachidonic acid and plasmenylethanolamine and their composition is independent of caveolin-1 expression: a quantitative electrospray ionization/mass spectrometric analysis
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:2075-88. 2002
    ..These data provide insight into the structure of lipid rafts and identify potential new roles for these domains in signal transduction...
  17. pmc Targeting the dimerization of epidermal growth factor receptors with small-molecule inhibitors
    Robert Y C Yang
    Department of Biochemistry and Molecular Biophysics, Washington University in St Louis School of Medicine, St Louis, MO 63110, USA
    Chem Biol Drug Des 76:1-9. 2010
    ..This first-generation lead compound represents the first drug-like, small-molecule inhibitor of EGF receptor activation that is not directed against the intracellular kinase domain...
  18. ncbi request reprint Cholesterol levels modulate EGF receptor-mediated signaling by altering receptor function and trafficking
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:10315-22. 2002
    ..Cholesterol depletion also inhibits EGF internalization and down-regulation of the EGF receptor, and this likely contributes to the enhanced ability of EGF to stimulate downstream signaling pathways such as the activation of MAP kinase...
  19. ncbi request reprint Lipid rafts: bringing order to chaos
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid, Box 8231, St Louis, MO 63110, USA
    J Lipid Res 44:655-67. 2003
    ..This review provides an overview of the physical characteristics of lipid rafts and summarizes studies that have helped to elucidate the role of lipid rafts in signaling via receptor tyrosine kinases and G protein-coupled receptors...
  20. doi request reprint Correlating EGFR expression with receptor-binding properties and internalization of 64Cu-DOTA-cetuximab in 5 cervical cancer cell lines
    Martin Eiblmaier
    Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Nucl Med 49:1472-9. 2008
    ....
  21. ncbi request reprint A simplified method for the preparation of detergent-free lipid rafts
    Jennifer L Macdonald
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Lipid Res 46:1061-7. 2005
    ....
  22. pmc The challenge of lipid rafts
    Linda J Pike
    Washington University School of Medicine Department of Biochemistry and Molecular Biophysics, St Louis, MO 63110, USA
    J Lipid Res 50:S323-8. 2009
    ..It also outlines the contribution of single-molecule methods in defining the forces that drive the formation and dynamics of these membrane domains...
  23. pmc Lipid rafts: heterogeneity on the high seas
    Linda J Pike
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, 660 So Euclid Avenue, Box 8231, St Louis, MO 63110, USA
    Biochem J 378:281-92. 2004
    ..Based on differences in the ways in which proteins interact with rafts, the Induced-Fit Model of Raft Heterogeneity is proposed to explain the establishment and maintenance of heterogeneity within raft populations...
  24. ncbi request reprint Plasma membrane phospholipid scramblase 1 is enriched in lipid rafts and interacts with the epidermal growth factor receptor
    Jun Sun
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 41:6338-45. 2002
    ....
  25. pmc Oligomerization of the EGF receptor investigated by live cell fluorescence intensity distribution analysis
    Saveez Saffarian
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02155, USA
    Biophys J 93:1021-31. 2007
    ..Acute depletion of cellular cholesterol enhanced EGF receptor clustering whereas cholesterol loading decreased receptor clustering, indicating that receptor aggregation is sensitive to the lipid composition of the membrane...