Research Topics
Species | M L NonetSummaryAffiliation: Washington University School of Medicine Country: USA Publications
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Publications
UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesiclesM L Nonet
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Mol Biol Cell 10:2343-60. 1999..We propose that the UNC-11 protein mediates two functions during synaptic vesicle biogenesis: it recruits synaptobrevin to synaptic vesicle membranes and it regulates the size of the budded vesicle during clathrin coat assembly...
Visualization of synaptic specializations in live C. elegans with synaptic vesicle protein-GFP fusionsM L Nonet
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, MO 63110, USA
J Neurosci Methods 89:33-40. 1999..The cell biology underlying the sorting of synaptic vesicle proteins, trafficking of vesicles to terminals, and the development of presynaptic specializations is now more amenable to forward genetic analysis using these synaptic markers...- Rabphilin potentiates soluble N-ethylmaleimide sensitive factor attachment protein receptor function independently of rab3J Staunton
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Neurosci 21:9255-64. 2001..These synergistic interactions were independent of rab3 function and were not observed in rab3-SNARE double mutants. Our data reveal rab3-independent functions for rabphilin in the potentiation of SNARE function... - A post-docking role for active zone protein RimS P Koushika
Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S Euclid Avenue, Saint Louis, Missouri 63110, USA
Nat Neurosci 4:997-1005. 2001..These data suggest Rim acts after vesicle docking likely via regulating priming... - A conserved mechanism of synaptogyrin localizationH Zhao
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Mol Biol Cell 12:2275-89. 2001..Similarly, the C-terminal domain of rat synaptogyrin is necessary for localization in hippocampal neurons. Our study suggests that the mechanisms for synaptogyrin localization are likely to be conserved from C. elegans to vertebrates... - The Caenorhabditis elegans unc-64 locus encodes a syntaxin that interacts genetically with synaptobrevinO Saifee
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Mol Biol Cell 9:1235-52. 1998.... - Dissection of K+ currents in Caenorhabditis elegans muscle cells by genetics and RNA interferenceC M Santi
Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis MO 63110, USA
Proc Natl Acad Sci U S A 100:14391-6. 2003..Hence, the picture that emerges is of a complex membrane with a small number of household conductances functioning under normal circumstances, but with additional conductances that are activated during unusual circumstances... - SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junctionZ w Wang
Department of Anatomy and Neurobiology, Washington University School of Medicine, Campus Box 8108, 660 S Euclid Avenue, St Louis, MO 63110, USA
Neuron 32:867-81. 2001.... - rpm-1, a conserved neuronal gene that regulates targeting and synaptogenesis in C. elegansA M Schaefer
Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
Neuron 26:345-56. 2000..These results indicate that RPM-1 functions cell autonomously during synaptogenesis to regulate neuronal morphology... - Synaptic transmission deficits in Caenorhabditis elegans synaptobrevin mutantsM L Nonet
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Neurosci 18:70-80. 1998..However, our analysis also suggests that transmitter release is not completely eliminated by removal of either one or both v-SNAREs... - Sorting and transport in C. elegans: aA model system with a sequenced genomeS P Koushika
Department of Anatomy and Neurobiology, Box 8108, 660 South Euclid Avenue, Washington University School of Medicine, St Louis, MO 63110, USA
Curr Opin Cell Biol 12:517-23. 2000..New in vivo assays that monitor endocytosis and neuronal transport have been used to delineate roles for several genes in these processes... - Cellular and molecular insights into presynaptic assemblyA M Schaefer
Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8108, St Louis, MO 63110, USA
Curr Opin Neurobiol 11:127-34. 2001..Recent studies that visualize tagged synaptic components in cultured cells and in vivo have identified molecular participants and reveal common features in cellular processes of presynaptic assembly... - SYD-2 Liprin-alpha organizes presynaptic active zone formation through ELKSYa Dai
Department of Molecular, Cell and Developmental Biology, Sinsheimer Laboratories, University of California, 1156 High Street, Santa Cruz, California 95064, USA
Nat Neurosci 9:1479-87. 2006..The mutant SYD-2 shows increased association with ELKS. These results establish a functional dependency for assembly of the presynaptic active zone in which SYD-2 plays a key role... - Presynaptic ryanodine receptors are required for normal quantal size at the Caenorhabditis elegans neuromuscular junctionQiang Liu
Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 06030 3401, USA
J Neurosci 25:6745-54. 2005..These observations suggest that RYRs are essential to normal quantal size and are potential regulators of quantal size... - Resistance to volatile anesthetics by mutations enhancing excitatory neurotransmitter release in Caenorhabditis elegansAmmar H Hawasli
Department of Anesthesiology, Division of Biology and Biomedical Sciences, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
Genetics 168:831-43. 2004..These results strengthen the link between transmitter release and VA action... - Mutations in Caenorhabditis elegans cytoplasmic dynein components reveal specificity of neuronal retrograde cargoSandhya P Koushika
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
J Neurosci 24:3907-16. 2004..These mutants provide a model to assess whether defects in transport of specific cargo mediate neuronal dysfunction... - Defects in synaptic vesicle docking in unc-18 mutantsRobby M Weimer
Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, Utah 84112 0840, USA
Nat Neurosci 6:1023-30. 2003..Rather, we found a reduction of docked vesicles at the active zone in unc-18 mutants, suggesting that UNC-18 functions, directly or indirectly, as a facilitator of vesicle docking... - Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic densityElena O Gracheva
Department of Biological Sciences, University of Illinois at Chicago, 840 West Taylor St, Chicago, IL 60607, United States
Neurosci Lett 444:137-42. 2008..Together these results suggest that vesicles targeted to the presynaptic density by RAB-3/UNC-10 interactions are ideally positioned for efficient calcium-dependent release... - AEXpulsing a retrograde signalMichael L Nonet
Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA
Neuron 33:155-6. 2002..Doi and Iwasaki (this issue of Neuron) implicate AEX-1, a protein distantly related to the UNC-13/Munc13 family, as an attractive candidate regulator of the retrograde release machinery in muscle... - Tomosyn inhibits synaptic vesicle priming in Caenorhabditis elegansElena O Gracheva
Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, USA
PLoS Biol 4:e261. 2006..Furthermore, the synaptic defects of unc-13 mutants are partially suppressed in tom-1 unc-13 double mutants. These data indicate that in the intact nervous system, TOM-1 negatively regulates synaptic vesicle priming... - Slit1a inhibits retinal ganglion cell arborization and synaptogenesis via Robo2-dependent and -independent pathwaysDouglas S Campbell
Department of Neurobiology and Anatomy, University of Utah Medical Center, Salt Lake City, UT 84132, USA
Neuron 55:231-45. 2007..Unlike previous PNS studies showing that Slits promote branching, our results show that Slits inhibit arborization and synaptogenesis in the CNS... - Analysis of synaptic transmission in Caenorhabditis elegans using an aldicarb-sensitivity assayTimothy R Mahoney
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
Nat Protoc 1:1772-7. 2006..This protocol can also be modified to follow the paralytic effects with other pharmacological reagents. The assay can be performed in about 3-6 hours depending on the severity of synaptic transmission defects... - Regulators of kinesin involved in polarized trafficking and axon outgrowthShuo Luo
Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S, Euclid Avenue, Saint Louis, MO 63110, USA
J Biol 5:8. 2006..A small Caenorhabditis elegans coiled-coil protein, UNC-69, has now been shown to interact with UNC-76 and to be involved in axonal (but not dendritic) transport and outgrowth, as well as synapse formation... - Regulation of synaptic transmission by RAB-3 and RAB-27 in Caenorhabditis elegansTimothy R Mahoney
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110, USA
Mol Biol Cell 17:2617-25. 2006.... - SNARE complex zero layer residues are not critical for N-ethylmaleimide-sensitive factor-mediated disassemblyJoshua M Lauer
Department of Cell Biology and Physiology, Washington University School of Medicine, 660 S. Euclid, St. Louis, MO 63110, USA
J Biol Chem 281:14823-32. 2006..These findings show that residues of the zero layer do not play an essential role in NSF-mediated disassembly... - Redundant localization mechanisms of RIM and ELKS in Caenorhabditis elegansScott L Deken
Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
J Neurosci 25:5975-83. 2005..Furthermore, they demonstrate that multiple different protein-protein interactions redundantly anchor both ELKS and RIM to active zones and implicate novel proteins in the formation of the active zone... - Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAiFemke Simmer
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
Curr Biol 12:1317-9. 2002..The rrf-3 strain can be useful to study genes that, in wild-type, do not show a phenotype after RNAi, and it is probably the strain of choice for genome-wide RNAi screens... - Intestinal signaling to GABAergic neurons regulates a rhythmic behavior in Caenorhabditis elegansTimothy R Mahoney
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, MO 63110, USA
Proc Natl Acad Sci U S A 105:16350-5. 2008..We propose a model whereby the intestinal genes aex-4 and aex-5 control the DMP by regulating the secretion of a signal, which activates the neuronal receptor aex-2...