MICHAEL M MUECKLER

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi Analysis of transmembrane segment 10 of the Glut1 glucose transporter by cysteine-scanning mutagenesis and substituted cysteine accessibility
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 277:3498-503. 2002
  2. ncbi Transmembrane segment 5 of the Glut1 glucose transporter is an amphipathic helix that forms part of the sugar permeation pathway
    M Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:10923-6. 1999
  3. ncbi Transmembrane segment 6 of the Glut1 glucose transporter is an outer helix and contains amino acid side chains essential for transport activity
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri, 63110, USA
    J Biol Chem 283:11550-5. 2008
  4. ncbi Transmembrane segment 12 of the Glut1 glucose transporter is an outer helix and is not directly involved in the transport mechanism
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:36993-8. 2006
  5. ncbi Cysteine-scanning mutagenesis and substituted cysteine accessibility analysis of transmembrane segment 4 of the Glut1 glucose transporter
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:39562-8. 2005
  6. ncbi Transmembrane segment 3 of the Glut1 glucose transporter is an outer helix
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:46876-81. 2004
  7. ncbi Analysis of transmembrane segment 8 of the GLUT1 glucose transporter by cysteine-scanning mutagenesis and substituted cysteine accessibility
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:10494-9. 2004
  8. ncbi Relative proximity and orientation of helices 4 and 8 of the GLUT1 glucose transporter
    Arturo Alisio
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:26540-5. 2004
  9. ncbi Indinavir inhibits the glucose transporter isoform Glut4 at physiologic concentrations
    Haruhiko Murata
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    AIDS 16:859-63. 2002
  10. ncbi Indinavir induces acute and reversible peripheral insulin resistance in rats
    Paul W Hruz
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Diabetes 51:937-42. 2002

Research Grants

  1. HIV Protease Inhibitors & Glucose Transport
    Mike Mueckler; Fiscal Year: 2007

Collaborators

  • Paul Hruz
  • William Roach
  • Richard C Hresko
  • Haruhiko Murata
  • Xiao Mei Song
  • Arturo Alisio
  • Abdullah A Osman
  • M Alan Permutt
  • Mitsuyoshi Saito
  • Carol Makepeace
  • Paul Schlesinger

Detail Information

Publications16

  1. ncbi Analysis of transmembrane segment 10 of the Glut1 glucose transporter by cysteine-scanning mutagenesis and substituted cysteine accessibility
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 277:3498-503. 2002
    ..Two-dimensional models for the conformation of the 12 transmembrane helices and the exofacial glucose-binding site of Glut1 are proposed that are consistent with existing experimental data...
  2. ncbi Transmembrane segment 5 of the Glut1 glucose transporter is an amphipathic helix that forms part of the sugar permeation pathway
    M Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 274:10923-6. 1999
    ....
  3. ncbi Transmembrane segment 6 of the Glut1 glucose transporter is an outer helix and contains amino acid side chains essential for transport activity
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri, 63110, USA
    J Biol Chem 283:11550-5. 2008
    ..These data suggest that helix 6 contains amino acid side chains that are critical for transport activity and that structurally analogous outer helices may play distinct roles in the function of membrane transporters...
  4. ncbi Transmembrane segment 12 of the Glut1 glucose transporter is an outer helix and is not directly involved in the transport mechanism
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:36993-8. 2006
    ..Additionally, the pCMBS data indicate that the predicted exoplasmic end of helix 12 is completely exposed to the external solvent when the transporter is in its exofacial configuration...
  5. ncbi Cysteine-scanning mutagenesis and substituted cysteine accessibility analysis of transmembrane segment 4 of the Glut1 glucose transporter
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:39562-8. 2005
    ..Based on these data, we conclude that the exoplasmic end of helix 4 lies outside the inner helical bundle in the exofacial configuration of Glut1...
  6. ncbi Transmembrane segment 3 of the Glut1 glucose transporter is an outer helix
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:46876-81. 2004
    ..An updated two-dimensional model for the orientation of the 12 transmembrane helices and the conformation of the exofacial glucose-binding site of Glut1 is presented that is consistent with existing experimental data...
  7. ncbi Analysis of transmembrane segment 8 of the GLUT1 glucose transporter by cysteine-scanning mutagenesis and substituted cysteine accessibility
    Mike Mueckler
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:10494-9. 2004
    ....
  8. ncbi Relative proximity and orientation of helices 4 and 8 of the GLUT1 glucose transporter
    Arturo Alisio
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:26540-5. 2004
    ..An updated model for the clustering of the transmembrane helices of GLUT1 is presented based on these data...
  9. ncbi Indinavir inhibits the glucose transporter isoform Glut4 at physiologic concentrations
    Haruhiko Murata
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    AIDS 16:859-63. 2002
    ..To determine the relative sensitivities of glucose transporter isoforms to the protease inhibitor indinavir and to determine the kinetic mechanism of indinavir-mediated Glut4 isoform inhibition...
  10. ncbi Indinavir induces acute and reversible peripheral insulin resistance in rats
    Paul W Hruz
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Diabetes 51:937-42. 2002
    ..These data demonstrate that indinavir causes acute and reversible changes in whole-body glucose homeostasis in rats and support the contribution of GLUT4 inhibition to the development of insulin resistance in patients treated with PIs...
  11. ncbi Model of the exofacial substrate-binding site and helical folding of the human Glut1 glucose transporter based on scanning mutagenesis
    Mike Mueckler
    Department of Cell Biology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Biochemistry 48:5934-42. 2009
    ..An updated model is presented for the outward-facing substrate-binding site and relative orientation of the 12 transmembrane helices of Glut1...
  12. ncbi Wolframin expression induces novel ion channel activity in endoplasmic reticulum membranes and increases intracellular calcium
    Abdullah A Osman
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:52755-62. 2003
    ..Disruption of this function may place cells at risk to suffer inappropriate death decisions, thus accounting for the progressive beta-cell loss and neuronal degeneration associated with the disease...
  13. ncbi Phosphoinositide-dependent kinase-2 is a distinct protein kinase enriched in a novel cytoskeletal fraction associated with adipocyte plasma membranes
    Richard C Hresko
    Department of Cell Biology and Physiology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA
    J Biol Chem 278:21615-22. 2003
    ..Our data indicate that this PDK2 activity is the result of a kinase distinct from PDK1 and is not due to autophosphorylation or transphosphorylation of Akt...
  14. ncbi Reconstitution of phosphoinositide 3-kinase-dependent insulin signaling in a cell-free system
    Haruhiko Murata
    Department of Cell Biology and Physiology, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO 63110, USA
    J Biol Chem 278:21607-14. 2003
    ....
  15. ncbi Identification of amino acid residues within the C terminus of the Glut4 glucose transporter that are essential for insulin-stimulated redistribution to the plasma membrane
    Xiao Mei Song
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:12571-85. 2008
    ..We conclude that residues within the IRM are critical for the targeting of Glut4, but not of IRAP, to insulin-responsive intracellular membrane compartments in 3T3-L1 adipocytes...
  16. ncbi mTOR.RICTOR is the Ser473 kinase for Akt/protein kinase B in 3T3-L1 adipocytes
    Richard C Hresko
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:40406-16. 2005
    ..Based on our cell-free kinase and small interference RNA results, we conclude that mTOR complexed to RICTOR is the Ser-473 kinase in 3T3-L1 adipocytes...

Research Grants2

  1. HIV Protease Inhibitors & Glucose Transport
    Mike Mueckler; Fiscal Year: 2007
    ..2) To test the hypothesis that PI-mediated inhibition of glucose transport directly contributes to lipodystrophy by suppressing adipogenesis and/or by enhancing adipocyte apoptosis. ..