R H Mealey

Summary

Affiliation: Washington State University
Country: USA

Publications

  1. ncbi request reprint Cytotoxic T lymphocytes and neutralizing antibody in the control of equine infectious anemia virus
    Travis C McGuire
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164 7040, USA
    Viral Immunol 15:521-31. 2002
  2. pmc Comparative genomic analysis and phylogenetic position of Theileria equi
    Lowell S Kappmeyer
    Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, WA 99164 7030, USA
    BMC Genomics 13:603. 2012
  3. pmc Cloning and large-scale expansion of epitope-specific equine cytotoxic T lymphocytes using an anti-equine CD3 monoclonal antibody and human recombinant IL-2
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Vet Immunol Immunopathol 118:121-8. 2007
  4. pmc Failure of low-dose recombinant human IL-2 to support the survival of virus-specific CTL clones infused into severe combined immunodeficient foals: lack of correlation between in vitro activity and in vivo efficacy
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, United States
    Vet Immunol Immunopathol 121:8-22. 2008
  5. pmc Early detection of dominant Env-specific and subdominant Gag-specific CD8+ lymphocytes in equine infectious anemia virus-infected horses using major histocompatibility complex class I/peptide tetrameric complexes
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Virology 339:110-26. 2005
  6. pmc Adaptive immunity is the primary force driving selection of equine infectious anemia virus envelope SU variants during acute infection
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    J Virol 78:9295-305. 2004
  7. pmc Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Virology 313:537-52. 2003
  8. pmc Viral load and clinical disease enhancement associated with a lentivirus cytotoxic T lymphocyte vaccine regimen
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164 7040, United States
    Vaccine 27:2453-68. 2009
  9. pmc Protective effects of passively transferred merozoite-specific antibodies against Theileria equi in horses with severe combined immunodeficiency
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    Clin Vaccine Immunol 19:100-4. 2012
  10. pmc Immune reconstitution prevents continuous equine infectious anemia virus replication in an Arabian foal with severe combined immunodeficiency: lessons for control of lentiviruses
    R H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Clin Immunol 101:237-47. 2001

Research Grants

  1. EIAV Control by High Avidity Rev-Specific CTL Clones
    ROBERT MEALEY; Fiscal Year: 2005
  2. EIAV Control by DNA Vaccine-Induced CTL
    ROBERT MEALEY; Fiscal Year: 2006
  3. Lentivirus Control by CTL and Neutralizing Antibody
    ROBERT MEALEY; Fiscal Year: 2007

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Cytotoxic T lymphocytes and neutralizing antibody in the control of equine infectious anemia virus
    Travis C McGuire
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164 7040, USA
    Viral Immunol 15:521-31. 2002
  2. pmc Comparative genomic analysis and phylogenetic position of Theileria equi
    Lowell S Kappmeyer
    Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, WA 99164 7030, USA
    BMC Genomics 13:603. 2012
    ..equi relative to sequenced apicomplexan parasites...
  3. pmc Cloning and large-scale expansion of epitope-specific equine cytotoxic T lymphocytes using an anti-equine CD3 monoclonal antibody and human recombinant IL-2
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Vet Immunol Immunopathol 118:121-8. 2007
    ..Our results provide a reliable means to generate large numbers of epitope-specific equine CTL clones that are suitable for use in downstream applications, including functional assays and adoptive transfer studies...
  4. pmc Failure of low-dose recombinant human IL-2 to support the survival of virus-specific CTL clones infused into severe combined immunodeficient foals: lack of correlation between in vitro activity and in vivo efficacy
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, United States
    Vet Immunol Immunopathol 121:8-22. 2008
    ..Successful adoptive immunotherapy with CTL clones in immunodeficient horses will likely require higher doses of rhuIL-2, co-infusion of CD4+ T lymphocytes, or administration of equine IL-2...
  5. pmc Early detection of dominant Env-specific and subdominant Gag-specific CD8+ lymphocytes in equine infectious anemia virus-infected horses using major histocompatibility complex class I/peptide tetrameric complexes
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Virology 339:110-26. 2005
    ....
  6. pmc Adaptive immunity is the primary force driving selection of equine infectious anemia virus envelope SU variants during acute infection
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    J Virol 78:9295-305. 2004
    ..Not only do these data indicate that adaptive immunity primarily drives the selection of EIAV SU variants, but also they demonstrate that other selective forces exist during acute infection...
  7. pmc Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Virology 313:537-52. 2003
    ..These results suggested that the epitope specificity of high- and moderate-avidity CTLm was an important determinant for disease outcome in the EIAV-infected horses examined...
  8. pmc Viral load and clinical disease enhancement associated with a lentivirus cytotoxic T lymphocyte vaccine regimen
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, 99164 7040, United States
    Vaccine 27:2453-68. 2009
    ..Although the mechanisms for enhancement associated with this vaccine regimen remain to be elucidated, these results have important implications for development of lentivirus T cell vaccines...
  9. pmc Protective effects of passively transferred merozoite-specific antibodies against Theileria equi in horses with severe combined immunodeficiency
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
    Clin Vaccine Immunol 19:100-4. 2012
    ..equi merozoite stabilate, delayed time to peak parasitemia occurred. Protective effects were associated with a predominance of passively transferred merozoite-specific IgG3...
  10. pmc Immune reconstitution prevents continuous equine infectious anemia virus replication in an Arabian foal with severe combined immunodeficiency: lessons for control of lentiviruses
    R H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Clin Immunol 101:237-47. 2001
    ..These results provide further direct evidence that a specific immune response is required for termination of plasma viremia in acute lentiviral infections...
  11. pmc A single amino acid difference within the alpha-2 domain of two naturally occurring equine MHC class I molecules alters the recognition of Gag and Rev epitopes by equine infectious anemia virus-specific CTL
    Robert H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
    J Immunol 177:7377-90. 2006
    ....
  12. pmc CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins
    Chungwon Chung
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99165 7040, USA
    Virology 327:144-54. 2004
    ..EC1 and EC3 were the most conserved EC and these more conserved broadly recognized EC may be most useful for CTL induction, helping overcome MHC class I polymorphism and antigenic variation...
  13. pmc Experimental Rhodococcus equi and equine infectious anemia virus DNA vaccination in adult and neonatal horses: effect of IL-12, dose, and route
    R H Mealey
    Department of Veterinary Microbiology and Pathology, Washington State University, College of Veterinary Medicine, Pullman, WA 99164 7040, USA
    Vaccine 25:7582-97. 2007
    ..Additional work is needed to optimize DNA vaccine-induced Th1 responses in horses, especially in neonates...
  14. pmc Development and characterization of an equine infectious anemia virus Env-pseudotyped reporter virus
    R L Tallmadge
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99163, USA
    Clin Vaccine Immunol 15:1138-40. 2008
    ..An EIAV Env pseudovirus will improve antigenic characterization of viral variants and evaluation of lentivirus vaccines...
  15. pmc Evaluation of high functional avidity CTL to Gag epitope clusters in EIAV carrier horses
    Chungwon Chung
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99165 7040, USA
    Virology 342:228-39. 2005
    ..Thus, not all carrier horses had high functional avidity CTL to Gag EC, but those that did had longer periods without disease episodes...
  16. pmc Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge
    Darrilyn G Fraser
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164 7040, USA
    Clin Diagn Lab Immunol 12:983-93. 2005
    ..Thus, lipopeptide-induced proliferative responses and enhanced Th and CTL responses early after virus challenge were unable to control challenge virus load and clinical disease...
  17. ncbi request reprint Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses
    T C McGuire
    Department of Veterinary Microbiology and Pathology and Department of Animal Sciences, Washington State University, Pullman, WA 99164 7040, USA
    J Gen Virol 81:2735-9. 2000
    ..Based on these results, it is likely that a construct expressing the 359 amino acids constituting p15 and p26 would contain epitopes capable of stimulating CTL in most horses...
  18. pmc Selection of a rare neutralization-resistant variant following passive transfer of convalescent immune plasma in equine infectious anemia virus-challenged SCID horses
    Sandra D Taylor
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Virol 84:6536-48. 2010
    ....
  19. ncbi request reprint Presentation and binding affinity of equine infectious anemia virus CTL envelope and matrix protein epitopes by an expressed equine classical MHC class I molecule
    Travis C McGuire
    Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman, WA 99164, USA
    J Immunol 171:1984-93. 2003
    ..Since MHC class I 7-6 presented both epitopes, outbred horses with this allele can be immunized with these epitopes to optimize CTL responses and evaluate their effectiveness against lentiviral challenge...
  20. ncbi request reprint Cytotoxic T lymphocytes in protection against equine infectious anemia virus
    Travis C McGuire
    Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164 7040, USA
    Anim Health Res Rev 5:271-6. 2004
    ..A possible solution to CTL escape variants is the induction of high-avidity CTL to multiple EIAV epitopes...
  21. pmc Rhodococcus equi-specific cytotoxic T lymphocytes in immune horses and development in asymptomatic foals
    Kristin M Patton
    Department of Veterinary Microbiology and Pathology, Washington State University, Bustad Hall, Room 402, Pullman, Washington 99164 7040, USA
    Infect Immun 73:2083-93. 2005
    ..However, neither antibody bound alveolar macrophages, suggesting that the R. equi-specific, major histocompatibility complex-unrestricted lysis is not restricted by a surface molecule identified by these antibodies...
  22. pmc Development of a DNA microarray for detection of expressed equine classical MHC class I sequences in a defined population
    Joshua D Ramsay
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Immunogenetics 62:633-9. 2010
    ..These results confirmed the utility of a microarray-based method for high-resolution MHC class I typing in the horse. With additional probes the array could be useful in a broader population...
  23. ncbi request reprint Identification of Rhodococcus equi lipids recognized by host cytotoxic T lymphocytes
    Seth P Harris
    Department of Veterinary Microbiology and Pathology, Washington State University, College of Veterinary Medicine, PO Box 647040, Pullman, WA 99164 7040, USA
    Microbiology 156:1836-47. 2010
    ..equi. The results also identify potential lipid antigens not previously shown to be recognized by CTLs in an important, naturally occurring actinomycete bacterial pathogen...
  24. pmc Protective effects of broadly neutralizing immunoglobulin against homologous and heterologous equine infectious anemia virus infection in horses with severe combined immunodeficiency
    Sandra D Taylor
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    J Virol 85:6814-8. 2011
    ..These results support the inclusion of broadly neutralizing antibodies in lentivirus control strategies...
  25. ncbi request reprint Selecting peptides to optimize Th1 responses to an equine lentivirus using HLA-DR binding motifs and defined HIV-1 Th peptides
    Darrilyn G Fraser
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164 7040, USA
    Immunogenetics 55:508-14. 2003
    ....

Research Grants4

  1. EIAV Control by High Avidity Rev-Specific CTL Clones
    ROBERT MEALEY; Fiscal Year: 2005
    ..The information obtained from the proposed studies should have implications for HIV-1 immunotherapy and vaccine design, where experiments of this type may be more difficult. ..
  2. EIAV Control by DNA Vaccine-Induced CTL
    ROBERT MEALEY; Fiscal Year: 2006
    ..The information obtained from the proposed studies should have implications for HIV-1 vaccine design, where experiments of this type may be more difficult. ..
  3. Lentivirus Control by CTL and Neutralizing Antibody
    ROBERT MEALEY; Fiscal Year: 2007
    ..The information obtained from the proposed studies should have implications for HIV-1 vaccine design, where experiments of this type are not possible. ..