F F Hsu

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc Structural studies on archaeal phytanyl-ether lipids isolated from membranes of extreme halophiles by linear ion-trap multiple-stage tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, United States
    Anal Chim Acta 771:73-85. 2013
  2. pmc Structural determination of glycopeptidolipids of Mycobacterium smegmatis by high-resolution multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mass Spectrom 47:1269-81. 2012
  3. ncbi request reprint Characterization of phosphatidylinositol, phosphatidylinositol-4-phosphate, and phosphatidylinositol-4,5-bisphosphate by electrospray ionization tandem mass spectrometry: a mechanistic study
    F F Hsu
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 11:986-99. 2000
  4. pmc Characterization of new metabolites from in vivo biotransformation of 2-amino-3-methylimidazo[4,5-f]quinoline in mouse by mass spectrometry
    Fong Fu Hsu
    Department of Medicine, Box 8127, Washington University School of Medicine, 660 S Euclid, St Louis, Missouri 63110, USA
    J Mass Spectrom 44:1359-68. 2009
  5. ncbi request reprint Characterization of alkylacyl, alk-1-enylacyl and lyso subclasses of glycerophosphocholine by tandem quadrupole mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Mass Spectrom 38:752-63. 2003
  6. pmc Structural characterization of sulfated steroids that activate mouse pheromone-sensing neurons
    Fong Fu Hsu
    Department of Internal Medicine, Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 47:14009-19. 2008
  7. pmc Characterization of cardiolipin from Escherichia coli by electrospray ionization with multiple stage quadrupole ion-trap mass spectrometric analysis of [M - 2H + Na]- ions
    Fong Fu Hsu
    Department of Internal Medicine, Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 17:420-9. 2006
  8. pmc Structural characterization of unsaturated glycerophospholipids by multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 19:1681-91. 2008
  9. pmc Elucidation of the double-bond position of long-chain unsaturated fatty acids by multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 19:1673-80. 2008
  10. ncbi request reprint Characterization of acylphosphatidylglycerols from Salmonella typhimurium by tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 15:1-11. 2004

Collaborators

Detail Information

Publications61

  1. pmc Structural studies on archaeal phytanyl-ether lipids isolated from membranes of extreme halophiles by linear ion-trap multiple-stage tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, United States
    Anal Chim Acta 771:73-85. 2013
    ..This mass spectrometric approach provides a simple tool to facilitate confident characterization of this unique lipid family...
  2. pmc Structural determination of glycopeptidolipids of Mycobacterium smegmatis by high-resolution multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mass Spectrom 47:1269-81. 2012
    ..These multiple-stage mass spectrometric approaches give detailed structures of GPL in complex mixtures of which the isomeric structures are difficult to define using other analytical methods...
  3. ncbi request reprint Characterization of phosphatidylinositol, phosphatidylinositol-4-phosphate, and phosphatidylinositol-4,5-bisphosphate by electrospray ionization tandem mass spectrometry: a mechanistic study
    F F Hsu
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 11:986-99. 2000
    ..These results suggest that the further deprotonated gaseous [M - 2H]2 ions of PI-P and PI-P2 are basic precursors...
  4. pmc Characterization of new metabolites from in vivo biotransformation of 2-amino-3-methylimidazo[4,5-f]quinoline in mouse by mass spectrometry
    Fong Fu Hsu
    Department of Medicine, Box 8127, Washington University School of Medicine, 660 S Euclid, St Louis, Missouri 63110, USA
    J Mass Spectrom 44:1359-68. 2009
    ..The observation of these more polar metabolites relative to IQ suggests that they may arise from a previously undescribed detoxification pathway...
  5. ncbi request reprint Characterization of alkylacyl, alk-1-enylacyl and lyso subclasses of glycerophosphocholine by tandem quadrupole mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Mass Spectrom 38:752-63. 2003
    ..Examples for identification of various subclasses of PC in biological extracts by tandem mass spectrometry applying various constant neutral loss scannings are also shown...
  6. pmc Structural characterization of sulfated steroids that activate mouse pheromone-sensing neurons
    Fong Fu Hsu
    Department of Internal Medicine, Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 47:14009-19. 2008
    ..The identification of this novel class of mammalian social signaling compounds suggests that steroid hormones, upon conjugation, assume a new biological role, conveying information about the organism's identity and physiological state...
  7. pmc Characterization of cardiolipin from Escherichia coli by electrospray ionization with multiple stage quadrupole ion-trap mass spectrometric analysis of [M - 2H + Na]- ions
    Fong Fu Hsu
    Department of Internal Medicine, Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 17:420-9. 2006
    ..This method permits the structures of CL in a mixture isolated from Escherichia coli, including species that consist of various isomers, to be unveiled in detail...
  8. pmc Structural characterization of unsaturated glycerophospholipids by multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 19:1681-91. 2008
    ....
  9. pmc Elucidation of the double-bond position of long-chain unsaturated fatty acids by multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 19:1673-80. 2008
    ..The spectrum feature dependent on the position of unsaturated double bond(s) affords unambiguous assignment of the position of double bond(s) of long-chain unsaturated fatty acids...
  10. ncbi request reprint Characterization of acylphosphatidylglycerols from Salmonella typhimurium by tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 15:1-11. 2004
    ..The identities of the minor isomeric species in the molecules can be revealed by the aforementioned structural information arising from the various ion series combined...
  11. pmc Differentiation of 1-O-alk-1'-enyl-2-acyl and 1-O-alkyl-2-acyl glycerophospholipids by multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 18:2065-73. 2007
    ..Because the [M - H](-) ions are readily formed by various GPL classes (except glycerophosphocholine) in the negative-ion mode, these mass spectrometric approaches should have broad application in the structural identification of GPLs...
  12. ncbi request reprint Studies on sulfatides by quadrupole ion-trap mass spectrometry with electrospray ionization: structural characterization and the fragmentation processes that include an unusual internal galactose residue loss and the classical charge-remote fragmentation
    Fong Fu Hsu
    Department of Internal Medicine, Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 15:536-46. 2004
    ..The fragmentation pathways of sulfatides under low-energy CAD are proposed. The pathways are supported by the MS(2)- and MS(3)-spectra of various compounds, and of their H-D exchanged analogs...
  13. pmc Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    J Am Soc Mass Spectrom 18:783-90. 2007
    ..This low-energy tandem mass spectrometric approach also permits the structures of the Arabidopsis PGs that consist of two isomeric structures to be unveiled...
  14. pmc Structural characterization of phosphatidyl-myo-inositol mannosides from Mycobacterium bovis Bacillus Calmette Guérin by multiple-stage quadrupole ion-trap mass spectrometry with electrospray ionization. I. PIMs and lyso-PIMs
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 18:466-78. 2007
    ..The combined structural information from the MS(2) and MS(3) product-ion spectra permit the complex structures of PIMs that consist of various isomers to be unveiled in detail...
  15. ncbi request reprint Structural characterization of cardiolipin by tandem quadrupole and multiple-stage quadrupole ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 16:491-504. 2005
    ..The applications of the above methods in the differentiation of cardiolipin isomers and in the identification of complex cardiolipin species consisting of multiple molecular structures are also demonstrated...
  16. pmc Structural characterization of phosphatidyl-myo-inositol mannosides from Mycobacterium bovis Bacillus Calmette Gúerin by multiple-stage quadrupole ion-trap mass spectrometry with electrospray ionization. II. Monoacyl- and diacyl-PIMs
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 18:479-92. 2007
    ..bovis Bacillus Calmette Guérin...
  17. ncbi request reprint Studies on phosphatidylserine by tandem quadrupole and multiple stage quadrupole ion-trap mass spectrometry with electrospray ionization: structural characterization and the fragmentation processes
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 16:1510-22. 2005
    ....
  18. ncbi request reprint Structural distinction among inositol phosphate isomers using high-energy and low-energy collisional-activated dissociation tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Mass Spectrom 38:447-57. 2003
    ..Under both high- and low-energy CAD, the protonated molecular species of all InsPs undergo similar fragmentation pathways, which are dominated by the consecutive losses of H(2)O, HPO(3) and H(3)PO(4)...
  19. ncbi request reprint Electrospray ionization/tandem quadrupole mass spectrometric studies on phosphatidylcholines: the fragmentation processes
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 14:352-63. 2003
    ..These pathways would clarify the mechanisms underlying the ion formations that lead to the structural characterization of PC molecules...
  20. ncbi request reprint Structural studies on ceramides as lithiated adducts by low energy collisional-activated dissociation tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 13:680-95. 2002
    ....
  21. ncbi request reprint Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization
    F F Hsu
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 11:437-49. 2000
    ..The total analysis of sphingomyelin mixtures from bovine brain, bovine erythrocytes, and chicken egg yolk is also presented...
  22. ncbi request reprint Characterization of phosphatidylethanolamine as a lithiated adduct by triple quadrupole tandem mass spectrometry with electrospray ionization
    F F Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Mass Spectrom 35:595-606. 2000
    ..Identification of GPE molecular species and specific GPE subclasses in a biological mixture by tandem mass spectrometry with various constant neutral loss scannings is also described...
  23. ncbi request reprint Charge-driven fragmentation processes in diacyl glycerophosphatidic acids upon low-energy collisional activation. A mechanistic proposal
    F F Hsu
    Department of Medicine, Mass Spectrometry Resource, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 11:797-803. 2000
    ..The differential formation of the carboxylate anions permits accurate assignment of the regiospecificity of the fatty acid substituents of GPA molecules by tandem mass spectrometry...
  24. ncbi request reprint Charge-remote and charge-driven fragmentation processes in diacyl glycerophosphoethanolamine upon low-energy collisional activation: a mechanistic proposal
    F F Hsu
    Mass Spectrometry Resource, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 11:892-9. 2000
    ..These features of tandem spectra readily identify and locate the fatty acid substituents of GPE in the glycerol backbone...
  25. pmc Electrospray ionization with low-energy collisionally activated dissociation tandem mass spectrometry of glycerophospholipids: mechanisms of fragmentation and structural characterization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, United States
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2673-95. 2009
    ..The tandem mass spectrometric approaches afford the identification of the polar head group, the fatty acid substituents and the location of the radyl groups on the glycerol backbone of all the GPLs...
  26. ncbi request reprint Structural determination of glycosphingolipids as lithiated adducts by electrospray ionization mass spectrometry using low-energy collisional-activated dissociation on a triple stage quadrupole instrument
    F F Hsu
    Mass Spectrometry Resource, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 12:61-79. 2001
    ..Constant neutral loss and precursor ion scannings to identify galactosylceramides with sphingosine or sphinganine LCB subclasses, and with specific N-2-hydroxyl fatty acid subclass in mixtures are also demonstrated...
  27. pmc Characterization of inositol phosphorylceramides from Leishmania major by tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 18:1591-604. 2007
    ....
  28. doi request reprint Structural definition of trehalose 6-monomycolates and trehalose 6,6'-dimycolates from the pathogen Rhodococcus equi by multiple-stage linear ion-trap mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Department of Internal Medicine, Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Washington University School of Medicine, 660 S Euclid, Box 8127, St Louis, MO 63110, USA
    J Am Soc Mass Spectrom 22:2160-70. 2011
    ....
  29. pmc Characterization of mycolic acids from the pathogen Rhodococcus equi by tandem mass spectrometry with electrospray ionization
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Anal Biochem 409:112-22. 2011
    ....
  30. pmc Toward total structural analysis of cardiolipins: multiple-stage linear ion-trap mass spectrometry on the [M - 2H + 3Li]+ ions
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 21:1863-9. 2010
    ..Finally, the subsequent MS(6) on the dilithiated fatty acid ions generated from MS(5) also yields feature ions that confirm the assignment...
  31. pmc Electrospray ionization multiple-stage linear ion-trap mass spectrometry for structural elucidation of triacylglycerols: assignment of fatty acyl groups on the glycerol backbone and location of double bonds
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 21:657-69. 2010
    ..They are signature ions for glycerides and the pathways leading to their formation may involve rearrangements...
  32. ncbi request reprint Characterization of ceramides by low energy collisional-activated dissociation tandem mass spectrometry with negative-ion electrospray ionization
    Fong Fu Hsu
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 13:558-70. 2002
    ....
  33. pmc Characterization of cardiolipin as the sodiated ions by positive-ion electrospray ionization with multiple stage quadrupole ion-trap mass spectrometry
    Fong Fu Hsu
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 17:1146-57. 2006
    ..However, their uses for structural characterization are less desirable because formation of the [M + Na]+ and the [M - H + 2Na]+ ions for CL is not predictable...
  34. ncbi request reprint Studies of insulin secretory responses and of arachidonic acid incorporation into phospholipids of stably transfected insulinoma cells that overexpress group VIA phospholipase A2 (iPLA2beta ) indicate a signaling rather than a housekeeping role for iPLA2b
    Z Ma
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:13198-208. 2001
    ..These studies are more consistent with a signaling than with a housekeeping role for iPLA(2)beta in insulin-secreting beta-cells...
  35. ncbi request reprint Electrospray ionization mass spectrometry analyses of nuclear membrane phospholipid loss after reperfusion of ischemic myocardium
    S D Williams
    Department of Biochemistry and Molecular Biology, Saint Louis University Health Sciences Center, St Louis, MO 63104, USA
    J Lipid Res 41:1585-95. 2000
    ..Taken toghether, these studies have demonstrated that nuclear phospholipid mass decreases after myocardial ischemia by a mechanism that involves, at least in part, phospholipolysis mediated by iPLA2...
  36. ncbi request reprint Peroxygenase metabolism of N-acetylbenzidine by prostaglandin H synthase. Formation of an N-hydroxylamine
    T V Zenser
    VA Medical Center, Division of Geriatric Medicine, St Louis University School of Medicine, St Louis, Missouri 63125, USA
    J Biol Chem 274:14850-6. 1999
    ..Results demonstrate a peroxygenase oxidation of ABZ and N'HA by PHS and suggest a stepwise oxidation of ABZ to N'-hydroxy, 4'-nitroso, and 4'-nitro products...
  37. pmc Apoptosis of insulin-secreting cells induced by endoplasmic reticulum stress is amplified by overexpression of group VIA calcium-independent phospholipase A2 (iPLA2 beta) and suppressed by inhibition of iPLA2 beta
    Sasanka Ramanadham
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, Box 8127, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Biochemistry 43:918-30. 2004
    ..These findings indicate that iPLA(2)beta participates in ER stress-induced apoptosis, a pathway that promotes beta-cell death in diabetes...
  38. ncbi request reprint Delta6-, Stearoyl CoA-, and Delta5-desaturase enzymes are expressed in beta-cells and are altered by increases in exogenous PUFA concentrations
    Sasanka Ramanadham
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, Box 8127, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Biochim Biophys Acta 1580:40-56. 2002
    ....
  39. ncbi request reprint Progressive lung disease and surfactant dysfunction with a deletion in surfactant protein C gene
    Aaron Hamvas
    Division of Newborn Medicine, Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, 1 Children s Place, St Louis, MO 63110, USA
    Am J Respir Cell Mol Biol 30:771-6. 2004
    ..We propose that a dominant negative effect on surfactant protein metabolism and function results from aggregation of misfolded proSP-C and subsequent cell injury and inflammation...
  40. pmc Islet complex lipids: involvement in the actions of group VIA calcium-independent phospholipase A(2) in beta-cells
    Sasanka Ramanadham
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110
    Diabetes 53:S179-85. 2004
    ..Detailed characterization of the iPLA(2)beta protein level reveals that beta-cells express multiple isoforms of the enzyme, and our studies involve the hypothesis that different isoforms have different functions...
  41. pmc Pancreatic islets and insulinoma cells express a novel isoform of group VIA phospholipase A2 (iPLA2 beta) that participates in glucose-stimulated insulin secretion and is not produced by alternate splicing of the iPLA2 beta transcript
    Sasanka Ramanadham
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 42:13929-40. 2003
    ....
  42. ncbi request reprint Studies of phospholipid metabolism, proliferation, and secretion of stably transfected insulinoma cells that overexpress group VIA phospholipase A2
    Z Ma
    Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Lipids 36:689-700. 2001
    ..These findings suggest that iPLA2beta plays a signaling role in beta-cells that differs from housekeeping functions in PC biosynthesis and degradation in P388D1 and CHO cells...
  43. ncbi request reprint Molecular chlorine generated by the myeloperoxidase-hydrogen peroxide-chloride system of phagocytes converts low density lipoprotein cholesterol into a family of chlorinated sterols
    S L Hazen
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 271:23080-8. 1996
    ..Our observations suggest that Cl2 generation in acidic compartments may constitute one pathway for oxidation of LDL cholesterol in the artery wall...
  44. pmc A novel mouse model of lipotoxic cardiomyopathy
    H C Chiu
    Center for Cardiovascular Research, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Clin Invest 107:813-22. 2001
    ..This novel mouse model will provide insight into the role of perturbations in myocardial lipid metabolism in the pathogenesis of inherited and acquired forms of heart failure...
  45. ncbi request reprint Methemoglobin oxidation of N-acetylbenzidine to form a sulfinamide
    T V Zenser
    Veterans Affairs Medical Center, Division of Geriatric Medicine, St Louis University School of Medicine, St Louis, Missouri, USA
    Drug Metab Dispos 29:401-6. 2001
    ..Formation of an ABZ sulfinamide conjugate with hemoglobin was demonstrated. The results demonstrate that methemoglobin can catalyze the peroxidatic formation of an ABZ sulfinamide adduct, perhaps by a diimine monocation intermediate...
  46. pmc Characterization of N-terminal processing of group VIA phospholipase A2 and of potential cleavage sites of amyloid precursor protein constructs by automated identification of signature peptides in LC/MS/MS analyses of proteolytic digests
    Haowei Song
    Washington University School of Medicine, St Louis, MO 63110, USA
    J Am Soc Mass Spectrom 15:1780-93. 2004
    ..The program has been applied to the identification of variants of proteins of biological interest, including APP cleavage products and iPLA(2)beta, and such applications demonstrate the utility of this approach...
  47. pmc A bromoenol lactone suicide substrate inactivates group VIA phospholipase A2 by generating a diffusible bromomethyl keto acid that alkylates cysteine thiols
    Haowei Song
    Medicine Department Mass Spectrometry Facility, Division of Endocrinology, Metabolism, and Lipid Research, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 45:1061-73. 2006
    ....
  48. ncbi request reprint Identification of alpha-chloro fatty aldehydes and unsaturated lysophosphatidylcholine molecular species in human atherosclerotic lesions
    Arun K Thukkani
    Department of Biochemistry and Molecular Biology, St Louis University Sciences Center, St Louis, MO, USA
    Circulation 108:3128-33. 2003
    ....
  49. pmc Sphingolipids are essential for differentiation but not growth in Leishmania
    Kai Zhang
    Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    EMBO J 22:6016-26. 2003
    ..We suggest that SL-deficient Leishmania provide a useful biological setting for tests of essential SL enzymes in other organisms where SL perturbation is lethal...
  50. ncbi request reprint Selective plasmenylcholine oxidation by hypochlorous acid: formation of lysophosphatidylcholine chlorohydrins
    Maria C Messner
    Department of Biochemistry and Molecular Biology, St Louis University Health Sciences Center, St Louis, MO 63104, USA
    Chem Phys Lipids 144:34-44. 2006
    ..Taken together, plasmenylcholine is oxidized preferentially over phosphatidylcholine and leads to the production of LPC-ClOH...
  51. ncbi request reprint Novel carbonyl and nitrile products from reactive chlorinating species attack of lysosphingolipid
    Viral V Brahmbhatt
    Department of Biochemistry and Molecular Biology, St Louis University Medical Center, St Louis, MO 63104, USA
    Chem Phys Lipids 145:72-84. 2007
    ..Thus, the present studies characterize the formation of a novel group of lipid products generated following RCS attack of lysosphingolipids...
  52. ncbi request reprint PhoP-regulated Salmonella resistance to the antimicrobial peptides magainin 2 and polymyxin B
    Yixin Shi
    Department of Molecular Microbiology, Campus Box 8230, Washington University, St Louis, MO 63110, USA
    Mol Microbiol 53:229-41. 2004
    ..Our results indicate that different PhoP-mediated modifications in lipid A are necessary for resistance to different antimicrobial peptides...
  53. pmc Identification of the lipopolysaccharide modifications controlled by the Salmonella PmrA/PmrB system mediating resistance to Fe(III) and Al(III)
    Kunihiko Nishino
    Department of Molecular Microbiology, Howard Hughes Medical Institute, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Mol Microbiol 61:645-54. 2006
    ....
  54. pmc Redirection of sphingolipid metabolism toward de novo synthesis of ethanolamine in Leishmania
    Kai Zhang
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, and Children s Hospital Oakland Research Institute, Center for Cancer Research, CA, USA
    EMBO J 26:1094-104. 2007
    ..Our results thus reveal a striking example of remodeling of the SL metabolic pathway in Leishmania...
  55. pmc Algorithm for processing raw mass spectrometric data to identify and quantitate complex lipid molecular species in mixtures by data-dependent scanning and fragment ion database searching
    Haowei Song
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 18:1848-58. 2007
    ..The current form of the algorithm can process data acquired in negative or positive ion mode for glycerophospholipid species of all major head-group classes...
  56. ncbi request reprint Myeloperoxidase-derived reactive chlorinating species from human monocytes target plasmalogens in low density lipoprotein
    Arun K Thukkani
    Department of Biochemistry and Molecular Biology, St Louis University Health Sciences Center, St Louis, Missouri 63104, USA
    J Biol Chem 278:36365-72. 2003
    ....
  57. ncbi request reprint Myeloperoxidase-derived 2-chlorohexadecanal forms Schiff bases with primary amines of ethanolamine glycerophospholipids and lysine
    Kristin R Wildsmith
    Department of Biochemistry and Molecular Biology, St Louis University Health Sciences Center, St Louis, MO 63104, United States
    Chem Phys Lipids 139:157-70. 2006
    ..These results show novel Schiff base adducts of alpha-Cl-FALDs with primary amines, which may represent an important fate of alpha-Cl-FALDs...
  58. ncbi request reprint Effectors of rapid homeostatic responses of endoplasmic reticulum cholesterol and 3-hydroxy-3-methylglutaryl-CoA reductase
    Yvonne Lange
    Department of Pathology, Rush University Medical Center, 1653 W Congress Parkway, Chicago, IL 60612, USA
    J Biol Chem 283:1445-55. 2008
    ..On the other hand, 27-hydroxycholesterol, rather than cholesterol itself or biosynthetic precursors of cholesterol, stimulates the rapid inactivation of HMGR in response to high levels of cholesterol...
  59. ncbi request reprint Reactive brominating species produced by myeloperoxidase target the vinyl ether bond of plasmalogens: disparate utilization of sodium halides in the production of alpha-halo fatty aldehydes
    Carolyn J Albert
    Department of Biochemistry and Molecular Biology, St Louis University Health Sciences Center, St Louis, Missouri 63104, USA
    J Biol Chem 277:4694-703. 2002
    ....
  60. ncbi request reprint Reactive chlorinating species produced during neutrophil activation target tissue plasmalogens: production of the chemoattractant, 2-chlorohexadecanal
    Arun K Thukkani
    Department of Biochemistry, St Louis University Health Sciences Center, St Louis, Missouri 63104, USA
    J Biol Chem 277:3842-9. 2002
    ....
  61. ncbi request reprint Ionic-liquid matrices for improved analysis of phospholipids by MALDI-TOF mass spectrometry
    Ying L Li
    Department of Chemistry, Washington University, St Louis, Missouri 63130, USA
    J Am Soc Mass Spectrom 16:679-82. 2005
    ..The ionization products are comparable to those with 2,5-DHB although the use of ILMs gives a stronger tendency to produce alkali-metal-ion adducts and a lower extent of prompt fragmentation...