Xianlin Han

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi request reprint CD36 deficiency rescues lipotoxic cardiomyopathy
    John Yang
    Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    Circ Res 100:1208-17. 2007
  2. ncbi request reprint Plasmalogen deficiency in early Alzheimer's disease subjects and in animal models: molecular characterization using electrospray ionization mass spectrometry
    X Han
    Division of Bioorganic Chemistry, Department of Internal Medicine, Center for the Study of Nervous System Injury, Washington University School of Medicine, St Louis, Missouri, USA
    J Neurochem 77:1168-80. 2001
  3. ncbi request reprint Cerebrospinal fluid sulfatide is decreased in subjects with incipient dementia
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, and the Departments of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Ann Neurol 54:115-9. 2003
  4. ncbi request reprint Novel role for apolipoprotein E in the central nervous system. Modulation of sulfatide content
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:8043-51. 2003
  5. ncbi request reprint Substantial sulfatide deficiency and ceramide elevation in very early Alzheimer's disease: potential role in disease pathogenesis
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurochem 82:809-18. 2002
  6. ncbi request reprint Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry
    X Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Biochem 295:88-100. 2001
  7. pmc Genetic ablation of calcium-independent phospholipase A2gamma prevents obesity and insulin resistance during high fat feeding by mitochondrial uncoupling and increased adipocyte fatty acid oxidation
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 285:36495-510. 2010
  8. pmc The transcriptional coactivator PGC-1alpha is essential for maximal and efficient cardiac mitochondrial fatty acid oxidation and lipid homeostasis
    John J Lehman
    Center for Cardiovascular Research, Washington Univ School of Medicine, St Louis, MO 63110, USA
    Am J Physiol Heart Circ Physiol 295:H185-96. 2008
  9. ncbi request reprint Dramatic accumulation of triglycerides and precipitation of cardiac hemodynamic dysfunction during brief caloric restriction in transgenic myocardium expressing human calcium-independent phospholipase A2gamma
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:9216-27. 2007
  10. pmc Nuclear receptors PPARbeta/delta and PPARalpha direct distinct metabolic regulatory programs in the mouse heart
    Eileen M Burkart
    Center for Cardiovascular Research, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 117:3930-9. 2007

Collaborators

Detail Information

Publications87

  1. ncbi request reprint CD36 deficiency rescues lipotoxic cardiomyopathy
    John Yang
    Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    Circ Res 100:1208-17. 2007
    ....
  2. ncbi request reprint Plasmalogen deficiency in early Alzheimer's disease subjects and in animal models: molecular characterization using electrospray ionization mass spectrometry
    X Han
    Division of Bioorganic Chemistry, Department of Internal Medicine, Center for the Study of Nervous System Injury, Washington University School of Medicine, St Louis, Missouri, USA
    J Neurochem 77:1168-80. 2001
    ....
  3. ncbi request reprint Cerebrospinal fluid sulfatide is decreased in subjects with incipient dementia
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, and the Departments of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Ann Neurol 54:115-9. 2003
    ..The cerebrospinal fluid ST to phosphatidylinositol ratio may be a very useful biomarker for the earliest clinical stage of Alzheimer's disease...
  4. ncbi request reprint Novel role for apolipoprotein E in the central nervous system. Modulation of sulfatide content
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:8043-51. 2003
    ..This novel role for apoE in the CNS may provide new insights into the connection of apoE with Alzheimer's disease and poor recovery after brain injury...
  5. ncbi request reprint Substantial sulfatide deficiency and ceramide elevation in very early Alzheimer's disease: potential role in disease pathogenesis
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurochem 82:809-18. 2002
    ..The findings demonstrate that a marked decrease in sulfatides is associated with AD pathology even in subjects with very mild dementia and that these changes may be linked with early events in the pathological process of AD...
  6. ncbi request reprint Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry
    X Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Biochem 295:88-100. 2001
    ..An algorithm was developed which identifies sensitivity factors, thereby allowing the rapid quantitation and molecular species fingerprinting of TAG molecular species directly from chloroform extracts of biological samples...
  7. pmc Genetic ablation of calcium-independent phospholipase A2gamma prevents obesity and insulin resistance during high fat feeding by mitochondrial uncoupling and increased adipocyte fatty acid oxidation
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 285:36495-510. 2010
    ....
  8. pmc The transcriptional coactivator PGC-1alpha is essential for maximal and efficient cardiac mitochondrial fatty acid oxidation and lipid homeostasis
    John J Lehman
    Center for Cardiovascular Research, Washington Univ School of Medicine, St Louis, MO 63110, USA
    Am J Physiol Heart Circ Physiol 295:H185-96. 2008
    ..Overall, these results demonstrate that PGC-1alpha is essential for the maintenance of maximal, efficient cardiac mitochondrial fatty acid oxidation, ATP synthesis, and myocardial lipid homeostasis...
  9. ncbi request reprint Dramatic accumulation of triglycerides and precipitation of cardiac hemodynamic dysfunction during brief caloric restriction in transgenic myocardium expressing human calcium-independent phospholipase A2gamma
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:9216-27. 2007
    ....
  10. pmc Nuclear receptors PPARbeta/delta and PPARalpha direct distinct metabolic regulatory programs in the mouse heart
    Eileen M Burkart
    Center for Cardiovascular Research, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 117:3930-9. 2007
    ....
  11. pmc Genetic ablation of calcium-independent phospholipase A2gamma leads to alterations in mitochondrial lipid metabolism and function resulting in a deficient mitochondrial bioenergetic phenotype
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:34611-22. 2007
    ..We conclude that iPLA(2)gamma is essential for maintaining efficient bioenergetic mitochondrial function through tailoring mitochondrial membrane lipid metabolism and composition...
  12. pmc Apolipoprotein E mediates sulfatide depletion in animal models of Alzheimer's disease
    Hua Cheng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Neurobiol Aging 31:1188-96. 2010
    ..Collectively, through different animal models of AD, this study provides evidence for an identified biochemical mechanism that may be responsible for the sulfatide depletion at the earliest stages of AD...
  13. pmc Alterations in lipid homeostasis of mouse dorsal root ganglia induced by apolipoprotein E deficiency: a shotgun lipidomics study
    Hua Cheng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurochem 101:57-76. 2007
    ....
  14. pmc Shotgun lipidomics reveals the temporally dependent, highly diversified cardiolipin profile in the mammalian brain: temporally coordinated postnatal diversification of cardiolipin molecular species with neuronal remodeling
    Hua Cheng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 47:5869-80. 2008
    ..Collectively, these results provide a new perspective on the complexity of CL in neuronal signaling, mitochondrial bioenergetics, and apoptosis...
  15. ncbi request reprint A regulatory role for 1-acylglycerol-3-phosphate-O-acyltransferase 2 in adipocyte differentiation
    Sarah E Gale
    Center for Cardiovascular Research and Department of Internal Medicine, School of Medicine, Washington University, St Louis, Missouri 63110 1010, USA
    J Biol Chem 281:11082-9. 2006
    ..These findings underscore the importance of an AGPAT2-mediated metabolic pathway in adipocyte differentiation...
  16. ncbi request reprint ABCA1 is required for normal central nervous system ApoE levels and for lipidation of astrocyte-secreted apoE
    Suzanne E Wahrle
    Program in Neurosciences, Washington University, St Louis, Missouri 63110, USA
    J Biol Chem 279:40987-93. 2004
    ..Since apoE isoforms and levels strongly influence Alzheimer's disease pathology and risk, these data suggest that ABCA1 may be a novel therapeutic target...
  17. pmc Dysfunctional cardiac mitochondrial bioenergetic, lipidomic, and signaling in a murine model of Barth syndrome
    Michael A Kiebish
    Division of Bioorganic Chemistry and Molecular Pharmacology, Departments of Medicine, Washington University School of Medicine, St Louis, MO 63110 and
    J Lipid Res 54:1312-25. 2013
    ..Collectively, we identified specific lipidomic, bioenergetic, and signaling alterations in a murine model that parallel those of Barth syndrome thereby providing novel insights into the pathophysiology of this debilitating disease...
  18. ncbi request reprint The highly selective production of 2-arachidonoyl lysophosphatidylcholine catalyzed by purified calcium-independent phospholipase A2gamma: identification of a novel enzymatic mediator for the generation of a key branch point intermediate in eicosanoid sig
    Wei Yan
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26669-79. 2005
    ....
  19. pmc Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of cellular glycerophospholipids enabled by multiplexed solvent dependent analyte-matrix interactions
    Gang Sun
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Chem 80:7576-85. 2008
    ....
  20. ncbi request reprint Shotgun lipidomics identifies cardiolipin depletion in diabetic myocardium linking altered substrate utilization with mitochondrial dysfunction
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 44:16684-94. 2005
    ....
  21. pmc Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1α-/- mouse cortex: a potential lipidomic role of PGC-1α in neurodegeneration
    Michael A Kiebish
    Division of Bioorganic Chemistry and Molecular Pharmacology and Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 53:273-81. 2012
    ....
  22. pmc A critical role for PPARalpha-mediated lipotoxicity in the pathogenesis of diabetic cardiomyopathy: modulation by dietary fat content
    Brian N Finck
    Department of Medicine, Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 100:1226-31. 2003
    ..These results link dysregulation of the PPARalpha gene regulatory pathway to cardiac dysfunction in the diabetic and provide a rationale for serum lipid-lowering strategies in the treatment of diabetic cardiomyopathy...
  23. pmc Myocardial regulation of lipidomic flux by cardiolipin synthase: setting the beat for bioenergetic efficiency
    Michael A Kiebish
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 287:25086-97. 2012
    ....
  24. pmc Genetic ablation of calcium-independent phospholipase A2{gamma} leads to alterations in hippocampal cardiolipin content and molecular species distribution, mitochondrial degeneration, autophagy, and cognitive dysfunction
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:35632-44. 2009
    ....
  25. ncbi request reprint Disruption of endoplasmic reticulum structure and integrity in lipotoxic cell death
    Nica M Borradaile
    Center for Cardiovascular Research, Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:2726-37. 2006
    ....
  26. pmc Alterations in myocardial cardiolipin content and composition occur at the very earliest stages of diabetes: a shotgun lipidomics study
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 46:6417-28. 2007
    ....
  27. ncbi request reprint Accumulation of long-chain acylcarnitine and 3-hydroxy acylcarnitine molecular species in diabetic myocardium: identification of alterations in mitochondrial fatty acid processing in diabetic myocardium by shotgun lipidomics
    Xiong Su
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 44:5234-45. 2005
    ....
  28. pmc Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics
    Michael A Kiebish
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 51:2153-70. 2010
    ..We believe this study provides a foundation to investigate cardiolipin metabolism and bioenergetic homeostasis in normal and disease states...
  29. pmc Epidermal growth factor receptors are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: a shotgun lipidomics study
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26796-804. 2005
    ....
  30. ncbi request reprint Sequential ordered fatty acid alpha oxidation and Delta9 desaturation are major determinants of lipid storage and utilization in differentiating adipocytes
    Xiong Su
    Department of Chemistry, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 43:5033-44. 2004
    ..Collectively, these results underscore the role of peroxisomal fatty acid processing as an important determinant of the metabolic fate of fatty acids in the differentiating adipocyte...
  31. pmc Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix
    Hua Cheng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis MO 63110, USA
    J Lipid Res 51:1599-609. 2010
    ....
  32. pmc Shotgun lipidomics of cardiolipin molecular species in lipid extracts of biological samples
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:864-79. 2006
    ....
  33. pmc Shotgun metabolomics approach for the analysis of negatively charged water-soluble cellular metabolites from mouse heart tissue
    Gang Sun
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Anal Chem 79:6629-40. 2007
    ..Collectively, these results identify a multiplexed MALDI-TOF/TOF MS approach for analysis of negatively charged metabolites in mammalian tissues...
  34. pmc Shotgun lipidomics identifies a paired rule for the presence of isomeric ether phospholipid molecular species
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 2:e1368. 2007
    ..g., heart and the brain) that play important roles in cellular function. Alterations of ether phospholipid molecular species contents are associated with a number of genetic disorders and human diseases...
  35. pmc Lipin 2 is a liver-enriched phosphatidate phosphohydrolase enzyme that is dynamically regulated by fasting and obesity in mice
    Matthew C Gropler
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:6763-72. 2009
    ..Collectively, these data suggest that lipin 2 plays an important role as a hepatic PAP-1 enzyme...
  36. ncbi request reprint Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy
    Hsiu Chiang Chiu
    Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    Circ Res 96:225-33. 2005
    ..Moreover, the MHC-FATP model supports a role for FATPs in FFA import into the heart in vivo...
  37. ncbi request reprint Decreased hepatic triglyceride accumulation and altered fatty acid uptake in mice with deletion of the liver fatty acid-binding protein gene
    Elizabeth P Newberry
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:51664-72. 2003
    ..These data point to an inducible defect in fatty acid utilization in fasted L-Fabp-/- mice that involves targeting of substrate for use in triglyceride metabolism...
  38. pmc Direct quantitation of psychosine from alkaline-treated lipid extracts with a semi-synthetic internal standard
    Xuntian Jiang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 50:162-72. 2009
    ....
  39. ncbi request reprint CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine
    Fatiha Nassir
    Department of Medicine, Division of Nutritional Science, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:19493-501. 2007
    ..The findings support the role of CD36 in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, whereas CD36-independent mechanisms predominate in distal segments...
  40. ncbi request reprint Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics
    Xianlin Han
    Division of Bioorganic Chemistry, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 44:1071-9. 2003
    ..We believe that ESI/MS-facilitated lipidomics has now opened a critical door that will greatly increase our understanding of human disease...
  41. pmc Systematic analysis of choline-containing phospholipids using multi-dimensional mass spectrometry-based shotgun lipidomics
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, Box 8020, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2924-36. 2009
    ....
  42. ncbi request reprint Functional lipidomics: the roles of specialized lipids and lipid-protein interactions in modulating neuronal function
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8020, St Louis, MO 63110, USA
    Prostaglandins Other Lipid Mediat 77:52-64. 2005
    ..This review summarizes the advances in our understanding of the types of lipids and phospholipases in the brain and the role of functional lipidomics in increasing our chemical understanding of complex neuronal processes...
  43. ncbi request reprint Caloric restriction results in phospholipid depletion, membrane remodeling, and triacylglycerol accumulation in murine myocardium
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, and Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 43:15584-94. 2004
    ....
  44. pmc The cardiac phenotype induced by PPARalpha overexpression mimics that caused by diabetes mellitus
    Brian N Finck
    Center for Cardiovascular Research, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 109:121-30. 2002
    ....
  45. pmc Alkaline methanolysis of lipid extracts extends shotgun lipidomics analyses to the low-abundance regime of cellular sphingolipids
    Xuntian Jiang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Biochem 371:135-45. 2007
    ..Accordingly, through utilization of these facile enrichment techniques, direct penetrance into the sphingolipidomes has been greatly extended, facilitating new insights into their metabolism and signaling functions in biological systems...
  46. ncbi request reprint Cardiac ischemia activates calcium-independent phospholipase A2beta, precipitating ventricular tachyarrhythmias in transgenic mice: rescue of the lethal electrophysiologic phenotype by mechanism-based inhibition
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:22231-6. 2003
    ....
  47. pmc Sulfatides facilitate apolipoprotein E-mediated amyloid-beta peptide clearance through an endocytotic pathway
    Youchun Zeng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurochem 106:1275-86. 2008
    ....
  48. ncbi request reprint Purification of recombinant human cPLA2 gamma and identification of C-terminal farnesylation, proteolytic processing, and carboxymethylation by MALDI-TOF-TOF analysis
    Christopher M Jenkins
    Division of Bioorganic Chemistry, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 42:11798-807. 2003
    ....
  49. pmc Abundance of triacylglycerols in ganglia and their depletion in diabetic mice: implications for the role of altered triacylglycerols in diabetic neuropathy
    Hua Cheng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurochem 97:1288-300. 2006
    ..Collectively, these results indicate that TAG is an important component of ganglia and may potentially contribute to pathological alterations in peripheral neuronal function in diabetic neuropathy...
  50. pmc Side chain oxygenated cholesterol regulates cellular cholesterol homeostasis through direct sterol-membrane interactions
    Sarah E Gale
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:1755-64. 2009
    ..These studies implicate oxysterol-membrane interactions in acute regulation of sterol homeostatic responses and provide new insights into the mechanism through which oxysterols regulate cellular cholesterol balance...
  51. ncbi request reprint Submicromolar concentrations of palmitoyl-CoA specifically thioesterify cysteine 244 in glyceraldehyde-3-phosphate dehydrogenase inhibiting enzyme activity: a novel mechanism potentially underlying fatty acid induced insulin resistance
    Jingyue Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 44:11903-12. 2005
    ....
  52. pmc The pathogenic implication of abnormal interaction between apolipoprotein E isoforms, amyloid-beta peptides, and sulfatides in Alzheimer's disease
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, Campus Box 8020, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Mol Neurobiol 41:97-106. 2010
    ..Collectively, our studies clearly provide a link between apoE, Abeta, and sulfatides in AD and establish a foundation for the development of effective therapeutic interventions for AD...
  53. ncbi request reprint Lipid alterations in the earliest clinically recognizable stage of Alzheimer's disease: implication of the role of lipids in the pathogenesis of Alzheimer's disease
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Curr Alzheimer Res 2:65-77. 2005
    ..Collectively, this review will provide an overview of the lipid alterations in Alzheimer's disease and the relationship of these lipid alterations with the development of AD pathogenesis...
  54. ncbi request reprint Lipidomics in diabetes and the metabolic syndrome
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Methods Enzymol 433:73-90. 2007
    ..Through the use and practice of shotgun lipidomics, new insights into the cardiovascular pathobiology manifest in diabetes and the metabolic syndrome can be accrued...
  55. pmc A practical approach for determination of mass spectral baselines
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Am Soc Mass Spectrom 22:2090-9. 2011
    ....
  56. pmc Neuronal LRP1 knockout in adult mice leads to impaired brain lipid metabolism and progressive, age-dependent synapse loss and neurodegeneration
    Qiang Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 30:17068-78. 2010
    ..Together, these studies support a critical role for LRP1 in maintaining brain lipid homeostasis and associated synaptic and neuronal integrity, and provide important insights into the pathophysiological mechanisms in AD...
  57. pmc Identification and quantitation of unsaturated fatty acid isomers by electrospray ionization tandem mass spectrometry: a shotgun lipidomics approach
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Chem 83:4243-50. 2011
    ..We believe that this approach should further advance the lipidomic power for identification of the biochemical mechanisms underlying metabolic diseases...
  58. ncbi request reprint Factors influencing the electrospray intrasource separation and selective ionization of glycerophospholipids
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 17:264-74. 2006
    ....
  59. pmc Automated lipid identification and quantification by multidimensional mass spectrometry-based shotgun lipidomics
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Chem 81:4356-68. 2009
    ..Accordingly, the development of this program should greatly accelerate high-throughput analysis of lipids using MDMS-based shotgun lipidomics...
  60. doi request reprint Qualitative and quantitative comparison of brand name and generic protein pharmaceuticals using isotope tags for relative and absolute quantification and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry
    Hongping Ye
    U S Food and Drug Administration, Center for Drug Evaluation and Research, Division of Pharmaceutical Analysis DPA, St Louis, MO 63101, USA
    Anal Biochem 400:46-55. 2010
    ..The iTRAQ and global internal standard technology (GIST) methods were compared, and the iTRAQ method provided both higher sequence coverage and enhanced signal intensity...
  61. pmc Lysosomal phospholipase A2 and phospholipidosis
    Miki Hiraoka
    Nephrology Division, Department of Internal Medicine, University of Michigan, Room 1560 MSRB II, 1150 West Medical Center Dr, Ann Arbor, MI 48103 0676, USA
    Mol Cell Biol 26:6139-48. 2006
    ..Thus, a deficiency of lysosomal phospholipase A2 results in foam cell formation, surfactant lipid accumulation, splenomegaly, and phospholipidosis in mice...
  62. ncbi request reprint Cellular catabolism of lipid poor apolipoprotein E via cell surface LDL receptor-related protein
    Masaaki Narita
    Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, St Louis, Missouri 63110, USA
    J Biochem 132:743-9. 2002
    ....
  63. ncbi request reprint Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Anal Biochem 330:317-31. 2004
    ..This new integrated platform provides a robust foundation for the automated analysis of multidimensional mass spectra to advance the level of analytical scripting in lipidomics to a new plateau...
  64. pmc Amyloid-beta peptide induces oligodendrocyte death by activating the neutral sphingomyelinase-ceramide pathway
    Jiunn Tay Lee
    Center for the Study of Nervous System Injury, Dept of Neurology, Washington University School of Medicine, 660 S Euclid Ave, Box 8111, St Louis, MO 63110, USA
    J Cell Biol 164:123-31. 2004
    ..These results suggest that Abeta induces OLG death by activating the nSMase-ceramide cascade via an oxidative mechanism...
  65. pmc Cholesterol suppresses cellular TGF-beta responsiveness: implications in atherogenesis
    Chun Lin Chen
    Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 S Grand Blvd, St Louis, MO 63104, USA
    J Cell Sci 120:3509-21. 2007
    ..These results suggest that high cholesterol contributes to atherogenesis, at least in part, by suppressing TGF-beta responsiveness in vascular cells...
  66. pmc Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 46:1548-60. 2005
    ....
  67. pmc Multi-dimensional mass spectrometry-based shotgun lipidomics and the altered lipids at the mild cognitive impairment stage of Alzheimer's disease
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Biochim Biophys Acta 1801:774-83. 2010
    ..Collectively, the results obtained from lipidomic analyses of brain samples provide important insights into the biochemical mechanisms underlying the pathogenesis of Alzheimer's disease...
  68. ncbi request reprint Characterization and direct quantitation of cerebroside molecular species from lipid extracts by shotgun lipidomics
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 46:163-75. 2005
    ..This method was demonstrated to have a greater than 1,000-fold linear dynamic range in the low concentration region; therefore, it should have a wide range of applications in studies of the cellular sphingolipid lipidome...
  69. ncbi request reprint Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mass Spectrom Rev 24:367-412. 2005
    ....
  70. ncbi request reprint Shotgun lipidomics: multidimensional MS analysis of cellular lipidomes
    Xianlin Han
    Washington University School of Medicine, Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, St Louis, Missouri 63110, USA
    Expert Rev Proteomics 2:253-64. 2005
    ..Thus, understanding the biochemical mechanisms underlying lipid-mediated disease states will be greatly facilitated by the power of shotgun lipidomics...
  71. ncbi request reprint An update on lipidomics: progress and application in biomarker and drug development
    Xianlin Han
    Washington University School of Medicine, Department of Internal Medicine, St Louis, MO 63110, USA
    Curr Opin Mol Ther 9:586-91. 2007
    ..An update of technical developments within the field of lipidomics from 2006 to the present is provided, along with a brief discussion of its applications in biomarker and drug development...
  72. pmc Neurolipidomics: challenges and developments
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, Box 8020, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Front Biosci 12:2601-15. 2007
    ..Accordingly, with neurolipidomics, our understanding of the complexities of the nervous system will be undoubtedly accelerated, as many mysteries are resolved...
  73. pmc Potential mechanisms contributing to sulfatide depletion at the earliest clinically recognizable stage of Alzheimer's disease: a tale of shotgun lipidomics
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurochem 103:171-9. 2007
    ..Collectively, the results obtained from lipidomic analyses of brain samples provide important insights into the biochemical mechanisms underlying AD pathogenesis...
  74. pmc Shotgun lipidomics of neutral lipids as an enabling technology for elucidation of lipid-related diseases
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Departments of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Am J Physiol Endocrinol Metab 297:E297-303. 2009
    ..Through exploiting the high information content inherent in shotgun lipidomics, this technology promises to greatly facilitate advances in our understanding of alterations in neutral lipid metabolism in health and disease...
  75. pmc Microfluidics-based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi-dimensional mass spectrometry: development of an automated high-throughput platform f
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Rapid Commun Mass Spectrom 22:2115-24. 2008
    ....
  76. pmc Lipidomics at the interface of structure and function in systems biology
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Chem Biol 18:284-91. 2011
    ..Through both targeted and nontargeted investigations, lipidomics has already revealed the chemical mechanisms underlying many lipid-related disease states...
  77. ncbi request reprint Lipid rafts are enriched in arachidonic acid and plasmenylethanolamine and their composition is independent of caveolin-1 expression: a quantitative electrospray ionization/mass spectrometric analysis
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:2075-88. 2002
    ..These data provide insight into the structure of lipid rafts and identify potential new roles for these domains in signal transduction...
  78. ncbi request reprint Identification of hepatic peroxisomal phospholipase A(2) and characterization of arachidonic acid-containing choline glycerophospholipids in hepatic peroxisomes
    Jingyue Yang
    Department of Chemistry, Washington University, Campus Box 1134, One Brookings Drive, St Louis, MO 63130, USA
    FEBS Lett 546:247-50. 2003
    ....
  79. pmc Triglyceride accumulation protects against fatty acid-induced lipotoxicity
    Laura L Listenberger
    Center for Cardiovascular Research, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110 1010, USA
    Proc Natl Acad Sci U S A 100:3077-82. 2003
    ..Our findings support a model of cellular lipid metabolism in which unsaturated fatty acids serve a protective function against lipotoxicity though promotion of triglyceride accumulation...
  80. pmc Characterization and direct quantitation of sphingoid base-1-phosphates from lipid extracts: a shotgun lipidomics approach
    Xuntian Jiang
    Department of Medicine, Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:1865-73. 2006
    ....
  81. ncbi request reprint Characterization and direct quantitation of ceramide molecular species from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Biochem 302:199-212. 2002
    ....
  82. ncbi request reprint Identification of calcium-independent phospholipase A2 (iPLA2) beta, and not iPLA2gamma, as the mediator of arginine vasopressin-induced arachidonic acid release in A-10 smooth muscle cells. Enantioselective mechanism-based discrimination of mammalian iPL
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 277:32807-14. 2002
    ....
  83. pmc Effects of human apolipoprotein E isoforms on the amyloid beta-protein concentration and lipid composition in brain low-density membrane domains
    Maho Morishima-Kawashima
    Department of Neuropathology, Faculty of Medicine, University of Tokyo, Tokyo, Japan
    J Neurochem 101:949-58. 2007
    ..These data show that the apoE isoform-specific effects on the A beta concentration in LDM domains do not occur in KI mouse models...
  84. doi request reprint Ceramide is responsible for the failure of compensatory nerve sprouting in apolipoprotein E knock-out mice
    Dusica Maysinger
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada
    J Neurosci 28:7891-9. 2008
    ..Nontoxic ceramide modulators might usefully promote sprouting and circuitry repair in neurodegenerative disorders in which ceramide species are perturbed, adding to the benefits of reducing ceramide-induced neuronal apoptosis...
  85. pmc Lipidomic analysis and electron transport chain activities in C57BL/6J mouse brain mitochondria
    Michael A Kiebish
    Biology Department, Boston College, Chestnut Hill, Massachusetts 02467, USA
    J Neurochem 106:299-312. 2008
    ..NS and Syn mitochondrial lipidomic heterogeneity could influence energy metabolism, which may contribute to metabolic compartmentation of the brain...
  86. pmc Differential regulation of sphingomyelin synthesis and catabolism in oligodendrocytes and neurons
    John P Kilkus
    Department of Pediatrics, University of Chicago, Chicago, Illinois, USA
    J Neurochem 106:1745-57. 2008
    ..Thus, SMS rather than SMases may be more important for sphingolipid homeostasis in oligodendrocytes, whereas the reverse may be true for neurons...
  87. pmc Diabetes alters sphingolipid metabolism in the retina: a potential mechanism of cell death in diabetic retinopathy
    Todd E Fox
    Department of Pharmacology H078, Penn State College of Medicine, Hershey, PA 17033, USA
    Diabetes 55:3573-80. 2006
    ..Thus, dysfunctional glycosphingolipid metabolism may contribute to metabolic stress in diabetes, and therapeutic strategies to restore normal sphingolipid metabolism may be a viable approach for treatment of diabetic retinopathy...