Richard Gross

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi Shotgun lipidomics of cardiolipin molecular species in lipid extracts of biological samples
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:864-79. 2006
  2. ncbi The transcriptional coactivator PGC-1alpha is essential for maximal and efficient cardiac mitochondrial fatty acid oxidation and lipid homeostasis
    John J Lehman
    Center for Cardiovascular Research, Washington Univ School of Medicine, St Louis, MO 63110, USA
    Am J Physiol Heart Circ Physiol 295:H185-96. 2008
  3. ncbi The human Kv1.1 channel is palmitoylated, modulating voltage sensing: Identification of a palmitoylation consensus sequence
    Rose A Gubitosi-Klug
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 102:5964-8. 2005
  4. ncbi Functional lipidomics: the roles of specialized lipids and lipid-protein interactions in modulating neuronal function
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8020, St Louis, MO 63110, USA
    Prostaglandins Other Lipid Mediat 77:52-64. 2005
  5. ncbi Shotgun lipidomics of neutral lipids as an enabling technology for elucidation of lipid-related diseases
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Departments of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Am J Physiol Endocrinol Metab 297:E297-303. 2009
  6. ncbi Lipidomics at the interface of structure and function in systems biology
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Chem Biol 18:284-91. 2011
  7. ncbi The cardiac phenotype induced by PPARalpha overexpression mimics that caused by diabetes mellitus
    Brian N Finck
    Center for Cardiovascular Research, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Clin Invest 109:121-30. 2002
  8. ncbi Dramatic accumulation of triglycerides and precipitation of cardiac hemodynamic dysfunction during brief caloric restriction in transgenic myocardium expressing human calcium-independent phospholipase A2gamma
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:9216-27. 2007
  9. ncbi Caloric restriction results in phospholipid depletion, membrane remodeling, and triacylglycerol accumulation in murine myocardium
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, and Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Biochemistry 43:15584-94. 2004
  10. ncbi Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:48968-75. 2004

Research Grants

  1. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 1992
  2. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 2005
  3. MEMBRANE MECHANISMS MEDIATING ALCOHOL INDUCED PATHOLOGY
    Richard Gross; Fiscal Year: 2001
  4. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 2007
  5. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 2000
  6. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard W Gross; Fiscal Year: 2010

Collaborators

Detail Information

Publications62

  1. ncbi Shotgun lipidomics of cardiolipin molecular species in lipid extracts of biological samples
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 47:864-79. 2006
    ....
  2. ncbi The transcriptional coactivator PGC-1alpha is essential for maximal and efficient cardiac mitochondrial fatty acid oxidation and lipid homeostasis
    John J Lehman
    Center for Cardiovascular Research, Washington Univ School of Medicine, St Louis, MO 63110, USA
    Am J Physiol Heart Circ Physiol 295:H185-96. 2008
    ..Overall, these results demonstrate that PGC-1alpha is essential for the maintenance of maximal, efficient cardiac mitochondrial fatty acid oxidation, ATP synthesis, and myocardial lipid homeostasis...
  3. ncbi The human Kv1.1 channel is palmitoylated, modulating voltage sensing: Identification of a palmitoylation consensus sequence
    Rose A Gubitosi-Klug
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 102:5964-8. 2005
    ....
  4. ncbi Functional lipidomics: the roles of specialized lipids and lipid-protein interactions in modulating neuronal function
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8020, St Louis, MO 63110, USA
    Prostaglandins Other Lipid Mediat 77:52-64. 2005
    ..This review summarizes the advances in our understanding of the types of lipids and phospholipases in the brain and the role of functional lipidomics in increasing our chemical understanding of complex neuronal processes...
  5. ncbi Shotgun lipidomics of neutral lipids as an enabling technology for elucidation of lipid-related diseases
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Departments of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Am J Physiol Endocrinol Metab 297:E297-303. 2009
    ..Through exploiting the high information content inherent in shotgun lipidomics, this technology promises to greatly facilitate advances in our understanding of alterations in neutral lipid metabolism in health and disease...
  6. ncbi Lipidomics at the interface of structure and function in systems biology
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Chem Biol 18:284-91. 2011
    ..Through both targeted and nontargeted investigations, lipidomics has already revealed the chemical mechanisms underlying many lipid-related disease states...
  7. ncbi The cardiac phenotype induced by PPARalpha overexpression mimics that caused by diabetes mellitus
    Brian N Finck
    Center for Cardiovascular Research, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Clin Invest 109:121-30. 2002
    ....
  8. ncbi Dramatic accumulation of triglycerides and precipitation of cardiac hemodynamic dysfunction during brief caloric restriction in transgenic myocardium expressing human calcium-independent phospholipase A2gamma
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:9216-27. 2007
    ....
  9. ncbi Caloric restriction results in phospholipid depletion, membrane remodeling, and triacylglycerol accumulation in murine myocardium
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, and Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Biochemistry 43:15584-94. 2004
    ....
  10. ncbi Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:48968-75. 2004
    ....
  11. ncbi CD36 deficiency rescues lipotoxic cardiomyopathy
    John Yang
    Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    Circ Res 100:1208-17. 2007
    ....
  12. ncbi Decreased hepatic triglyceride accumulation and altered fatty acid uptake in mice with deletion of the liver fatty acid-binding protein gene
    Elizabeth P Newberry
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:51664-72. 2003
    ..These data point to an inducible defect in fatty acid utilization in fasted L-Fabp-/- mice that involves targeting of substrate for use in triglyceride metabolism...
  13. ncbi Highly selective hydrolysis of fatty acyl-CoAs by calcium-independent phospholipase A2beta. Enzyme autoacylation and acyl-CoA-mediated reversal of calmodulin inhibition of phospholipase A2 activity
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Biol Chem 281:15615-24. 2006
    ....
  14. ncbi Calcium-independent phospholipase A(2)-derived arachidonic acid is essential for endothelium-dependent relaxation by acetylcholine
    Hélène C Seegers
    Department of Anesthesiology, Department of Medicine, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    J Pharmacol Exp Ther 302:918-23. 2002
    ..05). The results of the present study indicate that iPLA(2)-produced AA plays an essential role in ACh-mediated endothelium-dependent relaxation in rat mesenteric resistance arteries...
  15. ncbi Genetic and pharmacologic evidence that calcium-independent phospholipase A2beta regulates virus-induced inducible nitric-oxide synthase expression by macrophages
    Jason M Moran
    The Edward A Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 280:28162-8. 2005
    ..These findings provide evidence for a signaling role for iPLA2beta in virus-induced iNOS expression by macrophages...
  16. ncbi Genetic ablation of calcium-independent phospholipase A(2)beta causes hypercontractility and markedly attenuates endothelium-dependent relaxation to acetylcholine
    Hans H Dietrich
    Washington Univ School of Medicine, Dept of Neurosurgery, 660 South Euclid Ave, Campus Box 8057, St Louis, MO 63110, USA
    Am J Physiol Heart Circ Physiol 298:H2208-20. 2010
    ....
  17. ncbi Microfluidics-based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi-dimensional mass spectrometry: development of an automated high-throughput platform f
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Rapid Commun Mass Spectrom 22:2115-24. 2008
    ....
  18. ncbi Shotgun lipidomics identifies cardiolipin depletion in diabetic myocardium linking altered substrate utilization with mitochondrial dysfunction
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 44:16684-94. 2005
    ....
  19. ncbi Factors influencing the electrospray intrasource separation and selective ionization of glycerophospholipids
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Am Soc Mass Spectrom 17:264-74. 2006
    ....
  20. ncbi Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of cellular glycerophospholipids enabled by multiplexed solvent dependent analyte-matrix interactions
    Gang Sun
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Chem 80:7576-85. 2008
    ....
  21. ncbi Nuclear receptors PPARbeta/delta and PPARalpha direct distinct metabolic regulatory programs in the mouse heart
    Eileen M Burkart
    Center for Cardiovascular Research, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Clin Invest 117:3930-9. 2007
    ....
  22. ncbi Alterations in myocardial cardiolipin content and composition occur at the very earliest stages of diabetes: a shotgun lipidomics study
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 46:6417-28. 2007
    ....
  23. ncbi CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine
    Fatiha Nassir
    Department of Medicine, Division of Nutritional Science, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:19493-501. 2007
    ..The findings support the role of CD36 in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, whereas CD36-independent mechanisms predominate in distal segments...
  24. ncbi Epidermal growth factor receptors are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: a shotgun lipidomics study
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26796-804. 2005
    ....
  25. ncbi Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Anal Biochem 330:317-31. 2004
    ..This new integrated platform provides a robust foundation for the automated analysis of multidimensional mass spectra to advance the level of analytical scripting in lipidomics to a new plateau...
  26. ncbi Shotgun lipidomics: multidimensional MS analysis of cellular lipidomes
    Xianlin Han
    Washington University School of Medicine, Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, St Louis, Missouri 63110, USA
    Expert Rev Proteomics 2:253-64. 2005
    ..Thus, understanding the biochemical mechanisms underlying lipid-mediated disease states will be greatly facilitated by the power of shotgun lipidomics...
  27. ncbi Shotgun lipidomics of phosphoethanolamine-containing lipids in biological samples after one-step in situ derivatization
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 46:1548-60. 2005
    ....
  28. ncbi Lipid rafts are enriched in arachidonic acid and plasmenylethanolamine and their composition is independent of caveolin-1 expression: a quantitative electrospray ionization/mass spectrometric analysis
    Linda J Pike
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, St Louis, Missouri 63110, USA
    Biochemistry 41:2075-88. 2002
    ..These data provide insight into the structure of lipid rafts and identify potential new roles for these domains in signal transduction...
  29. ncbi A critical role for PPARalpha-mediated lipotoxicity in the pathogenesis of diabetic cardiomyopathy: modulation by dietary fat content
    Brian N Finck
    Department of Medicine, Center for Cardiovascular Research, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 100:1226-31. 2003
    ..These results link dysregulation of the PPARalpha gene regulatory pathway to cardiac dysfunction in the diabetic and provide a rationale for serum lipid-lowering strategies in the treatment of diabetic cardiomyopathy...
  30. ncbi Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics
    Xianlin Han
    Division of Bioorganic Chemistry, Washington University School of Medicine, St. Louis, MO 63110, USA
    J Lipid Res 44:1071-9. 2003
    ..We believe that ESI/MS-facilitated lipidomics has now opened a critical door that will greatly increase our understanding of human disease...
  31. ncbi Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples
    Xianlin Han
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mass Spectrom Rev 24:367-412. 2005
    ....
  32. ncbi Sequential ordered fatty acid alpha oxidation and Delta9 desaturation are major determinants of lipid storage and utilization in differentiating adipocytes
    Xiong Su
    Department of Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Biochemistry 43:5033-44. 2004
    ..Collectively, these results underscore the role of peroxisomal fatty acid processing as an important determinant of the metabolic fate of fatty acids in the differentiating adipocyte...
  33. ncbi Genetic ablation of calcium-independent phospholipase A2gamma leads to alterations in mitochondrial lipid metabolism and function resulting in a deficient mitochondrial bioenergetic phenotype
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:34611-22. 2007
    ..We conclude that iPLA(2)gamma is essential for maintaining efficient bioenergetic mitochondrial function through tailoring mitochondrial membrane lipid metabolism and composition...
  34. ncbi Lipidomics in diabetes and the metabolic syndrome
    Richard W Gross
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Methods Enzymol 433:73-90. 2007
    ..Through the use and practice of shotgun lipidomics, new insights into the cardiovascular pathobiology manifest in diabetes and the metabolic syndrome can be accrued...
  35. ncbi Purification of recombinant human cPLA2 gamma and identification of C-terminal farnesylation, proteolytic processing, and carboxymethylation by MALDI-TOF-TOF analysis
    Christopher M Jenkins
    Division of Bioorganic Chemistry, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 42:11798-807. 2003
    ....
  36. ncbi Calcium-independent phospholipases in the heart: mediators of cellular signaling, bioenergetics, and ischemia-induced electrophysiologic dysfunction
    Ari Cedars
    Division of Cardiology, Department of Medicine, Washington University School of Medicine, Washington University, St Louis, MO 63110, USA
    J Cardiovasc Pharmacol 53:277-89. 2009
    ..This review will provide a brief overview of myocardial iPLA2s and their significance in cardiac pathology and physiology...
  37. ncbi Identification of hepatic peroxisomal phospholipase A(2) and characterization of arachidonic acid-containing choline glycerophospholipids in hepatic peroxisomes
    Jingyue Yang
    Department of Chemistry, Washington University, Campus Box 1134, One Brookings Drive, St Louis, MO 63130, USA
    FEBS Lett 546:247-50. 2003
    ....
  38. ncbi Lipin 2 is a liver-enriched phosphatidate phosphohydrolase enzyme that is dynamically regulated by fasting and obesity in mice
    Matthew C Gropler
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:6763-72. 2009
    ..Collectively, these data suggest that lipin 2 plays an important role as a hepatic PAP-1 enzyme...
  39. ncbi Group VIA phospholipase A2 forms a signaling complex with the calcium/calmodulin-dependent protein kinase IIbeta expressed in pancreatic islet beta-cells
    Zhepeng Wang
    Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:6840-9. 2005
    ....
  40. ncbi Systematic analysis of choline-containing phospholipids using multi-dimensional mass spectrometry-based shotgun lipidomics
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, Box 8020, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2924-36. 2009
    ....
  41. ncbi Accumulation of long-chain acylcarnitine and 3-hydroxy acylcarnitine molecular species in diabetic myocardium: identification of alterations in mitochondrial fatty acid processing in diabetic myocardium by shotgun lipidomics
    Xiong Su
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Biochemistry 44:5234-45. 2005
    ....
  42. ncbi Identification of calcium-independent phospholipase A2 (iPLA2) beta, and not iPLA2gamma, as the mediator of arginine vasopressin-induced arachidonic acid release in A-10 smooth muscle cells. Enantioselective mechanism-based discrimination of mammalian iPL
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 277:32807-14. 2002
    ....
  43. ncbi Shotgun lipidomics reveals the temporally dependent, highly diversified cardiolipin profile in the mammalian brain: temporally coordinated postnatal diversification of cardiolipin molecular species with neuronal remodeling
    Hua Cheng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 47:5869-80. 2008
    ..Collectively, these results provide a new perspective on the complexity of CL in neuronal signaling, mitochondrial bioenergetics, and apoptosis...
  44. ncbi Smooth muscle cell arachidonic acid release, migration, and proliferation are markedly attenuated in mice null for calcium-independent phospholipase A2beta
    Sung Ho Moon
    Division of Bioorganic Chemistry and Molecular Pharmacology, Endocrinology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:33975-87. 2008
    ..We conclude that iPLA(2)beta is an important mediator of AA release and prostaglandin E(2) production in SMCs, modulating vascular tone, cellular signaling, proliferation, and migration...
  45. ncbi The highly selective production of 2-arachidonoyl lysophosphatidylcholine catalyzed by purified calcium-independent phospholipase A2gamma: identification of a novel enzymatic mediator for the generation of a key branch point intermediate in eicosanoid sig
    Wei Yan
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:26669-79. 2005
    ....
  46. ncbi Shotgun lipidomics identifies a paired rule for the presence of isomeric ether phospholipid molecular species
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 2:e1368. 2007
    ..g., heart and the brain) that play important roles in cellular function. Alterations of ether phospholipid molecular species contents are associated with a number of genetic disorders and human diseases...
  47. ncbi Small interfering RNA knockdown of calcium-independent phospholipases A2 beta or gamma inhibits the hormone-induced differentiation of 3T3-L1 preadipocytes
    Xiong Su
    Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:21740-8. 2004
    ....
  48. ncbi G-protein signaling participates in the development of diabetic cardiomyopathy
    Ian S Harris
    Center for Cardiovascular Research, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Diabetes 53:3082-90. 2004
    ..These results establish that Gq-mediated signaling plays a critical role in the pathogenesis of diabetic cardiomyopathy...
  49. ncbi Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics
    Michael A Kiebish
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 51:2153-70. 2010
    ..We believe this study provides a foundation to investigate cardiolipin metabolism and bioenergetic homeostasis in normal and disease states...
  50. ncbi Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix
    Hua Cheng
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis MO 63110, USA
    J Lipid Res 51:1599-609. 2010
    ....
  51. ncbi Facile identification and quantitation of protein phosphorylation via beta-elimination and Michael addition with natural abundance and stable isotope labeled thiocholine
    Meng Chen
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Chem 82:163-71. 2010
    ....
  52. ncbi Genetic ablation of calcium-independent phospholipase A2{gamma} leads to alterations in hippocampal cardiolipin content and molecular species distribution, mitochondrial degeneration, autophagy, and cognitive dysfunction
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:35632-44. 2009
    ....
  53. ncbi Automated lipid identification and quantification by multidimensional mass spectrometry-based shotgun lipidomics
    Kui Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Chem 81:4356-68. 2009
    ..Accordingly, the development of this program should greatly accelerate high-throughput analysis of lipids using MDMS-based shotgun lipidomics...
  54. ncbi Disrupted membrane homeostasis and accumulation of ubiquitinated proteins in a mouse model of infantile neuroaxonal dystrophy caused by PLA2G6 mutations
    Ibrahim Malik
    Department of Neurology, Washington University School of Medicine, St Louis, MO 63110, USA
    Am J Pathol 172:406-16. 2008
    ..iPLA(2)beta-KO mice will be useful for further studies of pathogenesis and experimental interventions in INAD and neurodegeneration with brain iron accumulation...
  55. ncbi Cardiac ischemia activates calcium-independent phospholipase A2beta, precipitating ventricular tachyarrhythmias in transgenic mice: rescue of the lethal electrophysiologic phenotype by mechanism-based inhibition
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology. Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Biol Chem 278:22231-6. 2003
    ....
  56. ncbi Eicosanoid signalling pathways in the heart
    Christopher M Jenkins
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8020, St Louis, MO 63110, USA
    Cardiovasc Res 82:240-9. 2009
    ....
  57. ncbi Shotgun metabolomics approach for the analysis of negatively charged water-soluble cellular metabolites from mouse heart tissue
    Gang Sun
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Anal Chem 79:6629-40. 2007
    ..Collectively, these results identify a multiplexed MALDI-TOF/TOF MS approach for analysis of negatively charged metabolites in mammalian tissues...
  58. ncbi Submicromolar concentrations of palmitoyl-CoA specifically thioesterify cysteine 244 in glyceraldehyde-3-phosphate dehydrogenase inhibiting enzyme activity: a novel mechanism potentially underlying fatty acid induced insulin resistance
    Jingyue Yang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    Biochemistry 44:11903-12. 2005
    ....
  59. ncbi Alkaline methanolysis of lipid extracts extends shotgun lipidomics analyses to the low-abundance regime of cellular sphingolipids
    Xuntian Jiang
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Anal Biochem 371:135-45. 2007
    ..Accordingly, through utilization of these facile enrichment techniques, direct penetrance into the sphingolipidomes has been greatly extended, facilitating new insights into their metabolism and signaling functions in biological systems...
  60. ncbi Complex transcriptional and translational regulation of iPLAgamma resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization
    David J Mancuso
    Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA
    Eur J Biochem 271:4709-24. 2004
    ..Because uncoupling protein function is regulated by free fatty acids in mitochondria, these results suggest that iPLA2gamma processing contributes to integrating respiration and thermogenesis in mitochondria...
  61. ncbi Lysosomal phospholipase A2 and phospholipidosis
    Miki Hiraoka
    Nephrology Division, Department of Internal Medicine, University of Michigan, Room 1560 MSRB II, 1150 West Medical Center Dr, Ann Arbor, MI 48103 0676, USA
    Mol Cell Biol 26:6139-48. 2006
    ..Thus, a deficiency of lysosomal phospholipase A2 results in foam cell formation, surfactant lipid accumulation, splenomegaly, and phospholipidosis in mice...
  62. ncbi Adipocyte lipases and defect of lipolysis in human obesity
    Dominique Langin
    Obesity Research Unit, Institut National de la Sante et de la Recherche Medicale, Université Paul Sabatier UPS U586, Louis Bugnard Institute, Toulouse University Hospitals, Toulouse, France
    Diabetes 54:3190-7. 2005
    ..Decreased catecholamine-induced lipolysis and low HSL expression constitute a possibly primary defect in obesity...

Research Grants24

  1. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 1992
    ..Taken together, the proposed research is a multidisciplinary approach focused on identifying the detailed molecular interactions which modulate signal transduction, membrane dynamics and the development of the hypertensive state...
  2. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 2005
    ..Collectively, these studies represent a targeted, multidisciplinary approach to elucidate the roles of intracellular phospholipases in the vascular biology of the hypertensive and atherosclerotic disease states. ..
  3. MEMBRANE MECHANISMS MEDIATING ALCOHOL INDUCED PATHOLOGY
    Richard Gross; Fiscal Year: 2001
    ..Collectively, the proposed research represents a multidisciplinary approach designed to dissect the biochemical mechanisms underlying these novel effects of ethanol and ethanol metabolites on hippocampal function. ..
  4. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 2007
    ....
  5. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard Gross; Fiscal Year: 2000
    ..Collectively, these studies constitute a multidisciplinary approach aimed at elucidating the molecular mechanisms through which calcium-independent phospholipase A2 modulates vascular contractility and responsiveness. ..
  6. LIPID MEDIATORS OF SIGNAL TRANSDUCTION IN SMOOTH MUSCLE
    Richard W Gross; Fiscal Year: 2010
    ....