Will L Goff
Affiliation: Washington State University
Goff W, Johnson W, Parish S, Barrington G, Tuo W, Valdez R. The age-related immunity in cattle to Babesia bovis infection involves the rapid induction of interleukin-12, interferon-gamma and inducible nitric oxide synthase mRNA expression in the spleen. Parasite Immunol. 2001;23:463-71 pubmed
Goff W, Johnson W, Tuo W, Valdez R, Parish S, Barrington G, et al
. Age-related innate immune response in calves to Babesia bovis involves IL-12 induction and IL-10 modulation. Ann N Y Acad Sci. 2002;969:164-8 pubmed
..Also in contrast with calves, there was no detectable antibody response before death in adults. A vigorous CD8+ T-cell expansion occurred in the spleens of both calves and adults. ..
Goff W, Storset A, Johnson W, Brown W. Bovine splenic NK cells synthesize IFN-gamma in response to IL-12-containing supernatants from Babesia bovis-exposed monocyte cultures. Parasite Immunol. 2006;28:221-8 pubmed
..Finally, innate IFN-gamma production was induced in splenic NK cells in the presence of supernatants from B. bovis merozoite-exposed monocytes in an IL-12 pathway-dependent manner. ..
Goff W, Molloy J, Johnson W, Suarez C, Pino I, Rhalem A, et al
. Validation of a competitive enzyme-linked immunosorbent assay for detection of antibodies against Babesia bovis. Clin Vaccine Immunol. 2006;13:1212-6 pubmed
..Analysis of variance of the resulting optical densities and a test of homogeneity indicated no significant difference among the laboratories. Overall, the cELISA appears to have the attributes necessary for international application. ..
Goff W, Johnson W, Parish S, Barrington G, Elsasser T, Davis W, et al
. IL-4 and IL-10 inhibition of IFN-gamma- and TNF-alpha-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites. Vet Immunol Immunopathol. 2002;84:237-51 pubmed
..However, while merozoites plus IFN-gamma induced TNF-alpha mRNA expression in MDM, NO was not produced. Both IL-4 and IL-10 inhibited expression of iNOS and production of NO in merozoite-stimulated monocytes...
Goff W, McElwain T, Suarez C, Johnson W, Brown W, Norimine J, et al
. Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle. Clin Diagn Lab Immunol. 2003;10:38-43 pubmed
..3% concordance). The assay appears to have the characteristics necessary for an epidemiologic and disease surveillance tool...
Goff W, Johnson W, Horn R, Barrington G, Knowles D. The innate immune response in calves to Boophilus microplus tick transmitted Babesia bovis involves type-1 cytokine induction and NK-like cells in the spleen. Parasite Immunol. 2003;25:185-8 pubmed
..The proliferating CD8+ population consisted primarily of NK-like cells, and the expansion occurred in parallel with an increase in IL-15 mRNA expression in the spleen...
Goff W, Johnson W, Molloy J, Jorgensen W, Waldron S, Figueroa J, et al
. Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle. Clin Vaccine Immunol. 2008;15:1316-21 pubmed publisher
..8, indicating a high degree of reliability. Overall, the cELISA appears to have the attributes necessary for international application...