Elliot L Elson

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc The role of mechanics in actin stress fiber kinetics
    E L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, United States Department of Mechanical Engineering and Materials Science, Washington University, St Louis, MO 63130, United States Electronic address
    Exp Cell Res 319:2490-500. 2013
  2. doi request reprint Brief introduction to fluorescence correlation spectroscopy
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri, USA
    Methods Enzymol 518:11-41. 2013
  3. doi request reprint 40 years of FCS: how it all began
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri, USA
    Methods Enzymol 518:1-10. 2013
  4. pmc Fluorescence correlation spectroscopy: past, present, future
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Washington University in St Louis, St Louis, Missouri, USA
    Biophys J 101:2855-70. 2011
  5. ncbi request reprint Quick tour of fluorescence correlation spectroscopy from its inception
    Elliot L Elson
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, Campus Box 8231, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    J Biomed Opt 9:857-64. 2004
  6. pmc Phase separation in biological membranes: integration of theory and experiment
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, and Department of Physics, Washington University, St Louis, Missouri 63110, USA
    Annu Rev Biophys 39:207-26. 2010
  7. pmc Cellular and matrix mechanics of bioartificial tissues during continuous cyclic stretch
    Jeremiah J Wille
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    Ann Biomed Eng 34:1678-90. 2006
  8. pmc Thin bio-artificial tissues in plane stress: the relationship between cell and tissue strain, and an improved constitutive model
    J Pablo Marquez
    Department of Mechanical Engineering, Washington University, St Louis, Missouri 63130, USA
    Biophys J 88:765-77. 2005
  9. pmc Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy
    Krishnananda Chattopadhyay
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biophys J 88:1413-22. 2005
  10. pmc Cellular and matrix contributions to tissue construct stiffness increase with cellular concentration
    J Pablo Marquez
    Department of Mechanical and Aerospace Engineering, Campus Box 1185, Washington University, St Louis, MO 63130, USA
    Ann Biomed Eng 34:1475-82. 2006

Collaborators

Detail Information

Publications34

  1. pmc The role of mechanics in actin stress fiber kinetics
    E L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, United States Department of Mechanical Engineering and Materials Science, Washington University, St Louis, MO 63130, United States Electronic address
    Exp Cell Res 319:2490-500. 2013
    ..After providing a critical review of existing models, we propose lines of experimentation that might be able to test the different principles underlying their kinetic laws. ..
  2. doi request reprint Brief introduction to fluorescence correlation spectroscopy
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri, USA
    Methods Enzymol 518:11-41. 2013
    ..FCS provides a window on mesoscopic systems and is closely related to fluorescence methods for studying single molecules...
  3. doi request reprint 40 years of FCS: how it all began
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri, USA
    Methods Enzymol 518:1-10. 2013
    ..FCS provides a window on a mesoscopic world in which molecular fluctuations are both detectable and (for biological cells) possibly functionally important. Moreover, FCS is a precursor to single molecule measurements...
  4. pmc Fluorescence correlation spectroscopy: past, present, future
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Washington University in St Louis, St Louis, Missouri, USA
    Biophys J 101:2855-70. 2011
    ..For example, a new and potentially interesting field for FCS studies could be the study of nonequilibrium steady states, especially in living cells...
  5. ncbi request reprint Quick tour of fluorescence correlation spectroscopy from its inception
    Elliot L Elson
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, Campus Box 8231, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    J Biomed Opt 9:857-64. 2004
    ..From its inception to the present, the contributions of Webb and his coworkers have had a central and defining role in the development and applications of FCS...
  6. pmc Phase separation in biological membranes: integration of theory and experiment
    Elliot L Elson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, and Department of Physics, Washington University, St Louis, Missouri 63110, USA
    Annu Rev Biophys 39:207-26. 2010
    ..This review summarizes linkages between theoretical and experimental studies of phase separation in lipid bilayer model membranes...
  7. pmc Cellular and matrix mechanics of bioartificial tissues during continuous cyclic stretch
    Jeremiah J Wille
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    Ann Biomed Eng 34:1678-90. 2006
    ..25 Hz. Cell stiffness decreased with stretch amplitude (5-25%) at 0.1 Hz. The trends in cell stiffness do not fit simple viscoelastic models previously proposed, and suggest possible strain-amplitude related changes during cyclic stretch...
  8. pmc Thin bio-artificial tissues in plane stress: the relationship between cell and tissue strain, and an improved constitutive model
    J Pablo Marquez
    Department of Mechanical Engineering, Washington University, St Louis, Missouri 63130, USA
    Biophys J 88:765-77. 2005
    ..We find that, without the modification in this article, the Zahalak model can underpredict cell stiffness by an order of magnitude...
  9. pmc Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy
    Krishnananda Chattopadhyay
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biophys J 88:1413-22. 2005
    ..Although FCS experiments indicate that the unfolded state at pH 2 is rather compact and native-like, the radius in the presence of guanidine hydrochloride falls well within the range expected for a random coil...
  10. pmc Cellular and matrix contributions to tissue construct stiffness increase with cellular concentration
    J Pablo Marquez
    Department of Mechanical and Aerospace Engineering, Campus Box 1185, Washington University, St Louis, MO 63130, USA
    Ann Biomed Eng 34:1475-82. 2006
    ..The amplitudes of these responses were nearly linear with the amplitude of the applied stretch. The active component of cellular forces rose dramatically for FPMs containing higher cell concentrations...
  11. pmc Reconstitution of the Frank-Starling mechanism in engineered heart tissues
    Clara F Asnes
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biophys J 91:1800-10. 2006
    ..The experimental results strongly suggest that regulation of twitch tension in EHTs is similar to that of natural myocardium...
  12. pmc Physically-induced cytoskeleton remodeling of cells in three-dimensional culture
    Sheng Lin Lee
    Department of Mechanical Engineering and Materials Science, Washington University, St Louis, MO, USA
    PLoS ONE 7:e45512. 2012
    ..These findings suggest mechanisms for global actin cytoskeleton remodeling in non-muscle cells and provide insight into cellular responses important in pathologies such as fibrosis and hypertension...
  13. pmc Stretch-activated force shedding, force recovery, and cytoskeletal remodeling in contractile fibroblasts
    Ali Nekouzadeh
    Cardiac Bioelectricity and Arrhythmia Center, Department of Biomedical Engineering, Washington University in St Louis, St Louis, MO, USA
    J Biomech 41:2964-71. 2008
    ..This complementary action demonstrates a programmed regulatory mechanism that protects cells from excessive stretch through choreographed active mechanical and biochemical healing responses...
  14. pmc The relationship between cell and tissue strain in three-dimensional bio-artificial tissues
    J Pablo Marquez
    Department of Mechanical Engineering, and Department of Biochemistry and Molecular Biophysics, Washington University, St Louis, Missouri 63130, USA
    Biophys J 88:778-89. 2005
    ....
  15. pmc Whole cell mechanics of contractile fibroblasts: relations between effective cellular and extracellular matrix moduli
    J Pablo Marquez
    Mechanical, Aerospace, and Structural Engineering, Washington University in St Louis School of Medicine, St Louis, MO 63130, USA
    Philos Trans A Math Phys Eng Sci 368:635-54. 2010
    ..With increasing cell concentration, the short-term and long-term tangent moduli of the reconstituted collagen ECM increased sharply from a baseline value, while those of the cells decreased monotonically...
  16. pmc The kinetics of conformational fluctuations in an unfolded protein measured by fluorescence methods
    Krishnananda Chattopadhyay
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 102:2385-9. 2005
    ..These measurements, which should be widely applicable to other systems, can provide important information about the still incompletely understood conformational properties of unfolded proteins and the mechanism of protein folding...
  17. pmc A simplified approach to quasi-linear viscoelastic modeling
    Ali Nekouzadeh
    Department of Mechanical and Aerospace Engineering, Washington University, St Louis, MO, USA
    J Biomech 40:3070-8. 2007
    ..The "adaptive QLV model" based on this new approach predicts collagen behavior comparably to or better than existing models, with much less computation...
  18. ncbi request reprint Interstitial collagenase is a Brownian ratchet driven by proteolysis of collagen
    Saveez Saffarian
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 306:108-11. 2004
    ..The biological implications of MMP-1 acting as a molecular ratchet tethered to the cell surface suggest new mechanisms for its role in tissue remodeling and cell-matrix interaction...
  19. ncbi request reprint On the application of strain factors for approximation of the contribution of anisotropic cells to the mechanics of a tissue construct
    J Pablo Marquez
    Department of Mechanical and Aerospace Engineering, Campus Box 1185, Washington University, St Louis, MO 63130, USA
    J Biomech 39:2145-51. 2006
    ..The results presented in this paper provide an explicit measure of the effects of cellular anisotropy, and a mechanism for calculating the contributions of these effects to overall tissue mechanics when these effects are important...
  20. ncbi request reprint What fluorescence correlation spectroscopy can tell us about unfolded proteins
    Carl Frieden
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Adv Protein Chem 62:91-109. 2002
  21. pmc Confidence intervals for concentration and brightness from fluorescence fluctuation measurements
    Kenneth M Pryse
    Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St Louis, Missouri, USA
    Biophys J 103:898-906. 2012
    ..Using the BCa method and additional simulated fluctuation data, we find that confidence intervals can be reduced dramatically for a specific non-Gaussian beam profile...
  22. ncbi request reprint Myosin-based contraction is not necessary for cardiac c-looping in the chick embryo
    Mathieu C Rémond
    Department of Biomedical Engineering, Washington University, Campus Box 1097, St Louis, MO 63130, USA
    Anat Embryol (Berl) 211:443-54. 2006
    ..None of these drugs significantly affected looping during the studied stages. These results suggest that active contraction is not required for normal c-looping of the embryonic chick heart between stages 10 and 12...
  23. ncbi request reprint One-dimensional viscoelastic behavior of fibroblast populated collagen matrices
    Jessica E Wagenseil
    Department of Cell Biology and Physiology, Washington University School of Medicine, 660 S Euclid, CB 8228, St Louis, MO 63110, USA
    J Biomech Eng 125:719-25. 2003
    ..The peak force, hysteresis and shape of the force-stretch curve are affected by the stretch rate, rest period, stretch amplitude and the number and magnitude of preconditioning cycles...
  24. pmc Statistical analysis of fluorescence correlation spectroscopy: the standard deviation and bias
    Saveez Saffarian
    Department of Physics, Washington University, St Louis, Missouri 63110, USA
    Biophys J 84:2030-42. 2003
    ..We demonstrate that the value of the bias can be calculated and added back as a first-order correction to the experimental correlation function...
  25. ncbi request reprint The biochemical response of the heart to hypertension and exercise
    Tetsuro Wakatsuki
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Washington University Medical Center, Campus Box 8231, 660 South Euclid Avenue, St Louis, MI 63110 1093, USA
    Trends Biochem Sci 29:609-17. 2004
    ..Despite this progress, the mechanisms that activate fibroblasts to cause fibrosis and those that differentiate between exercise and hypertension to produce physiological and pathological responses, respectively, remain to be established...
  26. ncbi request reprint Role of actin polymerization in bending of the early heart tube
    Kimberly S Latacha
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    Dev Dyn 233:1272-86. 2005
    ..Taken together, these data suggest that ongoing actin polymerization is required for the bending component of cardiac c-looping, and we speculate that polymerization-driven myocardial cell shape changes cause this deformation...
  27. ncbi request reprint Incremental mechanics of collagen gels: new experiments and a new viscoelastic model
    Kenneth M Pryse
    Department of Biochemistry and Molecular Biophysics, Washington University, St Louis, MO 63110 1093, USA
    Ann Biomed Eng 31:1287-96. 2003
    ..Some additional tests show features of the response of collagen gels to cycles of application and removal of loading...
  28. ncbi request reprint Cell orientation influences the biaxial mechanical properties of fibroblast populated collagen vessels
    Jessica E Wagenseil
    Department of Biomedical Engineering, Washington University, One Brookings Dr, St Louis, MO 63110, USA
    Ann Biomed Eng 32:720-31. 2004
    ..After Cyto D treatment, the FPCVs remained more extensible in the direction perpendicular to the cell orientation, even though a preferred collagen fiber orientation was not observed in the microscopy images...
  29. ncbi request reprint Reciprocal interactions between cells and extracellular matrix during remodeling of tissue constructs
    Tetsuro Wakatsuki
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Campus Box 8231, 660 South Euclid Avenue, St Louis, MO 63110 1093, USA
    Biophys Chem 100:593-605. 2003
    ....
  30. pmc Measurement of microsecond dynamic motion in the intestinal fatty acid binding protein by using fluorescence correlation spectroscopy
    Krishnananda Chattopadhyay
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 99:14171-6. 2002
    ..5. Steady state fluorescence and far-UV CD indicates that unfolding occurs at pH values below pH 3...
  31. ncbi request reprint Mechanics of cell spreading: role of myosin II
    Tetsuro Wakatsuki
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Sci 116:1617-25. 2003
    ..Cell indentation measurements show that a myosin-dependent viscoelastic force resists cell deformation...
  32. ncbi request reprint Epidermal growth factor induces acute matrix contraction and subsequent calpain-modulated relaxation
    Fred D Allen
    Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Wound Repair Regen 10:67-76. 2002
    ..Countervailing processes that down-regulate calpain activation can, accordingly, direct the transition of cell function from locomotion to matrix contraction...
  33. pmc Rho-kinase-mediated Ca2+-independent contraction in rat embryo fibroblasts
    Daniel A Emmert
    Department of Pathology, Saint Louis University School of Medicine, 1402 S Grand Blvd, St Louis, MO 63104, USA
    Am J Physiol Cell Physiol 286:C8-21. 2004
    ..These results suggest that Rho-kinase is sufficient to activate myosin II motor activity and contraction in REF-52 cells...
  34. pmc Fluorescence correlation spectroscopy with high-order and dual-color correlation to probe nonequilibrium steady states
    Hong Qian
    Department of Applied Mathematics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 101:2828-33. 2004
    ....