Susan Dutcher

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc Whole genome sequencing identifies a deletion in protein phosphatase 2A that affects its stability and localization in Chlamydomonas reinhardtii
    Huawen Lin
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Genet 9:e1003841. 2013
  2. pmc Identification of cilia genes that affect cell-cycle progression using whole-genome transcriptome analysis in Chlamydomonas reinhardtti
    Alison J Albee
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    G3 (Bethesda) 3:979-91. 2013
  3. pmc Katanin localization requires triplet microtubules in Chlamydomonas reinhardtii
    Jessica M Esparza
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 8:e53940. 2013
  4. pmc Synthesizing and salvaging NAD: lessons learned from Chlamydomonas reinhardtii
    Huawen Lin
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Genet 6:e1001105. 2010
  5. pmc Improving gene-finding in Chlamydomonas reinhardtii:GreenGenie2
    Alan L Kwan
    Department of Computer Science and Engineering, Washington University in Saint Louis, Saint Louis, MO, USA
    BMC Genomics 10:210. 2009
  6. ncbi request reprint Finding treasures in frozen cells: new centriole intermediates
    Susan K Dutcher
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioessays 29:630-4. 2007
  7. ncbi request reprint Chlamydomonas reinhardtii: biological rationale for genomics
    S K Dutcher
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, USA
    J Eukaryot Microbiol 47:340-9. 2000
  8. ncbi request reprint Elucidation of basal body and centriole functions in Chlamydomonas reinhardtii
    Susan K Dutcher
    Department of Genetics, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    Traffic 4:443-51. 2003
  9. ncbi request reprint The tubulin fraternity: alpha to eta
    S K Dutcher
    Department of Genetics, School of Medicine, Washington University, Campus Box 8232, 4566 Scott Avenue, St Louis, Missouri 63110, USA
    Curr Opin Cell Biol 13:49-54. 2001
  10. ncbi request reprint Long-lost relatives reappear: identification of new members of the tubulin superfamily
    Susan K Dutcher
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Opin Microbiol 6:634-40. 2003

Research Grants

  1. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan K Dutcher; Fiscal Year: 2010
  2. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 1999
  3. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2009
  4. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2007
  5. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2005
  6. International Conference on Cell and Molecular Biology of Chlamydomonas
    Susan Dutcher; Fiscal Year: 2006
  7. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2006
  8. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2005
  9. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2004
  10. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2003

Collaborators

Detail Information

Publications24

  1. pmc Whole genome sequencing identifies a deletion in protein phosphatase 2A that affects its stability and localization in Chlamydomonas reinhardtii
    Huawen Lin
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Genet 9:e1003841. 2013
    ..Overall, our results demonstrate that the terminal YFL tail of PP2A3 is important in the regulation on Chlamydomonas mating. ..
  2. pmc Identification of cilia genes that affect cell-cycle progression using whole-genome transcriptome analysis in Chlamydomonas reinhardtti
    Alison J Albee
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    G3 (Bethesda) 3:979-91. 2013
    ..These results demonstrate that whole-genome transcriptome analysis during ciliogenesis is a powerful tool to gain insight into the molecular mechanism by which centrosomes and cilia are assembled. ..
  3. pmc Katanin localization requires triplet microtubules in Chlamydomonas reinhardtii
    Jessica M Esparza
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 8:e53940. 2013
    ..We suggest that the triplet microtubules are likely to play a key role in katanin p80 recruitment to the basal body of Chlamydomonas rather than the transition fibers that are needed for IFT localization...
  4. pmc Synthesizing and salvaging NAD: lessons learned from Chlamydomonas reinhardtii
    Huawen Lin
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Genet 6:e1001105. 2010
    ..Our results suggest that Chlamydomonas is an excellent model system to study NAD+ metabolism and cell longevity...
  5. pmc Improving gene-finding in Chlamydomonas reinhardtii:GreenGenie2
    Alan L Kwan
    Department of Computer Science and Engineering, Washington University in Saint Louis, Saint Louis, MO, USA
    BMC Genomics 10:210. 2009
    ..To improve ab initio gene finding in Chlamydomonas, we assemble a new training set consisting of over 2,300 cDNAs by assembling over 167,000 Chlamydomonas EST entries in GenBank using the EST assembly tool PASA...
  6. ncbi request reprint Finding treasures in frozen cells: new centriole intermediates
    Susan K Dutcher
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioessays 29:630-4. 2007
    ..Surprising, the first intermediate is a hollow tube of 60 nm that increases in diameter and then elongates before acquiring microtubules. Similar structures have not been observed to date in other centrioles...
  7. ncbi request reprint Chlamydomonas reinhardtii: biological rationale for genomics
    S K Dutcher
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri, USA
    J Eukaryot Microbiol 47:340-9. 2000
    ..Thus, a genomic approach in Chlamydomonas will provide another important avenue for the understanding of important biological processes...
  8. ncbi request reprint Elucidation of basal body and centriole functions in Chlamydomonas reinhardtii
    Susan K Dutcher
    Department of Genetics, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    Traffic 4:443-51. 2003
    ..Genes for two new members of the tubulin superfamily are needed for basal body/centriole duplication. In addition, other genes that play roles in the duplication and segregation of basal bodies are discussed...
  9. ncbi request reprint The tubulin fraternity: alpha to eta
    S K Dutcher
    Department of Genetics, School of Medicine, Washington University, Campus Box 8232, 4566 Scott Avenue, St Louis, Missouri 63110, USA
    Curr Opin Cell Biol 13:49-54. 2001
    ..Recent genetic analyses and database searches have added four new members of the tubulin superfamily, which now includes alpha-, beta-, gamma-, delta-, epsilon-, zeta-, and eta-tubulin...
  10. ncbi request reprint Long-lost relatives reappear: identification of new members of the tubulin superfamily
    Susan K Dutcher
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Opin Microbiol 6:634-40. 2003
    ..Phylogenetic analysis suggests that it is most closely related to delta-tubulin, which suggests that delta- and eta-tubulin could have overlapping functions...
  11. pmc Epsilon-tubulin is an essential component of the centriole
    Susan K Dutcher
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Biol Cell 13:3859-69. 2002
    ..Polyclonal antibodies to epsilon-tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, epsilon-tubulin is encoded by the BLD2 allele and epsilon-tubulin plays a role in basal body/centriole morphogenesis...
  12. ncbi request reprint Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene
    Jin Billy Li
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell 117:541-52. 2004
    ..We show that this novel protein localizes to basal bodies in mouse and C. elegans, is under the regulatory control of daf-19, and is necessary for the generation of both cilia and flagella...
  13. ncbi request reprint Two flagellar genes, AGG2 and AGG3, mediate orientation to light in Chlamydomonas
    Carlo Iomini
    Department of Molecular, Cellular, and Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, Box 1007, New York, New York 10029, USA
    Curr Biol 16:1147-53. 2006
    ..We propose that the localization of Agg2p and Agg3p to the proximal region of the flagella may be important for interpreting light signals...
  14. ncbi request reprint Procom: a web-based tool to compare multiple eukaryotic proteomes
    Jin Billy Li
    Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
    Bioinformatics 21:1693-4. 2005
    ..To facilitate these comparisons, a web-based server, Procom, was developed to identify the subset of genes that may be needed for a trait...
  15. pmc Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii
    Carlo Iomini
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Genetics 183:885-96. 2009
    ..Since these tests show different levels of phenotypic sensitivity, we propose they illustrate different gradations of gene interaction between complex A proteins themselves and with a complex B protein (IFT172)...
  16. ncbi request reprint Analysis of Chlamydomonas reinhardtii genome structure using large-scale sequencing of regions on linkage groups I and III
    Jin Billy Li
    Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Eukaryot Microbiol 50:145-55. 2003
    ..Finally recombination differs between the two sequenced contigs. The 350-Kb of the Linkage group III contig is devoid of recombination, while the Linkage group I contig is 30 map units long over 33-kb...
  17. pmc Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins
    Amber K Bowers
    Department of Genetics, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Genetics 164:1345-53. 2003
    ..Genomic DNA for RPL22 from wild type and three mutant ery2 alleles was amplified and sequenced and revealed three different point mutations. Two alleles have premature stop codons and one allele changes a splice site acceptor site...
  18. pmc Three-dimensional organization of basal bodies from wild-type and delta-tubulin deletion strains of Chlamydomonas reinhardtii
    Eileen T O'Toole
    Boulder Laboratory for 3 D Fine Structure, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Mol Biol Cell 14:2999-3012. 2003
    ..These data suggest that the presence of specific tubulin isoforms in Chlamydomonas directly affects the assembly and function of both basal bodies and basal body-associated structures...
  19. ncbi request reprint The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants
    Stefan A Rensing
    Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestrasse 1, D 79104 Freiburg, Germany
    Science 319:64-9. 2008
    ..The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics...
  20. ncbi request reprint A revised nomenclature for the human and rodent alpha-tubulin gene family
    Varsha K Khodiyar
    HUGO Gene Nomenclature Committee, Department of Biology, University College London, Wolfson House, 4 Stephenson Way, London NW1 2HE, UK
    Genomics 90:285-9. 2007
    ..This revised nomenclature provides a unified language for the discussion of these genes in mammalian species; it has been approved by the gene nomenclature committees of the three species and is supported by researchers in the field...
  21. ncbi request reprint Mutations in alpha-tubulin promote basal body maturation and flagellar assembly in the absence of delta-tubulin
    Sylvia Fromherz
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309 0347, USA
    J Cell Sci 117:303-14. 2004
    ..We suggest that the mutant alpha-tubulin promotes the early maturation of the basal body in the absence of delta-tubulin, perhaps through interactions with other partners, and this allows assembly of the flagella...
  22. ncbi request reprint Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography
    Eileen T O'Toole
    Boulder Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Methods Cell Biol 79:125-43. 2007
  23. pmc Mutant kinesin-2 motor subunits increase chromosome loss
    Mark S Miller
    Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844 3052, USA
    Mol Biol Cell 16:3810-20. 2005
    ..The analysis of the mutants and the revertants demonstrates the importance of a pocket in the amino terminus of these motor subunits for both motor activity and for a novel, dominant effect on the fidelity of chromosome segregation...

Research Grants15

  1. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan K Dutcher; Fiscal Year: 2010
    ..We propose to use Chlamydomonas to study duplication and function of centrioles. ..
  2. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 1999
    ..Gal4p activation of downstream reporter sequences will be used to identify cDNA clones which encode gene products which physically interact with centrin. ..
  3. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2009
    ..We will mine using "comparative genomics" to identify additional genes that are needed for duplication, maturation, and migration of the centrioles/basal bodies. ..
  4. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2007
    ..We will mine using "comparative genomics" to identify additional genes that are needed for duplication, maturation, and migration of the centrioles/basal bodies. ..
  5. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2005
    ..We will mine using "comparative genomics" to identify additional genes that are needed for duplication, maturation, and migration of the centrioles/basal bodies. ..
  6. International Conference on Cell and Molecular Biology of Chlamydomonas
    Susan Dutcher; Fiscal Year: 2006
    ..This provides a chance for workers in Chlamydomonas to gain information on new techniques and ideas. ..
  7. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2006
    ..We will mine using "comparative genomics" to identify additional genes that are needed for duplication, maturation, and migration of the centrioles/basal bodies. ..
  8. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2005
    ..We will mine using "comparative genomics" to identify additional genes that are needed for duplication, maturation, and migration of the centrioles/basal bodies. ..
  9. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2004
    ..We will mine using "comparative genomics" to identify additional genes that are needed for duplication, maturation, and migration of the centrioles/basal bodies. ..
  10. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2003
    ..abstract_text> ..
  11. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2002
    ..abstract_text> ..
  12. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2001
    ..abstract_text> ..
  13. GENETIC ANALYSIS OF BASAL BODY FUNCTION
    Susan Dutcher; Fiscal Year: 2000
    ..abstract_text> ..