Research Topics
Species | Susan DutcherSummaryAffiliation: Washington University School of Medicine Country: USA Publications
Research Grants
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Detail Information
Publications
Improving gene-finding in Chlamydomonas reinhardtii:GreenGenie2Alan L Kwan
Department of Computer Science and Engineering, Washington University in Saint Louis, Saint Louis, MO, USA
BMC Genomics 10:210. 2009..To improve ab initio gene finding in Chlamydomonas, we assemble a new training set consisting of over 2,300 cDNAs by assembling over 167,000 Chlamydomonas EST entries in GenBank using the EST assembly tool PASA...
Chlamydomonas reinhardtii: biological rationale for genomicsS K Dutcher
Department of Genetics, Washington University School of Medicine, St Louis, Missouri, USA
J Eukaryot Microbiol 47:340-9. 2000..Thus, a genomic approach in Chlamydomonas will provide another important avenue for the understanding of important biological processes...- The tubulin fraternity: alpha to etaS K Dutcher
Department of Genetics, School of Medicine, Washington University, Campus Box 8232, 4566 Scott Avenue, St Louis, Missouri 63110, USA
Curr Opin Cell Biol 13:49-54. 2001..Recent genetic analyses and database searches have added four new members of the tubulin superfamily, which now includes alpha-, beta-, gamma-, delta-, epsilon-, zeta-, and eta-tubulin... - Elucidation of basal body and centriole functions in Chlamydomonas reinhardtiiSusan K Dutcher
Department of Genetics, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
Traffic 4:443-51. 2003..Genes for two new members of the tubulin superfamily are needed for basal body/centriole duplication. In addition, other genes that play roles in the duplication and segregation of basal bodies are discussed... - Finding treasures in frozen cells: new centriole intermediatesSusan K Dutcher
Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
Bioessays 29:630-4. 2007..Surprising, the first intermediate is a hollow tube of 60 nm that increases in diameter and then elongates before acquiring microtubules. Similar structures have not been observed to date in other centrioles... - Long-lost relatives reappear: identification of new members of the tubulin superfamilySusan K Dutcher
Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
Curr Opin Microbiol 6:634-40. 2003..Phylogenetic analysis suggests that it is most closely related to delta-tubulin, which suggests that delta- and eta-tubulin could have overlapping functions... - Epsilon-tubulin is an essential component of the centrioleSusan K Dutcher
Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
Mol Biol Cell 13:3859-69. 2002..Polyclonal antibodies to epsilon-tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, epsilon-tubulin is encoded by the BLD2 allele and epsilon-tubulin plays a role in basal body/centriole morphogenesis... - Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease geneJin Billy Li
Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA
Cell 117:541-52. 2004..We show that this novel protein localizes to basal bodies in mouse and C. elegans, is under the regulatory control of daf-19, and is necessary for the generation of both cilia and flagella... - Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtiiCarlo Iomini
Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
Genetics 183:885-96. 2009..Since these tests show different levels of phenotypic sensitivity, we propose they illustrate different gradations of gene interaction between complex A proteins themselves and with a complex B protein (IFT172)... - Procom: a web-based tool to compare multiple eukaryotic proteomesJin Billy Li
Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA
Bioinformatics 21:1693-4. 2005..AVAILABILITY: The Procom program is freely available with documentation and examples at http://ural.wustl.edu/~billy/Procom/ CONTACT: billy@ural.wustl.edu... - Two flagellar genes, AGG2 and AGG3, mediate orientation to light in ChlamydomonasCarlo Iomini
Department of Molecular, Cellular, and Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, Box 1007, New York, New York 10029, USA
Curr Biol 16:1147-53. 2006..We propose that the localization of Agg2p and Agg3p to the proximal region of the flagella may be important for interpreting light signals... - Analysis of Chlamydomonas reinhardtii genome structure using large-scale sequencing of regions on linkage groups I and IIIJin Billy Li
Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Eukaryot Microbiol 50:145-55. 2003..Finally recombination differs between the two sequenced contigs. The 350-Kb of the Linkage group III contig is devoid of recombination, while the Linkage group I contig is 30 map units long over 33-kb... - Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteinsAmber K Bowers
Department of Genetics, Washington University School of Medicine, 660 S. Euclid Avenue, St Louis, MO 63110, USA
Genetics 164:1345-53. 2003..Genomic DNA for RPL22 from wild type and three mutant ery2 alleles was amplified and sequenced and revealed three different point mutations. Two alleles have premature stop codons and one allele changes a splice site acceptor site... - The Physcomitrella genome reveals evolutionary insights into the conquest of land by plantsStefan A Rensing
Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestrasse 1, D 79104 Freiburg, Germany
Science 319:64-9. 2008..The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics... - Three-dimensional organization of basal bodies from wild-type and delta-tubulin deletion strains of Chlamydomonas reinhardtiiEileen T O'Toole
Boulder Laboratory for 3 D Fine Structure, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Mol Biol Cell 14:2999-3012. 2003..These data suggest that the presence of specific tubulin isoforms in Chlamydomonas directly affects the assembly and function of both basal bodies and basal body-associated structures... - A revised nomenclature for the human and rodent alpha-tubulin gene familyVarsha K Khodiyar
HUGO Gene Nomenclature Committee, Department of Biology, University College London, Wolfson House, 4 Stephenson Way, London NW1 2HE, UK
Genomics 90:285-9. 2007..This revised nomenclature provides a unified language for the discussion of these genes in mammalian species; it has been approved by the gene nomenclature committees of the three species and is supported by researchers in the field... - Mutations in alpha-tubulin promote basal body maturation and flagellar assembly in the absence of delta-tubulinSylvia Fromherz
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309 0347, USA
J Cell Sci 117:303-14. 2004..We suggest that the mutant alpha-tubulin promotes the early maturation of the basal body in the absence of delta-tubulin, perhaps through interactions with other partners, and this allows assembly of the flagella... - Mutant kinesin-2 motor subunits increase chromosome lossMark S Miller
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844-3052, USA
Mol Biol Cell 16:3810-20. 2005..The analysis of the mutants and the revertants demonstrates the importance of a pocket in the amino terminus of these motor subunits for both motor activity and for a novel, dominant effect on the fidelity of chromosome segregation... - Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomographyThomas H Giddings
Boulder Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Methods Cell Biol 79:125-43. 2007
Research Grants
- GENETIC ANALYSIS OF BASAL BODY FUNCTIONSusan K Dutcher; Fiscal Year: 2010..We propose to use Chlamydomonas to study duplication and function of centrioles. ..
- GENETIC ANALYSIS OF BASAL BODY FUNCTIONSusan Dutcher; Fiscal Year: 1999..Gal4p activation of downstream reporter sequences will be used to identify cDNA clones which encode gene products which physically interact with centrin. ..
- GENETIC ANALYSIS OF BASAL BODY FUNCTIONSusan Dutcher; Fiscal Year: 2009..We will mine using "comparative genomics" to identify additional genes that are needed for duplication, maturation, and migration of the centrioles/basal bodies. ..
- International Conference on Cell and Molecular Biology of ChlamydomonasSusan Dutcher; Fiscal Year: 2006..This provides a chance for workers in Chlamydomonas to gain information on new techniques and ideas. ..
- GENETIC ANALYSIS OF BASAL BODY FUNCTIONSusan Dutcher; Fiscal Year: 2003..abstract_text> ..