Research Topics
| Wen Ji DongSummaryAffiliation: Washington State University Country: USA Publications
Research Grants
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Detail Information
Publications
Effects of PKA phosphorylation of cardiac troponin I and strong crossbridge on conformational transitions of the N-domain of cardiac troponin C in regulated thin filamentsWen Ji Dong
The School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington 99164, USA
Biochemistry 46:9752-61. 2007..These results provide an insight into the modulation mechanism of strong crossbridge and cTnI phosphorylation in cardiac thin filament activation/relaxation processes...- Structural transition of the inhibitory region of troponin I within the regulated cardiac thin filamentWen Ji Dong
School of Chemical Engineering and Bioengineering and Department of Veterinary and Comparative Anatomy Pharmacology and Physiology, Washington State University, Pullman, WA 99164, USA
Arch Biochem Biophys 456:135-42. 2006..These results support the idea of a feedback mechanism by strong cross-bridge interaction with actin and provide insights on the molecular basis for the fine tuning of cardiac function by beta-adrenergic stimulation... 
Structural kinetics of cardiac troponin C mutants linked to familial hypertrophic and dilated cardiomyopathy in troponin complexesWen Ji Dong
School of Chemical Engineering and Bioengineering, Washington State University, Pullman 99164, USA
J Biol Chem 283:3424-32. 2008..Both mutations appear to be antagonistic toward phosphorylation signaling between cTnI and cTnC...- Förster resonance energy transfer structural kinetic studies of cardiac thin filament deactivationJun Xing
School of Chemical Engineering and Bioengineering, Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University, Pullman, WA 99164, USA
J Biol Chem 284:16432-41. 2009..The other step involves switching the inhibitory region of cTnI from interacting with cTnC to interacting with actin. The latter processes may play a key role in regulating cross-bridge kinetics... - Preconcentration and detection of the phosphorylated forms of cardiac troponin I in a cascade microchip by cationic isotachophoresisDanny Bottenus
Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164 2710, USA
Lab Chip 11:3793-801. 2011..To our knowledge, this is the first attempt at using ITP in a cascade microchip to quantify cTnI in human serum and detect different phosphorylated forms... - Structural and kinetic effects of PAK3 phosphorylation mimic of cTnI(S151E) on the cTnC-cTnI interaction in the cardiac thin filamentYexin Ouyang
Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA
J Mol Biol 400:1036-45. 2010..Our results provide novel information on how cardiac thin filament regulation is modulated by PAK3 phosphorylation of cTnI... - Structural dynamics of C-domain of cardiac troponin I protein in reconstituted thin filamentZhiqun Zhou
Department of Veterinary and Comparative Anatomy Pharmacology and Physiology, Washington State University, Pullman, Washington 99164, USA
J Biol Chem 287:7661-74. 2012..In contrast, the IR responds to Ca(2+) signals with slow structural dynamics and transition kinetics. The collective findings suggested a fourth state of activation... 
Structural and kinetic effects of hypertrophic cardiomyopathy related mutations R146G/Q and R163W on the regulatory switching activity of rat cardiac troponin IZhiqun Zhou
Department of Veterinary and Comparative Anatomy Pharmacology and Physiology, Washington State University, Pullman, WA 99164, USA
Arch Biochem Biophys 535:56-67. 2013..In situ rat myocardial fiber studies also revealed that FRET distance changes indicating mutation specific disruption of the cTnIIR-actin interaction were consistent with increased passive tension...- Enhanced fluorescence anisotropy assay for human cardiac troponin I and T detectionYanling Qiao
Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA
J Fluoresc 21:2101-10. 2011..Homogenous nature, rapid response time, and easy implementation of our assay design make it a useful tool for disease biomarker and protein sensing... - 10,000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresisDanny Bottenus
Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, P O Box 642710, Pullman, WA 99164 2710, USA
Lab Chip 11:890-8. 2011..This experimental approach could be coupled to an immunoassay based technique and has the potential to lower limits of detection, increase sensitivity, and quantify different isolated cTnI phosphorylation states... - Enzymatic amplification of DNA/RNA hybrid molecular beacon signaling in nucleic acid detectionThomas Jacroux
Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA
Anal Biochem 432:106-14. 2013..Hence, the R/D-MB-RNase H scheme can be applied to a broad range of nucleic acid amplification methods... - Ca2+-induced conformational transition in the inhibitory and regulatory regions of cardiac troponin IWen-Ji Dong
Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham 35294-2041, USA
J Biol Chem 278:8686-92. 2003..We have used the data to construct a structural model of the cTnI inhibitory and regulatory regions in the Mg(2+)- and Ca(2+)-saturated states... - The calcium-saturated cTnI/cTnC complex: structure of the inhibitory region of cTnIChristopher Sheldahl
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Biophys J 84:1057-64. 2003..Although it is not possible to eliminate any of these models at this time, future studies with additional experimental restraints will yield insights on the mechanisms of calcium regulation in cardiac muscle... - Energy transduction optical sensor in skeletal myosinThomas P Burghardt
Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street Southwest, Rochester, Minnesota 55905, USA
Biochemistry 42:5877-84. 2003..Rationalizing the discrepancy invites further investigation of S1 dynamical structure local to the AST during transduction... - Can Förster resonance energy transfer measurements uniquely position troponin residues on the actin filament? A case study in multiple-acceptor FRETJohn M Robinson
Department of Biochemistry and Molecular Genetics University of Alabama at Birmingham, 35294 2041, USA
J Mol Biol 329:371-80. 2003..3A, laterally along the actin surface toward actin subdomain 3 by 22.0A, and azimuthally toward the actin inner domain by 13.2A. This information is used to construct a low-resolution structural model of thin filament activation... - Small-angle neutron scattering with contrast variation reveals spatial relationships between the three subunits in the ternary cardiac troponin complex and the effects of troponin I phosphorylationWilliam T Heller
Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
Biochemistry 42:7790-800. 2003..The rodlike cTnI bends sharply at the end interacting with the cTnC/cTnT(198-298) component, which reorients so as to maintain its contacts with cTnI while undergoing only a relatively small change in shape... - Kinetics of conformational transitions in cardiac troponin induced by Ca2+ dissociation determined by Förster resonance energy transferWen Ji Dong
Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, AL 35294 0005, USA
J Biol Chem 278:42394-402. 2003..The kinetic results define the magnitudes of structural changes relevant in Ca2+ switching between activation and deactivation of cardiac muscle contraction... - Effects of protein kinase C dependent phosphorylation and a familial hypertrophic cardiomyopathy-related mutation of cardiac troponin I on structural transition of troponin C and myofilament activationTomoyoshi Kobayashi
Department of Physiology and Biophysics, Center for Cardiovascular Research, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612 7342, USA
Biochemistry 43:5996-6004. 2004..Our data also support the hypothesis that pathological effects of phosphorylation are influenced by mutations in cTnI... - Switching of troponin I: Ca(2+) and myosin-induced activation of heart muscleJohn M Robinson
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294 2041, USA
J Mol Biol 340:295-305. 2004..Strong cross-bridges eliminate cooperativity and re-sensitize the CRS to Ca(2+). We propose a kinetic scheme and a structural model to account for these findings... - Förster resonance energy transfer measurements are consistent with a helical bundle model for lipid-free apolipoprotein A-IChristie G Brouillette
Center for Biophysical Sciences and Engineering, Department of Vision Sciences, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Biochemistry 44:16413-25. 2005..The results are consistent with the presence of a single folded domain within residues 1-186. These results support the presence of a discrete globular bundle conformation for lipid-free apo A-I... - Site-specific transfer of an intact beta-globin gene cluster through a new targeting vectorHai Sheng Zhou
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China
Biochem Biophys Res Commun 356:32-7. 2007..The results proved that the human chromosome-derived vector was specifically targeted to the human genome and this could provide a novel platform for further gene therapy research...
Research Grants
- Kinetics of Cardiac Myofilament ActivationWen Ji Dong; Fiscal Year: 2009..Results of this study will enhance our understanding of molecular mechanisms of thin filament activation in response of Ca2+ and the role of protein phosphorylation in heart failure. ..
- Kinetics of Cardiac Myofilament ActivationWen Ji Dong; Fiscal Year: 2010..This application is related to research to improve depressed cardiac contractility under pathological conditions. ..