Michael Diamond

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi request reprint Characterization of a panel of monoclonal antibodies and mapping the epitopes of hepatitis C virus proteins
    O V Masalova
    Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, ul Gamaleya 16, Moscow, 123098 Russia
    Dokl Biochem Biophys 383:125-9. 2002
  2. pmc Non-structural protein-1 is required for West Nile virus replication complex formation and viral RNA synthesis
    Soonjeon Youn
    Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Virol J 10:339. 2013
  3. pmc Host restriction factor screening: let the virus do the work
    Michael S Diamond
    Departments of Medicine, Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA Electronic address
    Cell Host Microbe 14:229-31. 2013
  4. pmc The broad-spectrum antiviral functions of IFIT and IFITM proteins
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nat Rev Immunol 13:46-57. 2013
  5. pmc Immune responses to West Nile virus infection in the central nervous system
    Hyelim Cho
    Departments of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Viruses 4:3812-30. 2012
  6. pmc Cell-intrinsic innate immune control of West Nile virus infection
    Michael S Diamond
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Trends Immunol 33:522-30. 2012
  7. pmc Recent progress in West Nile virus diagnosis and vaccination
    Marina De Filette
    Laboratory of Gene Therapy, Faculty of Veterinary Sciences, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium
    Vet Res 43:16. 2012
  8. pmc A tetravalent dengue nanoparticle stimulates antibody production in mice
    Elisângela F Silva
    Institute of Biomedical Science, Federal University of Alfenas, Minas Gerais, Brazil
    J Nanobiotechnology 10:13. 2012
  9. pmc Enhancement of anti-DIII antibodies by the C3d derivative P28 results in lower viral titers and augments protection in mice
    Matthew D Dunn
    Center for Vaccine Research, University of Pittsburgh, 9047 Biomedical Science Tower 3, Pittsburgh, PA 15261, USA
    Virol J 7:95. 2010
  10. pmc Actively replicating West Nile virus is resistant to cytoplasmic delivery of siRNA
    Brian J Geiss
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    Virol J 2:53. 2005

Research Grants

  1. Antibody Based Therapeuticcs for Chikungunya Virus
    Michael S Diamond; Fiscal Year: 2010
  2. INTERFERON IN MODULATING DENGUE VIRUS INFECTION
    Michael Diamond; Fiscal Year: 2002
  3. Antibody-Based Protection Against Dengue Virus
    Michael S Diamond; Fiscal Year: 2010
  4. Antibody-Based Protection Against Dengue Virus
    Michael Diamond; Fiscal Year: 2007
  5. Monoclonal Antibody Therapy for West Nile Virus
    Michael Diamond; Fiscal Year: 2007
  6. Development of Novel Antiviral Agents VS West Nile Virus
    Michael Diamond; Fiscal Year: 2006

Collaborators

Detail Information

Publications95

  1. ncbi request reprint Characterization of a panel of monoclonal antibodies and mapping the epitopes of hepatitis C virus proteins
    O V Masalova
    Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences, ul Gamaleya 16, Moscow, 123098 Russia
    Dokl Biochem Biophys 383:125-9. 2002
  2. pmc Non-structural protein-1 is required for West Nile virus replication complex formation and viral RNA synthesis
    Soonjeon Youn
    Department of Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Virol J 10:339. 2013
    ..How NS1 modulates infection remains uncertain given that the viral RNA replication complex localizes to the cytosolic face of the endoplasmic reticulum...
  3. pmc Host restriction factor screening: let the virus do the work
    Michael S Diamond
    Departments of Medicine, Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA Electronic address
    Cell Host Microbe 14:229-31. 2013
    ..2013) engineer a library of RNA viruses to express small interfering RNAs and couple this with the power of virus evolution and selection to screen for host genes that when silenced resulted in greater viral infection in vivo...
  4. pmc The broad-spectrum antiviral functions of IFIT and IFITM proteins
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nat Rev Immunol 13:46-57. 2013
    ..This Review focuses on recent advances in identifying the unique mechanisms of action of IFIT and IFITM proteins, which explain their broad-spectrum activity against the replication, spread and pathogenesis of a range of human viruses...
  5. pmc Immune responses to West Nile virus infection in the central nervous system
    Hyelim Cho
    Departments of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Viruses 4:3812-30. 2012
    ..We will focus on defining the current state of knowledge of WNV entry, tropism, and host immune response in the CNS, all of which affect the balance between injury and successful clearance...
  6. pmc Cell-intrinsic innate immune control of West Nile virus infection
    Michael S Diamond
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Trends Immunol 33:522-30. 2012
    ....
  7. pmc Recent progress in West Nile virus diagnosis and vaccination
    Marina De Filette
    Laboratory of Gene Therapy, Faculty of Veterinary Sciences, Ghent University, Heidestraat 19, 9820 Merelbeke, Belgium
    Vet Res 43:16. 2012
    ..This review describes the molecular virology, epidemiology, pathogenesis, and highlights recent progress regarding diagnosis and vaccination against WNV infections...
  8. pmc A tetravalent dengue nanoparticle stimulates antibody production in mice
    Elisângela F Silva
    Institute of Biomedical Science, Federal University of Alfenas, Minas Gerais, Brazil
    J Nanobiotechnology 10:13. 2012
    ..Currently, there are no licensed vaccines or antiviral drugs to prevent or treat dengue infections. Biodegradable nanoparticles coated with proteins represent a promising method for in vivo delivery of vaccines...
  9. pmc Enhancement of anti-DIII antibodies by the C3d derivative P28 results in lower viral titers and augments protection in mice
    Matthew D Dunn
    Center for Vaccine Research, University of Pittsburgh, 9047 Biomedical Science Tower 3, Pittsburgh, PA 15261, USA
    Virol J 7:95. 2010
    ..In general, mice vaccinated with P28-adjuvated vaccines produced higher IgG titers than mice vaccinated with non-adjuvanted vaccines...
  10. pmc Actively replicating West Nile virus is resistant to cytoplasmic delivery of siRNA
    Brian J Geiss
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    Virol J 2:53. 2005
    ..Here, we examine the ability of exogenous small interfering RNAs (siRNAs) to block the replication of West Nile virus in human cells...
  11. ncbi request reprint A genetic basis for human susceptibility to West Nile virus
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, Box 8051, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Trends Microbiol 14:287-9. 2006
    ..The study highlights a possible risk in targeting this receptor for the prevention and/or treatment of infectious diseases...
  12. ncbi request reprint The host immunologic response to West Nile encephalitis virus
    Michael S Diamond
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA
    Front Biosci (Landmark Ed) 14:3024-34. 2009
    ..This review will focus on the interactions between WNV and the protective and pathogenic host immune responses...
  13. pmc The structural immunology of antibody protection against West Nile virus
    Michael S Diamond
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Immunol Rev 225:212-25. 2008
    ..In this review, we discuss the structural, molecular, and immunologic basis for antibody-mediated protection against WNV, and its implications for novel therapeutic or vaccine strategies...
  14. pmc Progress on the development of therapeutics against West Nile virus
    Michael S Diamond
    Departments of Medicine, Molecular Microbiology, Pathology and Immunology, Washington University School of Medicine, St Louis, MO, United States
    Antiviral Res 83:214-27. 2009
    ..This review describes the current state of knowledge on epidemiology, clinical presentation, pathogenesis, and immunobiology of WNV infection, and highlights progress toward an effective therapy...
  15. ncbi request reprint Development of effective therapies against West Nile virus infection
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, Box 8051, St Louis, MO 63110, USA
    Expert Rev Anti Infect Ther 3:931-44. 2005
    ..This review describes the epidemiology, clinical presentation and pathogenesis of West Nile virus infection, and highlights recent progress towards an effective therapy...
  16. doi request reprint Ebola images emerge from the cave
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Cell Host Microbe 4:87-9. 2008
    ..Recently, the crystal structure of the Ebola virus glycoprotein in complex with a human neutralizing antibody was illuminated, providing a path from the shadows toward understanding cellular attachment, viral fusion, and immune evasion...
  17. pmc B cells and antibody play critical roles in the immediate defense of disseminated infection by West Nile encephalitis virus
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    J Virol 77:2578-86. 2003
    ..We conclude that antibodies and B cells play a critical early role in the defense against disseminated infection by WNV...
  18. pmc A critical role for induced IgM in the protection against West Nile virus infection
    Michael S Diamond
    Washington University School of Medicine, 660 S Euclid Ave, Box 8051, St Louis, MO 63110, USA
    J Exp Med 198:1853-62. 2003
    ..Thus, the induction of a specific, neutralizing IgM response early in the course of WNV infection limits viremia and dissemination into the central nervous system, and protects against lethal infection...
  19. ncbi request reprint Evasion of innate and adaptive immunity by flaviviruses
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri 63110, United States of America
    Immunol Cell Biol 81:196-206. 2003
    ..A more thorough understanding of these principles could lead to improved models for viral pathogenesis and to strategies for the development of novel antiviral agents...
  20. ncbi request reprint Innate and adaptive immune responses determine protection against disseminated infection by West Nile encephalitis virus
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Viral Immunol 16:259-78. 2003
    ..These findings are analyzed in the context of recent approaches to vaccine development and immunotherapy against WNV...
  21. doi request reprint Mechanisms of evasion of the type I interferon antiviral response by flaviviruses
    Michael S Diamond
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Interferon Cytokine Res 29:521-30. 2009
    ..This review will summarize our current understanding of how mammalian cells recognize flaviviruses to induce an inhibitory IFN response and the countermeasures this group of viruses has evolved to antagonize this response...
  22. pmc Interferon regulatory factor IRF-7 induces the antiviral alpha interferon response and protects against lethal West Nile virus infection
    Stephane Daffis
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 82:8465-75. 2008
    ..In contrast, in these cells the IFN-beta response remained largely intact. Our data suggest that the early protective IFN-alpha response against WNV occurs through an IRF-7-dependent transcriptional signal...
  23. pmc Toll-like receptor 3 has a protective role against West Nile virus infection
    Stephane Daffis
    Departments of Medicine, Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 82:10349-58. 2008
    ..Taken together, our data suggest that TLR3 serves a protective role against WNV in part, by restricting replication in neurons...
  24. pmc Early B-cell activation after West Nile virus infection requires alpha/beta interferon but not antigen receptor signaling
    Whitney E Purtha
    Departments of Medicine, Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    J Virol 82:10964-74. 2008
    ....
  25. pmc The relative contribution of antibody and CD8+ T cells to vaccine immunity against West Nile encephalitis virus
    Bimmi Shrestha
    Department of Medicine, 660 S Euclid Avenue, Box 8051, Washington University School of Medicine, St Louis, MO 63110, United States
    Vaccine 26:2020-33. 2008
    ....
  26. pmc Cell-specific IRF-3 responses protect against West Nile virus infection by interferon-dependent and -independent mechanisms
    Stephane Daffis
    Department of Medicine, Washington University School of Medicine, St Louis Missouri, United States of America
    PLoS Pathog 3:e106. 2007
    ..Collectively, our data suggest that cell-specific IRF-3 responses protect against WNV infection through both IFN-dependent and -independent programs...
  27. pmc Tumor necrosis factor alpha protects against lethal West Nile virus infection by promoting trafficking of mononuclear leukocytes into the central nervous system
    Bimmi Shrestha
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    J Virol 82:8956-64. 2008
    ..Our data are most consistent with a model in which interaction of TNF-alpha with TNF-R1 protects against WNV infection by regulating migration of protective inflammatory cells into the brain during acute infection...
  28. pmc Protective immune responses against West Nile virus are primed by distinct complement activation pathways
    Erin Mehlhop
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Exp Med 203:1371-81. 2006
    ..Our results suggest that individual pathways of complement activation control WNV infection by priming adaptive immune responses through distinct mechanisms...
  29. pmc The innate immune adaptor molecule MyD88 restricts West Nile virus replication and spread in neurons of the central nervous system
    Kristy J Szretter
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 84:12125-38. 2010
    ..Our experiments suggest that MyD88 restricts WNV by inhibiting replication in subsets of cells and modulating expression of chemokines that regulate immune cell migration into the central nervous system...
  30. pmc Alpha/beta interferon protects against lethal West Nile virus infection by restricting cellular tropism and enhancing neuronal survival
    Melanie A Samuel
    Department of Molecular Microbiology, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 79:13350-61. 2005
    ..Collectively, our data suggest that IFN-alpha/beta controls WNV infection by restricting tropism and viral burden and by preventing death of infected neurons...
  31. pmc Antibody prophylaxis and therapy against West Nile virus infection in wild-type and immunodeficient mice
    Michael J Engle
    Departments of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 77:12941-9. 2003
    ..Thus, passive transfer of immune antibody improves clinical outcome even after WNV has disseminated into the central nervous system...
  32. pmc Role of CD8+ T cells in control of West Nile virus infection
    Bimmi Shrestha
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Virol 78:8312-21. 2004
    ..Collectively, our experiments suggest that, while specific antibody is responsible for terminating viremia, CD8(+) T cells have an important function in clearing infection from tissues and preventing viral persistence...
  33. pmc Batf3 deficiency reveals a critical role for CD8alpha+ dendritic cells in cytotoxic T cell immunity
    Kai Hildner
    Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Science 322:1097-100. 2008
    ..Importantly, rejection of highly immunogenic syngeneic tumors was impaired in Batf3-/- mice. These results suggest an important role for CD8alpha+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection...
  34. pmc CD40-CD40 ligand interactions promote trafficking of CD8+ T cells into the brain and protection against West Nile virus encephalitis
    Elizabeth Sitati
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Virol 81:9801-11. 2007
    ..Beside its role in maturation of antibody responses, our experiments suggest a novel function of CD40-CD40L interactions: to facilitate T-cell migration across the blood-brain barrier to control WNV infection...
  35. pmc Antibody recognition of cell surface-associated NS1 triggers Fc-gamma receptor-mediated phagocytosis and clearance of West Nile Virus-infected cells
    Kyung Min Chung
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Virol 81:9551-5. 2007
    ..These findings may be relevant for generating novel therapeutic MAbs or vaccines against flaviviruses that target the NS1 protein...
  36. pmc Complement protein C1q inhibits antibody-dependent enhancement of flavivirus infection in an IgG subclass-specific manner
    Erin Mehlhop
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Host Microbe 2:417-26. 2007
    ..IgG subclasses that avidly bind C1q induced minimal ADE in the presence of C1q. These findings add a layer of complexity for the analysis of humoral immunity and flavivirus infection...
  37. pmc Caspase 3-dependent cell death of neurons contributes to the pathogenesis of West Nile virus encephalitis
    Melanie A Samuel
    Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Avenue, St Louis, MO 63110, USA
    J Virol 81:2614-23. 2007
    ..These studies establish that caspase 3-dependent apoptosis contributes to the pathogenesis of lethal WNV encephalitis and suggest possible novel therapeutic targets to restrict CNS injury...
  38. pmc Complement activation is required for induction of a protective antibody response against West Nile virus infection
    Erin Mehlhop
    Department of Medicine, Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
    J Virol 79:7466-77. 2005
    ..Overall, these results suggest that complement controls WNV infection, in part through its ability to induce a protective antibody response...
  39. pmc CD4+ T-cell responses are required for clearance of West Nile virus from the central nervous system
    Elizabeth M Sitati
    Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 80:12060-9. 2006
    ..Our experiments suggest that the dominant protective role of CD4(+) T cells during primary WNV infection is to provide help for antibody responses and sustain WNV-specific CD8(+) T-cell responses in the CNS that enable viral clearance...
  40. pmc Human monoclonal antibodies against West Nile virus induced by natural infection neutralize at a postattachment step
    Matthew R Vogt
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 83:6494-507. 2009
    ..CR4348 and CR4354 human MAbs neutralize infection at a postattachment step in the viral life cycle, likely by inhibiting acid-induced fusion within the endosome...
  41. pmc Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus
    Theodore Oliphant
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, Missouri 63110, USA
    Nat Med 11:522-30. 2005
    ..In postexposure therapeutic trials in mice, a single dose of humanized E16 protected mice against WNV-induced mortality, and may therefore be a viable treatment option against WNV infection in humans...
  42. pmc Induction of IFN-beta and the innate antiviral response in myeloid cells occurs through an IPS-1-dependent signal that does not require IRF-3 and IRF-7
    Stephane Daffis
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Pathog 5:e1000607. 2009
    ....
  43. pmc Defining the levels of secreted non-structural protein NS1 after West Nile virus infection in cell culture and mice
    Kyung Min Chung
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Med Virol 80:547-56. 2008
    ..Overall, the NS1-based capture ELISA is a sensitive readout of infection and could be an important tool for diagnosis or screening small molecule inhibitors of WNV infection...
  44. ncbi request reprint Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
    ..Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases...
  45. ncbi request reprint The molecular basis of antibody-mediated neutralization of West Nile virus
    Theodore Oliphant
    Washington University School of Medicine, Department of Molecular Microbiology, Saint Louis, MO 63110, USA
    Expert Opin Biol Ther 7:885-92. 2007
    ..Antibodies that bind to this epitope are particularly inhibitory because they block infection at a post-attachment step and at concentrations that result in a low occupancy of the available sites on the virion...
  46. doi request reprint Immunological headgear: antiviral immune responses protect against neuroinvasive West Nile virus
    Robyn S Klein
    Department of Internal Medicine, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    Trends Mol Med 14:286-94. 2008
    ..Here, we present our current understanding of the innate and adaptive immune responses that limit dissemination to the CNS from WNV infection and the antiviral immune responses within the CNS that intervene when they fail...
  47. pmc Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc gamma receptor-dependent and -independent mechanisms
    Kyung Min Chung
    Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Avenue, St Louis, MO 63110, USA
    J Virol 80:1340-51. 2006
    ..Overall, our data suggest that distinct regions of NS1 can elicit protective humoral immunity against WNV through different mechanisms...
  48. pmc Structure and function analysis of therapeutic monoclonal antibodies against dengue virus type 2
    Soila Sukupolvi-Petty
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
    J Virol 84:9227-39. 2010
    ..Overall, these studies define the structural and functional complexity of antibodies against DENV-2 with protective potential...
  49. ncbi request reprint Herpesvirus latency confers symbiotic protection from bacterial infection
    Erik S Barton
    Departments of Pathology and Immunology, Washington University Medical School, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nature 447:326-9. 2007
    ..Thus, whereas the immune evasion capabilities and lifelong persistence of herpesviruses are commonly viewed as solely pathogenic, our data suggest that latency is a symbiotic relationship with immune benefits for the host...
  50. pmc Neuronal CXCL10 directs CD8+ T-cell recruitment and control of West Nile virus encephalitis
    Robyn S Klein
    Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Virol 79:11457-66. 2005
    ....
  51. pmc Direct complement restriction of flavivirus infection requires glycan recognition by mannose-binding lectin
    Anja Fuchs
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Host Microbe 8:186-95. 2010
    ..Our studies show that MBL recognizes terminal mannose-containing carbohydrates on flaviviruses, resulting in neutralization and efficient clearance in vivo...
  52. pmc PKR and RNase L contribute to protection against lethal West Nile Virus infection by controlling early viral spread in the periphery and replication in neurons
    Melanie A Samuel
    Department of Molecular Microbiology, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 80:7009-19. 2006
    ..Our data suggest that PKR and RNase L contribute to IFN-mediated protection in a cell-restricted manner and control WNV infection in peripheral tissues and some neuronal subtypes...
  53. pmc Induction of epitope-specific neutralizing antibodies against West Nile virus
    Theodore Oliphant
    Departments of Medicine, Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Virol 81:11828-39. 2007
    ....
  54. pmc Essential role of mda-5 in type I IFN responses to polyriboinosinic:polyribocytidylic acid and encephalomyocarditis picornavirus
    Leonid Gitlin
    Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Campus Box 8118, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 103:8459-64. 2006
    ..Moreover, mda-5-/- mice exhibited a selectively impaired antiviral response to encephalomyocarditis picornavirus, indicating functional specialization of mda-5 in vivo...
  55. pmc Gamma interferon plays a crucial early antiviral role in protection against West Nile virus infection
    Bimmi Shrestha
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
    J Virol 80:5338-48. 2006
    ..Collectively, our experiments suggest that the dominant protective role of IFN-gamma against WNV is antiviral in nature, occurs in peripheral lymphoid tissues, and prevents viral dissemination to the CNS...
  56. pmc A therapeutic antibody against west nile virus neutralizes infection by blocking fusion within endosomes
    Bruce S Thompson
    Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA
    PLoS Pathog 5:e1000453. 2009
    ..Thus, a strongly neutralizing anti-WNV MAb with therapeutic potential is potently inhibitory because it blocks viral fusion and thereby promotes clearance by delivering virus to the lysosome for destruction...
  57. pmc CD8+ T cells require perforin to clear West Nile virus from infected neurons
    Bimmi Shrestha
    Departments of Medicine, Molecular Microbiology, Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
    J Virol 80:119-29. 2006
    ..Taken together, our data suggest that despite the risk of immunopathogenesis, CD8+ T cells use a perforin-dependent mechanism to clear WNV from infected neurons...
  58. pmc Complement modulates pathogenesis and antibody-dependent neutralization of West Nile virus infection through a C5-independent mechanism
    Erin Mehlhop
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Virology 393:11-5. 2009
    ..Overall, these results enhance our mechanistic understanding of how complement controls flavivirus infections...
  59. pmc Single-chain HLA-A2 MHC trimers that incorporate an immundominant peptide elicit protective T cell immunity against lethal West Nile virus infection
    Sojung Kim
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 184:4423-30. 2010
    ....
  60. pmc TNF-alpha-dependent regulation of CXCR3 expression modulates neuronal survival during West Nile virus encephalitis
    Bo Zhang
    Division of Infectious Diseases, Washington University School of Medicine, St Louis, MO 63110, USA
    J Neuroimmunol 224:28-38. 2010
    ..These results suggest that neuronal TNF-alpha expression during WNV encephalitis may be an adaptive response to diminish CXCL10-induced death...
  61. pmc Castanospermine, a potent inhibitor of dengue virus infection in vitro and in vivo
    Kevin Whitby
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    J Virol 79:8698-706. 2005
    ..Overall, our data suggest that castanospermine has a strong antiviral effect on dengue virus infection and warrants further development as a possible treatment in humans...
  62. pmc Infection and injury of neurons by West Nile encephalitis virus
    Bimmi Shrestha
    Departments of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 77:13203-13. 2003
    ....
  63. pmc Complement protein C1q reduces the stoichiometric threshold for antibody-mediated neutralization of West Nile virus
    Erin Mehlhop
    Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Cell Host Microbe 6:381-91. 2009
    ....
  64. pmc Type- and subcomplex-specific neutralizing antibodies against domain III of dengue virus type 2 envelope protein recognize adjacent epitopes
    Soila Sukupolvi-Petty
    Department of Medicine, Washington University School of Medicine, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 81:12816-26. 2007
    ..Overall, our experiments define adjacent and structurally distinct epitopes on DIII of DENV-2 which elicit type-specific, subcomplex-specific, and cross-reactive antibodies with different neutralizing potentials...
  65. pmc Fas ligand interactions contribute to CD8+ T-cell-mediated control of West Nile virus infection in the central nervous system
    Bimmi Shrestha
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Virol 81:11749-57. 2007
    ..Overall, our data suggest that CD8+ T cells also utilize FasL effector mechanisms to contain WNV infection in Fas-expressing neurons in the CNS...
  66. pmc CXCR4 antagonism increases T cell trafficking in the central nervous system and improves survival from West Nile virus encephalitis
    Erin E McCandless
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 105:11270-5. 2008
    ..The benefits of enhanced CD8(+) T cell infiltration suggest that pharmacologic targeting of CXCR4 may have therapeutic utility for the treatment of acute viral infections of the CNS...
  67. pmc Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein
    Theodore Oliphant
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, Saint Louis, MO 63110, USA
    J Virol 80:12149-59. 2006
    ..Nonetheless, DI and DII MAbs protect against WNV infection in mice, albeit with lower efficiency than DIII-specific neutralizing MAbs...
  68. pmc The immune adaptor molecule SARM modulates tumor necrosis factor alpha production and microglia activation in the brainstem and restricts West Nile Virus pathogenesis
    Kristy J Szretter
    Departments of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Virol 83:9329-38. 2009
    ....
  69. pmc Measure and countermeasure: type I IFN (IFN-alpha/beta) antiviral response against West Nile virus
    Stephane Daffis
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Innate Immun 1:435-45. 2009
    ....
  70. pmc Identification of novel small-molecule inhibitors of West Nile virus infection
    Amine O Noueiry
    Apath, LLC, St Louis, MO 63110, USA
    J Virol 81:11992-2004. 2007
    ..Overall, these compounds comprise a novel class of promising inhibitors for therapy against WNV and other flavivirus infections in humans...
  71. pmc Pathogenesis of West Nile Virus infection: a balance between virulence, innate and adaptive immunity, and viral evasion
    Melanie A Samuel
    Division of Infectious Diseases, Department of Molecular Microbiology, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Ave, St Louis, MO 63110, USA
    J Virol 80:9349-60. 2006
  72. ncbi request reprint Antigen-specific cytotoxic T lymphocytes protect against lethal West Nile virus encephalitis
    Whitney E Purtha
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Eur J Immunol 37:1845-54. 2007
    ..Based on this, we conclude that CTL responses against immundominant WNV epitopes confer protective immunity and thus should be targets for inclusion in new vaccines...
  73. pmc The interferon-inducible gene viperin restricts West Nile virus pathogenesis
    Kristy J Szretter
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8051, St Louis, MO 63110, USA
    J Virol 85:11557-66. 2011
    ..Our experiments suggest that viperin restricts the infection of WNV in a tissue- and cell-type-specific manner and may be an important ISG for controlling viral infections that cause CNS disease...
  74. pmc Poorly neutralizing cross-reactive antibodies against the fusion loop of West Nile virus envelope protein protect in vivo via Fcgamma receptor and complement-dependent effector mechanisms
    Matthew R Vogt
    Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    J Virol 85:11567-80. 2011
    ....
  75. pmc Neutralizing monoclonal antibodies against hepatitis C virus E2 protein bind discontinuous epitopes and inhibit infection at a postattachment step
    Michelle C Sabo
    Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, Saint Louis, MO 63110, USA
    J Virol 85:7005-19. 2011
    ..Collectively, these studies help to define the structural and functional complexity of antibodies against HCV E2 protein with neutralizing potential...
  76. pmc Beta interferon controls West Nile virus infection and pathogenesis in mice
    Helen M Lazear
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, MO 63110, USA
    J Virol 85:7186-94. 2011
    ..Collectively, these results suggest that IFN-β controls WNV pathogenesis by restricting infection in key cell types and by modulating T cell regulatory networks...
  77. pmc The naturally attenuated Kunjin strain of West Nile virus shows enhanced sensitivity to the host type I interferon response
    Stephane Daffis
    Department of Medicine, Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St Louis Missouri 63110, USA
    J Virol 85:5664-8. 2011
    ..Thus, WNV(KUN) is attenuated primarily through its enhanced restriction by type I interferon- and IRF-3-dependent mechanisms...
  78. pmc Structural insights into the mechanisms of antibody-mediated neutralization of flavivirus infection: implications for vaccine development
    Theodore C Pierson
    Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, 33 North Drive, Building 33, Room 1E19A 2, Bethesda, MD 20892, USA
    Cell Host Microbe 4:229-38. 2008
    ..These insights have identified factors that govern the potency of neutralizing antibodies and will inform the design and evaluation of novel vaccines...
  79. ncbi request reprint Pathogenesis of flavivirus encephalitis
    Thomas J Chambers
    Department of Molecular Microbiology and Immunology, St Louis University Health Sciences Center, School of Medicine, St Louis, Missouri 63104, USA
    Adv Virus Res 60:273-342. 2003
  80. pmc Molecular mechanisms of antibody-mediated neutralisation of flavivirus infection
    Theodore C Pierson
    Viral Pathogenesis Section, Laboratory of Viral Diseases, NIAID, NIH, Bethesda, MD 20892, USA
    Expert Rev Mol Med 10:e12. 2008
    ..How recent advances in this area have the potential to improve the development of safe and effective vaccines and immunotherapeutics is also addressed...
  81. pmc Maturation of West Nile virus modulates sensitivity to antibody-mediated neutralization
    Steevenson Nelson
    Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 4:e1000060. 2008
    ..Thus, in addition to a role in facilitating viral entry, changes in E protein arrangement associated with maturation modulate neutralization sensitivity and introduce an additional layer of complexity into humoral immunity against WNV...
  82. ncbi request reprint A rapid and quantitative assay for measuring antibody-mediated neutralization of West Nile virus infection
    Theodore C Pierson
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Virology 346:53-65. 2006
    ..WNV RVPs are capable of only a single round of infection, can be used under BSL-2 conditions, and offer a rapid and quantitative approach for detecting virus entry and its inhibition by neutralizing antibody...
  83. pmc West Nile virus-induced acute flaccid paralysis is prevented by monoclonal antibody treatment when administered after infection of spinal cord neurons
    John D Morrey
    Institute for Antiviral Research, Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah 84322 4700, USA
    J Neurovirol 14:152-63. 2008
    ....
  84. pmc Defining limits of treatment with humanized neutralizing monoclonal antibody for West Nile virus neurological infection in a hamster model
    John D Morrey
    Institute for Antiviral Research, Animal, Dairy, and Veterinary Sciences Department, Utah State University, 4700 Old Main Hill, Logan, UT 84322 4700, USA
    Antimicrob Agents Chemother 51:2396-402. 2007
    ..3 microg/ml or higher. Overall, these data suggest that in hamsters hE16 can ameliorate neurological disease after significant viral replication has occurred, although there is a time window that limits therapeutic efficacy...
  85. pmc Protective and therapeutic capacity of human single-chain Fv-Fc fusion proteins against West Nile virus
    L Hannah Gould
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520, USA
    J Virol 79:14606-13. 2005
    ..These recombinant human scFvs are potential candidates for immunoprophylaxis and therapy of flavivirus infections...
  86. pmc The stoichiometry of antibody-mediated neutralization and enhancement of West Nile virus infection
    Theodore C Pierson
    Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Host Microbe 1:135-45. 2007
    ..Thus, neutralization is best described by a model requiring "multiple hits" with the cumulative functional outcome determined by interplay between antibody affinity and epitope accessibility...
  87. ncbi request reprint CXCR3 mediates region-specific antiviral T cell trafficking within the central nervous system during West Nile virus encephalitis
    Bo Zhang
    Division of Infectious Diseases, Children s Hospital of Boston, Boston, MA, USA
    J Immunol 180:2641-9. 2008
    ..These results indicate that WNV differentially induces CXCL10 within neuronal populations and suggest a novel model for nonredundancy in chemokine-mediated inflammation among CNS compartments...
  88. ncbi request reprint Humanized monoclonal antibody against West Nile virus envelope protein administered after neuronal infection protects against lethal encephalitis in hamsters
    John D Morrey
    Institute for Antiviral Research, Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84322 4700, USA
    J Infect Dis 194:1300-8. 2006
    ..These experiments suggest that humanized MAbs with potent neutralizing activity are a possible treatment for human patients after WNV has infected neurons in the central nervous system...
  89. ncbi request reprint An infectious West Nile virus that expresses a GFP reporter gene
    Theodore C Pierson
    Department of Microbiology, University of Pennsylvania, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Virology 334:28-40. 2005
    ..We anticipate this DNA-based, infectious WNV reporter virus will allow novel approaches for the study of WNV infection and its inhibition both in vitro and in vivo...
  90. ncbi request reprint West Nile virus: crossing the blood-brain barrier
    Michael S Diamond
    Nat Med 10:1294-5. 2004
  91. doi request reprint Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins
    Shee Mei Lok
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Struct Mol Biol 15:312-7. 2008
    ..The changes in the structure of the viral surface are presumably responsible for inhibiting attachment to cells...
  92. pmc Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase
    Sergey A Shiryaev
    The Burnham Institute, La Jolla, CA 92037, USA
    Biochem J 393:503-11. 2006
    ..Overall, our findings represent a foundation for in-depth mechanistic and structural studies as well as for the design of novel and efficient inhibitors of WNV NS3...
  93. pmc Axonal transport mediates West Nile virus entry into the central nervous system and induces acute flaccid paralysis
    Melanie A Samuel
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 104:17140-5. 2007
    ..Moreover, antibody therapeutics directly inhibit transneuronal spread of WNV infection and prevent the development of paralysis in vivo...
  94. pmc Crystal structure of the West Nile virus envelope glycoprotein
    Grant E Nybakken
    Department of Pathology and Immunology, Washington University School of Medicine, Campus Box 8118, 660 South Euclid Avenue, St Louis, MO 63110 1093, USA
    J Virol 80:11467-74. 2006
    ..We speculate that E proteins could pivot around the fusion loop-pocket junction, allowing virion conformational transitions while minimizing fusion loop exposure...
  95. pmc Resistance to alpha/beta interferon is a determinant of West Nile virus replication fitness and virulence
    Brian C Keller
    Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
    J Virol 80:9424-34. 2006
    ..Consistent with this finding, the virulence of MAD78 was unmasked upon infection of mice lacking IFNAR. Thus, control of the innate host response and IFN actions is a key feature of WNV pathogenesis and replication fitness...

Research Grants10

  1. Antibody Based Therapeuticcs for Chikungunya Virus
    Michael S Diamond; Fiscal Year: 2010
    ..Overall, the generation of strongly protective humanized MAbs against CHIKV will foster the clinical development of immunotherapeutic agents against CHIKV infection. ..
  2. INTERFERON IN MODULATING DENGUE VIRUS INFECTION
    Michael Diamond; Fiscal Year: 2002
    ....
  3. Antibody-Based Protection Against Dengue Virus
    Michael S Diamond; Fiscal Year: 2010
    ..In Specific Aim 4, we will develop epitope-based diagnostic assays and immunogens for DENV. Collectively, these studies may be of significant importance in ongoing and future DENV vaccine development programs. ..
  4. Antibody-Based Protection Against Dengue Virus
    Michael Diamond; Fiscal Year: 2007
    ..In Specific Aim 4, we will develop epitope-based diagnostic assays and immunogens for DENV. Collectively, these studies may be of significant importance in ongoing and future DENV vaccine development programs. ..
  5. Monoclonal Antibody Therapy for West Nile Virus
    Michael Diamond; Fiscal Year: 2007
    ..These studies are an essential first step in the generation of humanized mAbs that have utility as therapeutic agents against WNV in humans. ..
  6. Development of Novel Antiviral Agents VS West Nile Virus
    Michael Diamond; Fiscal Year: 2006
    ....