Research Topics
Genomes and Genes | E CrouchSummaryAffiliation: Washington University School of Medicine Country: USA Publications
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Publications
Surfactant protein D binds to human immunodeficiency virus (HIV) envelope protein gp120 and inhibits HIV replicationJoseph Meschi
Department of Medicine, Sections of Hematology Oncology, Boston University School of Medicine, EBRC 414, 650 Albany Street, Boston, MA 02118, USA
J Gen Virol 86:3097-107. 2005..SP-D is present in lung and airway fluids, as well as in blood and various mucosal locations, and could, like MBL, play a role in restricting HIV transmission or replication in vivo...- Structure, biologic properties, and expression of surfactant protein D (SP-D)E C Crouch
Department of Pathology, Barnes Jewish Hospital at Washington University Medical Center, 216 S Kingshighway, St Louis, MO 63110, USA
Biochim Biophys Acta 1408:278-89. 1998.... - Ligand specificity of human surfactant protein D: expression of a mutant trimeric collectin that shows enhanced interactions with influenza A virusErika Crouch
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 280:17046-56. 2005..In addition, RAK resembled CL-43 in its greater capacity to inhibit the infectivity of influenza A virus and to increase uptake of influenza by neutrophils... - Contributions of phenylalanine 335 to ligand recognition by human surfactant protein D: ring interactions with SP-D ligandsErika Crouch
Department of Pathology and Immunology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
J Biol Chem 281:18008-14. 2006..Our studies indicate that Phe-335, which is evolutionarily conserved in all known SP-Ds, plays important, if not critical, roles in SP-D function... 
Species differences in the carbohydrate binding preferences of surfactant protein DErika C Crouch
Dept of Pathology and Immunology, Campus Box 8118, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
Am J Respir Cell Mol Biol 35:84-94. 2006..Thus, ligand recognition by human SP-D involves a complex interplay between saccharide presentation, the valency of trimeric subunits, and species-specific residues that flank the primary carbohydrate binding site...- Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary surfactant protein DErika Crouch
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Biochemistry 46:5160-9. 2007..In addition, the studies indicate that oligomerization of trimeric subunits is an important determinant of recognition of PI by human SP-D... - Recognition of mannosylated ligands and influenza A virus by human surfactant protein D: contributions of an extended site and residue 343Erika Crouch
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Biochemistry 48:3335-45. 2009..Our findings demonstrate an extended ligand binding site for mannosylated ligands and the significant contribution of the 343 side chain to specific recognition of multivalent microbial ligands, including high-mannose viral glycans... - Myeloperoxidase-dependent inactivation of surfactant protein D in vitro and in vivoErika C Crouch
Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
J Biol Chem 285:16757-70. 2010..Thus, MPO-derived oxidants can lead to modifications of SP-D structure with associated alterations in its characteristic aggregating activity... 
Surfactant protein-D and pulmonary host defenseE C Crouch
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA
Respir Res 1:93-108. 2000..The expression of SP-D is increased in response to many forms of lung injury, and deficient accumulation of appropriately oligomerized SP-D might contribute to the pathogenesis of a variety of human lung diseases...- Surfactant proteins a and d and pulmonary host defenseE Crouch
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
Annu Rev Physiol 63:521-54. 2001.... - Collectins and pulmonary innate immunityE Crouch
Department of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA
Immunol Rev 173:52-65. 2000.... - Collectins and pulmonary host defenseE C Crouch
Department of Pathology, Washington University School of Medicine, St Louis, Missouri, USA
Am J Respir Cell Mol Biol 19:177-201. 1998..Acquired or genetic alterations in the levels of active proteins within the airspaces and distal airways may increase susceptibility to infection... - Mutagenesis of surfactant protein D informed by evolution and x-ray crystallography enhances defenses against influenza A virus in vivoErika Crouch
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 286:40681-92. 2011..These studies demonstrate the potential utility of homology modeling and protein structure analysis for engineering effective collectin antivirals as in vivo therapeutics... - Molecular structure of pulmonary surfactant protein D (SP-D)E Crouch
Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110
J Biol Chem 269:17311-9. 1994.... - Purification and biochemical characterization of CP4 (SP-D), a collagenous surfactant-associated proteinA Persson
Department of Pathology, Jewish Hospital, Washington University Medical Center, St Louis, Missouri 63110
Biochemistry 28:6361-7. 1989..We propose that CP4 be designated "surfactant protein D" (SP-D) in accordance with an accepted nomenclature for surfactant-associated proteins... - Accumulation of surfactant protein D in human pulmonary alveolar proteinosisE Crouch
Department of Pathology, Jewish Hospital, St Louis, MO 63110
Am J Pathol 142:241-8. 1993..These studies describe the isolation and biochemical characterization of human SP-D and demonstrate the abnormal accumulation of this protein in the air spaces of patients with alveolar proteinosis... - Genomic organization of human surfactant protein D (SP-D). SP-D is encoded on chromosome 10q22.2-23.1E Crouch
Department of Pathology, Jewish Hospital, Washington University Medical Center, St Louis, Missouri 63110
J Biol Chem 268:2976-83. 1993..Fluorescence in situ hybridization of metaphase chromosomes using genomic probes gave selective labeling of 10q22.2-23.1. We speculate that SP-D is encoded at a locus on 10q that includes the genes for surfactant protein A... - The amino-terminal heptad repeats of the coiled-coil neck domain of pulmonary surfactant protein d are necessary for the assembly of trimeric subunits and dodecamersP Zhang
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
J Biol Chem 276:19862-70. 2001..These studies provide strong evidence that the amino-terminal heptad repeats of the neck domain are necessary for the intracellular, trimeric association of SP-D monomers and for the assembly and secretion of functional dodecamers... - Human surfactant protein D: SP-D contains a C-type lectin carbohydrate recognition domainK Rust
Department of Pathology, Jewish Hospital, Washington University Medical Center, St Louis, Missouri 63110
Arch Biochem Biophys 290:116-26. 1991..The studies demonstrate that SP-D is a member of the C-type lectin family, and confirm predicted structural similarities to conglutinin, SP-D, and the serum mannose binding proteins... - Surfactant protein D gene regulation. Interactions among the conserved CCAAT/enhancer-binding protein elementsYanchun He
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 277:19530-7. 2002..Thus, interactions among C/EBP elements in the near-distal promoter can modulate the promoter activity of SP-D... - Processing of type II procollagen amino propeptide by matrix metalloproteinasesNaoshi Fukui
Department of Orthopaedic Surgery, Barnes Jewish Hospital at Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 277:2193-201. 2002.... - IL-4 and IL-13 form a negative feedback circuit with surfactant protein-D in the allergic airway responseAngela Haczku
Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104, USA
J Immunol 176:3557-65. 2006..This negative feedback regulatory circuit could be essential in protecting the airways from inflammatory damage after allergen inhalation... - Identification and characterization of endogenous Langerin ligands in murine extracellular matrixYayoi Tada
Dermatology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Massachusetts, USA
J Invest Dermatol 126:1549-58. 2006..Langerin may have an unanticipated role in cell-matrix interactions that modulate LC development, localization, or function... 
Patterns of neutrophil serine protease-dependent cleavage of surfactant protein D in inflammatory lung diseaseJessica Cooley
Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
J Leukoc Biol 83:946-55. 2008..The findings support a pre-eminent role for neutrophil elastase as the critical protease responsible for SP-D depletion in inflammatory lung disease...- Innate defense against influenza A virus: activity of human neutrophil defensins and interactions of defensins with surfactant protein DKevan L Hartshorn
Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
J Immunol 176:6962-72. 2006..These results are relevant to the early phase of host defense against IAV, and suggest a complex interplay between SP-D and HNPs at sites of active inflammation... - Reduced influenza viral neutralizing activity of natural human trimers of surfactant protein DKevan L Hartshorn
Boston University School of Medicine, Department of Medicine, Boston, MA, USA
Respir Res 8:9. 2007..We recently reported that the Thr/Thr 11 form of SP-D is associated with low serum levels and assembles predominantly as trimers as opposed to the more common multimeric forms of SP-D... - Critical role for cross-linking of trimeric lectin domains of surfactant protein D in antiviral activity against influenza A virusTesfaldet Tecle
Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
Biochem J 412:323-9. 2008..These results show that antiviral activities of SP-D can be reproduced without the N-terminal and collagen domains and that cross-linking of NCRDs is essential for antiviral activity of SP-D with respect to IAV... - Antiviral activity of the long chain pentraxin PTX3 against influenza virusesPatrick C Reading
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
J Immunol 180:3391-8. 2008.... - Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulationDelphine C Malherbe
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
Am J Physiol Lung Cell Mol Physiol 289:L856-66. 2005..This study is the first to demonstrate that SP-D modulates IgE-mediated mast cell functions, which are important in asthma and allergic inflammation... - Interactions of surfactant protein D with fatty acidsNihal S DeSilva
Dept. of Pathology and Immunology, Barnes-Jewish Hospital, North Campus, Box 8118 216 S. Kingshighway, St. Louis, MO 63110, USA
Am J Respir Cell Mol Biol 29:757-70. 2003..We speculate that the binding of SP-D to the fatty acyl chains of surfactant lipids, microbial ligands, or other complex lipids contributes to the diverse biological functions of SP-D in vivo... - Porcine pulmonary collectins show distinct interactions with influenza A viruses: role of the N-linked oligosaccharides in the carbohydrate recognition domainMartin van Eijk
Departments of Biochemistry and Cell Biology, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
J Immunol 171:1431-40. 2003.... - Neutrophil serine proteinases inactivate surfactant protein D by cleaving within a conserved subregion of the carbohydrate recognition domainTim O Hirche
Department of Medicine, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
J Biol Chem 279:27688-98. 2004..Together, our data suggest that neutrophil-derived serine proteinases cleave SP-D at sites of inflammation with potential deleterious effects on its biological functions... - Contributions of the N- and C-terminal domains of surfactant protein d to the binding, aggregation, and phagocytic uptake of bacteriaKevan L Hartshorn
Department of Medicine, Boston University School of Medicine, Boston, Massachussets 02118, USA
Infect Immun 70:6129-39. 2002.... - Impact of neutrophils on antiviral activity of human bronchoalveolar lavage fluidMitchell R White
Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Am J Physiol Lung Cell Mol Physiol 293:L1293-9. 2007..g., as in chronic smoking or cystic fibrosis) may reduce SP-D levels and predispose to IAV infection. In contrast, acute inflammation, as occurs in the early phase of IAV infection, may promote neutrophil-mediated viral clearance... - Distinctive anti-influenza properties of recombinant collectin 43Kevan L Hartshorn
Department of Medicine, Boston University School of Medicine, EBRC 414, 650 Albany Street, Boston, MA 02118, U S A
Biochem J 366:87-96. 2002..These findings could facilitate the development of recombinant collectins with novel antimicrobial properties... - Complementation of pulmonary abnormalities in SP-D(-/-) mice with an SP-D/conglutinin fusion proteinLiqian Zhang
Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
J Biol Chem 277:22453-9. 2002..Our findings also strongly suggest that the molecular mechanisms underlying impaired pulmonary host defense and abnormal lipid metabolism are distinct from those that promote ongoing inflammation and the development of emphysema... - Trimerization of the amino propeptide of type IIA procollagen using a 14-amino acid sequence derived from the coiled-coil neck domain of surfactant protein DAudrey McAlinden
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes-Jewish Hospital, 660 South Euclid Avenue, St. Louis, MO 63110, USA
J Biol Chem 277:41274-81. 2002.... - Intramuscular gene transfer of interleukin-10 reduces neutrophil recruitment and ameliorates lung graft ischemia-reperfusion injuryBenjamin D Kozower
Division of Cardiothoracic Surgery, Washington University School of Medicine, Barnes Jewish Hospital, St Louis, MO, USA
Am J Transplant 2:837-42. 2002..This is associated with a reduction in I/R injury with improved isograft oxygenation and reduced tissue edema. Intramuscular gene transfer may be a useful strategy to reduce clinical l/R injury... - Surfactant protein D expression in normal and pneumonic ovine lungBranka Grubor
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Veterinary Medicine, Ames, IA 50011 1250, USA
Vet Immunol Immunopathol 101:235-42. 2004.... - Human neutrophil defensins increase neutrophil uptake of influenza A virus and bacteria and modify virus-induced respiratory burst responsesTesfaldet Tecle
Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
J Immunol 178:8046-52. 2007.... - A role for surfactant protein D in innate immunity of the human prostateRebecca E Oberley
Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Prostate 65:241-51. 2005..SP-D protein levels are increased at sites of inflammation in the prostate, suggesting SP-D may also contribute more generally to inflammatory regulation in the prostate... - Surfactant protein A and D differently regulate the immune response to nonmucoid Pseudomonas aeruginosa and its lipopolysaccharidePhilip Bufler
Dr. von Haunersches Kinderspital, University of Munich, Munich, Germany
Am J Respir Cell Mol Biol 28:249-56. 2003..aeruginosa of diverse LPS phenotypes, and preferentially augments the cellular response to rough P. aeruginosa endotoxin... - Surfactant protein D increases fusion of Mycobacterium tuberculosis-containing phagosomes with lysosomes in human macrophagesJ Scott Ferguson
Division of Infectious Diseases, Department of Internal Medicine, The Ohio State University, N-1147 Doan Hall, 410 W. 10th Avenue, Columbus, OH 43210, USA
Infect Immun 74:7005-9. 2006..Here we show that SP-D limits the intracellular growth of bacilli in macrophages by increasing phagosome-lysosome fusion but not by generating a respiratory burst... - Effect of lung surfactant collectins on bronchoalveolar macrophage interaction with Blastomyces dermatitidis: inhibition of tumor necrosis factor alpha production by surfactant protein DMadhavi Lekkala
Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center, 751 South Bascom Ave, San Jose, California 95128 2699, USA
Infect Immun 74:4549-56. 2006..dermatitidis may utilize BALF constituents, such as SP-D, to blunt the host defensive reaction; this effect could reduce inflammation and tissue destruction but could also promote disease... - Surfactant proteins A and D enhance the phagocytosis of Chlamydia into THP-1 cellsRebecca E Oberley
Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Am J Physiol Lung Cell Mol Physiol 287:L296-306. 2004..In addition, the chlamydial pathogens internalized in the presence of collectins are able to grow and replicate in the THP-1 cells after phagocytosis... - Enhanced surfactant protein and defensin mRNA levels and reduced viral replication during parainfluenza virus type 3 pneumonia in neonatal lambsBranka Grubor
Department of Veterinary Pathology, 2740 Veterinary Medicine, Iowa State University, Ames, IA 50011 1250, USA
Clin Diagn Lab Immunol 11:599-607. 2004.... - Cutting edge: B and T lymphocyte attenuator and programmed death receptor-1 inhibitory receptors are required for termination of acute allergic airway inflammationChristine Deppong
Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
J Immunol 176:3909-13. 2006..Thus, these receptors are critical determinants of the duration of allergic airway inflammation... - Surfactant protein D-mediated aggregation of Pneumocystis carinii impairs phagocytosis by alveolar macrophagesSuk Joong Yong
Thoracic Diseases Research Unit, Division of Pulmonary, Critical Care, and Internal Medicine, 8 24 Stabile Building, Mayo Clinic, Rochester, MN 55905, USA
Infect Immun 71:1662-71. 2003..Thus, SP-D-mediated aggregation of P. carinii may represent one means by which the organism avoids elimination by the host... - Surfactant protein D is present in the human female reproductive tract and inhibits Chlamydia trachomatis infectionRebecca E Oberley
Department of Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa City, IA 52242, USA
Mol Hum Reprod 10:861-70. 2004..In conclusion, we detected SP-D in the female reproductive tract and determined that one of the functions of the SP-D protein may be to protect cervical epithelial cells from infection by C. trachomatis... - Mycobacterium tuberculosis binding to human surfactant proteins A and D, fibronectin, and small airway epithelial cells under shear conditionsLuanne Hall-Stoodley
Center for Genomic Sciences, Allegheny Singer Research Institute, 320 East North Avenue, 11th Floor, South Tower, Pittsburgh, PA 15212 4772, USA
Infect Immun 74:3587-96. 2006..tuberculosis and host innate immune determinants...
Research Grants
- STRUCTURE/FUNCTION OF SURFACTANT PROTEIN DErika Crouch; Fiscal Year: 2002..abstract_text> ..
- STRUCTURE/FUNCTION OF SURFACTANT PROTEIN DErika Crouch; Fiscal Year: 2006..As in the past, secreted recombinant proteins with novel structural attributes will be characterized and utilized for collaborative studies of SP-D function. ..