John A Cooper

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi request reprint A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration
    L Muhua
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110
    Cell 78:669-79. 1994
  2. pmc The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast
    Wei Lih Lee
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 160:355-64. 2003
  3. pmc The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex
    R A Heil-Chapdelaine
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 151:1337-44. 2000
  4. pmc Vertebrate isoforms of actin capping protein beta have distinct functions In vivo
    M C Hart
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 147:1287-98. 1999
  5. ncbi request reprint Arp2/3 complex: advances on the inner workings of a molecular machine
    J A Cooper
    Department of Cell Biology, Washington University School of Medicine, Saint Louis, MO 63130, USA
    Cell 107:703-5. 2001
  6. ncbi request reprint Control of actin assembly and disassembly at filament ends
    J A Cooper
    Department of Cell Biology, Washington University, Box 8228, St Louis, MO 631110, USA
    Curr Opin Cell Biol 12:97-103. 2000
  7. pmc NudEL targets dynein to microtubule ends through LIS1
    Jun Li
    Department of Cell Biology and Physiology, Washington University in St Louis, School of Medicine, St Louis, MO 63110, USA
    Nat Cell Biol 7:686-90. 2005
  8. pmc Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning
    Melissa D Stuchell-Brereton
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 22:2690-701. 2011
  9. pmc Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance
    Kyoungtae Kim
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 164:567-80. 2004
  10. pmc New insights into mechanism and regulation of actin capping protein
    John A Cooper
    Department of Cell Biology, Washington University, St Louis, MO 63110, USA
    Int Rev Cell Mol Biol 267:183-206. 2008

Collaborators

Detail Information

Publications88

  1. ncbi request reprint A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration
    L Muhua
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110
    Cell 78:669-79. 1994
    ..The phenotype of a double mutant lacking dynein and Act5p is similar to that of single mutants. Therefore, dynactin complex is in the same pathway as dynein and may be necessary for the action of dynein in vivo...
  2. pmc The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast
    Wei Lih Lee
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 160:355-64. 2003
    ..Dynein must remain inactive until microtubule ends interact with the bud cortex, at which time dynein and Pac1 appear to be offloaded from the microtubule to the cortex...
  3. pmc The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex
    R A Heil-Chapdelaine
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 151:1337-44. 2000
    ..These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud...
  4. pmc Vertebrate isoforms of actin capping protein beta have distinct functions In vivo
    M C Hart
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 147:1287-98. 1999
    ..Therefore, CPbeta1 and CPbeta2 each have a distinct function that cannot be provided by the other isoform. CPbeta1 attaches actin filaments to the Z-line, and CPbeta2 organizes the actin at the intercalated discs...
  5. ncbi request reprint Arp2/3 complex: advances on the inner workings of a molecular machine
    J A Cooper
    Department of Cell Biology, Washington University School of Medicine, Saint Louis, MO 63130, USA
    Cell 107:703-5. 2001
    ..A crystal structure, a cryo-EM structure, and a reconstitution of the complex from subunits have been reported. New results also address the nucleation mechanism and the role of bound nucleotide...
  6. ncbi request reprint Control of actin assembly and disassembly at filament ends
    J A Cooper
    Department of Cell Biology, Washington University, Box 8228, St Louis, MO 631110, USA
    Curr Opin Cell Biol 12:97-103. 2000
    ..actin depolymerizing factor/cofilin increases the rate at which actin subunits leave pointed ends, and capping protein caps barbed ends...
  7. pmc NudEL targets dynein to microtubule ends through LIS1
    Jun Li
    Department of Cell Biology and Physiology, Washington University in St Louis, School of Medicine, St Louis, MO 63110, USA
    Nat Cell Biol 7:686-90. 2005
    ..We propose a model in which Ndl1 binds transiently to the plus end to promote targeting of LIS1, which cooperatively recruits dynein...
  8. pmc Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning
    Melissa D Stuchell-Brereton
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 22:2690-701. 2011
    ..In addition, these data provide the first direct evidence that LC occupancy in the dynein motor complex is important for function...
  9. pmc Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance
    Kyoungtae Kim
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 164:567-80. 2004
    ..Actin filaments of patches appear to be nucleated first, then capped with CP. The binding constants of yeast CP for actin suggest that actin capping in yeast is more dynamic than in vertebrates...
  10. pmc New insights into mechanism and regulation of actin capping protein
    John A Cooper
    Department of Cell Biology, Washington University, St Louis, MO 63110, USA
    Int Rev Cell Mol Biol 267:183-206. 2008
    ..In addition, a number of molecules that bind and regulate CP have been discovered, suggesting new ideas for how CP may integrate into diverse processes of cell physiology...
  11. pmc Distinct roles for CARMIL isoforms in cell migration
    Yun Liang
    Department of Cell Biology and Physiology, Washington University, St Louis, MO 63110, USA
    Mol Biol Cell 20:5290-305. 2009
    ..Expression of one CARMIL isoform was not able to rescue the knockdown phenotypes of the other. Thus, the two isoforms are both important for cell migration, but they have distinct functions...
  12. pmc Physiological role of the interaction between CARMIL1 and capping protein
    Marc Edwards
    Department of Cell Biology and Physiology, Washington University in St Louis, St Louis, MO 63110
    Mol Biol Cell 24:3047-55. 2013
    ..One implication is that lamellipodia are only marginally important for cell migration in a wound-healing model. The results also suggest that the ability of CARMIL1 to inhibit CP in cells may be regulated. ..
  13. pmc Identification of a novel inhibitory actin-capping protein binding motif in CD2-associated protein
    Serawit Bruck
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:19196-203. 2006
    ..CD2AP inhibits the function of capping protein in vitro. Therefore, our results support a role of CD2AP in the regulation of the actin cytoskeleton...
  14. pmc Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo
    Meng chi Lin
    Department of Cell Biology and Physiology, Washington University, Saint Louis, MO 63110, USA
    J Cell Sci 123:1329-42. 2010
    ..Together, the results suggest that all three proteins work together to promote actin disassembly, but not in a simple way, and not with equal importance...
  15. pmc Dynactin function in mitotic spindle positioning
    Jeffrey K Moore
    Department of Cell Biology and Physiology, Washington University, Saint Louis, MO 63110, USA
    Traffic 9:510-27. 2008
    ..Interactions among the subunits support a molecular model that resembles the current model for brain dynactin in many respects; however, three subunits at the pointed end of brain dynactin appear to be absent from yeast...
  16. pmc Yeast actin patches are networks of branched actin filaments
    Michael E Young
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 166:629-35. 2004
    ..Our results are consistent with Arp2/3 complex-mediated actin polymerization driving yeast actin patch assembly and motility, as described by a variation of the dendritic nucleation model...
  17. pmc The offloading model for dynein function: differential function of motor subunits
    Wei Lih Lee
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 168:201-7. 2005
    ..Localization of HC to cortical dots depends on both IC and LIC. Thus, the IC and LIC accessory chains have different but essential roles in dynein function, providing new insight into the offloading model...
  18. pmc CD2AP links cortactin and capping protein at the cell periphery to facilitate formation of lamellipodia
    Jianping Zhao
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA
    Mol Cell Biol 33:38-47. 2013
    ..Our data demonstrate how the interplay between specialized actin regulatory molecules shapes the actin cytoskeleton...
  19. pmc The spindle position checkpoint requires positional feedback from cytoplasmic microtubules
    Jeffrey K Moore
    Department of Cell Biology and Physiology, Washington University in St Louis, St Louis, MO 63110, USA
    Curr Biol 19:2026-30. 2009
    ....
  20. pmc A novel pathway that coordinates mitotic exit with spindle position
    Scott A Nelson
    Department of Cell Biology, Washington University in St Louis, St Louis, MO 63110, USA
    Mol Biol Cell 18:3440-50. 2007
    ..Each component of this pathway is required for the spindle position checkpoint to delay mitotic exit until the spindle is positioned correctly...
  21. pmc Neurodegeneration mutations in dynactin impair dynein-dependent nuclear migration
    Jeffrey K Moore
    Department of Cell Biology and Physiology, Washington University, Saint Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 106:5147-52. 2009
    ..The need for this function appears to be context-dependent, and we speculate that CAP-Gly activity may only be necessary when dynein needs to overcome high force thresholds to produce movement...
  22. pmc Molecular analysis of Arp2/3 complex activation in cells
    Brian J Galletta
    Department of Cell Biology and Physiology, Washington University, Saint Louis, MO, USA
    Biophys J 103:2145-56. 2012
    ..Rather our data suggests that these regulators may be playing more subtle roles in establishing functional networks in vivo...
  23. pmc Coordinating mitosis with cell polarity: Molecular motors at the cell cortex
    Jeffrey K Moore
    Dept of Cell Biology and Physiology, Washington University, St Louis, MO 63110, United States
    Semin Cell Dev Biol 21:283-9. 2010
    ....
  24. pmc Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae
    T S Karpova
    Department of Cell Biology and Physiology, Washington University, St Louis, Missouri 63110, USA
    Mol Biol Cell 11:1727-37. 2000
    ..This effect is similar to that of latrunculin, suggesting that Myo2p interacts with filamentous actin. However, inhibition of Myo2p function by expression of its tail domain completely abolished growth...
  25. pmc Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates
    D A Schafer
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110
    J Cell Biol 127:453-65. 1994
    ..Thus, vertebrates have developed isoforms of capping protein that associate with distinct actin-filament arrays...
  26. pmc A cytokinesis checkpoint requiring the yeast homologue of an APC-binding protein
    L Muhua
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 393:487-91. 1998
    ..Another mutation affects Stt4, a phosphatidylinositol-4-OH kinase. Cold temperature is an environmental stimulus that causes misalignment of the mitotic spindle in yeast and appears to activate this checkpoint mechanism...
  27. pmc Binding of myotrophin/V-1 to actin-capping protein: implications for how capping protein binds to the filament barbed end
    Nandini Bhattacharya
    Department of Cell Biology and Physiology, Washington University, St Louis, Missouri 63110, USA
    J Biol Chem 281:31021-30. 2006
    ..This model is also supported by molecular dynamics simulations of CP reported here. The existence of the wobble state may be important for actin dynamics in cells...
  28. pmc Mechanism for CARMIL protein inhibition of heterodimeric actin-capping protein
    Taekyung Kim
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 287:15251-62. 2012
    ..Thus, CARMIL promotes uncapping by binding to a freely accessible site on CP bound to a filament barbed end and inducing a change in the conformation of the actin-binding surface of CP...
  29. pmc Effect of Fgd1 on cortactin in Arp2/3 complex-mediated actin assembly
    Kyoungtae Kim
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 43:2422-7. 2004
    ..Moreover, if Fgd1 is multimerized, then cortactin's activity should be enhanced. Fgd1 and cortactin may participate as scaffolds and signal transducers in a positive feedback cycle to promote actin assembly at the cell cortex...
  30. pmc Src phosphorylation of cortactin enhances actin assembly
    Shandiz Tehrani
    Department of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 104:11933-8. 2007
    ..The adaptor Nck is an important component of the system, linking phosphorylated cortactin with neuronal WASp (N-WASp) and WASp-interacting protein (WIP) to activate Arp2/3 complex...
  31. pmc Actin filaments in yeast are unstable in the absence of capping protein or fimbrin
    T S Karpova
    Department of Cell Biology and Physiology, Washington University Medical School, St Louis, Missouri, USA
    J Cell Biol 131:1483-93. 1995
    ..1995. Cell. 81:591-600). In addition, we find that the unpolymerized pool of actin in yeast is very small relative to that found in higher cells, which suggests that actin filament assembly is less dynamic in yeast than higher cells...
  32. pmc Actin-based motility during endocytosis in budding yeast
    Kyoungtae Kim
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 17:1354-63. 2006
    ..Thus, the molecular requirements for Arp2/3-based actin assembly differ in early versus later stages of endocytosis. Finally, acute loss of actin cables led to increased patch motility...
  33. pmc The interaction of capping protein with the barbed end of the actin filament
    Taekyung Kim
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mol Biol 404:794-802. 2010
    ..These studies provide us with new molecular insight into how CP binds to the actin filament...
  34. ncbi request reprint Integration of signals to the Arp2/3 complex
    Alissa M Weaver
    Department of Cell Biology, Washington University School of Medicine, St Louis, MO 63130, USA
    Curr Opin Cell Biol 15:23-30. 2003
    ..Cortactin also binds the Arp2/3 complex via an A domain; however, it also binds to actin filaments, which helps activate the Arp2/3 complex and stabilise the newly created branches between the filaments...
  35. doi request reprint Roles for actin assembly in endocytosis
    Olivia L Mooren
    Department of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
    Annu Rev Biochem 81:661-86. 2012
    ..We focus mainly on new findings on the relative importance of actin in clathrin-mediated endocytosis (CME) in yeast versus mammalian cells...
  36. pmc A novel role for the GTPase-activating protein Bud2 in the spindle position checkpoint
    Scott A Nelson
    Department of Cell Biology and Physiology, Washington University, Saint Louis, Missouri, USA
    PLoS ONE 7:e36127. 2012
    ..Tem1 is a Ras-like protein and is the critical regulator of mitotic exit, sitting atop the mitotic exit network (MEN). Tem1 is a likely target for Bud2, supported by genetic analyses that exclude other Ras-like proteins...
  37. pmc The spindle position checkpoint is coordinated by the Elm1 kinase
    Jeffrey K Moore
    Department of Cell Biology and Physiology, Washington University, St Louis, MO 63110, USA
    J Cell Biol 191:493-503. 2010
    ..Furthermore, we show that Elm1 promotes the activity of the checkpoint kinase Kin4. These findings reveal a novel function for Elm1 in the SPC and suggest how checkpoint activity may be linked to cellular organization...
  38. pmc Distinct roles for Arp2/3 regulators in actin assembly and endocytosis
    Brian J Galletta
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Biol 6:e1. 2008
    ..Knowledge of these roles provides insight into the in vivo relevance of the dendritic nucleation model for actin assembly...
  39. ncbi request reprint Variant cDNAs encoding proteins similar to the alpha subunit of chicken CapZ
    J A Cooper
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri
    Cell Motil Cytoskeleton 18:204-14. 1991
    ..alpha 2 has four poly(A)+RNA transcripts, one of which is rare in liver. alpha 1 has two transcripts. alpha 1 and alpha 2 are encoded by different single-copy genes by Southern analysis of chicken genomic DNA...
  40. ncbi request reprint Cortactin promotes and stabilizes Arp2/3-induced actin filament network formation
    A M Weaver
    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA
    Curr Biol 11:370-4. 2001
    ..By these mechanisms, cortactin may promote the formation and stabilization of the actin network that drives protrusion at the leading edge of migrating cells...
  41. pmc The unusual dynamics of parasite actin result from isodesmic polymerization
    Kristen M Skillman
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    Nat Commun 4:2285. 2013
    ..Our findings expand the repertoire of mechanisms by which actin polymerization is governed and offer clues about the evolution of self-assembling, stabilized protein polymers. ..
  42. ncbi request reprint Interaction of cortactin and N-WASp with Arp2/3 complex
    Alissa M Weaver
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Biol 12:1270-8. 2002
    ..Cortactin and N-WASp activate Arp2/3 complex, alone or in concert. Both cortactin and N-WASp contain an acidic (A) domain that is required for Arp2/3 complex binding...
  43. pmc Distinct roles for the actin nucleators Arp2/3 and hDia1 during NK-mediated cytotoxicity
    Boyd Butler
    Department of Cell Biology and Physiology, Washington University in St Louis, MO 63110, USA
    Curr Biol 19:1886-96. 2009
    ..Several actin nucleators, including Arp2/3 and various formins, control numerous cytoskeletal-based functions in vivo...
  44. pmc Checkpoint control of mitotic exit--do budding yeast mind the GAP?
    John A Cooper
    Department of Cell Biology, Washington University, St Louis, MO 63110, USA
    J Cell Biol 172:331-3. 2006
    ..Their results call into question the GTP/GDP switch model for Tem1 activity, as have other results in the past. The paper also focuses attention on the two spindle pole bodies as potential sites for regulation of Tem1...
  45. ncbi request reprint Capping protein: new insights into mechanism and regulation
    Martin A Wear
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MI 63110, USA
    Trends Biochem Sci 29:418-28. 2004
    ....
  46. pmc Actin and endocytosis: mechanisms and phylogeny
    Brian J Galletta
    Department of Cell Biology, Washington University Medical School, St Louis, MO, USA
    Curr Opin Cell Biol 21:20-7. 2009
    ..The conservation of the components, across a wide range eukaryotic species, suggests that the partnership between endocytosis and actin may be evolutionarily ancient...
  47. ncbi request reprint Septins have a dual role in controlling mitotic exit in budding yeast
    Guillaume A Castillon
    Department of Cell Biology, Washington University, St Louis, MO 63110, USA
    Curr Biol 13:654-8. 2003
    ..These results suggest that septins serve as a diffusion barrier for Lte1p, and that Cdc10p is needed for the septin ring to serve as a scaffold for a putative microtubule sensor...
  48. pmc Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast
    J F Amatruda
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110
    J Cell Biol 119:1151-62. 1992
    ..In addition, bulk secretion of invertase was unimpaired. These data indicate that actin cables are not required for polarized secretion in S. cerevisiae...
  49. ncbi request reprint Actin dynamics: tropomyosin provides stability
    John A Cooper
    Department of Cell Biology, Washington University School of Medicine, Campus Box 8228, 660 S Euclid Ave, St Louis, MO 63110, USA
    Curr Biol 12:R523-5. 2002
    ..Tropomyosin stabilizes actin filaments, while ADF/cofilin promotes their disassembly. These two proteins compete with each other for binding to the actin filament, providing a critical balance for actin assembly in vivo...
  50. ncbi request reprint How capping protein binds the barbed end of the actin filament
    Martin A Wear
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Biol 13:1531-7. 2003
    ..CP with either tentacle alone can cap, as can the isolated beta tentacle alone, suggesting that the individual tentacles interact with more than one actin subunit at a subunit interface at the barbed end...
  51. pmc Capping protein binding to S100B: implications for the tentacle model for capping the actin filament barbed end
    Martin A Wear
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:14382-90. 2004
    ....
  52. pmc Actin dynamics and endocytosis in yeast and mammals
    Brian J Galletta
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Opin Biotechnol 21:604-10. 2010
    ..This review focuses on the regulation of the actin cytoskeleton during CME in yeast and the emerging evidence for a role for actin during mammalian CME...
  53. pmc Function of dynein in budding yeast: mitotic spindle positioning in a polarized cell
    Jeffrey K Moore
    Department of Cell Biology and Physiology, Washington University, St Louis, Missouri, USA
    Cell Motil Cytoskeleton 66:546-55. 2009
    ..Cell Motil. Cytoskeleton 2009. (c) 2009 Wiley-Liss, Inc...
  54. ncbi request reprint The role of Saccharomyces cerevisiae coronin in the actin and microtubule cytoskeletons
    R A Heil-Chapdelaine
    Department of Cell Biology and Physiology Washington University School of Medicine 660 South Euclid Avenue Box 8228, St Louis, Missouri, 63110, USA
    Curr Biol 8:1281-4. 1998
    ..Taken together, these results suggest that S. cerevisiae coronin is a component of the actin cytoskeleton that may interact with the microtubule cytoskeleton...
  55. pmc Dynein-dependent movements of the mitotic spindle in Saccharomyces cerevisiae Do not require filamentous actin
    R A Heil-Chapdelaine
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Biol Cell 11:863-72. 2000
    ..Finally, dynein-dependent sliding of astral microtubules along the cortex and oscillation of the elongating mitotic spindle in the neck occurred in the absence of filamentous actin...
  56. pmc Visualization and molecular analysis of actin assembly in living cells
    D A Schafer
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 143:1919-30. 1998
    ..We conclude that spots, which appear as small projections on the surface by whole mount electron microscopy, represent sites of actin assembly where local and transient changes in the cortical actin cytoskeleton take place...
  57. pmc The surveillance mechanism of the spindle position checkpoint in yeast
    N R Adames
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 153:159-68. 2001
    ..In both wild-type and mutant cells, mitotic exit was preceded by loss of cytoplasmic microtubules from the neck. Thus, the spindle position checkpoint may monitor such interactions...
  58. pmc Actin organization, bristle morphology, and viability are affected by actin capping protein mutations in Drosophila
    R Hopmann
    Department of Biology, Washington University, St Louis, Missouri 63130, USA
    J Cell Biol 133:1293-305. 1996
    ..Our data demonstrate that CP has an essential function during development, and further suggest that CP is required to regulate actin assembly during the development of specialized structures that depend on actin for their morphology...
  59. pmc Rapid and efficient purification of actin from nonmuscle sources
    D A Schafer
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cell Motil Cytoskeleton 39:166-71. 1998
    ..This method for purifying nonmuscle actin will be useful to investigate functional differences in the biology of actin isoforms or their regulating proteins...
  60. pmc Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides
    D A Schafer
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 135:169-79. 1996
    ..A major hypothesis for why capping protein beta-subunit isoforms exist is thereby excluded. Fourth, the proposed capping protein regulators, Hsc70 and S100, had no effect on capping protein binding to actin in vitro...
  61. ncbi request reprint Mapping of the mouse actin capping protein alpha subunit genes and pseudogenes
    M C Hart
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Genomics 39:264-70. 1997
    ..One mouse mutation, de, maps in the vicinity of the alpha 1 gene. No known mouse mutations map to regions near the alpha 2 or alpha 3 genes...
  62. ncbi request reprint Vertebrates have conserved capping protein alpha isoforms with specific expression patterns
    M C Hart
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cell Motil Cytoskeleton 38:120-32. 1997
    ..Most tissues have both alpha1 and alpha2 isoforms but the ratio of alpha1:alpha2 varies widely. Together, these findings support the hypothesis that the CP alpha isoforms have conserved, unique and essential roles in vertebrates...
  63. pmc Quantitative analysis of actin patch movement in yeast
    A E Carlsson
    Department of Physics, Washington University, St Louis, Missouri 63130, USA
    Biophys J 82:2333-43. 2002
    ..A small number of patches in the latrunculin-treated cells display directed motion...
  64. pmc End versus side branching by Arp2/3 complex
    A E Carlsson
    Department of Physics, Washington University, St Louis, Missouri 63130, USA
    Biophys J 86:1074-81. 2004
    ..Another side-branching model, based on aging of subunits' capacity for branch formation, gives a significantly better fit than the end-plus-side model. We discuss implications for actin polymerization in cells...
  65. pmc Depolarization of the actin cytoskeleton is a specific phenotype in Saccharomyces cerevisiae
    T S Karpova
    Department of Cell Biology and Physiology, Washington University, St Louis, MO 63110, USA
    J Cell Sci 111:2689-96. 1998
    ..We conclude that depolarization of the actin cytoskeleton is a specific phenotype...
  66. ncbi request reprint The immunological synapse and the actin cytoskeleton: molecular hardware for T cell signaling
    M L Dustin
    Department of Pathology and Immunology, Washington University School of Medicine, 660 S Euclid Ave, St Louis MO 63110, USA
    Nat Immunol 1:23-9. 2000
    ..This review focuses on the recent convergence of cell biology and immunology studies to explain the role of the actin cytoskeleton in creating the molecular basis for immunological synapse formation and T cell signaling...
  67. pmc Differently phosphorylated forms of the cortactin homolog HS1 mediate distinct functions in natural killer cells
    Boyd Butler
    Department of Cell Biology and Physiology, Washington University, Saint Louis, Missouri 63110, USA
    Nat Immunol 9:887-97. 2008
    ..Thus, HS1 is essential for signaling and actin assembly in natural killer cells, and the functions of the two phosphorylated tyrosine residues are distinct and separable...
  68. ncbi request reprint TOR signaling regulates microtubule structure and function
    J H Choi
    Departments of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA
    Curr Biol 10:861-4. 2000
    ..Our data suggest a role for TOR signaling in regulating microtubule stability and function, possibly through Bik1p...
  69. pmc Regulation of the Wnt signaling pathway by disabled-2 (Dab2)
    B A Hocevar
    Department of Cell Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    EMBO J 22:3084-94. 2003
    ..Based on these results, we propose that Dab2 functions as a negative regulator of canonical Wnt signaling by stabilizing the beta-catenin degradation complex, which may contribute to its proposed role as a tumor suppressor...
  70. ncbi request reprint Uncoupling of membrane ruffling and pinocytosis during Ras signal transduction
    G Li
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 272:10337-40. 1997
    ..Our data indicate that Ha-Ras(G12V) separately activates Rab5-dependent pinocytosis and Rac1-dependent membrane ruffling...
  71. pmc The Sur7p family defines novel cortical domains in Saccharomyces cerevisiae, affects sphingolipid metabolism, and is involved in sporulation
    Michael E Young
    Department of Cell Biology and Physiology, Washington University, St Louis, Missouri 63110, USA
    Mol Cell Biol 22:927-34. 2002
    ..In particular, the sphingoid base length and number of hydroxyl groups in inositol phosphorylceramides were altered in sur7Delta, ydl222Delta, and yne194Delta strains...
  72. pmc Cortactin has an essential and specific role in osteoclast actin assembly
    Shandiz Tehrani
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 17:2882-95. 2006
    ..Cortactin was found to be an early component of the nascent podosome belt, along with dynamin, supporting a role for cortactin in actin assembly...
  73. pmc Immortalized human cerebral microvascular endothelial cells maintain the properties of primary cells in an in vitro model of immune migration across the blood brain barrier
    Brian P Daniels
    Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, MO 63110, USA
    J Neurosci Methods 212:173-9. 2013
    ..Together, these findings demonstrate that HCMEC/D3 is a valid and powerful tool for less expensive and higher throughput in vitro investigations of immune migration at the BBB...
  74. pmc Biological role and structural mechanism of twinfilin-capping protein interaction
    Sandra Falck
    Program in Cellular Biotechnology, Institute of Biotechnology, University of Helsinki, Finland
    EMBO J 23:3010-9. 2004
    ..Our results support a novel model for how sequential interactions between actin monomers, twinfilin, CP, and actin filaments promote cytoskeletal dynamics...
  75. ncbi request reprint Actin capping protein: an essential element in protein kinase signaling to the myofilaments
    W Glen Pyle
    Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Ill 60612, USA
    Circ Res 90:1299-306. 2002
    ....
  76. ncbi request reprint Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility
    James E Bear
    Massachusetts Institute of Technology, Department of Biology, Cambridge 02139, USA
    Cell 109:509-21. 2002
    ..We conclude that Ena/VASP regulates cell motility by controlling the geometry of actin filament networks within lamellipodia...
  77. pmc The pleckstrin homology domain-containing protein CKIP-1 is involved in regulation of cell morphology and the actin cytoskeleton and interaction with actin capping protein
    David A Canton
    Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Mol Cell Biol 25:3519-34. 2005
    ..Overall, our results are consistent with CKIP-1 playing a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein...
  78. pmc Mammalian CARMIL inhibits actin filament capping by capping protein
    Changsong Yang
    Biology Department, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Cell 9:209-21. 2005
    ..This phenotype is reversed by full-length mCARMIL but not mCARMIL lacking the domain that binds CP. Thus, mCARMIL is a key regulator of CP and has profound effects on cell behavior...
  79. pmc Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck
    Brian K Haarer
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E Adams St, Syracuse, NY 13210, USA
    Eukaryot Cell 6:797-807. 2007
    ....
  80. pmc Structure/function analysis of the interaction of phosphatidylinositol 4,5-bisphosphate with actin-capping protein: implications for how capping protein binds the actin filament
    Kyoungtae Kim
    Department of Cell Biology and Physiology
    J Biol Chem 282:5871-9. 2007
    ....
  81. pmc The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein
    David A Canton
    Regulatory Biology and Functional Genomics Research Group, Siebens Drake Medical Research Institute, Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 281:36347-59. 2006
    ....
  82. pmc Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc
    Christopher T Pappas
    Departments of Cell Biology and Anatomy and Molecular and Cellular Biology, The University of Arizona, Tucson, AZ 85721 0106, USA
    Mol Biol Cell 19:1837-47. 2008
    ..We propose a novel molecular model of Z-disc architecture in which nebulin interacts with CapZ from a thin filament of an adjacent sarcomere, thus providing a structural link between sarcomeres...
  83. ncbi request reprint Cortactin interacts with WIP in regulating Arp2/3 activation and membrane protrusion
    Andrew W Kinley
    Department of Microbiology, Health Sciences Center, University of Virginia, Charlottesville, VA 22908, USA
    Curr Biol 13:384-93. 2003
    ..A key regulator of actin polymerization is Arp2/3 complex. Cortactin, an F-actin binding protein that localizes to membrane ruffles, is an activator of Arp2/3 complex...
  84. pmc Pn-AMP1, a plant defense protein, induces actin depolarization in yeasts
    Ja Choon Koo
    Division of Applied Life Science BK21 program, PMBBRC, and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University, Jinju 660 701, Korea
    Plant Cell Physiol 45:1669-80. 2004
    ..Taken together, these results indicate that cell wall proteins are determinants of resistance to Pn-AMP1, and the ability of a plant defense protein to induce actin depolarization is important for its antifungal activity...
  85. ncbi request reprint Dynamin2 and cortactin regulate actin assembly and filament organization
    Dorothy A Schafer
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    Curr Biol 12:1852-7. 2002
    ..GTP hydrolysis altered the organization of the filaments and the lipid vesicles. We conclude that dynamin2, through an interaction with cortactin, regulates actin assembly and actin filament organization at membranes...
  86. pmc Go ahead, break my symmetry!
    Kendall J Blumer
    Nat Cell Biol 5:1048-9. 2003
  87. pmc Tropomyosin regulates elongation by formin at the fast-growing end of the actin filament
    Barbara Wawro
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Biochemistry 46:8146-55. 2007
    ..This role, in addition to its cooperative control of myosin function, establishes tropomyosin as a universal regulator of the multifaceted actin cytoskeleton...