Research Topics
Species | J Perren CobbSummaryAffiliation: Washington University School of Medicine Country: USA Publications
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Publications
Fundamentals of surgical research course: functional genomicsJ Perren Cobb
Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University in St. Louis, St. Louis, MO 63110, USA
J Surg Res 128:194-8. 2005
Validation of the riboleukogram to detect ventilator-associated pneumonia after severe injuryJ Perren Cobb
Center for Critical Illness and Health Engineering, Washington University in St Louis, MO, USA
Ann Surg 250:531-9. 2009..We hypothesized that circulating leukocyte RNA profiles or “riboleukograms” detect ventilator-associated pneumonia after blunt trauma...
The United States critical illness and injury trials group: an introductionJ Perren Cobb
Center for Critical Illness and Health Engineering, Washington University in St Louis, St Louis, Missouri, USA
J Trauma 67:S159-60. 2009....- Injury research in the genomic eraJ Perren Cobb
Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University in St Louis, St Louis, Missouri 63110, USA
Lancet 363:2076-83. 2004..It will now be important to determine how these recent technological advances can be used and integrated with our existing approaches, to reduce death, disability, and the economic consequences of trauma... - The Fourth National Institutes of Health Symposium on the Functional Genomics of Critical Injury: Surviving stress from organ systems to moleculesJ Perren Cobb
Center for Critical Illness and Health Engineering and the Department of Surgery, Washington University in St Louis, MO, USA
Crit Care Med 36:2905-11. 2008..Next steps for the community and suggestions for future research are presented... - Forging a critical alliance: Addressing the research needs of the United States critical illness and injury communityJ Perren Cobb
Center for Critical Illness and Health Engineering, Washington University, St Louis, MO, USA
Crit Care Med 37:3158-60. 2009..Discuss the research needs of the critical illness and injury communities in the United States... - Splenic CD4+ T cells have a distinct transcriptional response six hours after the onset of sepsisJonathan E McDunn
Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
J Am Coll Surg 203:365-75. 2006..CONCLUSIONS: These data demonstrated a sepsis-specific transcriptional program that precedes sepsis-induced phenotypic changes in CD4+ splenocytes... - Cell-specific expression and pathway analyses reveal alterations in trauma-related human T cell and monocyte pathwaysKrzysztof Laudanski
Department of Surgery, University of Rochester School of Medicine, Rochester, NY 14642, USA
Proc Natl Acad Sci U S A 103:15564-9. 2006..Importantly, application of cell separation, genome-wide expression, and cell-specific pathway analyses can be used to discover pathway alterations in human disease... - Surviving sepsis: bcl-2 overexpression modulates splenocyte transcriptional responses in vivoTracey H Wagner
Department of Anesthesiology, School of Medicine, Washington University in St Louis, St Louis, MO 63110, USA
Am J Physiol Regul Integr Comp Physiol 292:R1751-9. 2007..In addition, our data suggest that the ability of bcl-2 overexpression to improve survival in sepsis in this model is related in part to prevention of sepsis-induced alterations in spleen transcriptional responses... - A network-based analysis of systemic inflammation in humansSteve E Calvano
Department of Surgery, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
Nature 437:1032-7. 2005..These findings provide insight into the regulation of global leukocyte activities as they relate to innate immune system tolerance and increased susceptibility to infection in humans... - Commonality and differences in leukocyte gene expression patterns among three models of inflammation and injuryBernard H Brownstein
Department of Genetics, Washington University, St. Louis, Missouri, USA
Physiol Genomics 24:298-309. 2006..Although there is some commonality among the models, the majority of the differentially expressed genes appear to be uniquely associated with the type of injury and/or the inflammatory stimulus... - A genomic storm in critically injured humansWenzhong Xiao
Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
J Exp Med 208:2581-90. 2011..Based on these transcriptional data, we propose a new paradigm for the human immunological response to severe injury... - Molecular diagnostics in sepsis: from bedside to benchT Philip Chung
Department of Surgery, School of Medicine, Washington University, St Louis, MO 63110, USA
J Am Coll Surg 203:585-598. 2006..CONCLUSIONS: Sepsis induces changes in mouse leukocyte gene expression that can be used to diagnose sepsis apart from systemic inflammation... - Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in miceJames A Lederer
Department of Surgery, Brigham and Women s Hospital Harvard Medical School, Boston, Massachusetts 02115, USA
Physiol Genomics 32:299-310. 2008.... - Streptococcus pneumoniae and Pseudomonas aeruginosa pneumonia induce distinct host responsesKevin W McConnell
Departments of Surgery, Washington University School of Medicine, St Louis, MO, USA
Crit Care Med 38:223-41. 2010..The purpose of this study was to determine whether host response is similar after disparate infections with similar mortalities... - Physiologic genomicsJonathan E McDunn
Departments of Surgery, Genetics and Medicine, The Siteman Cancer Center, and Proteomics Center, Washington University School of Medicine, Saint Louis, MO 63110, USA
Surgery 139:133-9. 2006 - Plasticity of the systemic inflammatory response to acute infection during critical illness: development of the riboleukogramJonathan E McDunn
Center for Critical Illness and Health Engineering, Department of Surgery, Washington University in St Louis, St Louis, Missouri, USA
PLoS ONE 3:e1564. 2008..We hypothesized that circulating leukocyte transcriptional profiles can be used to monitor the host response to and recovery from infection complicating critical illness... - Application of genome-wide expression analysis to human health and diseaseJ Perren Cobb
Department of Surgery, Washington University, St. Louis, MO 63130, USA
Proc Natl Acad Sci U S A 102:4801-6. 2005.... - Using systems biology to simplify complex disease: immune cartographyAshoka D Polpitiya
Department of Surgery, Washington University in St Louis, St Louis, MO, USA
Crit Care Med 37:S16-21. 2009..Large, prospective clinical trails are needed to validate the clinical utility of leukocyte RNA diagnostics (e.g., the riboleukogram)... - Clinical microfluidics for neutrophil genomics and proteomicsKenneth T Kotz
Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
Nat Med 16:1042-7. 2010.... - Myocardial transcriptional profiles in a murine model of sepsis: evidence for the importance of agePaul A Checchia
Center for Critical Illness and Health Engineering and the Departments of Pediatrics and Surgery, Washington University School of Medicine, St Louis, MO, USA
Pediatr Crit Care Med 9:530-5. 2008..We hypothesized that analysis of cardiac RNA expression profiles may differentiate between the myocardial response to sepsis in young and old mice... - Whole blood and leukocyte RNA isolation for gene expression analysesRobert J Feezor
Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, USA
Physiol Genomics 19:247-54. 2004..The findings suggest that the method of RNA isolation from whole blood is a critical variable in the design of clinical studies using microarray analyses... - Evidence for a novel blood RNA diagnostic for pediatric appendicitis: the riboleukogramJared T Muenzer
Center for Critical Illness and Health Engineering, Washington University School of Medicine, St Louis, MO 63110, USA
Pediatr Emerg Care 26:333-8. 2010..To test the hypothesis that gene expression analysis of circulating white blood cells and/or plasma cytokines could be used to improve diagnostic accuracy in children being evaluated for appendicitis... - Differences in outcome between obese and nonobese patients following severe blunt trauma are not consistent with an early inflammatory genomic responseRobert D Winfield
Department of Surgery, Laboratory of Inflammation Biology and Surgical Science, University of Florida College of Medicine, Gainesville, FL, USA
Crit Care Med 38:51-8. 2010..The effect of obesity on severely injured patients, however, remains incompletely defined. We sought to unravel potential physiologic and genomic alterations induced by obesity in severely injured blunt trauma patients... - Sequence makes a difference: paradoxical effects of stress in vivoJoseph J Wizorek
Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University, St. Louis, Missouri 63130, USA
Shock 22:229-33. 2004..The cellular mechanisms responsible for these "stressor" paradoxes in vivo are not known, but do not involve altered sepsis-induced apoptosis... - Inflammation and the host response to injury, a large-scale collaborative project: patient-oriented research core--standard operating procedures for clinical care VII--Guidelines for antibiotic administration in severely injured patientsMichael A West
Department of Surgery, University of California, San Francisco, San Francisco, California 94110, USA
J Trauma 65:1511-9. 2008..It should also be noted that not all patients with increased temperature/WBC have an infection. Discontinuation of antibiotics is appropriate if cultures and other diagnostic studies are negative... - Preliminary evidence for leukocyte transcriptional signatures for pediatric ventilator-associated pneumoniaJason A Werner
The Department of Pediatrics, St Louis University School of Medicine, St Louis, MO 63110, USA
J Intensive Care Med 27:362-9. 2012..Our hypothesis was that blood RNA expression profiles can be used to track the response to VAP in children, using the same methods that proved informational in adults... - Sepsis gene expression profiling: murine splenic compared with hepatic responses determined by using complementary DNA microarraysJ Perren Cobb
Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
Crit Care Med 30:2711-21. 2002..quot;.. - Estimating sparse gene regulatory networks using a bayesian linear regressionPinaki Sarder
Department of Electrical and Systems Engineering, Washington University, St Louis, MO 63130, USA
IEEE Trans Nanobioscience 9:121-31. 2010.... - DAP12 (KARAP) amplifies inflammation and increases mortality from endotoxemia and septic peritonitisIsaiah R Turnbull
Department of Pathology and Immunology, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110
J Exp Med 202:363-9. 2005..These data demonstrate that, during sepsis, DAP12 signaling augments the response to microbial products, amplifying inflammation and contributing to mortality... - Temporal changes in mouse aortic wall gene expression during the development of elastase-induced abdominal aortic aneurysmsSarah J Van Vickle-Chavez
Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
J Vasc Surg 43:1010-20. 2006..Knowledge of these alterations will facilitate further studies on the functional molecular mechanisms that underlie aneurysmal degeneration... - TAT-BH4 and TAT-Bcl-xL peptides protect against sepsis-induced lymphocyte apoptosis in vivoRichard S Hotchkiss
Department of Anesthesiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
J Immunol 176:5471-7. 2006..In conclusion, TAT-conjugated antiapoptotic Bcl-2-like peptides may offer a novel therapy to prevent apoptosis in sepsis and improve survival... - The surgical intensive care unit optimal mobility score predicts mortality and length of stayGeorge Kasotakis
Department of Surgery, Division of Trauma, Emergency Surgery, and Surgical Critical Care, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA
Crit Care Med 40:1122-8. 2012..To test if the surgical intensive care unit optimal mobility score predicts mortality and intensive care unit and hospital length of stay... - Global gene expression in neuroendocrine tumors from patients with the MEN1 syndromeWilliam G Dilley
Department of Surgery, Washington University School of Medicine, St, Louis, MO, USA
Mol Cancer 4:9. 2005..In vitro studies have shown menin binding to JunD, Pem, Smad3, NF-kappaB, nm23H1, and RPA2 proteins. However, none of these binding studies have led to a convincing theory of how loss-of-menin leads to neoplasia... - Surrogate consent for genomics research in intensive careAnn K Shelton
ITT Technical Institute, St Louis, Missouri, USA
Am J Crit Care 18:418-26; quiz 427. 2009..Informed consent requires explicit strategies to approach potential surrogates effectively, educate them, and ensure that informed consent has been attained... - Complement-induced regulatory T cells suppress T-cell responses but allow for dendritic-cell maturationWinfried Barchet
Washington University School of Medicine, Department of Pathology and Immunology, St Louis, MO 63110, USA
Blood 107:1497-504. 2006.... - Functional genomics of critical illness and injuryT Philip Chung
Department of Surgery, Cellular Injury and Adaptation Laboratory, Burn, Trauma, and Surgical Critical Care Section, Washington University School of Medicine, St. Louis, MO 63110-1093, USA
Crit Care Med 30:S51-7. 2002 - That which does not kill you makes you stronger: a molecular mechanism for preconditioningJonathan E McDunn
Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA
Sci STKE 2005:pe34. 2005..Recent research suggests that sequestration in stress granules of TRAF2, an adaptor protein that is required for tumor necrosis factor receptor 1 signaling, may underlie preconditioning by sublethal stresses... - The murine plasma protein response to polymicrobial intra-abdominal sepsisJonathan E McDunn
Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA Current address Department of Anesthesiology, Washington University School of Medicine, St Louis, MO 63110, USA
Proteomics Clin Appl 1:373-86. 2007..g. α-2 HS glycoprotein and zinc α-2 glycoprotein) consistent with the pathophysiology of sepsis. The panel of sepsis-associated molecular markers identified herein may prove useful in the diagnosis and categorization of sepsis... - Depletion of dendritic cells, but not macrophages, in patients with sepsisRichard S Hotchkiss
Department of Anesthesiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
J Immunol 168:2493-500. 2002..The dramatic loss of DCs in sepsis may significantly impair B and T cell function and contribute to the immune suppression that is a hallmark of the disorder... - Transcriptional profiles of human epithelial cells in response to heat: computational evidence for novel heat shock proteinsJason M Laramie
Program in Bioinformatics, Boston University, Boston, MA 02118, USA
Shock 29:623-30. 2008..Additional experiments are required to validate these putative heat shock proteins and facilitate a deeper understanding of the mechanisms involved during the stress response... - Gene expression profiles of livers from thermally injured ratsMohan R K Dasu
Shriners Hospitals for Children, 815 Market Street, Galveston, TX 77550, USA
Gene 327:51-60. 2004..The genome wide changes observed may provide a rational therapeutic strategy to improve burn care... - Microfluidic leukocyte isolation for gene expression analysis in critically ill hospitalized patientsAman Russom
Surgical Services and Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA, USA
Clin Chem 54:891-900. 2008..Here, we report the clinical validation of a novel microfluidic leukocyte nucleic acid isolation technique for gene expression analysis from critically ill, hospitalized patients that can be readily used on small volumes of blood... - Challenges and a new vision for academic surgeryJ Perren Cobb
J Am Coll Surg 200:605-6. 2005
Research Grants
- Untangling Infection from Inflammation: PneumoniaJ Cobb; Fiscal Year: 2006..Funds from this award are necessary to generate the preliminary data for a larger study, to untangle the septic response from the systemic inflammatory response. ..
- Physiological Genomics of Critical Illness and InjuryJ Cobb; Fiscal Year: 2007..Importantly, there is no other venue by which this group of investigators might meet to discuss the application of genomic technology, ethics, and human values to the study of critical illness and injury. ..