J Perren Cobb

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc Plasticity of the systemic inflammatory response to acute infection during critical illness: development of the riboleukogram
    Jonathan E McDunn
    Center for Critical Illness and Health Engineering, Department of Surgery, Washington University in St Louis, St Louis, Missouri, USA
    PLoS ONE 3:e1564. 2008
  2. ncbi request reprint Fundamentals of surgical research course: functional genomics
    J Perren Cobb
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University in St Louis, St Louis, MO 63110, USA
    J Surg Res 128:194-8. 2005
  3. ncbi request reprint The Fourth National Institutes of Health Symposium on the Functional Genomics of Critical Injury: Surviving stress from organ systems to molecules
    J Perren Cobb
    Center for Critical Illness and Health Engineering and the Department of Surgery, Washington University in St Louis, MO, USA
    Crit Care Med 36:2905-11. 2008
  4. doi request reprint Forging a critical alliance: Addressing the research needs of the United States critical illness and injury community
    J Perren Cobb
    Center for Critical Illness and Health Engineering, Washington University, St Louis, MO, USA
    Crit Care Med 37:3158-60. 2009
  5. ncbi request reprint Injury research in the genomic era
    J Perren Cobb
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University in St Louis, St Louis, Missouri 63110, USA
    Lancet 363:2076-83. 2004
  6. doi request reprint The United States critical illness and injury trials group: an introduction
    J Perren Cobb
    Center for Critical Illness and Health Engineering, Washington University in St Louis, St Louis, Missouri, USA
    J Trauma 67:S159-60. 2009
  7. pmc Validation of the riboleukogram to detect ventilator-associated pneumonia after severe injury
    J Perren Cobb
    Center for Critical Illness and Health Engineering, Washington University in St Louis, MO, USA
    Ann Surg 250:531-9. 2009
  8. ncbi request reprint Splenic CD4+ T cells have a distinct transcriptional response six hours after the onset of sepsis
    Jonathan E McDunn
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    J Am Coll Surg 203:365-75. 2006
  9. ncbi request reprint Surviving sepsis: bcl-2 overexpression modulates splenocyte transcriptional responses in vivo
    Tracey H Wagner
    Department of Anesthesiology, School of Medicine, Washington University in St Louis, St Louis, MO 63110, USA
    Am J Physiol Regul Integr Comp Physiol 292:R1751-9. 2007
  10. ncbi request reprint Commonality and differences in leukocyte gene expression patterns among three models of inflammation and injury
    Bernard H Brownstein
    Department of Genetics, Washington University, St Louis, Missouri, USA
    Physiol Genomics 24:298-309. 2006

Collaborators

Detail Information

Publications44

  1. pmc Plasticity of the systemic inflammatory response to acute infection during critical illness: development of the riboleukogram
    Jonathan E McDunn
    Center for Critical Illness and Health Engineering, Department of Surgery, Washington University in St Louis, St Louis, Missouri, USA
    PLoS ONE 3:e1564. 2008
    ..We hypothesized that circulating leukocyte transcriptional profiles can be used to monitor the host response to and recovery from infection complicating critical illness...
  2. ncbi request reprint Fundamentals of surgical research course: functional genomics
    J Perren Cobb
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University in St Louis, St Louis, MO 63110, USA
    J Surg Res 128:194-8. 2005
  3. ncbi request reprint The Fourth National Institutes of Health Symposium on the Functional Genomics of Critical Injury: Surviving stress from organ systems to molecules
    J Perren Cobb
    Center for Critical Illness and Health Engineering and the Department of Surgery, Washington University in St Louis, MO, USA
    Crit Care Med 36:2905-11. 2008
    ..Next steps for the community and suggestions for future research are presented...
  4. doi request reprint Forging a critical alliance: Addressing the research needs of the United States critical illness and injury community
    J Perren Cobb
    Center for Critical Illness and Health Engineering, Washington University, St Louis, MO, USA
    Crit Care Med 37:3158-60. 2009
    ..Discuss the research needs of the critical illness and injury communities in the United States...
  5. ncbi request reprint Injury research in the genomic era
    J Perren Cobb
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University in St Louis, St Louis, Missouri 63110, USA
    Lancet 363:2076-83. 2004
    ..It will now be important to determine how these recent technological advances can be used and integrated with our existing approaches, to reduce death, disability, and the economic consequences of trauma...
  6. doi request reprint The United States critical illness and injury trials group: an introduction
    J Perren Cobb
    Center for Critical Illness and Health Engineering, Washington University in St Louis, St Louis, Missouri, USA
    J Trauma 67:S159-60. 2009
    ....
  7. pmc Validation of the riboleukogram to detect ventilator-associated pneumonia after severe injury
    J Perren Cobb
    Center for Critical Illness and Health Engineering, Washington University in St Louis, MO, USA
    Ann Surg 250:531-9. 2009
    ..We hypothesized that circulating leukocyte RNA profiles or “riboleukograms” detect ventilator-associated pneumonia after blunt trauma...
  8. ncbi request reprint Splenic CD4+ T cells have a distinct transcriptional response six hours after the onset of sepsis
    Jonathan E McDunn
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    J Am Coll Surg 203:365-75. 2006
    ..The molecular mechanism(s) for the phenotypic changes of splenic CD4+ T cells in sepsis are not well understood...
  9. ncbi request reprint Surviving sepsis: bcl-2 overexpression modulates splenocyte transcriptional responses in vivo
    Tracey H Wagner
    Department of Anesthesiology, School of Medicine, Washington University in St Louis, St Louis, MO 63110, USA
    Am J Physiol Regul Integr Comp Physiol 292:R1751-9. 2007
    ..In addition, our data suggest that the ability of bcl-2 overexpression to improve survival in sepsis in this model is related in part to prevention of sepsis-induced alterations in spleen transcriptional responses...
  10. ncbi request reprint Commonality and differences in leukocyte gene expression patterns among three models of inflammation and injury
    Bernard H Brownstein
    Department of Genetics, Washington University, St Louis, Missouri, USA
    Physiol Genomics 24:298-309. 2006
    ..Although there is some commonality among the models, the majority of the differentially expressed genes appear to be uniquely associated with the type of injury and/or the inflammatory stimulus...
  11. ncbi request reprint A network-based analysis of systemic inflammation in humans
    Steve E Calvano
    Department of Surgery, UMDNJ Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
    Nature 437:1032-7. 2005
    ..These findings provide insight into the regulation of global leukocyte activities as they relate to innate immune system tolerance and increased susceptibility to infection in humans...
  12. pmc A genomic storm in critically injured humans
    Wenzhong Xiao
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Exp Med 208:2581-90. 2011
    ..Based on these transcriptional data, we propose a new paradigm for the human immunological response to severe injury...
  13. ncbi request reprint Molecular diagnostics in sepsis: from bedside to bench
    T Philip Chung
    Department of Surgery, School of Medicine, Washington University, St Louis, MO 63110, USA
    J Am Coll Surg 203:585-598. 2006
    ..Based on recent in vitro data, we tested the hypothesis that microarray expression profiles can be used to diagnose sepsis, distinguishing in vivo between sterile and infectious causes of systemic inflammation...
  14. pmc Clinical microfluidics for neutrophil genomics and proteomics
    Kenneth T Kotz
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 16:1042-7. 2010
    ....
  15. pmc Cell-specific expression and pathway analyses reveal alterations in trauma-related human T cell and monocyte pathways
    Krzysztof Laudanski
    Department of Surgery, University of Rochester School of Medicine, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 103:15564-9. 2006
    ..Importantly, application of cell separation, genome-wide expression, and cell-specific pathway analyses can be used to discover pathway alterations in human disease...
  16. ncbi request reprint Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice
    James A Lederer
    Department of Surgery, Brigham and Women s Hospital Harvard Medical School, Boston, Massachusetts 02115, USA
    Physiol Genomics 32:299-310. 2008
    ....
  17. pmc Streptococcus pneumoniae and Pseudomonas aeruginosa pneumonia induce distinct host responses
    Kevin W McConnell
    Departments of Surgery, Washington University School of Medicine, St Louis, MO, USA
    Crit Care Med 38:223-41. 2010
    ..The purpose of this study was to determine whether host response is similar after disparate infections with similar mortalities...
  18. ncbi request reprint Physiologic genomics
    Jonathan E McDunn
    Departments of Surgery, Genetics and Medicine, The Siteman Cancer Center, and Proteomics Center, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Surgery 139:133-9. 2006
  19. doi request reprint Using systems biology to simplify complex disease: immune cartography
    Ashoka D Polpitiya
    Department of Surgery, Washington University in St Louis, St Louis, MO, USA
    Crit Care Med 37:S16-21. 2009
    ..Large, prospective clinical trails are needed to validate the clinical utility of leukocyte RNA diagnostics (e.g., the riboleukogram)...
  20. pmc Application of genome-wide expression analysis to human health and disease
    J Perren Cobb
    Department of Surgery, Washington University, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 102:4801-6. 2005
    ....
  21. doi request reprint Myocardial transcriptional profiles in a murine model of sepsis: evidence for the importance of age
    Paul A Checchia
    Center for Critical Illness and Health Engineering and the Departments of Pediatrics and Surgery, Washington University School of Medicine, St Louis, MO, USA
    Pediatr Crit Care Med 9:530-5. 2008
    ..We hypothesized that analysis of cardiac RNA expression profiles may differentiate between the myocardial response to sepsis in young and old mice...
  22. doi request reprint Evidence for a novel blood RNA diagnostic for pediatric appendicitis: the riboleukogram
    Jared T Muenzer
    Center for Critical Illness and Health Engineering, Washington University School of Medicine, St Louis, MO 63110, USA
    Pediatr Emerg Care 26:333-8. 2010
    ..To test the hypothesis that gene expression analysis of circulating white blood cells and/or plasma cytokines could be used to improve diagnostic accuracy in children being evaluated for appendicitis...
  23. doi request reprint Differences in outcome between obese and nonobese patients following severe blunt trauma are not consistent with an early inflammatory genomic response
    Robert D Winfield
    Department of Surgery, Laboratory of Inflammation Biology and Surgical Science, University of Florida College of Medicine, Gainesville, FL, USA
    Crit Care Med 38:51-8. 2010
    ..The effect of obesity on severely injured patients, however, remains incompletely defined. We sought to unravel potential physiologic and genomic alterations induced by obesity in severely injured blunt trauma patients...
  24. ncbi request reprint Whole blood and leukocyte RNA isolation for gene expression analyses
    Robert J Feezor
    Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, USA
    Physiol Genomics 19:247-54. 2004
    ..The findings suggest that the method of RNA isolation from whole blood is a critical variable in the design of clinical studies using microarray analyses...
  25. doi request reprint Preliminary evidence for leukocyte transcriptional signatures for pediatric ventilator-associated pneumonia
    Jason A Werner
    The Department of Pediatrics, St Louis University School of Medicine, St Louis, MO 63110, USA
    J Intensive Care Med 27:362-9. 2012
    ..Our hypothesis was that blood RNA expression profiles can be used to track the response to VAP in children, using the same methods that proved informational in adults...
  26. ncbi request reprint Sequence makes a difference: paradoxical effects of stress in vivo
    Joseph J Wizorek
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University, St Louis, Missouri 63130, USA
    Shock 22:229-33. 2004
    ..The cellular mechanisms responsible for these "stressor" paradoxes in vivo are not known, but do not involve altered sepsis-induced apoptosis...
  27. doi request reprint Inflammation and the host response to injury, a large-scale collaborative project: patient-oriented research core--standard operating procedures for clinical care VII--Guidelines for antibiotic administration in severely injured patients
    Michael A West
    Department of Surgery, University of California, San Francisco, San Francisco, California 94110, USA
    J Trauma 65:1511-9. 2008
    ..It should also be noted that not all patients with increased temperature/WBC have an infection. Discontinuation of antibiotics is appropriate if cultures and other diagnostic studies are negative...
  28. ncbi request reprint Sepsis gene expression profiling: murine splenic compared with hepatic responses determined by using complementary DNA microarrays
    J Perren Cobb
    Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    Crit Care Med 30:2711-21. 2002
    ....
  29. doi request reprint Estimating sparse gene regulatory networks using a bayesian linear regression
    Pinaki Sarder
    Department of Electrical and Systems Engineering, Washington University, St Louis, MO 63130, USA
    IEEE Trans Nanobioscience 9:121-31. 2010
    ....
  30. pmc DAP12 (KARAP) amplifies inflammation and increases mortality from endotoxemia and septic peritonitis
    Isaiah R Turnbull
    Department of Pathology and Immunology, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110
    J Exp Med 202:363-9. 2005
    ..These data demonstrate that, during sepsis, DAP12 signaling augments the response to microbial products, amplifying inflammation and contributing to mortality...
  31. ncbi request reprint Temporal changes in mouse aortic wall gene expression during the development of elastase-induced abdominal aortic aneurysms
    Sarah J Van Vickle-Chavez
    Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    J Vasc Surg 43:1010-20. 2006
    ..To characterize temporal changes in mouse aortic wall gene expression associated with the development of experimental abdominal aortic aneurysms...
  32. ncbi request reprint TAT-BH4 and TAT-Bcl-xL peptides protect against sepsis-induced lymphocyte apoptosis in vivo
    Richard S Hotchkiss
    Department of Anesthesiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Immunol 176:5471-7. 2006
    ..In conclusion, TAT-conjugated antiapoptotic Bcl-2-like peptides may offer a novel therapy to prevent apoptosis in sepsis and improve survival...
  33. ncbi request reprint That which does not kill you makes you stronger: a molecular mechanism for preconditioning
    Jonathan E McDunn
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    Sci STKE 2005:pe34. 2005
    ..Recent research suggests that sequestration in stress granules of TRAF2, an adaptor protein that is required for tumor necrosis factor receptor 1 signaling, may underlie preconditioning by sublethal stresses...
  34. doi request reprint The surgical intensive care unit optimal mobility score predicts mortality and length of stay
    George Kasotakis
    Department of Surgery, Division of Trauma, Emergency Surgery, and Surgical Critical Care, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA
    Crit Care Med 40:1122-8. 2012
    ..To test if the surgical intensive care unit optimal mobility score predicts mortality and intensive care unit and hospital length of stay...
  35. pmc Global gene expression in neuroendocrine tumors from patients with the MEN1 syndrome
    William G Dilley
    Department of Surgery, Washington University School of Medicine, St, Louis, MO, USA
    Mol Cancer 4:9. 2005
    ..In vitro studies have shown menin binding to JunD, Pem, Smad3, NF-kappaB, nm23H1, and RPA2 proteins. However, none of these binding studies have led to a convincing theory of how loss-of-menin leads to neoplasia...
  36. pmc Complement-induced regulatory T cells suppress T-cell responses but allow for dendritic-cell maturation
    Winfried Barchet
    Washington University School of Medicine, Department of Pathology and Immunology, St Louis, MO 63110, USA
    Blood 107:1497-504. 2006
    ....
  37. ncbi request reprint Functional genomics of critical illness and injury
    T Philip Chung
    Department of Surgery, Cellular Injury and Adaptation Laboratory, Burn, Trauma, and Surgical Critical Care Section, Washington University School of Medicine, St Louis, MO 63110 1093, USA
    Crit Care Med 30:S51-7. 2002
  38. doi request reprint Surrogate consent for genomics research in intensive care
    Ann K Shelton
    ITT Technical Institute, St Louis, Missouri, USA
    Am J Crit Care 18:418-26; quiz 427. 2009
    ..Informed consent requires explicit strategies to approach potential surrogates effectively, educate them, and ensure that informed consent has been attained...
  39. doi request reprint The murine plasma protein response to polymicrobial intra-abdominal sepsis
    Jonathan E McDunn
    Cellular Injury and Adaptation Laboratory, Department of Surgery, Washington University School of Medicine, St Louis, MO, USA Current address Department of Anesthesiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proteomics Clin Appl 1:373-86. 2007
    ..g. α-2 HS glycoprotein and zinc α-2 glycoprotein) consistent with the pathophysiology of sepsis. The panel of sepsis-associated molecular markers identified herein may prove useful in the diagnosis and categorization of sepsis...
  40. ncbi request reprint Depletion of dendritic cells, but not macrophages, in patients with sepsis
    Richard S Hotchkiss
    Department of Anesthesiology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Immunol 168:2493-500. 2002
    ..The dramatic loss of DCs in sepsis may significantly impair B and T cell function and contribute to the immune suppression that is a hallmark of the disorder...
  41. ncbi request reprint Transcriptional profiles of human epithelial cells in response to heat: computational evidence for novel heat shock proteins
    Jason M Laramie
    Program in Bioinformatics, Boston University, Boston, MA 02118, USA
    Shock 29:623-30. 2008
    ..Additional experiments are required to validate these putative heat shock proteins and facilitate a deeper understanding of the mechanisms involved during the stress response...
  42. ncbi request reprint Gene expression profiles of livers from thermally injured rats
    Mohan R K Dasu
    Shriners Hospitals for Children, 815 Market Street, Galveston, TX 77550, USA
    Gene 327:51-60. 2004
    ..The genome wide changes observed may provide a rational therapeutic strategy to improve burn care...
  43. doi request reprint Microfluidic leukocyte isolation for gene expression analysis in critically ill hospitalized patients
    Aman Russom
    Surgical Services and Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA, USA
    Clin Chem 54:891-900. 2008
    ..Here, we report the clinical validation of a novel microfluidic leukocyte nucleic acid isolation technique for gene expression analysis from critically ill, hospitalized patients that can be readily used on small volumes of blood...
  44. ncbi request reprint Challenges and a new vision for academic surgery
    J Perren Cobb
    J Am Coll Surg 200:605-6. 2005

Research Grants5

  1. Untangling Infection from Inflammation: Pneumonia
    J Cobb; Fiscal Year: 2006
    ..Funds from this award are necessary to generate the preliminary data for a larger study, to untangle the septic response from the systemic inflammatory response. ..
  2. Physiological Genomics of Critical Illness and Injury
    J Cobb; Fiscal Year: 2007
    ..Importantly, there is no other venue by which this group of investigators might meet to discuss the application of genomic technology, ethics, and human values to the study of critical illness and injury. ..