PETER BURGERS

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. ncbi request reprint Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase delta
    P M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 273:19756-62. 1998
  2. pmc A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis
    Alena V Makarova
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Nucleic Acids Res 40:11618-26. 2012
  3. pmc It's all about flaps: Dna2 and checkpoint activation
    Peter M Burgers
    Washington University School of Medicine St Louis, MO USA
    Cell Cycle 10:2423-2. 2011
  4. ncbi request reprint Eukaryotic DNA polymerases: proposal for a revised nomenclature
    P M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:43487-90. 2001
  5. pmc Polymerase dynamics at the eukaryotic DNA replication fork
    Peter M J Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:4041-5. 2009
  6. pmc Yeast exonuclease 5 is essential for mitochondrial genome maintenance
    Peter M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell Biol 30:1457-66. 2010
  7. ncbi request reprint Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta
    K J Gerik
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 273:19747-55. 1998
  8. ncbi request reprint Structure of DNA polymerase delta from Saccharomyces cerevisiae
    E Johansson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:43824-8. 2001
  9. ncbi request reprint Eukaryotic DNA polymerases in DNA replication and DNA repair
    P M Burgers
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, St Louis, MO 63110, USA
    Chromosoma 107:218-27. 1998
  10. ncbi request reprint Saccharomyces cerevisiae replication factor C. I. Purification and characterization of its ATPase activity
    B L Yoder
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 266:22689-97. 1991

Research Grants

Collaborators

  • Michael A Resnick
  • Hong Wang
  • Patricia L Opresko
  • A Sugino
  • Z Wang
  • T Todo
  • E C Friedberg
  • C W Lawrence
  • R Woodgate
  • E V Koonin
  • Wang Zhiping
  • J L Li
  • Arthur Kornberg
  • T A Kunkel
  • X V Gomes
  • Parie Garg
  • S L Schmidt
  • Justin D Lormand
  • Alena V Makarova
  • Olga Chilkova
  • X Li
  • Erik Johansson
  • Carrie M Stith
  • Yong Hwan Jin
  • Alexa A Franco
  • K J Gerik
  • Jerzy Majka
  • Leonid Dzantiev
  • Marietta Y Lee
  • Parminder Kaur
  • Connor T Murphy
  • Noah Buncher
  • Joseph L Stodola
  • E Johansson
  • Isabelle Isoz
  • Pawel Grabowski
  • Else Britt Lundström
  • Peter Stenlund
  • Hanan Al-Refai
  • Carrie M W Stith
  • Joan F Sterling
  • Wendy M Lam
  • Dmitry A Gordenin
  • Laura J W Murray
  • Paul D Kaufman
  • Ravi R Iyer
  • Jochen Genschel
  • Nasim Sabouri
  • Nicoleta Constantin
  • Paul Modrich
  • S L Gary
  • A L Pautz
  • J Majka
  • R Ayyagari
  • A Pautz
  • K J Impellizzeri
  • B L Yoder
  • J Harrington
  • M R Lieber
  • G A Bauer
  • K J Percival
  • M B Klein
  • B Anderson

Detail Information

Publications32

  1. ncbi request reprint Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase delta
    P M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 273:19756-62. 1998
    ..However, Poldelta*-mediated DNA synthesis proceeded inefficiently and was characterized by frequent pause sites. Reconstitution of Poldelta was achieved upon addition of Pol32p to Poldelta*...
  2. pmc A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis
    Alena V Makarova
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Nucleic Acids Res 40:11618-26. 2012
    ..A stable Pol ζ(4) complex can be identified in all phases of the cell cycle suggesting that this complex is not regulated at the level of protein interactions between Rev3-Rev7 and Pol31-Pol32...
  3. pmc It's all about flaps: Dna2 and checkpoint activation
    Peter M Burgers
    Washington University School of Medicine St Louis, MO USA
    Cell Cycle 10:2423-2. 2011
    ..Comment on: Budd ME, et al. Cell Cycle 2011; 10:1690-8...
  4. ncbi request reprint Eukaryotic DNA polymerases: proposal for a revised nomenclature
    P M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:43487-90. 2001
  5. pmc Polymerase dynamics at the eukaryotic DNA replication fork
    Peter M J Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:4041-5. 2009
    ..In the more common short flap pathway, Pol delta coordinates with the flap endonuclease FEN1 to degrade initiator RNA, whereas in the long flap pathway, RNA removal is initiated by the Dna2 nuclease/helicase...
  6. pmc Yeast exonuclease 5 is essential for mitochondrial genome maintenance
    Peter M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell Biol 30:1457-66. 2010
    ..However, Exo5 has the capacity to slide across 5' double-stranded DNA or 5' RNA sequences and resumes cutting two nucleotides downstream of the double-stranded-to-single-stranded junction or RNA-to-DNA junction, respectively...
  7. ncbi request reprint Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta
    K J Gerik
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 273:19747-55. 1998
    ..When POL31 and POL32 were co-expressed in Escherichia coli, a tetrameric (Pol31p.Pol32p)2 species was detected by gel filtration, indicating that the two subunits form a complex...
  8. ncbi request reprint Structure of DNA polymerase delta from Saccharomyces cerevisiae
    E Johansson
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:43824-8. 2001
    ..Moreover, a two-hybrid analysis of the Pol32 subunit did not detect a Pol32-Pol32 interaction in vivo. Therefore, we conclude that the assembly structure of Pol delta is that of a monomer...
  9. ncbi request reprint Eukaryotic DNA polymerases in DNA replication and DNA repair
    P M Burgers
    Washington University School of Medicine, Department of Biochemistry and Molecular Biophysics, St Louis, MO 63110, USA
    Chromosoma 107:218-27. 1998
    ..The role of DNA polymerase beta in base-excision repair is well established for mammalian systems, but in yeast, DNA polymerase delta appears to fulfill that function...
  10. ncbi request reprint Saccharomyces cerevisiae replication factor C. I. Purification and characterization of its ATPase activity
    B L Yoder
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 266:22689-97. 1991
    ..These results attest to the structural and functional homology between yeast and mammalian cells for these components of the replication machinery...
  11. pmc A mutational analysis of the yeast proliferating cell nuclear antigen indicates distinct roles in DNA replication and DNA repair
    R Ayyagari
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Cell Biol 15:4420-9. 1995
    ..Therefore, DNA repair requires interactions between repair-specific protein(s) and PCNA, which are distinct from those required for DNA replication...
  12. ncbi request reprint Lagging strand DNA synthesis at the eukaryotic replication fork involves binding and stimulation of FEN-1 by proliferating cell nuclear antigen
    X Li
    Department of Biochemistry, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 270:22109-12. 1995
    ..This interaction is important in the physical orchestration of lagging strand synthesis and may have implications for how PCNA stimulates other members of the FEN-1 nuclease family in a broad range of DNA metabolic transactions...
  13. pmc Two modes of FEN1 binding to PCNA regulated by DNA
    X V Gomes
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    EMBO J 19:3811-21. 2000
    ..An FF-->GA mutation in the PCNA-interaction domain of FEN1 severely decreased both modes of interaction with PCNA and resulted in replication and repair defects in vivo...
  14. pmc The yeast analog of mammalian cyclin/proliferating-cell nuclear antigen interacts with mammalian DNA polymerase delta
    G A Bauer
    Department of Biological Chemistry, Washington University School of Medicine, Saint Louis, MO 63110
    Proc Natl Acad Sci U S A 85:7506-10. 1988
    ..Yeast DNA polymerases I and II and calf thymus DNA polymerase alpha are not stimulated by yPCNA. Treatment of logarithmic-phase cells with hydroxyurea blocks them in the S phase and produces a 4- to 5-fold increase in yPCNA...
  15. pmc Molecular cloning and expression of the Saccharomyces cerevisiae RFC3 gene, an essential component of replication factor C
    X Li
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110
    Proc Natl Acad Sci U S A 91:868-72. 1994
    ..coli and purified to homogeneity. Purified Rfc3p has an ATPase activity that is markedly stimulated by single-stranded DNA but not by double-stranded DNA or RNA...
  16. ncbi request reprint Cloning and characterization of the essential Saccharomyces cerevisiae RFC4 gene encoding the 37-kDa subunit of replication factor C
    X Li
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 269:21880-4. 1994
    ..However, Rfc4p formed a tight complex with the product of the RFC3 gene which encodes the ATPase of RF-C...
  17. ncbi request reprint Overproduction and affinity purification of Saccharomyces cerevisiae replication factor C
    K J Gerik
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 272:1256-62. 1997
    ..RF-C from the overproduction strain purified by this procedure was essentially homogeneous and had a severalfold higher specific activity than RF-C preparations that had previously been purified through multicolumn procedures...
  18. pmc DNA polymerase δ stalls on telomeric lagging strand templates independently from G-quadruplex formation
    Justin D Lormand
    Department of Environmental and Occupational Health, University of Pittsburgh Graduate School of Public Health, 100 Technology Drive, Pittsburgh, PA 15219, USA, Department of Physics, North Carolina State University, 2401 Stinson Drive, Raleigh, NC, 27695, USA, Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA, Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA and Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Nucleic Acids Res 41:10323-33. 2013
    ..Our data indicate that G4 formation is not required for polymerase stalling on telomeric lagging strands and suggest that an alternative mechanism, in addition to stable G4s, contributes to replication stalling at telomeres. ..
  19. ncbi request reprint Molecular cloning and primary structure of the uracil-DNA-glycosylase gene from Saccharomyces cerevisiae
    K J Percival
    Department of Biological Chemistry, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 264:2593-8. 1989
    ..Genetic mapping experiments have localized the UNG1 gene on the left arm of chromosome XIII at 17 cM from the GAL80 locus proximal to the centromer. Deletions of the UNG1 gene are viable...
  20. pmc The spectrum of spontaneous mutations in a Saccharomyces cerevisiae uracil-DNA-glycosylase mutant limits the function of this enzyme to cytosine deamination repair
    K J Impellizzeri
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110
    J Bacteriol 173:6807-10. 1991
    ..cerevisiae and that the UNG1 gene is not required for strand-specific mismatch repair in S. cerevisiae...
  21. ncbi request reprint ATP utilization by yeast replication factor C. I. ATP-mediated interaction with DNA and with proliferating cell nuclear antigen
    X V Gomes
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Biol Chem 276:34768-75. 2001
    ..Filter binding experiments and analysis of proteins bound to DNA-magnetic beads confirmed the conclusions drawn from the surface plasmon resonance studies...
  22. ncbi request reprint ATP utilization by yeast replication factor C. III. The ATP-binding domains of Rfc2, Rfc3, and Rfc4 are essential for DNA recognition and clamp loading
    S L Schmidt
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:34784-91. 2001
    ....
  23. ncbi request reprint ATP utilization by yeast replication factor C. IV. RFC ATP-binding mutants show defects in DNA replication, DNA repair, and checkpoint regulation
    S L Schmidt
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:34792-800. 2001
    ..These data demonstrate that the ATP binding function of RFC2 is important for both DNA replication and checkpoint function and, for the first time, that RFC4 also plays a role in checkpoint regulation...
  24. ncbi request reprint ATP utilization by yeast replication factor C. II. Multiple stepwise ATP binding events are required to load proliferating cell nuclear antigen onto primed DNA
    X V Gomes
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Biol Chem 276:34776-83. 2001
    ..RFC.ATP(3); (iv) a conformational change in the latter complex reveals a fourth binding site for ATP; and (v) the DNA.PCNA.RFC.ATP(4) complex is finally competent for completion of PCNA loading and release of RFC upon hydrolysis of ATP...
  25. pmc The eukaryotic leading and lagging strand DNA polymerases are loaded onto primer-ends via separate mechanisms but have comparable processivity in the presence of PCNA
    Olga Chilkova
    Department of Medical Biochemistry and Biophysics, Department of Biochemistry, Umea University, 901 87 Umea, Sweden
    Nucleic Acids Res 35:6588-97. 2007
    ..We conclude that Pol epsilon and Pol delta exhibit comparable processivity, but are loaded on the primer-end via different mechanisms...
  26. ncbi request reprint Arthur Kornberg (1918-2007)
    Peter M Burgers
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell 28:530-2. 2007
  27. pmc The multiple biological roles of the 3'-->5' exonuclease of Saccharomyces cerevisiae DNA polymerase delta require switching between the polymerase and exonuclease domains
    Yong Hwan Jin
    National Institute of Environmental Health Sciences, D3 01, 101 TW Alexander Dr, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mol Cell Biol 25:461-71. 2005
    ..We conclude that the three biological functions of the 3'-->5' exonuclease addressed in this study are performed intramolecularly within the replicating holoenzyme...
  28. pmc Idling by DNA polymerase delta maintains a ligatable nick during lagging-strand DNA replication
    Parie Garg
    Department of Biochemistry, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Genes Dev 18:2764-73. 2004
    ..Consistent with the hypothesis that DNA polymerase epsilon is the leading-strand enzyme, we observed no idling by this enzyme and no cooperation with FEN1 for creating a ligatable nick...
  29. pmc Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1
    Parie Garg
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 102:18361-6. 2005
    ..We propose that ubiquitination of PCNA increases its functionality as a sliding clamp to promote mutagenic DNA replication...
  30. ncbi request reprint Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint
    Jerzy Majka
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    DNA Repair (Amst) 4:1189-94. 2005
    ..In agreement, overexpression of DDC1 or RAD17 in a MEC3Delta strain, or of MEC3 or RAD17 in a DDC1Delta strain shows no rescue of damage sensitivity...
  31. pmc Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C
    Alexa A Franco
    Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    Genes Dev 19:1365-75. 2005
    ..We conclude that histone chaperone protein Asf1 maintains a subset of replication elongation factors at stalled replication forks and directly interacts with the replication machinery...
  32. ncbi request reprint A defined human system that supports bidirectional mismatch-provoked excision
    Leonid Dzantiev
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Mol Cell 15:31-41. 2004
    ..By contrast, RFC and PCNA have only a limited effect on 5' to 3' excision directed by a 5' strand break...

Research Grants31

  1. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2009
    ..These pathways are conserved from human to yeast, and we are proposing to study these pathways in yeast, because this model organism is more approachable to genetic and biochemical analysis. ..
  2. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    Peter M Burgers; Fiscal Year: 2010
    ..These pathways are conserved from human to yeast, and we are proposing to study these pathways in yeast, because this model organism is more approachable to genetic and biochemical analysis. ..
  3. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 1999
    ..Improper regulation of these processes in humans may lead to cancer. ..
  4. STRUCTURE/FUNCTION OF YEAST DNA POLYMERASE DELTA
    PETER BURGERS; Fiscal Year: 2000
    ..Improper regulation of these processes in humans may lead to cancer. ..
  5. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2001
    ..Improper function and regulation of these processes in humans may lead to the accumulation of mutations and cancer. ..
  6. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2003
    ..Improper function and regulation of these processes in humans may lead to the accumulation of mutations and cancer. ..
  7. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2004
    ..Improper function and regulation of these processes in humans may lead to the accumulation of mutations and cancer. ..
  8. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2005
    ..Improper function and regulation of these processes in humans may lead to the accumulation of mutations and chromosome abnormalities, and in cancer. ..
  9. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2007
    ..Improper function and regulation of these processes in humans may lead to the accumulation of mutations and chromosome abnormalities, and in cancer. ..
  10. KINASE ACTIVATION IN THE DNA DAMAGE CHECKPOINTS
    PETER BURGERS; Fiscal Year: 2009
    ..Checkpoint pathways are conserved from human to yeast, and we are proposing to study these pathways in yeast, because as a model organism it is more approachable to genetic and biochemical analysis. ..
  11. KINASE ACTIVATION IN THE DNA DAMAGE CHECKPOINTS
    Peter M Burgers; Fiscal Year: 2010
    ..Checkpoint pathways are conserved from human to yeast, and we are proposing to study these pathways in yeast, because as a model organism it is more approachable to genetic and biochemical analysis. ..
  12. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 1993
    ..Thus, an intensive study of the mechanism of DNA replication and its regulation in yeast will aid in understanding the mechanisms that induce or prevent mutations as well as those that control cell division in human cells...
  13. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 1991
    ..In addition, a better understanding of the mechanisms that induce or prevent mutations in yeast may lead to a better understanding of similar mechanisms in humans...
  14. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 1992
    ..Thus, an intensive study of the mechanism of DNA replication and its regulation in yeast will aid in understanding the mechanisms that induce or prevent mutations as well as those that control cell division in human cells...
  15. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2002
    ..Improper function and regulation of these processes in humans may lead to the accumulation of mutations and cancer. ..
  16. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 1990
    ..In addition, a better understanding of the mechanisms that induce or prevent mutations in yeast may lead to a better understanding of similar mechanisms in humans...
  17. STRUCTURE/FUNCTION OF YEAST DNA POLYMERASE DELTA
    PETER BURGERS; Fiscal Year: 1999
    ..Improper regulation of these processes in humans may lead to cancer. ..
  18. ENZYMOLOGY OF REPLICATION OF YEAST CHROMOSOMAL DNA
    PETER BURGERS; Fiscal Year: 2006
    ..Improper function and regulation of these processes in humans may lead to the accumulation of mutations and chromosome abnormalities, and in cancer. ..
  19. STRUCTURE/FUNCTION OF YEAST DNA POLYMERASE DELTA
    PETER BURGERS; Fiscal Year: 2001
    ..Improper regulation of these processes in humans may lead to cancer. ..
  20. STRUCTURE/FUNCTION OF YEAST DNA POLYMERASE DELTA
    PETER BURGERS; Fiscal Year: 2002
    ..Improper regulation of these processes in humans may lead to cancer. ..