Guojun Bu

Summary

Affiliation: Washington University School of Medicine
Country: USA

Publications

  1. pmc Low-density lipoprotein receptor-related protein 1 (LRP1) mediates neuronal Abeta42 uptake and lysosomal trafficking
    Rodrigo A Fuentealba
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 5:e11884. 2010
  2. pmc Wnt signaling activation and mammary gland hyperplasia in MMTV-LRP6 transgenic mice: implication for breast cancer tumorigenesis
    J Zhang
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Oncogene 29:539-49. 2010
  3. pmc Recent advances in basic neurosciences and brain disease: from synapses to behavior
    Guo Qiang Bi
    Department of Neurobiology, University of Pittsburgh, Pittsburgh, USA
    Mol Pain 2:38. 2006
  4. ncbi request reprint LRP in amyloid-beta production and metabolism
    Guojun Bu
    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, CB 8208, St Louis, MO 63110, USA
    Ann N Y Acad Sci 1086:35-53. 2006
  5. pmc Apolipoprotein E and its receptors in Alzheimer's disease: pathways, pathogenesis and therapy
    Guojun Bu
    Hope Center for Neurological Disorders, Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nat Rev Neurosci 10:333-44. 2009
  6. pmc Receptor-associated protein interacts with amyloid-beta peptide and promotes its cellular uptake
    Takahisa Kanekiyo
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:33352-9. 2009
  7. ncbi request reprint Apolipoprotein E and low density lipoprotein receptor-related protein facilitate intraneuronal Abeta42 accumulation in amyloid model mice
    Celina V Zerbinatti
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:36180-6. 2006
  8. pmc Identification of acidic dileucine signals in LRP9 that interact with both GGAs and AP-1/AP-2
    Balraj Doray
    Department of Internal Medicine, Washington University School of Medicine in St Louis, St Louis, MO 63110, USA
    Traffic 9:1551-62. 2008
  9. pmc Heparan sulphate proteoglycan and the low-density lipoprotein receptor-related protein 1 constitute major pathways for neuronal amyloid-beta uptake
    Takahisa Kanekiyo
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 31:1644-51. 2011
  10. pmc Adaptor protein sorting nexin 17 regulates amyloid precursor protein trafficking and processing in the early endosomes
    Jiyeon Lee
    Department of Pediatrics and Cell Biology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:11501-8. 2008

Collaborators

Detail Information

Publications73

  1. pmc Low-density lipoprotein receptor-related protein 1 (LRP1) mediates neuronal Abeta42 uptake and lysosomal trafficking
    Rodrigo A Fuentealba
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 5:e11884. 2010
    ..However, direct evidence of neuronal endocytosis of Abeta42 through LRP1 is lacking...
  2. pmc Wnt signaling activation and mammary gland hyperplasia in MMTV-LRP6 transgenic mice: implication for breast cancer tumorigenesis
    J Zhang
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Oncogene 29:539-49. 2010
    ..Our results suggest that Wnt signaling activation at the cell-surface receptor level can contribute to breast cancer tumorigenesis...
  3. pmc Recent advances in basic neurosciences and brain disease: from synapses to behavior
    Guo Qiang Bi
    Department of Neurobiology, University of Pittsburgh, Pittsburgh, USA
    Mol Pain 2:38. 2006
    ..The second international meeting on Neurons and Brain Disease will be held in Toronto (August 29-31, 2007)...
  4. ncbi request reprint LRP in amyloid-beta production and metabolism
    Guojun Bu
    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, CB 8208, St Louis, MO 63110, USA
    Ann N Y Acad Sci 1086:35-53. 2006
    ..Understanding the pathways that modulate brain Abeta metabolism may enable the rational design of molecular medicine to treat AD...
  5. pmc Apolipoprotein E and its receptors in Alzheimer's disease: pathways, pathogenesis and therapy
    Guojun Bu
    Hope Center for Neurological Disorders, Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nat Rev Neurosci 10:333-44. 2009
    ..Emerging knowledge of the contribution of APOE to the pathophysiology of AD presents new opportunities for AD therapy...
  6. pmc Receptor-associated protein interacts with amyloid-beta peptide and promotes its cellular uptake
    Takahisa Kanekiyo
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:33352-9. 2009
    ..Our findings reveal that RAP is a novel Abeta-binding protein that promotes cellular internalization of Abeta...
  7. ncbi request reprint Apolipoprotein E and low density lipoprotein receptor-related protein facilitate intraneuronal Abeta42 accumulation in amyloid model mice
    Celina V Zerbinatti
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:36180-6. 2006
    ..Our results support the hypothesis that LRP binds and endocytoses Abeta42 both directly and via apoE but that endocytosed Abeta42 is not completely degraded and accumulates in intraneuronal lysosomes...
  8. pmc Identification of acidic dileucine signals in LRP9 that interact with both GGAs and AP-1/AP-2
    Balraj Doray
    Department of Internal Medicine, Washington University School of Medicine in St Louis, St Louis, MO 63110, USA
    Traffic 9:1551-62. 2008
    ..Either AC-LL motif of LRP9 is functional in endocytosis. These findings represent the first study characterizing the trafficking of LRP9 and also have implications for the identification of additional GGA cargo molecules...
  9. pmc Heparan sulphate proteoglycan and the low-density lipoprotein receptor-related protein 1 constitute major pathways for neuronal amyloid-beta uptake
    Takahisa Kanekiyo
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 31:1644-51. 2011
    ..Our findings demonstrate that LRP1 and HSPG function in a cooperative manner to mediate cellular Aβ uptake and define a major pathway through which Aβ gains entry to neuronal cells...
  10. pmc Adaptor protein sorting nexin 17 regulates amyloid precursor protein trafficking and processing in the early endosomes
    Jiyeon Lee
    Department of Pediatrics and Cell Biology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:11501-8. 2008
    ..The identification of SNX17 as a novel APP intracellular adaptor protein highly expressed in neurons should facilitate the understanding of the relationship between APP intracellular trafficking and processing to Abeta...
  11. ncbi request reprint Rapid endocytosis of the low density lipoprotein receptor-related protein modulates cell surface distribution and processing of the beta-amyloid precursor protein
    Judy A Cam
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:15464-70. 2005
    ..Together, these data establish a relationship between LRP rapid endocytosis and APP trafficking and proteolytic processing to generate Abeta...
  12. pmc Sorting nexin 17 facilitates LRP recycling in the early endosome
    Peter van Kerkhof
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    EMBO J 24:2851-61. 2005
    ..We conclude that Snx 17 binds to a motif in the LRP tail distinct from the endocytosis signals and promotes LRP sorting to the recycling pathway in the early endosomes...
  13. pmc Amyloid precursor protein regulates brain apolipoprotein E and cholesterol metabolism through lipoprotein receptor LRP1
    Qiang Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Neuron 56:66-78. 2007
    ....
  14. pmc Low-density lipoprotein receptor-related protein 1 promotes cancer cell migration and invasion by inducing the expression of matrix metalloproteinases 2 and 9
    Heesang Song
    Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cancer Res 69:879-86. 2009
    ..Together, our data strongly suggest that LRP1 promotes glioblastoma cell migration and invasion by regulating the expression and function of MMP2 and MMP9 perhaps via an ERK-dependent signaling pathway...
  15. pmc LRP6 overexpression defines a class of breast cancer subtype and is a target for therapy
    Chia Chen Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 107:5136-41. 2010
    ..Together, our studies highlight LRP6 as a potential therapeutic target in breast cancer, and introduce Mesd as a promising antitumor agent for treating breast cancer subtypes with Wnt activation at the cell surface...
  16. pmc ApoE influences amyloid-β (Aβ) clearance despite minimal apoE/Aβ association in physiological conditions
    Philip B Verghese
    Department of Neurology, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 110:E1807-16. 2013
    ..These results provide an alternative frame work for the mechanistic explanations on how apoE isoforms influence the risk of AD pathogenesis...
  17. ncbi request reprint Mesd binds to mature LDL-receptor-related protein-6 and antagonizes ligand binding
    Yonghe Li
    Department of Pediatrics, St Louis Children s Hospital, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    J Cell Sci 118:5305-14. 2005
    ..Together our results show that in addition to serving as a folding chaperone, Mesd can function as a receptor antagonist by inhibiting ligand binding to mature LRP6...
  18. ncbi request reprint The low density lipoprotein receptor regulates the level of central nervous system human and murine apolipoprotein E but does not modify amyloid plaque pathology in PDAPP mice
    John D Fryer
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:25754-9. 2005
    ....
  19. pmc Low density lipoprotein receptor-related protein 1 promotes anti-apoptotic signaling in neurons by activating Akt survival pathway
    Rodrigo A Fuentealba
    Department of Pediatrics, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:34045-53. 2009
    ....
  20. pmc Slow endocytosis of the LDL receptor-related protein 1B: implications for a novel cytoplasmic tail conformation
    Jane M Knisely
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Exp Cell Res 313:3298-307. 2007
    ..From these data, we propose that both halves of the LRP1B cytoplasmic tail contribute to a unique global conformation, which results in less efficient recognition by endocytic adaptors and a slow endocytosis rate...
  21. pmc Neuronal clearance of amyloid-β by endocytic receptor LRP1
    Takahisa Kanekiyo
    Department of Neuroscience, Mayo Clinic, Jacksonville, Florida 32224, Department of Neurology, Knight Alzheimer s Disease Research Center, and Hope Center for Neurological Disorders, Washington University, St Louis, Missouri 63110, and Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, College of Medicine, Xiamen University, Xiamen 361005, China
    J Neurosci 33:19276-83. 2013
    ..Together, our results demonstrate that LRP1 plays an important role in receptor-mediated clearance of Aβ and indicate that neurons not only produce but also clear Aβ. ..
  22. pmc Amyloid seeds formed by cellular uptake, concentration, and aggregation of the amyloid-beta peptide
    Xiaoyan Hu
    Hope Center for Neurological Disorders and Department of Neurology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 106:20324-9. 2009
    ..We speculate that extrusion of these aggregates may seed extracellular amyloid plaque formation during AD pathogenesis...
  23. pmc Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains
    Chia Chen Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 284:15299-307. 2009
    ..Our results also support the development of soluble LRP6 receptors and Mesd as potential therapeutic molecules that target Wnt signaling...
  24. ncbi request reprint Independent and cooperative roles of N-glycans and molecular chaperones in the folding and disulfide bond formation of the low-density lipoprotein (LDL) receptor-related protein
    Lynn M McCormick
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 44:5794-803. 2005
    ....
  25. ncbi request reprint Degradation of the LDL receptor class 2 mutants is mediated by a proteasome-dependent pathway
    Yonghe Li
    Department of Pediatrics, St Louis Children s Hospital, Washington University School of Medicine, St Louis, MO 63110, USA
    J Lipid Res 45:1084-91. 2004
    ..Taken together, these data demonstrate that the proteasome is the principal degradation pathway for LDLR class 2 mutants...
  26. pmc Neuronal LRP1 knockout in adult mice leads to impaired brain lipid metabolism and progressive, age-dependent synapse loss and neurodegeneration
    Qiang Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 30:17068-78. 2010
    ..Together, these studies support a critical role for LRP1 in maintaining brain lipid homeostasis and associated synaptic and neuronal integrity, and provide important insights into the pathophysiological mechanisms in AD...
  27. ncbi request reprint The low density lipoprotein receptor-related protein 1B retains beta-amyloid precursor protein at the cell surface and reduces amyloid-beta peptide production
    Judy A Cam
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:29639-46. 2004
    ..These findings reveal that LRP1B is a novel binding partner of APP that functions to decrease APP processing to Abeta. Consequently LRP1B expression could function to protect against the pathogenesis of Alzheimer's disease...
  28. ncbi request reprint LRP6 expression promotes cancer cell proliferation and tumorigenesis by altering beta-catenin subcellular distribution
    Yonghe Li
    Department of Pediatrics, St Louis Children s Hospital, Washington University School of Medicine, St Louis, MO 63110, USA
    Oncogene 23:9129-35. 2004
    ..Finally, we demonstrate that LRP6 expression promotes tumorigenesis in vivo. These results thus indicate that LRP6 may function as a potential oncogenic protein by modulating Wnt/beta-catenin signaling...
  29. ncbi request reprint Modulation of LRP6-mediated Wnt signaling by molecular chaperone Mesd
    Yonghe Li
    Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, MO 63110, USA
    FEBS Lett 580:5423-8. 2006
    ..Our results present novel mechanisms by which Mesd and LRP6 modulate Wnt signaling...
  30. pmc Endocytosis is required for synaptic activity-dependent release of amyloid-beta in vivo
    John R Cirrito
    Department of Neurology, Washington University School of Medicine, St Louis, MO 63110, USA
    Neuron 58:42-51. 2008
    ..These findings have implications for AD pathogenesis and may provide insights into therapeutic intervention...
  31. ncbi request reprint Measurement of receptor endocytosis and recycling
    Jane M Knisely
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO, USA
    Methods Mol Biol 457:319-32. 2008
    ..Herein, the authors describe quantitative methods to measure the endocytosis and recycling capacity of receptors, using LRP as a model receptor...
  32. pmc Breast cancer-derived Dickkopf1 inhibits osteoblast differentiation and osteoprotegerin expression: implication for breast cancer osteolytic bone metastases
    Guojun Bu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO, USA
    Int J Cancer 123:1034-42. 2008
    ..Taken together, these results suggest that breast cancer-produced Dkk1 may be an important mechanistic link between primary breast tumors and secondary osteolytic bone metastases...
  33. ncbi request reprint Striking differences of LDL receptor-related protein 1B expression in mouse and human
    Yonghe Li
    Department of Pediatrics, Washington University School of Medicine, St Louis Children s Hospital, St Louis, MO 63110, USA
    Biochem Biophys Res Commun 333:868-73. 2005
    ..The broader expression pattern of LRP1B in human tissues suggests that this putative tumor suppressor may play roles in several types of human cancer...
  34. ncbi request reprint Estrogen therapy fails to alter amyloid deposition in the PDAPP model of Alzheimer's disease
    Pattie S Green
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Endocrinology 146:2774-81. 2005
    ..Our observations indicate that long-term estrogen therapy does not alter amyloid pathology in PDAPP mice, an animal model of Alzheimer's disease, and question the role of estrogen in Abeta deposition in brain...
  35. pmc Apolipoprotein E and apolipoprotein E receptors: normal biology and roles in Alzheimer disease
    David M Holtzman
    Department of Neurology, Alzheimer s Disease Research Center, Hope Center for Neurological Disorders, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Cold Spring Harb Perspect Med 2:a006312. 2012
    ..Understanding the normal and disease-related biology connecting apoE, apoE receptors, and AD is likely to provide novel insights into AD pathogenesis and treatment...
  36. pmc Lipoprotein receptor LRP1 regulates leptin signaling and energy homeostasis in the adult central nervous system
    Qiang Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Biol 9:e1000575. 2011
    ..Together, our results demonstrate that the lipoprotein receptor LRP1, which is critical in lipid metabolism, also regulates food intake and energy homeostasis in the adult central nervous system...
  37. pmc MicroRNA-205 inhibits tumor cell migration through down-regulating the expression of the LDL receptor-related protein 1
    Heesang Song
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Biochem Biophys Res Commun 388:400-5. 2009
    ..These results, for the first time, demonstrate that expression of human LRP1 is regulated in part by a specific miRNA, leading to decreased tumor cell migration...
  38. ncbi request reprint Low density lipoprotein (LDL) receptor-related protein 1B impairs urokinase receptor regeneration on the cell surface and inhibits cell migration
    Yonghe Li
    Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, Missouri 63110, USA
    J Biol Chem 277:42366-71. 2002
    ..Taken together, these results demonstrate that LRP1B can function as a negative regulator of uPAR regeneration and cell migration...
  39. ncbi request reprint Cellular catabolism of lipid poor apolipoprotein E via cell surface LDL receptor-related protein
    Masaaki Narita
    Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, St Louis, Missouri 63110, USA
    J Biochem 132:743-9. 2002
    ....
  40. ncbi request reprint LRP and Alzheimer's disease
    Celina V Zerbinatti
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO, USA
    Rev Neurosci 16:123-35. 2005
    ..Finally, LRP has been linked to AD by genetic evidence. In this review we discuss the potential mechanisms by which LRP can affect APP and Abeta metabolism, and therefore contribute to the pathogenesis of AD...
  41. pmc Meeting report: 2009 international conference on molecular neurodegeneration May 18-20, 2009, Xiamen, China
    Yunwu Zhang
    Departments of Pediatrics, and Cell Biology and Physiology, Washington University School of Medicine, St, Louis, Missouri, USA
    Mol Neurodegener 4:43. 2009
    ..About 100 investigators presented their recent discoveries with a wide range of scopes of neurodegeneration research, including new genes, molecular pathways, animal models, and potential therapeutics...
  42. ncbi request reprint Receptor-associated protein facilitates proper folding and maturation of the low-density lipoprotein receptor and its class 2 mutants
    Yonghe Li
    Department of Pediatrics, St Louis Children s Hospital, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biochemistry 41:4921-8. 2002
    ..Taken together, our results show that the folding, trafficking, and maturation of the LDLR and its class 2 mutants are promoted by RAP...
  43. pmc Proteasome regulates the delivery of LDL receptor-related protein into the degradation pathway
    Lora Melman
    Department of Pediatrics, Washington University School of Medicine, CB 8208, St Louis Children s Hospital, Missouri 63110, USA
    Mol Biol Cell 13:3325-35. 2002
    ..e., LRP, is regulated by the proteasomal system, suggesting a broader function of the proteasome in regulating the trafficking of receptors into the degradation pathway...
  44. pmc The gamma/sigma1 and alpha/sigma2 hemicomplexes of clathrin adaptors AP-1 and AP-2 harbor the dileucine recognition site
    Balraj Doray
    Department of Internal Medicine and Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 18:1887-96. 2007
    ..These findings provide new insights into the trafficking mechanisms of D/EXXXL[LI]-mediated sorting signals...
  45. ncbi request reprint Essential role of the low density lipoprotein receptor-related protein in vascular smooth muscle cell migration
    Yonghe Li
    Department of Pediatrics, Washington University School of Medicine and St Louis Children s Hospital, St Louis, MO 63110, USA
    FEBS Lett 555:346-50. 2003
    ..More important, we found that platelet-derived growth factor-dependent cell migration was inhibited in cells transfected with LRP siRNA. These results demonstrate an important role of LRP in smooth muscle cell migration...
  46. pmc Increased soluble amyloid-beta peptide and memory deficits in amyloid model mice overexpressing the low-density lipoprotein receptor-related protein
    Celina V Zerbinatti
    Departments of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 101:1075-80. 2004
    ..These results provide in vivo evidence that LRP may contribute to memory deficits typical of Alzheimer's disease by modulating the pool of small soluble forms of Abeta...
  47. pmc LRP1 shedding in human brain: roles of ADAM10 and ADAM17
    Qiang Liu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Neurodegener 4:17. 2009
    ..abstract:..
  48. ncbi request reprint LRP5, a multifunctional cell surface receptor
    Yonghe Li
    Department of Pediatrics, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Curr Opin Lipidol 15:361-3. 2004
  49. ncbi request reprint Lipoprotein receptor binding, cellular uptake, and lysosomal delivery of fusions between the receptor-associated protein (RAP) and alpha-L-iduronidase or acid alpha-glucosidase
    William S Prince
    BioMarin Pharmaceutical, Inc, Novato, CA 94949, USA
    J Biol Chem 279:35037-46. 2004
    ..The advantages of the lipoprotein receptor system over oligosaccharide receptor systems include more efficient cellular delivery and the potential for transcytosis of ligands across tight endothelia, including the blood-brain barrier...
  50. pmc Nuclear targeting by the growth factor midkine
    Yoshihisa Shibata
    Department of Biochemistry, Nagoya University School of Medicine, Showa Ku, Japan
    Mol Cell Biol 22:6788-96. 2002
    ..This study therefore reveals a novel role of LRP in intracellular signaling by its ligand and the importance of nucleolin in this process...
  51. pmc Ligand-independent growth hormone receptor dimerization occurs in the endoplasmic reticulum and is required for ubiquitin system-dependent endocytosis
    Jürgen Gent
    Department of Cell Biology and Institute of Biomembranes, University Medical Center, 3584 CX, Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 99:9858-63. 2002
    ..Internalization of a chimeric receptor, which fails to dimerize, is independent of an intact UbE-motif. Therefore, we postulate that dimerization of GHR molecules is required for ubiquitin system-dependent endocytosis...
  52. ncbi request reprint Low density lipoprotein receptor-related protein mediates apolipoprotein E inhibition of smooth muscle cell migration
    Debi K Swertfeger
    Center for Lipid and Arteriosclerosis Studies, Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, USA
    J Biol Chem 277:4141-6. 2002
    ..Taken together, these results document that apoE binding to LRP is required for its inhibition of PDGF-directed smooth muscle cell migration...
  53. ncbi request reprint Upregulation of forebrain NMDA NR2B receptors contributes to behavioral sensitization after inflammation
    Long Jun Wu
    Department of Physiology, Faculty of Medicine, University of Toronto Centre for the Study of Pain, Toronto, Ontario, M5S 1A8, Canada
    J Neurosci 25:11107-16. 2005
    ..Our results demonstrate that the upregulation of NR2B receptors in the ACC contributes to behavioral sensitization caused by inflammation...
  54. ncbi request reprint Is tissue-type plasminogen activator a neuromodulator?
    Monica Fernandez-Monreal
    University of Caen, CNRS, 14047, Caen, France
    Mol Cell Neurosci 25:594-601. 2004
    ..released t-PA modulates NMDA receptor signaling and (iv). astrocytes are able to recapture extracellular t-PA through a low-density lipoprotein (LDL) receptor-related protein (LRP)-dependent mechanism...
  55. ncbi request reprint Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP)
    Hua Poo Su
    Beirne Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:11772-9. 2002
    ..These studies suggest a physical link between CED-1 or CD91/LRP and the adapter protein CED-6/GULP during engulfment of apoptotic cells and further elucidate the pathway suggested by the genetic studies...
  56. ncbi request reprint ApoER2 is endocytosed by a clathrin-mediated process involving the adaptor protein Dab2 independent of its Rafts' association
    Loreto Cuitino
    FONDAP Center for Cell Regulation and Pathology, Joaquín V Luco, Department of Cell and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Traffic 6:820-38. 2005
    ..These studies indicate that apoER2 internalizes through a clathrin-mediated pathway and that its association with caveolar and noncaveolar rafts does not determine its endocytosis...
  57. ncbi request reprint LRP 1 B functions as a receptor for Pseudomonas exotoxin
    Diana V Pastrana
    Laboratory of Molecular Biology, CCR, National Cancer Institute, Bethesda, MD 20892 4263, USA
    Biochim Biophys Acta 1741:234-9. 2005
    ..Previously, low-density lipoprotein receptor-related protein 1 (LRP 1) was shown to be the primary receptor for PE. In this report, we show that a close family member, LRP 1B, can also function as a receptor...
  58. ncbi request reprint Tissue-type plasminogen activator crosses the intact blood-brain barrier by low-density lipoprotein receptor-related protein-mediated transcytosis
    Karim Benchenane
    INSERM Avenir tPA in the working brain, Universite de Caen, GIP Cyceron, Caen, France
    Circulation 111:2241-9. 2005
    ..Determining whether and how vascular tPA can cross the blood-brain barrier (BBB) to enter the brain is thus important, not only during stroke but also in physiological conditions...
  59. ncbi request reprint A cytoplasmic PPPSP motif determines megalin's phosphorylation and regulates receptor's recycling and surface expression
    Maria Isabel Yuseff
    FONDAP Center for Cell Regulation and Pathology CRCP, Departamento de Biologia Celular y Molecular, Facultad de Ciencias Biologicas, Pontificia Universidad Católica de Chile and MIFAB, Santiago, Chile
    Traffic 8:1215-30. 2007
    ..Altogether, these results show that the PPPSP motif and the GSK3 activity are critical to allow megalin phosphorylation and also negatively regulate the receptor's recycling...
  60. ncbi request reprint LRP5/6 in Wnt signaling and tumorigenesis
    Yonghe Li
    Southern Research Institute, Biochemistry and Molecular Biology Department, Drug Discovery Division, Birmingham, AL 35255, USA
    Future Oncol 1:673-81. 2005
    ..Furthermore, it has been demonstrated that LRP5 and 6 are potential oncogenic proteins. Thus, blockade of LRP56 function may lead to new strategies in cancer treatment...
  61. ncbi request reprint Midkine and LDL-receptor-related protein 1 contribute to the anchorage-independent cell growth of cancer cells
    Sen Chen
    Department of Biochemistry, Nagoya University School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Cell Sci 120:4009-15. 2007
    ..Collectively, these data demonstrate a crucial role of MK-LRP1 signaling in anchorage-independent cell growth...
  62. doi request reprint LRP1 controls biosynthetic and endocytic trafficking of neuronal prion protein
    Celia J Parkyn
    King s College London, Wolfson Centre for Age Related Disease, Guy s Campus, London SE1 1UL, UK
    J Cell Sci 121:773-83. 2008
    ..Taken together, these data argue that LRP1 controls both the surface, and biosynthetic, trafficking of PrPC in neurons...
  63. pmc Lipoprotein receptor-related protein-1 mediates amyloid-beta-mediated cell death of cerebrovascular cells
    Micha M M Wilhelmus
    Department of Neurology and Alzheimer Center, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Am J Pathol 171:1989-99. 2007
    ..Although perivascular cells may adapt their A beta internalization capacity to the levels of A beta present, saturated LRP-1/LDLR-mediated uptake of A beta results in degeneration of perivascular cells...
  64. ncbi request reprint Efficient transfer of receptor-associated protein (RAP) across the blood-brain barrier
    Weihong Pan
    Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge 70808, USA
    J Cell Sci 117:5071-8. 2004
    ..These studies suggest that RAP may provide a novel means of protein-based drug delivery to the brain...
  65. pmc Self-assembly of HEK cell-secreted ApoE particles resembles ApoE enrichment of lipoproteins as a ligand for the LDL receptor-related protein
    Mary Jo Ladu
    Department of Anatomy and Cell Biology, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    Biochemistry 45:381-90. 2006
    ..Our results indicate that, in addition to lipid content, the assembly state of apoE influences Abeta binding and receptor recognition...
  66. ncbi request reprint Morphogens, new passengers on lipoprotein particles
    Maria Paz Marzolo
    Curr Opin Lipidol 17:202-4. 2006
  67. ncbi request reprint Defective thyroglobulin storage in LDL receptor-associated protein-deficient mice
    Simonetta Lisi
    Department of Endocrinology, University of Pisa, Via Paradisa 2, I 56124 Pisa, Italy
    Am J Physiol Cell Physiol 290:C1160-7. 2006
    ..In conclusion, RAP is required for the establishment of Tg reservoirs, but its absence does not affect hormone secretion...
  68. ncbi request reprint Genetics and molecular biology: motive studies in mice--two NPXY motifs in the intracellular domain of lipoprotein receptor-related protein 1 exhibit differential importance during fetal development
    Jane M Knisely
    Curr Opin Lipidol 17:480-2. 2006
  69. ncbi request reprint Genetics and molecular biology: phosphorylation of low-density lipoprotein receptor-related protein by platelet-derived growth factor
    Pattie S Green
    Curr Opin Lipidol 13:569-72. 2002
  70. ncbi request reprint Genetics and molecular biology
    Jane M Knisely
    Curr Opin Lipidol 14:401-3. 2003
  71. ncbi request reprint Differential distribution of low-density lipoprotein-receptor-related protein (LRP) and megalin in polarized epithelial cells is determined by their cytoplasmic domains
    Maria Paz Marzolo
    Centro de Regulación Celular y Patología, Departamento de Biologia Celular y Molecular, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Traffic 4:273-88. 2003
    ..Additionally, we show that the NPTY motif in LRP is important for basolateral sorting and the megalin transmembrane domain directs association with lipid rafts...
  72. pmc tPA protects renal interstitial fibroblasts and myofibroblasts from apoptosis
    Kebin Hu
    Department of Pathology, University of Pittsburgh, S 405 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15261, USA
    J Am Soc Nephrol 19:503-14. 2008
    ..Together, these findings illustrate that tPA is a survival factor that prevents apoptosis of renal interstitial fibroblasts and myofibroblasts through an LRP-1-, Erk1/2-, p90RSK-, and Bad-dependent mechanism...
  73. ncbi request reprint Tissue factor pathway inhibitor induces expression of JUNB and GADD45B mRNAs
    Hiroko Shirotani-Ikejima
    National Cardiovascular Center Research Institute, 5 7 1 Fujishirodai, Suita, Osaka 565 8565, Japan
    Biochem Biophys Res Commun 299:847-52. 2002
    ..In conclusion, this is the first report to show an effect of TFPI on mRNA expression, and suggests that TFPI modulates cellular functions by inducing JUNB and GADD45B expression through binding to VLDL-R...

Research Grants25

  1. Molecular Pathobiology of LRP1B Tumor Suppressor
    Guojun Bu; Fiscal Year: 2004
    ..abstract_text> ..
  2. Molecular Pathobiology of LRP1B Tumor Suppressor
    Guojun Bu; Fiscal Year: 2005
    ..abstract_text> ..
  3. Molecular Pathobiology of LRP1B Tumor Suppressor
    Guojun Bu; Fiscal Year: 2006
    ..abstract_text> ..
  4. Molecular Pathobiology of LRP1B Tumor Suppressor
    Guojun Bu; Fiscal Year: 2007
    ..abstract_text> ..
  5. LRP AND APP PROCESSING IN NEURODEGENERATION
    Guojun Bu; Fiscal Year: 2009
    ..Because apoE is a major risk factor for Alzheimer's disease, a leading cause of dementia in elderly, our results may provide knowledge for diagnosis and/or therapy for Alzheimer's disease. ..
  6. RAP AS A MOLECULAR CHAPERONE/ESCORT PROTEIN FOR LRP
    Guojun Bu; Fiscal Year: 2002
    ....
  7. RAP AS A MOLECULAR CHAPERONE/ESCORT PROTEIN FOR LRP
    Guojun Bu; Fiscal Year: 1999
    ....
  8. NEUROTROPHIN REGULATION OF LRP IN CNS NEURONS
    Guojun Bu; Fiscal Year: 1999
    ....
  9. RAP AS A MOLECULAR CHAPERONE/ESCORT PROTEIN FOR LRP
    Guojun Bu; Fiscal Year: 2000
    ....
  10. NEUROTROPHIN REGULATION OF LRP IN CNS NEURONS
    Guojun Bu; Fiscal Year: 2000
    ....
  11. RAP AS A MOLECULAR CHAPERONE/ESCORT PROTEIN FOR LRP
    Guojun Bu; Fiscal Year: 2001
    ....
  12. NEUROTROPHIN REGULATION OF LRP IN CNS NEURONS
    Guojun Bu; Fiscal Year: 2001
    ....
  13. ApoE Receptor Pathways to Intraneuronal Abeta
    Guojun Bu; Fiscal Year: 2007
    ....
  14. ApoE Receptor Pathways to Intraneuronal Abeta
    Guojun Bu; Fiscal Year: 2006
    ....
  15. ApoE Receptor Pathways to Intraneuronal Abeta
    Guojun Bu; Fiscal Year: 2009
    ....