Robert Putnak

Summary

Affiliation: Walter Reed Army Medical Center
Country: USA

Publications

  1. ncbi request reprint Vaccination of rhesus macaques against dengue-2 virus with a plasmid DNA vaccine encoding the viral pre-membrane and envelope genes
    Robert Putnak
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910 7500, USA
    Am J Trop Med Hyg 68:469-76. 2003
  2. ncbi request reprint Protection of Rhesus monkeys against dengue virus challenge after tetravalent live attenuated dengue virus vaccination
    Wellington Sun
    Department of Virus Diseases, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910 7500, USA
    J Infect Dis 193:1658-65. 2006
  3. ncbi request reprint An evaluation of dengue type-2 inactivated, recombinant subunit, and live-attenuated vaccine candidates in the rhesus macaque model
    J Robert Putnak
    Walter Reed Army Institute of Research, Division of Communicable Diseases and Immunology, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Vaccine 23:4442-52. 2005
  4. ncbi request reprint Comparative evaluation of three assays for measurement of dengue virus neutralizing antibodies
    J Robert Putnak
    Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 79:115-22. 2008
  5. doi request reprint Intra-genotypic variation of predominant genotype II strains of dengue type-3 virus isolated during different epidemics in Thailand from 1973 to 2001
    Aimee Zhang
    Science and Engineering Apprentice Program at the Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Virus Genes 46:203-18. 2013
  6. ncbi request reprint Modification of dengue virus strains by passage in primary dog kidney cells: preparation of candidate vaccines and immunization of monkeys
    Kenneth H Eckels
    Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 69:12-6. 2003
  7. ncbi request reprint DNA vaccines for flaviviruses
    Robert Putnak
    Division of Communicable Diseases and Immunology, Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Adv Virus Res 61:445-68. 2003
  8. doi request reprint Letter to the editor: molecular genotyping of Dengue Virus Types 2 and 4 from the Guatemalan and Honduran Epidemics of 2007 using the envelope glycoprotein gene
    Yuxin Tang
    Division of Viral Diseases, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    Virus Genes 42:200-3. 2011
  9. doi request reprint Protection against dengue virus by non-replicating and live attenuated vaccines used together in a prime boost vaccination strategy
    Monika Simmons
    Viral and Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, MD 20910, USA
    Virology 396:280-8. 2010
  10. pmc Characterization of antibody responses to combinations of a dengue virus type 2 DNA vaccine and two dengue virus type 2 protein vaccines in rhesus macaques
    Monika Simmons
    Viral Diseases Department, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    J Virol 80:9577-85. 2006

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Vaccination of rhesus macaques against dengue-2 virus with a plasmid DNA vaccine encoding the viral pre-membrane and envelope genes
    Robert Putnak
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910 7500, USA
    Am J Trop Med Hyg 68:469-76. 2003
    ..Long-term protection following DNA vaccination may require revaccination, higher doses of DNA, or a vaccine that contains additional epitopes or adjuvants...
  2. ncbi request reprint Protection of Rhesus monkeys against dengue virus challenge after tetravalent live attenuated dengue virus vaccination
    Wellington Sun
    Department of Virus Diseases, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910 7500, USA
    J Infect Dis 193:1658-65. 2006
    ..Interference among the 4 vaccine viruses may have resulted in decreased antibody responses to DENV-3 and -4, which would require reformulation or dose optimization to minimize this interference during testing of the vaccine in humans...
  3. ncbi request reprint An evaluation of dengue type-2 inactivated, recombinant subunit, and live-attenuated vaccine candidates in the rhesus macaque model
    J Robert Putnak
    Walter Reed Army Institute of Research, Division of Communicable Diseases and Immunology, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Vaccine 23:4442-52. 2005
    ..Several groups exhibited nearly complete protection against viremia by bioassay, although there was evidence for challenge virus replication by Taqmantrade mark and immunological assays. None of the vaccines conferred sterile immunity...
  4. ncbi request reprint Comparative evaluation of three assays for measurement of dengue virus neutralizing antibodies
    J Robert Putnak
    Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 79:115-22. 2008
    ..However, when used to test sera from persons after tetravalent DENV vaccination or secondary DENV infection, there was poor specificity and poor agreement among the different assays...
  5. doi request reprint Intra-genotypic variation of predominant genotype II strains of dengue type-3 virus isolated during different epidemics in Thailand from 1973 to 2001
    Aimee Zhang
    Science and Engineering Apprentice Program at the Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Virus Genes 46:203-18. 2013
    ....
  6. ncbi request reprint Modification of dengue virus strains by passage in primary dog kidney cells: preparation of candidate vaccines and immunization of monkeys
    Kenneth H Eckels
    Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 69:12-6. 2003
    ..A DENV-3 strain lost all monkey infectivity after PDK cell passage. Twelve vaccine candidates were selected for Phase 1 human trials through this selection process...
  7. ncbi request reprint DNA vaccines for flaviviruses
    Robert Putnak
    Division of Communicable Diseases and Immunology, Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Adv Virus Res 61:445-68. 2003
  8. doi request reprint Letter to the editor: molecular genotyping of Dengue Virus Types 2 and 4 from the Guatemalan and Honduran Epidemics of 2007 using the envelope glycoprotein gene
    Yuxin Tang
    Division of Viral Diseases, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    Virus Genes 42:200-3. 2011
    ....
  9. doi request reprint Protection against dengue virus by non-replicating and live attenuated vaccines used together in a prime boost vaccination strategy
    Monika Simmons
    Viral and Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, MD 20910, USA
    Virology 396:280-8. 2010
    ..After challenge with each one of the four DENV serotypes, vaccinated animals exhibited no viremia but showed anamnestic antibody responses to the challenge viruses...
  10. pmc Characterization of antibody responses to combinations of a dengue virus type 2 DNA vaccine and two dengue virus type 2 protein vaccines in rhesus macaques
    Monika Simmons
    Viral Diseases Department, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    J Virol 80:9577-85. 2006
    ..5 days), which also showed the highest antibody concentration, including antibodies to NS1, and highest antibody avidity at the time of challenge...
  11. pmc A phase II, randomized, safety and immunogenicity study of a re-derived, live-attenuated dengue virus vaccine in healthy adults
    Stephen J Thomas
    Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 88:73-88. 2013
    ..Tetravalent response rates to the new formulations were 60% and 66.7% in unprimed subjects. A third dose did not increase tetravalent antibody rates. The new DENV vaccine candidates merit additional evaluation...
  12. ncbi request reprint A Phase 2 study of a purified, inactivated virus vaccine to prevent Japanese encephalitis
    Arthur Lyons
    Department of Virus Diseases, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Vaccine 25:3445-53. 2007
    ..JE-PIV was well tolerated, resulted in high seroconversion rates [Day 56 (primary endpoint); 95-100%] and induced enduring immune responses up to 2 years after vaccination. Expanded Phase 3 trials are planned...
  13. ncbi request reprint Evidence for inter- and intra-genotypic variations in dengue serotype 4 viruses representing predominant and non-predominant genotypes co-circulating in Thailand from 1977 to 2001
    Richard Zhao
    Science and Engineering Apprentice Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Virus Genes 41:5-13. 2010
    ..The conserved 3'-SL structure may be a target for antiviral drug development...
  14. ncbi request reprint Phase 2 clinical trial of three formulations of tetravalent live-attenuated dengue vaccine in flavivirus-naïve adults
    Wellington Sun
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA
    Hum Vaccin 5:33-40. 2009
    ..Formulation 17 was selected for further clinical evaluation based on this study...
  15. ncbi request reprint Vaccination of human volunteers with monovalent and tetravalent live-attenuated dengue vaccine candidates
    Wellington Sun
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 69:24-31. 2003
    ..A third dose of the tetravalent vaccine at four months resulted in three of four volunteers with trivalent or tetravalent high-titer neutralizing antibody responses...
  16. doi request reprint Phenotypic analysis of dengue virus isolates associated with dengue fever and dengue hemorrhagic fever for cellular attachment, replication and interferon signaling ability
    Ratree Takhampunya
    Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Virus Res 145:31-8. 2009
    ..Our results suggest that the emergence early in infection of viruses with a high degree of replication fitness may play an important role in DENV pathogenesis...
  17. pmc Comparative analysis of full-length genomic sequences of 10 dengue serotype 1 viruses associated with different genotypes, epidemics, and disease severity isolated in Thailand over 22 years
    Yuxin Tang
    Division of Viral Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 83:1156-65. 2010
    ....
  18. ncbi request reprint Formalin-inactivated whole virus and recombinant subunit flavivirus vaccines
    Kenneth H Eckels
    Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Adv Virus Res 61:395-418. 2003
    ..Feasibility of non-replicating flavivirus vaccines has been clearly demonstrated and further development is now warranted...
  19. ncbi request reprint Safety and immunogenicity of a tetravalent live-attenuated dengue vaccine in flavivirus naive children
    Sriluck Simasathien
    Department of Pediatrics, Phramongkutklao Hospital, Bangkok, Thailand
    Am J Trop Med Hyg 78:426-33. 2008
    ..gov NCT00384670)...