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Genomes and Genes | William C NiermanSummaryAffiliation: Walter Reed Army Medical Center Country: USA Publications
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Publications
Genome sequence alterations detected upon passage of Burkholderia mallei ATCC 23344 in culture and in mammalian hostsClaudia M Romero
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
BMC Genomics 7:228. 2006..mallei ATCC 23344 isolates, one from each source, were sequenced from culture, a mouse, a horse, and two isolates from a single human patient, and the sequence compared to the published B. mallei ATCC 23344 genome sequence...- Tandem repeat regions within the Burkholderia pseudomallei genome and their application for high resolution genotypingJana M U'ren
Northern Arizona University, Center for Microbial Genetics and Genomics, Flagstaff, Arizona 86011, USA
BMC Microbiol 7:23. 2007..pseudomallei and 21 B. mallei isolates from around the world, as well as 95 lineages from a serial transfer experiment encompassing ~18,000 generations... - Genetic and phenotypic diversity in Burkholderia: contributions by prophage and phage-like elementsCatherine M Ronning
J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
BMC Microbiol 10:202. 2010..These GIs may provide some strains with a competitive advantage in the environment and/or in the host relative to other strains... - Genomic diversity of Burkholderia pseudomallei clinical isolates: subtractive hybridization reveals a Burkholderia mallei-specific prophage in B. pseudomallei 1026bDavid DeShazer
United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702, USA
J Bacteriol 186:3938-50. 2004..The bacteriophage characterized in this study may be a useful diagnostic tool for differentiating B. pseudomallei and B. mallei, two closely related biological threat agents... 
Virulence of clinical and environmental isolates of Burkholderia oklahomensis and Burkholderia thailandensis in hamsters and miceDavid DeShazer
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA
FEMS Microbiol Lett 277:64-9. 2007..The virulence of B. thailandensis CDC3015869, on the other hand, was more similar to the virulence of isolates of B. thailandensis from Southeast Asia...- Identification of a Burkholderia mallei polysaccharide gene cluster by subtractive hybridization and demonstration that the encoded capsule is an essential virulence determinantD DeShazer
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA
Microb Pathog 30:253-69. 2001..The results presented here demonstrate that the capsular polysaccharide of B. mallei is required for production of disease in two animal models of glanders infection and is a major virulence factor... - Structural flexibility in the Burkholderia mallei genomeWilliam C Nierman
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 101:14246-51. 2004..Variation in simple sequence repeats in key genes can provide a mechanism for generating antigenic variation that may account for the mammalian host's inability to mount a durable adaptive immune response to a B. mallei infection... - Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategiesH Stanley Kim
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
BMC Genomics 6:174. 2005..To investigate the genomic factors resulting in such a dramatic difference, we first identified the Bm genes responsive to the mouse environment, and then examined the divergence of these genes in Bp and Bt... - Genomics of Aspergillus fumigatusCatherine M Ronning
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Rev Iberoam Micol 22:223-8. 2005..A. fumigatus was compared with the food biotechnology fungus Aspergillus oryzae and sexual fungus Aspergillus nidulans, as well as other fungi, in an attempt to discern key differences between these organisms... - Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatusWilliam C Nierman
The Institute for Genomic Research, Rockville, Maryland 20850, USA
Nature 438:1151-6. 2005..The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus... - Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatusNatalie D Fedorova
The J Craig Venter Institute, Rockville, Maryland, United States of America
PLoS Genet 4:e1000046. 2008.... - Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in miceRicky L Ulrich
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702, USA
Vaccine 23:1986-92. 2005..Thus, live attenuated strains that promote a Th1-like Ig response may serve as promising vaccine candidates against aerosol infection with B. mallei... - The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteriaTimothy D Read
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 423:81-6. 2003..The complete sequence of B. anthracis is a step towards a better understanding of anthrax pathogenesis... - Mouse model of sublethal and lethal intraperitoneal glanders (Burkholderia mallei)D L Fritz
Pathology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702 5011, USA
Vet Pathol 37:626-36. 2000..Additionally, by immunoelectron microscopy, we showed the presence of type I O-antigenic polysaccharide (capsular) antigen surrounding B. mallei... - Genome sequencing and analysis of Yersina pestis KIM D27, an avirulent strain exempt from select agent regulationLiliana Losada
J Craig Venter Institute, Rockville, Maryland, United States of America
PLoS ONE 6:e19054. 2011..For purposes of obtaining high quality genome sequence differences between strains, any identified differences should be verified in both the new and reference genomes... 
Using aCGH to study intraspecific genetic variability in two pathogenic molds, Aspergillus fumigatus and Aspergillus flavusNatalie D Fedorova
The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
Med Mycol 47:S34-41. 2009..Preliminary evidence suggests that gene flow in both species is largely constrained by VCG boundaries, although further VCG sampling is required to confirm this observation...- Continuing Evolution of Burkholderia mallei Through Genome Reduction and Large-Scale RearrangementsLiliana Losada
J Craig Venter Institute, Rockville, Maryland
Genome Biol Evol 2010:102-16. 2010.... - The power in comparisonWilliam C Nierman
The Institute for Genomic Research, Rockville, MD 20850, USA
Trends Microbiol 12:62-3. 2004 - Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related BurkholderiaceaeRicky L Ulrich
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA
Diagn Microbiol Infect Dis 55:37-45. 2006..mallei. These primers were used to develop a rapid PCR assay for the definitive identification of B. mallei and differentiation from all other bacteria... - Transcriptional divergence of the duplicated oxidative stress-responsive genes in the Arabidopsis genomeH Stanley Kim
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Plant J 41:212-20. 2005.... - Human, mouse, and rat genome large-scale rearrangements: stability versus speciationShaying Zhao
Institute for Genomic Research, Rockville, Maryland 20850, USA
Genome Res 14:1851-60. 2004.... - Effect of competition on the production and activity of secondary metabolites in Aspergillus speciesLiliana Losada
J Craig Venter Institute, Rockville, Maryland 20850, USA
Med Mycol 47:S88-96. 2009..These findings indicate that co-cultivation could be a very promising method for inducing and characterizing novel antifungal compounds produced by species of Aspergillus... - Polysaccharide microarray technology for the detection of Burkholderia pseudomallei and Burkholderia mallei antibodiesNarayanan Parthasarathy
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA
Diagn Microbiol Infect Dis 56:329-32. 2006..pseudomallei and B. mallei serum (human and animal) antibodies. The advantages of this microarray technology over the current serodiagnosis of the above bacterial infections were discussed... - Detection of the host immune response to Burkholderia mallei heat-shock proteins GroEL and DnaK in a glanders patient and infected miceKei Amemiya
Bacteriology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA
Diagn Microbiol Infect Dis 59:137-47. 2007..mallei showed that more GroEL protein than DnaK protein was released from the cell. This may occur similarly within an infected host causing an elevated host immune response to the B. mallei hsps... - The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomalleiMary N Burtnick
Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama 36688, USA
Infect Immun 79:1512-25. 2011..7 macrophages 18 h after infection. The results suggest that the cluster 1 T6SS is essential for virulence and plays an important role in the intracellular lifestyle of B. pseudomallei... - Quorum sensing: a transcriptional regulatory system involved in the pathogenicity of Burkholderia malleiRicky L Ulrich
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter St, Fort Detrick, MD 21702, USA
Infect Immun 72:6589-96. 2004..These findings demonstrate that B. mallei carries multiple luxIR homologues that either directly or indirectly regulate the biosynthesis of an essential virulence factor(s) that contributes to the pathogenicity of B. mallei in vivo... - Nonviable Burkholderia mallei induces a mixed Th1- and Th2-like cytokine response in BALB/c miceKei Amemiya
Pathogenesis and Immunology Branch, Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702, USA
Infect Immun 70:2319-25. 2002..This may be the reason for the inability of the B. mallei cells that were examined as candidate vaccines to protect the mice from a live challenge... - Type III secretion: a virulence factor delivery system essential for the pathogenicity of Burkholderia malleiRicky L Ulrich
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA
Infect Immun 72:1150-4. 2004..However, vaccination with each mutant failed to elicit a protective immunity against challenge with wild-type ATCC 23344... - Role of quorum sensing in the pathogenicity of Burkholderia pseudomalleiRicky L Ulrich
Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702 5011, USA
J Med Microbiol 53:1053-64. 2004..pseudomallei. The findings of the investigation demonstrate that B. pseudomallei encodes multiple luxIR genes, and disruption of the QS alleles reduces animal pathogenicity, but does not affect exoproduct secretion... - Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytesMalcolm J Gardner
Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:134-7. 2005..Several biosynthetic pathways are incomplete or absent, suggesting substantial metabolic dependence on the host cell. One protein family that may generate parasite antigenic diversity is not telomere-associated... - Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomalleiD DeShazer
Genetics and Physiology Branch, Bacteriology Division, U S Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702, USA
J Bacteriol 181:4661-4. 1999..In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC... - Exogenous Yersinia pestis quorum sensing molecules N-octanoyl-homoserine lactone and N-(3-oxooctanoyl)-homoserine lactone regulate the LcrV virulence factorH Carl Gelhaus
United States Army Medical Institute of Infectious Diseases, Bacteriology Division, 1425 Porter St, 21702 Ft Detrick, MD, USA
Microb Pathog 46:283-7. 2009..DNA microarray analysis shows 10 additional T3SS genes are consistently down-regulated by C8 or oxo-C8. This is the first report demonstrating that AHLs regulate Y. pestis virulence factor expression... - Comparative analysis of programmed cell death pathways in filamentous fungiNatalie D Fedorova
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
BMC Genomics 6:177. 2005.... - Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elementsMartin Wu
The Institute for Genomic Research, Rockville, Maryland, USA
PLoS Biol 2:E69. 2004..With the availability of the complete genomes of both species and excellent genetic tools for the host, the wMel-D. melanogaster symbiosis is now an ideal system for studying the biology and evolution of Wolbachia infections... - The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacteriumJonathan A Eisen
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Proc Natl Acad Sci U S A 99:9509-14. 2002..tepidum and many Archaeal species... - Genomics for applied microbiologyWilliam C Nierman
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Adv Appl Microbiol 51:201-45. 2002 - Comparative genomics and an insect model rapidly identify novel virulence genes of Burkholderia malleiMark A Schell
Department of Microbiology, University of Georgia, Athens, GA 30602, USA
J Bacteriol 190:2306-13. 2008..Thus, this approach may provide a means to quickly identify high-probability virulence genes in B. pseudomallei, B. mallei, and other pathogens... - Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutantJoanne M Stevens
Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, UK
J Bacteriol 187:7857-62. 2005..pseudomallei bimA mutant. While the BimA homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind Arp3... - Genomic patterns of pathogen evolution revealed by comparison of Burkholderia pseudomallei, the causative agent of melioidosis, to avirulent Burkholderia thailandensisYiting Yu
Genome Institute of Singapore, Singapore 138672, Republic of Singapore
BMC Microbiol 6:46. 2006..To understand the evolutionary mechanisms contributing to Bp virulence, we performed a comparative genomic analysis of Bp K96243 and B. thailandensis (Bt) E264, a closely related but avirulent relative... - A disruption of ctpA encoding carboxy-terminal protease attenuates Burkholderia mallei and induces partial protection in CD1 miceAloka B Bandara
Center for Molecular Medicine and Infectious Diseases, Virginia Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 0342, USA
Microb Pathog 45:207-16. 2008..mallei ATCC 23344. These findings suggest that CtpA regulates in vitro growth, cell morphology and intracellular survival of B. mallei, and a ctpA mutant protects CD1 mice against glanders... - Management of accidental laboratory exposure to Burkholderia pseudomallei and B. malleiSharon J Peacock
Mahidol University, Bangkok, Thailand
Emerg Infect Dis 14:e2. 2008..pseudomallei. The overall mortality rate of infected persons is 50% in northeast Thailand (35% in children) and 19% in Australia... - Characterization of clinically-attenuated Burkholderia mallei by whole genome sequencing: candidate strain for exclusion from Select Agent listsSteven E Schutzer
Department of Medicine, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey, United States of America
PLoS ONE 3:e2058. 2008..Whole genomic sequencing (WGS) is an apt approach to characterize newly discovered or poorly understood microbial pathogens... - Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomalleiMatthew T G Holden
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
Proc Natl Acad Sci U S A 101:14240-5. 2004..mallei. We propose that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species... - Contribution of gene loss to the pathogenic evolution of Burkholderia pseudomallei and Burkholderia malleiRichard A Moore
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
Infect Immun 72:4172-87. 2004..The increase in virulence associated with the loss of these genes may have provided a selective advantage for B. pseudomallei as these organisms adapted to survival in animal hosts... - Type VI secretion is a major virulence determinant in Burkholderia malleiMark A Schell
Department of Microbiology, University of Georgia, Athens, GA 30602, USA
Mol Microbiol 64:1466-85. 2007..Purified His-tagged BMAA0742 was recognized by glanders antiserum from a horse, a human and mice, indicating that this Hcp-family protein is produced in vivo during infection... - Characterization of experimental equine glandersJose Lopez
National Centre for Foreign Animal Disease, 1015 Arlington St, Suite T2300, Winnipeg, Man, Canada R3E 3M4
Microbes Infect 5:1125-31. 2003..mallei produced clinical glanders with organisms being recovered from tissues of infected horses. The model should prove to be of considerable value in our ongoing studies on the pathogenesis and vaccine development for glanders... - Burkholderia thailandensis E125 harbors a temperate bacteriophage specific for Burkholderia malleiDonald E Woods
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Alberta T2N 4N1, Canada
J Bacteriol 184:4003-17. 2002..The results presented here demonstrate that phiE125 is a new member of the lambda supergroup of Siphoviridae that may be useful as a diagnostic tool for B. mallei... - mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344Kyoung-Hee Choi
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA
Nat Protoc 1:162-9. 2006..This first chromosome integration system in B. mallei provides an important contribution to the genetic tools emerging for Burkholderia spp. Vectors are available for gene complementation and expression, and gene fusion analyses...