Evelina Angov

Summary

Affiliation: Walter Reed Army Medical Center
Country: USA

Publications

  1. doi request reprint Adjustment of codon usage frequencies by codon harmonization improves protein expression and folding
    Evelina Angov
    Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Methods Mol Biol 705:1-13. 2011
  2. pmc Results from tandem Phase 1 studies evaluating the safety, reactogenicity and immunogenicity of the vaccine candidate antigen Plasmodium falciparum FVO merozoite surface protein-1 (MSP1(42)) administered intramuscularly with adjuvant system AS01
    Nekoye Otsyula
    Walter Reed Project, Kenya Medical Research Institute, Kisumu, Kenya
    Malar J 12:29. 2013
  3. pmc Marked variation in MSP-119 antibody responses to malaria in western Kenyan highlands
    Kingsley Badu
    Center for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya
    BMC Infect Dis 12:50. 2012
  4. pmc Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host
    Evelina Angov
    Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 3:e2189. 2008
  5. pmc Codon usage: nature's roadmap to expression and folding of proteins
    Evelina Angov
    Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Biotechnol J 6:650-9. 2011
  6. pmc Phase 1/2a study of the malaria vaccine candidate apical membrane antigen-1 (AMA-1) administered in adjuvant system AS01B or AS02A
    Michele D Spring
    United States Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 4:e5254. 2009
  7. pmc Protection induced by Plasmodium falciparum MSP1(42) is strain-specific, antigen and adjuvant dependent, and correlates with antibody responses
    Jeffrey A Lyon
    Division Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 3:e2830. 2008
  8. pmc Comparison of Plasmodium berghei challenge models for the evaluation of pre-erythrocytic malaria vaccines and their effect on perceived vaccine efficacy
    Wolfgang W Leitner
    Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Malar J 9:145. 2010
  9. doi request reprint Histidine affinity tags affect MSP1(42) structural stability and immunodominance in mice
    Farhat Khan
    Walter Reed Army Institute of Research, Military Malaria Research Program, Malaria Vaccine Branch, Silver Spring, MD, USA
    Biotechnol J 7:133-47. 2012
  10. doi request reprint Cellular and humoral immune effector mechanisms required for sterile protection against sporozoite challenge induced with the novel malaria vaccine candidate CelTOS
    Elke S Bergmann-Leitner
    Division of Malaria Vaccine Development, US Military Malaria Vaccine Program, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    Vaccine 29:5940-9. 2011

Collaborators

Detail Information

Publications37

  1. doi request reprint Adjustment of codon usage frequencies by codon harmonization improves protein expression and folding
    Evelina Angov
    Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Methods Mol Biol 705:1-13. 2011
    ..Although so far exclusively applied to Escherichia coli, codon harmonization may provide a general strategy for improving the expression of soluble, functional proteins during heterologous host expression...
  2. pmc Results from tandem Phase 1 studies evaluating the safety, reactogenicity and immunogenicity of the vaccine candidate antigen Plasmodium falciparum FVO merozoite surface protein-1 (MSP1(42)) administered intramuscularly with adjuvant system AS01
    Nekoye Otsyula
    Walter Reed Project, Kenya Medical Research Institute, Kisumu, Kenya
    Malar J 12:29. 2013
    ....
  3. pmc Marked variation in MSP-119 antibody responses to malaria in western Kenyan highlands
    Kingsley Badu
    Center for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya
    BMC Infect Dis 12:50. 2012
    ..An improved understanding of the micro-geographic variation in malaria exposure in the highland ecosystems will be relevant in planning effective malaria control...
  4. pmc Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression host
    Evelina Angov
    Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 3:e2189. 2008
    ..Codon harmonization may further provide a general strategy for improving the expression of soluble functional proteins during heterologous expression in hosts other than E. coli...
  5. pmc Codon usage: nature's roadmap to expression and folding of proteins
    Evelina Angov
    Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Biotechnol J 6:650-9. 2011
    ..Thus, the focus of this review is to provide some experimental support and a brief overview of how codon usage bias has evolved relative to regulating gene expression levels...
  6. pmc Phase 1/2a study of the malaria vaccine candidate apical membrane antigen-1 (AMA-1) administered in adjuvant system AS01B or AS02A
    Michele D Spring
    United States Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 4:e5254. 2009
    ....
  7. pmc Protection induced by Plasmodium falciparum MSP1(42) is strain-specific, antigen and adjuvant dependent, and correlates with antibody responses
    Jeffrey A Lyon
    Division Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 3:e2830. 2008
    ..Development of an effective MSP1(42) vaccine against erythrocytic stage P. falciparum infection will depend not only on antigen quality, but also upon the selection of an optimal adjuvant component...
  8. pmc Comparison of Plasmodium berghei challenge models for the evaluation of pre-erythrocytic malaria vaccines and their effect on perceived vaccine efficacy
    Wolfgang W Leitner
    Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Malar J 9:145. 2010
    ..Finding shortcomings of both approaches, an alternative approach was explored, i.e., the subcutaneous challenge...
  9. doi request reprint Histidine affinity tags affect MSP1(42) structural stability and immunodominance in mice
    Farhat Khan
    Walter Reed Army Institute of Research, Military Malaria Research Program, Malaria Vaccine Branch, Silver Spring, MD, USA
    Biotechnol J 7:133-47. 2012
    ..Thus, the usefulness of protein tags may be outweighed by their potential impact on structure and function, stressing the need for caution in their use. See accompanying commentary by Randolph DOI: 10.1002/biot.201100459...
  10. doi request reprint Cellular and humoral immune effector mechanisms required for sterile protection against sporozoite challenge induced with the novel malaria vaccine candidate CelTOS
    Elke S Bergmann-Leitner
    Division of Malaria Vaccine Development, US Military Malaria Vaccine Program, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    Vaccine 29:5940-9. 2011
    ....
  11. pmc Immunization with pre-erythrocytic antigen CelTOS from Plasmodium falciparum elicits cross-species protection against heterologous challenge with Plasmodium berghei
    Elke S Bergmann-Leitner
    Division of Malaria Vaccine Development, United States Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 5:e12294. 2010
    ..Therefore, targeting the immune response to this highly conserved protein and thus potentially interfering with its biological function may result in protection against infection even by heterologous species of Plasmodium...
  12. pmc Process development and analysis of liver-stage antigen 1, a preerythrocyte-stage protein-based vaccine for Plasmodium falciparum
    Collette J Hillier
    Department of Immunology, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring, MD 20910 7500, USA
    Infect Immun 73:2109-15. 2005
    ..Finally, sera taken from people living in a region where malaria is holoendemic recognized LSA-NRC by Western blotting...
  13. ncbi request reprint C3d-defined complement receptor-binding peptide p28 conjugated to circumsporozoite protein provides protection against Plasmodium berghei
    Elke S Bergmann-Leitner
    Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD 20910, United States
    Vaccine 25:7732-6. 2007
    ..We conclude that use of the CR2-binding motif of C3d as molecular adjuvant to CSP results in anti-malaria protective immune response probably by targeting the chimeric protein to CR2...
  14. ncbi request reprint Critical evaluation of different methods for measuring the functional activity of antibodies against malaria blood stage antigens
    Elke S Bergmann-Leitner
    Department of Immunology, CD and I, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 75:437-42. 2006
    ..Analysis based on parasitemia and viability was essential for defining the full range of anti-parasite activities in immune sera...
  15. ncbi request reprint Phase I safety and immunogenicity trial of FMP1/AS02A, a Plasmodium falciparum MSP-1 asexual blood stage vaccine
    Christian F Ockenhouse
    Department of Immunology, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Vaccine 24:3009-17. 2006
    ..This study establishes the foundation to further evaluate and measure the vaccine's ability to reduce morbidity and mortality in target populations directly affected by P. falciparum malaria...
  16. pmc A potent malaria transmission blocking vaccine based on codon harmonized full length Pfs48/45 expressed in Escherichia coli
    Debabani Roy Chowdhury
    Department of Molecular Microbiology and Immunology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD, USA
    PLoS ONE 4:e6352. 2009
    ....
  17. pmc Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies
    Elke S Bergmann-Leitner
    US Military Malaria Vaccine Program, Division of Malaria Vaccine Development, WRAIR, Silver Spring, MD, USA
    Malar J 7:129. 2008
    ..Purification of immunoglobulin is a standard approach for reducing such non-specific background activities, but the purification method itself can alter the quality and yield of recovered Ag-specific antibodies...
  18. doi request reprint Molecular adjuvants for malaria DNA vaccines based on the modulation of host-cell apoptosis
    Elke S Bergmann-Leitner
    Department of Molecular Parasitology, US Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Vaccine 27:5700-8. 2009
    ..This finding represents yet another example that strategies to enhance vaccine efficacy developed for other model systems such as viral diseases cannot easily be applied to any vaccine...
  19. pmc Impact of pre-existing MSP1(42)-allele specific immunity on potency of an erythrocytic Plasmodium falciparum vaccine
    Elke S Bergmann-Leitner
    Malaria Vaccine Branch, US Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Malar J 11:315. 2012
    ..The purpose of this study was to model the effect of pre-existing immunity to MSP142 on the immunogenicity of blood-stage malaria vaccines based on alternative MSP1 alleles...
  20. pmc MSP-1p42-specific antibodies affect growth and development of intra-erythrocytic parasites of Plasmodium falciparum
    Elke S Bergmann-Leitner
    US Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Malar J 8:183. 2009
    ..Understanding the mechanisms by which vaccine-induced anti-blood stage antibodies work in protecting against malaria is essential for vaccine design and testing...
  21. ncbi request reprint Miniaturization of a high-throughput pLDH-based Plasmodium falciparum growth inhibition assay for small volume samples from preclinical and clinical vaccine trials
    Elke S Bergmann-Leitner
    US Military Malaria Vaccine Program, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA
    Am J Trop Med Hyg 78:468-71. 2008
    ..This culture method can be extended beyond the pLDH-based readout to other techniques commonly used to determine growth/invasion inhibition...
  22. pmc DNA prime/Adenovirus boost malaria vaccine encoding P. falciparum CSP and AMA1 induces sterile protection associated with cell-mediated immunity
    Ilin Chuang
    US Military Malaria Vaccine Program, Naval Medical Research Center, Silver Spring, Maryland, United States of America
    PLoS ONE 8:e55571. 2013
    ..We tested a DNA prime/adenovirus boost malaria vaccine in a Phase 1 clinical trial with controlled human malaria infection...
  23. ncbi request reprint Molecular vaccines for malaria
    Joseph T Bruder
    GenVec, Inc, Gaithersburg, MD, USA
    Hum Vaccin 6:54-77. 2010
    ..In this review, we discuss the molecular aspects of antigen design, adjuvant advancement and the development of vaccine delivery systems as they are being applied to malaria vaccines...
  24. ncbi request reprint Characterization of a human reference standard for antibody to Plasmodium falciparum merozoite surface protein 1(42)
    In Kyu Yoon
    Division of Communicable Diseases and Immunology, Department of Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 72:714-8. 2005
    ..3 microg/mL of MSP-1(42) antibody. This reference standard may be useful as a quantitative working standard for measuring MSP-1(42) antibody response in future vaccine clinical trials involving MSP-1...
  25. pmc Development of a chimeric Plasmodium berghei strain expressing the repeat region of the P. vivax circumsporozoite protein for in vivo evaluation of vaccine efficacy
    Diego A Espinosa
    Johns Hopkins Malaria Research Institute and Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA
    Infect Immun 81:2882-7. 2013
    ..The chimeric parasites we developed represent a robust model for evaluating protective immune responses against P. vivax vaccines based on CSP. ..
  26. ncbi request reprint Towards an RTS,S-based, multi-stage, multi-antigen vaccine against falciparum malaria: progress at the Walter Reed Army Institute of Research
    D Gray Heppner
    Walter Reed Army Institute of Research, Department of Immunology, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Vaccine 23:2243-50. 2005
    ..Recent safety, immunogenicity, and efficacy trials in the US and Africa are presented, as well as plans for the development of a multi-antigen vaccine...
  27. ncbi request reprint Development and pre-clinical analysis of a Plasmodium falciparum Merozoite Surface Protein-1(42) malaria vaccine
    Evelina Angov
    Department of Immunology, WRAIR, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Mol Biochem Parasitol 128:195-204. 2003
    ..FMP1 formulated with GlaxoSmithKline Biologicals (GSK) adjuvant, AS02A or alum was safe and immunogenic in rhesus (Macaca mulatta) monkeys...
  28. pmc Activation of innate immunity in healthy Macaca mulatta macaques by a single subcutaneous dose of GMP CpG 7909: safety data and interferon-inducible protein-10 kinetics for humans and macaques
    V Ann Stewart
    Walter Reed Army Institute of Research, Silver Spring, Maryland, USA
    Clin Vaccine Immunol 15:221-6. 2008
    ....
  29. ncbi request reprint C3d binding to the circumsporozoite protein carboxy-terminus deviates immunity against malaria
    Elke S Bergmann-Leitner
    Department of Immunology, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Int Immunol 17:245-55. 2005
    ..We have thus identified C3d-mediated epitope masking and shifting of both the humoral and cellular immune responses as a potential novel escape mechanism, which plasmodia may use to divert the induction of protective immunity...
  30. pmc Computational and experimental validation of B and T-cell epitopes of the in vivo immune response to a novel malarial antigen
    Elke S Bergmann-Leitner
    Malaria Vaccine Branch, U S Military Malaria Vaccine Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
    PLoS ONE 8:e71610. 2013
    ..The limited accuracy of the tested algorithms to predict the in vivo immune responses emphasizes the need to improve their predictive capabilities for use as tools in vaccine design. ..
  31. pmc Transcutaneous immunization using colonization factor and heat-labile enterotoxin induces correlates of protective immunity for enterotoxigenic Escherichia coli
    Jianmei Yu
    Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA
    Infect Immun 70:1056-68. 2002
    ..Overall, our results demonstrate that relevant colonization factor and antitoxin immunity can be induced by TCI and suggest that an ETEC traveler's diarrhea vaccine could be delivered by using a patch...
  32. pmc The clinical-grade 42-kilodalton fragment of merozoite surface protein 1 of Plasmodium falciparum strain FVO expressed in Escherichia coli protects Aotus nancymai against challenge with homologous erythrocytic-stage parasites
    Christian A Darko
    Department of Immunology, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    Infect Immun 73:287-97. 2005
    ..Soluble MSP1(42) (FVO) expressed in E. coli offers excellent promise as a component of a vaccine against erythrocytic-stage falciparum malaria...
  33. pmc Enzyme-linked immunosorbent assay for detection of Plasmodium falciparum histidine-rich protein 2 in blood, plasma, and serum
    Carolyne M Kifude
    Walter Reed Project Kenya Medical Research Institute, Kisumu, Kenya
    Clin Vaccine Immunol 15:1012-8. 2008
    ..We conclude that PfHRP2 ELISA using whole-blood and serum samples is a suitable adjunct to microscopy and could ultimately benefit malaria intervention trials...
  34. pmc Safety and immunogenicity of an AMA-1 malaria vaccine in Malian adults: results of a phase 1 randomized controlled trial
    Mahamadou A Thera
    Malaria Research and Training Center, University of Bamako, Bamako, Mali
    PLoS ONE 3:e1465. 2008
    ..The objective was to evaluate the safety, reactogenicity and immunogenicity of the AMA-1-based blood-stage malaria vaccine FMP2.1/AS02A in adults exposed to seasonal malaria...
  35. ncbi request reprint Phase 1 randomized double-blind safety and immunogenicity trial of Plasmodium falciparum malaria merozoite surface protein FMP1 vaccine, adjuvanted with AS02A, in adults in western Kenya
    Jose A Stoute
    US Army Medical Research Unit and the Kenya Medical Research Institute, Nairobi, Kenya
    Vaccine 25:176-84. 2007
    ..16; P<0.001) and the Day 90 responses to MSP-1(42) (F1,335=16.69; P<0.001). The FMP1/AS02A vaccine is safe and immunogenic in adults and should progress to safety testing in children at greatest risk of malaria...
  36. ncbi request reprint Pre-clinical evaluation of the malaria vaccine candidate P. falciparum MSP1(42) formulated with novel adjuvants or with alum
    Sathit Pichyangkul
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Science AFRIMS, Bangkok, Thailand
    Vaccine 22:3831-40. 2004
    ..The notable difference in Th1/Th2 polarization induced by the AS02A and AS01B formulations warrants comparative clinical testing...
  37. ncbi request reprint Suramin and suramin analogues inhibit merozoite surface protein-1 secondary processing and erythrocyte invasion by the malaria parasite Plasmodium falciparum
    Suzanne L Fleck
    Medical Research Council Technology, 1 3 Burtonhole Lane, Mill Hill, London NW7 1AD, United Kingdom
    J Biol Chem 278:47670-7. 2003
    ..The results indicate that enzymatic events during invasion are suitable targets for drug development and validate the novel concept of an inhibitor binding to a macromolecular substrate to prevent its proteolysis by a protease...