Liqing Yu

Summary

Affiliation: Wake Forest University School of Medicine
Country: USA

Publications

  1. pmc Overexpression of ABCG5 and ABCG8 promotes biliary cholesterol secretion and reduces fractional absorption of dietary cholesterol
    Liqing Yu
    Department of Molecular Genetics and McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9046, USA
    J Clin Invest 110:671-80. 2002
  2. ncbi request reprint The structure and function of Niemann-Pick C1-like 1 protein
    Liqing Yu
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1040, USA
    Curr Opin Lipidol 19:263-9. 2008
  3. ncbi request reprint Cholesterol-regulated translocation of NPC1L1 to the cell surface facilitates free cholesterol uptake
    Liqing Yu
    Department of Pathology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    J Biol Chem 281:6616-24. 2006
  4. pmc Diosgenin stimulation of fecal cholesterol excretion in mice is not NPC1L1 dependent
    Ryan E Temel
    Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 50:915-23. 2009
  5. pmc Hepatic Niemann-Pick C1-like 1 regulates biliary cholesterol concentration and is a target of ezetimibe
    Ryan E Temel
    Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157 1040, USA
    J Clin Invest 117:1968-78. 2007
  6. pmc Niemann-pick C1-like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport
    Lin Jia
    Section on Lipid Sciences, Department of Pathology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    Annu Rev Physiol 73:239-59. 2011
  7. pmc Ezetimibe inhibits hepatic Niemann-Pick C1-Like 1 to facilitate macrophage reverse cholesterol transport in mice
    Ping Xie
    Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC, USA
    Arterioscler Thromb Vasc Biol 33:920-5. 2013
  8. pmc Biliary sterol secretion is not required for macrophage reverse cholesterol transport
    Ryan E Temel
    Section on Lipid Sciences, Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Cell Metab 12:96-102. 2010
  9. pmc CGI-58 knockdown in mice causes hepatic steatosis but prevents diet-induced obesity and glucose intolerance
    J Mark Brown
    Departments of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Lipid Res 51:3306-15. 2010
  10. pmc Deficiency of liver Comparative Gene Identification-58 causes steatohepatitis and fibrosis in mice
    Feng Guo
    Departments of Biochemistry and Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 54:2109-20. 2013

Research Grants

  1. NPC1L1 and Metabolic Diseases
    Liqing Yu; Fiscal Year: 2010

Collaborators

Detail Information

Publications32

  1. pmc Overexpression of ABCG5 and ABCG8 promotes biliary cholesterol secretion and reduces fractional absorption of dietary cholesterol
    Liqing Yu
    Department of Molecular Genetics and McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9046, USA
    J Clin Invest 110:671-80. 2002
    ....
  2. ncbi request reprint The structure and function of Niemann-Pick C1-like 1 protein
    Liqing Yu
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1040, USA
    Curr Opin Lipidol 19:263-9. 2008
    ..This review summarizes recent studies on NPC1L1 function and proposes a model for NPC1L1-dependent cholesterol uptake...
  3. ncbi request reprint Cholesterol-regulated translocation of NPC1L1 to the cell surface facilitates free cholesterol uptake
    Liqing Yu
    Department of Pathology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    J Biol Chem 281:6616-24. 2006
    ..These findings define a cholesterol-regulated endocytic recycling of NPC1L1 as a novel mechanism regulating cellular cholesterol uptake...
  4. pmc Diosgenin stimulation of fecal cholesterol excretion in mice is not NPC1L1 dependent
    Ryan E Temel
    Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 50:915-23. 2009
    ..In an in vitro assay, diosgenin was unable to block NPC1L1-dependent cholesterol uptake. In conclusion, diosgenin stimulation of fecal cholesterol excretion is independent of NPC1L1-mediated cholesterol absorption...
  5. pmc Hepatic Niemann-Pick C1-like 1 regulates biliary cholesterol concentration and is a target of ezetimibe
    Ryan E Temel
    Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157 1040, USA
    J Clin Invest 117:1968-78. 2007
    ..These findings suggest that in humans, ezetimibe may reduce plasma cholesterol by inhibiting NPC1L1 function in both intestine and liver, and hepatic NPC1L1 may have evolved to protect the body from excessive biliary loss of cholesterol...
  6. pmc Niemann-pick C1-like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport
    Lin Jia
    Section on Lipid Sciences, Department of Pathology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1040, USA
    Annu Rev Physiol 73:239-59. 2011
    ..Future studies should focus on molecular mechanisms underlying NPC1L1-dependent cholesterol transport and elucidation of how a cholesterol transporter modulates the pathogenesis of metabolic diseases...
  7. pmc Ezetimibe inhibits hepatic Niemann-Pick C1-Like 1 to facilitate macrophage reverse cholesterol transport in mice
    Ping Xie
    Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC, USA
    Arterioscler Thromb Vasc Biol 33:920-5. 2013
    ....
  8. pmc Biliary sterol secretion is not required for macrophage reverse cholesterol transport
    Ryan E Temel
    Section on Lipid Sciences, Department of Pathology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Cell Metab 12:96-102. 2010
    ..Collectively, these studies demonstrate that mass fecal sterol loss and macrophage RCT can proceed in the absence of biliary sterol secretion, challenging the obligate role of bile in RCT...
  9. pmc CGI-58 knockdown in mice causes hepatic steatosis but prevents diet-induced obesity and glucose intolerance
    J Mark Brown
    Departments of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Lipid Res 51:3306-15. 2010
    ....
  10. pmc Deficiency of liver Comparative Gene Identification-58 causes steatohepatitis and fibrosis in mice
    Feng Guo
    Departments of Biochemistry and Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 54:2109-20. 2013
    ..In conclusion, CGI-58 deficiency in the liver directly causes not only hepatic steatosis but also steatohepatitis and fibrosis. ..
  11. pmc Protein mediators of sterol transport across intestinal brush border membrane
    J Mark Brown
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC, USA
    Subcell Biochem 51:337-80. 2010
    ..The purpose of this work is to summarize substantial progress made in the last decade in regards to protein-mediated sterol trafficking, and to discuss this in the context of human disease...
  12. ncbi request reprint Niemann-Pick C1-like 1 is required for an LXR agonist to raise plasma HDL cholesterol in mice
    Weiqing Tang
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1040, USA
    Arterioscler Thromb Vasc Biol 28:448-54. 2008
    ..Because cholesterol is essential for HDL assembly and directly regulates intestinal ABCA1 expression via activating LXR, we hypothesized that cholesterol absorption, a major function of intestine, modulates LXR-dependent HDL formation...
  13. pmc Niemann-Pick C1-Like 1 deletion in mice prevents high-fat diet-induced fatty liver by reducing lipogenesis
    Lin Jia
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1040, USA
    J Lipid Res 51:3135-44. 2010
    ....
  14. pmc The full capacity of AICAR to reduce obesity-induced inflammation and insulin resistance requires myeloid SIRT1
    Zhenggang Yang
    Department of Internal Medicine, Wake Forest University School of Medicine, Winston Salem, NC, USA
    PLoS ONE 7:e49935. 2012
    ..Myeloid SIRT1 is a therapeutic target of the anti-inflammatory and insulin-sensitizing effects of AICAR...
  15. pmc Dietary cholesterol reverses resistance to diet-induced weight gain in mice lacking Niemann-Pick C1-Like 1
    Lin Jia
    Department of Pathology and Biochemistry, Wake Forest University School of Medicine, Winston Salem, NC, USA
    J Lipid Res 51:3024-33. 2010
    ..Under all diets, L1-KO mice were protected from hepatosteatosis. In conclusion, increasing dietary cholesterol restores diet-induced weight gain in mice deficient in NPC1L1-dependent cholesterol absorption...
  16. pmc NPC1L1 (Niemann-Pick C1-like 1) mediates sterol-specific unidirectional transport of non-esterified cholesterol in McArdle-RH7777 hepatoma cells
    J Mark Brown
    Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1040, U S A
    Biochem J 406:273-83. 2007
    ..Collectively, these findings support the notion that NPC1L1 can selectively recognize non-esterified cholesterol and promote its unidirectional transport into hepatoma cells...
  17. ncbi request reprint Plasma cholesterol is hyperresponsive to statin in ABCG5/ABCG8 transgenic mice
    Weiqing Tang
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Hepatology 44:1259-66. 2006
    ..A synergistic hypocholesterolemic effect could be potentially achieved in humans by simultaneously inhibiting cholesterol biosynthesis and promoting ABCG5/ABCG8-mediated cholesterol excretion...
  18. ncbi request reprint CGI-58 facilitates the mobilization of cytoplasmic triglyceride for lipoprotein secretion in hepatoma cells
    J Mark Brown
    Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157 1040, USA
    J Lipid Res 48:2295-305. 2007
    ..Collectively, this study reveals a role for CGI-58 in coupling lipolytic degradation of cytoplasmic TG to oxidation and packaging into TG-rich lipoproteins for secretion in hepatoma cells...
  19. pmc NPC1L1 and cholesterol transport
    Jenna L Betters
    Department of Pathology Section on Lipid Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157 1040, USA
    FEBS Lett 584:2740-7. 2010
    ..Recently, NPC1L1 inhibition has been shown to have beneficial effects on components of the metabolic syndrome, such as obesity, insulin resistance, and fatty liver, in addition to atherosclerosis...
  20. pmc Epigenetic regulation of macrophage polarization by DNA methyltransferase 3b
    Xiaosong Yang
    Center for Infection and Immunity Research X Y, D L, School of Life Sciences, Hubei University, Wuhan, China Department of Biology X Y, B X, H S, Georgia State University, Atlanta, Georgia 30303 Department of Internal Medicine X W, B X, H S, Wake Forest University School of Medicine, Winston Salem, North Carolina 27106 and Department of Animal and Avian Sciences L Y, University of Maryland, College Park, Maryland 20742
    Mol Endocrinol 28:565-74. 2014
    ..In obesity, elevated saturated fatty acids enhance DNMT3b expression, leading to DNA methylation at the PPARγ1 promoter, which may contribute to deregulated adipose tissue macrophage polarization, inflammation, and insulin resistance. ..
  21. pmc Regulation of insulin and leptin signaling by muscle suppressor of cytokine signaling 3 (SOCS3)
    Zhenggang Yang
    Department of Internal Medicine, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    PLoS ONE 7:e47493. 2012
    ..These studies demonstrate that SOC3 within skeletal muscle is a critical regulator of leptin and insulin action and that increased SOCS may mediate insulin and leptin resistance in obesity...
  22. ncbi request reprint Kinetic imaging of NPC1L1 and sterol trafficking between plasma membrane and recycling endosomes in hepatoma cells
    Nicole Hartwig Petersen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, Denmark
    J Lipid Res 49:2023-37. 2008
    ..Our study demonstrates dynamic trafficking of NPC1L1 between the cell surface and intracellular compartments and suggests that this transport is involved in NPC1L1-mediated cellular sterol uptake...
  23. pmc Disruption of Abcg5 and Abcg8 in mice reveals their crucial role in biliary cholesterol secretion
    Liqing Yu
    McDermott Center for Human Growth and Development and Departments of Molecular Genetics and Biochemistry, The Howard Hughes Medical Institute and Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Proc Natl Acad Sci U S A 99:16237-42. 2002
    ....
  24. ncbi request reprint Stimulation of cholesterol excretion by the liver X receptor agonist requires ATP-binding cassette transporters G5 and G8
    Liqing Yu
    McDermott Center for Human Growth and Development, Departments of Molecular Genetics and Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9046, USA
    J Biol Chem 278:15565-70. 2003
    ..Thus Abcg5 and Abcg8 are required for LXR agonist-associated changes in dietary and biliary sterol trafficking. These results establish a central role for ABCG5 and ABCG8 in promoting cholesterol excretion in vivo...
  25. ncbi request reprint ABCG5 and ABCG8 are obligate heterodimers for protein trafficking and biliary cholesterol excretion
    Gregory A Graf
    McDermott Center for Human Growth and Development and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, 75390, USA
    J Biol Chem 278:48275-82. 2003
    ..Finally, adenoviral expression of G2 in the presence or absence of G5 or G8 failed to promote sterol excretion into bile. These experiments indicate that G5 and G8 function as obligate heterodimers to promote sterol excretion into bile...
  26. ncbi request reprint Selective sterol accumulation in ABCG5/ABCG8-deficient mice
    Liqing Yu
    McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    J Lipid Res 45:301-7. 2004
    ..Thus, the accumulation of sterols other than cholesterol is sensed by the cholesterol regulatory machinery...
  27. ncbi request reprint Ezetimibe normalizes metabolic defects in mice lacking ABCG5 and ABCG8
    Liqing Yu
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 46:1739-44. 2005
    ..Together, these results indicate that pharmacological blockade of sterol absorption can ameliorate the deleterious metabolic effects of plant sterols even in the absence of G5 and G8...
  28. ncbi request reprint High-level expression of ABCG5 and ABCG8 attenuates diet-induced hypercholesterolemia and atherosclerosis in Ldlr-/- mice
    Kenneth R Wilund
    Departments of Molecular Genetics and Internal Medicine, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75290, USA
    J Lipid Res 45:1429-36. 2004
    ..These results demonstrate that increased expression of G5 and G8 attenuates diet-induced hypercholesterolemia in Ldlr-/- mice, resulting in a significant reduction in plasma levels of cholesterol and aortic atherosclerotic lesion area...
  29. pmc Disruption of cholesterol homeostasis by plant sterols
    Chendong Yang
    Department of Molecular Genetics, McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas 75390 9046, USA
    J Clin Invest 114:813-22. 2004
    ..Stigmasterol also activated the liver X receptor in a cell-based reporter assay. These data indicate that selected dietary plant sterols disrupt cholesterol homeostasis by affecting two critical regulatory pathways of lipid metabolism...
  30. ncbi request reprint Expression of ABCG5 and ABCG8 is required for regulation of biliary cholesterol secretion
    Liqing Yu
    McDermott Center for Human Growth and Development, Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 280:8742-7. 2005
    ..Thus G5 and G8 are required to modulate biliary cholesterol secretion in response to cholate and diosgenin, but the choleretic effects of these two steroids are mediated by different mechanisms requiring FXR and PXR, respectively...
  31. ncbi request reprint ABCG5 and ABCG8 require MDR2 for secretion of cholesterol into bile
    Silvia Langheim
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Lipid Res 46:1732-8. 2005
    ..These results are consistent with the notion that increased biliary cholesterol secretion contributes to the reduction in fractional sterol absorption associated with G5G8 overexpression...
  32. ncbi request reprint Sterol regulation of scavenger receptor class B type I in macrophages
    Liqing Yu
    Department of Molecular Genetics and McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    J Lipid Res 45:889-99. 2004
    ..We conclude that SR-BI levels in macrophages are responsive to changes in intracellular sterol content and that these sterol-associated changes are not mediated by LXR and are unlikely to be mediated by an SREBP pathway...

Research Grants1

  1. NPC1L1 and Metabolic Diseases
    Liqing Yu; Fiscal Year: 2010
    ..Given that NPC1L1 is the target of ezetimibe, an FDA-approved intestinal cholesterol absorption inhibitor that is widely used to lower blood cholesterol in humans, these studies have enormous translational potential. ..