Research Topics
Genomes and GenesSpecies | Kent E VranaSummaryAffiliation: Wake Forest University School of Medicine Country: USA Publications
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Detail Information
Publications
Embryonic stem cells from parthenotesJose B Cibelli
Department of Animal Science, Michigan State University, East Lansing, Michigan, USA
Methods Enzymol 418:117-35. 2006..This chapter describes the biology underlying parthenogenesis, as well as provides detailed technical considerations for the production of pESCs...- Nuclear proteomics and directed differentiation of embryonic stem cellsMiguel Barthelery
Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
Stem Cells Dev 16:905-19. 2007.... - Use of microarray technologies in toxicology researchKent E Vrana
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, 27157 1083, Winston Salem, NC, USA
Neurotoxicology 24:321-32. 2003.... 
Nonhuman primate parthenogenetic stem cellsKent E Vrana
Center for Neurobehavioral Study of Alcohol, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
Proc Natl Acad Sci U S A 100:11911-6. 2003..These results suggest that stem cells derived from the parthenogenetically activated nonhuman primate egg provide a potential source for autologous cell therapy in the female and bypass the need for creating a competent embryo...- Extended cocaine self-administration and deprivation produces region-specific and time-dependent changes in connexin36 expression in rat brainClinton B McCracken
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
Synapse 58:141-50. 2005..These results suggest that changes in neuronal gap junction expression may be one mechanism by which cocaine self-administration produces enduring changes in behavior... - Manganese-induced cytotoxicity in dopamine-producing cellsDenise L Stredrick
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1083, USA
Neurotoxicology 25:543-53. 2004..In conclusion, DA content was not responsible for the enhanced sensitivity of CATH.a cells to Mn toxicity, but oxidative stress was implicated as a probable mechanism of cytotoxicity... - Systematic screening of gene expression using a cDNA macroarrayTravis J Worst
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC, USA
Methods Mol Med 79:243-59. 2003 - Repeated cocaine self-administration causes multiple changes in rat frontal cortex gene expressionWillard M Freeman
Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem NC 27157 1083, USA
Neurochem Res 27:1181-92. 2002..These changes share commonalities and exhibit differences with previous reports of gene expression changes in the frontal cortex after noncontingent cocaine administration... - Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring ratsTravis J Worst
Center for the Neurobehavioral Study of Alcohol, Department of Physiology and Pharmacology, Wake Forest University Health Sciences, Winston Salem, North Carolina, USA
J Neurosci Res 80:529-38. 2005..Such alterations, however, might not be a universal characteristic of all animal models of alcohol abuse and will also require further investigation in post-mortem human samples... - An interactive database of cocaine-responsive gene expressionWillard M Freeman
Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1083, USA
ScientificWorldJournal 2:701-6. 2002..Furthermore, this method of organizing large volumes of scientific information can easily be adapted to assist researchers in fields outside of drug abuse... - Alcohol and gene expression in the central nervous systemTravis J Worst
Center for the Neurobehavioral Study of Alcohol, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA
Alcohol Alcohol 40:63-75. 2005.... - Functional genomic analysis in pain research using hybridization arraysStephen J Walker
Center for the Neurobiological Investigation of Drug Abuse and Neurobehavioral Study of Alcohol, Wake Forest University School of Medicine, Winston-Salem, NC, USA
Methods Mol Med 99:239-53. 2004..The experimental description in this chapter explains, in detail, how to perform a hybridization array using the macroarray platform... - Semiquantitative real-time PCR for analysis of mRNA levelsStephen J Walker
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC, USA
Methods Mol Med 79:211-27. 2003 - Induction of GADD45 and GADD153 in neuroblastoma cells by dopamine-induced toxicityAlan H Stokes
Department of Physiology and Pharmacology, Center for Neurobiological Investigation of Drug Abuse, Wake Forest University School of Medicine, Winston-Salem, NC 27157-1083, USA
Neurotoxicology 23:675-84. 2002..Other genes that displayed changes, but that have not been subjected to post-hoc confirmation, include clusterin (increased), ubiquitin (increased), CD27 ligand (increased), CD27BP (increased), and rac-PK-beta (decreased)... - Amphetamine withdrawal produces region-specific and time-dependent changes in connexin36 expression in rat brainClinton B McCracken
Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA
Synapse 56:39-44. 2005..This is, to our knowledge, the first demonstration of pharmacological manipulation of connexin36 in vivo... - Effects of manganese (Mn) on the developing rat brain: oxidative-stress related endpointsSarah Weber
Department of Physiology and Pharmacology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27157-1083, USA
Neurotoxicology 23:169-75. 2002..05) total cerebrocortical GSH without accompanying changes in any of the other measured parameters. Therefore, it is unlikely that high dose Mn exposure is associated with oxidative stress in this experimental paradigm... - Changes in rat frontal cortex gene expression following chronic cocaineWillard M Freeman
Center for the Neurobiological Investigation of Drug Abuse, Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1083, USA
Brain Res Mol Brain Res 104:11-20. 2002..This study also validates the use of hybridization arrays for screening of neuronal gene expression changes and the utility of relative protein quantification as a post-hoc confirmation tool... - Parthenogenetic stem cells in nonhuman primatesJose B Cibelli
Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA
Science 295:819. 2002 - Glucocorticoids plus opioids up-regulate genes that influence neuronal functionGregg R Ward
Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
Cell Mol Neurobiol 27:651-60. 2007..Future studies are warranted to determine if combined influences of glucocorticoid fluctuations and opioid receptor stimulation in vivo can orchestrate exagerated neuroadaptation to reinforcing drugs under chronic mild stress conditions... 
Enhanced nuclear proteomicsMiguel Barthelery
Department of Pharmacology, The Pennsylvania State University College of Medicine, PA, USA
Proteomics 8:1832-8. 2008..This technique greatly improves electrofocusing efficacy and nearly doubles the number of detected protein spots. This approach to nuclear protein isolation for 2-D PAGE opens the door to better investigation of nuclear protein dynamics...- Depletion of abundant proteins from non-human primate serum for biomarker studiesWillard M Freeman
Department of Pharmacology H078, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
Proteomics 6:3109-13. 2006..Depleted serum also demonstrated greater sensitivity for previously masked, lower-abundance proteins... - Apo-AII is an elevated biomarker of chronic non-human primate ethanol self-administrationWillard M Freeman
Department of Pharmacology, H078, Penn State College of Medicine, 500 University Drive, PO Box 850, Hershey, PA 17033 0850, USA
Alcohol Alcohol 41:300-5. 2006.... - Analysis of cellular, transgenic and human models of Huntington's disease reveals tyrosine hydroxylase alterations and substantia nigra neuropathologyGeorge J Yohrling
Department of Neurology, Center for Aging, Genetics, and Neurodegeneration, Massachusetts General Hospital, 114 16th Street, B114-2000, Charlestown, MA 02129-4404, USA
Brain Res Mol Brain Res 119:28-36. 2003..These findings implicate abnormalities in dopamine neurotransmission in HD and may provide new insights into targets for pharmacotherapy... - Emerging techniques in biomedical research and their application to alcohol toxicityVinood B Patel
Department of Biochemistry and the Center for the Neurobehavioral Study of Alcohol, North Carolina, USA
Alcohol Clin Exp Res 27:348-53. 2003..Vrana; and (5) Adeno- and adeno-associated viral mediated gene transfer approaches for alcoholic liver disease, by Michael Wheeler. Concluding remarks were by Victor R. Preedy... - Inhibition of tryptophan hydroxylase activity and decreased 5-HT1A receptor binding in a mouse model of Huntington's diseaseGeorge J Yohrling IV
Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
J Neurochem 82:1416-23. 2002..The presymptomatic inhibition of TPH activity in the R6/2 mice may help explain the functional consequences of HD and provide insights into new targets for pharmacotherapy... - A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitopeJohn W Haycock
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, LSUHSC BIOCHEM, 1100 Florida Avenue, New Orleans, LA 70119, USA
J Neurosci Methods 114:205-12. 2002.... - Heroin self-administration: II. CNS gene expression following withdrawal and cue-induced drug-seeking behaviorKARA L KUNTZ
Department of Pharmacology, Pennsylvania State University College of Medicine, R130, 500 University Drive, Hershey, PA 17033, USA
Pharmacol Biochem Behav 90:349-56. 2008..These latter observations of persistent changes in gene expression following abstinence may reflect molecular correlates of relapse liability...