SEAN REID

Summary

Affiliation: Wake Forest University School of Medicine
Country: USA

Publications

  1. pmc Srv mediated dispersal of streptococcal biofilms through SpeB is observed in CovRS+ strains
    Kristie L Connolly
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina, United States of America
    PLoS ONE 6:e28640. 2011
  2. pmc Dispersal of Group A streptococcal biofilms by the cysteine protease SpeB leads to increased disease severity in a murine model
    Kristie L Connolly
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina, United States of America
    PLoS ONE 6:e18984. 2011
  3. pmc The streptococcal collagen-like protein-1 (Scl1) is a significant determinant for biofilm formation by group A Streptococcus
    Heaven A Oliver-Kozup
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506, USA
    BMC Microbiol 11:262. 2011
  4. pmc Detection of group A Streptococcus in tonsils from pediatric patients reveals high rate of asymptomatic streptococcal carriage
    Amity L Roberts
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC, USA
    BMC Pediatr 12:3. 2012
  5. pmc Characterization of an extracellular virulence factor made by group A Streptococcus with homology to the Listeria monocytogenes internalin family of proteins
    Sean D Reid
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 71:7043-52. 2003
  6. pmc Identification of srv, a PrfA-like regulator of group A streptococcus that influences virulence
    Sean D Reid
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 72:1799-803. 2004
  7. pmc Inactivation of the group A Streptococcus regulator srv results in chromosome wide reduction of transcript levels, and changes in extracellular levels of Sic and SpeB
    Sean D Reid
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    FEMS Immunol Med Microbiol 48:283-92. 2006
  8. pmc Allelic replacement of the streptococcal cysteine protease SpeB in a Δsrv mutant background restores biofilm formation
    Amity L Roberts
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina, 27157, USA
    BMC Res Notes 3:281. 2010
  9. pmc Opsonophagocytosis-inhibiting mac protein of group a streptococcus: identification and characteristics of two genetic complexes
    Benfang Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:6880-90. 2002
  10. pmc Identification and characterization of a novel heme-associated cell surface protein made by Streptococcus pyogenes
    Benfang Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:4494-500. 2002

Collaborators

Detail Information

Publications19

  1. pmc Srv mediated dispersal of streptococcal biofilms through SpeB is observed in CovRS+ strains
    Kristie L Connolly
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina, United States of America
    PLoS ONE 6:e28640. 2011
    ..This work provides additional evidence that Srv regulated control of SpeB may mediate biofilm formation and dispersal in diverse strain backgrounds...
  2. pmc Dispersal of Group A streptococcal biofilms by the cysteine protease SpeB leads to increased disease severity in a murine model
    Kristie L Connolly
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina, United States of America
    PLoS ONE 6:e18984. 2011
    ..Taken together, these data suggest a mechanism by which GAS disease may transition from mild to severe through the Srv mediated dispersal of GAS biofilms...
  3. pmc The streptococcal collagen-like protein-1 (Scl1) is a significant determinant for biofilm formation by group A Streptococcus
    Heaven A Oliver-Kozup
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506, USA
    BMC Microbiol 11:262. 2011
    ..In this study, we demonstrate that surface-associated streptococcal collagen-like protein-1 (Scl1) plays an important role in GAS biofilm formation...
  4. pmc Detection of group A Streptococcus in tonsils from pediatric patients reveals high rate of asymptomatic streptococcal carriage
    Amity L Roberts
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC, USA
    BMC Pediatr 12:3. 2012
    ..The objective of this study was to test the hypothesis that GAS is present within tonsil tissue at the time of tonsillectomy...
  5. pmc Characterization of an extracellular virulence factor made by group A Streptococcus with homology to the Listeria monocytogenes internalin family of proteins
    Sean D Reid
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 71:7043-52. 2003
    ..These studies characterize the first GAS LRR protein as an extracellular virulence factor that contributes to pathogenesis and may participate in evasion of the innate host defense...
  6. pmc Identification of srv, a PrfA-like regulator of group A streptococcus that influences virulence
    Sean D Reid
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 72:1799-803. 2004
    ..The gene, named srv for streptococcal regulator of virulence, encodes a 240-amino-acid protein with 53% amino acid similarity to PrfA, a transcriptional activator of virulence in Listeria monocytogenes...
  7. pmc Inactivation of the group A Streptococcus regulator srv results in chromosome wide reduction of transcript levels, and changes in extracellular levels of Sic and SpeB
    Sean D Reid
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    FEMS Immunol Med Microbiol 48:283-92. 2006
    ..Sic levels are reduced in the srv mutant, whereas the extracellular concentration and activity of SpeB is increased. These data link Srv to the increasingly complex GAS regulatory network...
  8. pmc Allelic replacement of the streptococcal cysteine protease SpeB in a Δsrv mutant background restores biofilm formation
    Amity L Roberts
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina, 27157, USA
    BMC Res Notes 3:281. 2010
    ..To address this question, we constructed a ΔsrvΔspeB double mutant through allelic replacement (MGAS5005ΔsrvΔspeB) and tested its ability to form biofilms in vitro...
  9. pmc Opsonophagocytosis-inhibiting mac protein of group a streptococcus: identification and characteristics of two genetic complexes
    Benfang Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:6880-90. 2002
    ..Taken together, the data add to the emerging theme in GAS pathogenesis that allelic variation in virulence genes contributes to fundamental differences in host-pathogen interactions among strains...
  10. pmc Identification and characterization of a novel heme-associated cell surface protein made by Streptococcus pyogenes
    Benfang Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:4494-500. 2002
    ..The shp gene was cotranscribed with eight contiguous genes, including homologues of an ABC transporter involved in iron uptake in gram-negative bacteria. Our results indicate that Shp is a novel cell surface heme-associated protein...
  11. pmc Postgenomic analysis of four novel antigens of group a streptococcus: growth phase-dependent gene transcription and human serologic response
    Sean D Reid
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 184:6316-24. 2002
    ..These results are additional evidence that postgenomic strategies provide new ways to identify and investigate novel bacterial proteins that may participate in host-pathogen interactions or serve as targets for therapeutics research...
  12. pmc Angiotensin-(1-7)-angiotensin-converting enzyme 2 attenuates reactive oxygen species formation to angiotensin II within the cell nucleus
    TanYa M Gwathmey
    Hypertension and Vascular Research Center, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA
    Hypertension 55:166-71. 2010
    ..We conclude that an ACE2-Ang-(1-7)-AT(7)R pathway modulates Ang II-dependent ROS formation within the nucleus, providing a unique protective mechanism against oxidative stress and cell damage...
  13. pmc Biofilm formation by group A Streptococcus: a role for the streptococcal regulator of virulence (Srv) and streptococcal cysteine protease (SpeB)
    Christopher D Doern
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Microbiology 155:46-52. 2009
    ..Complementation of MGAS5005Deltasrv restored the biofilm phenotype and eliminated the overproduction of active SpeB. Inhibition of SpeB with E64 also restored the MGAS5005Deltasrv biofilm to wild-type levels...
  14. pmc Loss of the group A Streptococcus regulator Srv decreases biofilm formation in vivo in an otitis media model of infection
    Amity L Roberts
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Infect Immun 78:4800-8. 2010
    ..Interestingly, our results suggest that GAS naturally forms a biofilm during otitis media but that biofilm formation is not required to establish infection following transbullar inoculation of chinchillas...
  15. pmc Point mutations within the streptococcal regulator of virulence (Srv) alter protein-DNA interactions and Srv function
    Christopher D Doern
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Microbiology 154:1998-2007. 2008
    ..Complementation of MGAS5005Deltasrv with one of these site-directed mutants failed to restore wild-type SpeB activity. Taken together, these data suggest that the Srv HTH is necessary for DNA binding and Srv function...
  16. pmc The type III pantothenate kinase encoded by coaX is essential for growth of Bacillus anthracis
    Carleitta Paige
    Center for Structural Biology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA
    J Bacteriol 190:6271-5. 2008
    ..We have demonstrated by analyzing the growth characteristics of a conditional coaX mutant that PanK(Ba) is an essential enzyme, thus contributing to its validation as a new antimicrobial target...
  17. pmc Pivotal Advance: Nonfunctional lung effectors exhibit decreased calcium mobilization associated with reduced expression of ORAI1
    Subhashini Arimilli
    1 Room 5140 Gray Building, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    J Leukoc Biol 87:977-88. 2010
    ..The reduced ability to mobilize calcium was associated with reduced expression of ORAI1, the CRAC channel subunit. These findings reveal a previously unknown mechanism for the negative regulation of function in effector T cells...
  18. pmc Genome diversification in Staphylococcus aureus: Molecular evolution of a highly variable chromosomal region encoding the Staphylococcal exotoxin-like family of proteins
    J Ross Fitzgerald
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 71:2827-38. 2003
    ..The coexpression of many set genes and the production of multiple SET proteins during human infection suggests an important role in host-pathogen interactions...
  19. ncbi request reprint Macrolide-resistant Streptococcus pyogenes in the United States, 2002-2003
    Sandra S Richter
    Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA 52242 1009, USA
    Clin Infect Dis 41:599-608. 2005
    ..pyogenes isolates from throughout the United States and to elucidate the mechanisms of resistance and genetic relatedness of macrolide-resistant isolates...

Research Grants4

  1. Srv is critical for Streptococcal survival in the host
    SEAN REID; Fiscal Year: 2006
    ..Understanding the mechanism of Srv-dependent gene regulation will provide new insights into the control of GAS disease. ..
  2. Srv is critical for Streptococcal survival in the host
    SEAN REID; Fiscal Year: 2007
    ..Understanding the mechanism of Srv-dependent gene regulation will provide new insights into the control of GAS disease. ..
  3. Srv is critical for Streptococcal survival in the host
    SEAN REID; Fiscal Year: 2009
    ..Understanding the mechanism of Srv-dependent gene regulation will provide new insights into the control of GAS disease. ..