STEVEN MIZEL

Summary

Affiliation: Wake Forest University School of Medicine
Country: USA

Publications

  1. ncbi Gram-negative flagellin-induced self-tolerance is associated with a block in interleukin-1 receptor-associated kinase release from toll-like receptor 5
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 277:22414-20. 2002
  2. ncbi Flagellin-F1-V fusion protein is an effective plague vaccine in mice and two species of nonhuman primates
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1064, USA
    Clin Vaccine Immunol 16:21-8. 2009
  3. ncbi Flagellin as an adjuvant: cellular mechanisms and potential
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 185:5677-82. 2010
  4. ncbi Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 170:6217-23. 2003
  5. ncbi Identification of a sequence in human toll-like receptor 5 required for the binding of Gram-negative flagellin
    Steven B Mizel
    Department of Microbiology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 278:23624-9. 2003
  6. ncbi Gangliosides inhibit flagellin signaling in the absence of an effect on flagellin binding to toll-like receptor 5
    A Phillip West
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 280:9482-8. 2005
  7. ncbi Flagellin is an effective adjuvant for immunization against lethal respiratory challenge with Yersinia pestis
    Anna N Honko
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, USA
    Infect Immun 74:1113-20. 2006
  8. ncbi Mucosal administration of flagellin induces innate immunity in the mouse lung
    Anna N Honko
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    Infect Immun 72:6676-9. 2004
  9. ncbi Mucosal adjuvant activity of flagellin in aged mice
    John T Bates
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Mech Ageing Dev 129:271-81. 2008
  10. ncbi Immunization of young African green monkeys with OprF epitope 8-OprI-type A- and B-flagellin fusion proteins promotes the production of protective antibodies against nonmucoid Pseudomonas aeruginosa
    Eric T Weimer
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Vaccine 27:6762-9. 2009

Research Grants

  1. TRAINING PROGRAM IN IMMUNOLOGY AND PATHOGENESIS
    STEVEN MIZEL; Fiscal Year: 2007
  2. Preclinical Development of Flagellin as a Mucosal Adjuvant in Biodefense Vaccines
    STEVEN MIZEL; Fiscal Year: 2007
  3. Activation of inflammatory cells by flagellin
    STEVEN MIZEL; Fiscal Year: 2006

Collaborators

Detail Information

Publications17

  1. ncbi Gram-negative flagellin-induced self-tolerance is associated with a block in interleukin-1 receptor-associated kinase release from toll-like receptor 5
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 277:22414-20. 2002
    ..LPS-induced cross-tolerance to flagellin is also associated with a block in IRAK release from TLR5. These results provide evidence for a novel mechanism of TLR signaling control...
  2. ncbi Flagellin-F1-V fusion protein is an effective plague vaccine in mice and two species of nonhuman primates
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1064, USA
    Clin Vaccine Immunol 16:21-8. 2009
    ..On the basis of our results, we believe that flagellin-F1-V is an outstanding candidate for evaluation in studies with humans...
  3. ncbi Flagellin as an adjuvant: cellular mechanisms and potential
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 185:5677-82. 2010
    ..This review summarizes the state of our current understanding of the adjuvant effect of flagellin and addresses important areas of current and future research interest...
  4. ncbi Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes
    Steven B Mizel
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 170:6217-23. 2003
    ....
  5. ncbi Identification of a sequence in human toll-like receptor 5 required for the binding of Gram-negative flagellin
    Steven B Mizel
    Department of Microbiology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 278:23624-9. 2003
    ..We also show that the NH2-terminal 358 amino acids of TLR5 play an important role in its signaling activity. Our results provide, for the first time, a molecular basis for the agonist specificity of a TLR...
  6. ncbi Gangliosides inhibit flagellin signaling in the absence of an effect on flagellin binding to toll-like receptor 5
    A Phillip West
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 280:9482-8. 2005
    ....
  7. ncbi Flagellin is an effective adjuvant for immunization against lethal respiratory challenge with Yersinia pestis
    Anna N Honko
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina 27157, USA
    Infect Immun 74:1113-20. 2006
    ..pestis CO92, with 93 to 100% survival of immunized mice. Lastly, vaccination of cynomolgus monkeys with flagellin and a fusion of the F1 and V antigens of Y. pestis induced a robust antigen-specific IgG antibody response...
  8. ncbi Mucosal administration of flagellin induces innate immunity in the mouse lung
    Anna N Honko
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    Infect Immun 72:6676-9. 2004
    ....
  9. ncbi Mucosal adjuvant activity of flagellin in aged mice
    John T Bates
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Mech Ageing Dev 129:271-81. 2008
    ..Our findings are consistent with the conclusion that flagellin can promote adaptive immune responses in aged mice, but at a less robust level than in young mice...
  10. ncbi Immunization of young African green monkeys with OprF epitope 8-OprI-type A- and B-flagellin fusion proteins promotes the production of protective antibodies against nonmucoid Pseudomonas aeruginosa
    Eric T Weimer
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Vaccine 27:6762-9. 2009
    ..Based on our results, OprF(311-341)-OprI-A- and B-flagellin fusion proteins are highly effective in mice and nonhuman primates and thus merit additional development as a potential vaccine for use in humans...
  11. ncbi Stimulation of human dendritic cells by wild-type and M protein mutant vesicular stomatitis viruses engineered to express bacterial flagellin
    Maryam Ahmed
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Virol 84:12093-8. 2010
    ..Therefore, rwt and rM51R-M viruses expressing flagellin may be promising vectors for the delivery of foreign antigen due to their potential to stimulate DC function...
  12. ncbi A fusion protein vaccine containing OprF epitope 8, OprI, and type A and B flagellins promotes enhanced clearance of nonmucoid Pseudomonas aeruginosa
    Eric T Weimer
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Infect Immun 77:2356-66. 2009
    ..aeruginosa, which appears to be the phenotype of the bacterium that initially colonizes CF patients...
  13. ncbi Direct stimulation of tlr5+/+ CD11c+ cells is necessary for the adjuvant activity of flagellin
    John T Bates
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Immunol 182:7539-47. 2009
    ....
  14. ncbi Protection against lethal vaccinia virus challenge by using an attenuated matrix protein mutant vesicular stomatitis virus vaccine vector expressing poxvirus antigens
    Cassandra L Braxton
    Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA
    J Virol 84:3552-61. 2010
    ..These results demonstrate the potential of M protein mutant VSVs as candidate vaccine vectors against human diseases...
  15. ncbi A recombinant flagellin-poxvirus fusion protein vaccine elicits complement-dependent protection against respiratory challenge with vaccinia virus in mice
    Kristen N Delaney
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Viral Immunol 23:201-10. 2010
    ..Our results are consistent with the conclusion that flagellin-L1R and flagellin-B5R fusion proteins are effective in eliciting protective immunity against vaccinia virus that is dependent, in large part, on complement...
  16. ncbi Engineered expression of the TLR5 ligand flagellin enhances paramyxovirus activation of human dendritic cell function
    Subhashini Arimilli
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157 1064, USA
    J Virol 82:10975-85. 2008
    ....
  17. ncbi Effects of flagellin on innate and adaptive immunity
    Anna N Honko
    Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA
    Immunol Res 33:83-101. 2005
    ..This review focuses on the mechanisms of action of flagellin and its effects on both innate and adaptive immunity...

Research Grants10

  1. TRAINING PROGRAM IN IMMUNOLOGY AND PATHOGENESIS
    STEVEN MIZEL; Fiscal Year: 2007
    ..The success of the training program is evidenced by the very strong publication record of the trainees and the very high quality of their subsequent postdoctoral positions. ..
  2. Preclinical Development of Flagellin as a Mucosal Adjuvant in Biodefense Vaccines
    STEVEN MIZEL; Fiscal Year: 2007
    ..The completion of these aims will provide a very strong foundation for the clinical evaluation of the mucosal flagellin/Y. pestis vaccine in humans. ..
  3. Activation of inflammatory cells by flagellin
    STEVEN MIZEL; Fiscal Year: 2006
    ..Furthermore, the proposed studies should also provide insights into the factors that regulate TLR signaling and allow for complex patterns of host cell response. ..