Daniel B Kim-Shapiro

Summary

Affiliation: Wake Forest University
Country: USA

Publications

  1. pmc Mechanisms of nitrite bioactivation
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, United States Translational Science Center, Wake Forest University, Winston Salem, NC 27109, United States Electronic address
    Nitric Oxide 38:58-68. 2014
  2. ncbi request reprint Time resolved absorption study of the reaction of hydroxyurea with sickle cell hemoglobin
    D B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109 7507, USA
    Biochim Biophys Acta 1380:64-74. 1998
  3. ncbi request reprint Hemoglobin-nitric oxide cooperativity: is NO the third respiratory ligand?
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109 7507, USA
    Free Radic Biol Med 36:402-12. 2004
  4. pmc Storage lesion: role of red blood cell breakdown
    Daniel B Kim-Shapiro
    Department of Physics and the Translational Science Center, Wake Forest University, Winston Salem, North Carolina 27109, USA
    Transfusion 51:844-51. 2011
  5. ncbi request reprint Unraveling the reactions of nitric oxide, nitrite, and hemoglobin in physiology and therapeutics
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Arterioscler Thromb Vasc Biol 26:697-705. 2006
  6. ncbi request reprint The reaction of deoxy-sickle cell hemoglobin with hydroxyurea
    D B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC, USA
    Biochim Biophys Acta 1428:381-7. 1999
  7. pmc The potential of Angeli's salt to decrease nitric oxide scavenging by plasma hemoglobin
    Xiaojun He
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Free Radic Biol Med 44:1420-32. 2008
  8. ncbi request reprint Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin
    Swati Basu
    Department of Physics, Wake Forest University, 1834 Wake Forest Road, Winston Salem, North Carolina 27109, USA
    Nat Chem Biol 3:785-94. 2007
  9. pmc Estrogen therapy, independent of timing, improves cardiac structure and function in oophorectomized mRen2.Lewis rats
    Jewell A Jessup
    Department of Physiology and Pharmacology, Wake Forest School of Medicine, Winston Salem, NC 27157 1009, USA
    Menopause 20:860-8. 2013
  10. pmc Lack of allosterically controlled intramolecular transfer of nitric oxide from the heme to cysteine in the beta subunit of hemoglobin
    Kris T Huang
    Department of Biomedical Engineering, Wake Forest University, Winston Salem, NC 27109, USA
    Blood 107:2602-4. 2006

Research Grants

  1. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2007
  2. Nitrite and Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2007
  3. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2006
  4. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2005
  5. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2004
  6. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2003
  7. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2002

Collaborators

Detail Information

Publications56

  1. pmc Mechanisms of nitrite bioactivation
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, United States Translational Science Center, Wake Forest University, Winston Salem, NC 27109, United States Electronic address
    Nitric Oxide 38:58-68. 2014
    ..In this work, we review different proposed mechanisms of nitrite bioactivation, focusing on analysis of kinetics and experimental evidence for the relevance of each mechanism under different conditions. ..
  2. ncbi request reprint Time resolved absorption study of the reaction of hydroxyurea with sickle cell hemoglobin
    D B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109 7507, USA
    Biochim Biophys Acta 1380:64-74. 1998
    ..It is proposed that methemoglobin and possibly nitric oxide-hemoglobin formation may partially explain beneficial effects observed in these patients before their levels of fetal hemoglobin have increased...
  3. ncbi request reprint Hemoglobin-nitric oxide cooperativity: is NO the third respiratory ligand?
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109 7507, USA
    Free Radic Biol Med 36:402-12. 2004
    ..Despite these factors the possibility that NO is delivered by hemoglobin under certain conditions or through more complicated mechanisms needs further study...
  4. pmc Storage lesion: role of red blood cell breakdown
    Daniel B Kim-Shapiro
    Department of Physics and the Translational Science Center, Wake Forest University, Winston Salem, North Carolina 27109, USA
    Transfusion 51:844-51. 2011
    ..Stored blood may also be deficient in recently discovered blood NO synthase activity. We hypothesize that these factors play a potential role in the blood storage lesion...
  5. ncbi request reprint Unraveling the reactions of nitric oxide, nitrite, and hemoglobin in physiology and therapeutics
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Arterioscler Thromb Vasc Biol 26:697-705. 2006
    ..As subsequently understood, the hypoxic generation of NO from nitrite is likely to prove important in many aspects of physiology, pathophysiology, and therapeutics...
  6. ncbi request reprint The reaction of deoxy-sickle cell hemoglobin with hydroxyurea
    D B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC, USA
    Biochim Biophys Acta 1428:381-7. 1999
    ....
  7. pmc The potential of Angeli's salt to decrease nitric oxide scavenging by plasma hemoglobin
    Xiaojun He
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Free Radic Biol Med 44:1420-32. 2008
    ..These results support the notion that Angeli's salt or a similar compound could be used to effectively treat conditions associated with intravascular hemolysis...
  8. ncbi request reprint Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin
    Swati Basu
    Department of Physics, Wake Forest University, 1834 Wake Forest Road, Winston Salem, North Carolina 27109, USA
    Nat Chem Biol 3:785-94. 2007
    ....
  9. pmc Estrogen therapy, independent of timing, improves cardiac structure and function in oophorectomized mRen2.Lewis rats
    Jewell A Jessup
    Department of Physiology and Pharmacology, Wake Forest School of Medicine, Winston Salem, NC 27157 1009, USA
    Menopause 20:860-8. 2013
    ....
  10. pmc Lack of allosterically controlled intramolecular transfer of nitric oxide from the heme to cysteine in the beta subunit of hemoglobin
    Kris T Huang
    Department of Biomedical Engineering, Wake Forest University, Winston Salem, NC 27109, USA
    Blood 107:2602-4. 2006
    ..We did not observe a decrease in the concentration of Fe-NO Hb on oxygenation, which is inconsistent with the prediction of the SNO-Hb hypothesis...
  11. ncbi request reprint Effects of iron nitrosylation on sickle cell hemoglobin solubility
    Xiuli Xu
    Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 277:36787-92. 2002
    ..Given the small amounts of NO-ligated hemoglobin achievable through any kind of NO therapy, we conclude that NO therapy does not benefit patients through any direct solubilizing effect...
  12. ncbi request reprint Hemoglobin mediated nitrite activation of soluble guanylyl cyclase
    Anne Jeffers
    Department of Physics, Wake Forest University, Winston Salem, NC, 27109 7507 USA
    Comp Biochem Physiol A Mol Integr Physiol 142:130-5. 2005
    ..Thus, an intermediate species must be formed by the nitrite/deoxyhemoglobin reaction that escapes the red cell and effects vasodilation...
  13. ncbi request reprint The reaction between nitrite and deoxyhemoglobin. Reassessment of reaction kinetics and stoichiometry
    Kris T Huang
    Department of Biomedical Engineering, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    J Biol Chem 280:31126-31. 2005
    ....
  14. ncbi request reprint Hydroxyurea analogues as kinetic and mechanistic probes of the nitric oxide producing reactions of hydroxyurea and oxyhemoglobin
    Jinming Huang
    Departments of Chemistry and Physics, Wake Forest University, Winston Salem, NC 27109, USA
    J Med Chem 46:3748-53. 2003
    ..These results should guide the development of new hydroxyurea-based nitric oxide donors and sickle cell disease therapies...
  15. ncbi request reprint Nitric oxide scavenging by red blood cells as a function of hematocrit and oxygenation
    Ivan Azarov
    Department of Physics, School of Medicine, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 280:39024-32. 2005
    ..These observations may have important physiological implications within the microvasculature and for pathophysiological disruption of nitric oxide homeostasis in diseases...
  16. ncbi request reprint Urease enhances the formation of iron nitrosyl hemoglobin in the presence of hydroxyurea
    Virginia L Lockamy
    Department of Physics, Wake Forest University, Winston Salem, NC 27109 7507, USA
    Biochim Biophys Acta 1622:109-16. 2003
    ..We show that addition of urease, which converts HU to HA, to a mixture of blood and HU, greatly enhances HbNO formation...
  17. pmc Nitrite reductase activity of cytochrome c
    Swati Basu
    Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 283:32590-7. 2008
    ..These data suggest an additional role for cytochrome c as a nitrite reductase that may play an important role in regulating mitochondrial function and contributing to hypoxic, redox, and apoptotic signaling within the cell...
  18. pmc A novel role for cytochrome c: Efficient catalysis of S-nitrosothiol formation
    Swati Basu
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Free Radic Biol Med 48:255-63. 2010
    ....
  19. ncbi request reprint Rates of nitric oxide dissociation from hemoglobin
    Fouad Azizi
    Department of Physics, Wake Forest University, Winston Salem, NC 27109 7507, USA
    Free Radic Biol Med 39:145-51. 2005
    ..2 +/- 1.5 x 10(-4) s(-1). We have also found that the fastest NO dissociation rate is on the order of 10(-3) s(-1) and that NO dissociation from sickle cell hemoglobin is the same as that from normal adult hemoglobin...
  20. ncbi request reprint Rat liver-mediated metabolism of hydroxyurea to nitric oxide
    Jinming Huang
    Department of Chemistry, Wake Forest University, Winston Salem, NC 27109, USA
    Free Radic Biol Med 40:1675-81. 2006
    ..These results reveal the ability of liver tissue to convert hydroxyurea to nitric oxide and provide insight into the metabolism of this drug...
  21. pmc Measurements of nitric oxide on the heme iron and beta-93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation
    Xiuli Xu
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Proc Natl Acad Sci U S A 100:11303-8. 2003
    ..Our results do not support a role for allosterically controlled intramolecular transfer of nitric oxide in hemoglobin as a function of oxygen saturation...
  22. pmc Mechanisms of slower nitric oxide uptake by red blood cells and other hemoglobin-containing vesicles
    Ivan Azarov
    Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 286:33567-79. 2011
    ..These results are consistent with prior studies indicating that extracellular diffusion of several gas ligands is also rate-limiting for erythrocytes, with some contribution of a low membrane permeability...
  23. pmc Neuronal nitric oxide synthase inhibition improves diastolic function and reduces oxidative stress in ovariectomized mRen2.Lewis rats
    Jewell A Jessup
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157 1009, USA
    Menopause 18:698-708. 2011
    ..Lewis rats treated with N5-(1-imino-3-butenyl)-L-ornithine (L-VNIO; 0.5 mg/kg per day for 28 d) or vehicle (saline)...
  24. pmc Computation of plasma hemoglobin nitric oxide scavenging in hemolytic anemias
    Anne Jeffers
    Department of Biomedical Engineering, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Free Radic Biol Med 41:1557-65. 2006
    ..These results support the paradigm that cell-free hemoglobin released into plasma during intravascular hemolysis in human disease contributes to the experimentally observed reduction in NO bioavailability and endothelial dysfunction...
  25. pmc Rate of nitric oxide scavenging by hemoglobin bound to haptoglobin
    Ivan Azarov
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Nitric Oxide 18:296-302. 2008
    ....
  26. ncbi request reprint Iron nitrosyl hemoglobin formation from the reaction of hydroxylamine and hemoglobin under physiological conditions
    Virginia L Lockamy
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Biochim Biophys Acta 1674:260-7. 2004
    ..These results indicate that the reaction of hydroxylamine with hemoglobin produces HbNO and lend chemical support for a potential role for hydroxylamine in the in vivo metabolism of hydroxyurea...
  27. pmc Nitroxyl accelerates the oxidation of oxyhemoglobin by nitrite
    Landon Bellavia
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Nitric Oxide 31:38-47. 2013
    ....
  28. pmc An electron paramagnetic resonance study of the affinity of nitrite for methemoglobin
    Bradley I Goetz
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Nitric Oxide 22:149-54. 2010
    ..These data suggest additional interesting chemistry in the reaction of nitrite with ferric and ferrous heme species. Moreover, this reaction could serve as a paradigm for ferric heme reactions with nitrite...
  29. ncbi request reprint Catalase-mediated nitric oxide formation from hydroxyurea
    Jinming Huang
    Departments of Chemistry and Physics, Wake Forest University, Winston Salem, NC 27109, USA
    J Med Chem 47:3495-501. 2004
    ..These results demonstrate nitric oxide production from the ferric catalase oxidation of nitroxyl and identify a catalase-mediated pathway as a potential source of nitric oxide production from hydroxyurea...
  30. pmc Effects of a single sickling event on the mechanical fragility of sickle cell trait erythrocytes
    TENNILLE D PRESLEY
    Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, USA
    Hemoglobin 34:24-36. 2010
    ..We find that the mechanical fragility of these cells increases dramatically after a single sickling event, suggesting that a substantial amount of hemolysis in vivo probably occurs in polymer-containing cells...
  31. doi request reprint Chemiluminescent detection of S-nitrosated proteins: comparison of tri-iodide, copper/CO/cysteine, and modified copper/cysteine methods
    Swati Basu
    Department of Physics, Wake Forest University, Winston Salem, North Carolina, USA
    Methods Enzymol 440:137-56. 2008
    ....
  32. pmc Kinetics of increased deformability of deoxygenated sickle cells upon oxygenation
    Zhi Huang
    Departments of Physics and Chemistry, Wake Forest University, Winston Salem, North Carolina 27109 USA
    Biophys J 85:2374-83. 2003
    ..These data imply that persistence of sickle cell hemoglobin polymers during circulation in vivo is likely, due to slow and incomplete polymer melting, contributing to the pathophysiology of sickle cell disease...
  33. ncbi request reprint Kinetics of nitric oxide binding to R-state hemoglobin
    Zhi Huang
    Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, USA
    Biochem Biophys Res Commun 292:812-8. 2002
    ..1 +/- 0.1) x 10(7) (s(-1) M(-1)), which is essentially the same as that reported for T-state NO binding. (c)2002 Elsevier Science (USA)...
  34. ncbi request reprint The reaction between nitrite and hemoglobin: the role of nitrite in hemoglobin-mediated hypoxic vasodilation
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC, USA
    J Inorg Biochem 99:237-46. 2005
    ....
  35. ncbi request reprint Iron nitrosyl hemoglobin formation from the reactions of hemoglobin and hydroxyurea
    Jinming Huang
    Department of Chemistry, Wake Forest University, Winston Salem, North Carolina 27109, USA
    Biochemistry 41:2466-74. 2002
    ..These direct nitric oxide producing reactions of hydroxyurea and hemoglobin may contribute to the overall pathophysiological properties of this drug...
  36. pmc Direct and nitroxyl (HNO)-mediated reactions of acyloxy nitroso compounds with the thiol-containing proteins glyceraldehyde 3-phosphate dehydrogenase and alkyl hydroperoxide reductase subunit C
    Susan Mitroka
    Department of Chemistry and Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, United States
    J Med Chem 56:6583-92. 2013
    ..These results suggest that nitroso compounds form a general class of thiol-modifying compounds, allowing their further exploration. ..
  37. ncbi request reprint Horseradish peroxidase catalyzed nitric oxide formation from hydroxyurea
    Jinming Huang
    Department of Chemistry, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Am Chem Soc 124:3473-80. 2002
    ..These studies suggest that direct nitric oxide producing reactions of hydroxyurea and peroxidases may contribute to the overall pharmacological properties of this drug...
  38. ncbi request reprint Hemoglobin effects in the Saville assay
    Swati Basu
    Department of Physics, Wake Forest University, Winston Salem, NC 27109 7507, USA
    Nitric Oxide 15:1-4. 2006
    ..Here we show that, on the contrary, the presence of sufficient hemoglobin in the Saville assay decreases its sensitivity...
  39. pmc Aggregation of normal and sickle hemoglobin in high concentration phosphate buffer
    Kejing Chen
    Department of Physics, Wake Forest University, Winston Salem, North Carolina, USA
    Biophys J 87:4113-21. 2004
    ..These studies contribute to the working knowledge of how to properly apply studies of hemoglobin S polymerization that are conducted using the high phosphate model...
  40. pmc Nitric oxide red blood cell membrane permeability at high and low oxygen tension
    Kris T Huang
    Virginia Tech Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Nitric Oxide 16:209-16. 2007
    ..The permeability changes by a factor of 10 or more upon oxygenation of anoxic RBCs. These results may have important implications with respect to NO import or export in physiology...
  41. pmc In vivo reduction of cell-free methemoglobin to oxyhemoglobin results in vasoconstriction in canines
    Dong Wang
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland Division of Pulmonary, Allergy and Critical Care Medicine and the Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania Department of Physics and the Translational Science Center, Wake Forest University, Winston Salem, North Carolina Anesthesia and Critical Care Medicine Department, West China Hospital of Sichuan University, Cheng Du, China Critical Care Medicine Department, Hospital Universitario de Getafe, Getafe, Madrid, Spain
    Transfusion 53:3149-63. 2013
    ..To clarify this, we tested the vascular effect and fate of oxyHb or metHb infusions...
  42. pmc Acute effect of a high nitrate diet on brain perfusion in older adults
    TENNILLE D PRESLEY
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Nitric Oxide 24:34-42. 2011
    ..These conditions could include age-associated dementia and cognitive decline. The goal of this study was to determine if dietary nitrate would increase cerebral blood flow in older adults...
  43. pmc Nitric oxide scavenging by red cell microparticles
    Chen Liu
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Free Radic Biol Med 65:1164-73. 2013
    ....
  44. doi request reprint Red blood cell endothelial nitric oxide synthase does not modulate red blood cell storage hemolysis
    Tamir Kanias
    Vascular Medicine Institute and the Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Transfusion 53:981-9. 2013
    ..This study examines the correlation between RBC eNOS deficiency and the propensity of RBCs to hemolyze under selected stress conditions including prolonged hypothermic storage...
  45. pmc Tetrahydrobiopterin restores diastolic function and attenuates superoxide production in ovariectomized mRen2.Lewis rats
    Jewell A Jessup
    Department of Anesthesiology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, North Carolina 27157 1009, USA
    Endocrinology 152:2428-36. 2011
    ..Whether BH₄ supplementation is a therapeutic option for the management of diastolic dysfunction in postmenopausal women will require direct testing in humans...
  46. ncbi request reprint Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:12916-27. 2007
    ....
  47. ncbi request reprint The emerging biology of the nitrite anion
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 1:308-14. 2005
    ..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics...
  48. ncbi request reprint Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation
    Mark T Gladwin
    Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1454, USA
    Am J Physiol Heart Circ Physiol 291:H2026-35. 2006
    ..An expanded consideration of nitrite as a hypoxia-dependent intrinsic signaling molecule has opened up a new field of research and therapeutic opportunities for diseases associated with regional hypoxia and vasoconstriction...
  49. ncbi request reprint Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
    Kenyatta Cosby
    Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 7B15 Bethesda, Maryland 20892, USA
    Nat Med 9:1498-505. 2003
    ..These results suggest that nitrite represents a major bioavailable pool of NO, and describe a new physiological function for hemoglobin as a nitrite reductase, potentially contributing to hypoxic vasodilation...
  50. pmc Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
    Zhi Huang
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, and Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 115:2099-107. 2005
    ..These data suggest that the oxygen sensor for hypoxic vasodilation is determined by Hb oxygen saturation and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under allosteric and pH control...
  51. pmc The functional nitrite reductase activity of the heme-globins
    Mark T Gladwin
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA
    Blood 112:2636-47. 2008
    ..We will review these reactions in the biologic framework of hypoxic signaling in blood and the heart...
  52. ncbi request reprint How do red blood cells dilate blood vessels?--Reply
    Daniel B Kim-Shapiro
    Circ Res 95:e10. 2004
  53. ncbi request reprint Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator
    Christian J Hunter
    Critical Care Medicine Department, Clinical Center, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892 1662, USA
    Nat Med 10:1122-7. 2004
    ....
  54. ncbi request reprint Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinity
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:3628-38. 2008
    ..From a pathophysiological standpoint, under conditions where HbS polymers form, the rate of nitrite reduction is reduced compared with HbA and solution-phase HbS, indicating that HbS polymers reduce nitrite more slowly...
  55. ncbi request reprint Detecting physiologic fluctuations in the S-nitrosohemoglobin micropopulation: Triiodide versus 3C
    Allan Doctor
    Blood 108:3225-6; author reply 3226-7. 2006

Research Grants10

  1. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2007
    ..Techniques have been developed so that studies can be made on whole blood, so that physiologically relevant conditions can be assessed. ..
  2. Nitrite and Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2007
    ..The candidate currently has a heavy teaching and service load. A K02 award will relieve him of some of these duties so that he can spend more time interacting with his colleagues, attending seminars and meetings, and doing research. ..
  3. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2006
    ..Our studies will help us understand important issues related to NO's role in sickle cell disease and in the general population. ..
  4. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2005
    ..Our studies will help us understand important issues related to NO's role in sickle cell disease and in the general population. ..
  5. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2004
    ..Our studies will help us understand important issues related to NO's role in sickle cell disease and in the general population. ..
  6. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2003
    ..Our studies will help us understand important issues related to NO's role in sickle cell disease and in the general population. ..
  7. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2002
    ..Our studies will help us understand important issues related to NO's role in sickle cell disease and in the general population. ..