A Atala

..If a tubularized urethral repair is needed, the use of cells on the collagen matrix is essential for adequate tissue formation. Tissue engineering techniques are useful for urethral reconstruction...

..This article discusses these tissue engineering strategies for various organs in the male and female reproductive tract...

..While stem cells are still in the research phase, some therapies arising from tissue engineering endeavors have already entered the clinical setting successfully, indicating the promise regenerative medicine holds for the future...

..Blast cells are multipotent and can differentiate into multiple lineages of hematopoeitic cells (erythroid, granulocyte and macrophage), endothelial and smooth muscle cells...

..This chapter reviews recent advances that have occurred in the regeneration of urologic organs and describes how these applications may offer novel therapies for patients with urologic disease...

..The principles of tissue engineering will be discussed as well as some current strategies for applying these principles in developing novel therapies for urinary incontinence...

..This goal may soon be attainable with the use of tissue engineering techniques...

..This paper reviews recent advances that have occurred in regenerative medicine and describes applications of these new technologies that may offer novel therapies for patients with end-stage organ failure...

..The present chapter reviews recent advances that have occurred in therapeutic cloning and tissue engineering and describes applications of these new technologies that may offer novel therapies for patients with end-stage organ failure...

..This paper reviews recent advances that have occurred in regenerative medicine and describes application of new technologies to treat diseased or damaged organs and tissues...

..This paper reviews recent advances in stem cell research and regenerative medicine, and describes the clinical applications of these technologies as novel therapies for tissue or organ loss...

..This review covers recent advances that have occurred in regenerative medicine and describes applications of these technologies using chemical compounds that may offer novel therapies for patients with end-stage organ failure...

..This article reviews some of the principles of tissue engineering and some of the applications of these principles to the genitourinary tract...

..This article reviews recent advances in regenerative medicine and describes applications of biological substitutes that may offer novel therapies for patients with end-stage organ failure...

..This paper reviews recent advances that have occurred in regenerative medicine and describes applications of new technologies to treat diseased or damaged organs and tissues...

..The presence of such segments in the urinary tract has been associated with many complications. We explored an alternative approach using autologous engineered bladder tissues for reconstruction...

..Recent progress suggests that engineered urologic tissues and cell therapy may have clinical applicability...

..There is a growing optimism that the successes in this field will achieve the goal of significantly extending the life of the patient...

..Although stem cells are still in the research phase, some therapies arising from tissue engineering endeavors have already entered the clinical setting successfully, indicating the promise regenerative medicine holds for the future...

..Further research to develop novel biomaterials and cell sources, as well as information gained from developmental biology, signal transduction studies and studies of the wound healing response would be beneficial...

..While stem cells are still in the research phase, some therapies arising from tissue engineering endeavors have already entered the clinical setting, indicating that regenerative medicine holds promise for the future...

..Recent progress suggests that engineered genitourinary tissues may have clinical applicability in the future...

..We show that the bilayered scaffolds permit EC adhesion on the lumen and SMC infiltration into the outer layer. This study suggests that the use of bilayered scaffolds may lead to improved vessel formation...

..As the science of tissue engineering evolves, one can expect a wider application of this technology to the armamentarium of urologic surgery...

..The present chapter reviews recent advances that have occurred in therapeutic cloning and tissue engineering and describes applications of these new technologies that may offer novel therapies for patients with end-stage organ failure...

..Our results demonstrate that myogenic-differentiated BMSC seeded on a nano fibrous PLLA scaffold can be potentially used for cell-based tissue engineering for bladder cancer patients requiring cystoplasty...

..Recent progress suggests that engineered urologic tissues may have an expanded clinical applicability in the future...

..BJU Int 2000; 85: 715; Lai and Chang, Changgeng Yi Xue Za Zhi 1999; 22: 253; Parsons et al., J Pediatr Surg 2002; 37: 629; Seccia et al., Ann Plast Surg 2002; 49: 379; Filipas, Curr Opin Urol 2001; 11: 267)...

..The collagen matrices seeded with cells may offer a useful alternative in the future for patients requiring a tubularized urethral segment replacement...

..This study demonstrates that dynamic cell attachment, using a bioreactor system, leads to the formation of morphologically and biochemically improved corporal tissue, which may be useful for penile reconstruction...

..This study provides evidence that cultured human corporal smooth muscle cells may be used in conjunction with biodegradable polymer scaffolds to create corpus cavernosum smooth muscle tissue in vitro and in vivo...

..Patients were randomized in a double-blinded manner to receive either ketorolac or placebo after the surgery. We demonstrate that ketorolac reduces the frequency and severity of postoperative bladder spasm...

..We also discuss the use of amniotic-fluid derived stem cells (AFS) for potential patient-specific therapies...

..Trials involving bladder replacement using tissue engineering techniques currently are being arranged. Recent progress suggests that engineered urologic tissues may have clinical applicability...

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..This indicates that improved tissue viability could be maintained for several days using oxygen producing biomaterials...

..Clinical translation of these new classes of biomaterials will be supported by many of the same evaluation tools as those developed and described by Professor David F. Williams and colleagues over the past 30 years...

..This study suggests that the introduction of thermal fiber bonding to electrospun PCL scaffolds improved the biomechanical properties of these scaffolds, making them more suitable for tissue engineering applications...

..Therefore, research efforts in this field have recently focused on improving our ability to diagnose clinically relevant obstructive uropathy and examining the developmental causes of megaureter, and how this disorder may be prevented...

..This review describes recent advances in the engineering of several key tissues and organs...

..The aligned composite nanofiber scaffolds seeded with skeletal muscle cells may provide implantable functional muscle tissues for patients with large muscle defects...

..Scaffolds with such characteristics have potential applications in cell-based urological tissue engineering...

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..To address this problem, we have developed an extracorporeal cellular affinity (ECA) column that can recover CD133 expressing progenitor cells with high efficiency...

..Such scaffolds may play an important role in tissue engineering where currently oxygen diffusion limits the engineering of large tissue implants...

..This study suggests that electrospun scaffolds combined with vascular cells may become an alternative to prosthetic vascular grafts for vascular reconstruction...

..Physicians should be optimistic that regenerative medicine and tissue engineering will one day provide mainstream treatment options for urologic disorders...

..We determined whether the cells present in urine have characteristics of normal bladder cells and investigated their potential uses for urological reconstructive procedures...

..Therefore, it may be possible to enrich the infiltrate with such cell types and control their fate, provided the proper substrate-mediated signaling can be imparted into the scaffold for in situ tissue regeneration...

..This scaffold system may be useful in patients requiring hollow organ replacement...

..This study demonstrates that neocorpora can be engineered for total pendular penile corporal body replacement. This technology has considerable potential for patients requiring penile reconstruction...

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..This study shows that acellular biomaterials derived from human dermis are suitable for abdominal hernia repair...

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..The present chapter reviews recent advances that have occurred in therapeutic cloning and tissue engineering and describes applications of these new technologies that may offer novel therapies for patients with end-stage organ failure...

..This study demonstrates the promise of electrospinning as a fabrication process for a functional vascular graft for clinical use...

..Use of the composite scaffolding system with cells may enhance the formation of bone tissue for therapeutic regeneration...

..Overall, carbon nanotubes may play an integral role as unique biomaterial for creating and monitoring engineered tissue...

..Tissue-engineered vessels may potentially be useful clinically as vascular grafts...

..This study demonstrates the feasibility of creating human cartilage rods with a large dimension. This technology may be useful for patients who need penile reconstruction...

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..Recent progress suggests that engineered urologic tissues may have clinical applicability in the future...

..This technology may be useful clinically for stricture disease in the genitourinary tract...

..Recent progress suggests that engineered urologic tissues may have clinical applicability in the future...

..In conclusion, smooth muscle- and urothelium-like cells derived from human BMSCs provide an alternative cell source for potential use in bladder tissue engineering...

..These bioscaffolds can further provide a tool to study cells in their natural three-dimensional environment, which is superior for drug discovery platform compared with cells cultured in two-dimensional petri dishes...

..CONCLUSIONS: Use of an off-the-shelf collagen inert matrix appears to be beneficial for patients with urethral strictures and obviates the need for obtaining an autologous graft, thus eliminating donor site morbidity...

..In the setting of sphincter insufficiency, injection of MPCs at different stages of maturation may be a better option than purified muscle stem cells...

..Banking strategies to identify small numbers of stem cell lines homozygous for major histocompatibility loci have been proposed to enable beneficial genetic matching that would decrease the need for immunosuppression...

..We investigated the maximum potential distance of normal native tissue regeneration over a range of distances using acellular matrices for tubular grafts as an experimental model...

..This injectable hybrid system composed of degradable and non-degradable hydrogels may be a preferable approach to the repair of soft tissue defects...

..We report the incidence of VUR in siblings of multiple gestation births and for the first time compare the relative incidence of reflux between identical and fraternal twins...

..The technique has few complications as well as proved success and versatility that continues to expand its applicability and popularity...

..This paper reviews recently described techniques used to decellularize whole organs such as the heart, lung, liver and kidney and describes possible methods for using these matrices for whole organ engineering...

..Further, we will highlight different opportunities for trainees to obtain significant research exposure in urology...

..Similar studies are also being conducted in our laboratory on encapsulation of ovarian cells for the secretion of progesterone and estrogen in culture and in vivo using microencapsulation techniques...

..This article reviews the process of blood-vessel growth and tissue vascularization, placing emphasis on strategies that can be employed for efficient vascularization of engineered tissues in vitro and in vivo...

..These results show that ABM is biocompatible, durable and effective when used as a tunica substitute. The matrix may be useful as an off-the-shelf biomaterial for patients requiring tunica albuginea repair...

..Our studies indicate that VEGF and VEGF receptor expression in NBL tumor cells are associated with tumor growth and that angiogenic factors may serve as a biological marker together with already established MYCN amplification...

..Emphasis is placed upon investigations that are more directly linked to the treatment of clinical conditions...

..The ability to generate histocompatible cells using cloning techniques addresses one of the major challenges in transplantation medicine...

..This paper reviews the process of blood vessel growth and tissue vascularization, and discuss strategies for efficient vascularization of engineered tissues...

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..This finding has important implications to the application of tissue engineering and regenerative medicine to skeletal muscle replacement and reconstruction...

..The PCL/collagen composite scaffolds are biocompatible, possess biomechanical properties that resist high degrees of pressurized flow over long term, and provide a favorable environment that supports the growth of vascular cells...

..These results report for the first time a novel property of gold nanoparticles to bind heparin-binding proteins and thereby inhibit their subsequent signaling events...

..Understanding the relevant molecular pathways of stem cell repair phenotype should be a priority, in our view, for the entire stem cell field...

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..Recent studies indicate that genetic ablation of mouse uroplakin (UP) III gene, which encodes a 47 kD urothelial-specific integral membrane protein forming urothelial plaques, causes VUR and hydronephrosis...

..33 x 10(5) cells/ml). This system is useful for the seeding of biomaterials having cylindrical or planar geometries, and may be used under conditions that require low cell numbers and/or short seeding time periods...

..This study demonstrates that human cavernosal smooth muscle and endothelial cells seeded on three-dimensional acellular collagen matrices derived from donor corpora are able to form well-vascularized corporal tissues in vivo...

..The authors investigated the fate of muscle precursor cells (MPC) injected into a model of striated urethral sphincter injury that reproduces the histopathologic changes of sphincter insufficiency...

..This technology may be pursued further experimentally in order to achieve the engineering of vaginal tissues for clinical applications...

..Successful development of an abundant source of transplantable insulin producing cells potentially would have a profound impact on the treatment of a major public health problem. ..

..In this aim we will attempt to determine the phenotypic and functional characteristics, over time, of the bioengineered bladder tissues, as compared to controls. ..

..Successful development of an abundant source of transplantable insulin producing cells potentially would have a profound impact on the treatment of a major public health problem. ..