Alexey L Khodjakov

Summary

Affiliation: Wadsworth Center
Country: USA

Publications

  1. pmc The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules
    A Khodjakov
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201 0509, USA
    J Cell Biol 146:585-96. 1999
  2. pmc The nature of cell-cycle checkpoints: facts and fallacies
    Alexey Khodjakov
    Wadsworth Center, Albany, NY 12201 0509, USA
    J Biol 8:88. 2009
  3. ncbi request reprint Laser microsurgery in fission yeast; role of the mitotic spindle midzone in anaphase B
    Alexey Khodjakov
    New York State Department of Health, Wadsworth Center, Albany 12201, USA
    Curr Biol 14:1330-40. 2004
  4. pmc Mitosis: too much of a good thing (can be bad)
    Alexey Khodjakov
    Wadsworth Center, Albany, NY 12201 0509, USA
    Curr Biol 19:R1032-4. 2009
  5. pmc De novo formation of centrosomes in vertebrate cells arrested during S phase
    Alexey Khodjakov
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 158:1171-81. 2002
  6. pmc Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression
    A Khodjakov
    Laboratory of Cell Regulation, Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201, USA
    J Cell Biol 153:237-42. 2001
  7. ncbi request reprint Centrosome-independent mitotic spindle formation in vertebrates
    A Khodjakov
    Division of Molecular Medicine, New York State Department of Health, Department of Biomedical Sciences, Wadsworth Center, State University of New York, Albany, 12201 0509, 12222, USA
    Curr Biol 10:59-67. 2000
  8. pmc Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis
    Alexey Khodjakov
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 160:671-83. 2003
  9. ncbi request reprint Multiple mechanisms regulate NuMA dynamics at spindle poles
    Olga Kisurina-Evgenieva
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Sci 117:6391-400. 2004
  10. pmc Drosophila CLASP is required for the incorporation of microtubule subunits into fluxing kinetochore fibres
    Helder Maiato
    Laboratory of Cell Regulation, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201 0509, USA
    Nat Cell Biol 7:42-7. 2005

Research Grants

  1. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey L Khodjakov; Fiscal Year: 2010
  2. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2009
  3. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2005
  4. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2001
  5. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2009

Collaborators

Detail Information

Publications31

  1. pmc The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules
    A Khodjakov
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201 0509, USA
    J Cell Biol 146:585-96. 1999
    ..Rather, at the onset of mitosis, the centrosome suddenly gains the ability to bind greater than three times the amount of gamma-tubulin than during interphase...
  2. pmc The nature of cell-cycle checkpoints: facts and fallacies
    Alexey Khodjakov
    Wadsworth Center, Albany, NY 12201 0509, USA
    J Biol 8:88. 2009
    ..We illustrate, using the mitotic checkpoint, problems that can arise from a failure to respect strict definitions and precise terminology...
  3. ncbi request reprint Laser microsurgery in fission yeast; role of the mitotic spindle midzone in anaphase B
    Alexey Khodjakov
    New York State Department of Health, Wadsworth Center, Albany 12201, USA
    Curr Biol 14:1330-40. 2004
    ..To probe the mechanism of spindle elongation, we combine fluorescence microscopy, photobleaching, and laser microsurgery in the fission yeast Schizosaccharomyces pombe...
  4. pmc Mitosis: too much of a good thing (can be bad)
    Alexey Khodjakov
    Wadsworth Center, Albany, NY 12201 0509, USA
    Curr Biol 19:R1032-4. 2009
    ..Kinetochore microtubule attachments that are too stable increase the rate of chromosome mis-segregation, a leading cause of chromosomal instability in tumors...
  5. pmc De novo formation of centrosomes in vertebrate cells arrested during S phase
    Alexey Khodjakov
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 158:1171-81. 2002
    ..Although clouds of PCM consistently form even when microtubules are completely disassembled by nocodazole, the centrioles are not assembled under these conditions...
  6. pmc Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression
    A Khodjakov
    Laboratory of Cell Regulation, Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201, USA
    J Cell Biol 153:237-42. 2001
    ..These data reveal that the primary role of the centrosome in somatic cells is not to form the spindle but instead to ensure cytokinesis and subsequent cell cycle progression...
  7. ncbi request reprint Centrosome-independent mitotic spindle formation in vertebrates
    A Khodjakov
    Division of Molecular Medicine, New York State Department of Health, Department of Biomedical Sciences, Wadsworth Center, State University of New York, Albany, 12201 0509, 12222, USA
    Curr Biol 10:59-67. 2000
    ..By a combination of labeling with green fluorescent protein (GFP) and laser microsurgery we have been able to selectively destroy centrosomes in living mammalian cells as they enter mitosis...
  8. pmc Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis
    Alexey Khodjakov
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 160:671-83. 2003
    ..We propose that the capture and incorporation of preformed K-fibers complements the microtubule plus-end capture mechanism and contributes to spindle formation in vertebrates...
  9. ncbi request reprint Multiple mechanisms regulate NuMA dynamics at spindle poles
    Olga Kisurina-Evgenieva
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Sci 117:6391-400. 2004
    ....
  10. pmc Drosophila CLASP is required for the incorporation of microtubule subunits into fluxing kinetochore fibres
    Helder Maiato
    Laboratory of Cell Regulation, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201 0509, USA
    Nat Cell Biol 7:42-7. 2005
    ....
  11. pmc Extra centrosomes and/or chromosomes prolong mitosis in human cells
    Zhenye Yang
    Division of Molecular Medicine, Wadsworth Center, N Y S Department of Health, Empire State Plaza, Albany, New York 12201 0509, USA
    Nat Cell Biol 10:748-51. 2008
    ..Thus mitosis can be prolonged by non-genetic means and extra chromosomes and centrosomes probably contribute to the elevated mitotic index seen in many tumours...
  12. pmc Pushing forces drive the comet-like motility of microtubule arrays in Dictyostelium
    Daniela A Brito
    Division of Molecular Medicine, Wadsworth Center, Albany, NY 12201, USA
    Mol Biol Cell 16:3334-40. 2005
    ....
  13. pmc Control of daughter centriole formation by the pericentriolar material
    Jadranka Loncarek
    Division of Molecular Medicine, Wadsworth Center, Albany, New York State Department of Health, Albany, New York 12201 0509, USA
    Nat Cell Biol 10:322-8. 2008
    ..We propose that that the size of the PCM cloud associated with the mother centriole restricts the number of daughters that can form simultaneously...
  14. ncbi request reprint Regulation of cytokinesis by spindle-pole bodies
    Valentin Magidson
    Wadsworth Center, Albany, NY 12201 0509, USA
    Nat Cell Biol 8:891-3. 2006
    ..Ablation of only the daughter SPB often leads to activation of the SIN on the mother SPB and successful cytokinesis. Thus, either SPB can drive cytokinesis...
  15. pmc Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis
    Helder Maiato
    Wadsworth Center, New York State Department of Health, Albany 12201, USA
    J Cell Biol 167:831-40. 2004
    ..However, centrosomes facilitate the proper orientation of K-fibers toward spindle poles by integrating them into a common spindle...
  16. pmc The centromere geometry essential for keeping mitosis error free is controlled by spindle forces
    Jadranka Loncarek
    Division of Molecular Medicine, Wadsworth Center, Albany, New York State Department of Health, Albany, New York 12201 0509, USA
    Nature 450:745-9. 2007
    ..Thus, proper architecture of the centromere makes an important contribution to achieving high fidelity of chromosome segregation...
  17. ncbi request reprint Cooperative mechanisms of mitotic spindle formation
    CHRISTOPHER B O'CONNELL
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201 0509, USA
    J Cell Sci 120:1717-22. 2007
    ..These data support a modified search-and-capture model that incorporates additional assembly pathways coordinated by a RanGTP gradient...
  18. pmc Ab ovo or de novo? Mechanisms of centriole duplication
    Jadranka Loncarek
    Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Albany, New York, 12201 0509, USA
    Mol Cells 27:135-42. 2009
    ..Here we review key proteins involved in centriole assembly, compare two major modes of centriole biogenesis, and discuss the mechanisms that ensure stringency of centriole number...
  19. pmc The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
    CHRISTOPHER B O'CONNELL
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 183:29-36. 2008
    ..Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension...
  20. pmc Relative contributions of chromatin and kinetochores to mitotic spindle assembly
    CHRISTOPHER B O'CONNELL
    Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 187:43-51. 2009
    ..These results support a model in which kinetochores play the dominant role in the chromosome-mediated pathway of mitotic spindle assembly...
  21. pmc Androgen and Src signaling regulate centrosome activity
    Diane Colello
    Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY 12208, USA
    J Cell Sci 123:2094-102. 2010
    ..Thus, we propose that androgen and Src signaling regulate microtubule nucleation during interphase by promoting the centrosomal localization of gamma-tubulin...
  22. pmc Laser microsurgery in the GFP era: a cell biologist's perspective
    Valentin Magidson
    Division of Molecular Medicine, Wadsworth Center, Albany, New York 12201, USA
    Methods Cell Biol 82:239-66. 2007
    ..Our major thesis is that the limitations of the technology are no longer at the level of the laser, microscope, or software, but instead only in defining creative questions and in visualizing the target to be destroyed...
  23. ncbi request reprint Mitosis through the microscope: advances in seeing inside live dividing cells
    Conly L Rieder
    Lab of Cell Regulation, Division of Molecular Medicine, Wadsworth Center, Albany, NY 12201 0509, USA
    Science 300:91-6. 2003
    ..Several new structures are then assembled to separate the chromosomes and partition the cytoplasm into two separate cells...
  24. ncbi request reprint Dynamic microtubules in Dictyostelium
    Michael P Koonce
    Division of Molecular Medicine, Wadsworth Center, Empire State of Plaza, P O Box 509, Albany, NY 12201 0509, USA
    J Muscle Res Cell Motil 23:613-9. 2002
    ..We propose that these forces are important both in maintaining the overall structure of the microtubule array and in supporting intracellular traffic...
  25. pmc Centriole reduplication during prolonged interphase requires procentriole maturation governed by Plk1
    Jadranka Loncarek
    Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201 0509, USA
    Curr Biol 20:1277-82. 2010
    ..Activation of Plk1 during late S/G2 induces procentriole maturation, and after this point, the centriole cycle can be completed autonomously, even in the absence of cell-cycle progression...
  26. ncbi request reprint Microtubule flux: what is it good for?
    Alexey Khodjakov
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201 0509, USA
    Curr Biol 15:R966-8. 2005
    ..A new study has revealed that inhibiting this microtubule flux in mammalian cells has no major effects on chromosome movements; it does, however, increase the probability of erroneous chromosome segregation...
  27. pmc The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions
    Yimin Dong
    Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, NY 12201, USA
    Nat Cell Biol 9:516-22. 2007
    ....
  28. pmc The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
    Sabrina La Terra
    Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Cell Biol 168:713-22. 2005
    ..By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway...
  29. pmc Imaging the division process in living tissue culture cells
    Alexey Khodjakov
    Wadsworth Center, New York State Department of Health, Albany, NY 12201 0509, USA
    Methods 38:2-16. 2006
    ..We discuss practical considerations for minimizing this damage, and for maintaining the general health of the cell, while it is being followed by multi-mode or multi-dimensional light microscopy...
  30. ncbi request reprint Increased lysosomal uptake of methotrexate-polyglutamates in two methotrexate-resistant cell lines with distinct mechanisms of resistance
    Lisa A Marshall
    Wadsworth Center, New York State Department of Health, Wadsworth Center, Empire State Plaza, Albany, NY 12201, USA
    Biochem Pharmacol 71:203-13. 2005
    ....
  31. ncbi request reprint The tetrameric molecule of conventional kinesin contains identical light chains
    Fatima K Gyoeva
    Institute of Protein Research, Russian Academy of Sciences, Moscow 117334, Russian Federation
    Biochemistry 43:13525-31. 2004
    ..These data suggest that incorporation of the two identical light chains into a single kinesin molecule most likely occurs cotranslationally...

Research Grants12

  1. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey L Khodjakov; Fiscal Year: 2010
    ....
  2. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2009
    ..Since centrosome abnormalities are a hallmark of malignant tumors, the knowledge obtained in these studies will be useful for designing new strategies for the treatment of cancers. ..
  3. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2005
    ..Since centrosome abnormalities are a hallmark of cell transformation and cancer, this knowledge will be useful for designing new strategies for the treatment of this disease. ..
  4. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2001
    ..g., as cancer, Alzheimer's, arthritis, etc.) ..
  5. FUNCTIONAL PROPERTIES OF CENTROSOMES IN SOMATIC CELLS
    Alexey Khodjakov; Fiscal Year: 2009
    ..Since centrosome abnormalities are a hallmark of malignant tumors, the knowledge obtained in these studies will be useful for designing new strategies for the treatment of cancers. ..