Research Topics
| Y H P ZhangSummaryAffiliation: Virginia Polytechnic Institute and State University Country: USA Publications
| Collaborators
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Detail Information
Publications
A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structureY-H Percival Zhang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, 24060, USA
Biomacromolecules 7:644-8. 2006- Outlook for cellulase improvement: screening and selection strategiesY H Percival Zhang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Biotechnol Adv 24:452-81. 2006..Herein, we hypothesize that continuous culture using insoluble cellulosic substrates could be a powerful selection tool for enriching beneficial cellulase mutants from the large library displayed on the cell surface... - Fractionating recalcitrant lignocellulose at modest reaction conditionsYi Heng Percival Zhang
Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
Biotechnol Bioeng 97:214-23. 2007..Isolation of high-value lignocellulose components (lignin, acetic acid, and hemicellulose) would greatly increase potential revenues of a lignocellulose biorefinery... 
Constructing the electricity-carbohydrate-hydrogen cycle for a sustainability revolutionY H Percival Zhang
Biological Systems Engineering Department, Virginia Tech, 304 Seitz Hall, Blacksburg, Virginia 24061, USA
Trends Biotechnol 30:301-6. 2012....- Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylaseXinhao Ye
Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
Mol Biosyst 8:1815-23. 2012..3 to 24.6 min. The temperature optimum of CM3 was increased from 60 to 80 °C. These results suggested that a combination of rational design and random mutagenesis could have a solid basis for engineering large proteins... 
Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilisChun You
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
Appl Environ Microbiol 78:1593-5. 2012..The DNA multimer generated by prolonged overlap extension PCR was directly transformed in Escherichia coli [e.g., TOP10, DH5α, JM109, and BL21(DE3)] and Bacillus subtilis for obtaining chimeric plasmids...- Enhanced microbial utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complexChun You
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA
Appl Environ Microbiol 78:1437-44. 2012..3- to 4.5-fold would help in the development of better consolidated bioprocessing microorganisms (e.g., B. subtilis) that can hydrolyze recalcitrant cellulose rapidly at low secretory cellulase levels... - Cell-free protein synthesis energized by slowly-metabolized maltodextrinYiran Wang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
BMC Biotechnol 9:58. 2009..Cell-free protein synthesis (CFPS) is a rapid and high throughput technology for obtaining proteins from their genes. The primary energy source ATP is regenerated from the secondary energy source through substrate phosphorylation in CFPS... - Substrate channeling and enzyme complexes for biotechnological applicationsY H Percival Zhang
Biological Systems Engineering Department, 210 A Seitz Hall, Virginia Tech, Blacksburg, VA 24061, USA
Biotechnol Adv 29:715-25. 2011.... - Biofuel production by in vitro synthetic enzymatic pathway biotransformationY H Percival Zhang
Biological Systems Engineering Department, 210 A Seitz Hall, Blacksburg, VA 24061, USA
Curr Opin Biotechnol 21:663-9. 2010..It is expected that high-yield SyPaB will be an important platform for producing low-cost biofuels and biochemicals... - Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regenerationYiran Wang
Biological Systems Engineering Department, 210 A Seitz Hall, Virginia Polytechnic Institute and State University, Blacksburg, Virgina 24061, USA
Microb Cell Fact 8:30. 2009..abstract:.. - A functionally based model for hydrolysis of cellulose by fungal cellulaseY H Percival Zhang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Biotechnol Bioeng 94:888-98. 2006..Opportunities to further refine cellulase kinetic models are discussed, including parameters that would benefit from further study... - Biosynthesis of radiolabeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugarsY H P Zhang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall 0303, Blacksburg, VA 24060, USA
Appl Microbiol Biotechnol 70:123-9. 2006..Results showed that the largest amount of radioactivity was cellobiose with lesser amounts of glucose, cellotriose, and cellotetraose, and an average DP of intracellular cellodextrins was ca. 2... - Reviving the carbohydrate economy via multi-product lignocellulose biorefineriesY H Percival Zhang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
J Ind Microbiol Biotechnol 35:367-75. 2008..g., 30%) of transportation fuels through liquid biofuels, internal combustion engines in the short term, and would provide 100% transportation fuels by sugar-hydrogen-fuel cell systems in the long term... - High-yield hydrogen production from starch and water by a synthetic enzymatic pathwayY H Percival Zhang
Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia, United States of America
PLoS ONE 2:e456. 2007.... - Methodological analysis for determination of enzymatic digestibility of cellulosic materialsY H Percival Zhang
Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Biotechnol Bioeng 96:188-94. 2007..50%)... - Efficient sugar release by the cellulose solvent-based lignocellulose fractionation technology and enzymatic cellulose hydrolysisGeoffrey Moxley
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall, Blacksburg, Virginia 24061, USA
J Agric Food Chem 56:7885-90. 2008..It was found that phosphoric acid only above a critical concentration (83%) with a sufficient reaction time can efficiently disrupt recalcitrant lignocellulose structures... - Quantitative determination of cellulose accessibility to cellulase based on adsorption of a nonhydrolytic fusion protein containing CBM and GFP with its applicationsJiong Hong
Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061, USA
Langmuir 23:12535-40. 2007..Declining heterogeneous cellulose reactivity is significantly attributed to a loss of CAC where the easily hydrolyzed cellulose fraction is digested first... - Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: heterologous expression, characterization, and synergy with family 48 cellobiohydrolaseXiao zhou Zhang
Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Bioresour Technol 101:5534-8. 2010..This study provided useful information for understanding of the cellulose hydrolysis mechanism of this cellulolytic bacterium with potential industrial importance... - A highly active phosphoglucomutase from Clostridium thermocellum: cloning, purification, characterization and enhanced thermostabilityY Wang
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
J Appl Microbiol 108:39-46. 2010..Discovery and utilization of highly active and thermostable phosphoglucomutase (PGM) would be vital for biocatalysis mediated by multiple enzymes, for example, high-yield production of enzymatic hydrogen... - Bioseparation of recombinant cellulose-binding module-proteins by affinity adsorption on an ultra-high-capacity cellulosic adsorbentJiong Hong
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall, Blacksburg, VA 24061, USA
Anal Chim Acta 621:193-9. 2008..Five recombinant proteins (CBM-protein), regardless of intercellular or periplasmic form, were purified successfully for demonstration purpose... - Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavageJiong Hong
Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall, Blacksburg, VA 24061, USA
J Chromatogr A 1194:150-4. 2008..0 to 6.5. Four recombinant proteins (green fluorescence protein, phosphoglucomutase, cellobiose phosphorylase, and glucan phosphorylase) have been purified successfully... - Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysisY-H Percival Zhang
Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
Biomacromolecules 6:1510-5. 2005..By contrast, the DP of Avicel remained nearly constant during hydrolysis. The specific enzymatic cellulose hydrolysis rate is 100-fold higher for PASC as compared to Avicel...