Y H P Zhang

Summary

Affiliation: Virginia Polytechnic Institute and State University
Country: USA

Publications

  1. ncbi request reprint A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, 24060, USA
    Biomacromolecules 7:644-8. 2006
  2. ncbi request reprint Outlook for cellulase improvement: screening and selection strategies
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Biotechnol Adv 24:452-81. 2006
  3. ncbi request reprint Fractionating recalcitrant lignocellulose at modest reaction conditions
    Yi Heng Percival Zhang
    Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
    Biotechnol Bioeng 97:214-23. 2007
  4. doi request reprint Constructing the electricity-carbohydrate-hydrogen cycle for a sustainability revolution
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Tech, 304 Seitz Hall, Blacksburg, Virginia 24061, USA
    Trends Biotechnol 30:301-6. 2012
  5. doi request reprint Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase
    Xinhao Ye
    Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
    Mol Biosyst 8:1815-23. 2012
  6. pmc Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis
    Chun You
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
    Appl Environ Microbiol 78:1593-5. 2012
  7. pmc Enhanced microbial utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex
    Chun You
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA
    Appl Environ Microbiol 78:1437-44. 2012
  8. pmc Cell-free protein synthesis energized by slowly-metabolized maltodextrin
    Yiran Wang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
    BMC Biotechnol 9:58. 2009
  9. doi request reprint Substrate channeling and enzyme complexes for biotechnological applications
    Y H Percival Zhang
    Biological Systems Engineering Department, 210 A Seitz Hall, Virginia Tech, Blacksburg, VA 24061, USA
    Biotechnol Adv 29:715-25. 2011
  10. doi request reprint Biofuel production by in vitro synthetic enzymatic pathway biotransformation
    Y H Percival Zhang
    Biological Systems Engineering Department, 210 A Seitz Hall, Blacksburg, VA 24061, USA
    Curr Opin Biotechnol 21:663-9. 2010

Collaborators

Detail Information

Publications23

  1. ncbi request reprint A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, 24060, USA
    Biomacromolecules 7:644-8. 2006
  2. ncbi request reprint Outlook for cellulase improvement: screening and selection strategies
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Biotechnol Adv 24:452-81. 2006
    ..Herein, we hypothesize that continuous culture using insoluble cellulosic substrates could be a powerful selection tool for enriching beneficial cellulase mutants from the large library displayed on the cell surface...
  3. ncbi request reprint Fractionating recalcitrant lignocellulose at modest reaction conditions
    Yi Heng Percival Zhang
    Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
    Biotechnol Bioeng 97:214-23. 2007
    ..Isolation of high-value lignocellulose components (lignin, acetic acid, and hemicellulose) would greatly increase potential revenues of a lignocellulose biorefinery...
  4. doi request reprint Constructing the electricity-carbohydrate-hydrogen cycle for a sustainability revolution
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Tech, 304 Seitz Hall, Blacksburg, Virginia 24061, USA
    Trends Biotechnol 30:301-6. 2012
    ....
  5. doi request reprint Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase
    Xinhao Ye
    Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia 24061, USA
    Mol Biosyst 8:1815-23. 2012
    ..3 to 24.6 min. The temperature optimum of CM3 was increased from 60 to 80 °C. These results suggested that a combination of rational design and random mutagenesis could have a solid basis for engineering large proteins...
  6. pmc Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis
    Chun You
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
    Appl Environ Microbiol 78:1593-5. 2012
    ..The DNA multimer generated by prolonged overlap extension PCR was directly transformed in Escherichia coli [e.g., TOP10, DH5α, JM109, and BL21(DE3)] and Bacillus subtilis for obtaining chimeric plasmids...
  7. pmc Enhanced microbial utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex
    Chun You
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA
    Appl Environ Microbiol 78:1437-44. 2012
    ..3- to 4.5-fold would help in the development of better consolidated bioprocessing microorganisms (e.g., B. subtilis) that can hydrolyze recalcitrant cellulose rapidly at low secretory cellulase levels...
  8. pmc Cell-free protein synthesis energized by slowly-metabolized maltodextrin
    Yiran Wang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
    BMC Biotechnol 9:58. 2009
    ..Cell-free protein synthesis (CFPS) is a rapid and high throughput technology for obtaining proteins from their genes. The primary energy source ATP is regenerated from the secondary energy source through substrate phosphorylation in CFPS...
  9. doi request reprint Substrate channeling and enzyme complexes for biotechnological applications
    Y H Percival Zhang
    Biological Systems Engineering Department, 210 A Seitz Hall, Virginia Tech, Blacksburg, VA 24061, USA
    Biotechnol Adv 29:715-25. 2011
    ....
  10. doi request reprint Biofuel production by in vitro synthetic enzymatic pathway biotransformation
    Y H Percival Zhang
    Biological Systems Engineering Department, 210 A Seitz Hall, Blacksburg, VA 24061, USA
    Curr Opin Biotechnol 21:663-9. 2010
    ..It is expected that high-yield SyPaB will be an important platform for producing low-cost biofuels and biochemicals...
  11. pmc Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration
    Yiran Wang
    Biological Systems Engineering Department, 210 A Seitz Hall, Virginia Polytechnic Institute and State University, Blacksburg, Virgina 24061, USA
    Microb Cell Fact 8:30. 2009
    ..abstract:..
  12. ncbi request reprint A functionally based model for hydrolysis of cellulose by fungal cellulase
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Biotechnol Bioeng 94:888-98. 2006
    ..Opportunities to further refine cellulase kinetic models are discussed, including parameters that would benefit from further study...
  13. ncbi request reprint Biosynthesis of radiolabeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars
    Y H P Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall 0303, Blacksburg, VA 24060, USA
    Appl Microbiol Biotechnol 70:123-9. 2006
    ..Results showed that the largest amount of radioactivity was cellobiose with lesser amounts of glucose, cellotriose, and cellotetraose, and an average DP of intracellular cellodextrins was ca. 2...
  14. doi request reprint Reviving the carbohydrate economy via multi-product lignocellulose biorefineries
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    J Ind Microbiol Biotechnol 35:367-75. 2008
    ..g., 30%) of transportation fuels through liquid biofuels, internal combustion engines in the short term, and would provide 100% transportation fuels by sugar-hydrogen-fuel cell systems in the long term...
  15. pmc High-yield hydrogen production from starch and water by a synthetic enzymatic pathway
    Y H Percival Zhang
    Biological Systems Engineering Department, Virginia Tech, Blacksburg, Virginia, United States of America
    PLoS ONE 2:e456. 2007
    ....
  16. ncbi request reprint Methodological analysis for determination of enzymatic digestibility of cellulosic materials
    Y H Percival Zhang
    Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Biotechnol Bioeng 96:188-94. 2007
    ..50%)...
  17. doi request reprint Efficient sugar release by the cellulose solvent-based lignocellulose fractionation technology and enzymatic cellulose hydrolysis
    Geoffrey Moxley
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall, Blacksburg, Virginia 24061, USA
    J Agric Food Chem 56:7885-90. 2008
    ..It was found that phosphoric acid only above a critical concentration (83%) with a sufficient reaction time can efficiently disrupt recalcitrant lignocellulose structures...
  18. ncbi request reprint Quantitative determination of cellulose accessibility to cellulase based on adsorption of a nonhydrolytic fusion protein containing CBM and GFP with its applications
    Jiong Hong
    Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061, USA
    Langmuir 23:12535-40. 2007
    ..Declining heterogeneous cellulose reactivity is significantly attributed to a loss of CAC where the easily hydrolyzed cellulose fraction is digested first...
  19. doi request reprint Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: heterologous expression, characterization, and synergy with family 48 cellobiohydrolase
    Xiao zhou Zhang
    Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Bioresour Technol 101:5534-8. 2010
    ..This study provided useful information for understanding of the cellulose hydrolysis mechanism of this cellulolytic bacterium with potential industrial importance...
  20. doi request reprint A highly active phosphoglucomutase from Clostridium thermocellum: cloning, purification, characterization and enhanced thermostability
    Y Wang
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
    J Appl Microbiol 108:39-46. 2010
    ..Discovery and utilization of highly active and thermostable phosphoglucomutase (PGM) would be vital for biocatalysis mediated by multiple enzymes, for example, high-yield production of enzymatic hydrogen...
  21. doi request reprint Bioseparation of recombinant cellulose-binding module-proteins by affinity adsorption on an ultra-high-capacity cellulosic adsorbent
    Jiong Hong
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall, Blacksburg, VA 24061, USA
    Anal Chim Acta 621:193-9. 2008
    ..Five recombinant proteins (CBM-protein), regardless of intercellular or periplasmic form, were purified successfully for demonstration purpose...
  22. doi request reprint Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage
    Jiong Hong
    Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210 A Seitz Hall, Blacksburg, VA 24061, USA
    J Chromatogr A 1194:150-4. 2008
    ..0 to 6.5. Four recombinant proteins (green fluorescence protein, phosphoglucomutase, cellobiose phosphorylase, and glucan phosphorylase) have been purified successfully...
  23. ncbi request reprint Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis
    Y H Percival Zhang
    Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Biomacromolecules 6:1510-5. 2005
    ..By contrast, the DP of Avicel remained nearly constant during hydrolysis. The specific enzymatic cellulose hydrolysis rate is 100-fold higher for PASC as compared to Avicel...