Verne Schirch

Summary

Affiliation: Virginia Commonwealth University
Country: USA

Publications

  1. ncbi request reprint Serine hydroxymethyltransferase revisited
    Verne Schirch
    Department of Biochemistry and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, VA 23219, USA
    Curr Opin Chem Biol 9:482-7. 2005
  2. pmc Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 284:30949-56. 2009
  3. pmc Pyridoxal 5'-phosphate is a slow tight binding inhibitor of E. coli pyridoxal kinase
    Mohini S Ghatge
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia, United States of America
    PLoS ONE 7:e41680. 2012
  4. doi request reprint Kinetic and structural studies of the role of the active site residue Asp235 of human pyridoxal kinase
    Amit K Gandhi
    Department of Medicinal Chemistry, School of Pharmacy and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, 800 East Leigh Street, Suite 212, Richmond VA 23219, USA
    Biochem Biophys Res Commun 381:12-5. 2009
  5. pmc Crystal Structure of human pyridoxal kinase: structural basis of M(+) and M(2+) activation
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    Protein Sci 16:2184-94. 2007
  6. pmc Crystal structure of pyridoxal kinase from the Escherichia coli pdxK gene: implications for the classification of pyridoxal kinases
    Martin K Safo
    Department of Medicinal Chemistry and Institute for Structural Biology and Drug Discovery, 800 E Leigh St, Virginia Commonwealth University, Richmond, VA 23219, USA
    J Bacteriol 188:4542-52. 2006
  7. pmc Crystal structures of human pyridoxal kinase in complex with the neurotoxins, ginkgotoxin and theophylline: insights into pyridoxal kinase inhibition
    Amit K Gandhi
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia, United States of America
    PLoS ONE 7:e40954. 2012
  8. ncbi request reprint Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme
    Martin K Safo
    Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    Acta Crystallogr D Biol Crystallogr 61:599-604. 2005
  9. pmc Crystal structure of the PdxY Protein from Escherichia coli
    Martin K Safo
    Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA 23219, USA
    J Bacteriol 186:8074-82. 2004
  10. pmc Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase
    Faik N Musayev
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, 800 E Leigh Street, Richmond, VA 23219, USA
    Protein Sci 12:1455-63. 2003

Collaborators

  • Martin K Safo
  • Emily S Boja
  • Tzu Fun Fu
  • Martino L di Salvo
  • Faik N Musayev
  • Amit K Gandhi
  • Mohini S Ghatge
  • Roberto Contestabile
  • Jigar V Desai
  • Tzu Ping Ko
  • Doletha M E Szebenyi
  • Rita Florio
  • Isabel N González
  • Alessia Parroni
  • Stefano Di Biase
  • Christina M Camara
  • Richmond Danso-Danquah
  • Aaron Sease
  • Mario A Saavedra
  • Alexina Haynes
  • Samuel O Aboagye
  • Ashwini Goswami
  • Sharyn Hunt
  • Francesco Bossa
  • Samanta Raboni

Detail Information

Publications17

  1. ncbi request reprint Serine hydroxymethyltransferase revisited
    Verne Schirch
    Department of Biochemistry and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, VA 23219, USA
    Curr Opin Chem Biol 9:482-7. 2005
    ..Molecular modeling revealed how a variety of substrates could be accommodated in the folate-independent cleavage of 3-hydroxyamino acids and shed light on the mechanism of this reaction...
  2. pmc Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 284:30949-56. 2009
    ..These results provide a molecular basis for the phenotype associated with the R229W mutation, as well as providing a foundation for understanding the pathophysiological consequences of pyridoxine 5'-phosphate oxidase mutations...
  3. pmc Pyridoxal 5'-phosphate is a slow tight binding inhibitor of E. coli pyridoxal kinase
    Mohini S Ghatge
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia, United States of America
    PLoS ONE 7:e41680. 2012
    ..These results open new perspectives on the mechanism of regulation and role of pyridoxal kinase in the Escherichia coli cell...
  4. doi request reprint Kinetic and structural studies of the role of the active site residue Asp235 of human pyridoxal kinase
    Amit K Gandhi
    Department of Medicinal Chemistry, School of Pharmacy and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, 800 East Leigh Street, Suite 212, Richmond VA 23219, USA
    Biochem Biophys Res Commun 381:12-5. 2009
    ..PLP.ATP complex could occur in the wild-type enzyme, consistent with severe MgATP substrate inhibition of PL kinase in the presence of PLP...
  5. pmc Crystal Structure of human pyridoxal kinase: structural basis of M(+) and M(2+) activation
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    Protein Sci 16:2184-94. 2007
    ....
  6. pmc Crystal structure of pyridoxal kinase from the Escherichia coli pdxK gene: implications for the classification of pyridoxal kinases
    Martin K Safo
    Department of Medicinal Chemistry and Institute for Structural Biology and Drug Discovery, 800 E Leigh St, Virginia Commonwealth University, Richmond, VA 23219, USA
    J Bacteriol 188:4542-52. 2006
    ..Both loop I and loop II are suggested to play significant roles in the functions of PL kinases...
  7. pmc Crystal structures of human pyridoxal kinase in complex with the neurotoxins, ginkgotoxin and theophylline: insights into pyridoxal kinase inhibition
    Amit K Gandhi
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia, United States of America
    PLoS ONE 7:e40954. 2012
    ..Such adverse effects may be prevented by administration of an appropriate form of vitamin B₆, or provide clues of how to modify these drugs to help reduce their hPL kinase inhibitory effects...
  8. ncbi request reprint Structure of Escherichia coli pyridoxine 5'-phosphate oxidase in a tetragonal crystal form: insights into the mechanistic pathway of the enzyme
    Martin K Safo
    Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    Acta Crystallogr D Biol Crystallogr 61:599-604. 2005
    ....
  9. pmc Crystal structure of the PdxY Protein from Escherichia coli
    Martin K Safo
    Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA 23219, USA
    J Bacteriol 186:8074-82. 2004
    ..The structure of PdxY also permitted an interpretation of work that was previously published about this enzyme...
  10. pmc Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase
    Faik N Musayev
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, 800 E Leigh Street, Richmond, VA 23219, USA
    Protein Sci 12:1455-63. 2003
    ..As observed with the E. coli enzyme, the human enzyme binds one molecule of pyridoxal 5'-phosphate tightly on each subunit...
  11. ncbi request reprint Location of the pteroylpolyglutamate-binding site on rabbit cytosolic serine hydroxymethyltransferase
    Tzu Fun Fu
    Department of Biochemistry and the Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond 23219 1570, USA
    J Biol Chem 278:2645-53. 2003
    ....
  12. ncbi request reprint Role of proline residues in the folding of serine hydroxymethyltransferase
    Tzu Fun Fu
    Department of Biochemistry, Institute of Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 278:31088-94. 2003
    ..The results are discussed in terms of the role of each Pro residue in maintaining the structure and function of SHMT and a possible role in pyridoxal 5'-phosphate addition to the apo-enzyme...
  13. ncbi request reprint Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases
    Martino L di Salvo
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita degli Studi di Roma, La Sapienza, Rome, Italy
    Protein Expr Purif 36:300-6. 2004
    ..We conclude that in vivo this kinase may have an alternate substrate involved in another metabolic pathway and that pyridoxal has only a poor secondary activity for this kinase...
  14. ncbi request reprint Serine hydroxymethyltransferase: role of glu75 and evidence that serine is cleaved by a retroaldol mechanism
    Doletha M E Szebenyi
    MacCHESS at Cornell High Energy Synchrotron Source, Cornell University, Ithaca, New York 14853, USA
    Biochemistry 43:6865-76. 2004
    ..This mechanism explains the greatly enhanced rate of serine cleavage in the presence of folate, and avoids some serious difficulties presented by the nucleophilic displacement mechanism involving breakage of the C3-OH bond...
  15. ncbi request reprint Structure and mechanism of Escherichia coli pyridoxine 5'-phosphate oxidase
    Martino L di Salvo
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita La Sapienza, P le Aldo Moro 5, 00185, Via degli Apuli 9, Rome, Italy
    Biochim Biophys Acta 1647:76-82. 2003
    ..The crystal structure and the stereospecificity studies suggest that the pair of electrons on C4' of the substrate are transferred to FMN as a hydride ion...
  16. ncbi request reprint Active site structure and stereospecificity of Escherichia coli pyridoxine-5'-phosphate oxidase
    Martino L di Salvo
    A Rossi Fanelli Dipartimento di Scienze Biochimiche, Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Universita La Sapienza, Rome, Italy
    J Mol Biol 315:385-97. 2002
    ..The crystal structure and the stereospecificity studies suggests that the pair of electrons on C4' of the substrate are transferred to FMN as a hydride ion...
  17. ncbi request reprint Properties of human and rabbit cytosolic serine hydroxymethyltransferase are changed by single nucleotide polymorphic mutations
    Tzu Fun Fu
    Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, College of Medicine, Tainan 701, Taiwan
    Arch Biochem Biophys 442:92-101. 2005
    ..Analysis of the structures of rabbit and human SHMT show how mutations at these two sites can result in the observed functional differences...