KEVIN MYLES

Summary

Affiliation: Virginia Polytechnic Institute and State University
Country: USA

Publications

  1. pmc Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes
    Kevin M Myles
    Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Proc Natl Acad Sci U S A 105:19938-43. 2008
  2. pmc Origins of alphavirus-derived small RNAs in mosquitoes
    Kevin M Myles
    Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061, USA
    RNA Biol 6:387-91. 2009
  3. pmc Effects of an opal termination codon preceding the nsP4 gene sequence in the O'Nyong-Nyong virus genome on Anopheles gambiae infectivity
    Kevin M Myles
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA
    J Virol 80:4992-7. 2006
  4. pmc A transgenic sensor strain for monitoring the RNAi pathway in the yellow fever mosquito, Aedes aegypti
    Zach N Adelman
    Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Insect Biochem Mol Biol 38:705-13. 2008
  5. pmc Identification and characterization of heat shock 70 genes in Aedes aegypti (Diptera: Culicidae)
    Tiffany L Gross
    Department of Entomology, Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    J Med Entomol 46:496-504. 2009
  6. pmc Accurate strand-specific quantification of viral RNA
    Nicole E Plaskon
    Fralin Life Science Institute, Department of Entomology, Virginia Tech, Blacksburg, Virginia, United States of America
    PLoS ONE 4:e7468. 2009
  7. pmc Deletions in the putative cell receptor-binding domain of Sindbis virus strain MRE16 E2 glycoprotein reduce midgut infectivity in Aedes aegypti
    Kevin M Myles
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80526, USA
    J Virol 77:8872-81. 2003
  8. ncbi request reprint Comparison of the transmission potential of two genetically distinct Sindbis viruses after oral infection of Aedes aegypti (Diptera: Culicidae)
    Kevin M Myles
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Med Entomol 41:95-106. 2004

Research Grants

  1. Arboviral suppressors of RNA interference
    KEVIN MYLES; Fiscal Year: 2007
  2. Modulation of arboviruses in mosquitoes
    Kevin M Myles; Fiscal Year: 2010

Collaborators

Detail Information

Publications8

  1. pmc Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes
    Kevin M Myles
    Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Proc Natl Acad Sci U S A 105:19938-43. 2008
    ..These results suggest that an exogenous siRNA pathway is essential to the survival of mosquitoes infected with alphaviruses and, thus, the maintenance of these viruses in nature...
  2. pmc Origins of alphavirus-derived small RNAs in mosquitoes
    Kevin M Myles
    Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061, USA
    RNA Biol 6:387-91. 2009
    ..Finally, we summarize evidence indicating that alphavirus double-stranded replicative intermediates trigger an exogenous-siRNA pathway in mosquitoes resulting in the biogenesis of virus-derived siRNAs...
  3. pmc Effects of an opal termination codon preceding the nsP4 gene sequence in the O'Nyong-Nyong virus genome on Anopheles gambiae infectivity
    Kevin M Myles
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA
    J Virol 80:4992-7. 2006
    ..Taken together, these results suggest that the primary fitness advantage provided to ONNV by the presence of an opal codon between nsP3 and nsP4 is related to mosquito infectivity...
  4. pmc A transgenic sensor strain for monitoring the RNAi pathway in the yellow fever mosquito, Aedes aegypti
    Zach N Adelman
    Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Insect Biochem Mol Biol 38:705-13. 2008
    ..This transgenic sensor strain can now be used to identify other components of the mosquito RNAi pathway and has the potential to be used in the identification of arboviral suppressors of RNAi...
  5. pmc Identification and characterization of heat shock 70 genes in Aedes aegypti (Diptera: Culicidae)
    Tiffany L Gross
    Department of Entomology, Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    J Med Entomol 46:496-504. 2009
    ..aegypti may serve as a general marker for stress. In addition, promoter sequences from AaHsp70 genes may be used to control the expression of transgenes in an inducible manner...
  6. pmc Accurate strand-specific quantification of viral RNA
    Nicole E Plaskon
    Fralin Life Science Institute, Department of Entomology, Virginia Tech, Blacksburg, Virginia, United States of America
    PLoS ONE 4:e7468. 2009
    ..Finally, we describe development, design and validation of ssqPCR assays for chikungunya virus (CHIKV), the recent cause of large outbreaks of disease in the Indian Ocean region...
  7. pmc Deletions in the putative cell receptor-binding domain of Sindbis virus strain MRE16 E2 glycoprotein reduce midgut infectivity in Aedes aegypti
    Kevin M Myles
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80526, USA
    J Virol 77:8872-81. 2003
    ..0 log(10) PFU/ml) in mosquitoes 4 days after infection by intrathoracic inoculation. These results suggest that the deleted portion of the E2 CRBD represents an important determinant of MRE16 virus midgut infectivity in A. aegypti...
  8. ncbi request reprint Comparison of the transmission potential of two genetically distinct Sindbis viruses after oral infection of Aedes aegypti (Diptera: Culicidae)
    Kevin M Myles
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Med Entomol 41:95-106. 2004
    ..MRE16 and TR339 viruses now represent a well-characterized model system for the further study of virus determinants of vector infection, particularly determinants affecting the midgut escape barrier in Ae. aegypti...

Research Grants5

  1. Arboviral suppressors of RNA interference
    KEVIN MYLES; Fiscal Year: 2007
    ..The identification of suppressors of RNA silencing encoded in mosquito-borne virus genomes would have enormous implications for understanding the transmission of arthropod-borne viral diseases. ..
  2. Modulation of arboviruses in mosquitoes
    Kevin M Myles; Fiscal Year: 2010
    ..This knowledge is vital to understanding and predicting how arboviral epidemics are initiated, spread, and maintained, and may ultimately form the basis of RNAi- based transgenic and field diagnostic approaches for disease control. ..