Rakesh C Kukreja

Summary

Affiliation: Virginia Commonwealth University
Country: USA

Publications

  1. pmc Anti-inflammatory and cardioprotective effects of tadalafil in diabetic mice
    Amit Varma
    Division of Cardiology, Department of Internal Medicine, VCU Pauley Heart Center, Virginia Commonwealth University, Richmond, Virginia, USA
    PLoS ONE 7:e45243. 2012
  2. pmc Sirtuin 1 (SIRT1) activation mediates sildenafil induced delayed cardioprotection against ischemia-reperfusion injury in mice
    Mona Shalwala
    VCU Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia, United States of America Touro College of Osteopathic Medicine, New York, New York, United States of America
    PLoS ONE 9:e86977. 2014
  3. ncbi request reprint Sildenafil and cardioprotection
    Rakesh C Kukreja
    Scientific Director, Pauley Heart Center, Professor of Medicine, Physiology, Biochemistry, and Emergency Medicine, Eric Lipman Professor of Cardiology, Division of Cardiology, Box 980204, Virginia Commonwealth University, 1101 East Marshall Street, Room 7 020D, Richmond, VA 23298, USA
    Curr Pharm Des 19:6842-7. 2013
  4. doi request reprint Cyclic guanosine monophosphate signaling and phosphodiesterase-5 inhibitors in cardioprotection
    Rakesh C Kukreja
    Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Am Coll Cardiol 59:1921-7. 2012
  5. pmc MicroRNAs: new players in cardiac injury and protection
    Rakesh C Kukreja
    Division of Cardiology, Virginia Commonwealth University, Richmond, VA 23298, USA
    Mol Pharmacol 80:558-64. 2011
  6. ncbi request reprint Essential role of mitochondrial Ca2+-activated and ATP-sensitive K+ channels in sildenafil-induced late cardioprotection
    Xiaoyin Wang
    Division of Cardiology, Department of Internal Medicine, Box 980204, Virginia Commonwealth University, 1101 East Mashall Street, Room 7 046, Richmond, VA 23298 0204, USA
    J Mol Cell Cardiol 44:105-13. 2008
  7. pmc Long-acting phosphodiesterase-5 inhibitor tadalafil attenuates doxorubicin-induced cardiomyopathy without interfering with chemotherapeutic effect
    Saisudha Koka
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, Virginia, USA
    J Pharmacol Exp Ther 334:1023-30. 2010
  8. doi request reprint Sildenafil (Viagra) attenuates ischemic cardiomyopathy and improves left ventricular function in mice
    Fadi N Salloum
    Division of Cardiology, Department of Internal, Virginia Commonwealth University, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 294:H1398-406. 2008
  9. pmc ERK phosphorylation mediates sildenafil-induced myocardial protection against ischemia-reperfusion injury in mice
    Anindita Das
    Division of Cardiology, Department of Internal Medicine, Box 980281, Virginia Commonwealth Univ Medical Center, 1101 East Marshall St, Rm 7 040, Richmond, VA 23298 0281, USA
    Am J Physiol Heart Circ Physiol 296:H1236-43. 2009
  10. ncbi request reprint Protein kinase C plays an essential role in sildenafil-induced cardioprotection in rabbits
    Anindita Das
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 286:H1455-60. 2004

Research Grants

Collaborators

Detail Information

Publications67

  1. pmc Anti-inflammatory and cardioprotective effects of tadalafil in diabetic mice
    Amit Varma
    Division of Cardiology, Department of Internal Medicine, VCU Pauley Heart Center, Virginia Commonwealth University, Richmond, Virginia, USA
    PLoS ONE 7:e45243. 2012
    ....
  2. pmc Sirtuin 1 (SIRT1) activation mediates sildenafil induced delayed cardioprotection against ischemia-reperfusion injury in mice
    Mona Shalwala
    VCU Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia, United States of America Touro College of Osteopathic Medicine, New York, New York, United States of America
    PLoS ONE 9:e86977. 2014
    ..SIRT1 is part of the class III Sirtuin family of histone deacetylases that deacetylates proteins involved in cellular stress response including those related to I-R injury...
  3. ncbi request reprint Sildenafil and cardioprotection
    Rakesh C Kukreja
    Scientific Director, Pauley Heart Center, Professor of Medicine, Physiology, Biochemistry, and Emergency Medicine, Eric Lipman Professor of Cardiology, Division of Cardiology, Box 980204, Virginia Commonwealth University, 1101 East Marshall Street, Room 7 020D, Richmond, VA 23298, USA
    Curr Pharm Des 19:6842-7. 2013
    ..Based on these studies, it appears that sildenafil and other PDE5 inhibitors hold promise for further development as novel drug therapies in cardioprotection. ..
  4. doi request reprint Cyclic guanosine monophosphate signaling and phosphodiesterase-5 inhibitors in cardioprotection
    Rakesh C Kukreja
    Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Am Coll Cardiol 59:1921-7. 2012
    ..In addition, PDE-5 inhibitors may prove to be innovative therapeutic agents for enhancing the chemosensitivity of doxorubicin while providing concurrent cardiac benefit...
  5. pmc MicroRNAs: new players in cardiac injury and protection
    Rakesh C Kukreja
    Division of Cardiology, Virginia Commonwealth University, Richmond, VA 23298, USA
    Mol Pharmacol 80:558-64. 2011
    ....
  6. ncbi request reprint Essential role of mitochondrial Ca2+-activated and ATP-sensitive K+ channels in sildenafil-induced late cardioprotection
    Xiaoyin Wang
    Division of Cardiology, Department of Internal Medicine, Box 980204, Virginia Commonwealth University, 1101 East Mashall Street, Room 7 046, Richmond, VA 23298 0204, USA
    J Mol Cell Cardiol 44:105-13. 2008
    ..The results suggest that activation of mitoK(Ca) and mitoK(ATP) are crucial for maintaining mitochondrial homeostasis and reducing cell death in sildenafil-induced preconditioning against ischemia-reperfusion injury...
  7. pmc Long-acting phosphodiesterase-5 inhibitor tadalafil attenuates doxorubicin-induced cardiomyopathy without interfering with chemotherapeutic effect
    Saisudha Koka
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, Virginia, USA
    J Pharmacol Exp Ther 334:1023-30. 2010
    ....
  8. doi request reprint Sildenafil (Viagra) attenuates ischemic cardiomyopathy and improves left ventricular function in mice
    Fadi N Salloum
    Division of Cardiology, Department of Internal, Virginia Commonwealth University, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 294:H1398-406. 2008
    ..05). Sildenafil attenuates ischemic cardiomyopathy in mice by limiting necrosis and apoptosis and by preserving left ventricular function possibly through a nitric oxide-dependent pathway...
  9. pmc ERK phosphorylation mediates sildenafil-induced myocardial protection against ischemia-reperfusion injury in mice
    Anindita Das
    Division of Cardiology, Department of Internal Medicine, Box 980281, Virginia Commonwealth Univ Medical Center, 1101 East Marshall St, Rm 7 040, Richmond, VA 23298 0281, USA
    Am J Physiol Heart Circ Physiol 296:H1236-43. 2009
    ..We conclude that these studies provide first direct evidence that PKG-dependent ERK phosphorylation is indispensable for the induction of eNOS/iNOS and Bcl-2 and the resulting cardioprotection by sildenafil...
  10. ncbi request reprint Protein kinase C plays an essential role in sildenafil-induced cardioprotection in rabbits
    Anindita Das
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 286:H1455-60. 2004
    ..However, no change in the PKC-beta and -epsilon isoforms was observed. These data provide direct evidence of an essential role of PKC, and potentially PKC-alpha, -, and -delta, in sildenafil-induced cardioprotection in the rabbit heart...
  11. doi request reprint Preconditioning by phosphodiesterase-5 inhibition improves therapeutic efficacy of adipose-derived stem cells following myocardial infarction in mice
    Nicholas N Hoke
    Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    Stem Cells 30:326-35. 2012
    ..We conclude that preconditioning by inhibition of PDE-5 can be a powerful novel approach to improve stem cell therapy following myocardial infarction...
  12. ncbi request reprint Pharmacological preconditioning with sildenafil: Basic mechanisms and clinical implications
    Rakesh C Kukreja
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, P O Box 980281, 1101 East Mashall Street, Room 7 046, Richmond, VA 23298 0281, USA
    Vascul Pharmacol 42:219-32. 2005
    ....
  13. pmc Sildenafil increases chemotherapeutic efficacy of doxorubicin in prostate cancer and ameliorates cardiac dysfunction
    Anindita Das
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Pauley Heart Center, Richmond, VA 23298, USA
    Proc Natl Acad Sci U S A 107:18202-7. 2010
    ..In conclusion, these results provide provocative evidence that sildenafil is both a powerful sensitizer of DOX-induced killing of prostate cancer while providing concurrent cardioprotective benefit...
  14. pmc Cinaciguat, a novel activator of soluble guanylate cyclase, protects against ischemia/reperfusion injury: role of hydrogen sulfide
    Fadi N Salloum
    Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, USA
    Am J Physiol Heart Circ Physiol 302:H1347-54. 2012
    ..In cardiomyocytes, cinaciguat (50 nM) reduced necrosis and apoptosis and increased H(2)S levels, which was abrogated by KT. Cinaciguat is a novel molecule to induce H(2)S generation and a powerful protection against I/R injury in heart...
  15. ncbi request reprint Protective effects of parecoxib, a cyclo-oxygenase-2 inhibitor, in postinfarction remodeling in the rat
    Stefania Straino
    VCU Pauley Heart Center, Virginia Commonwealth University, Richmond, VA 23298, USA
    J Cardiovasc Pharmacol 50:571-7. 2007
    ..The aim of the present study was to evaluate the effects of parecoxib, a selective COX-2 inhibitor, in an experimental AMI model in the rat...
  16. pmc Sildenafil and vardenafil but not nitroglycerin limit myocardial infarction through opening of mitochondrial K(ATP) channels when administered at reperfusion following ischemia in rabbits
    Fadi N Salloum
    Division of Cardiology, Department of Internal Medicine, Box 980281, Virginia Commonwealth University, 1101 East Mashall Street, Room 7 046, Richmond, VA 23298 0281, USA
    J Mol Cell Cardiol 42:453-8. 2007
    ..0+/-1.1% and 28.3+/-1.9%, respectively). Both sildenafil and vardenafil protect the ischemic myocardium against reperfusion injury through a mechanism dependent on mitochondrial K(ATP) channel opening...
  17. ncbi request reprint Phosphodiesterase-5 inhibitor sildenafil preconditions adult cardiac myocytes against necrosis and apoptosis. Essential role of nitric oxide signaling
    Anindita Das
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298 0281, USA
    J Biol Chem 280:12944-55. 2005
    ..The results may have possible therapeutic potential in preventing myocyte cell death following ischemia/reperfusion...
  18. doi request reprint Phosphodiesterase-5 inhibitor, tadalafil, protects against myocardial ischemia/reperfusion through protein-kinase g-dependent generation of hydrogen sulfide
    Fadi N Salloum
    Department of Internal Medicine, Virginia Commonwealth University, Richmond, 23298, USA
    Circulation 120:S31-6. 2009
    ..Additionally, we contemplated that cardioprotection with tadalafil is mediated by hydrogen sulfide (H(2)S) signaling in a PKG-dependent fashion...
  19. doi request reprint Hypoxia inducible factor-1 upregulates adiponectin in diabetic mouse hearts and attenuates post-ischemic injury
    Ramesh Natarajan
    Divisions of Pulmonary Disease and Critical Care Medicine, Virginia Commonwealth University Medical Center, Richmond, Virginia, USA
    J Cardiovasc Pharmacol 51:178-87. 2008
    ..Our studies suggest that local production of adiponectin by cardiomyocytes/microvascular endothelial cells may regulate cardiac function and indicate a novel strategy for protecting diabetic hearts from ischemia/reperfusion injury...
  20. ncbi request reprint Activation of hypoxia-inducible factor-1 via prolyl-4 hydoxylase-2 gene silencing attenuates acute inflammatory responses in postischemic myocardium
    Ramesh Natarajan
    Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Virginia Commonwealth University, PO Box 980050, Richmond, VA 23298 0050, USA
    Am J Physiol Heart Circ Physiol 293:H1571-80. 2007
    ..saline I/R, P < 0.001, n = 6). In conclusion, these results demonstrate for the first time that HIF-1 activation following infusion of siRNA to P4HA2 plays a key role in modulating I/R-associated cardiac inflammatory responses...
  21. doi request reprint Anakinra, a recombinant human interleukin-1 receptor antagonist, inhibits apoptosis in experimental acute myocardial infarction
    Antonio Abbate
    Division of Cardiology VCU Pauley Heart Center, Virginia Commonwealth University, 1200 E Broad St, West Hospital, 10th Floor, East Wing, Room 1041, PO Box 980281, Richmond, VA 23298 0281, USA
    Circulation 117:2670-83. 2008
    ..The aim of the present study was to test the impact of an exogenous recombinant human interleukin-1 receptor antagonist (anakinra) in experimental acute myocardial infarction...
  22. ncbi request reprint Sildenafil citrate (viagra) induces cardioprotective effects after ischemia/reperfusion injury in infant rabbits
    Yvonne A Bremer
    Division of Pediatric Cardiology, Department of Pediatrics, Virginia Commonwealth University Health System, Richmond, VA 23298, USA
    Pediatr Res 57:22-7. 2005
    ..05). For the first time, we have shown that sildenafil citrate promotes myocardial protection in infant rabbits as evidenced by postischemic preservation and elevation in LVCO and aortic VTI and reduction in infarct size...
  23. pmc Dietary inorganic nitrate alleviates doxorubicin cardiotoxicity: mechanisms and implications
    Lei Xi
    VCU Pauley Heart Center, Division of Cardiology, Virginia Commonwealth University, Richmond, VA 23298 0204, United States
    Nitric Oxide 26:274-84. 2012
    ..Future clinical trials in the cancer patients undergoing DOX chemotherapy are warranted to translate these experimental findings into an effective new therapy in preventing the DOX-induced cardiomyopathy...
  24. ncbi request reprint Adenosine A(1) receptor mediates delayed cardioprotective effect of sildenafil in mouse
    Fadi N Salloum
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA 23298 0281, USA
    J Mol Cell Cardiol 43:545-51. 2007
    ..These results suggest that A(1)AR activation following treatment with sildenafil plays an integral role in the signaling cascade responsible for delayed protection against global I/R injury...
  25. pmc Mammalian Target of Rapamycin (mTOR) Inhibition with Rapamycin Improves Cardiac Function in Type 2 Diabetic Mice: POTENTIAL ROLE OF ATTENUATED OXIDATIVE STRESS AND ALTERED CONTRACTILE PROTEIN EXPRESSION
    Anindita Das
    From the Pauley Heart Center, Division of Cardiology, Virginia Commonwealth University, Richmond, Virginia 23298
    J Biol Chem 289:4145-60. 2014
    ....
  26. ncbi request reprint Cardioprotection with phosphodiesterase-5 inhibition--a novel preconditioning strategy
    Rakesh C Kukreja
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Medical Center, Richmond, VA 23298, USA
    J Mol Cell Cardiol 36:165-73. 2004
    ....
  27. pmc Loss of myocardial ischemic postconditioning in adenosine A1 and bradykinin B2 receptors gene knockout mice
    Lei Xi
    Division of Cardiology, Box 980204, Virginia Commonwealth University, 1101 East Marshall Street, Room 7 042, Richmond, VA 23298 0204, USA
    Circulation 118:S32-7. 2008
    ..However, the exact subtypes of such receptors remain controversial or uninvestigated. To this context, the purpose of present study was to determine the definitive role of adenosine A(1) and bradykinin B(1) and B(2) receptors in PostC...
  28. pmc A novel role of microRNA in late preconditioning: upregulation of endothelial nitric oxide synthase and heat shock protein 70
    Chang Yin
    Division of Cardiology, Virginia Commonwealth University Medical Center, Richmond, VA 23298 0281, USA
    Circ Res 104:572-5. 2009
    ..We conclude that IPC-induced miRNAs trigger cardioprotection similar to the delayed phase of IPC, possibly through upregulating eNOS, HSP70, and the HSP70 transcription factor HSF-1...
  29. pmc Rapamycin protects against myocardial ischemia-reperfusion injury through JAK2-STAT3 signaling pathway
    Anindita Das
    Pauley Heart Center, Division of Cardiology, Virginia Commonwealth University, Richmond, VA, USA
    J Mol Cell Cardiol 53:858-69. 2012
    ..We propose that rapamycin is a novel and clinically relevant pharmacological strategy to target STAT3 activation for treatment of myocardial infarction...
  30. pmc Identification of protein targets underlying dietary nitrate-induced protection against doxorubicin cardiotoxicity
    Lei Xi
    VCU Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA 23298 0204, USA
    J Cell Mol Med 15:2512-24. 2011
    ..Up-regulation of Prx5 by nitrate may explain the observed enhancement of cardiac antioxidant defence by nitrate supplementation...
  31. pmc Protein kinase G-dependent cardioprotective mechanism of phosphodiesterase-5 inhibition involves phosphorylation of ERK and GSK3beta
    Anindita Das
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Biol Chem 283:29572-85. 2008
    ..Moreover, our results demonstrate that sildenafil activates a PKG-dependent novel signaling cascade that involves activation of ERK and inhibition of glycogen synthase kinase 3beta leading to cytoprotection...
  32. ncbi request reprint Vardenafil: a novel type 5 phosphodiesterase inhibitor reduces myocardial infarct size following ischemia/reperfusion injury via opening of mitochondrial K(ATP) channels in rabbits
    Fadi N Salloum
    Division of Cardiology, Department of Internal Medicine, Box 980281, Virginia Commonwealth University, 1101 East Marshall Street, Room 7 046, Richmond, VA 23298 0281, USA
    J Mol Cell Cardiol 40:405-11. 2006
    ..These results further support our hypothesis that the novel class of PDE-5 inhibitors induce protective effect in the ischemic heart, in addition to their well known clinical effects in the treatment of erectile dysfunction in men...
  33. ncbi request reprint Sildenafil induces delayed preconditioning through inducible nitric oxide synthase-dependent pathway in mouse heart
    Fadi Salloum
    Division of Cardiology, Box 980281, Virginia Commonwealth University, Richmond, VA 23298, USA
    Circ Res 92:595-7. 2003
    ..However, iNOS appears to be the primary isoform that mediates the robust cardioprotection...
  34. ncbi request reprint Phosphodiesterase-5 inhibition with sildenafil attenuates cardiomyocyte apoptosis and left ventricular dysfunction in a chronic model of doxorubicin cardiotoxicity
    Patrick W Fisher
    Department of Internal Medicine, Division of Cardiology, Virginia Commonwealth University Medical Center, Richmond 23298, USA
    Circulation 111:1601-10. 2005
    ..It is unclear whether sildenafil would provide similar protection from doxorubicin-induced cardiotoxicity...
  35. doi request reprint Phosphodiesterase-5 inhibitor tadalafil attenuates oxidative stress and protects against myocardial ischemia/reperfusion injury in type 2 diabetic mice
    Saisudha Koka
    Pauley Heart Center, Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, VA 23298 0204, USA
    Free Radic Biol Med 60:80-8. 2013
    ..In conclusion, these results demonstrate that chronic treatment with tadalafil attenuates oxidative stress and improves mitochondrial integrity while providing powerful cardioprotective effects in type 2 diabetes...
  36. ncbi request reprint Rapamycin confers preconditioning-like protection against ischemia-reperfusion injury in isolated mouse heart and cardiomyocytes
    Shakil Khan
    Department of Internal Medicine, Division of Cardiology, Virginia Commonwealth University Medical Center, Richmond, 23298, USA
    J Mol Cell Cardiol 41:256-64. 2006
    ..We propose that rapamycin may be a novel therapeutic strategy to limit infarction, apoptosis, and remodeling following I/R injury in the heart...
  37. pmc Mitigation of the progression of heart failure with sildenafil involves inhibition of RhoA/Rho-kinase pathway
    Vinh Q Chau
    Div of Cardiology, Box 980204, Virginia Commonwealth Univ, 1101 E Marshall St, Rm 7 020A, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 300:H2272-9. 2011
    ..Sildenafil may be a promising therapeutic tool for advanced HF in patients...
  38. pmc Left ventricular systolic dysfunction induced by ventricular ectopy: a novel model for premature ventricular contraction-induced cardiomyopathy
    Jose F Huizar
    McGuire VA Medical Center, Cardiology Division, and Virginia Commonwealth University, Richmond, VA, USA
    Circ Arrhythm Electrophysiol 4:543-9. 2011
    ..We developed a PVC-induced cardiomyopathy animal model using a novel premature pacing algorithm to assess timeframe and reversibility of this cardiomyopathy and examine the associated histopathologic abnormalities...
  39. ncbi request reprint HIF-1 activation attenuates postischemic myocardial injury: role for heme oxygenase-1 in modulating microvascular chemokine generation
    Ramzi Ockaili
    Div of Cardiology, Dept of Internal Medicine, Virginia Commonwealth Univ, PO Box 980050, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 289:H542-8. 2005
    ..Thus our data show that HIF-1 activation induces substantial HO-1 expression that is associated with attenuated proinflammatory chemokine production by microvascular endothelium in vitro and in vivo...
  40. ncbi request reprint Cobalt chloride induces delayed cardiac preconditioning in mice through selective activation of HIF-1alpha and AP-1 and iNOS signaling
    Lei Xi
    Division of Cardiology, Box 980281, Virginia Commonwealth Univ, Richmond, VA 23298 0281, USA
    Am J Physiol Heart Circ Physiol 287:H2369-75. 2004
    ..The delayed protective effect of CoCl2 is achieved through a distinctive signaling mechanism involving HIF-1alpha, AP-1, and iNOS but independent of NF-kappaB activation...
  41. ncbi request reprint Improvement of cardiac function with parecoxib, a cyclo-oxygenase-2 inhibitor, in a rat model of ischemic heart failure
    Antonio Abbate
    Virginia Commonwealth University Pauley Heart Center, Richmond, VA 23233, USA
    J Cardiovasc Pharmacol 49:416-8. 2007
    ..To assess changes in cardiac function in animals with ischemic congestive heart failure (CHF) treated with a selective cyclo-oxygenase-2 (COX-2) inhibitor...
  42. pmc Induction of MicroRNA-21 With Exogenous Hydrogen Sulfide Attenuates Myocardial Ischemic and Inflammatory Injury in Mice
    Stefano Toldo
    From the Pauley Heart Center, Division of Cardiology, Department of Internal Medicine S T, A D, E M, V Q C, C M, D D, A s, B W V T, C Y, R A O, N V, R C K, A A, F N S and Department of Biostatistics N D M, Virginia Commonwealth University, Richmond
    Circ Cardiovasc Genet 7:311-20. 2014
    ....
  43. pmc Right ventricular cardiomyocyte apoptosis in patients with acute myocardial infarction of the left ventricular wall
    Antonio Abbate
    Virginia Commonwealth University, Pauley Heart Center, Richmond, Virginia, USA
    Am J Cardiol 102:658-62. 2008
    ..91, p = 0.002 for RV free wall thickness). In conclusion, patients with cardiac remodeling after AMI had a significant increase in RV apoptosis even when ischemic involvement of the RV wall was not apparent...
  44. ncbi request reprint Hypoxia inducible factor-1 activation by prolyl 4-hydroxylase-2 gene silencing attenuates myocardial ischemia reperfusion injury
    Ramesh Natarajan
    Division of Pulmonary Disease and Critical Care Medicine, Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, VA, USA
    Circ Res 98:133-40. 2006
    ..Normoxic activation of HIF-1 in hearts following in vivo PHD2 siRNA administration attenuates reperfusion injury via an iNOS-dependent pathway...
  45. ncbi request reprint Opening of Ca2+-activated K+ channels triggers early and delayed preconditioning against I/R injury independent of NOS in mice
    Xiaoyin Wang
    Division of Cardiology, Box 980281, Virginia Commonwealth Univ, Richmond, VA 23298 0281, USA
    Am J Physiol Heart Circ Physiol 287:H2070-7. 2004
    ..We conclude that opening of KCa channels leads to both early and delayed preconditioning effects through a mechanism that is independent of nitric oxide...
  46. ncbi request reprint Silencing heat shock factor 1 by small interfering RNA abrogates heat shock-induced cardioprotection against ischemia-reperfusion injury in mice
    Chang Yin
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, Richmond, VA 23298 0281, USA
    J Mol Cell Cardiol 39:681-9. 2005
    ..For the first time, we have applied siRNA technique in the field of myocardial preconditioning to demonstrate HSF1 activation as an essential step in WBH preconditioning against cardiac ischemia-reperfusion injury...
  47. ncbi request reprint Cyclic GMP-dependent protein kinase Ialpha attenuates necrosis and apoptosis following ischemia/reoxygenation in adult cardiomyocyte
    Anindita Das
    Department of Internal Medicine, Division of Cardiology, Virginia Commonwealth University Medical Center, Richmond, VA 23298, USA
    J Biol Chem 281:38644-52. 2006
    ....
  48. doi request reprint Chronic treatment with long acting phosphodiesterase-5 inhibitor tadalafil alters proteomic changes associated with cytoskeletal rearrangement and redox regulation in Type 2 diabetic hearts
    Saisudha Koka
    Division of Cardiology, Department of Internal Medicine, VCU Pauley Heart Center, Virginia Commonwealth University Medical Center, 1101 East Marshall Street, Room 7 020D, Box 980204, Richmond, VA 23298 0204, USA
    Basic Res Cardiol 107:249. 2012
    ..Chronic treatment with tadalafil in db/db mice modulates proteins involved in cytoskeletal rearrangement and redox signaling of the heart, which may explain the beneficial effects of PDE-5 inhibition in diabetes...
  49. pmc Endogenous microRNAs induced by heat-shock reduce myocardial infarction following ischemia-reperfusion in mice
    Chang Yin
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University Medical Center, 1101 E Marshall St Sanger Hall, Box 980281, Richmond, VA 23298 0281, USA
    FEBS Lett 582:4137-42. 2008
    ..Overall, these studies for the first time provide evidence for the potential role of endogenously synthesized miRNA's in cardioprotection following I/R injury...
  50. pmc Type 2 diabetic obese db/db mice are refractory to myocardial ischaemic post-conditioning in vivo: potential role for Hsp20, F1-ATPase δ and Echs1
    Shu Guang Zhu
    Division of Cardiology, Department of Internal Medicine, VCU Pauley Heart Center, Virginia Commonwealth University, Richmond, VA 23298 0204, USA
    J Cell Mol Med 16:950-8. 2012
    ..The potential protein targets for the loss of PostC may include F(1)-ATPase γ, Echs1 and Hsp20 that could regulate cellular ATP consumption/production and defense response to ischaemic stress...
  51. ncbi request reprint Dietary nitrate supplementation protects against Doxorubicin-induced cardiomyopathy by improving mitochondrial function
    Shu Guang Zhu
    VCU Pauley Heart Center, Division of Cardiology, Virginia Commonwealth University, Richmond, Virginia 23298 0204, USA
    J Am Coll Cardiol 57:2181-9. 2011
    ..The aim of this study was to test the hypothesis that long-term dietary nitrate supplementation protects against doxorubicin-induced cardiomyopathy by improving ventricular function and reducing mitochondrial respiratory chain damage...
  52. pmc Emerging new uses of phosphodiesterase-5 inhibitors in cardiovascular diseases
    Rakesh C Kukreja
    Department of Internal Medicine, Division of Cardiology, Virginia Commonwealth University Pauley Heart Center, Richmond, Virginia, USA
    Exp Clin Cardiol 16:e30-5. 2011
    ..Future carefully controlled clinical trials would hopefully expedite their expanding therapeutic use in patients with cardiovascular disease...
  53. ncbi request reprint Hypercholesterolemia enhances tolerance to lethal systemic hypoxia in middle-aged mice: possible role of VEGF downregulation in brain
    Lei Xi
    Division of Cardiology, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298, USA
    Mol Cell Biochem 291:205-11. 2006
    ..This paradoxical protective effect of HCL was associated with a concomitant downregulation of cerebral VEGF expression, which could potentially blunt the hypoxia-triggered and VEGF-mediated pathophysiological events leading to death...
  54. pmc Mitochondrial-targeted Signal transducer and activator of transcription 3 (STAT3) protects against ischemia-induced changes in the electron transport chain and the generation of reactive oxygen species
    Karol Szczepanek
    Department of Biochemistry and Molecular Biology, Division of Cardiology, Pauley Heart Center, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Biol Chem 286:29610-20. 2011
    ..Given the caveat of STAT3 overexpression, these results suggest a novel protective mechanism mediated by mitochondrial STAT3 that is independent of its canonical activity as a nuclear transcription factor...
  55. pmc Curcumin prevents cardiac remodeling secondary to chronic renal failure through deactivation of hypertrophic signaling in rats
    Siddhartha S Ghosh
    Department of Internal Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 299:H975-84. 2010
    ..Considering the safety of curcumin, these studies should facilitate future clinical trials in suppressing hypertrophy in patients with CRF...
  56. pmc Phosphodiesterase 5 inhibitors enhance chemotherapy killing in gastrointestinal/genitourinary cancer cells
    Laurence Booth
    Departments of Biochemistry and Molecular Biology L B, J L R, N C, A C, P D, Cardiology D E D, A D, R C K, Medicine S G, A P, Human and Molecular Genetics P B F, Virginia Commonwealth University, Richmond, Virginia
    Mol Pharmacol 85:408-19. 2014
    ..Collectively our data demonstrate that the combination of PDE5 inhibitors with standard of care chemotherapy agents for gastrointestinal/genitourinary cancers represents a novel modality. ..
  57. doi request reprint Role of microRNAs in cardiac preconditioning
    Fadi N Salloum
    Division of Cardiology, Pauley Heart Center, Department of Internal Medicine, Virginia Commonwealth University, 1101 East Marshall St, Richmond, VA 23298, USA
    J Cardiovasc Pharmacol 56:581-8. 2010
    ..We believe that identification and targeted delivery of miR(s) in the heart could have an immense therapeutic potential in reducing myocardial infarction in patients suffering from heart disease...
  58. pmc Chronic inhibition of phosphodiesterase 5 with tadalafil attenuates mitochondrial dysfunction in type 2 diabetic hearts: potential role of NO/SIRT1/PGC-1α signaling
    Saisudha Koka
    Division of Cardiology, Department of Internal Medicine, Pauley Heart Center, Virginia Commonwealth University, Richmond, Virginia
    Am J Physiol Heart Circ Physiol 306:H1558-68. 2014
    ..In conclusion, chronic treatment with tadalafil activates NO-induced SIRT1-PGC-1α signaling and attenuates mitochondrial dysfunction in type 2 diabetic hearts. ..
  59. pmc Krüppel-like factor 2 is required for normal mouse cardiac development
    Aditi R Chiplunkar
    Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, Virginia, United States of America
    PLoS ONE 8:e54891. 2013
    ..In conclusion, KLF2-/- heart phenotypes are genetic background-dependent. KLF2 plays a role in EMT through its regulation of important cardiovascular genes...
  60. pmc A simple and sensitive HPLC fluorescence method for determination of tadalafil in mouse plasma
    Christine A Farthing
    Department of Internal Medicine, Division of Nephrology, Clinical Pharmacology and Hypertension, Virginia Commonwealth University, Richmond, VA 23298, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 878:2891-5. 2010
    ..The method was utilized on mouse plasma samples from research evaluating the potential cardioprotective effects of tadalafil on mouse heart tissue exposed to doxorubicin, a chemotherapeutic drug with reported cardiotoxic effects...
  61. doi request reprint Cardiac regenerative potential of adipose tissue-derived stem cells
    N N Hoke
    Department of Internal Medicine, Division of Cardiology, Virginia Commonwealth University, 1101 E Marshall Street, Richmond, VA 23298 0281, USA
    Acta Physiol Hung 96:251-65. 2009
    ..The aim of this review is to discuss the potential therapeutic benefits of adipose tissue-derived stem cells in improving cardiac function post-injury...
  62. ncbi request reprint Alpha-adrenergic receptor stimulation produces late preconditioning through inducible nitric oxide synthase in mouse heart
    M Isabel Tejero-Taldo
    Division of Cardiology, Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Mol Cell Cardiol 34:185-95. 2002
    ..Enhanced iNOS expression concomitant with increased NO synthesis, as well as pharmacological blockade and absence of cardioprotection in iNOS knockout mice suggests an essential role of NO in phenylephrine triggered late PC...
  63. ncbi request reprint Late preconditioning elicited by activation of adenosine A(3) receptor in heart: role of NF- kappa B, iNOS and mitochondrial K(ATP) channel
    Ting C Zhao
    Division of Cardiology, Medical College of Virginia, Richmond, VA 23298, USA
    J Mol Cell Cardiol 34:263-77. 2002
    ..Selective activation of A(3)AR with IB-MECA can be used to trigger long-lasting ischemic protection in the heart...
  64. ncbi request reprint Sildenafil (Viagra) induces powerful cardioprotective effect via opening of mitochondrial K(ATP) channels in rabbits
    Ramzi Ockaili
    Division of Cardiology, Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    Am J Physiol Heart Circ Physiol 283:H1263-9. 2002
    ..For the first time, we demonstrate that sildenafil induces acute and delayed protective effects against ischemia-reperfusion injury, which are mediated by opening of mitochondrial K(ATP) channels...
  65. ncbi request reprint Protein kinase C-delta mediates adenosine A3 receptor-induced delayed cardioprotection in mouse
    Ting Cun Zhao
    Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA
    Am J Physiol Heart Circ Physiol 285:H434-41. 2003
    ..Furthermore, IB-MECA-induced increase in nuclear factor-kappaB binding was diminished by CHE. These results provide direct evidence of an essential role of PKC, and more specifically, PKC-delta in A3AR-induced delayed cardioprotection...
  66. doi request reprint cis-3, 4', 5-Trimethoxy-3'-aminostilbene disrupts tumor vascular perfusion without damaging normal organ perfusion
    David Durrant
    Department of Internal Medicine, Massey Cancer Center, Virginia Commonwealth University, 1101 E Marshall Street, Richmond, VA 23298, USA
    Cancer Chemother Pharmacol 63:191-200. 2009
    ..Here we investigate cis-3, 4', 5-trimethoxy-3'-aminostilbene (stilbene 5c) in its effect on tumor vascular perfusion, pharmacokinetics, toxicity and therapeutic efficacy in a mouse xenograft model...
  67. ncbi request reprint Evidence that NOS2 acts as a trigger and mediator of late preconditioning induced by acute systemic hypoxia
    Lei Xi
    Division of Cardiology, Department of Internal Medicine, Virginia Commonwealth University, Richmond, Virginia 23298 0281, USA
    Am J Physiol Heart Circ Physiol 283:H5-12. 2002
    ..05), whereas endothelial NOS (NOS3) or COX-2 remained unchanged. We conclude that acute SH induces delayed cardioprotection, which is triggered and mediated by NOS2, but not by NOS3 or COX-2...

Research Grants2

  1. CARDIOPROTECTIVE EFFECTS OF PDE-5 INHIBITORS
    Rakesh C Kukreja; Fiscal Year: 2010
    ..Moreover, our studies will help understand the molecular basis of the protection with these drugs. ..
  2. Cardioprotective Signaling following Phosphodiesterase-5 Inhibition
    Rakesh C Kukreja; Fiscal Year: 2010
    ..In addition, our investigations will open up another innovative gene silencing option to treat AMI and ventricular remodeling in patients. ..