Ana Espinel-Ingroff

Summary

Affiliation: Virginia Commonwealth University
Country: USA

Publications

  1. doi request reprint In vitro activity of echinocandins against non-Candida albicans: is echinocandin antifungal activity the same?
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, VA, USA
    Enferm Infecc Microbiol Clin 29:3-9. 2011
  2. pmc Multicenter study of isavuconazole MIC distributions and epidemiological cutoff values for Aspergillus spp. for the CLSI M38-A2 broth microdilution method
    A Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    Antimicrob Agents Chemother 57:3823-8. 2013
  3. pmc Cryptococcus neoformans-Cryptococcus gattii species complex: an international study of wild-type susceptibility endpoint distributions and epidemiological cutoff values for fluconazole, itraconazole, posaconazole, and voriconazole
    A Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    Antimicrob Agents Chemother 56:5898-906. 2012
  4. pmc Correlation of Neo-Sensitabs tablet diffusion assay results on three different agar media with CLSI broth microdilution M27-A2 and disk diffusion M44-A results for testing susceptibilities of Candida spp. and Cryptococcus neoformans to amphotericin B, cas
    A Espinel-Ingroff
    Division of Infectious Diseases, VCU Medical Center, 1101 Marshal St, Sanger Hall, Room 7049, Richmond, VA 23298 0049, USA
    J Clin Microbiol 45:858-64. 2007
  5. pmc Multicenter evaluation of a new disk agar diffusion method for susceptibility testing of filamentous fungi with voriconazole, posaconazole, itraconazole, amphotericin B, and caspofungin
    A Espinel-Ingroff
    Virginia Commonwealth University Medical Center, Richmond, VA 23298 0049, USA
    J Clin Microbiol 45:1811-20. 2007
  6. pmc Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds
    A Espinel-Ingroff
    VCU Medical Center, 1101 E Marshall Street, Sanger Hall, Room 7 049, P O Box 980049, Richmond, VA 23298 0049, USA
    J Clin Microbiol 45:2180-2. 2007
  7. ncbi request reprint Mechanisms of resistance to antifungal agents: yeasts and filamentous fungi
    Ana Espinel-Ingroff
    VCU Medical Center, Department of Medicine, Division of Infectious Diseases, Richmond, VA 23298 0049, USA
    Rev Iberoam Micol 25:101-6. 2008
  8. doi request reprint Novel antifungal agents, targets or therapeutic strategies for the treatment of invasive fungal diseases: a review of the literature (2005-2009)
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, VA, USA
    Rev Iberoam Micol 26:15-22. 2009
  9. pmc Comparison of 24-hour and 48-hour voriconazole MICs as determined by the Clinical and Laboratory Standards Institute broth microdilution method (M27-A3 document) in three laboratories: results obtained with 2,162 clinical isolates of Candida spp. and othe
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    J Clin Microbiol 47:2766-71. 2009
  10. pmc Comparison of anidulafungin MICs determined by the clinical and laboratory standards institute broth microdilution method (M27-A3 document) and Etest for Candida species isolates
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    Antimicrob Agents Chemother 54:1347-50. 2010

Detail Information

Publications34

  1. doi request reprint In vitro activity of echinocandins against non-Candida albicans: is echinocandin antifungal activity the same?
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, VA, USA
    Enferm Infecc Microbiol Clin 29:3-9. 2011
    ..different parameters or measurements of in vitro potency were evaluated. The focus of the review is the non-Candida albicans species...
  2. pmc Multicenter study of isavuconazole MIC distributions and epidemiological cutoff values for Aspergillus spp. for the CLSI M38-A2 broth microdilution method
    A Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    Antimicrob Agents Chemother 57:3823-8. 2013
    ..These ECVs may aid in detecting non-WT isolates with reduced susceptibility to isavuconazole due to cyp51A (an A. fumigatus species complex resistance mechanism among the triazoles) or other mutations. ..
  3. pmc Cryptococcus neoformans-Cryptococcus gattii species complex: an international study of wild-type susceptibility endpoint distributions and epidemiological cutoff values for fluconazole, itraconazole, posaconazole, and voriconazole
    A Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    Antimicrob Agents Chemother 56:5898-906. 2012
    ..In the absence of clinical breakpoints, our ECVs may aid in the detection of isolates with acquired resistance mechanisms and should be listed in the revised CLSI M27-A3 and CLSI M27-S3 documents...
  4. pmc Correlation of Neo-Sensitabs tablet diffusion assay results on three different agar media with CLSI broth microdilution M27-A2 and disk diffusion M44-A results for testing susceptibilities of Candida spp. and Cryptococcus neoformans to amphotericin B, cas
    A Espinel-Ingroff
    Division of Infectious Diseases, VCU Medical Center, 1101 Marshal St, Sanger Hall, Room 7049, Richmond, VA 23298 0049, USA
    J Clin Microbiol 45:858-64. 2007
    ..These data suggest the potential value of the Neo-Sensitabs assay for testing at least four of the five agents against yeasts evaluated in the clinical laboratory...
  5. pmc Multicenter evaluation of a new disk agar diffusion method for susceptibility testing of filamentous fungi with voriconazole, posaconazole, itraconazole, amphotericin B, and caspofungin
    A Espinel-Ingroff
    Virginia Commonwealth University Medical Center, Richmond, VA 23298 0049, USA
    J Clin Microbiol 45:1811-20. 2007
    ....
  6. pmc Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds
    A Espinel-Ingroff
    VCU Medical Center, 1101 E Marshall Street, Sanger Hall, Room 7 049, P O Box 980049, Richmond, VA 23298 0049, USA
    J Clin Microbiol 45:2180-2. 2007
    ..apiospermum and 96.7 to 97.5% at 48 h (MICs, >or=32 microg/ml) for both Fusarium isolates. Introduction of these identified optimum testing conditions for anidulafungin into future versions of the M38 document is warranted...
  7. ncbi request reprint Mechanisms of resistance to antifungal agents: yeasts and filamentous fungi
    Ana Espinel-Ingroff
    VCU Medical Center, Department of Medicine, Division of Infectious Diseases, Richmond, VA 23298 0049, USA
    Rev Iberoam Micol 25:101-6. 2008
    ..This article provides a review of reported resistance molecular mechanisms to antifungal agents since 2005; previous related reviews are also listed...
  8. doi request reprint Novel antifungal agents, targets or therapeutic strategies for the treatment of invasive fungal diseases: a review of the literature (2005-2009)
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, VA, USA
    Rev Iberoam Micol 26:15-22. 2009
    ..Despite aggressive treatment with new or more established licensed antifungal agents, these infections are an important cause of morbidity and mortality, especially in immunocompromised patients...
  9. pmc Comparison of 24-hour and 48-hour voriconazole MICs as determined by the Clinical and Laboratory Standards Institute broth microdilution method (M27-A3 document) in three laboratories: results obtained with 2,162 clinical isolates of Candida spp. and othe
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    J Clin Microbiol 47:2766-71. 2009
    ..Either in vivo versus in vitro correlations or the determination of resistance mechanisms should be investigated...
  10. pmc Comparison of anidulafungin MICs determined by the clinical and laboratory standards institute broth microdilution method (M27-A3 document) and Etest for Candida species isolates
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, Virginia, USA
    Antimicrob Agents Chemother 54:1347-50. 2010
    ..guilliermondii (54.4 to 82.4% essential and categorical agreements) but is more suitable for C. albicans, C. glabrata, C. krusei, and C. tropicalis (87.9 to 100% categorical agreement)...
  11. pmc Wild-type MIC distributions and epidemiological cutoff values for the triazoles and six Aspergillus spp. for the CLSI broth microdilution method (M38-A2 document)
    A Espinel-Ingroff
    VCU Medical Center, 3804 Dover Rd, Richmond, VA 23221, USA
    J Clin Microbiol 48:3251-7. 2010
    ..These ECVs should be considered for inclusion in the future CLSI M38-A2 document revision...
  12. pmc Comparison of three commercial assays and a modified disk diffusion assay with two broth microdilution reference assays for testing zygomycetes, Aspergillus spp., Candida spp., and Cryptococcus neoformans with posaconazole and amphotericin B
    A Espinel-Ingroff
    Division of Infectious Diseases, VCU Medical Center, 1101 East Marshall St, Sanger Hall Room 7 049, Richmond, VA 23298 0049, USA
    J Clin Microbiol 44:3616-22. 2006
    ..These data suggest the potential value of these alternative broth dilution and agar diffusion methods for testing posaconazole and amphotericin B in the clinical laboratory against the species evaluated...
  13. doi request reprint Activities of voriconazole, itraconazole and amphotericin B in vitro against 590 moulds from 323 patients in the voriconazole Phase III clinical studies
    Ana Espinel-Ingroff
    Virginia Commonwealth University, Medical Centre, Richmond, VA, USA
    J Antimicrob Chemother 61:616-20. 2008
    ..Fungal pathogens from the voriconazole trials were identified and tested for susceptibility at two reference laboratories...
  14. pmc Evaluation of broth microdilution testing parameters and agar diffusion Etest procedure for testing susceptibilities of Aspergillus spp. to caspofungin acetate (MK-0991)
    Ana Espinel-Ingroff
    Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Clin Microbiol 41:403-9. 2003
    ..Future in vitro correlations with in vivo outcome of both microdilution and Etest procedures may detect more-relevant testing conditions...
  15. pmc E-test method for testing susceptibilities of Aspergillus spp. to the new triazoles voriconazole and posaconazole and to established antifungal agents: comparison with NCCLS broth microdilution method
    Ana Espinel-Ingroff
    Division of Infectious Diseases, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298 0049, USA
    J Clin Microbiol 40:2101-7. 2002
    ..The utility of in vitro results of either the E-test or the NCCLS broth microdilution (M38-P) method for Aspergillus spp. needs to be established in clinical trials...
  16. doi request reprint Comparison of micafungin MICs as determined by the Clinical and Laboratory Standards Institute broth microdilution method (M27-A3 document) and Etest for Candida spp. isolates
    Ana Espinel-Ingroff
    VCU Medical Center, Richmond, VA 23298, USA
    Diagn Microbiol Infect Dis 70:54-9. 2011
    ..2% and according to ECVs 97.3%). However, the number of resistant isolates was small, so further evaluations are needed with a higher number of such isolates including more resistant or those with known mechanisms of resistance (non-WT)...
  17. ncbi request reprint Utility of mould susceptibility testing
    Ana Espinel-Ingroff
    Medical College of Virginia Commonwealth, Richmond, Virginia 23298 0049, USA
    Curr Opin Infect Dis 16:527-32. 2003
    ..This paper reviews the available methods for antifungal susceptibility testing of moulds as well as the scant data that have been published since 2002 regarding the clinical implications of in-vitro testing...
  18. ncbi request reprint In vitro antifungal activities of anidulafungin and micafungin, licensed agents and the investigational triazole posaconazole as determined by NCCLS methods for 12,052 fungal isolates: review of the literature
    Ana Espinel-Ingroff
    Division of Infectious Diseases, VCU Medical Center, P O Box 49, Richmond, Virginia 23298 0049, USA
    Rev Iberoam Micol 20:121-36. 2003
    ..In vitro/in vivo correlations should aid in the interpretation of these results, but standard testing conditions are needed for the echinocandins, especially for mould testing, to obtain reliable results...
  19. ncbi request reprint Minimum fungicidal concentrations of amphotericin B for bloodstream Candida species
    Emilia Cantón
    Unidad de Microbiología Experimental Centro de Investigación, Hospital Universitario La Fe, 46009, Valencia, Spain
    Diagn Microbiol Infect Dis 45:203-6. 2003
    ..MFCs for C.tropicalis and C. glabrata were > or =2 microg/ml. By this more stringent method we found substantial differences from those previously reported between amphotericin B MIC and MFCs for Candida spp...
  20. ncbi request reprint Antifungal activity of the echinocandin anidulafungin (VER002, LY-303366) against yeast pathogens: a comparative study with M27-A microdilution method
    María Pilar Arévalo
    Catedra de Medicina Preventiva y Salud Publica, Facultad de Medicina, Universidad de La Laguna, 38071 La Laguna, Tenerife, Spain
    J Antimicrob Chemother 51:163-6. 2003
    ..The differences were not significant for the other species evaluated...
  21. ncbi request reprint Voriconazole treatment for less-common, emerging, or refractory fungal infections
    John R Perfect
    Department of Medicine and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Clin Infect Dis 36:1122-31. 2003
    ..Voriconazole is an effective and well-tolerated treatment for refractory or less-common invasive fungal infections...
  22. pmc Successful outcome of disseminated Fusarium infection with skin localization treated with voriconazole and amphotericin B-lipid complex in a patient with acute leukemia
    Isabelle Durand-Joly
    Parasitologie Mycologie, Faculte de Medecine, CHRU de Lille, Lille, France
    J Clin Microbiol 41:4898-900. 2003
    ..The infection was refractory to amphotericin B-lipid complex alone but responded successfully when voriconazole was added...
  23. doi request reprint Comparison of posaconazole and voriconazole in vitro killing against Candida krusei
    Emilia Cantón
    Unidad de Microbiologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, 46009 Valencia, Spain
    Diagn Microbiol Infect Dis 62:177-81. 2008
    ..3 h (32 mg/L voriconazole). By time kill, a 99% killing rate was not reached by either agent. Both methods demonstrated that posaconazole (more active and greater killing rate) and voriconazole have fungicidal activity against C. krusei...
  24. ncbi request reprint Comparative evaluation of ATB Fungus 2 and Sensititre YeastOne panels for testing in vitro Candida antifungal susceptibility
    Elena Eraso
    Laboratorio de Micologia Medica, Departamento de Inmunologia, Microbiologia y Parasitologia, Facultad de Medicina y Odontologia, Universidad del Pais Vasco, Bilbao, Spain
    Rev Iberoam Micol 25:3-6. 2008
    ..9-75.9%). This easy to perform method could be an alternative for routine use in the clinical microbiology laboratory for susceptibility testing of common Candida spp...
  25. pmc Comparison of two probes for testing susceptibilities of pathogenic yeasts to voriconazole, itraconazole, and caspofungin by flow cytometry
    Cidalia Pina-Vaz
    Department of Microbiology, Porto School of Medicine, Alameda Prof Hernani Monteiro, Porto, Portugal
    J Clin Microbiol 43:4674-9. 2005
    ..The use of FUN-1 appears to be an excellent fast and reliable alternative to the classical dilution method for determining the susceptibility of Candida spp. and C. neoformans to these three antifungal agents...
  26. pmc Killing kinetics of caspofungin, micafungin, and amphotericin B against Candida guilliermondii
    Emilia Cantón
    Unidad de Microbiología Experimental Centro de Investigación, Hospital Universitario La Fe, 46009 Valencia, Spain
    Antimicrob Agents Chemother 50:2829-32. 2006
    ..AMB was the only agent with consistent killing activity (time required to achieve 99.9% of growth reduction was 2.1 to 3.2 h). The presence of serum enhanced caspofungin activity but lowered those of micafungin and AMB...
  27. pmc Synergistic activities of fluconazole and voriconazole with terbinafine against four Candida species determined by checkerboard, time-kill, and Etest methods
    Emilia Cantón
    Unidad de Microbiologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avenida Campanar 21, 46009 Valencia, Spain
    Antimicrob Agents Chemother 49:1593-6. 2005
    ..Synergism (C. albicans, C. glabrata, and C. tropicalis) and indifference (C. krusei) were observed. Correlation among methods was good. The Etest is a suitable method to determine drug interactions...
  28. pmc Statistical analyses of correlation between fluconazole MICs for Candida spp. assessed by standard methods set forth by the European Committee on Antimicrobial Susceptibility Testing (E.Dis. 7.1) and CLSI (M27-A2)
    Juan Luis Rodriguez-Tudela
    Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Ctra Majadahonda Pozuelo km 2, 28220 Majadahonda, Madrid, Spain
    J Clin Microbiol 45:109-11. 2007
    ..In summary, both methods produce very similar MICs, indicating that methodology does not pose any obstacle to obtaining uniform standards for antifungal susceptibility testing of yeasts...
  29. pmc Comparison of the Sensititre YeastOne colorimetric antifungal panel with a modified NCCLS M38-A method to determine the activity of voriconazole against clinical isolates of Aspergillus spp
    Carmen Castro
    Servicio de Microbiologia, Hospital Universitario de Valme, Seville, Spain
    J Clin Microbiol 42:4358-60. 2004
    ..5% for Aspergillus flavus. Discrepancies between the methods were due to higher Sensititre MICs. The Sensititre YeastOne method could have potential value for susceptibility testing of Aspergillus spp. to voriconazole...
  30. pmc Patterns of amphotericin B killing kinetics against seven Candida species
    Emilia Cantón
    Unidad de Microbiologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avenida Campanar 21, 46009 Valencia, Spain
    Antimicrob Agents Chemother 48:2477-82. 2004
    ..The failure of AMB treatment could be due to its poor killing activity against some species at the concentrations reached in patients' serum...
  31. ncbi request reprint An open-label comparative pilot study of oral voriconazole and itraconazole for long-term treatment of paracoccidioidomycosis
    Flavio Queiroz-Telles
    Department of Community Health, Hospital de Clinicas de Curitiba Universidade Federal do Paraná, Curitiba, Parana, Brazil
    Clin Infect Dis 45:1462-9. 2007
    ..A pilot study was conducted to investigate the efficacy, safety, and tolerability of voriconazole for the long-term treatment of acute or chronic paracoccidioidomycosis, with itraconazole as the control treatment...
  32. ncbi request reprint Comparison of disc diffusion assay with the CLSI reference method (M27-A2) for testing in vitro posaconazole activity against common and uncommon yeasts
    Emilia Cantón
    Unidad de Microbiologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Valencia, Spain
    J Antimicrob Chemother 61:135-8. 2008
    ..To evaluate the suitability of disc diffusion (DD) assay for testing posaconazole activity and to corroborate its activity against recently isolated yeasts by the CLSI reference microdilution M27-A2 method...
  33. ncbi request reprint Clinical research in the lay press: irresponsible journalism raises a huge dose of doubt
    Elias J Anaissie
    Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Clin Infect Dis 43:1031-9. 2006
  34. ncbi request reprint Status of medical mycology education
    William J Steinbach
    Department of Pediatrics, Division of Pediatric Infectious Diseases, Duke University Medical Center, Durham, NC 27710, USA
    Med Mycol 41:457-67. 2003
    ....