Rafael V Davalos

Summary

Affiliation: Virginia Polytechnic Institute and State University
Country: USA

Publications

  1. pmc Sphingolipid metabolites modulate dielectric characteristics of cells in a mouse ovarian cancer progression model
    Alireza Salmanzadeh
    Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Integr Biol (Camb) 5:843-52. 2013
  2. pmc High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction
    Christopher B Arena
    Bioelectromechanical Systems Lab, Virginia Tech Wake Forest University School of Biomedical Engineering and Sciences, 330 ICTAS Building MC0298, Blacksburg, VA 24061, USA
    Biomed Eng Online 10:102. 2011
  3. doi request reprint Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature
    Alireza Salmanzadeh
    Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Lab Chip 12:182-9. 2012
  4. doi request reprint Modeling and development of a low frequency contactless dielectrophoresis (cDEP) platform to sort cancer cells from dilute whole blood samples
    Michael B Sano
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Biosens Bioelectron 30:13-20. 2011
  5. doi request reprint Selective concentration of human cancer cells using contactless dielectrophoresis
    Erin A Henslee
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA, USA
    Electrophoresis 32:2523-9. 2011
  6. pmc A parametric study delineating irreversible electroporation from thermal damage based on a minimally invasive intracranial procedure
    Paulo A Garcia
    Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA, USA
    Biomed Eng Online 10:34. 2011
  7. doi request reprint Theoretical considerations of tissue electroporation with high-frequency bipolar pulses
    Christopher B Arena
    Virginia Tech Wake Forest School of Biomedical Engineering and Sciences, Bioelectromechanical Systems Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    IEEE Trans Biomed Eng 58:1474-82. 2011
  8. doi request reprint Intracranial nonthermal irreversible electroporation: in vivo analysis
    Paulo A Garcia
    Bioelectromechanical Systems BEMS Laboratory, School of Biomedical Engineering and Sciences SBES, Virginia Tech Wake Forest University, Virginia Tech, 329 ICTAS Building, Stanger Street MC 0298, Blacksburg, VA 24061, USA
    J Membr Biol 236:127-36. 2010
  9. doi request reprint A study using irreversible electroporation to treat large, irregular tumors in a canine patient
    Robert E Neal
    Bioelectromechanical Systems Lab in the Virginia Tech Wake Forest School of Biomedical Engineering and Sciences at Virginia Tech, Blacksburg, VA 24060, USA
    Conf Proc IEEE Eng Med Biol Soc 2010:2747-50. 2010
  10. doi request reprint Non-thermal irreversible electroporation for deep intracranial disorders
    Paulo A Garcia
    Virginia Tech Wake Forest University School of Biomedical Engineering and Sciences, Blacksburg, VA 24061, USA
    Conf Proc IEEE Eng Med Biol Soc 2010:2743-6. 2010

Collaborators

Detail Information

Publications25

  1. pmc Sphingolipid metabolites modulate dielectric characteristics of cells in a mouse ovarian cancer progression model
    Alireza Salmanzadeh
    Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Integr Biol (Camb) 5:843-52. 2013
    ..It also demonstrates the potential of cDEP to be used as a new, rapid technique for drug efficacy studies, and for eventually designing more personalized treatment regimens...
  2. pmc High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction
    Christopher B Arena
    Bioelectromechanical Systems Lab, Virginia Tech Wake Forest University School of Biomedical Engineering and Sciences, 330 ICTAS Building MC0298, Blacksburg, VA 24061, USA
    Biomed Eng Online 10:102. 2011
    ..This study shows that by applying high-frequency, bipolar bursts, muscle contractions can be eliminated during IRE without compromising the non-thermal mechanism of cell death...
  3. doi request reprint Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature
    Alireza Salmanzadeh
    Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Lab Chip 12:182-9. 2012
    ..Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis...
  4. doi request reprint Modeling and development of a low frequency contactless dielectrophoresis (cDEP) platform to sort cancer cells from dilute whole blood samples
    Michael B Sano
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Biosens Bioelectron 30:13-20. 2011
    ..This is the first cDEP device designed to operate below 100 kHz resulting in successful manipulation of human leukemia cells, while in the background red blood cells are unaffected...
  5. doi request reprint Selective concentration of human cancer cells using contactless dielectrophoresis
    Erin A Henslee
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA, USA
    Electrophoresis 32:2523-9. 2011
    ....
  6. pmc A parametric study delineating irreversible electroporation from thermal damage based on a minimally invasive intracranial procedure
    Paulo A Garcia
    Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA, USA
    Biomed Eng Online 10:34. 2011
    ....
  7. doi request reprint Theoretical considerations of tissue electroporation with high-frequency bipolar pulses
    Christopher B Arena
    Virginia Tech Wake Forest School of Biomedical Engineering and Sciences, Bioelectromechanical Systems Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    IEEE Trans Biomed Eng 58:1474-82. 2011
    ..Specifically, high-frequency fields ranging from 500 kHz to 1 MHz are best suited to penetrate epithelial layers without inducing significant Joule heating, and cause electroporation in underlying cells...
  8. doi request reprint Intracranial nonthermal irreversible electroporation: in vivo analysis
    Paulo A Garcia
    Bioelectromechanical Systems BEMS Laboratory, School of Biomedical Engineering and Sciences SBES, Virginia Tech Wake Forest University, Virginia Tech, 329 ICTAS Building, Stanger Street MC 0298, Blacksburg, VA 24061, USA
    J Membr Biol 236:127-36. 2010
    ..Knowledge of the dynamic electrical conductivity of the tissue and electric field that correlates to lesion volume is crucial to ensure predictable complete NTIRE treatment while minimizing damage to surrounding healthy tissue...
  9. doi request reprint A study using irreversible electroporation to treat large, irregular tumors in a canine patient
    Robert E Neal
    Bioelectromechanical Systems Lab in the Virginia Tech Wake Forest School of Biomedical Engineering and Sciences at Virginia Tech, Blacksburg, VA 24060, USA
    Conf Proc IEEE Eng Med Biol Soc 2010:2747-50. 2010
    ..The executed plan was able to alleviate the patient's clinical symptoms without damaging any of the nearby sensitive tissues in a complex heterogeneous environment...
  10. doi request reprint Non-thermal irreversible electroporation for deep intracranial disorders
    Paulo A Garcia
    Virginia Tech Wake Forest University School of Biomedical Engineering and Sciences, Blacksburg, VA 24061, USA
    Conf Proc IEEE Eng Med Biol Soc 2010:2743-6. 2010
    ..We also confirm the non-thermal aspect of the technique with real time temperature data measured at the electrode-tissue interface...
  11. pmc Towards the creation of decellularized organ constructs using irreversible electroporation and active mechanical perfusion
    Michael B Sano
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA, USA
    Biomed Eng Online 9:83. 2010
    ..This work outlines a method of organ decellularization using non-thermal irreversible electroporation (N-TIRE) which, in combination with reseeding, may help supplement the supply of organs for transplant...
  12. doi request reprint The feasibility of irreversible electroporation for the treatment of breast cancer and other heterogeneous systems
    Robert E Neal
    Bioelectromechanical Systems, Virginia Tech Wake Forest School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, 329 ICTAS Building, Stranger Street, Blacksburg, VA, 24061, USA
    Ann Biomed Eng 37:2615-25. 2009
    ..Information from this study may be used for the investigation of other heterogeneous tissue applications for IRE...
  13. doi request reprint Contactless dielectrophoretic spectroscopy: examination of the dielectric properties of cells found in blood
    Michael B Sano
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Electrophoresis 32:3164-71. 2011
    ....
  14. doi request reprint Microfluidic mixing using contactless dielectrophoresis
    Alireza Salmanzadeh
    Bioelectromechanical Systems Laboratory, Biomedical Engineering Department, Virginia Tech, Blacksburg, VA, USA
    Electrophoresis 32:2569-78. 2011
    ..This approach shows potential for mixing low diffusivity biological samples, which is a very challenging problem in laminar flows at small scales...
  15. doi request reprint Pilot study of irreversible electroporation for intracranial surgery
    Paulo A Garcia
    Virginia Tech Wake Forest University School of Biomedical Engineering and Sciences, Blacksburg, VA 24061, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:6513-6. 2009
    ..This preliminary study is the first step towards using irreversible electroporation as a brain cancer treatment...
  16. doi request reprint Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)
    Hadi Shafiee
    Engineering Science and Mechanics Department, Virginia Tech, Blacksburg, VA 24061, USA
    Lab Chip 10:438-45. 2010
    ..In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells...
  17. doi request reprint Theoretical study for the treatment of pancreatic cancer using electric pulses
    Christopher B Arena
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24060, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:5997-6000. 2009
    ....
  18. doi request reprint Experimental characterization and numerical modeling of tissue electrical conductivity during pulsed electric fields for irreversible electroporation treatment planning
    Robert E Neal
    Bioelectromechanical Systems Laboratory, Virginia Tech Wake Forest School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA 24060, USA
    IEEE Trans Biomed Eng 59:1076-85. 2012
    ..We conclude that significant changes in predicted outcomes will occur when the experimental results are applied to the numerical model, where the direction and degree of change varies with the electric field considered...
  19. doi request reprint Disabled-2 modulates homotypic and heterotypic platelet interactions by binding to sulfatides
    John D Welsh
    Integrated Cellular Responses Laboratory, Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Br J Haematol 154:122-33. 2011
    ..Moreover, the regulatory role of Dab2 N-PTB extends to platelet-leucocyte adhesion and aggregation events, suggesting a multi-target role for Dab2 in haemostasis...
  20. pmc Treatment of breast cancer through the application of irreversible electroporation using a novel minimally invasive single needle electrode
    Robert E Neal
    Bioelectromechanical Systems Lab, Virginia Tech Wake Forest School of Biomedical Engineering and Sciences, Virginia Tech, 329 ICTAS Building, MC0298, Stranger Street, Blacksburg, VA 24061, USA
    Breast Cancer Res Treat 123:295-301. 2010
    ..Tumor regression was observed in 5 out of 7 MDA-MB231 human mammary tumors orthotopically implanted in female Nu/Nu mice, with continued growth in controls...
  21. doi request reprint Electrical conductivity changes during irreversible electroporation treatment of brain cancer
    Paulo A Garcia
    Virginia Tech Wake Forest University School of Biomedical Engineering and Sciences, Blacksburg, VA 24061, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:739-42. 2011
    ..42-3.67 times the baseline conductivity, confirming the physical phenomenon that has been detected in other tissues undergoing similar electroporation-based treatments...
  22. doi request reprint Experimental characterization of intrapulse tissue conductivity changes for electroporation
    Robert E Neal
    Bioelectromechanical Systems Laboratory, Virginia Tech Wake Forest School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA 24060, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:5581-4. 2011
    ..This conductivity curve was used in a numerical model to demonstrate the impact of accounting for these changes when modeling electric field distributions to develop treatment plans...
  23. doi request reprint A low cost solution for the fabrication of dielectrophoretic microfluidic devices and embedded electrodes
    Michael B Sano
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, Blacksburg, VA 24061, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:8384-7. 2011
    ..These results illustrate this technique's potential to produce microfluidic devices with embedded electrodes for lab on chip applications while significantly reducing fabrication expense...
  24. doi request reprint A preliminary study to delineate irreversible electroporation from thermal damage using the arrhenius equation
    Hadi Shafiee
    Bioelectromechanical Systems Laboratory, Department of Engineering Science and Mechanics, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    J Biomech Eng 131:074509. 2009
    ..These results confirm that the region to induce IRE without thermal damage is substantial. We believe that our new theoretical analysis will allow researchers to optimize IRE parameters without inducing deleterious thermal effects...
  25. doi request reprint Electromagnetically controlled biological assembly of aligned bacterial cellulose nanofibers
    Michael B Sano
    School of Biomedical Engineering and Sciences, Virginia Tech Wake Forest University, 329 ICTAS Building, Stanger Street MC 0298, Blacksburg, VA 24061, USA
    Ann Biomed Eng 38:2475-84. 2010
    ..xylinum in the presence of electric fields in micro- and macro-environments. This study demonstrates a new concept of bottom up material synthesis by the control of a biological assembly process...