William Barton

Summary

Affiliation: Virginia Commonwealth University
Country: USA

Publications

  1. ncbi Highly efficient selenomethionine labeling of recombinant proteins produced in mammalian cells
    William A Barton
    Department of Biochemistry, Virginia Commonwealth University, Richmond, 23298, USA
    Protein Sci 15:2008-13. 2006
  2. ncbi Tie1-Tie2 interactions mediate functional differences between angiopoietin ligands
    Tom C M Seegar
    Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, 1101 East Marshall Street, Richmond, VA 23298, USA
    Mol Cell 37:643-55. 2010
  3. ncbi Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering
    William A Barton
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 99:13397-402. 2002
  4. ncbi Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC
    Jacob Lesniak
    Joan and Sanford I Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Protein Sci 12:2838-43. 2003
  5. ncbi Structure-based enzyme engineering and its impact on in vitro glycorandomization
    Jon S Thorson
    Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, University of Wisconsin - Madison, Madison, WI 53705, USA
    Chembiochem 5:16-25. 2004
  6. ncbi Structure and axon outgrowth inhibitor binding of the Nogo-66 receptor and related proteins
    William A Barton
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    EMBO J 22:3291-302. 2003
  7. ncbi Crystal structure of the ephrin-B1 ectodomain: implications for receptor recognition and signaling
    Dimitar B Nikolov
    Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
    Biochemistry 44:10947-53. 2005
  8. ncbi Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans
    Peter W Janes
    Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, Australia
    Cell 123:291-304. 2005
  9. ncbi Crystal structures of the Tie2 receptor ectodomain and the angiopoietin-2-Tie2 complex
    William A Barton
    Structural Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    Nat Struct Mol Biol 13:524-32. 2006
  10. ncbi Structural and functional characterization of the Pseudomonas hydroperoxide resistance protein Ohr
    Jacob Lesniak
    Joan and Sanford I Weill Graduate School of Medical Sciences of Cornell University, New York City, NY 10021, USA
    EMBO J 21:6649-59. 2002

Research Grants

  1. Biochemical and Biophysical Analysis of Angiopoietin Signaling
    William A Barton; Fiscal Year: 2010

Collaborators

  • Dimitar B Nikolov
  • Jacob Lesniak
  • Tom C M Seegar
  • Momchil V Kolev
  • Peter W Janes
  • Jon S Thorson
  • Scott C Henderson
  • Dorothea Tzvetkova-Robev
  • Becca Eller
  • Juha Pekka Himanen
  • Carl P Blobel
  • Eva Nievergall
  • Juha-Pekka Himanen
  • Nayanendu Saha
  • Martin Lackmann
  • Sabine H Wimmer-Kleikamp
  • Christoph Albermann
  • Dirk Hoffmeister

Detail Information

Publications11

  1. ncbi Highly efficient selenomethionine labeling of recombinant proteins produced in mammalian cells
    William A Barton
    Department of Biochemistry, Virginia Commonwealth University, Richmond, 23298, USA
    Protein Sci 15:2008-13. 2006
    ..We therefore evaluated several variables that play roles in determining incorporation levels and report here a simple protocol for selenomethionine modification of proteins in mammalian cells routinely yielding >90% labeling efficiency...
  2. ncbi Tie1-Tie2 interactions mediate functional differences between angiopoietin ligands
    Tom C M Seegar
    Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, 1101 East Marshall Street, Richmond, VA 23298, USA
    Mol Cell 37:643-55. 2010
    ..We further demonstrate that the Tie1-Tie2 interactions are dynamic, inhibitory, and differentially modulated by angiopoietin-1 and -2. Based on the available data, we propose a unified model for angiopoietin-induced Tie2 signaling...
  3. ncbi Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering
    William A Barton
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 99:13397-402. 2002
    ..In aggregate, our results provide valuable blueprints for altering nucleotidylyltransferase specificity by design, which is the first step toward in vitro glycorandomization...
  4. ncbi Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC
    Jacob Lesniak
    Joan and Sanford I Weill Graduate School of Medical Sciences of Cornell University, New York, New York 10021, USA
    Protein Sci 12:2838-43. 2003
    ....
  5. ncbi Structure-based enzyme engineering and its impact on in vitro glycorandomization
    Jon S Thorson
    Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, University of Wisconsin - Madison, Madison, WI 53705, USA
    Chembiochem 5:16-25. 2004
  6. ncbi Structure and axon outgrowth inhibitor binding of the Nogo-66 receptor and related proteins
    William A Barton
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    EMBO J 22:3291-302. 2003
    ..The NgR structure analysis, as well as a comparison of NgR surface residues not conserved in NgR2 and NgR3, identifies potential protein interaction sites important in the assembly of a functional signaling complex...
  7. ncbi Crystal structure of the ephrin-B1 ectodomain: implications for receptor recognition and signaling
    Dimitar B Nikolov
    Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
    Biochemistry 44:10947-53. 2005
    ..The implications of the ephrin-B1 structure, in context of available ephrin-B1 mutagenesis data, for the mechanism of Eph-ephrin recognition and signaling initiation are discussed...
  8. ncbi Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans
    Peter W Janes
    Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, Australia
    Cell 123:291-304. 2005
    ..Our data suggest a simple mechanism for regulating ADAM10-mediated ephrin proteolysis, which ensures that only Eph bound ephrins are recognized and cleaved...
  9. ncbi Crystal structures of the Tie2 receptor ectodomain and the angiopoietin-2-Tie2 complex
    William A Barton
    Structural Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    Nat Struct Mol Biol 13:524-32. 2006
    ..Analysis of the structures and structure-based mutagenesis provide insight into the mechanism of receptor activation and support the hypothesis that all angiopoietins interact with Tie2 in a structurally similar manner...
  10. ncbi Structural and functional characterization of the Pseudomonas hydroperoxide resistance protein Ohr
    Jacob Lesniak
    Joan and Sanford I Weill Graduate School of Medical Sciences of Cornell University, New York City, NY 10021, USA
    EMBO J 21:6649-59. 2002
    ..We propose that the Ohr catalytic mechanism is similar to that of the structurally unrelated peroxiredoxins, directly utilizing highly reactive cysteine thiol groups to elicit hydroperoxide reduction...
  11. ncbi Structure of the angiopoietin-2 receptor binding domain and identification of surfaces involved in Tie2 recognition
    William A Barton
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    Structure 13:825-32. 2005
    ..The structure reveals a fibrinogen fold with a unique C-terminal P domain. Conservation analysis and structure-based mutagenesis identify a groove on the Ang2 molecular surface that mediates receptor recognition...

Research Grants3

  1. Biochemical and Biophysical Analysis of Angiopoietin Signaling
    William A Barton; Fiscal Year: 2010
    ....