John V Williams

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. pmc Population-based incidence of human metapneumovirus infection among hospitalized children
    John V Williams
    Department of Pediatrics, Vanderbilt University Schoolof Medicine, and Monroe Carell Jr Children s Hospital at Vanderbilt, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 201:1890-8. 2010
  2. pmc Association of human metapneumovirus with acute otitis media
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Int J Pediatr Otorhinolaryngol 70:1189-93. 2006
  3. pmc The role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Infect Dis 193:387-95. 2006
  4. pmc A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo
    John V Williams
    Pediatric Infectious Diseases, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232
    J Virol 81:8315-24. 2007
  5. pmc A prospective study comparing human metapneumovirus with other respiratory viruses in adults with hematologic malignancies and respiratory tract infections
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 192:1061-5. 2005
  6. pmc The cotton rat (Sigmodon hispidus) is a permissive small animal model of human metapneumovirus infection, pathogenesis, and protective immunity
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    J Virol 79:10944-51. 2005
  7. pmc The clinical presentation and outcomes of children infected with newly identified respiratory tract viruses
    John V Williams
    Department of Pediatrics, Division of Pediatric Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, 37232 2581, USA
    Infect Dis Clin North Am 19:569-84. 2005
  8. pmc Viral etiologies of infant bronchiolitis, croup and upper respiratory illness during 4 consecutive years
    E Kathryn Miller
    From the Departments of Pediatrics, Biostatistics, Medicine, and Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, TN
    Pediatr Infect Dis J 32:950-5. 2013
  9. pmc Coronavirus infection and hospitalizations for acute respiratory illness in young children
    H Keipp B Talbot
    Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Med Virol 81:853-6. 2009
  10. pmc Burden of human metapneumovirus infection in young children
    Kathryn M Edwards
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232 2581, USA
    N Engl J Med 368:633-43. 2013

Research Grants

Detail Information

Publications68

  1. pmc Population-based incidence of human metapneumovirus infection among hospitalized children
    John V Williams
    Department of Pediatrics, Vanderbilt University Schoolof Medicine, and Monroe Carell Jr Children s Hospital at Vanderbilt, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 201:1890-8. 2010
    ..Human metapneumovirus (HMPV) is a leading cause of acute respiratory illness (ARI) in children. Population-based incidence rates and comprehensive clinical characterizations of disease have not been established...
  2. pmc Association of human metapneumovirus with acute otitis media
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Int J Pediatr Otorhinolaryngol 70:1189-93. 2006
    ..In the current study, we sought to investigate an association between hMPV and children presenting with AOM as a primary diagnosis...
  3. pmc The role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Infect Dis 193:387-95. 2006
    ..The role that human metapneumovirus (hMPV) plays in the etiology of upper respiratory tract infections (URIs) in children over a period of many years has not been evaluated previously...
  4. pmc A recombinant human monoclonal antibody to human metapneumovirus fusion protein that neutralizes virus in vitro and is effective therapeutically in vivo
    John V Williams
    Pediatric Infectious Diseases, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232
    J Virol 81:8315-24. 2007
    ..There was a dose-response relationship between the dose of DS7 and virus titer. Human Fab DS7 may have prophylactic or therapeutic potential against severe hMPV infection...
  5. pmc A prospective study comparing human metapneumovirus with other respiratory viruses in adults with hematologic malignancies and respiratory tract infections
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 192:1061-5. 2005
    ..Nine patients with hMPV developed LRI; 3 of these patients died. hMPV is a common cause of respiratory infections in adults with hematologic malignancies, with associated morbidity and mortality...
  6. pmc The cotton rat (Sigmodon hispidus) is a permissive small animal model of human metapneumovirus infection, pathogenesis, and protective immunity
    John V Williams
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    J Virol 79:10944-51. 2005
    ..This model will allow further in vivo studies of hMPV pathogenesis and evaluation of vaccine candidates...
  7. pmc The clinical presentation and outcomes of children infected with newly identified respiratory tract viruses
    John V Williams
    Department of Pediatrics, Division of Pediatric Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN, 37232 2581, USA
    Infect Dis Clin North Am 19:569-84. 2005
    ..Several zoonotic paramyxoviruses, including Nipah and Hendra viruses, have emerged as occasional causes of sever outbreaks of respiratory tract illness in children and adults...
  8. pmc Viral etiologies of infant bronchiolitis, croup and upper respiratory illness during 4 consecutive years
    E Kathryn Miller
    From the Departments of Pediatrics, Biostatistics, Medicine, and Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, TN
    Pediatr Infect Dis J 32:950-5. 2013
    ..Prospective data on viral etiology and clinical characteristics of bronchiolitis and upper respiratory illness (URI) in infants are limited...
  9. pmc Coronavirus infection and hospitalizations for acute respiratory illness in young children
    H Keipp B Talbot
    Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Med Virol 81:853-6. 2009
    ..2 (95% CI 4.3, 17.6), 4.2 (95% CI 1.9, 6.9), and 0 (95% CI 0, 3.7) in children aged <6 months, 6-23 months, and 24-59 months, respectively. Coronaviruses were identified in a modest number of hospitalized children...
  10. pmc Burden of human metapneumovirus infection in young children
    Kathryn M Edwards
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232 2581, USA
    N Engl J Med 368:633-43. 2013
    ..The inpatient and outpatient burden of human metapneumovirus (HMPV) infection among young children has not been well established...
  11. pmc The pediatric burden of human coronaviruses evaluated for twenty years
    H Keipp Talbot
    Division of Infectious Diseases, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA
    Pediatr Infect Dis J 28:682-7. 2009
    ..The epidemiology of human coronaviruses (HCoVs) has not been established using reverse transcription polymerase chain reaction techniques in a specimen collection that spans decades...
  12. pmc Human rhinovirus C: Age, season, and lower respiratory illness over the past 3 decades
    Jodell E Linder
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Allergy Clin Immunol 131:69-77.e1-6. 2013
    ..Human rhinoviruses (HRVs) cause common colds, and the recently discovered HRV-C is increasingly associated with lower respiratory illness among populations such as children and asthmatic patients...
  13. pmc Influence of maternal asthma on the cause and severity of infant acute respiratory tract infections
    Kecia N Carroll
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA
    J Allergy Clin Immunol 129:1236-42. 2012
    ..Respiratory syncytial virus (RSV) and rhinovirus infections are the most common significant infant respiratory tract illnesses and are associated with increased but differential risks of childhood asthma...
  14. pmc Effectiveness of seasonal vaccine in preventing confirmed influenza-associated hospitalizations in community dwelling older adults
    H Keipp Talbot
    Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Infect Dis 203:500-8. 2011
    ..Current evidence supporting the effectiveness of influenza vaccine in preventing hospitalizations in older adults is insufficient...
  15. doi request reprint A novel group of rhinoviruses is associated with asthma hospitalizations
    E Kathryn Miller
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 9500, USA
    J Allergy Clin Immunol 123:98-104.e1. 2009
    ..Although recent studies have identified new group C human rhinoviruses (HRVCs), their spectrum of pediatric disease is unknown...
  16. pmc Human metapneumovirus fusion protein vaccines that are immunogenic and protective in cotton rats
    Gabriella Cseke
    Department of Chemistry, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Ave South, Nashville, TN 37232 2581, USA
    J Virol 81:698-707. 2007
    ..We conclude that sequence-optimized hMPV F protein protects against hMPV infection when delivered as either a DNA or a protein vaccine in cotton rats...
  17. pmc Human metapneumovirus in hospitalized children in Amman, Jordan
    Syed Asad Ali
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    J Med Virol 82:1012-6. 2010
    ..Longer surveillance studies are needed to better understand the seasonal epidemiology of HMPV and to assess if co-infection with HMPV and RSV leads to more severe illness...
  18. pmc Real-world comparison of two molecular methods for detection of respiratory viruses
    S Asad Ali
    Department of Pediatrics, Vanderbilt University School of Medicine, 1161 21st Avenue South, Nashville, TN 37232, USA
    Virol J 8:332. 2011
    ..We sought to perform a real-world comparison of two molecular respiratory viral diagnostic methods between two experienced respiratory virus research laboratories...
  19. pmc Differential pathogenesis of respiratory syncytial virus clinical isolates in BALB/c mice
    Kate L Stokes
    Department of Pediatrics, Emory University, Atlanta, Georgia 30322, USA
    J Virol 85:5782-93. 2011
    ..We found that RSV clinical isolates induce variable pathogenesis in mice, and we established a mouse model of clinical isolate strain-dependent RSV pathogenesis that recapitulates key features of RSV disease...
  20. pmc Human metapneumovirus virus-like particles induce protective B and T cell responses in a mouse model
    Reagan G Cox
    Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    J Virol 88:6368-79. 2014
    ..These results suggest that nonreplicating VLPs are a promising vaccine candidate for HMPV...
  21. doi request reprint Respiratory syncytial virus-associated hospitalizations among children less than 24 months of age
    Caroline Breese Hall
    Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Pediatrics 132:e341-8. 2013
    ..However, estimates of the RSV hospitalization burden have varied, and precision has been limited by the use of age strata grouped in blocks of 6 to ≥ 12 months...
  22. pmc Respiratory virus shedding in a cohort of on-duty healthcare workers undergoing prospective surveillance
    Jennifer C Esbenshade
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Infect Control Hosp Epidemiol 34:373-8. 2013
    ..Healthcare-associated transmission of respiratory viruses is a concerning patient safety issue...
  23. pmc Rates of hospitalizations for respiratory syncytial virus, human metapneumovirus, and influenza virus in older adults
    Kyle Widmer
    Department of Medicine, VA TN Valley Health Care System, Nashville, Tennessee 37205, USA
    J Infect Dis 206:56-62. 2012
    ..We performed a prospective study to determine the disease burden of respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) in older adults in comparison with influenza virus...
  24. pmc An alphavirus replicon-based human metapneumovirus vaccine is immunogenic and protective in mice and cotton rats
    Hoyin Mok
    Departments of Pediatrics, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Virol 82:11410-8. 2008
    ..Aberrant cytokine gene expression was not detected in these animals. Together, these results represent an important first step toward the use of VRPs encoding hMPV F proteins as a prophylactic vaccine for hMPV...
  25. pmc Soluble recombinant human metapneumovirus G protein is immunogenic but not protective
    Alex B Ryder
    Vanderbilt University Medical Center, School of Medicine, Nashville, TN 37232 2581, USA
    Vaccine 28:4145-52. 2010
    ..Unlike the analogous non-fusion glycoproteins of other human paramyxoviruses, HMPV G does not appear to be a protective antigen. This represents an unusual feature of HMPV...
  26. doi request reprint Diagnostic performance of the BinaxNow Influenza A&B rapid antigen test in ED patients
    Wesley H Self
    Department of Emergency Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 4700, USA
    Am J Emerg Med 30:1955-61. 2012
    ..The objective of this study is to evaluate the diagnostic performance of the BinaxNow Influenza A&B rapid antigen test (RAT) in emergency department (ED) patients...
  27. pmc Emergency department visits for influenza A(H1N1)pdm09, Davidson County, Tennessee, USA
    Wesley H Self
    Vanderbilt University, Nashville, Tennessee 37212, USA
    Emerg Infect Dis 18:863-5. 2012
    ..We estimated ≈10 visits per 1,000 residents during the pandemic period. This estimate should help emergency departments prepare for future pandemics...
  28. pmc Viral acute lower respiratory infections impair CD8+ T cells through PD-1
    John J Erickson
    Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA
    J Clin Invest 122:2967-82. 2012
    ..Therefore, the PD-1/PD-L1 pathway may represent a therapeutic target in the treatment of respiratory viruses...
  29. pmc Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years
    Chin Fen Yang
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA
    Virol J 6:138. 2009
    ..Human metapneumovirus (HMPV) is an important cause of acute respiratory illness in children. We examined the diversity and molecular evolution of HMPV using 85 full-length F (fusion) gene sequences collected over a 20-year period...
  30. doi request reprint Effectiveness of influenza vaccine for preventing laboratory-confirmed influenza hospitalizations in adults, 2011-2012 influenza season
    H Keipp Talbot
    Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Clin Infect Dis 56:1774-7. 2013
    ..Influenza vaccination was verified in 35% (6/17) of adults with influenza-associated hospitalizations compared to 64% (97/152) of test-negative controls; adjusted vaccine effectiveness was 71.4% (95% confidence interval, 17.1%-94.9%)...
  31. pmc Integrin alphavbeta1 promotes infection by human metapneumovirus
    Gabriella Cseke
    Department of Chemistry, Vanderbilt University College of Arts and Sciences, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 106:1566-71. 2009
    ..Recombinant hMPV F protein binds to cells, whereas Arg-Gly-Glu (RGE)-mutant F protein does not. These data suggest that alphavbeta1 integrin is a functional receptor for hMPV...
  32. pmc Adherence to guidelines for respiratory syncytial virus immunoprophylaxis among infants with prematurity or chronic lung disease in three United States counties
    Kecia N Carroll
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Pediatr Infect Dis J 31:e229-31. 2012
    ..Immunoprophylaxis among infants who met national criteria for prophylaxis increased from 33% to 83% over the 6-year period; 17% (11/65) of infants who received immunoprophylaxis did not meet eligibility criteria...
  33. pmc Real-time reverse transcriptase PCR assay for improved detection of human metapneumovirus
    Jennifer Klemenc
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, United States
    J Clin Virol 54:371-5. 2012
    ..Human metapneumovirus (HMPV) is a paramyxovirus with multiple genetic lineages that is a leading cause of acute respiratory disease. Several RT-PCR assays have been described based on limited available sequence data...
  34. pmc A mechanistic role for type III IFN-λ1 in asthma exacerbations mediated by human rhinoviruses
    E Kathryn Miller
    Department of Pediatrics, Vanderbilt University, Nashville, TN 37232, USA
    Am J Respir Crit Care Med 185:508-16. 2012
    ..Moreover, although several hypotheses have been advanced to explain virus-induced exacerbations, their mechanism remains unclear...
  35. pmc The Tennessee Children's Respiratory Initiative: Objectives, design and recruitment results of a prospective cohort study investigating infant viral respiratory illness and the development of asthma and allergic diseases
    Tina V Hartert
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 8300, USA
    Respirology 15:691-9. 2010
    ..The primary goals are to investigate both the acute and the long-term health consequences of varying severity and aetiology of clinically significant viral respiratory tract infections on early childhood outcomes...
  36. pmc Influenza in older adults: impact of vaccination of school children
    H Keipp Talbot
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States
    Vaccine 27:1923-7. 2009
    ..59, 2.04) in the intervention county and 1.53 (95% CI 0.71, 2.34) in the control county. These rates did not differ significantly except in the subgroup aged 50 -- 64 years where rates in the intervention county were significantly lower...
  37. pmc Host and viral factors associated with severity of human rhinovirus-associated infant respiratory tract illness
    E Kathryn Miller
    Department of Pediatrics, Vanderbilt University, Nashville, TN 37232 8300, USA
    J Allergy Clin Immunol 127:883-91. 2011
    ..Risk factors for severe human rhinovirus (HRV)-associated infant illness are unknown...
  38. pmc Failure of routine diagnostic methods to detect influenza in hospitalized older adults
    H Keipp Talbot
    Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Infect Control Hosp Epidemiol 31:683-8. 2010
    ..To define the utility of using routine diagnostic methods to detect influenza in older, hospitalized adults...
  39. pmc Cohort profile: the study of respiratory pathogens in andean children
    Carlos G Grijalva
    Departments of Preventive Medicine, Vanderbilt University School of Medicine, Nashville TN, USA, Pediatrics Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville TN, USA, Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville TN, USA, Instituto de Investigacion Nutricional, Lima, Peru, Swiss Tropical and Public Health Institute, Basel, Switzerland and Hubert Department of Global Health, Rollins School of Public Health, Emory University, Atlanta GA, USA
    Int J Epidemiol 43:1021-30. 2014
    ..Investigators interested in more details about this study or in accessing these resources should contact Dr. Carlos G. Grijalva at Vanderbilt University (carlos.grijalva@vanderbilt.edu). ..
  40. pmc The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins
    Reagan G Cox
    Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Virol 86:12148-60. 2012
    ..These results suggest a stepwise mechanism of HMPV entry mediated by the F protein through its interactions with cellular receptors, including RGD-binding integrins...
  41. pmc The human neonatal B cell response to respiratory syncytial virus uses a biased antibody variable gene repertoire that lacks somatic mutations
    John V Williams
    Department of Pediatrics, Nashville, TN 37232, United States
    Mol Immunol 47:407-14. 2009
    ..These biased features of the RSV-specific repertoire likely contribute to the poor functional Ab response in very young infants...
  42. pmc Estimating age-specific influenza-related hospitalization rates during the pandemic (H1N1) 2009 in Davidson Co, TN
    Astride Jules
    Department of Preventive Medicine, Vanderbilt University School of Medicine, Nashville, TN 37212, USA
    Influenza Other Respir Viruses 6:e63-71. 2012
    ..In April 2009, a pandemic caused by a novel influenza strain, the A(H1N1)pdm09 virus, started. Few age-specific estimates of hospitalizations associated with the first year of circulation of the pandemic virus are available...
  43. pmc Human rhinovirus C associated with wheezing in hospitalised children in the Middle East
    E Kathryn Miller
    Department of Pediatrics, Vanderbilt University, Nashville, TN, USA
    J Clin Virol 46:85-9. 2009
    ..Few studies have investigated the disease burden and genetic diversity of human rhinoviruses (HRVs) in developing countries...
  44. pmc Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children
    John V Williams
    Division of Infectious Diseases, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    N Engl J Med 350:443-50. 2004
    ..We sought to determine the role of human metapneumovirus in lower respiratory tract illness in previously healthy infants and children...
  45. pmc Human metapneumovirus nucleoprotein and phosphoprotein interact and provide the minimal requirements for inclusion body formation
    Aaron Derdowski
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Gen Virol 89:2698-708. 2008
    ..This work showed that HMPV N and P proteins provide the minimal viral requirements for HMPV inclusion body formation, which may be a distinguishing characteristic of members of the subfamily Pneumovirinae...
  46. pmc Concordance between RT-PCR-based detection of respiratory viruses from nasal swabs collected for viral testing and nasopharyngeal swabs collected for bacterial testing
    Carlos G Grijalva
    Department of Health Policy, Vanderbilt University School of Medicine, Nashville, TN, United States Electronic address
    J Clin Virol 60:309-12. 2014
    ..Epidemiologic studies of respiratory infections frequently rely on separate sample collections for the detection of bacteria and viruses. The requirement for two specimens presents cost, logistical, and acceptability challenges...
  47. pmc Human metapneumovirus infection plays an etiologic role in acute asthma exacerbations requiring hospitalization in adults
    John V Williams
    Departments of Pediatric, Vanderbilt University Medical Center, Nashville, TN 37232 8300, USA
    J Infect Dis 192:1149-53. 2005
    ..9-312]; P=.03). None of the patients with hMPV infection at hospitalization tested positive at follow-up, strongly suggesting that hMPV plays a direct etiologic role in acute asthma exacerbations...
  48. pmc Effectiveness of non-adjuvanted pandemic influenza A vaccines for preventing pandemic influenza acute respiratory illness visits in 4 U.S. communities
    Marie R Griffin
    Department of Preventive Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 6:e23085. 2011
    ..Inactivated non-adjuvanted pandemic vaccines offered significant protection against confirmed pandemic influenza-associated medical care visits in young adults...
  49. pmc Discovering naturally processed antigenic determinants that confer protective T cell immunity
    Pavlo Gilchuk
    Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Invest 123:1976-87. 2013
    ....
  50. pmc Development of a low-resource RNA extraction cassette based on surface tension valves
    Hali Bordelon
    Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee 37235, USA
    ACS Appl Mater Interfaces 3:2161-8. 2011
    ..5%) to a laboratory-based commercially available kit. These results indicate that this extraction cassette design has the potential to be an effective sample preparation device suitable for use in a low-resource setting...
  51. ncbi request reprint Prevalence of reovirus-specific antibodies in young children in Nashville, Tennessee
    Jennifer H Tai
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2581, USA
    J Infect Dis 191:1221-4. 2005
    ..We observed an age-dependent increase in reovirus-specific antibodies in children 1 year of age and older, peaking at 50.0% in children 5-6 years of age. These findings suggest that reovirus infections are common during early childhood...
  52. pmc Examination of a fusogenic hexameric core from human metapneumovirus and identification of a potent synthetic peptide inhibitor from the heptad repeat 1 region
    Scott A Miller
    Vanderbilt University, Department of Chemistry, Station B 351822, Nashville, TN 37235 1822, USA
    J Virol 81:141-9. 2007
    ..Our studies provide evidence that hMPV also uses a coiled-coil structure as a major player in the fusion process. Additionally, viral HR-1 peptide sequences may need further investigation as potent fusion inhibitors...
  53. pmc A Household-based Study of Acute Viral Respiratory Illnesses in Andean Children
    Philip J Budge
    From the Division of Infectious Diseases, Department of Internal Medicine Department of Preventive Medicine Vanderbilt Vaccine Research Program, Division of Infectious Diseases, Department of Pediatrics Department of Pediatrics Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN Instituto de Investigación Nutricional, Lima, Peru Swiss Tropical and Public Health Institute, Basel, Switzerland and Department of Biostatistics, Vanderbilt University, Nashville, TN
    Pediatr Infect Dis J 33:443-7. 2014
    ..Few community studies have measured the incidence, severity and etiology of acute respiratory illness (ARI) among children living at high-altitude in remote rural settings...
  54. pmc Genomic analysis of four human metapneumovirus prototypes
    Rohith Piyaratna
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232 2581, USA
    Virus Res 160:200-5. 2011
    ..These findings highlight broadly conserved regions of the HMPV genome and suggest unidentified biological roles for SH and G...
  55. ncbi request reprint Immunology of viral respiratory tract infection in infancy
    James E Crowe
    Department of Pediatrics, Vanderbilt University Medical Center, D 7235 Medical Center North, 1161 21st Avenue South, Nashville, TN 37232 2581, USA
    Paediatr Respir Rev 4:112-9. 2003
    ....
  56. pmc Breaking in: human metapneumovirus fusion and entry
    Reagan G Cox
    Vanderbilt University School of Medicine, Department of Pathology, Microbiology and Immunology, Nashville, TN 37232, USA
    Viruses 5:192-210. 2013
    ..Finally, we illuminate key unanswered questions and suggest how further studies can elucidate how this clinically important paramyxovirus fusion protein may have evolved to initiate infection by a unique mechanism...
  57. pmc Studies of culture conditions and environmental stability of human metapneumovirus
    Sharon J Tollefson
    Vanderbilt University Medical Center, School of Medicine, Department of Pediatrics, Nashville, TN, USA
    Virus Res 151:54-9. 2010
    ..These findings provide guidance for laboratories attempting to culture HMPV and relevant information for infection control policies...
  58. ncbi request reprint Medical and economic impact of a respiratory syncytial virus outbreak in a neonatal intensive care unit
    Natasha B Halasa
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232 2581, USA
    Pediatr Infect Dis J 24:1040-4. 2005
    ..We investigated an outbreak of RSV infection in a NICU and its impact on health care delivery, outcomes and costs...
  59. pmc A chimeric A2 strain of respiratory syncytial virus (RSV) with the fusion protein of RSV strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction
    Martin L Moore
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Virol 83:4185-94. 2009
    ..We identified the F protein of RSV line 19 as a factor that plays a role in pulmonary mucin expression in the setting of RSV infection...
  60. doi request reprint Use of intravenous zanamivir after development of oseltamivir resistance in a critically Ill immunosuppressed child infected with 2009 pandemic influenza A (H1N1) virus
    Daniel E Dulek
    Departments of Pediatrics, Vanderbilt University School of Medicine and the Monroe Carell Jr Children s Hospital at Vanderbilt, Nashville, Tennessee, USA
    Clin Infect Dis 50:1493-6. 2010
    ..The regimen was well tolerated and was associated with a decrease in viral burden...
  61. pmc Human coronavirus NL63 is not detected in the respiratory tracts of children with acute Kawasaki disease
    Chisato Shimizu
    Department of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, 92093, USA
    J Infect Dis 192:1767-71. 2005
    ..Only 1 (2%) of 48 patients with acute KD was positive by RT-PCR for HCoV-NL63/NH in a nasopharyngeal swab. These data do not support an association between these HCoVs and KD...
  62. ncbi request reprint Chronic granulomatous disease masquerading as Behçet disease: a case report and review of the literature
    Isaac Thomsen
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Pediatr Infect Dis J 31:529-31. 2012
    ....
  63. pmc Detection of viruses in human adenoid tissues by use of multiplex PCR
    Masatoki Sato
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 47:771-3. 2009
    ..Our results suggest that persistent/latent viral infection in the respiratory tract confounds interpretation of the association of pathogen detection by PCR with acute respiratory infection in these sources...
  64. ncbi request reprint Infant and adult human B cell responses to rotavirus share common immunodominant variable gene repertoires
    Jörn Hendrik Weitkamp
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232 2581, USA
    J Immunol 171:4680-8. 2003
    ..These data suggest that residual fetal bias of the B cell repertoire is not a limiting determinant of the quality of Ab responses to viruses of infants beyond the neonatal period...
  65. pmc Prospective study of the incidence, clinical features, and outcome of symptomatic upper and lower respiratory tract infections by respiratory viruses in adult recipients of hematopoietic stem cell transplants for hematologic malignancies
    Rodrigo Martino
    Department of Clinical Hematology, Hospital de la Santa Creu i Sant Pau, and Autonomous University of Barcelona, Spain
    Biol Blood Marrow Transplant 11:781-96. 2005
    ..In addition, human metapneumovirus should be added to the potentially serious causes of RV infections in HSCT...
  66. pmc Human metapneumovirus infection in children hospitalized for wheezing
    John V Williams
    J Allergy Clin Immunol 115:1311-2. 2005
  67. pmc Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses
    Xiaoyan Lu
    Gastroenteritis and Respiratory Virus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 46:533-9. 2008
    ..The assay was successfully applied in an investigation of a coincidental outbreak of HRV respiratory illness among laboratory staff...
  68. pmc Comparison of the MChip to viral culture, reverse transcription-PCR, and the QuickVue influenza A+B test for rapid diagnosis of influenza
    Martin Mehlmann
    Department of Chemistry and Biochemistry, UCB 215, University of Colorado at Boulder, Boulder, CO 80309, USA
    J Clin Microbiol 45:1234-7. 2007
    ....

Research Grants4

  1. Human Metapneumovirus Infections in Children
    John Williams; Fiscal Year: 2004
    ..The specific aims of this study are to define the prevalence of hMPV infection in children, describe fully the spectrum of clinical illness and identify serologic markers of seroconversion and correlates of immunity. ..
  2. Determinanats of Protective Immunity to Metapneumovirus
    John Williams; Fiscal Year: 2005
    ..The proposed work will provide the candidate skills and experience to be an outstanding physician-scientist in the field of human viral immunology. ..
  3. Cell Entry of Human Metapneumovirus
    John Williams; Fiscal Year: 2008
    ..These studies will increase understanding of how hMPV causes disease in humans, and may help develop targets for antiviral medicines and vaccines. ..
  4. Host determinants of human metapneumovirus immunity and pathogenesis
    John V Williams; Fiscal Year: 2010
    ..Further, the results of this work will increase our understanding of how interactions between respiratory viruses and the immune system contribute to disease and protection. ..