Yi Wei Tang

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. doi request reprint Laboratory diagnosis of CNS infections by molecular amplification techniques
    Yi Wei Tang
    Vanderbilt University Medical Center, 4605 TVC, Nashville, TN 37232 5310, USA 1 615 322 2035 1 615 343 8420
    Expert Opin Med Diagn 1:489-509. 2007
  2. pmc Comparison of protein A gene sequencing with pulsed-field gel electrophoresis and epidemiologic data for molecular typing of methicillin-resistant Staphylococcus aureus
    Y W Tang
    Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 38:1347-51. 2000
  3. pmc Qualitative and quantitative detection of Chlamydophila pneumoniae DNA in cerebrospinal fluid from multiple sclerosis patients and controls
    Yi Wei Tang
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America
    PLoS ONE 4:e5200. 2009
  4. pmc Herpesvirus DNA is consistently detected in lungs of patients with idiopathic pulmonary fibrosis
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 41:2633-40. 2003
  5. ncbi request reprint Molecular diagnostics of atypical pneumonia
    Yi Wei Tang
    Department of Medicine, Molecular Infectious Disease Laboratory, Vanderbilt University Medical Center, TN 37232, USA
    Acta Pharmacol Sin 24:1308-13. 2003
  6. doi request reprint Staphylococcus pseudolugdunensis sp. nov., a pyrrolidonyl arylamidase/ornithine decarboxylase-positive bacterium isolated from blood cultures
    Yi Wei Tang
    Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Diagn Microbiol Infect Dis 60:351-9. 2008
  7. ncbi request reprint Cytokine pattern is solely influenced by priming vaccine but immunity and disease by both priming and boosting vaccines in mice challenged with respiratory syncytial virus
    Yi Wei Tang
    Departments of Medicine and Pathology, Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Virus Res 99:81-7. 2004
  8. ncbi request reprint Detection of hepatitis C virus by a user-developed reverse transcriptase-PCR and use of amplification products for subsequent genotyping
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Clin Virol 31:148-52. 2004
  9. ncbi request reprint Host single-nucleotide polymorphisms and altered responses to inactivated influenza vaccine
    Yi Wei Tang
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Infect Dis 196:1021-5. 2007
  10. ncbi request reprint Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Clin Virol 40:129-34. 2007

Detail Information

Publications90

  1. doi request reprint Laboratory diagnosis of CNS infections by molecular amplification techniques
    Yi Wei Tang
    Vanderbilt University Medical Center, 4605 TVC, Nashville, TN 37232 5310, USA 1 615 322 2035 1 615 343 8420
    Expert Opin Med Diagn 1:489-509. 2007
    ..Although molecular techniques are predicted to be widely used in diagnosing and monitoring CNS infections, the limitations as well as strengths of these techniques must be clearly understood by both clinicians and laboratory personnel...
  2. pmc Comparison of protein A gene sequencing with pulsed-field gel electrophoresis and epidemiologic data for molecular typing of methicillin-resistant Staphylococcus aureus
    Y W Tang
    Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 38:1347-51. 2000
    ....
  3. pmc Qualitative and quantitative detection of Chlamydophila pneumoniae DNA in cerebrospinal fluid from multiple sclerosis patients and controls
    Yi Wei Tang
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America
    PLoS ONE 4:e5200. 2009
    ..pneumoniae DNA in CSF. The TaqMan system may not be sensitive enough for diagnostic purposes due to the low C. pneumoniae copies existing in the majority of CSF specimens from MS patients...
  4. pmc Herpesvirus DNA is consistently detected in lungs of patients with idiopathic pulmonary fibrosis
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 41:2633-40. 2003
    ..05). Immunohistochemistry for EBV or HHV-8 antigen showed viral antigen primarily in airway epithelial cells. These data support the concept that a herpesvirus could be a source of chronic antigenic stimulation in IPF...
  5. ncbi request reprint Molecular diagnostics of atypical pneumonia
    Yi Wei Tang
    Department of Medicine, Molecular Infectious Disease Laboratory, Vanderbilt University Medical Center, TN 37232, USA
    Acta Pharmacol Sin 24:1308-13. 2003
    ..Gradually becoming standardized and widely available, the future will see some promising molecular methods become a mainstay in clinical laboratories for recognition and diagnosis of atypical pneumonia pathogens...
  6. doi request reprint Staphylococcus pseudolugdunensis sp. nov., a pyrrolidonyl arylamidase/ornithine decarboxylase-positive bacterium isolated from blood cultures
    Yi Wei Tang
    Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Diagn Microbiol Infect Dis 60:351-9. 2008
    ..On the basis of the close biochemical reactions with S. lugdunensis and phylogenetic evidence, these isolates are proposed the designation Staphylococcus pseudolugdunensis sp. nov...
  7. ncbi request reprint Cytokine pattern is solely influenced by priming vaccine but immunity and disease by both priming and boosting vaccines in mice challenged with respiratory syncytial virus
    Yi Wei Tang
    Departments of Medicine and Pathology, Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Virus Res 99:81-7. 2004
    ..T helper (Th)-like immune responses were determined solely by the initial vaccination, while weight loss, viral replication, neutralizing antibody responses, and CTL activities were also influenced by boosted vaccinations...
  8. ncbi request reprint Detection of hepatitis C virus by a user-developed reverse transcriptase-PCR and use of amplification products for subsequent genotyping
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Clin Virol 31:148-52. 2004
    ....
  9. ncbi request reprint Host single-nucleotide polymorphisms and altered responses to inactivated influenza vaccine
    Yi Wei Tang
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Infect Dis 196:1021-5. 2007
    ..041). These data indicate that host polymorphisms play a role in determining responses to influenza vaccine...
  10. ncbi request reprint Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Clin Virol 40:129-34. 2007
    ..The Invader Plus method provides another reliable tool for qualitative detection of VZV and differentiation of wild-type and vaccine virus...
  11. pmc StaphPlex system for rapid and simultaneous identification of antibiotic resistance determinants and Panton-Valentine leukocidin detection of staphylococci from positive blood cultures
    Yi Wei Tang
    Department of Medicine, Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232 5310, USA
    J Clin Microbiol 45:1867-73. 2007
    ....
  12. pmc Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 5310, USA
    J Clin Microbiol 43:4830-3. 2005
    ..The miniMAG yielded the highest quantity of nucleic acids, suggesting that this system would be the best for specimens containing a low number of microorganisms of interest...
  13. pmc Duplex PCR assay simultaneously detecting and differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in surgical heart valve specimens
    Yi Wei Tang
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Clin Microbiol 47:2647-50. 2009
    ..quintana, B. henselae, and C. burnetii, respectively, by the dPCR assay, which matched the results obtained by universal bacterial 16S rRNA gene amplification and sequencing...
  14. ncbi request reprint Urine polymerase chain reaction is not as sensitive as urine antigen for the diagnosis of disseminated histoplasmosis
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Diagn Microbiol Infect Dis 54:283-7. 2006
    ..Thus, positive PCR results in urine specimens correlate with positive culture results, but not with antigenuria. The low sensitivity of this PCR assay in urine limits its use in the diagnosis of disseminated histoplasmosis...
  15. pmc Identification of coryneform bacterial isolates by ribosomal DNA sequence analysis
    Y W Tang
    Departments of Medicine and Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 38:1676-8. 2000
    ..5 to 18.5 h. These data demonstrate that the MicroSeq 500 16S bacterial sequencing kit provides a potentially powerful method for the definitive identification of clinical coryneform bacterium isolates...
  16. pmc Comparison of human immunodeficiency virus type 1 RNA sequence heterogeneity in cerebrospinal fluid and plasma
    Y W Tang
    Departments of Medicine, Nashville, Tennessee 37232, and Applied Sciences, Inc, Norcross, Georgia 30092, USA
    J Clin Microbiol 38:4637-9. 2000
    ..Similarly, viral sequences in plasma did not vary over this interval. This study provides evidence that HIV-1 RNA in CSF and plasma arise from distinct compartments...
  17. pmc Characterization of three Staphylococcus aureus isolates from a 17-year-old female who died of tampon-related toxic shock syndrome
    Yi Wei Tang
    Department of Pathology, Vanderbilt University Medical Center, and Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232 5310, USA
    J Clin Microbiol 48:1974-7. 2010
    ....
  18. doi request reprint Staphylococcus aureus: An old pathogen with new weapons
    Yi Wei Tang
    Department of Pathology and Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Clin Lab Med 30:179-208. 2010
    ..This article addresses the emerging aspects of infections caused by S aureus, including microbiology, epidemiology, clinical presentation, pathogenesis, diagnosis, treatment and prognosis, and immunity...
  19. pmc Rapidly increasing prevalence of penicillin-resistant Streptococcus pneumoniae in middle Tennessee: a 10-year clinical and molecular analysis
    Yi Wei Tang
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 40:395-9. 2002
    ..Penicillin resistance is rapidly increasing among S. pneumoniae isolates in Tennessee. The simultaneous detection of S. pneumoniae and high-level penicillin resistance can be accurately performed with the six-primer PCR assay...
  20. pmc Antimicrobial susceptibility patterns and staphylococcal cassette chromosome mec types of, as well as Panton-Valentine leukocidin occurrence among, methicillin-resistant Staphylococcus aureus isolates from children and adults in middle Tennessee
    Abdullah Kilic
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232 5310, USA
    J Clin Microbiol 44:4436-40. 2006
    ..These data indicate that SCCmec-IV MRSA isolates frequently infect children in middle Tennessee and are likely to harbor the PVL gene...
  21. ncbi request reprint QIAamp MinElute virus kit effectively extracts viral nucleic acids from cerebrospinal fluids and nasopharyngeal swabs
    Susan E Sefers
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Clin Virol 35:141-6. 2006
    ..Bothell, WA) for DNA extraction and RNAzol B (Leedo Laboratories Inc., Houston, TX) for RNA extraction, of viral nucleic acids...
  22. pmc Identification and differentiation of clinically relevant mycobacterium species directly from acid-fast bacillus-positive culture broth
    Haijing Li
    Departments of Pathology1 and Medicine, 2 Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Clin Microbiol 47:3814-20. 2009
    ..intracellulare complex strains into M. avium and M. intracellulare may provide a tool to better understand the role of Mycobacterium avium-M. intracellulare complex isolates in human disease...
  23. pmc Identification of Staphylococcus aureus and determination of methicillin resistance directly from positive blood cultures by isothermal amplification and a disposable detection device
    James Goldmeyer
    Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232 5310, USA
    J Clin Microbiol 46:1534-6. 2008
    ..The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively...
  24. ncbi request reprint Prevalence of methicillin-resistant Staphylococcus aureus nasal carriage in the community pediatric population
    Mari M Nakamura
    Department of Pediatrics, Division of Pediatric Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN, USA
    Pediatr Infect Dis J 21:917-22. 2002
    ..Little is known about MRSA nasal carriage rates among children in Nashville, TN and the associated likelihood of community MRSA transmission...
  25. pmc Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients
    Haijing Li
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 41:187-91. 2003
    ....
  26. pmc Clonal dissemination of Staphylococcus epidermidis in an oncology ward
    Kenneth L Muldrew
    Department of Pathology, Vanderbilt University, School of Medicine, Nashville, Tennessee, USA
    J Clin Microbiol 46:3391-6. 2008
    ..This study demonstrates the presence of a predominant clone among central line isolates from an OW that is not present in CoNS venipuncture isolates from an ER...
  27. pmc Major outbreak of toxic shock-like syndrome caused by Streptococcus mitis
    Hong Zhou Lu
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 41:3051-5. 2003
    ..This TSLS outbreak in southeastern China was caused by a toxigenic clone of S. mitis. An apparently novel toxin may explain the unusual virulence of this organism...
  28. pmc Clinical accuracy of a PLEX-ID flu device for simultaneous detection and identification of influenza viruses A and B
    Yi Wei Tang
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Clin Microbiol 51:40-5. 2013
    ....
  29. pmc C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens
    Criziel D Quinn
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 48:603-5. 2010
    ..Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested...
  30. ncbi request reprint Bedside diagnosis of influenzavirus infections in hospitalized children
    Katherine A Poehling
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Pediatrics 110:83-8. 2002
    ..We evaluated the performance of a lateral-flow immunoassay (QuickVue Influenza Test) completed at the bedside of hospitalized children during the influenza season...
  31. pmc Simultaneous amplification and identification of 25 human papillomavirus types with Templex technology
    Jian Han
    Genaco Biomedical Products, Inc, Huntsville, Alabama 35805, USA
    J Clin Microbiol 44:4157-62. 2006
    ..The entire Templex procedure, including DNA extraction, can be completed within 5 hours, providing a rapid and reliable diagnostic tool for HPV detection and typing that is amenable to automation...
  32. pmc Surveillance of childhood influenza virus infection: what is the best diagnostic method to use for archival samples?
    Brent Frisbie
    Department of Pathology, Vanderbilt University, Nashville, Tennessee, USA
    J Clin Microbiol 42:1181-4. 2004
    ..Thus, in archival specimens stored at -70 degrees C, PCR was more sensitive than viral culture. However, testing of fresh specimens had the highest yield in this study. Studies of optimal methods for specimen storage are needed...
  33. pmc Simultaneous identification of mycobacterial isolates to the species level and determination of tuberculosis drug resistance by PCR followed by electrospray ionization mass spectrometry
    Christian Massire
    Ibis Biosciences, Inc, 2251 Faraday Ave, Carlsbad, CA 92008, USA
    J Clin Microbiol 49:908-17. 2011
    ..The assay also provides information on ethambutol, fluoroquinolone, and diarylquinoline resistance and lineage-specific polymorphisms, to yield highly discriminative digital signatures potentially suitable for epidemiology tracking...
  34. pmc Assessment of Clostridium difficile infections by quantitative detection of tcdB toxin by use of a real-time cell analysis system
    Alex B Ryder
    Departments of Pathology and Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 5310, USA
    J Clin Microbiol 48:4129-34. 2010
    ..89 ng/ml) were significantly lower than those prior to the treatment (15.68 ng/ml, Wilcoxon P = 0.01). The study demonstrates that the RTCA assay provides a functional tool for the potential assessment of C. difficile infections...
  35. pmc Molecular approaches to detecting herpes simplex virus and enteroviruses in the central nervous system
    Thomas W Smalling
    Departments of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 40:2317-22. 2002
  36. ncbi request reprint Identification of the bacterial etiology of culture-negative endocarditis by amplification and sequencing of a small ribosomal RNA gene
    Irakli Khulordava
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Diagn Microbiol Infect Dis 46:9-11. 2003
    ..These results support an important role for polymerase chain reaction followed by direct sequencing to determine the etiology of culture-negative bacterial endocarditis and to guide appropriate antimicrobial therapy...
  37. pmc Simultaneous detection and high-throughput identification of a panel of RNA viruses causing respiratory tract infections
    Haijing Li
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 45:2105-9. 2007
    ..The multiplex approach enhances diagnosis through detection of respiratory viral etiologic agents in cases in which the presence of the agent was not suspected and a test was not ordered by the clinicians...
  38. pmc Comparative evaluation of Bruker Biotyper and BD Phoenix systems for identification of bacterial pathogens associated with urinary tract infections
    Yingjun Yan
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Clin Microbiol 49:3936-9. 2011
    ..9% and 99.5% to the genus level and 99.1% and 98.5% to the species level, respectively. Both systems provide reliable results, and the Biotyper system offers a rapid tool for urine bacterial isolate identification...
  39. doi request reprint Sensitive, qualitative detection of human herpesvirus-6 and simultaneous differentiation of variants A and B
    Haijing Li
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232, United States
    J Clin Virol 46:20-3. 2009
    ..The current limitations of laboratory testing for the detection of human herpesvirus virus 6 (HHV-6) in clinical specimens with low HHV-6 viral loads make this area a priority for further research and development...
  40. doi request reprint Simultaneous detection and differentiation of respiratory syncytial virus and other respiratory viral pathogens
    Susan E Sefers
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN, USA
    Methods Mol Biol 665:309-23. 2011
    ....
  41. pmc Mass spectrometry biotyper system identifies enteric bacterial pathogens directly from colonies grown on selective stool culture media
    Ying He
    Departments of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, 5310, USA
    J Clin Microbiol 48:3888-92. 2010
    ..The Biotyper system provides a rapid and simple screening tool for identifying many, but not all, suspicious colonies grown on selective media within 24 h after inoculation, which shortens test turnaround time by 2 to 3 days...
  42. doi request reprint New technology for rapid molecular diagnosis of bloodstream infections
    David J Ecker
    Ibis Biosciences, a subsidiary of Abbott Molecular, Inc, 2251 Faraday Ave, Carlsbad, CA 92008, USA
    Expert Rev Mol Diagn 10:399-415. 2010
    ..We then focus on a new method that combines nucleic acid amplification with mass spectrometry in a novel approach to molecular diagnosis of bloodstream infections...
  43. pmc Molecular diagnosis of necrotizing fasciitis by 16S rRNA gene sequencing and superantigen gene detection
    Kenneth L Muldrew
    Molecular Infectious Disease Laboratory, Vanderbilt University Hospital, 4605 TVC, Nashville, TN 37232 5310, USA
    J Mol Diagn 7:641-5. 2005
    ..This confirmed the etiology of the patient's rapid deterioration with multisystem organ failure...
  44. pmc Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2
    Hatim T Allawi
    Third Wave Technologies, Inc, 502 South Rosa Rd, Madison, WI 53719, USA
    J Clin Microbiol 44:3443-7. 2006
    ..The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens...
  45. pmc MVPlex assay for direct detection of methicillin-resistant Staphylococcus aureus in naris and other swab specimens
    Raymond P Podzorski
    Department of Pathology, Waukesha Memorial Hospital, Waukesha, Wisconsin 53188, USA
    J Clin Microbiol 46:3107-9. 2008
    ..8% and 84.4% (P = 0.002) and specificities of 95.8% and 98.6% (P < 0.05), respectively...
  46. doi request reprint Molecular assays for the detection and characterization of respiratory viruses
    Yingjun Yan
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 5310, USA
    Semin Respir Crit Care Med 32:512-26. 2011
    ....
  47. ncbi request reprint Cytokine expression in respiratory syncytial virus-infected mice as measured by quantitative reverse-transcriptase PCR
    Xinqing Deng
    Division of Infectious Diseases, Department of Medicine, Vanderbilt University Hospital, A3310 MCN, Nashville, TN 37232 2605, USA
    J Virol Methods 107:141-6. 2003
    ..TaqMan, which is rapid, sensitive and reproducible, provides an alternative tool for the quantitative analysis of cytokine mRNA expression in the murine model of RSV immunopathogenesis...
  48. pmc Characterization of a novel rapidly growing Mycobacterium species associated with sepsis
    Tao Hong
    Microbiology Laboratory, Department of Pathology, Hackensack University Medical Center, Hackensack University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07601, USA
    J Clin Microbiol 41:5650-3. 2003
    ..The name "Mycobacterium hackensackense" is proposed for this unique strain, 147-0552(T), which was deposited in the American Type Culture Collection as ATCC BAA-823(T)...
  49. ncbi request reprint Small colony variant Staphylococcus aureus multiorgan infection
    Hemant Agarwal
    Division of Pediatric Critical Care, Vanderbilt Children s Hospital, 2200 Children s Way, 5121 B Doctors Office Towers, Nashville, TN 37232, USA
    Pediatr Infect Dis J 26:269-71. 2007
    ..aureus infections after prolonged antibiotic therapy. An unusual case of multiorgan infection in the absence of prolonged antibiotic therapy in a pediatric patient is reported...
  50. pmc Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene
    C Kuyler Doyle
    Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    J Mol Diagn 7:504-10. 2005
    ..This assay provides a powerful tool for prospective medical diagnosis for human and canine ehrlichioses and for ecologic and epidemiological studies involving arthropod and mammalian hosts...
  51. ncbi request reprint Evaluation of a Digene-recommended algorithm for human papillomavirus low-positive results present in a "retest zone"
    Kenneth L Muldrew
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN, USA
    Am J Clin Pathol 127:97-102. 2007
    ..Repeating specimens in the retest zone is necessary, but a second retest does not offer advantages over the first retest...
  52. ncbi request reprint Accuracy and impact of a point-of-care rapid influenza test in young children with respiratory illnesses
    Katherine A Poehling
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tenn 37232 2504, USA
    Arch Pediatr Adolesc Med 160:713-8. 2006
    ..To determine whether a point-of-care rapid influenza test impacts the diagnostic evaluation and treatment of children with acute respiratory illnesses...
  53. doi request reprint Carbapenem resistance via the blaKPC-2 gene in Enterobacter cloacae blood culture isolate
    Andy Lo
    Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232 5310, USA
    South Med J 103:453-4. 2010
    ..To our knowledge, this is the first report of a nosocomial isolate with carbapenemase-mediated resistance causing infection in a patient from Tennessee...
  54. pmc Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system
    Yingjun Yan
    Department of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232 5310, USA
    J Clin Microbiol 49:2528-32. 2011
    ..Our data indicated that the Sepsityper specimen processing and Microflex analysis by the MALDI Biotyper system provide a rapid and reliable tool for yeast species identification directly from positive blood culture media...
  55. pmc Mycobacterium haemophilum and Histoplasma capsulatum coinfection in a renal transplant patient
    Cecilia G Clement
    Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555 0740, USA
    J Clin Microbiol 49:3425-8. 2011
    ..We report the case of a 22-year-old man who presented with a Mycobacterium haemophilum and Histoplasma capsulatum coinfection occurring 21 years after a living-donor-related renal transplant...
  56. ncbi request reprint Clinical and molecular characterization of vancomycin-resistant Enterococcus faecium strains during establishment of endemicity
    E M D'Agata
    Department of Medicine, Division of Infectious Diseases, Vanderbilt University School of Medicine, Medical Center North A 3310, 21st Ave and Garland St, Nashville, TN 37232, USA
    Clin Infect Dis 33:511-6. 2001
    ..Establishment of VREF endemicity was initially characterized by clonal spread of type O strains. Subsequently, polyclonal dissemination may have been due to microevolutionary changes among type O strains...
  57. ncbi request reprint Vancomycin-resistant enterococci among chronic hemodialysis patients: a prospective study of acquisition
    E M D'Agata
    Department of Medicine, Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN 37232 2605, USA
    Clin Infect Dis 32:23-9. 2001
    ..Hospital acquisition of VRE contributes substantially to the increasing prevalence of VRE in the chronic hemodialysis patient population...
  58. pmc Hospital-acquired Bordetella bronchiseptica infection following hematopoietic stem cell transplantation
    Elizabeth S Huebner
    Department of Medicine, Vanderbilt University Hospital, Medical Center, 4605 TVC, Nashville, TN 37232 5310, USA
    J Clin Microbiol 44:2581-3. 2006
    ..Neither patient had had direct contact with animals since transplantation. These findings strongly support nosocomial transmission of B. bronchiseptica...
  59. pmc Angiotensin type 1 receptor modulates macrophage polarization and renal injury in obesity
    Li Jun Ma
    Department of Pathology, Vanderbilt Univ Medical Center, Nashville, TN 37232 2561, USA
    Am J Physiol Renal Physiol 300:F1203-13. 2011
    ..Our data suggest obesity accelerates kidney injury, linked to augmented inflammation in adipose and kidney tissues and a proinflammatory shift in macrophage and M1/M2 balance...
  60. doi request reprint Kikuchi-Fujimoto lymphadenitis: role of parvovirus B-19, Epstein-Barr virus, human herpesvirus 6, and human herpesvirus 8
    Flavia Guimaraes Nunes Rosado
    Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Hum Pathol 44:255-9. 2013
    ..These findings do not support a role for Epstein-Barr virus, human herpesvirus 6, human herpesvirus 8, or parvovirus B19 in the pathogenesis of Kikuchi-Fujimoto lymphadenitis...
  61. pmc Absence of gastrointestinal pathogens in ileum tissue resected for necrotizing enterocolitis
    Tim Ullrich
    Department of Pediatrics, Monroe Carell Jr Children s Hospital at Vanderbilt, Vanderbilt University, Nashville, TN, USA
    Pediatr Infect Dis J 31:413-4. 2012
    ..We did not detect infectious enteritis pathogens in any of the NEC tissues and conclude that gastrointestinal pathogens are a rare cause of NEC...
  62. doi request reprint Emerging molecular assays for detection and characterization of respiratory viruses
    Wenjuan Wu
    Department of Pathology, Vanderbilt University Medical Center, Nashville, TN, USA
    Clin Lab Med 29:673-93. 2009
    ..Further validation and implementation of such emerging molecular assays in routine clinical virology services will enhance the rapid diagnosis of respiratory viral infections and improve patient care...
  63. ncbi request reprint Cytomegalovirus ventriculoencephalitis in a peripheral blood stem cell transplant recipient
    Geraldine G Miller
    Division of Infectious Diseases, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Clin Infect Dis 42:e26-9. 2006
    ....
  64. pmc Complex febrile seizures followed by complete recovery in an infant with high-titer 2009 pandemic influenza A (H1N1) virus infection
    Mandy F O'Leary
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 48:3803-5. 2010
    ..Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza virus...
  65. doi request reprint Matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database for identification of Legionella species
    Ying He
    Department of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Can J Microbiol 57:533-8. 2011
    ..More Legionella strains, especially the non-L. pneumophila strains, need to be included in the current Biotyper database to cover varieties of Legionella species and to increase identification accuracy...
  66. pmc Necrotizing fasciitis from Vibrio vulnificus in a patient with undiagnosed hepatitis and cirrhosis
    Kenneth L Muldrew
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    J Clin Microbiol 45:1058-62. 2007
    ..Significant risk factors for severe disease include preexisting liver disease. We report a case of Vibrio vulnificus necrotizing fasciitis in a patient with previously undiagnosed chronic hepatitis and cirrhosis...
  67. pmc Increased detectability of plasma HIV-1 RNA after introduction of a new assay and altered specimen-processing procedures
    Peter F Rebeiro
    Division of Infectious Diseases, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2582, USA
    Clin Infect Dis 47:1354-7. 2008
    ..This artifact is attributable to shipping frozen plasma in primary plasma preparation tubes and is not caused by the HIV-1 RNA detection assay; it can be avoided by shipping plasma in a secondary tube...
  68. ncbi request reprint Community dissemination of vancomycin-resistant Enterococcus faecium
    J Jirjis
    Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Am J Infect Control 29:316-20. 2001
    ..CONCLUSIONS: VRE was recovered from nonhospitalized patients. Physicians should be alert to the potential of VRE transmission in the ambulatory care setting...
  69. pmc Detection of viruses in human adenoid tissues by use of multiplex PCR
    Masatoki Sato
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Clin Microbiol 47:771-3. 2009
    ..Our results suggest that persistent/latent viral infection in the respiratory tract confounds interpretation of the association of pathogen detection by PCR with acute respiratory infection in these sources...
  70. pmc Prevalence and management of invalid GeneXpert enterovirus results obtained with cerebrospinal fluid samples: a 2-year study
    Susan E Sefers
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Clin Microbiol 47:3008-10. 2009
    ..Invalid GXEA result rates were markedly diminished by 82.1% and 96.0% and test sensitivities were minimally decreased by 1.7% and 3.6% when these specimens were tested at a 1:5 dilution and after a freeze-thaw cycle, respectively...
  71. ncbi request reprint Plasminogen activator inhibitor-1 modulates adipocyte differentiation
    Xiubin Liang
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232 2561, USA
    Am J Physiol Endocrinol Metab 290:E103-E113. 2006
    ..We postulate that these PAI-1 effects on adipocytes may, at least in part, be mediated via modulation of plasmin activity and extracellular matrix components...
  72. ncbi request reprint High rate of false-negative results of the rectal swab culture method in detection of gastrointestinal colonization with vancomycin-resistant enterococci
    Erika M C D'Agata
    Department of Medicine, Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN, USA
    Clin Infect Dis 34:167-72. 2002
    ..001). Antibiotic exposure was significantly associated with higher VRE stool densities (P<.001). The high false-negative rate of the RS may be contributing to the continued increase in the prevalence of VRE...
  73. ncbi request reprint Comparison of pulsed-field gel electrophoresis and amplified fragment-length polymorphism for epidemiological investigations of common nosocomial pathogens
    M M Gerrits
    Division of Infectious Diseases, Vanderbilt University, Nashville, Tennessee, USA
    Infect Control Hosp Epidemiol 22:550-4. 2001
    ..CONCLUSION: Molecular typing by AFLP is comparable to PFGE for A baumannii and P aeruginosa isolates. For VRE isolates, however, PFGE remains the method of choice...
  74. pmc Immune mediators of protective and pathogenic immune responses in patients with mild and fatal human monocytotropic ehrlichiosis
    Nahed Ismail
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA
    BMC Immunol 13:26. 2012
    ..However, there are no human studies that examine the correlates of resistance or susceptibility to severe and fatal HME...
  75. ncbi request reprint PCR detection of Histoplasma capsulatum var. capsulatum in whole blood of a renal transplant patient with disseminated histoplasmosis
    Julianne Qualtieri
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Ann Clin Lab Sci 39:409-12. 2009
    ..Blood fungal cultures in this case remained negative, suggesting that molecular assays may facilitate the laboratory diagnosis of disseminated histoplasmosis...
  76. ncbi request reprint Human ehrlichiosis in transplant recipients
    L D Thomas
    Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA
    Am J Transplant 7:1641-7. 2007
    ..Ehrlichia infections can occur in transplant recipients who live in an endemic area. With prompt treatment, the infected transplant recipients in our study had similar, favorable outcomes compared to immunocompetent patients...
  77. pmc Early infant human immunodeficiency virus type 1 detection suitable for resource-limited settings with multiple circulating subtypes by use of nested three-monoplex DNA PCR and dried blood spots
    Qi Zhang
    National Center for Women and Children s Health, China Center for Disease Control and Prevention, Beijing 100013, People s Republic of China
    J Clin Microbiol 46:721-6. 2008
    ..7% at 1 to 2 months, and 100% at 3 to 6 months of age. The HIV-1 DNA N3M-PCR assay on DBSs offers a simple and affordable approach for early infant HIV-1 diagnosis in regions with diverse HIV-1 circulating subtypes...
  78. pmc Comparative evaluation of three commercially available methodologies for hepatitis C virus genotyping
    Ted E Schutzbank
    CompuNet Clinical Laboratories, 2308 Sandridge Rd, Dayton, OH 45439, USA
    J Clin Microbiol 44:3797-8. 2006
    ..Genotypes were determined for 136 of 137 specimens by the Bayer method, 121 of 137 specimens by the Invader assay, and only 77 of 137 specimens by the Abbott assay. All reported genotypes were concordant by all three methods...
  79. pmc Application of isothermal helicase-dependent amplification with a disposable detection device in a simple sensitive stool test for toxigenic Clostridium difficile
    Wing Huen A Chow
    BioHelix Corporation, 32 Tozer Road, Beverly, MA 01915, USA
    J Mol Diagn 10:452-8. 2008
    ....
  80. pmc Primer sequence modification enhances hepatitis C virus genotype coverage
    Yi Wei Tang
    J Clin Microbiol 43:3576-7. 2005
  81. pmc Prospective evaluation of rapid antigen tests for diagnosis of respiratory syncytial virus and human metapneumovirus infections
    Jaber Aslanzadeh
    Hartford Hospital and Clinical Laboratory Partners, Hartford, Connecticut 06102, USA
    J Clin Microbiol 46:1682-5. 2008
    ..Similarly, both hMPV DFAs are highly specific in comparison to the results of RT-PCR, but their sensitivities await further improvements...
  82. pmc Clinical use of 16S rRNA gene sequencing to identify Mycoplasma felis and M. gateae associated with feline ulcerative keratitis
    Larry D Gray
    TriHealth Laboratories and Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Clin Microbiol 43:3431-4. 2005
    ..Identification of the pathogens allowed the use of less empirical and more organism-specific therapy...
  83. pmc Prevalence of and molecular basis for tuberculosis drug resistance in the Republic of Georgia: validation of a QIAplex system for detection of drug resistance-related mutations
    Medea Gegia
    Georgian Foundation against Tuberculosis and Lung Diseases, National Center of Tuberculosis and Lung Diseases, Tbilisi, Georgia, USA
    Antimicrob Agents Chemother 52:725-9. 2008
    ..05). Simultaneous detection of 24 common drug resistance-related mutations provides a molecular tool for studying and monitoring M. tuberculosis resistance mechanism and epidemiology...
  84. pmc Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum
    Louis Flamand
    Rheumatology and Immunology Research Center, CHUL Research Center, Room T1 49, 2705 Laurier Blvd, Quebec, Quebec, Canada G1V 4G2
    J Clin Microbiol 46:2700-6. 2008
    ..To provide better homogeneity between the results from the different laboratories working on HHV-6, we propose that investigators interested in quantifying HHV-6 in clinical samples adopt one of these assays...
  85. pmc Acinetobacter septicus sp. nov. association with a nosocomial outbreak of bacteremia in a neonatal intensive care unit
    Abdullah Kilic
    Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018 Ankara, Turkey
    J Clin Microbiol 46:902-8. 2008
    ..We propose the designation of Acinetobacter septicus sp. nov. for these isolates, with isolate AK001 as the type strain...
  86. ncbi request reprint Dual infections of the central nervous system with Epstein-Barr virus
    Adriana Weinberg
    Department of Pediatrics, University of Colorado Medical School, Denver, CO 80262, USA
    J Infect Dis 191:234-7. 2005
    ..EBV lytic-cycle mRNA was detected in the cerebrospinal fluid of 5 of 6 tested samples, indicating EBV replication in the CNS during coinfection...
  87. pmc Genetic analysis of Crimean-Congo hemorrhagic fever virus in Russia
    Lyudmila Yashina
    State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, 630559, Russia
    J Clin Microbiol 41:860-2. 2003
    ..0%). The strains differed significantly from CCHF viruses from other regions of the world (nucleotide sequence diversity, 10.3 to 20.4%), suggesting that CCHF virus strains recovered in the European part of Russia form a distinct group...
  88. pmc Enterococcus faecium-related outbreak with molecular evidence of transmission from pigs to humans
    Hong Zhou Lu
    Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, People s Republic of China
    J Clin Microbiol 40:913-7. 2002
    ..faecium isolates displayed indistinguishable antibiotic susceptibility and pulsed-field gel electrophoresis patterns. These data strongly suggest the spread of an outbreak of E. faecium-related sepsis from pigs to humans...
  89. pmc Clonal spread of serogroup W135 meningococcal disease in Turkey
    Abdullah Kilic
    Gulhane Military Medical Academy, Ankara, Turkey
    J Clin Microbiol 44:222-4. 2006
    ..These data demonstrate that serogroup W135 meningococci with different genotypes, including the Hajj epidemic strain, are endemic in Turkey...
  90. pmc PCR Enhances acid-fast bacillus stain-based rapid detection of Mycobacterium tuberculosis
    Yi Wei Tang
    J Clin Microbiol 42:1849-50. 2004