CHARLES SINGLETON

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. pmc Loss of the Histidine Kinase DhkD Results in Mobile Mounds during Development of Dictyostelium discoideum
    Charles K Singleton
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America
    PLoS ONE 8:e75618. 2013
  2. pmc eIF2α kinases regulate development through the BzpR transcription factor in Dictyostelium discoideum
    Charles K Singleton
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America
    PLoS ONE 7:e32500. 2012
  3. pmc IfkA, a presumptive eIF2 alpha kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size
    Rui Fang
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville TN 37235 1634, USA
    BMC Dev Biol 3:3. 2003
  4. pmc Subcellular localization of ammonium transporters in Dictyostelium discoideum
    Janet H Kirsten
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
    BMC Cell Biol 9:71. 2008
  5. pmc Mitochondria from cultured cells derived from normal and thiamine-responsive megaloblastic anemia individuals efficiently import thiamine diphosphate
    Qilin Song
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville TN 37235 1634, USA
    BMC Biochem 3:8. 2002
  6. ncbi request reprint Molecular mechanisms of thiamine utilization
    C K Singleton
    Department of Biological Science, Vanderbilt University, Nashville, TN 37235, USA
    Curr Mol Med 1:197-207. 2001
  7. ncbi request reprint The histidine kinase dhkC regulates the choice between migrating slugs and terminal differentiation in Dictyostelium discoideum
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee, 37235, USA
    Dev Biol 203:345-57. 1998
  8. pmc Function of ammonium transporter A in the initiation of culmination of development in Dictyostelium discoideum
    Charles K Singleton
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, Tennessee 37235 1634, USA
    Eukaryot Cell 5:991-6. 2006
  9. ncbi request reprint Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation
    J J Wang
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Biochim Biophys Acta 1341:165-72. 1997
  10. ncbi request reprint Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Gene 199:111-21. 1997

Research Grants

  1. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 1999
  2. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 2000
  3. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 2001
  4. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 2002

Collaborators

Detail Information

Publications18

  1. pmc Loss of the Histidine Kinase DhkD Results in Mobile Mounds during Development of Dictyostelium discoideum
    Charles K Singleton
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America
    PLoS ONE 8:e75618. 2013
    ..Hence, all of the histidine kinases characterized to date regulate developmental processes through modulating cAMP production. Here we investigate the function of the histidine kinase DhkD...
  2. pmc eIF2α kinases regulate development through the BzpR transcription factor in Dictyostelium discoideum
    Charles K Singleton
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America
    PLoS ONE 7:e32500. 2012
    ..Cells possessing an eIF2α that cannot be phosphorylated (BS167) show abnormalities in growth and development. We sought to identify a bZIP protein in Dictyostelium whose production is controlled by the eIF2α regulatory system...
  3. pmc IfkA, a presumptive eIF2 alpha kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size
    Rui Fang
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville TN 37235 1634, USA
    BMC Dev Biol 3:3. 2003
    ..Two eIF2alpha kinases were identified in Dictyostelium and studied herein...
  4. pmc Subcellular localization of ammonium transporters in Dictyostelium discoideum
    Janet H Kirsten
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
    BMC Cell Biol 9:71. 2008
    ....
  5. pmc Mitochondria from cultured cells derived from normal and thiamine-responsive megaloblastic anemia individuals efficiently import thiamine diphosphate
    Qilin Song
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville TN 37235 1634, USA
    BMC Biochem 3:8. 2002
    ..Previous reports indicate that ThDP can also be taken up by rat mitochondria, but the kinetic constants associated with such uptake seemed not to be physiologically relevant...
  6. ncbi request reprint Molecular mechanisms of thiamine utilization
    C K Singleton
    Department of Biological Science, Vanderbilt University, Nashville, TN 37235, USA
    Curr Mol Med 1:197-207. 2001
    ....
  7. ncbi request reprint The histidine kinase dhkC regulates the choice between migrating slugs and terminal differentiation in Dictyostelium discoideum
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee, 37235, USA
    Dev Biol 203:345-57. 1998
    ..Additionally, dhkC- spores were found to be defective in germination, indicating a role for the DHKC signaling pathway in activating spore germination...
  8. pmc Function of ammonium transporter A in the initiation of culmination of development in Dictyostelium discoideum
    Charles K Singleton
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, Tennessee 37235 1634, USA
    Eukaryot Cell 5:991-6. 2006
    ..These findings lead to the proposal that AmtA functions in ammonia sensing as an activator of the DhkC phosphorelay in response to perceived high ammonia levels...
  9. ncbi request reprint Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation
    J J Wang
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Biochim Biophys Acta 1341:165-72. 1997
    ..In support of this interpretation, binding of cofactor by wild type apo-transketolase required the presence of magnesium. Additionally, monomeric apo-his-transketolase required both magnesium and cofactor binding for dimer formation...
  10. ncbi request reprint Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Gene 199:111-21. 1997
    ..The level of transporter mRNA was found to be rapidly and dramatically reduced by the addition of thiamine to the growth medium...
  11. ncbi request reprint Conserved residues are functionally distinct within transketolases of different species
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Biochemistry 35:15865-9. 1996
    ..In other TPP-utilizing enzymes, the equivalent residue of Q428 is a histidine and is thought to deprotonate the 4'-amino group...
  12. ncbi request reprint Thiamine pyrophosphate-requiring enzymes are altered during pyrithiamine-induced thiamine deficiency in cultured human lymphoblasts
    S R Pekovich
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37325, USA
    J Nutr 126:1791-8. 1996
    ....
  13. ncbi request reprint Cloning of human transketolase cDNAs and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals
    B A McCool
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235
    J Biol Chem 268:1397-404. 1993
    ..Instead, the underlying mechanism must be extragenic and may be a result of differences in post-translational processing/modification of the transketolase polypeptide...
  14. pmc eIF2α kinases control chalone production in Dictyostelium discoideum
    Robert L Bowman
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235 1634, USA
    Eukaryot Cell 10:494-501. 2011
    ..Both proteins are upregulated at the culmination of development, and this enhanced production is lacking in a strain that possesses a nonphosphorylatable eIF2α...
  15. ncbi request reprint Ammonium transporter C of Dictyostelium discoideum is required for correct prestalk gene expression and for regulating the choice between slug migration and culmination
    Janet H Kirsten
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
    Dev Biol 287:146-56. 2005
    ..The results suggest that the lack of nuclear translocation of STATa results from low cAMP due to a misregulated and overactive DhkC phosphorelay in the amtC null strain...
  16. ncbi request reprint Disruption of the ifkA and ifkB genes results in altered cell adhesion, morphological defects and a propensity to form pre-stalk O cells during development of Dictyostelium
    Meena Rai
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235 1634, USA
    Differentiation 74:583-95. 2006
    ....
  17. ncbi request reprint Temporal and spatial expression of ammonium transporter genes during growth and development of Dictyostelium discoideum
    Susan C Follstaedt
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
    Differentiation 71:557-66. 2003
    ..Indeed, amtC null cells have a slugger phenotype, suggesting AmtC functions in the signaling pathway underlying the mechanics of this choice...
  18. ncbi request reprint The role of thiamine deficiency in alcoholic brain disease
    Peter R Martin
    Vanderbilt University School of Medicine, Vanderbilt Addiction Center, Nashville, Tennessee, USA
    Alcohol Res Health 27:134-42. 2003
    ..People differ in their susceptibility to thiamine deficiency, however, and different brain regions also may be more or less sensitive to this condition...

Research Grants4

  1. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 1999
    ..Finally genetic screens for suppressers or synthetic phenotypes will identify new and perhaps novel components of the regulatory pathway, including upstream inputs, phosphorelay regulators, and downstream effectors. ..
  2. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 2000
    ..Finally genetic screens for suppressers or synthetic phenotypes will identify new and perhaps novel components of the regulatory pathway, including upstream inputs, phosphorelay regulators, and downstream effectors. ..
  3. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 2001
    ..Finally genetic screens for suppressers or synthetic phenotypes will identify new and perhaps novel components of the regulatory pathway, including upstream inputs, phosphorelay regulators, and downstream effectors. ..
  4. REGULATION OF DEVELOPMENT BY PHOSPHORELAY SIGNALING
    CHARLES SINGLETON; Fiscal Year: 2002
    ..Finally genetic screens for suppressers or synthetic phenotypes will identify new and perhaps novel components of the regulatory pathway, including upstream inputs, phosphorelay regulators, and downstream effectors. ..