C K Singleton

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi Conserved residues are functionally distinct within transketolases of different species
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Biochemistry 35:15865-9. 1996
  2. ncbi Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Gene 199:111-21. 1997
  3. ncbi Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation
    J J Wang
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Biochim Biophys Acta 1341:165-72. 1997
  4. ncbi Thiamine pyrophosphate-requiring enzymes are altered during pyrithiamine-induced thiamine deficiency in cultured human lymphoblasts
    S R Pekovich
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37325, USA
    J Nutr 126:1791-8. 1996
  5. ncbi Cloning of human transketolase cDNAs and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals
    B A McCool
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235
    J Biol Chem 268:1397-404. 1993
  6. ncbi Molecular mechanisms of thiamine utilization
    C K Singleton
    Department of Biological Science, Vanderbilt University, Nashville, TN 37235, USA
    Curr Mol Med 1:197-207. 2001
  7. ncbi The histidine kinase dhkC regulates the choice between migrating slugs and terminal differentiation in Dictyostelium discoideum
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee, 37235, USA
    Dev Biol 203:345-57. 1998
  8. ncbi DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor
    C K Singleton
    Nucleic Acids Res 8:1551-60. 1980

Collaborators

Detail Information

Publications8

  1. ncbi Conserved residues are functionally distinct within transketolases of different species
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Biochemistry 35:15865-9. 1996
    ..In other TPP-utilizing enzymes, the equivalent residue of Q428 is a histidine and is thought to deprotonate the 4'-amino group...
  2. ncbi Identification and characterization of the thiamine transporter gene of Saccharomyces cerevisiae
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Gene 199:111-21. 1997
    ..The level of transporter mRNA was found to be rapidly and dramatically reduced by the addition of thiamine to the growth medium...
  3. ncbi Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation
    J J Wang
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA
    Biochim Biophys Acta 1341:165-72. 1997
    ..In support of this interpretation, binding of cofactor by wild type apo-transketolase required the presence of magnesium. Additionally, monomeric apo-his-transketolase required both magnesium and cofactor binding for dimer formation...
  4. ncbi Thiamine pyrophosphate-requiring enzymes are altered during pyrithiamine-induced thiamine deficiency in cultured human lymphoblasts
    S R Pekovich
    Department of Molecular Biology, Vanderbilt University, Nashville, TN 37325, USA
    J Nutr 126:1791-8. 1996
    ....
  5. ncbi Cloning of human transketolase cDNAs and comparison of the nucleotide sequence of the coding region in Wernicke-Korsakoff and non-Wernicke-Korsakoff individuals
    B A McCool
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235
    J Biol Chem 268:1397-404. 1993
    ..Instead, the underlying mechanism must be extragenic and may be a result of differences in post-translational processing/modification of the transketolase polypeptide...
  6. ncbi Molecular mechanisms of thiamine utilization
    C K Singleton
    Department of Biological Science, Vanderbilt University, Nashville, TN 37235, USA
    Curr Mol Med 1:197-207. 2001
    ....
  7. ncbi The histidine kinase dhkC regulates the choice between migrating slugs and terminal differentiation in Dictyostelium discoideum
    C K Singleton
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee, 37235, USA
    Dev Biol 203:345-57. 1998
    ..Additionally, dhkC- spores were found to be defective in germination, indicating a role for the DHKC signaling pathway in activating spore germination...
  8. ncbi DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor
    C K Singleton
    Nucleic Acids Res 8:1551-60. 1980
    ....