- Isolation and characterization of new Saccharomyces cerevisiae mutants perturbed in nuclear pore complex assemblyKathryn J Ryan
Department of Cell Biology and Physiology, Washington University School of Medicine, St, Louis, MO 63110, USA
BMC Genet 3:17. 2002..To identify such mutants, a bank of temperature sensitive strains was generated and examined by fluorescence microscopy for mislocalization of GFP-tagged nups at the non-permissive temperature...
- The Ran GTPase cycle is required for yeast nuclear pore complex assemblyKathryn J Ryan
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
J Cell Biol 160:1041-53. 2003..We propose a model wherein a Ran-mediated vesicular fusion step is required for NPC assembly into intact nuclear envelopes...
- The karyopherin Kap95 regulates nuclear pore complex assembly into intact nuclear envelopes in vivoKathryn J Ryan
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232 8240, USA
Mol Biol Cell 18:886-98. 2007..We conclude that Kap95 serves as a key regulator of NPC assembly into intact NEs. Furthermore, both Kap95 and Ran may provide spatial cues necessary for targeting of vesicular intermediates in de novo NPC assembly...