L J Roberts

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi New developments in the isoprostane pathway: identification of novel highly reactive gamma-ketoaldehydes (isolevuglandins) and characterization of their protein adducts
    L J Roberts
    Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, Tennessee, 37232 6602, USA
    FASEB J 13:1157-68. 1999
  2. ncbi Measurement of F(2)-isoprostanes as an index of oxidative stress in vivo
    L J Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
    Free Radic Biol Med 28:505-13. 2000
  3. ncbi Products of the isoprostane pathway: unique bioactive compounds and markers of lipid peroxidation
    L Jackson Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Cell Mol Life Sci 59:808-20. 2002
  4. ncbi Measurement of F(2)-isoprostanes unveils profound oxidative stress in aged rats
    L J Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 287:254-6. 2001
  5. ncbi Formation of isoprostane-like compounds (neuroprostanes) in vivo from docosahexaenoic acid
    L J Roberts
    Departments of Pharmacology, Pathology, and Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 273:13605-12. 1998
  6. ncbi A stable isotope dilution mass spectrometric assay for the major urinary metabolite of PGD2
    J D Morrow
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602
    Adv Prostaglandin Thromboxane Leukot Res 21:315-8. 1991
  7. ncbi Interactions between apolipoprotein E gene and dietary alpha-tocopherol influence cerebral oxidative damage in aged mice
    E E Reich
    Departments of Pathology, Medicine, and Pharmacology and Center for Molecular Neurosciences, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Neurosci 21:5993-9. 2001
  8. ncbi The magnitude of brain lipid peroxidation correlates with the extent of degeneration but not with density of neuritic plaques or neurofibrillary tangles or with APOE genotype in Alzheimer's disease patients
    T J Montine
    Department of Medicine, Center for Molecular Neurosciences, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Am J Pathol 155:863-8. 1999
  9. ncbi The isoprostanes: unique prostaglandin-like products of free-radical-initiated lipid peroxidation
    J D Morrow
    Department of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    Drug Metab Rev 31:117-39. 1999
  10. ncbi Brain regional quantification of F-ring and D-/E-ring isoprostanes and neuroprostanes in Alzheimer's disease
    E E Reich
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Am J Pathol 158:293-7. 2001

Detail Information

Publications88

  1. ncbi New developments in the isoprostane pathway: identification of novel highly reactive gamma-ketoaldehydes (isolevuglandins) and characterization of their protein adducts
    L J Roberts
    Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, Tennessee, 37232 6602, USA
    FASEB J 13:1157-68. 1999
    ..Elucidation of the nature of the adducts formed by IsoLGs provides the basis to explore the formation of IsoLGs in vivo and investigate the potential biological ramifications of their formation in settings of oxidant injury...
  2. ncbi Measurement of F(2)-isoprostanes as an index of oxidative stress in vivo
    L J Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
    Free Radic Biol Med 28:505-13. 2000
    ..Recently, we developed an assay for a urinary metabolite of F(2)-IsoPs, which should provide a valuable noninvasive integrated approach to assess total endogenous production of F(2)-IsoPs in large clinical studies...
  3. ncbi Products of the isoprostane pathway: unique bioactive compounds and markers of lipid peroxidation
    L Jackson Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Cell Mol Life Sci 59:808-20. 2002
    ....
  4. ncbi Measurement of F(2)-isoprostanes unveils profound oxidative stress in aged rats
    L J Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 287:254-6. 2001
    ..9-fold (range 15.8 to 50.0-fold). These findings unveil profound oxidative stress in aged rats which adds considerable support for the free radical theory of aging...
  5. ncbi Formation of isoprostane-like compounds (neuroprostanes) in vivo from docosahexaenoic acid
    L J Roberts
    Departments of Pharmacology, Pathology, and Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 273:13605-12. 1998
    ..Furthermore, the formation of F4-neuroprostane-containing aminophospholipids might adversely effect neuronal function as a result of alterations they induce in the biophysical properties of neuronal membranes...
  6. ncbi A stable isotope dilution mass spectrometric assay for the major urinary metabolite of PGD2
    J D Morrow
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602
    Adv Prostaglandin Thromboxane Leukot Res 21:315-8. 1991
    ..2. The finding that increased urinary excretion of this metabolite occurs in a number of clinical situations suggests that the assay may prove to be a valuable tool to explore the role of PGD2 in the pathophysiology of human disease...
  7. ncbi Interactions between apolipoprotein E gene and dietary alpha-tocopherol influence cerebral oxidative damage in aged mice
    E E Reich
    Departments of Pathology, Medicine, and Pharmacology and Center for Molecular Neurosciences, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Neurosci 21:5993-9. 2001
    ..These results demonstrated that the interaction of gender, inherited susceptibilities, and dietary alpha-tocopherol contributed differently to oxidative damage to cerebral AA and DHA in aged mice...
  8. ncbi The magnitude of brain lipid peroxidation correlates with the extent of degeneration but not with density of neuritic plaques or neurofibrillary tangles or with APOE genotype in Alzheimer's disease patients
    T J Montine
    Department of Medicine, Center for Molecular Neurosciences, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Am J Pathol 155:863-8. 1999
    ....
  9. ncbi The isoprostanes: unique prostaglandin-like products of free-radical-initiated lipid peroxidation
    J D Morrow
    Department of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    Drug Metab Rev 31:117-39. 1999
    ..With continued research in this area, we believe that much new information will emerge that will open up additional important new areas for future investigation...
  10. ncbi Brain regional quantification of F-ring and D-/E-ring isoprostanes and neuroprostanes in Alzheimer's disease
    E E Reich
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Am J Pathol 158:293-7. 2001
    ....
  11. ncbi Free radical-induced generation of isoprostanes in vivo. Evidence for the formation of D-ring and E-ring isoprostanes
    J D Morrow
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602
    J Biol Chem 269:4317-26. 1994
    ..Further understanding of the biological consequences of the formation of these novel compounds and factors that influence their formation may provide valuable insights into the pathophysiology of oxidant injury...
  12. ncbi Comparison of formation of D2/E2-isoprostanes and F2-isoprostanes in vitro and in vivo--effects of oxygen tension and glutathione
    J D Morrow
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    Arch Biochem Biophys 353:160-71. 1998
    ..These studies indicate that GSH promotes F2-IsoP formation and diminishes D2/E2-IsoP levels in vitro and in vivo by causing reduction of IsoP endoperoxides...
  13. ncbi Allergen-induced synthesis of F(2)-isoprostanes in atopic asthmatics. Evidence for oxidant stress
    R Dworski
    Center for Lung Research and Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tenessee 37232 2650, USA
    Am J Respir Crit Care Med 160:1947-51. 1999
    ..007). The increase was inhibited by pretreatment of the subjects with inhaled corticosteroids. These findings provide a new evidence for a role for ROS and lipid peroxidation in allergen-induced airway inflammation...
  14. ncbi Quantification of the major urinary metabolite of prostaglandin D2 by a stable isotope dilution mass spectrometric assay
    J D Morrow
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602
    Anal Biochem 193:142-8. 1991
    ..Thus, this assay provides a sensitive and accurate method to assess endogenous production of prostaglandin D2 as a means to explore the pathophysiological role of prostaglandin D2 in human disease...
  15. ncbi Isomeric prostaglandin F2 compounds arising from prostaglandin D2: a family of icosanoids produced in vivo in humans
    D F Wendelborn
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232
    Proc Natl Acad Sci U S A 85:304-8. 1988
    ....
  16. ncbi Novel eicosanoids. Isoprostanes and related compounds
    L J Roberts
    Division of Clinical Pharmacology, Vanderbilt University, Nashville, TN, USA
    Methods Mol Biol 120:257-85. 1999
    ....
  17. ncbi Characterization of the lysyl adducts formed from prostaglandin H2 via the levuglandin pathway
    O Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 38:9389-96. 1999
    ..These findings provide a basis for investigating protein modification induced by oxygenation of arachidonic acid by the cyclooxygenases...
  18. ncbi Improved diagnosis of mastocytosis by measurement of the major urinary metabolite of prostaglandin D2
    J D Morrow
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232 6602, USA
    J Invest Dermatol 104:937-40. 1995
    ..These data indicate that quantification of the urinary excretion of PGD-M is a more sensitive biochemical diagnostic indicator of mastocytosis than is the quantification of NT-methylhistamine...
  19. ncbi Formation of highly reactive gamma-ketoaldehydes (neuroketals) as products of the neuroprostane pathway
    N Bernoud-Hubac
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232-6602, USA
    J Biol Chem 276:30964-70. 2001
    ....
  20. ncbi Actions of the E2-isoprostane, 8-ISO-PGE2, on the platelet thromboxane/endoperoxide receptor in humans and rats: additional evidence for the existence of a unique isoprostane receptor
    A W Longmire
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232 6602
    Prostaglandins 48:247-56. 1994
    ..3 x 10(-7) M) with an IC50 of 5 x 10(-7) M and 5 x 10(-6) M, respectively. 8-iso-PGE2 also inhibited platelet aggregation induced by arachidonic acid but not by ADP. Similarly in rat platelets, 8-iso-PGE2 alone...
  21. ncbi Assessment of oxidant stress in allergic asthma by measurement of the major urinary metabolite of F2-isoprostane, 15-F2t-IsoP (8-iso-PGF2alpha)
    R Dworski
    Department of Critical Care Medicine, Vanderbilt University School of Medicine, T 1217 MCN, Nashville, TN 37232 2650, USA
    Clin Exp Allergy 31:387-90. 2001
    ..No increase in the urinary excretion of 15-F2t-IsoP-M occurred after inhalation of methacholine. Allergen challenge causes an oxidant injury in human atopic asthmatics. 15-F2t-IsoP-M is a valuable marker of oxidant stress in vivo...
  22. ncbi Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid
    O Boutaud
    Department of Pharmacology, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 40:6948-55. 2001
    ..The reactivity of the PGH-synthase adducts themselves is demonstrated by the formation of intermolecular cross-links...
  23. ncbi Increased cerebral cortical lipid peroxidation and abnormal phospholipids in aged homozygous apoE-deficient C57BL/6J mice
    T J Montine
    Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Exp Neurol 158:234-41. 1999
    ....
  24. ncbi Evidence that histamine is the causative toxin of scombroid-fish poisoning
    J D Morrow
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602
    N Engl J Med 324:716-20. 1991
    ..Whether histamine is the causative toxin, however, has remained in question. To address this issue, we investigated whether histamine homeostasis is altered in poisoned people...
  25. ncbi Selective gamma-ketoaldehyde scavengers protect Nav1.5 from oxidant-induced inactivation
    T Nakajima
    Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville, TN 37232 0413, USA
    J Mol Cell Cardiol 48:352-9. 2010
    ....
  26. ncbi Increased formation of thromboxane in vivo in humans with mastocytosis
    J D Morrow
    Department of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    J Invest Dermatol 113:93-7. 1999
    ....
  27. ncbi Plasma F2-isoprostane levels are elevated in chronic hemodialysis patients
    T A Ikizler
    Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2372, USA
    Clin Nephrol 58:190-7. 2002
    ..Further, hemodialysis membrane bioincompatibility can contribute to increased oxidative stress and prevalence of inflammation...
  28. ncbi Quantification of dinor, dihydro metabolites of F2-isoprostanes in urine by liquid chromatography/tandem mass spectrometry
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, TN 27232, USA
    Anal Biochem 348:185-91. 2006
    ..77y = 67.2x-0.5). The levels of F2-IsoP-M detected in spot urines from 40 normal subjects were 38.1+/-19.1 ng/mg creatinine (mean+/-SD). This method provides an accurate and rapid assay to assess oxidative status in vivo...
  29. ncbi Release of free F2-isoprostanes from esterified phospholipids is catalyzed by intracellular and plasma platelet-activating factor acetylhydrolases
    Diana M Stafforini
    Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112 5550, USA
    J Biol Chem 281:4616-23. 2006
    ..Our results suggested that PAF acetylhydrolases play key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo...
  30. ncbi Oxidant stress modulates murine allergic airway responses
    Megha Talati
    Department of Medicine, T 1217 MCN, Vanderbilt University, Nashville, TN 37232, USA
    Free Radic Biol Med 40:1210-9. 2006
    ..Oxidant stress plays a role in the production of airway responsiveness...
  31. ncbi Oxidative mediated lipid peroxidation recapitulates proarrhythmic effects on cardiac sodium channels
    Koji Fukuda
    Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville, TN, USA
    Circ Res 97:1262-9. 2005
    ..These data suggest Na+ channel dysfunction evoked by lipid peroxidation is a candidate mechanism for ischemia-related conduction abnormalities and arrhythmias...
  32. ncbi Effects of age and caloric restriction on lipid peroxidation: measurement of oxidative stress by F2-isoprostane levels
    Walter F Ward
    Department of Physiology MSC 7756, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX 78229 3900, USA
    J Gerontol A Biol Sci Med Sci 60:847-51. 2005
    ....
  33. ncbi The biochemistry of the isoprostane, neuroprostane, and isofuran Pathways of lipid peroxidation
    L Jackson Roberts
    Department of Pharmacology, 522 RRB, Vanderbilt University, Nashville, TN 37232 6602, USA
    Brain Pathol 15:143-8. 2005
    ..Oxygen concentration differentially modulates the formation of isoprostanes and isofurans. As oxygen concentrations increase, the formation of isofurans is favored whereas the formation of isoprostanes becomes disfavored...
  34. ncbi Degree of heteroplasmy reflects oxidant damage in a large family with the mitochondrial DNA A8344G mutation
    Jeffrey A Canter
    Center for Human Genetics Research, Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 519 Light Hall, Nashville, TN 37232 0700, USA
    Free Radic Biol Med 38:678-83. 2005
    ..The most important novel finding from this family is that some currently asymptomatic individuals with moderate heteroplasmy have evidence of ongoing free-radical mediated oxidant injury...
  35. ncbi Isoprostanes: markers and mediators of oxidative stress
    Paolo Montuschi
    Department of Pharmacology, School of Medicine, Catholic University of the Sacred Heart, Rome, Italy
    FASEB J 18:1791-800. 2004
    ..Products of the isoprostane pathway have been found to exert potent biological actions and therefore may participate as physiological mediators of disease...
  36. ncbi Retardation of atherosclerosis by overexpression of catalase or both Cu/Zn-superoxide dismutase and catalase in mice lacking apolipoprotein E
    Hong Yang
    Department of Pathology, Meharry Medical College, Nashville, Tenn 37208, USA
    Circ Res 95:1075-81. 2004
    ..These observations implied that endogenously produced hydrogen peroxide, but not superoxide anions, contributed to the formation of oxidized lipids and the development of atherosclerosis in ApoE-/- mice...
  37. ncbi Transgenic mice overexpressing glutathione peroxidase 4 are protected against oxidative stress-induced apoptosis
    Qitao Ran
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 7400 Merton Mintor Boulevard, San Antonio, TX 78229, USA
    J Biol Chem 279:55137-46. 2004
    ..These data demonstrate that Gpx4 plays a role in vivo in the mechanism of apoptosis induced by oxidative stress that most likely occurs through oxidative damage to mitochondrial phospholipids such as cardiolipin...
  38. ncbi Covalent binding of isoketals to ethanolamine phospholipids
    Nathalie Bernoud-Hubac
    INSERM U585, Physiopathologie des Lipides et Membranes, INSA Lyon, 69621 Villeurbanne, France
    Free Radic Biol Med 37:1604-11. 2004
    ..These studies identify in vitro novel isoketal adducts. This provides the basis to explore the formation of isoketal-aminophospholipid adducts in vivo and the biological consequences of the formation of these adducts...
  39. ncbi Vitamin C fails to protect amino acids and lipids from oxidation during acute inflammation
    Joseph P Gaut
    Department of Surgery, Washington University, St Louis, MO 63110, USA
    Free Radic Biol Med 40:1494-501. 2006
    ..To examine the oxidation hypothesis of disease, optimal antioxidant regimens that block oxidative reactions in animals and humans need to be identified...
  40. ncbi Cimetidine does not prevent lung injury in newborn premature infants
    Robert B Cotton
    Department of Pediatrics, Vanderbilt University, Nashville, Tennessee 37232, USA
    Pediatr Res 59:795-800. 2006
    ..We conclude that cimetidine does not reduce lung injury in newborn premature infants receiving O2 and mechanical ventilation. It is possible that cimetidine was not an adequate CYP inhibitor in this context...
  41. ncbi Urinary F2-isoprostane metabolite levels in children with sleep-disordered breathing
    Hawley E Montgomery-Downs
    Department of Psychology, West Virginia University, Morgantown, WV 26506-6040, USA
    Sleep Breath 10:211-5. 2006
    ..The absence of increased levels of urinary F2-isoprostane metabolites in children with sleep-disordered breathing suggests that oxidative stress is not a significant feature of pediatric sleep-disordered breathing...
  42. ncbi Inhaled nitric oxide attenuates hyperoxic lung injury in lambs
    Robert B Cotton
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Pediatr Res 59:142-6. 2006
    ..However, this study provides no direct evidence that NO is inhibiting CYP-mediated oxidant lung injury...
  43. ncbi Oxidative stress and multi-organ failure in hospitalized elderly people
    James S Powers
    J Am Geriatr Soc 56:1150-2. 2008
  44. ncbi The in vivo gene expression signature of oxidative stress
    Eun Soo Han
    Department of Biological Science, University of Tulsa, Tulsa, Oklahoma, USA
    Physiol Genomics 34:112-26. 2008
    ..A retrospective comparison with previous studies shows that induction of these p53 target genes is a conserved expression response to oxidative stress, in vivo and in vitro, in different species and different cells/organs...
  45. ncbi The Shanghai Women's Asthma and Allergy Study: objectives, design, and recruitment results
    Tina V Hartert
    Division of Allergy, Pulmonary and Critical Care Medicine, School of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 8300, USA
    Am J Epidemiol 167:1387-96. 2008
    ..This paper describes the study objectives, design, population demographics, and recruitment results...
  46. ncbi Inhibition of heme protein redox cycling: reduction of ferryl heme by iron chelators and the role of a novel through-protein electron transfer pathway
    L Jackson Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    Free Radic Biol Med 44:257-60. 2008
  47. ncbi Potential role of isoketals formed via the isoprostane pathway of lipid peroxidation in ischemic arrhythmias
    Penelope A Boyden
    Department of Pharmacology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    J Cardiovasc Pharmacol 50:480-6. 2007
    ..Furthermore, we review what is known about the generation of such in the highly remodeled post myocardial infarction substrate. We suggest prevention of adduction of these highly reactive compounds would be antiarrhythmic...
  48. ncbi Measurement of products of docosahexaenoic acid peroxidation, neuroprostanes, and neurofurans
    Kyle O Arneson
    Division of Clinical Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Methods Enzymol 433:127-43. 2007
    ..This review outlines in detail proper sample handling, extraction and hydrolysis of the F(4)-NPs and NFs from tissue membrane phospholipids or biological fluids, and purification and derivatization of the compounds for analysis by GC/MS...
  49. ncbi Seizure-induced formation of isofurans: novel products of lipid peroxidation whose formation is positively modulated by oxygen tension
    Manisha Patel
    Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Neurochem 104:264-70. 2008
    ..These results demonstrate seizure-induced increase in IsoF formation and its correlation with changes in hippocampal pO2 and mitochondrial dysfunction...
  50. ncbi The relationship between dose of vitamin E and suppression of oxidative stress in humans
    L Jackson Roberts
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    Free Radic Biol Med 43:1388-93. 2007
    ..This study provides information on the dosage of vitamin E that decreases systemic oxidant stress in vivo in humans and informs the planning and evaluation of clinical studies that assess the efficacy of vitamin E to mitigate disease...
  51. ncbi Reduction in glutathione peroxidase 4 increases life span through increased sensitivity to apoptosis
    Qitao Ran
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, TX, USA
    J Gerontol A Biol Sci Med Sci 62:932-42. 2007
    ..Our data indicate that lifelong reduction in Gpx4 increased life span and reduced/retarded age-related pathology most likely through alterations in sensitivity of tissues to apoptosis...
  52. ncbi Insights into oxidative stress: the isoprostanes
    Paolo Montuschi
    Department of Pharmacology, Faculty of Medicine, Catholic University of the Sacred Heart, Largo F Vito 1, 00168 Rome, Italy
    Curr Med Chem 14:703-17. 2007
    ..Measurement of F(2)-IsoPs may provide a uniquely valuable approach to understanding of the clinical pharmacology of antioxidants...
  53. ncbi Pyridoxamine analogues scavenge lipid-derived gamma-ketoaldehydes and protect against H2O2-mediated cytotoxicity
    Sean S Davies
    Departments of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232, USA
    Biochemistry 45:15756-67. 2006
    ....
  54. ncbi Stability of measurements of biomarkers of oxidative stress in blood over 36 hours
    Tianying Wu
    Department of Nutrition, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    Cancer Epidemiol Biomarkers Prev 13:1399-402. 2004
    ..45 for 0 to 24 hours and 0.09 for 0 to 36 hours. We conclude that measurements of fluorescent oxidation products in blood remain stable for up to 36 hours and may be used in large prospective epidemiologic studies of chronic diseases...
  55. ncbi A nomenclature system for isofurans
    Douglass F Taber
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    Prostaglandins Other Lipid Mediat 73:47-50. 2004
    ..As a result, there is a need for a systematic nomenclature for this class of natural products. A facile system that will allow the ready differentiation of each of the isomeric structures comprising the family of isofurans is presented...
  56. ncbi Levuglandinyl adducts of proteins are formed via a prostaglandin H2 synthase-dependent pathway after platelet activation
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    J Biol Chem 278:16926-8. 2003
    ..They also demonstrate that a physiological stimulus is sufficient to lead to the lipid modification of proteins through the levuglandin pathway in human platelets...
  57. ncbi Characterization of an animal model of postmenopausal hypertension in spontaneously hypertensive rats
    Lourdes A Fortepiani
    Department of Physiology and Biophysics, University of Mississippi Medical Center, 2500 N State St, Jackson, MS 39216-4505, USA
    Hypertension 41:640-5. 2003
    ..These data suggest that the PMR rats, but not ovx rats, may be a suitable model for the study of postmenopausal hypertension, and that oxidative stress plays a role in the increased BP...
  58. ncbi Discovery of lipid peroxidation products formed in vivo with a substituted tetrahydrofuran ring (isofurans) that are favored by increased oxygen tension
    Joshua P Fessel
    Departments of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 99:16713-8. 2002
    ....
  59. ncbi The isoprostanes: their role as an index of oxidant stress status in human pulmonary disease
    Jason D Morrow
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    Am J Respir Crit Care Med 166:S25-30. 2002
    ....
  60. ncbi Identification of oxidized derivatives of neuroketals
    Nathalie Bernoud-Hubac
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232-6602, USA
    Biochemistry 41:11466-71. 2002
    ....
  61. ncbi Embryonic fibroblasts from Gpx4+/- mice: a novel model for studying the role of membrane peroxidation in biological processes
    Qitao Ran
    Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio TX 78229 3900, USA
    Free Radic Biol Med 35:1101-9. 2003
    ..Our data demonstrate that MEFs from Gpx4(+/-) mice are more sensitive to oxidative stress because of reduced expression of PHGPx...
  62. ncbi Peripheral F2-isoprostanes and F4-neuroprostanes are not increased in Alzheimer's disease
    Thomas J Montine
    Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Ann Neurol 52:175-9. 2002
    ..These results should guide the organization of clinical trials now being planned for patients with Alzheimer's disease...
  63. ncbi Superoxide dismutase and oxidative stress in Dahl salt-sensitive and -resistant rats
    Shumei Meng
    Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA
    Am J Physiol Regul Integr Comp Physiol 283:R732-8. 2002
    ..05). In conclusion, salt-sensitive hypertension in the S rat is accompanied by marked decreases in renal medullary SOD and greater renal oxidative stress and renal damage than in R rats...
  64. ncbi Herpes simplex virus type 1 encephalitis is associated with elevated levels of F2-isoprostanes and F4-neuroprostanes
    Dejan Milatovic
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    J Neurovirol 8:295-305. 2002
    ....
  65. ncbi Effects of reactive gamma-ketoaldehydes formed by the isoprostane pathway (isoketals) and cyclooxygenase pathway (levuglandins) on proteasome function
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 27232-6602, USA
    FASEB J 16:715-7. 2002
    ..These findings suggest that IsoKs/NeuroKs/LGs can inhibit proteasome activity and, if overproduced, may have relevance to the pathogenesis of neurodegenerative diseases...
  66. ncbi Dietary flavonoids fail to suppress F2-isoprostane formation in vivo
    Joye K Willcox
    Department of Food Science, North Carolina State University, Raleigh, NC 27695-7624, USA
    Free Radic Biol Med 34:795-9. 2003
    ..We conclude that under these experimental conditions, quercetin, catechin, and epicatechin do not suppress lipid peroxidation in vivo...
  67. ncbi Enzyme immunoassays for 15-F2T isoprostane-M, an urinary biomarker for oxidant stress
    Diane M Sasaki
    Oxford Biomedical Research, P.O. Box 522, Oxford, MI 48371, USA
    Adv Exp Med Biol 507:537-41. 2002
  68. ncbi Isoketals: highly reactive gamma-ketoaldehydes formed from the H2-isoprostane pathway
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
    Chem Phys Lipids 128:85-99. 2004
    ....
  69. ncbi The biochemistry of the isoprostane, neuroprostane, and isofuran pathways of lipid peroxidation
    L Jackson Roberts
    Departments of Pharmacology and Medicine, 522 RRB, Vanderbilt University, Nashville, TN 37232 6602, USA
    Chem Phys Lipids 128:173-86. 2004
    ..Oxygen concentrations differentially modulate the formation of isoprostanes and isofurans; at elevated oxygen concentrations, the formation of isofurans is favored whereas the formation of isoprostanes is disfavored...
  70. ncbi Isoprostanes and related products of lipid peroxidation in neurodegenerative diseases
    Kathleen S Montine
    Department of Pathology, University of Washington, Harborview Medical Center, Box 359791, 325 9th Ave, Seattle, WA 98104, USA
    Chem Phys Lipids 128:117-24. 2004
    ..These results indicate that brain lipid peroxidation is a potential therapeutic target early in the course of AD and HD, that CSF IsoPs may aid in the assessment of anti-oxidant experimental therapeutics and laboratory diagnosis of AD...
  71. ncbi Characterization of bis(levuglandinyl) urea derivatives as products of the reaction between prostaglandin H2 and arginine
    Irene Zagol-Ikapitte
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232-6602, USA
    Biochemistry 43:5503-10. 2004
    ..The soluble characteristic of these molecules provides a potential strategy for development of biological markers of lipid modification of proteins following cyclooxygenase activity or lipid peroxidation...
  72. ncbi Pyridoxamine: an extremely potent scavenger of 1,4-dicarbonyls
    Venkataraman Amarnath
    Departments of Pathology and Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Chem Res Toxicol 17:410-5. 2004
    ..Only 1,4-dicarbonyls are likely to participate in the proposed mechanism, thereby conferring unique sensitivity of this class of compounds to scavenging by PM...
  73. ncbi Treatment with a catalytic antioxidant corrects the neurobehavioral defect in ataxia-telangiectasia mice
    Susan E Browne
    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, NY, USA
    Free Radic Biol Med 36:938-42. 2004
    ..We show that treatment with a catalytic antioxidant corrects the neurobehavioral deficit in these mice...
  74. ncbi Modification of proteins by isoketal-containing oxidized phospholipids
    Cynthia J Brame
    Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, Tennessee 27232-6602, USA
    J Biol Chem 279:13447-51. 2004
    ....
  75. ncbi Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin gamma-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 27232 6602, USA
    Nat Protoc 2:2079-91. 2007
    ..IsoK/LG-lysyl-lactam adducts are measured by liquid chromatography tandem mass spectrometry after proteolytic digestion of extracted proteins, solid-phase extraction and preparative HPLC...
  76. ncbi Isofurans, but not F2-isoprostanes, are increased in the substantia nigra of patients with Parkinson's disease and with dementia with Lewy body disease
    Joshua P Fessel
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Neurochem 85:645-50. 2003
    ....
  77. ncbi Localization of isoketal adducts in vivo using a single-chain antibody
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
    Free Radic Biol Med 36:1163-74. 2004
    ..We conclude that isoketal adducts form in epithelial cells as a result of high oxygen exposure and that this single-chain antibody provides a valuable tool to localize the formation of isoketal adducts in tissues in vivo...
  78. ncbi Mass spectrometric quantification of F2-isoprostanes as indicators of oxidant stress
    Jason D Morrow
    Departments of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, TN, USA
    Methods Mol Biol 186:57-66. 2002
  79. ncbi Formation of highly reactive A-ring and J-ring isoprostane-like compounds (A4/J4-neuroprostanes) in vivo from docosahexaenoic acid
    Samuel S Fam
    Departments of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 277:36076-84. 2002
    ..Thus, these studies have identified novel, highly reactive A/J-ring isoprostane-like compounds that are derived from docosahexaenoic acid in vivo...
  80. ncbi Suppression of atherogenesis by overexpression of glutathione peroxidase-4 in apolipoprotein E-deficient mice
    Zhongmao Guo
    Department of Cardiovascular Biology, Meharry Medical College, Nashville, TN 37208, USA
    Free Radic Biol Med 44:343-52. 2008
    ..These results suggest that overexpression of GPx4 inhibits the development of atherosclerosis by decreasing lipid peroxidation and inhibiting the sensitivity of vascular cells to oxidized lipids...
  81. ncbi Evidence of oxidative stress in full-term healthy infants
    James K Friel
    Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
    Pediatr Res 56:878-82. 2004
    ..5 mo. These data suggest that the human infant is under oxidative stress early in infancy and further study may be warranted to assess the potential benefits of antioxidant supplementation for either the mother or the infant...
  82. ncbi Lipid peroxidation in aging brain and Alzheimer's disease
    Thomas J Montine
    Department of Pharmacology, Pathology, and Medicine and the Vanderbilt Brain Institute, Vanderbilt University Medical Center, Nashville, TN, USA
    Free Radic Biol Med 33:620-6. 2002
    ....
  83. ncbi Superoxide formation and interaction with nitric oxide modulate systemic arterial pressure and renal function in salt-depleted dogs
    Utpal K Dutta
    Department of Physiology, SL 39, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112, USA
    Exp Biol Med (Maywood) 231:269-76. 2006
    ..These data show that low salt intake enhances O(2)(-) activity that influences renal and systemic hemodynamics and thus may contribute to the regulation of arterial pressure in the salt-restricted state...
  84. ncbi The cyclopentenone product of lipid peroxidation, 15-A(2t)-isoprostane (8-isoprostaglandin A(2)), is efficiently conjugated with glutathione by human and rat glutathione transferase A4-4
    Ina Hubatsch
    Department of Biochemistry, Uppsala University, SE-75123 Uppsala, Sweden
    Chem Res Toxicol 15:1114-8. 2002
    ..These studies have therefore defined a potentially important route by which cyclopentenone IsoPs are metabolized that may serve as a mechanism for the inactivation of these highly reactive compounds...
  85. ncbi Aging, resting metabolic rate, and oxidative damage: results from the Louisiana Healthy Aging Study
    Madlyn I Frisard
    Pennington Biomedical Research Center, 6400 Perkins Rd, Baton Rouge, LA 70808, USA
    J Gerontol A Biol Sci Med Sci 62:752-9. 2007
    ..The current study was designed to test whether nonagenarians have a relatively low metabolic rate and whether it is associated with low levels of oxidative stress relative to age...
  86. ncbi Ethanol self-administration and alterations in the livers of the cynomolgus monkey, Macaca fascicularis
    Priscilla Ivester
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Alcohol Clin Exp Res 31:144-55. 2007
    ....
  87. ncbi Hydrolysis of bimatoprost (Lumigan) to its free acid by ocular tissue in vitro
    Sean S Davies
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Ocul Pharmacol Ther 19:45-54. 2003
    ..Our findings suggest that bimatoprost can act as prodrug for FP receptor activation and questions the concept of a "prostamide receptor" agonist...
  88. ncbi Association between low serum enterolactone and increased plasma F2-isoprostanes, a measure of lipid peroxidation
    Meri Vanharanta
    Research Institute of Public Health, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland
    Atherosclerosis 160:465-9. 2002
    ..Our present data suggest that low serum enterolactone concentration is associated with enhanced in vivo lipid peroxidation in men...