David Piston

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. pmc Regulation of beta cell glucokinase by S-nitrosylation and association with nitric oxide synthase
    Mark A Rizzo
    Dept of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 735 Light Hall, Nashville, TN 37232, USA
    J Cell Biol 161:243-8. 2003
  2. pmc Photoconversion in orange and red fluorescent proteins
    Gert Jan Kremers
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
    Nat Methods 6:355-8. 2009
  3. pmc Glucose decouples intracellular Ca2+ activity from glucagon secretion in mouse pancreatic islet alpha-cells
    Sylvain J Le Marchand
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
    PLoS ONE 7:e47084. 2012
  4. pmc Minireview: Dopaminergic regulation of insulin secretion from the pancreatic islet
    Alessandro Ustione
    Department of Molecular Physiology and Biophysics, Vanderbilt University, 702 Light Hall, Nashville, Tennessee 37232 0615, USA
    Mol Endocrinol 27:1198-207. 2013
  5. pmc Neuropeptide Y and somatostatin inhibit insulin secretion through different mechanisms
    Tara A Schwetz
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Am J Physiol Endocrinol Metab 304:E211-21. 2013
  6. pmc Dopamine synthesis and D3 receptor activation in pancreatic β-cells regulates insulin secretion and intracellular [Ca(2+)] oscillations
    Alessandro Ustione
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232 0615, USA
    Mol Endocrinol 26:1928-40. 2012
  7. ncbi request reprint The coming of age of two-photon excitation imaging for intravital microscopy
    David W Piston
    Vanderbilt University, Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 702 Light Hall, Nashville, TN 37232, USA
    Adv Drug Deliv Rev 58:770-2. 2006
  8. ncbi request reprint Fluorescent protein FRET: the good, the bad and the ugly
    David W Piston
    Department of Molecular Physiology and Biophysics, Vanderbilt University, 702 Light Hall, Nashville, TN 37232 0615, USA
    Trends Biochem Sci 32:407-14. 2007
  9. pmc When two is better than one: elements of intravital microscopy
    David W Piston
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    PLoS Biol 3:e207. 2005
  10. pmc Glucose suppression of glucagon secretion: metabolic and calcium responses from alpha-cells in intact mouse pancreatic islets
    Sylvain J Le Marchand
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 285:14389-98. 2010

Research Grants

  1. Dopamine action in pancreatic islet function
    David W Piston; Fiscal Year: 2010
  2. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2009
  3. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2007
  4. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2004
  5. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2003
  6. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 1999
  7. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2001
  8. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2002
  9. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David W Piston; Fiscal Year: 2010
  10. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2000

Collaborators

Detail Information

Publications55

  1. pmc Regulation of beta cell glucokinase by S-nitrosylation and association with nitric oxide synthase
    Mark A Rizzo
    Dept of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 735 Light Hall, Nashville, TN 37232, USA
    J Cell Biol 161:243-8. 2003
    ....
  2. pmc Photoconversion in orange and red fluorescent proteins
    Gert Jan Kremers
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
    Nat Methods 6:355-8. 2009
    ..The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date...
  3. pmc Glucose decouples intracellular Ca2+ activity from glucagon secretion in mouse pancreatic islet alpha-cells
    Sylvain J Le Marchand
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
    PLoS ONE 7:e47084. 2012
    ..We conclude that glucose suppression of glucagon secretion is not mediated by inactivation of calcium channels, but instead, it requires a calcium-independent inhibitory pathway...
  4. pmc Minireview: Dopaminergic regulation of insulin secretion from the pancreatic islet
    Alessandro Ustione
    Department of Molecular Physiology and Biophysics, Vanderbilt University, 702 Light Hall, Nashville, Tennessee 37232 0615, USA
    Mol Endocrinol 27:1198-207. 2013
    ....
  5. pmc Neuropeptide Y and somatostatin inhibit insulin secretion through different mechanisms
    Tara A Schwetz
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Am J Physiol Endocrinol Metab 304:E211-21. 2013
    ..GPCR ligands signal through multiple pathways to inhibit insulin secretion, and determining these mechanisms could lead to novel diabetic therapies...
  6. pmc Dopamine synthesis and D3 receptor activation in pancreatic β-cells regulates insulin secretion and intracellular [Ca(2+)] oscillations
    Alessandro Ustione
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232 0615, USA
    Mol Endocrinol 26:1928-40. 2012
    ..Because blocking dopaminergic negative feedback increases insulin secretion, expanding the knowledge of this pathway in β-cells might offer a potential new target for the treatment of type 2 diabetes...
  7. ncbi request reprint The coming of age of two-photon excitation imaging for intravital microscopy
    David W Piston
    Vanderbilt University, Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 702 Light Hall, Nashville, TN 37232, USA
    Adv Drug Deliv Rev 58:770-2. 2006
  8. ncbi request reprint Fluorescent protein FRET: the good, the bad and the ugly
    David W Piston
    Department of Molecular Physiology and Biophysics, Vanderbilt University, 702 Light Hall, Nashville, TN 37232 0615, USA
    Trends Biochem Sci 32:407-14. 2007
    ..We also review the approaches that can be used to measure FRET, with particular emphasis on the potential artifacts associated with each approach...
  9. pmc When two is better than one: elements of intravital microscopy
    David W Piston
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    PLoS Biol 3:e207. 2005
  10. pmc Glucose suppression of glucagon secretion: metabolic and calcium responses from alpha-cells in intact mouse pancreatic islets
    Sylvain J Le Marchand
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 285:14389-98. 2010
    ..Taken together, the data suggest that suppression occurs downstream from alpha-cell calcium signaling, presumably at the level of vesicle trafficking or exocytotic machinery...
  11. pmc Glucose-dependent blood flow dynamics in murine pancreatic islets in vivo
    Lara R Nyman
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Am J Physiol Endocrinol Metab 298:E807-14. 2010
    ..These results indicate that glucose differentially regulates blood flow in the pancreatic islet vasculature independently of blood flow in the rest of the pancreas...
  12. ncbi request reprint Substrate binding stoichiometry and kinetics of the norepinephrine transporter
    Joel W Schwartz
    Center for Molecular Neuroscience, Vanderbilt University Medical Center, Nashville, Tennessee 37232 8548, USA
    J Biol Chem 280:19177-84. 2005
    ..Therefore binding has a low probability of resulting in transport. We interpret these data to mean that inefficient binding could contribute to slow transport rates...
  13. ncbi request reprint Quantitative NAD(P)H/flavoprotein autofluorescence imaging reveals metabolic mechanisms of pancreatic islet pyruvate response
    Jonathan V Rocheleau
    Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 279:31780-7. 2004
    ..Overall, these data are consistent with either a restricted pyruvate dehydrogenase-dependent metabolism or a buffering of the NADH response by other metabolic mechanisms...
  14. ncbi request reprint Pancreatic islet beta-cells transiently metabolize pyruvate
    Jonathan V Rocheleau
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232 0615, USA
    J Biol Chem 277:30914-20. 2002
    ..Based on these data, we propose a mechanism for glucose-stimulated insulin secretion that includes allosteric inhibition of tricarboxylic acid cycle enzymes and pH dependence of mitochondrial pyruvate transport...
  15. pmc Establishment and quantitative imaging of a 3D lung organotypic model of mammary tumor outgrowth
    Michelle D Martin
    Department of Cancer Biology, Vanderbilt University, 771 Preston Research Building, 2220 Pierce Avenue, Nashville, TN 37212, USA
    Clin Exp Metastasis 25:877-85. 2008
    ..In summary, we have developed a novel in vitro model of breast tumor cells in a common metastatic site that can be used to study tumor/host interactions in an important microenvironment...
  16. pmc Gap junction coupling and calcium waves in the pancreatic islet
    Richard K P Benninger
    Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
    Biophys J 95:5048-61. 2008
    ..The resulting quantitative agreement between the data and analytical models of islet connectivity, using only a single free parameter, reveals the mechanistic underpinnings of the multicellular behavior of the islet...
  17. pmc Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets
    Lara R Nyman
    Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA
    J Clin Invest 118:3790-7. 2008
    ..Our approach included both millisecond temporal resolution and submicron spatial resolution, allowing for real-time imaging of islet blood flow within the living mouse, which has not to our knowledge been attainable by other methods...
  18. ncbi request reprint Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction
    Mingming Hao
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Diabetes 56:2328-38. 2007
    ..In this study, we examined the relationship between cholesterol and insulin secretion from pancreatic beta-cells that is independent of the effects of FFAs...
  19. pmc Subcutaneous transplantation of embryonic pancreas for correction of type 1 diabetes
    Subhadra C Gunawardana
    Dept of Molecular Physiology and Biophysics, Vanderbilt Univ School of Medicine, Nashville, TN 37232, USA
    Am J Physiol Endocrinol Metab 296:E323-32. 2009
    ..Thus, this strategy is a convenient alternative to islet transplantation in diabetic mice and has the potential to be translated to human clinical applications with appropriate modifications...
  20. pmc Control of glucose phosphorylation in L6 myotubes by compartmentalization, hexokinase, and glucose transport
    Richard R Whitesell
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 6303, USA
    Biochem J 370:47-56. 2003
    ..Insulin activation of glucose phosphorylation might then result from stimulation of these transporters together with HK recruitment or relief from inhibition by G6P...
  21. pmc Critical role of gap junction coupled KATP channel activity for regulated insulin secretion
    Jonathan V Rocheleau
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    PLoS Biol 4:e26. 2006
    ..Control via coupling lessens the effects of cell-cell variation and provides resistance to defects in excitability that would otherwise lead to a profound diabetic state, such as occurs in persistent neonatal diabetes mellitus...
  22. ncbi request reprint Regulation of two insulin granule populations within the reserve pool by distinct calcium sources
    Mingming Hao
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Cell Sci 118:5873-84. 2005
    ..Our study provides a model by which glucose and other secretory stimuli can regulate the readily releasable pool through the same mechanisms that regulate insulin secretion...
  23. pmc Development of a novel fluorogenic proteolytic beacon for in vivo detection and imaging of tumour-associated matrix metalloproteinase-7 activity
    J Oliver McIntyre
    Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 6840, U S A
    Biochem J 377:617-28. 2004
    ..60%. Thus PB-M7VIS functions as a PB for in vivo detection of MMP-7 activity that serves to light this optical beacon and is, therefore, a selective in vivo optical molecular imaging contrast reagent...
  24. ncbi request reprint Oxidative stress is a mediator of glucose toxicity in insulin-secreting pancreatic islet cell lines
    Lan Wu
    Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, 715 PRB, Vanderbilt University Medical Center, 2220 Pierce Avenue, Nashville, TN 37232, USA
    J Biol Chem 279:12126-34. 2004
    ..The level of beta cell GK may also be critical in this process...
  25. pmc Rapid extravasation and establishment of breast cancer micrometastases in the liver microenvironment
    Michelle D Martin
    Department of Cancer Biology, Vanderbilt University, Nashville, TN 37212, USA
    Mol Cancer Res 8:1319-27. 2010
    ....
  26. pmc Deletion of the mouse Slc30a8 gene encoding zinc transporter-8 results in impaired insulin secretion
    Lynley D Pound
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, TN 37232, USA
    Biochem J 421:371-6. 2009
    ..In summary, Slc30a8 gene deletion is accompanied by a modest impairment in insulin secretion without major alterations in glucose metabolism...
  27. pmc Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy
    Richard K P Benninger
    Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA
    Opt Lett 33:2895-7. 2008
    ..We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration...
  28. pmc Quantitative measurement of zinc secretion from pancreatic islets with high temporal resolution using droplet-based microfluidics
    Christopher J Easley
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Vanderbilt University, 702 Light Hall, Nashville, Tennessee 37232, USA
    Anal Chem 81:9086-95. 2009
    ..This droplet sampling technique should be widely applicable to time-resolved cellular secretion measurements, either in real-time or for postprocessing...
  29. pmc Selective visualization of cyclooxygenase-2 in inflammation and cancer by targeted fluorescent imaging agents
    Md Jashim Uddin
    AB Hancock, Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Cancer Res 70:3618-27. 2010
    ....
  30. pmc Intrasequence GFP in class I MHC molecules, a rigid probe for fluorescence anisotropy measurements of the membrane environment
    Jonathan V Rocheleau
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232, USA
    Biophys J 84:4078-86. 2003
    ..These results demonstrate the feasibility and usefulness of a GFP moiety rigidly attached to the protein of interest as a probe for molecular motion and proximity in cell membranes...
  31. ncbi request reprint Imaging Beta cell development in real-time using pancreatic explants from mice with green fluorescent protein-labeled pancreatic Beta cells
    Subhadra C Gunawardana
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 735 Light Hall, Nashville, Tennessee 37232, USA
    In Vitro Cell Dev Biol Anim 41:7-11. 2005
    ..We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells...
  32. ncbi request reprint Mechanisms of time-dependent potentiation of insulin release: involvement of nitric oxide synthase
    Subhadra C Gunawardana
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA
    Diabetes 55:1029-33. 2006
    ..The addition of arginine to wild-type islets also enabled glucose to induce TDP. Our results show that nNOS activity contributes to the absence of TDP in mouse islets putatively through depletion of intracellular arginine...
  33. doi request reprint Dimethyl amiloride improves glucose homeostasis in mouse models of type 2 diabetes
    Subhadra C Gunawardana
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Am J Physiol Endocrinol Metab 294:E1097-108. 2008
    ..Thus we also have explored another possible mechanism through which DMA enables/enhances TDP, via the activation of mitochondrial alpha-ketoglutarate dehydrogenase...
  34. ncbi request reprint FRET by fluorescence polarization microscopy
    David W Piston
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Methods Cell Biol 85:415-30. 2008
    ..In this chapter, we will discuss the theory behind AFRET imaging, calculation of FP anisotropies using fluorescent microscopes, and configuration of microscopes for AFRET experiments...
  35. ncbi request reprint A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin
    Mark A Rizzo
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Biol Chem 277:34168-75. 2002
    ..Together, these data support a model for post-translational regulation of GK whereby insulin regulates both the association of GK with secretory granules and the activity of the enzyme within the pancreatic beta-cell...
  36. pmc Microfluidic glucose stimulation reveals limited coordination of intracellular Ca2+ activity oscillations in pancreatic islets
    Jonathan V Rocheleau
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232 0615, USA
    Proc Natl Acad Sci U S A 101:12899-903. 2004
    ....
  37. ncbi request reprint Reduction in pancreatic transcription factor PDX-1 impairs glucose-stimulated insulin secretion
    Marcela Brissova
    Veterans Affairs Tennessee Valley Healthcare System, Nashville, Tennessee 37212, USA
    J Biol Chem 277:11225-32. 2002
    ....
  38. ncbi request reprint Leptin requires canonical migratory signaling pathways for induction of monocyte and macrophage chemotaxis
    Marnie L Gruen
    Vanderbilt University Medical Center, Department of Molecular Physiology and Biophysics, Nashville, Tennessee, USA
    Am J Physiol Cell Physiol 293:C1481-8. 2007
    ..These data have implications for the impact of hyperleptinemia on obesity-related pathophysiological conditions such as diabetes, cardiovascular disease, and cancer...
  39. ncbi request reprint Impaired glucagon secretory responses in mice lacking the type 1 sulfonylurea receptor
    Chiyo Shiota
    Vanderbilt University School of Medicine, Dept of Molecular Physiology and Biophysics, Nashville, TN 37232 0615, USA
    Am J Physiol Endocrinol Metab 289:E570-7. 2005
    ..These findings indicate that K(ATP) channels in alpha-cells play a key role in regulating glucagon secretion, thereby adding to the paradox of how mice that lack K(ATP) channels maintain euglycemia...
  40. pmc Flecainide inhibits arrhythmogenic Ca2+ waves by open state block of ryanodine receptor Ca2+ release channels and reduction of Ca2+ spark mass
    Fredrick A Hilliard
    Division of Clinical Pharmacology, Oates Institute for Experimental Therapeutics, Vanderbilt University School of Medicine, Nashville, TN 37232 0575, USA
    J Mol Cell Cardiol 48:293-301. 2010
    ..Our results indicate that inhibition of the RyR2 open state provides a new therapeutic strategy to prevent diastolic Ca(2+) waves resulting in triggered arrhythmias, such as CPVT...
  41. doi request reprint Two-photon excitation microscopy for the study of living cells and tissues
    Jonathan V Rocheleau
    Vanderbilt University, Nashville, Tennessee, USA
    Curr Protoc Cell Biol . 2003
    ..The advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate localized photochemistry...
  42. pmc Rapid and inexpensive fabrication of polymeric microfluidic devices via toner transfer masking
    Christopher J Easley
    Department of Molecular Physiology, Vanderbilt University Medical Center, Vanderbilt University, 747D Light Hall, 21st Avenue South, Nashville, TN 37232 0615, USA
    Lab Chip 9:1119-27. 2009
    ....
  43. pmc Kinetics of a collagen-like polypeptide fragmentation after mid-IR free-electron laser ablation
    Andrey Zavalin
    Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee 37235, USA
    Biophys J 95:1371-81. 2008
    ....
  44. pmc Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells
    Cunxi Li
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Mol Biol Cell 18:3081-93. 2007
    ....
  45. pmc High-contrast imaging of fluorescent protein FRET by fluorescence polarization microscopy
    Mark A Rizzo
    Biophys J 88:L14-6. 2005
    ..This approach is able to distinguish FRET between linked and unlinked Cerulean and Venus fluorescent proteins in living cells with a larger dynamic range than other approaches...
  46. ncbi request reprint An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004
    ..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
  47. ncbi request reprint Imaging pancreatic beta-cells in the intact pancreas
    Manami Hara
    Dept of Medicine, University of Chicago, 5841 South Maryland Ave, MC1027, Chicago, IL 60637, USA
    Am J Physiol Endocrinol Metab 290:E1041-7. 2006
    ....
  48. ncbi request reprint Optimization of pairings and detection conditions for measurement of FRET between cyan and yellow fluorescent proteins
    Mark A Rizzo
    University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Microsc Microanal 12:238-54. 2006
    ..FRET was also detected by measuring the acceptor fluorescence anisotropy. Although difficult to quantify, this method is exceptional for screening purposes, because it provides high contrast for discriminating FRET...
  49. doi request reprint Turning fluorescent proteins into energy-saving light bulbs
    Gert Jan Kremers
    Nat Methods 5:472-3. 2008
  50. ncbi request reprint X-ray structure of Cerulean GFP: a tryptophan-based chromophore useful for fluorescence lifetime imaging
    Gabrielle D Malo
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287 1604, USA
    Biochemistry 46:9865-73. 2007
    ..Possible interpretations in terms of chromophore isomerization are presented...
  51. pmc Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye
    Yoshikazu Imanishi
    Department of Ophthalmology, University of Washington, 1959 NE Pacific St, Box 356485, Seattle, WA 98195 6485, USA
    J Cell Biol 164:373-83. 2004
    ..These results indicate that RESTs located close to the RPE plasma membrane are essential components in 11-cis-retinal production...
  52. pmc Chemically resolved imaging of biological cells and thin films by infrared scanning near-field optical microscopy
    Antonio Cricenti
    Istituto di Stuttura della Materia, Rome, Italy
    Biophys J 85:2705-10. 2003
    ..The potential applications of this approach touch virtually every aspect of the life sciences and medical research, as well as problems in materials science, chemistry, physics, and environmental research...
  53. pmc Optical lock-in detection of FRET using synthetic and genetically encoded optical switches
    Shu Mao
    Department of Physiology, University of Wisconsin Madison, Madison, Wisconsin, USA
    Biophys J 94:4515-24. 2008
    ....
  54. ncbi request reprint Regulation of corepressor function by nuclear NADH
    Qinghong Zhang
    Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
    Science 295:1895-7. 2002
    ..We propose that this ability to detect changes in nuclear NAD+/NADH ratio allows CtBP to serve as a redox sensor for transcription...

Research Grants19

  1. Dopamine action in pancreatic islet function
    David W Piston; Fiscal Year: 2010
    ....
  2. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2009
    ..Second, understanding the cell-to-cell communication pathways may lead to new drugs that enhance insulin secretion for the treatment of Type II diabetes. ..
  3. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2007
    ..Second, understanding the cell-to-cell communication pathways may lead to new drugs that enhance insulin secretion for the treatment of Type II diabetes. ..
  4. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2004
    ..abstract_text> ..
  5. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2003
    ..abstract_text> ..
  6. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 1999
    ..The applicant concludes that these investigations will advance our understanding of the in vivo interplay of biochemical mechanisms known to be involved in glucose-stimulated insulin secretion from pancreatic islets. ..
  7. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2001
    ..The applicant concludes that these investigations will advance our understanding of the in vivo interplay of biochemical mechanisms known to be involved in glucose-stimulated insulin secretion from pancreatic islets. ..
  8. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2002
    ..abstract_text> ..
  9. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David W Piston; Fiscal Year: 2010
    ..Second, understanding the cell-to-cell communication pathways may lead to new drugs that enhance insulin secretion for the treatment of Type II diabetes. ..
  10. DYNAMICS OF PANCREATIC ISLET FUNCTION
    David Piston; Fiscal Year: 2000
    ..The applicant concludes that these investigations will advance our understanding of the in vivo interplay of biochemical mechanisms known to be involved in glucose-stimulated insulin secretion from pancreatic islets. ..
  11. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2004
    ..abstract_text> ..
  12. VANDERBILT IN VIVO IMAGING CENTER
    David Piston; Fiscal Year: 2002
    ..Collaborations between projects and cores promise to validate current hypotheses about tumor vascularization, and to rapidly apply these finding to clinical cancer diagnosis and therapy. ..
  13. Two-photon FCS System
    David Piston; Fiscal Year: 2005
    ..This analysis will greatly facilitate all of our on-going projects. Finally, the open Alba data structure allows other analysis methods to be applied as they are developed. ..
  14. Probes for Quantitative Optical and Electron Microscopy
    David Piston; Fiscal Year: 2007
    ..This research will provide tools for describing cell physiology in terms of specific molecular dynamics and interactions, which will lead us into the next frontier of structural cell biology. ..
  15. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2002
    ..abstract_text> ..
  16. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2003
    ..abstract_text> ..
  17. Dynamic of Pancreatic Islet Function
    David Piston; Fiscal Year: 2005
    ..abstract_text> ..