Hassane McHaourab


Affiliation: Vanderbilt University
Country: USA


  1. Kusakizako T, Claxton D, Tanaka Y, Maturana A, Kuroda T, Ishitani R, et al. Structural Basis of H+-Dependent Conformational Change in a Bacterial MATE Transporter. Structure. 2018;: pubmed publisher
    ..Based on the present results, we propose a common step in the transport cycle shared among prokaryotic H+-coupled MATE transporters. ..
  2. Warren G, Stein R, McHaourab H, Eichman B. Movement of the RecG Motor Domain upon DNA Binding Is Required for Efficient Fork Reversal. Int J Mol Sci. 2018;19: pubmed publisher
    ..Together, this work helps provide a more coherent model of fork binding and remodeling by RecG and related eukaryotic enzymes. ..
  3. Hustedt E, Marinelli F, Stein R, Faraldo Gómez J, McHaourab H. Confidence Analysis of DEER Data and Its Structural Interpretation with Ensemble-Biased Metadynamics. Biophys J. 2018;115:1200-1216 pubmed publisher
    ..After careful benchmarks, we demonstrate the proposed techniques using DEER results from spin-labeled T4 lysozyme. ..
  4. McHaourab H, Lin Y, Spiller B. Crystal structure of an activated variant of small heat shock protein Hsp16.5. Biochemistry. 2012;51:5105-12 pubmed publisher
    ..5 oligomer flexibility revealed here, which is likely to be highly conserved across the sHSP superfamily, explains the relationship between oligomer expansion observed in disease-linked mutants and changes in chaperone activity. ..
  5. Mishra S, Chandler S, WILLIAMS D, Claxton D, Koteiche H, Stewart P, et al. Engineering of a Polydisperse Small Heat-Shock Protein Reveals Conserved Motifs of Oligomer Plasticity. Structure. 2018;26:1116-1126.e4 pubmed publisher
  6. Kazmier K, Sharma S, Islam S, Roux B, McHaourab H. Conformational cycle and ion-coupling mechanism of the Na+/hydantoin transporter Mhp1. Proc Natl Acad Sci U S A. 2014;111:14752-7 pubmed publisher
    ..Comparative analysis of ligand-dependent alternating access in LeuT and Mhp1 lead us to propose that different coupling schemes to ion gradients may define distinct conformational mechanisms within the LeuT-fold class. ..
  7. Claxton D, Jagessar K, Steed P, Stein R, McHaourab H. Sodium and proton coupling in the conformational cycle of a MATE antiporter from Vibrio cholerae. Proc Natl Acad Sci U S A. 2018;115:E6182-E6190 pubmed publisher
  8. Claxton D, Kazmier K, Mishra S, McHaourab H. Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes Using Spin Labeling and EPR Spectroscopy. Methods Enzymol. 2015;564:349-87 pubmed publisher
  9. Dastvan R, Fischer A, Mishra S, Meiler J, McHaourab H. Protonation-dependent conformational dynamics of the multidrug transporter EmrE. Proc Natl Acad Sci U S A. 2016;113:1220-5 pubmed publisher

More Information


  1. McHaourab H, Godar J, Stewart P. Structure and mechanism of protein stability sensors: chaperone activity of small heat shock proteins. Biochemistry. 2009;48:3828-37 pubmed publisher
  2. McHaourab H, Kumar M, Koteiche H. Specificity of alphaA-crystallin binding to destabilized mutants of betaB1-crystallin. FEBS Lett. 2007;581:1939-43 pubmed
    ..In the lens, where alpha-crystallin binding sites are not regenerated, this may represent an added mechanism to maintain lens transparency. ..
  3. Wu S, Zou P, Fuller A, Mishra S, Wang Z, Schey K, et al. Expression of Cataract-linked ?-Crystallin Variants in Zebrafish Reveals a Proteostasis Network That Senses Protein Stability. J Biol Chem. 2016;291:25387-25397 pubmed
    ..Furthermore, our work implicates additional inputs/factors in this underlying proteostasis network and demonstrates the utility of zebrafish as a platform to delineate mechanisms of cataract. ..
  4. Kazmier K, Claxton D, McHaourab H. Alternating access mechanisms of LeuT-fold transporters: trailblazing towards the promised energy landscapes. Curr Opin Struct Biol. 2017;45:100-108 pubmed publisher
    ..Although these studies have illuminated multiple aspects of transporter structures and dynamics, a number of questions remain unresolved that so far hinder understanding transport mechanisms in an energy landscape perspective. ..
  5. Collauto A, Mishra S, Litvinov A, McHaourab H, Goldfarb D. Direct Spectroscopic Detection of ATP Turnover Reveals Mechanistic Divergence of ABC Exporters. Structure. 2017;25:1264-1274.e3 pubmed publisher
    ..The integrated spectroscopic approach presented here, which link energy input to conformational dynamics, can be applied to a variety of systems powered by ATP turnover. ..
  6. Mishra S, Wu S, Fuller A, Wang Z, Rose K, Schey K, et al. Loss of αB-crystallin function in zebrafish reveals critical roles in the development of the lens and stress resistance of the heart. J Biol Chem. 2018;293:740-753 pubmed publisher
  7. Zou P, Wu S, Koteiche H, Mishra S, Levic D, KNAPIK E, et al. A conserved role of αA-crystallin in the development of the zebrafish embryonic lens. Exp Eye Res. 2015;138:104-13 pubmed publisher
  8. Mishra S, Verhalen B, Stein R, Wen P, Tajkhorshid E, McHaourab H. Conformational dynamics of the nucleotide binding domains and the power stroke of a heterodimeric ABC transporter. elife. 2014;3:e02740 pubmed publisher
    ..These findings provide direct evidence of divergence in the mechanism of ABC exporters.DOI: http://dx.doi.org/10.7554/eLife.02740.001. ..
  9. McHaourab H, Steed P, Kazmier K. Toward the fourth dimension of membrane protein structure: insight into dynamics from spin-labeling EPR spectroscopy. Structure. 2011;19:1549-61 pubmed publisher
    ..Efforts to develop methods for determining structures from EPR restraints and to increase sensitivity and throughput promise to expand spin labeling applications in membrane protein structural biology. ..
  10. McHaourab H, Mishra S, Koteiche H, Amadi S. Role of sequence bias in the topology of the multidrug transporter EmrE. Biochemistry. 2008;47:7980-2 pubmed publisher
    ..We propose a coupled insertion and assembly model for EmrE in which the favorable energetics of the parallel dimer interface override topological constraints arising from weak asymmetry in positive charge distribution. ..