Lawrence J Marnett

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi Endogenous DNA damage and mutation
    L J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt Ingram Cancer Center, Center in Molecular Toxicology, Dept of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Trends Genet 17:214-21. 2001
  2. ncbi Oxy radicals, lipid peroxidation and DNA damage
    Lawrence J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research, Center in Molecular Toxicology and The Vanderbilt Cancer Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Toxicology 181:219-22. 2002
  3. ncbi Recent developments in cyclooxygenase inhibition
    Lawrence J Marnett
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37215, USA
    Prostaglandins Other Lipid Mediat 68:153-64. 2002
  4. ncbi COX-2: a target for colon cancer prevention
    Lawrence J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research, Center in Molecular Toxicology, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Annu Rev Pharmacol Toxicol 42:55-80. 2002
  5. pmc Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein
    Lawrence J Marnett
    Department of Biochemistry, Vanderbilt University School of Medicine, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Nashville, Tennessee, USA
    J Clin Invest 111:583-93. 2003
  6. ncbi Cyclooxygenase mechanisms
    L J Marnett
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Curr Opin Chem Biol 4:545-52. 2000
  7. ncbi Regulation of prostaglandin biosynthesis by nitric oxide is revealed by targeted deletion of inducible nitric-oxide synthase
    L J Marnett
    Department of Biochemistry and Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 275:13427-30. 2000
  8. ncbi Cyclooxygenase 2 inhibitors: discovery, selectivity and the future
    L J Marnett
    Departments of Biochemistry and Chemistry, Center in Molecular Toxicology and Vanderbilt Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Trends Pharmacol Sci 20:465-9. 1999
  9. ncbi Design of selective inhibitors of cyclooxygenase-2 as nonulcerogenic anti-inflammatory agents
    L J Marnett
    AB Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Curr Opin Chem Biol 2:482-90. 1998
  10. ncbi Lipid peroxidation-DNA damage by malondialdehyde
    L J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research Center in Molecular Toxicology, Vanderbilt Cancer Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville TN 37232, USA
    Mutat Res 424:83-95. 1999

Detail Information

Publications120 found, 100 shown here

  1. ncbi Endogenous DNA damage and mutation
    L J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt Ingram Cancer Center, Center in Molecular Toxicology, Dept of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Trends Genet 17:214-21. 2001
    ..This article highlights recent discoveries and emerging opportunities in the study of endogenous DNA damage and mutation...
  2. ncbi Oxy radicals, lipid peroxidation and DNA damage
    Lawrence J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research, Center in Molecular Toxicology and The Vanderbilt Cancer Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Toxicology 181:219-22. 2002
    ..Lipid peroxidation appears to be a major source of endogenous DNA damage in humans that may contribute significantly to cancer and other genetic diseases linked to lifestyle and dietary factors...
  3. ncbi Recent developments in cyclooxygenase inhibition
    Lawrence J Marnett
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37215, USA
    Prostaglandins Other Lipid Mediat 68:153-64. 2002
    ..New strategies for the development of COX-2-selective inhibitors are highlighted...
  4. ncbi COX-2: a target for colon cancer prevention
    Lawrence J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research, Center in Molecular Toxicology, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Annu Rev Pharmacol Toxicol 42:55-80. 2002
    ..It is hoped that, as the genome sequence is understood more clearly, other targets will emerge that will provide even more effective drugs for future cancer prevention...
  5. pmc Endogenous generation of reactive oxidants and electrophiles and their reactions with DNA and protein
    Lawrence J Marnett
    Department of Biochemistry, Vanderbilt University School of Medicine, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Nashville, Tennessee, USA
    J Clin Invest 111:583-93. 2003
  6. ncbi Cyclooxygenase mechanisms
    L J Marnett
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Curr Opin Chem Biol 4:545-52. 2000
    ....
  7. ncbi Regulation of prostaglandin biosynthesis by nitric oxide is revealed by targeted deletion of inducible nitric-oxide synthase
    L J Marnett
    Department of Biochemistry and Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 275:13427-30. 2000
    ..These studies support the hypothesis that NO and/or NO-derived species modulate cyclooxygenase activity and eicosanoid production in vivo...
  8. ncbi Cyclooxygenase 2 inhibitors: discovery, selectivity and the future
    L J Marnett
    Departments of Biochemistry and Chemistry, Center in Molecular Toxicology and Vanderbilt Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Trends Pharmacol Sci 20:465-9. 1999
    ..In this review, some key points and unresolved issues related to the discovery of COX-2 inhibitors, the kinetic and structural basis for their selectivity, and possible complications in their development and use will be discussed...
  9. ncbi Design of selective inhibitors of cyclooxygenase-2 as nonulcerogenic anti-inflammatory agents
    L J Marnett
    AB Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Curr Opin Chem Biol 2:482-90. 1998
    ..Preclinical and clinical studies suggest cyclooxygenase-2 inhibitors are highly promising new agents for the treatment of pain and inflammation, and for the prevention of cancer...
  10. ncbi Lipid peroxidation-DNA damage by malondialdehyde
    L J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research Center in Molecular Toxicology, Vanderbilt Cancer Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville TN 37232, USA
    Mutat Res 424:83-95. 1999
    ..High throughput methods for its detection and quantitation will be extremely useful for screening large populations...
  11. pmc Structural and functional analysis of Sulfolobus solfataricus Y-family DNA polymerase Dpo4-catalyzed bypass of the malondialdehyde-deoxyguanosine adduct
    Robert L Eoff
    Department of Chemistry, A B Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 48:7079-88. 2009
    ..The results are consistent with the reported mutagenicity of M1dG and illustrate how the lesion may affect replication events...
  12. ncbi Amino acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid anandamide
    Kevin R Kozak
    Department of Biochemistry, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 42:9041-9. 2003
    ..Coupled with earlier observations with the endocannabinoid 2-arachidonylglycerol, these results indicate that one possible function of the highly conserved COX-2 active site side pocket is to promote endocannabinoid oxygenation...
  13. ncbi A novel mechanism of cyclooxygenase-2 inhibition involving interactions with Ser-530 and Tyr-385
    Scott W Rowlinson
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 278:45763-9. 2003
    ..Mutagenesis experiments suggest Ser-530 is also important in time-dependent inhibition by nimesulide and piroxicam...
  14. pmc Translesion DNA synthesis by human DNA polymerase eta on templates containing a pyrimidopurinone deoxyguanosine adduct, 3-(2'-deoxy-beta-d-erythro-pentofuranosyl)pyrimido-[1,2-a]purin-10(3H)-one
    Jennifer B Stafford
    Department of Chemistry, A B Hancock, Jr, Memorial Laboratory for Cancer Research, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 48:471-80. 2009
    ..Human DNA polymerase eta bypass may lead to M(1)dG to dT and frameshift but likely not M(1)dG to dA mutations during DNA replication...
  15. pmc Genetic loss of Faah compromises male fertility in mice
    Xiaofei Sun
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Biol Reprod 80:235-42. 2009
    ..Collectively, the results show that aberrant endocannabinoid signaling via CNR1 impairs normal sperm function. Besides unveiling a new regulatory mechanism of sperm function, this study has clinical significance in male fertility...
  16. pmc Prostaglandin H synthase-2-catalyzed oxygenation of 2-arachidonoylglycerol is more sensitive to peroxide tone than oxygenation of arachidonic acid
    Joel Musee
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 287:37383-94. 2012
    ..GPx4 silencing led to 2-4-fold increases in PG-G formation but no change in PG formation. Thus, cellular peroxide tone may be an important determinant of the extent of endocannabinoid oxygenation by PGHS-2...
  17. ncbi Inhibition of cyclooxygenase with indomethacin phenethylamide reduces atherosclerosis in apoE-null mice
    Michael E Burleigh
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232 6300, USA
    Biochem Pharmacol 70:334-42. 2005
    ....
  18. pmc Oxidation and glycolytic cleavage of etheno and propano DNA base adducts
    Charles G Knutson
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 48:800-9. 2009
    ..These multiple pathways of biotransformation produce an array of products. Thus, the biotransformation of exocyclic adducts may lead to an additional class of biomarkers suitable for use in animal and human studies...
  19. pmc The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase
    Zheyong Yu
    Department of Pharmacology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 100:9162-7. 2003
    ..Our results provide strong biochemical evidence for a functional linkage of 12R-LOX and eLOX3 and clues into skin biochemistry and the etiology of ichthyosiform diseases in humans...
  20. pmc Light-induced isomerization of apoptolidin a leads to inversion of C2-C3 double bond geometry
    Brian O Bachmann
    Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee 77842 3012, USA
    Org Lett 12:2944-7. 2010
    ..The isolation, characterization, and cytotoxicity against H292 cells of apoptolidin G are reported. Apoptolidin G is shown to be derived by a light-induced isomerization of the C2-C3 carbon-carbon double bond of apoptolidin A...
  21. pmc Differential sensitivity and mechanism of inhibition of COX-2 oxygenation of arachidonic acid and 2-arachidonoylglycerol by ibuprofen and mefenamic acid
    Jeffery J Prusakiewicz
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 48:7353-5. 2009
    ..In contrast, ibuprofen and mefenamate must bind in both subunits to inhibit AA binding...
  22. ncbi Differential DNA recognition and cleavage by EcoRI dependent on the dynamic equilibrium between the two forms of the malondialdehyde-deoxyguanosine adduct
    Laurie A VanderVeen
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 44:5024-33. 2005
    ..Comparison of the solution structures of DNA adducts and the crystal structure of EcoRI complexed to substrate suggest a model to explain the functional differences...
  23. pmc In vitro bypass of the major malondialdehyde- and base propenal-derived DNA adduct by human Y-family DNA polymerases κ, ι, and Rev1
    Leena Maddukuri
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 49:8415-24. 2010
    ..The results indicate that DNA hPol κ or the combined action of hPol ι or Rev1 and hPol κ bypass M(1)dG residues in DNA and generate products that are consistent with some of the mutations induced by M(1)dG in mammalian cells...
  24. ncbi Structure of the 1,N(2)-propanodeoxyguanosine adduct in a three-base DNA hairpin loop derived from a palindrome in the Salmonella typhimurium hisD3052 gene
    Jason P Weisenseel
    Department of Chemistry, Center in Molecular Toxicology, A B Hancock, Jr, Memorial Laboratory for Cancer Research, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, Tennessee 37235, USA
    Chem Res Toxicol 15:140-52. 2002
    ..The geometry of this three-base loop is similar to that of other DNA hairpins containing three-base loops, and suggests a common motif for the folding of these loops...
  25. pmc Differential regulation of endocannabinoid synthesis and degradation in the uterus during embryo implantation
    Haibin Wang
    Department of Pediatrics, Division of Reproductive and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Prostaglandins Other Lipid Mediat 83:62-74. 2007
    ..The results suggest that aberrant functioning of these pathways impacting uterine anandamide and/or 2-AG levels would compromise pregnancy outcome...
  26. ncbi Metabolism of the endocannabinoids, 2-arachidonylglycerol and anandamide, into prostaglandin, thromboxane, and prostacyclin glycerol esters and ethanolamides
    Kevin R Kozak
    Department of Biochemistry, Vanderbilt Ingram Cancer Center, and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 277:44877-85. 2002
    ..These results define the in vitro diversity of endocannabinoid-derived prostanoids and will permit focused investigations into their production and potential biological actions in vivo...
  27. pmc Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurine
    MELISSA V TURMAN
    A B Hancock, Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 47:3917-25. 2008
    ..Over prolonged incubations, RPMs also generated small amounts of diHETE-T. Oxidative metabolism of polyunsaturated N-acyltaurines may represent a pathway for the generation or termination of novel signaling molecules...
  28. pmc (R)-Profens are substrate-selective inhibitors of endocannabinoid oxygenation by COX-2
    Kelsey C Duggan
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Nat Chem Biol 7:803-9. 2011
    ....
  29. ncbi Kinetic and thermodynamic analysis of the hydrolytic ring-opening of the malondialdehyde-deoxyguanosine adduct, 3-(2'-deoxy-beta-D-erythro-pentofuranosyl)- pyrimido[1,2-alpha]purin-10(3H)-one
    James N Riggins
    A B Hancock Jr Memorial Laboratory for Cancer Research, Departments of Biochemistry and Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Am Chem Soc 126:8237-43. 2004
    ..A mechanism is proposed for ring-opening of M1dG under basic conditions and a role is proposed for duplex DNA in accelerating the rate of ring-opening of M1dG at neutral pH...
  30. pmc Fatty acid amide hydrolase deficiency limits early pregnancy events
    Haibin Wang
    Department of Pediatrics, Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    J Clin Invest 116:2122-31. 2006
    ..This study uncovers what we believe to be a novel regulation of preimplantation processes, which could be clinically relevant for fertility regulation in women...
  31. ncbi Kinetics and mechanism of the general-acid-catalyzed ring-closure of the malondialdehyde-DNA adduct, N2-(3-oxo-1-propenyl)deoxyguanosine (N2OPdG-), to 3-(2'-Deoxy-beta-D-erythro-pentofuranosyl)pyrimido[1,2-alpha]purin- 10(3H)-one (M1dG)
    James N Riggins
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Am Chem Soc 126:10571-81. 2004
    ..This work details the complexity of ring-closure in the nucleoside and oligonucleotides and provides new insight into the role of duplex DNA in catalyzing ring-opening and ring-closing of M1dG and N2OPdG...
  32. ncbi In vitro bypass of malondialdehyde-deoxyguanosine adducts: differential base selection during extension by the Klenow fragment of DNA polymerase I is the critical determinant of replication outcome
    Muhammed F Hashim
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 43:11828-35. 2004
    ..They also provide a detailed picture of in vitro replication in which the outcome is determined primarily by the selectivity of template-primer extension beyond rather than insertion opposite the adducts...
  33. pmc Molecular basis for cyclooxygenase inhibition by the non-steroidal anti-inflammatory drug naproxen
    Kelsey C Duggan
    AB Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute for Chemical Biology, the Center in Molecular Toxicology, Nashville, Tennessee 37232 0146
    J Biol Chem 285:34950-9. 2010
    ..7 and 2.3 Å resolution, respectively. The combination of mutagenesis, structure analysis, and x-ray crystallography provided comprehensive information on the unique interactions responsible for naproxen binding to COX-2...
  34. pmc In vivo oxidative metabolism of a major peroxidation-derived DNA adduct, M1dG
    Michael B Otteneder
    Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Proc Natl Acad Sci U S A 103:6665-9. 2006
    ..6-Oxo-M1dG may be a useful biomarker of endogenous DNA damage associated with inflammation, oxidative stress, and certain types of cancer chemotherapy...
  35. pmc Zymosan-induced glycerylprostaglandin and prostaglandin synthesis in resident peritoneal macrophages: roles of cyclo-oxygenase-1 and -2
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Biochem J 399:91-9. 2006
    ..They also indicate that the 2-AG and AA used for PG-G and PG synthesis respectively are derived from independent pathways...
  36. ncbi Metabolism in vitro and in vivo of the DNA base adduct, M1G
    Charles G Knutson
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Chem Res Toxicol 20:550-7. 2007
    ..Alternative repair pathways or biological processing of M1dG makes the fate of M1G of interest as a potential marker of oxidative damage in vivo...
  37. pmc Selective visualization of cyclooxygenase-2 in inflammation and cancer by targeted fluorescent imaging agents
    Md Jashim Uddin
    AB Hancock, Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Cancer Res 70:3618-27. 2010
    ....
  38. pmc Bulge migration of the malondialdehyde OPdG DNA adduct when placed opposite a two-base deletion in the (CpG)3 frameshift hotspot of the Salmonella typhimurium hisD3052 gene
    Yazhen Wang
    Departments of Chemistry and Biochemistry, Institute of Chemical Biology, Center in Molecular Toxicology, A B Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN 37235, USA
    Chem Res Toxicol 20:1200-10. 2007
    ..Both samples attained equilibrium in approximately 140 days at pH 7 and 25 degrees C...
  39. pmc Substrate-selective COX-2 inhibition decreases anxiety via endocannabinoid activation
    Daniel J Hermanson
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Nat Neurosci 16:1291-8. 2013
    ..Our data suggest a key role for COX-2 in the regulation of eCB signaling and indicate that substrate-selective pharmacology represents a viable approach for eCB augmentation with broad therapeutic potential. ..
  40. ncbi Glycerylprostaglandin synthesis by resident peritoneal macrophages in response to a zymosan stimulus
    Carol A Rouzer
    Department of Biochemistry, the Vanderbilt Institute of Chemical Biology, the Center in Molecular Toxicology, Nashville, TN 37232 0146, USA
    J Biol Chem 280:26690-700. 2005
    ..In conclusion, lipopolysaccharide-pretreated macrophages produce PG-Gs from endogenous 2-AG during zymosan phagocytosis, but PG-G formation is limited by substrate hydrolysis and inactivation of COX-2...
  41. pmc The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737
    Mariana Boiani
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 288:6980-90. 2013
    ..These studies identify BAG3-mediated Mcl-1 stabilization as a potential target for cancer drug discovery...
  42. pmc Cyclooxygenase-1-selective inhibitors based on the (E)-2'-des-methyl-sulindac sulfide scaffold
    Andy J Liedtke
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Med Chem 55:2287-300. 2012
    ..E-DMSS analogues may be useful probes of COX-1 biology in vivo and promising leads for COX-1-targeted therapeutic agents...
  43. ncbi Selective oxygenation of N-arachidonylglycine by cyclooxygenase-2
    Jeffery J Prusakiewicz
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 296:612-7. 2002
    ..These results suggest a possible role for COX-2 in the regulation of NAGly levels and the formation of a novel class of eicosanoids from NAGly metabolism...
  44. pmc The influence of double bond geometry in the inhibition of cyclooxygenases by sulindac derivatives
    Matthew J Walters
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Bioorg Med Chem Lett 19:3271-4. 2009
    ..Thus, although the 2'-methyl group is a major determinant of time-dependent cyclooxygenase inhibition, the geometry of the benzylidene double bond plays a role as well...
  45. ncbi Lipid profiling reveals glycerophospholipid remodeling in zymosan-stimulated macrophages
    Carol A Rouzer
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 46:6026-42. 2007
    ..These results suggest that GPCho is the major ultimate source of 20:4 that is mobilized in zymosan-stimulated RPMs but that 20:4 mobilization may involve the intermediate turnover of alkyl acyl GPEtn species...
  46. ncbi N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation
    Yong Guo
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    J Biol Chem 280:23429-32. 2005
    ..The expression is well correlated with its activity and anandamide levels. This study is clinically relevant, since elevated anandamide levels in peripheral circulation are associated with spontaneous pregnancy failure in women...
  47. pmc Fluorinated COX-2 inhibitors as agents in PET imaging of inflammation and cancer
    Md Jashim Uddin
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry, Chemistry and Pharmacology, Vanderbilt Institute of Chemical Biology, Center for Molecular Toxicology, TN, USA
    Cancer Prev Res (Phila) 4:1536-45. 2011
    ..The in vitro and in vivo properties of compound 7 suggest it will be a useful probe for early detection of cancer and for evaluation of the COX-2 status of premalignant and malignant tumors...
  48. doi Progress toward the total synthesis of lucentamycin A: total synthesis and biological evaluation of 8-epi-lucentamycin A
    R Nathan Daniels
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Org Chem 74:8852-5. 2009
    ..2% overall yield. The key epi-nonproteogenic 3-methyl-4-ethylideneproline was synthesized via a titanium-mediated cycloisomerization reaction...
  49. pmc Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H(2) synthases
    Olivier Boutaud
    Department of Medicine, Vanderbilt University, Nashville, TN 37232 6602, USA
    Proc Natl Acad Sci U S A 99:7130-5. 2002
    ..Together these findings support the hypothesis that the clinical action of acetaminophen is mediated by inhibition of PGHS activity, and that hydroperoxide concentration contributes to its cellular selectivity...
  50. ncbi Exocyclic DNA lesions stimulate DNA cleavage mediated by human topoisomerase II alpha in vitro and in cultured cells
    Renier Velez-Cruz
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 44:3972-81. 2005
    ..This finding suggests that type II topoisomerases interact with exocyclic DNA lesions in physiological systems...
  51. ncbi RAW264.7 cells lack prostaglandin-dependent autoregulation of tumor necrosis factor-alpha secretion
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Lipid Res 46:1027-37. 2005
    ....
  52. ncbi Functional analysis of the molecular determinants of cyclooxygenase-2 acetylation by 2-acetoxyphenylhept-2-ynyl sulfide
    G Phillip Hochgesang
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Nashville, TN 37232 0146, USA
    Arch Biochem Biophys 409:127-33. 2003
    ..Arg-120 is proposed to fix the conformation of the active site to one that favors acetylation...
  53. ncbi Molecular basis of the time-dependent inhibition of cyclooxygenases by indomethacin
    Jeffery J Prusakiewicz
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute for Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 43:15439-45. 2004
    ..These results highlight binding of the 2'-methyl of INDO in the hydrophobic pocket as an important determinant of its time-dependent inhibition of COX enzymes...
  54. pmc Semisynthesis of 6-chloropurine-2'-deoxyriboside 5'-dimethoxytrityl 3'-(2-cyanoethyl-N,N-diisopropylamino)phosphoramidite and its use in the synthesis of fluorescently labeled oligonucleotides
    Md Jashim Uddin
    A B Hancock Jr Memorial Laboratory for Cancer Research, Departments of Biochemistry, Chemistry, and Pharmacology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Nucleosides Nucleotides Nucleic Acids 29:831-40. 2010
    ....
  55. ncbi Molecular determinants for the selective inhibition of cyclooxygenase-2 by lumiracoxib
    Anna L Blobaum
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute for Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 282:16379-90. 2007
    ..Taken together with a recent crystal structure of a lumiracoxib-COX-2 complex, the kinetic analyses presented herein of the inhibition of mutant COX-2s by lumiracoxib allows the definition of the molecular basis of COX-2 inhibition...
  56. ncbi Studies on the metabolism of the novel, selective cyclooxygenase-2 inhibitor indomethacin phenethylamide in rat, mouse, and human liver microsomes: identification of active metabolites
    Rory P Remmel
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Drug Metab Dispos 32:113-22. 2004
    ..The glucuronides of 2'hydroxy-LM-4108 and O-desmethyl-2'-hydroxy-LM-4108 were also identified in rat bile...
  57. ncbi Induction of apoptosis in colorectal carcinoma cells treated with 4-hydroxy-2-nonenal and structurally related aldehydic products of lipid peroxidation
    James D West
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University, Nashville, Tennessee 37232 0146, USA
    Chem Res Toxicol 17:453-62. 2004
    ..The results presented herein suggest that these molecules commonly activate certain signaling pathways that control cell death irrespective of their reactive properties...
  58. ncbi Cyclooxygenase-1-dependent prostaglandin synthesis modulates tumor necrosis factor-alpha secretion in lipopolysaccharide-challenged murine resident peritoneal macrophages
    Carol A Rouzer
    Departments of Biochemistry and Chemistry, Vanderbilt Institute for Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146
    J Biol Chem 279:34256-68. 2004
    ..These results demonstrate autocrine regulation of TNF-alpha secretion by endogenous PGs synthesized primarily by COX-1 in RPM and suggest that COX-1 may play a significant role in the regulation of the early response to endotoxemia...
  59. ncbi Identification of the protein targets of the reactive metabolite of teucrin A in vivo in the rat
    Alexandra Druckova
    Department of Biochemistry, A B Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Chem Res Toxicol 20:1393-408. 2007
    ....
  60. pmc HSF1-mediated BAG3 expression attenuates apoptosis in 4-hydroxynonenal-treated colon cancer cells via stabilization of anti-apoptotic Bcl-2 proteins
    Aaron T Jacobs
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 284:9176-83. 2009
    ..Overall, our data reveal that BAG3 is HSF1-inducible and has a unique role facilitating cancer cell survival during pro-apoptotic stress by stabilizing the level of Bcl-2 family proteins...
  61. pmc Site-specific synthesis of oligonucleotides containing malondialdehyde adducts of deoxyguanosine and deoxyadenosine via a postsynthetic modification strategy
    Hao Wang
    Department of Chemistry and Biochemistry, Center in Molecular Toxicology and Vanderbilt Institute of Chemical Biology, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    Chem Res Toxicol 19:1467-74. 2006
    ..The stability of the modified oligonucleotides was examined by UV thermal melting studies (Tm). In contrast to the M1dG adduct, OPdA caused very little change in the Tm...
  62. pmc Insertion of dNTPs opposite the 1,N2-propanodeoxyguanosine adduct by Sulfolobus solfataricus P2 DNA polymerase IV
    Yazhen Wang
    Department of Chemistry, Center in Molecular Toxicology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Biochemistry 47:7322-34. 2008
    ..These results provide insight into how -1 frameshift mutations might be generated for the PdG adduct, a structural model for the exocylic M 1dG adduct formed by malondialdehyde...
  63. ncbi Metabolism and elimination of the endogenous DNA adduct, 3-(2-deoxy-beta-D-erythropentofuranosyl)-pyrimido[1,2-alpha]purine-10(3H)-one, in the rat
    Charles G Knutson
    A B Hancock, Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 282:36257-64. 2007
    ..Additionally, both M1dG and 6-oxo-M1dG exhibited a long residence time following administration (>48 h), and the major species observed in urine at late collections was 6-oxo-M1dG...
  64. pmc Chemical properties of oxopropenyl adducts of purine and pyrimidine nucleosides and their reactivity toward amino acid cross-link formation
    Joseph Szekely
    Department of Chemistry, Center in Molecular Toxicology, Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee 37235 1822, USA
    J Am Chem Soc 130:2195-201. 2008
    ..The results define the acidity of oxopropenyl deoxynucleosides and highlight its importance to their reactivity toward nucleophiles. This study also identifies the structures of a potential novel class of DNA-protein cross-links...
  65. ncbi Indolyl esters and amides related to indomethacin are selective COX-2 inhibitors
    Amit S Kalgutkar
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry and Chemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Bioorg Med Chem 13:6810-22. 2005
    ..7. Overall, this strategy broadens the scope of our previous methodology of neutralizing the carboxylic acid group in NSAIDs as a means of generating COX-2-selective inhibitors and is potentially applicable to other NSAIDs...
  66. pmc Combined chemical and biosynthetic route to access a new apoptolidin congener
    Victor P Ghidu
    Department of Chemistry, Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Org Lett 11:3032-4. 2009
    ....
  67. ncbi Modulation of DNA fragmentation factor 40 nuclease activity by poly(ADP-ribose) polymerase-1
    James D West
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology and the Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 280:15141-7. 2005
    ..Our results suggest that PARP-1 poly(ADP-ribosyl)ation is a terminal event in the apoptotic response that occurs in response to DNA fragmentation and directly influences DFF40 activity...
  68. ncbi Heat shock factor 1 attenuates 4-Hydroxynonenal-mediated apoptosis: critical role for heat shock protein 70 induction and stabilization of Bcl-XL
    Aaron T Jacobs
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 282:33412-20. 2007
    ..Overall, activation of HSF1 and stabilization of Bcl-X(L) mediate a protective response that may contribute significantly to the cellular biology of lipid peroxidation...
  69. pmc Characterization of an AM404 analogue, N-(3-hydroxyphenyl)arachidonoylamide, as a substrate and inactivator of prostaglandin endoperoxide synthase
    MELISSA V TURMAN
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 48:12233-41. 2009
    ..These studies provide additional insight into the structural requirements for substrate metabolism and inactivation of PGHS and report the first metabolism-dependent, selective inactivator of PGHS-2...
  70. ncbi Cyclooxygenase-2 promotes early atherosclerotic lesion formation in LDL receptor-deficient mice
    Michael E Burleigh
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tenn, USA
    Circulation 105:1816-23. 2002
    ....
  71. ncbi Amide derivatives of meclofenamic acid as selective cyclooxygenase-2 inhibitors
    Amit S Kalgutkar
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Bioorg Med Chem Lett 12:521-4. 2002
    ..This paper describes SAR studies involved in the transformation of the NSAID meclofenamic acid into potent and selective cyclooxygenase-2 (COX-2) inhibitors via neutralization of the carboxylate moiety in this nonselective COX inhibitor...
  72. pmc Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications
    Carol A Rouzer
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 45:14795-808. 2006
    ....
  73. ncbi Substitution of tyrosine for the proximal histidine ligand to the heme of prostaglandin endoperoxide synthase 2: implications for the mechanism of cyclooxygenase activation and catalysis
    D C Goodwin
    Departments of Biochemistry and Chemistry, Center in Molecular Toxicology, and Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 39:5422-32. 2000
    ..In addition, our findings are strongly supportive of a branched-chain mechanism of cyclooxygenase catalysis in which one activation event leads to many cyclooxygenase turnovers...
  74. ncbi Amino acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid 2-arachidonylglycerol
    K R Kozak
    Departments of Biochemistry and Chemistry, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 276:30072-7. 2001
    ..Finally, the differences in substrate binding do not alter the stereospecificity of the cyclooxygenase reaction; 2-AG-derived and arachidonic acid-derived products share identical stereochemistry...
  75. ncbi Chemical stability of 2-arachidonylglycerol under biological conditions
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Chem Phys Lipids 119:69-82. 2002
    ....
  76. doi The COXIB experience: a look in the rearview mirror
    Lawrence J Marnett
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Annu Rev Pharmacol Toxicol 49:265-90. 2009
    ..This review provides an overview of the discovery, development, and difficulties of the COXIBs, a perspective on what has been learned, and speculation on the way forward...
  77. ncbi Induction and function of lipocalin prostaglandin D synthase in host immunity
    Myungsoo Joo
    Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Immunol 179:2565-75. 2007
    ..Our study suggests a potential therapeutic usage of L-PGDS or PGD(2) against Pseudomonas pneumonia...
  78. pmc Relating protein adduction to gene expression changes: a systems approach
    Bing Zhang
    Department of Biomedical Informatics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Biosyst 7:2118-27. 2011
    ..Although developed for analyzing protein adduction data, the framework can be easily adapted for phosphoproteomics and other types of protein modification data...
  79. pmc Repeated homotypic stress elevates 2-arachidonoylglycerol levels and enhances short-term endocannabinoid signaling at inhibitory synapses in basolateral amygdala
    Sachin Patel
    Department of Psychiatry, Vanderbilt University, Nashville, TN 37212, USA
    Neuropsychopharmacology 34:2699-709. 2009
    ..We suggest stress-induced enhancement of eCB-mediated suppression of inhibitory transmission in the BLA could contribute to affective dysregulation associated with chronic stress...
  80. ncbi Oxidative metabolism of endocannabinoids by COX-2
    Kevin R Kozak
    Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Curr Pharm Des 10:659-67. 2004
    ..The available biochemical evidence supporting a role for COX-2 in endocannabinoid metabolism will be presented. Finally, the potential biological consequences of COX-2-mediated endocannabinoid oxygenation will be discussed...
  81. ncbi Thromboxane A2 is a mediator of cyclooxygenase-2-dependent endothelial migration and angiogenesis
    T O Daniel
    Department of Medicine, The Vanderbilt Center for Vascular Biology, Vanderbilt University, Nashville, Tennessee 37232, USA
    Cancer Res 59:4574-7. 1999
    ..A TXA2 agonist, U46619, reconstitutes both migration and angiogenesis responses under COX-2-inhibited conditions. These findings identify TXA2 as a COX-2 product that functions as a critical intermediary of angiogenesis...
  82. ncbi Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid
    O Boutaud
    Department of Pharmacology, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 40:6948-55. 2001
    ..The reactivity of the PGH-synthase adducts themselves is demonstrated by the formation of intermolecular cross-links...
  83. ncbi Kinetics of the interaction of nonsteroidal antiinflammatory drugs with prostaglandin endoperoxide synthase-1 studied by limited proteolysis
    A S Kalgutkar
    A B Hancock, Jr, Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 35:9076-82. 1996
    ..The results suggest that induction of trypsin resistance is a reflection of the initial association of reversible as well as irreversible inhibitors with the apoprotein...
  84. ncbi Nitric oxide trapping of tyrosyl radicals generated during prostaglandin endoperoxide synthase turnover. Detection of the radical derivative of tyrosine 385
    D C Goodwin
    Department of Biochemistry, A B Hancock, Jr Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 273:8903-9. 1998
    ..Peptide sequencing indicated that the modified residue was tyrosine 385, the source of the putative catalytically active tyrosyl radical...
  85. pmc Formation of DNA-protein cross-links between gamma-hydroxypropanodeoxyguanosine and EcoRI
    Laurie A VanderVeen
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Chem Res Toxicol 21:1733-8. 2008
    ..This work indicates that the gamma-HOPdG-EcoRI cross-link is in equilibrium with free oligonucleotide and enzyme. Reversal of cross-link formation allows EcoRI to effect enzymatic cleavage of competitor oligonucleotides...
  86. pmc Prostaglandin E2 inhibits tumor necrosis factor-alpha RNA through PKA type I
    Jennifer B Stafford
    Department of Biochemistry, Vanderbilt University School of Medicine, 23rd Avenue South at Pierce, Nashville, TN 37232 0146, USA
    Biochem Biophys Res Commun 366:104-9. 2008
    ..The mechanisms by which prostaglandins limit TNF-alpha mRNA levels may underlie endogenous regulatory mechanisms that limit inflammation, and may have important implications for understanding chronic inflammatory disease pathogenesis...
  87. pmc Non-redundant functions of cyclooxygenases: oxygenation of endocannabinoids
    Carol A Rouzer
    A B Hancock Jr Memorial Laboratory for Cancer Research, The Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Biol Chem 283:8065-9. 2008
    ..These compounds are produced in intact cells stimulated with physiological agonists and have been isolated from in vivo sources. Important concepts relevant to the hypothesis of a COX-2-selective signaling pathway are presented...
  88. ncbi Structural and functional differences between cyclooxygenases: fatty acid oxygenases with a critical role in cell signaling
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Biochem Biophys Res Commun 338:34-44. 2005
    ..These findings suggest that PG-Gs comprise a new class of lipid mediators, and that oxygenation of neutral derivatives of AA is a distinct function for the COX-2 isoform...
  89. ncbi 15-Lipoxygenase metabolism of 2-arachidonylglycerol. Generation of a peroxisome proliferator-activated receptor alpha agonist
    Kevin R Kozak
    Department of Biochemistry, Vanderbilt Ingram Cancer Center and Center in Molecular Toxicology, Nashville, Tennessee, USA
    J Biol Chem 277:23278-86. 2002
    ..The results demonstrate that 15-LOXs are capable of acting on 2-AG to provide 15-HETE-G and elucidate a potential role for endocannabinoid oxygenation in the generation of peroxisome proliferator-activated receptor alpha agonists...
  90. doi Analysis of endocannabinoids, their congeners and COX-2 metabolites
    Philip J Kingsley
    A B Hancock, Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, United States
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2746-54. 2009
    ..This review will summarize quantitative analytical methodology as reported in the literature from 1992 to present for the analysis of endocannabinoids and related compounds...
  91. ncbi Control of prostaglandin stereochemistry at the 15-carbon by cyclooxygenases-1 and -2. A critical role for serine 530 and valine 349
    Claus Schneider
    Department of Pharmacology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 277:478-85. 2002
    ..The findings may also explain the absolute conservation of Ser-530, the target of aspirin, throughout the families of cyclooxygenase enzymes...
  92. pmc Synthesis and evaluation of the cytotoxicity of apoptolidinones A and D
    Victor P Ghidu
    Department of Chemistry, Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee 37235 1822, USA
    J Org Chem 73:4949-55. 2008
    ..In contrast to apoptolidin A, the aglycones apoptolidinone A and D were shown to be noncytotoxic when evaluated against human lung cancer cells (H292)...
  93. ncbi Kinetics of inhibition of leukocyte 12-lipoxygenase by the isoform-specific inhibitor 4-(2-oxapentadeca-4-yne)phenylpropanoic acid
    John S Moody
    Department of Biochemistry, Vanderbilt Ingram Comprehensive Cancer Center and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 41:10297-303. 2002
    ....
  94. ncbi Effects of DNA structure on oxopropenylation by the endogenous mutagens malondialdehyde and base propenal
    John P Plastaras
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Center in Molecular Toxicology and the Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 41:5033-42. 2002
    ..These data suggest that steric access to the target nucleophile located in the minor groove of DNA is critical for adduct formation by the endogenous mutagens MDA and base propenals...
  95. ncbi Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells
    Laura J Niedernhofer
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 278:31426-33. 2003
    ..These experiments provide biological and biochemical evidence for the existence of MDA-induced DNA interstrand cross-links that could result from endogenous oxidative stress and likely have potent biological effects...
  96. ncbi Development of a method for determination of the malondialdehyde-deoxyguanosine adduct in urine using liquid chromatography-tandem mass spectrometry
    Michael Otteneder
    Department of Biochemistry, Center in Molecular Toxicology, and Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University, Nashville, TN 37232, USA
    Anal Biochem 315:147-51. 2003
    ..This method is easily adaptable to the analysis of M(1)GdR in DNA samples or biological fluids...
  97. ncbi Mechanism of free radical oxygenation of polyunsaturated fatty acids by cyclooxygenases
    Carol A Rouzer
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Chem Rev 103:2239-304. 2003
  98. ncbi Evaluation of the mutagenic potential of the principal DNA adduct of acrolein
    L A VanderVeen
    Department of Biochemistry, A. B. Hancock Jr. Memorial Laboratory for Cancer Research, Center in Molecular Toxicology, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 276:9066-70. 2001
    ..The combination of these studies showed that HOPdG is not miscoding in vivo at the level of sensitivity of these site-specific mutagenesis assays...
  99. ncbi Xenobiotic-metabolizing cytochromes P450 convert prostaglandin endoperoxide to hydroxyheptadecatrienoic acid and the mutagen, malondialdehyde
    J P Plastaras
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Center in Molecular Toxicology and the Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 275:11784-90. 2000
    ..These results suggest that co-expression of cyclooxygenase-2 and P450s in developing cancers may contribute to genomic instability due to production of the endogenous mutagen, MDA...
  100. ncbi The relative contribution of adduct blockage and DNA repair on template utilization during replication of 1,N2-propanodeoxyguanosine and pyrimido
    S P Fink
    A.B. Hancock, Jr, Memorial Laboratory for Cancer Research, The Vanderbilt-Ingram Cancer Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA
    Mutat Res 485:209-18. 2001
    ..The increased strand bias indicates the importance of nucleotide excision repair in the removal of PdG and M1G...
  101. ncbi Abasic sites stimulate double-stranded DNA cleavage mediated by topoisomerase II. DNA lesions as endogenous topoisomerase II poisons
    P S Kingma
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 270:21441-4. 1995
    ..These findings suggest that abasic sites represent endogenous topoisomerase II poisons and imply that anticancer drugs mimic the cleavage-enhancing actions of naturally occurring DNA lesions...